Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0010810

Physical Location & Seq

Genomic Coordinates Forward (+)

19650203 .. 19653651

Gene Structure 3449 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1121800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1398 bp

ATGAAGCTGAATTGGATGCACTGGTTCAATCACAAGCACAAGCATTTGGTGAAGTTTGGAGTTGCAGTTCTTCTGATGGGTCTTGCTTTTCGTCTCTTTCTCTCCACCTCCAATAGGTTCTCGCCCAAATTAGAAGCCCCTTTTCCTGAGAAAACTGCGGATTCAGAGCATCCAATTGCTGTGCAGTTTCCACAAAACGAAGATCAGGATGAGATACCTTTTCCCGGAAAAGAAACACAATTTCCCGAGAAAGAAACCCATTTTCCCGAGAAAACTGGGGATTCAGAACCGCCAATTTCTGGGCTAGTTCCAGAAGATGGTGATCAGGACCAGATAGCTCCATCAAATGGAAAGTGCGATATTTTCATTGGGGATTGGATTCCAAGTACTTCGGGTGCAGCTTACAATGAGAGCTGCCCCACGATTGAAAACCCTCAGAATTGCATAAGGAATGGACGGCCTGATACAGGGTATCTGTACTGGAAGTGGAAGCCACGATACTGTGATATACCAAAATTTGATCCAGGGAGATTCCTTGAAATGATGAGGAATAAATCATGGGCGTTGATTGGTGATTCAATATCTCGCAACCATGTCCAGTCGTTGCTGTGCATGCTCTCGACAGTTGAACAAGCCGTCCAAGTGTACCATGACAAGGATTACAGGTCCAGAAGATGGCACTTCCCCTCTTACAATTTTAGTGTCTCAGTTGTTTGGTCCCCATTCCTTGTCGAAGCTGCTATTTTTGAAGACTTTGATGGTGTGGCATCATCTGAAGTTGAGCTGCATCTTGACAAGCTTGATAAGAAATGGGTGGATGAGTACCACAGTTGGGACTACATGGTAATTTCAACAGGGAAATGGTTTCTCAAAACTGCAATCTACTACGAGGGCAATGAAGTTCTGGGATGCCATTACTGTCCTAAAAGGAATCTGACAGAGTTGGGGTTTGATTTTTCTTATCGCAAAGCTTTGAGCTTTGTAATGAACTTTATAGTAAAGTCAGATTACAAGGGCACAATCTTTTTTAGAACATCCACGCCTGATCACTTTGAGGGCGGGGAGTGGAATACTGGGGGAAATTGCAACAGAACGGCCCCAGCTAAAGAAGGTGAGGTTGAAGTGAAGGAAGTAAATAAGATTCTGCGTAACATTGAACTAGAGGAGTTTCAGAAGGCAGCTGCAGAAGCTTCTAAACATGGGGTGAATCTCAAACTCCTTGACCTTTTCCAACTTTCTTTGTTGAGGCCCGATGGACATCCAGGTCCATATAGACAGTATCATCCATTTGAACAAGGCAAAAACGCGAAGCTTCAGACTGATTGTTTGCATTGGTGCTTACCAGGACCTATAGAATCTTGGAACGATATATTAATGGAGATGATAGTAAATGGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0008150 GO:0008374 GO:0009987 GO:0012505 GO:0016407 GO:0016413 GO:0016740 GO:0016746 GO:0016747 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071554

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PC-Esterase PMR5N

Protein Analysis

466

Amino Acids

53.58

Weight (kDa)

5.67

Isoelectric Point (pI)

44.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMR5N	PF14416	118 - 169	2.2e-18	PMR5 N terminal Domain
PC-Esterase	PF13839	170 - 461	4.2e-92	GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010810)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G11090 AT4G23790
fragaria_vesca	FvH4_5g04110 FvH4_5g04110
malus_domestica	MD06G1102900.v1.1 MD14G1121800.v1.1
prunus_persica	Prupe.5G115700_v2.0.a1 Prupe.5G115700_v2.0.a1 Prupe.5G115700_v2.0.a1
pyrus_communis	pycom06g09750
rosa_chinensis	RchiOBHm_Chr7g0195391
rosa_laevigata	RLG00000004124
rosa_multiflora	Rmu_sc0007366.1_g000011
rosa_roxburghii	Rroxscaffold_3G00260520
rosa_rugosa	Rorug07G0023000.1
rosa_samantha	Rh7AG150300 Rh7BG151800 Rh7CG155500 Rh7DG151300
rosa_wichuraiana	Rw7G012820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1263
AccB7I	CCANNNNNTGG	4 cut(s)	299, 317, 347, 675
AccII	CGCG	1 cut(s)	1307
AciI	CCGC	3 cut(s)	158, 290, 1059
AclWI	GGATC	1 cut(s)	515
AcsI	RAATTY	1 cut(s)	515
AcuI	CTGAAG	2 cut(s)	795, 1298
AfaI	GTAC	4 cut(s)	388, 479, 647, 824
AfiI	CCNNNNNNNGG	9 cut(s)	114, 224, 299, 317, 347, 467, 655, 675, 832
AjnI	CCWGG	3 cut(s)	523, 1261, 1342
AloI	GAACNNNNNNTCC	4 cut(s)	51, 83, 621, 653
Alw26I	GTCTC	2 cut(s)	98, 709
AlwI	GGATC	1 cut(s)	515
Ama87I	CYCGRG	2 cut(s)	245, 266
AoxI	GGCC	3 cut(s)	458, 1095, 1247
ApeKI	GCWGC	6 cut(s)	398, 414, 737, 784, 1178, 1181
ApoI	RAATTY	1 cut(s)	515
ArsI	GACNNNNNNTTYG	2 cut(s)	1309, 1341
AseI	ATTAAT	1 cut(s)	1373
AspS9I	GGNCC	7 cut(s)	328, 666, 717, 1096, 1248, 1265, 1346
AsuC2I	CCSGG	1 cut(s)	225
AsuHPI	GGTGA	5 cut(s)	61, 332, 584, 1124, 1216
AvaI	CYCGRG	2 cut(s)	245, 266
AvaII	GGWCC	5 cut(s)	328, 666, 717, 1265, 1346
BaeGI	GKGCMC	1 cut(s)	1019
BarI	GAAGNNNNNNTAC	2 cut(s)	482, 514
BbsI	GAAGAC	1 cut(s)	756
BbvI	GCAGC	6 cut(s)	401, 410, 724, 771, 1168, 1190
BccI	CCATC	6 cut(s)	70, 311, 349, 669, 752, 1247
BceAI	ACGGC	3 cut(s)	473, 620, 1110
BciT130I	CCWGG	3 cut(s)	525, 1263, 1344
BclI	TGATCA	2 cut(s)	322, 1045
BcnI	CCSGG	1 cut(s)	225
BcoDI	GTCTC	2 cut(s)	98, 709
BfaI	CTAG	2 cut(s)	305, 1160
BfmI	CTRYAG	2 cut(s)	1182, 1350
BisI	GCNGC	6 cut(s)	399, 415, 738, 785, 1179, 1182
BlsI	GCNGC	6 cut(s)	400, 416, 739, 786, 1180, 1183
BmcAI	AGTACT	1 cut(s)	388
Bme1390I	CCNGG	4 cut(s)	225, 525, 1263, 1344
Bme18I	GGWCC	5 cut(s)	328, 666, 717, 1265, 1346
BmeT110I	CYCGRG	2 cut(s)	245, 266
BmgT120I	GGNCC	7 cut(s)	328, 666, 717, 1096, 1248, 1265, 1346
BmiI	GGNNCC	2 cut(s)	719, 1098
BmrFI	CCNGG	4 cut(s)	225, 525, 1263, 1344
BmrI	ACTGGG	2 cut(s)	285, 1083
BmsI	GCATC	5 cut(s)	6, 178, 776, 796, 899
BmuI	ACTGGG	2 cut(s)	285, 1083
BpiI	GAAGAC	1 cut(s)	756
BpuMI	CCSGG	1 cut(s)	225
BsaBI	GATNNNNATC	2 cut(s)	321, 1257
BsaJI	CCNNGG	1 cut(s)	524
BsaXI	ACNNNNNCTCC	2 cut(s)	51, 81
Bsc4I	CCNNNNNNNGG	9 cut(s)	114, 224, 299, 317, 347, 467, 655, 675, 832
Bse1I	ACTGG	5 cut(s)	26, 280, 485, 598, 1078
Bse3DI	GCAATG	1 cut(s)	901
Bse8I	GATNNNNATC	2 cut(s)	321, 1257
BseBI	CCWGG	3 cut(s)	525, 1263, 1344
BseDI	CCNNGG	1 cut(s)	524
BseGI	GGATG	8 cut(s)	21, 169, 214, 823, 914, 1034, 1258, 1282
BseJI	GATNNNNATC	2 cut(s)	321, 1257
BseLI	CCNNNNNNNGG	9 cut(s)	114, 224, 299, 317, 347, 467, 655, 675, 832
BseMI	GCAATG	1 cut(s)	901
BseMII	CTCAG	3 cut(s)	138, 449, 720
BseNI	ACTGG	5 cut(s)	26, 280, 485, 598, 1078
BseRI	GAGGAG	1 cut(s)	1178
BseSI	GKGCMC	1 cut(s)	1019
BseXI	GCAGC	6 cut(s)	401, 410, 724, 771, 1168, 1190
BseYI	CCCAGC	1 cut(s)	1099
BsgI	GTGCAG	2 cut(s)	203, 417
Bsh1236I	CGCG	1 cut(s)	1307
BshFI	GGCC	3 cut(s)	460, 1097, 1249
BsiHKCI	CYCGRG	2 cut(s)	245, 266
BsiSI	CCGG	1 cut(s)	225
BslFI	GGGAC	2 cut(s)	703, 848
BslI	CCNNNNNNNGG	9 cut(s)	114, 224, 299, 317, 347, 467, 655, 675, 832
BsmAI	GTCTC	2 cut(s)	98, 709
BsmBI	CGTCTC	1 cut(s)	98
BsmFI	GGGAC	2 cut(s)	703, 848
BsnI	GGCC	3 cut(s)	460, 1097, 1249
BsoBI	CYCGRG	2 cut(s)	245, 266
Bsp1286I	GDGCHC	1 cut(s)	1019
Bsp143I	GATC	4 cut(s)	202, 322, 520, 1045
BspACI	CCGC	3 cut(s)	158, 290, 1059
BspANI	GGCC	3 cut(s)	460, 1097, 1249
BspCNI	CTCAG	3 cut(s)	139, 448, 719
BspFNI	CGCG	1 cut(s)	1307
BspLI	GGNNCC	2 cut(s)	719, 1098
BspMAI	CTGCAG	1 cut(s)	1186
BspPI	GGATC	1 cut(s)	515
BsrDI	GCAATG	1 cut(s)	901
BsrI	ACTGG	5 cut(s)	26, 280, 485, 598, 1078
BssECI	CCNNGG	1 cut(s)	524
BssMI	GATC	4 cut(s)	202, 322, 520, 1045
Bst2UI	CCWGG	3 cut(s)	525, 1263, 1344
Bst4CI	ACNGT	5 cut(s)	503, 625, 830, 920, 1278
BstC8I	GCNNGC	1 cut(s)	614
BstDEI	CTNAG	3 cut(s)	147, 435, 706
BstENI	CCTNNNNNAGG	2 cut(s)	112, 465
BstF5I	GGATG	8 cut(s)	21, 169, 214, 823, 914, 1034, 1258, 1282
BstFNI	CGCG	1 cut(s)	1307
BstKTI	GATC	4 cut(s)	205, 325, 523, 1048
BstMAI	GTCTC	2 cut(s)	98, 709
BstMBI	GATC	4 cut(s)	202, 322, 520, 1045
BstMWI	GCNNNNNNNGC	2 cut(s)	613, 1187
BstNI	CCWGG	3 cut(s)	525, 1263, 1344
BstNSI	RCATGY	1 cut(s)	616
BstSCI	CCNGG	4 cut(s)	223, 523, 1261, 1342
BstSFI	CTRYAG	2 cut(s)	1182, 1350
BstSLI	GKGCMC	1 cut(s)	1019
BstUI	CGCG	1 cut(s)	1307
BstV1I	GCAGC	6 cut(s)	401, 410, 724, 771, 1168, 1190
BstV2I	GAAGAC	1 cut(s)	756
BsuRI	GGCC	3 cut(s)	460, 1097, 1249
BtsCI	GGATG	8 cut(s)	21, 169, 214, 823, 914, 1034, 1258, 1282
BtsIMutI	CAGTG	1 cut(s)	19
Cac8I	GCNNGC	1 cut(s)	614
Cfr13I	GGNCC	7 cut(s)	328, 666, 717, 1096, 1248, 1265, 1346
Csp6I	GTAC	4 cut(s)	387, 478, 646, 823
CviAII	CATG	6 cut(s)	558, 593, 613, 650, 841, 1199
CviQI	GTAC	4 cut(s)	387, 478, 646, 823
DdeI	CTNAG	3 cut(s)	147, 435, 706
DpnI	GATC	4 cut(s)	204, 324, 522, 1047
DpnII	GATC	4 cut(s)	202, 322, 520, 1045
DrdI	GACNNNNNNGTC	1 cut(s)	1263
DseDI	GACNNNNNNGTC	1 cut(s)	1263
Eco47I	GGWCC	5 cut(s)	328, 666, 717, 1265, 1346
Eco57I	CTGAAG	2 cut(s)	795, 1298
Eco88I	CYCGRG	2 cut(s)	245, 266
EcoNI	CCTNNNNNAGG	2 cut(s)	112, 465
EcoO109I	RGGNCCY	1 cut(s)	1346
EcoRII	CCWGG	3 cut(s)	523, 1261, 1342
Esp3I	CGTCTC	1 cut(s)	98
FaeI	CATG	6 cut(s)	561, 596, 616, 653, 844, 1202
FaqI	GGGAC	2 cut(s)	703, 848
FatI	CATG	6 cut(s)	557, 592, 612, 649, 840, 1198
FauI	CCCGC	1 cut(s)	1052
FbaI	TGATCA	2 cut(s)	322, 1045
Fnu4HI	GCNGC	6 cut(s)	399, 415, 738, 785, 1179, 1182
FokI	GGATG	8 cut(s)	28, 156, 221, 830, 921, 1021, 1245, 1269
Fsp4HI	GCNGC	6 cut(s)	399, 415, 738, 785, 1179, 1182
FspBI	CTAG	2 cut(s)	305, 1160
GluI	GCNGC	6 cut(s)	399, 415, 738, 785, 1179, 1182
GsaI	CCCAGC	1 cut(s)	1103
HaeIII	GGCC	3 cut(s)	460, 1097, 1249
HapII	CCGG	1 cut(s)	225
Hin1II	CATG	6 cut(s)	561, 596, 616, 653, 844, 1202
HindIII	AAGCTT	4 cut(s)	797, 969, 1188, 1310
HinfI	GANTC	9 cut(s)	161, 281, 379, 531, 575, 931, 1141, 1207, 1355
HpaII	CCGG	1 cut(s)	225
HphI	GGTGA	5 cut(s)	61, 332, 584, 1124, 1216
Hpy166II	GTNNAC	1 cut(s)	646
Hpy188I	TCNGA	9 cut(s)	75, 166, 286, 438, 775, 936, 1006, 1173, 1317
Hpy188III	TCNNGA	9 cut(s)	146, 206, 245, 266, 311, 326, 619, 669, 791
Hpy8I	GTNNAC	1 cut(s)	646
HpyAV	CCTTC	3 cut(s)	1103, 1120, 1168
HpyCH4III	ACNGT	5 cut(s)	503, 625, 830, 920, 1278
HpyF10VI	GCNNNNNNNGC	2 cut(s)	613, 1187
HpyF3I	CTNAG	3 cut(s)	147, 435, 706
Hsp92II	CATG	6 cut(s)	561, 596, 616, 653, 844, 1202
Ksp22I	TGATCA	2 cut(s)	322, 1045
Kzo9I	GATC	4 cut(s)	202, 322, 520, 1045
LmnI	GCTCC	1 cut(s)	343
Lsp1109I	GCAGC	6 cut(s)	401, 410, 724, 771, 1168, 1190
LweI	GCATC	5 cut(s)	6, 178, 776, 796, 899
MaeI	CTAG	2 cut(s)	305, 1160
MaeIII	GTNAC	1 cut(s)	1148
MalI	GATC	4 cut(s)	204, 324, 522, 1047
MboI	GATC	4 cut(s)	202, 322, 520, 1045
MboII	GAAGA	5 cut(s)	62, 212, 326, 684, 761
MfeI	CAATTG	1 cut(s)	174
MhlI	GDGCHC	1 cut(s)	1019
MmeI	TCCRAC	1 cut(s)	1255
MnlI	CCTC	9 cut(s)	118, 444, 540, 697, 883, 1048, 1108, 1156, 1239
MseI	TTAA	1 cut(s)	1373
MspA1I	CMGCKG	1 cut(s)	1181
MspI	CCGG	1 cut(s)	225
MspR9I	CCNGG	4 cut(s)	225, 525, 1263, 1344
MunI	CAATTG	1 cut(s)	174
MvaI	CCWGG	3 cut(s)	525, 1263, 1344
MvnI	CGCG	1 cut(s)	1307
MwoI	GCNNNNNNNGC	2 cut(s)	613, 1187
NciI	CCSGG	1 cut(s)	225
NdeII	GATC	4 cut(s)	202, 322, 520, 1045
NlaIII	CATG	6 cut(s)	561, 596, 616, 653, 844, 1202
NlaIV	GGNNCC	2 cut(s)	719, 1098
NspI	RCATGY	1 cut(s)	616
PaeI	GCATGC	1 cut(s)	616
PfeI	GAWTC	9 cut(s)	161, 281, 379, 531, 575, 931, 1141, 1207, 1355
PflMI	CCANNNNNTGG	4 cut(s)	299, 317, 347, 675
PfoI	TCCNGGA	1 cut(s)	223
PkrI	GCNGC	6 cut(s)	400, 416, 739, 786, 1180, 1183
PpuMI	RGGWCCY	1 cut(s)	1346
PshBI	ATTAAT	1 cut(s)	1373
Psp5II	RGGWCCY	1 cut(s)	1346
Psp6I	CCWGG	3 cut(s)	523, 1261, 1342
PspFI	CCCAGC	1 cut(s)	1099
PspGI	CCWGG	3 cut(s)	523, 1261, 1342
PspN4I	GGNNCC	2 cut(s)	719, 1098
PspPI	GGNCC	7 cut(s)	328, 666, 717, 1096, 1248, 1265, 1346
PspPPI	RGGWCCY	1 cut(s)	1346
PstI	CTGCAG	1 cut(s)	1186
PvuII	CAGCTG	1 cut(s)	1181
RsaI	GTAC	4 cut(s)	388, 479, 647, 824
RsaNI	GTAC	4 cut(s)	387, 478, 646, 823
SaqAI	TTAA	1 cut(s)	1373
SatI	GCNGC	6 cut(s)	399, 415, 738, 785, 1179, 1182
Sau3AI	GATC	4 cut(s)	202, 322, 520, 1045
Sau96I	GGNCC	7 cut(s)	328, 666, 717, 1096, 1248, 1265, 1346
ScaI	AGTACT	1 cut(s)	388
ScrFI	CCNGG	4 cut(s)	225, 525, 1263, 1344
SduI	GDGCHC	1 cut(s)	1019
SfaNI	GCATC	5 cut(s)	6, 178, 776, 796, 899
SfcI	CTRYAG	2 cut(s)	1182, 1350
SinI	GGWCC	5 cut(s)	328, 666, 717, 1265, 1346
SphI	GCATGC	1 cut(s)	616
SsiI	CCGC	3 cut(s)	158, 290, 1059
SspMI	CTAG	2 cut(s)	305, 1160
StyD4I	CCNGG	4 cut(s)	223, 523, 1261, 1342
TaaI	ACNGT	5 cut(s)	503, 625, 830, 920, 1278
TaqI	TCGA	2 cut(s)	620, 732
TatI	WGTACW	2 cut(s)	386, 477
TfiI	GAWTC	9 cut(s)	161, 281, 379, 531, 575, 931, 1141, 1207, 1355
Tru1I	TTAA	1 cut(s)	1373
Tru9I	TTAA	1 cut(s)	1373
TscAI	CASTG	1 cut(s)	26
TseI	GCWGC	6 cut(s)	398, 414, 737, 784, 1178, 1181
TspDTI	ATGAA	4 cut(s)	17, 355, 912, 1001
TspRI	CASTG	1 cut(s)	26
Van91I	CCANNNNNTGG	4 cut(s)	299, 317, 347, 675
VpaK11BI	GGWCC	5 cut(s)	328, 666, 717, 1265, 1346
VspI	ATTAAT	1 cut(s)	1373
XagI	CCTNNNNNAGG	2 cut(s)	112, 465
XapI	RAATTY	1 cut(s)	515
XceI	RCATGY	1 cut(s)	616
XspI	CTAG	2 cut(s)	305, 1160
ZrmI	AGTACT	1 cut(s)	388

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.