Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0017377

Physical Location & Seq

Genomic Coordinates Forward (+)

20329859 .. 20332019

Gene Structure 2161 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1127600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 378 bp

ATGAGTATAAACGATCTACTTCAGAAATATATGCTGAAATATTCTCTCTTGCAATGTATCTGTATATGGCAGGTTTCGAAACACATGAACCATTTGTTAAAGGCACCTTTCTGTGTACACCCAAAAACAGGTCGTGTTTGTATTCCAATTGATCCAAATCATTGTGAGGACTTTGATCCTGCTGCAGTACCAACCTTTCCACTCATTGGCGGTTTGAGAGCAGAAGGTGATAATGAATGGGACAGAACGTCACTCGGGAAATCAATCAGGTTTTTCAGATCGTCGTTTTTGCTGCCTCTGCTAAAATCTTGCAAGGAGGAGATGGAAAGTTCCTATAACGCTAAACTGCAGCAGTCGAAGAACTCCTTTAATTGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000228 GO:0000428 GO:0003674 GO:0003676 GO:0003677 GO:0003697 GO:0003824 GO:0003896 GO:0003899 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005657 GO:0005658 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006269 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019438 GO:0030880 GO:0030894 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0032993 GO:0034062 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043596 GO:0043601 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0046483 GO:0055029 GO:0061695 GO:0070013 GO:0071704 GO:0090304 GO:0097159 GO:0097747 GO:0140098 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03030 map00230 map00240 map01100 map03030

Pfam Domains

Protein Families

DNA_primase_S

Protein Analysis

126

Amino Acids

14.41

Weight (kDa)

8.56

Isoelectric Point (pI)

48.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DNA_primase_S	PF01896	24 - 51	6.9e-07	DNA primase small subunit

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0017377)

Species	Orthologous Gene IDs
malus_domestica	MD06G1072700.v1.1 MD14G1127600.v1.1
pyrus_communis	pycom16g19580
rosa_chinensis	RchiOBHm_Chr6g0245391
rosa_laevigata	RLG00000008502 RLG00000036015
rosa_multiflora	Rmu_sc0009380.1_g000010 Rmu_sc0030772.1_g000001
rosa_rugosa	Rorug05G0394000
rosa_samantha	Rh6BG026300 Rh6CG025000 Rh7DG148900
rosa_wichuraiana	Rw2G021700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	61
AccB1I	GGYRCC	1 cut(s)	103
AccB7I	CCANNNNNTGG	1 cut(s)	206
AciI	CCGC	1 cut(s)	210
AclWI	GGATC	2 cut(s)	146, 170
AcuI	CTGAAG	1 cut(s)	5
AfaI	GTAC	2 cut(s)	117, 189
AfiI	CCNNNNNNNGG	3 cut(s)	128, 206, 372
AhdI	GACNNNNNGTC	1 cut(s)	247
AlwI	GGATC	2 cut(s)	146, 170
Ama87I	CYCGRG	1 cut(s)	254
ApeKI	GCWGC	3 cut(s)	182, 292, 349
AsuHPI	GGTGA	1 cut(s)	239
AsuII	TTCGAA	1 cut(s)	77
AvaI	CYCGRG	1 cut(s)	254
BanI	GGYRCC	1 cut(s)	103
BbvI	GCAGC	3 cut(s)	169, 279, 361
BccI	CCATC	1 cut(s)	316
BfmI	CTRYAG	2 cut(s)	183, 347
BfuAI	ACCTGC	1 cut(s)	61
BisI	GCNGC	3 cut(s)	183, 293, 350
BlsI	GCNGC	3 cut(s)	184, 294, 351
BmeRI	GACNNNNNGTC	1 cut(s)	247
BmeT110I	CYCGRG	1 cut(s)	254
BmiI	GGNNCC	1 cut(s)	105
Bpu14I	TTCGAA	1 cut(s)	77
BsaBI	GATNNNNATC	1 cut(s)	156
Bsc4I	CCNNNNNNNGG	3 cut(s)	128, 206, 372
Bse3DI	GCAATG	1 cut(s)	59
Bse8I	GATNNNNATC	1 cut(s)	156
BseJI	GATNNNNATC	1 cut(s)	156
BseLI	CCNNNNNNNGG	3 cut(s)	128, 206, 372
BseMI	GCAATG	1 cut(s)	59
BseRI	GAGGAG	1 cut(s)	332
BseXI	GCAGC	3 cut(s)	169, 279, 361
BshNI	GGYRCC	1 cut(s)	103
BsiHKCI	CYCGRG	1 cut(s)	254
BslFI	GGGAC	1 cut(s)	254
BslI	CCNNNNNNNGG	3 cut(s)	128, 206, 372
BsmFI	GGGAC	1 cut(s)	254
BsoBI	CYCGRG	1 cut(s)	254
Bsp119I	TTCGAA	1 cut(s)	77
Bsp1407I	TGTACA	1 cut(s)	115
Bsp143I	GATC	4 cut(s)	13, 151, 175, 278
BspACI	CCGC	1 cut(s)	210
BspLI	GGNNCC	1 cut(s)	105
BspMAI	CTGCAG	2 cut(s)	187, 351
BspMI	ACCTGC	1 cut(s)	61
BspPI	GGATC	2 cut(s)	146, 170
BspT104I	TTCGAA	1 cut(s)	77
BspT107I	GGYRCC	1 cut(s)	103
BsrDI	GCAATG	1 cut(s)	59
BsrGI	TGTACA	1 cut(s)	115
BssMI	GATC	4 cut(s)	13, 151, 175, 278
BstAUI	TGTACA	1 cut(s)	115
BstBI	TTCGAA	1 cut(s)	77
BstKTI	GATC	4 cut(s)	16, 154, 178, 281
BstMBI	GATC	4 cut(s)	13, 151, 175, 278
BstMWI	GCNNNNNNNGC	1 cut(s)	298
BstSFI	CTRYAG	2 cut(s)	183, 347
BstV1I	GCAGC	3 cut(s)	169, 279, 361
BveI	ACCTGC	1 cut(s)	61
Csp6I	GTAC	2 cut(s)	116, 188
CviAII	CATG	1 cut(s)	85
CviQI	GTAC	2 cut(s)	116, 188
DpnI	GATC	4 cut(s)	15, 153, 177, 280
DpnII	GATC	4 cut(s)	13, 151, 175, 278
DriI	GACNNNNNGTC	1 cut(s)	247
Eam1105I	GACNNNNNGTC	1 cut(s)	247
Eco57I	CTGAAG	1 cut(s)	5
Eco88I	CYCGRG	1 cut(s)	254
FaeI	CATG	1 cut(s)	88
FaiI	YATR	7 cut(s)	8, 30, 32, 65, 67, 86, 336
FalI	AAGNNNNNCTT	1 cut(s)	350
FaqI	GGGAC	1 cut(s)	254
FatI	CATG	1 cut(s)	84
Fnu4HI	GCNGC	3 cut(s)	183, 293, 350
Fsp4HI	GCNGC	3 cut(s)	183, 293, 350
GluI	GCNGC	3 cut(s)	183, 293, 350
Hin1II	CATG	1 cut(s)	88
HphI	GGTGA	1 cut(s)	239
Hpy166II	GTNNAC	2 cut(s)	116, 118
Hpy188I	TCNGA	2 cut(s)	24, 278
Hpy188III	TCNNGA	1 cut(s)	256
Hpy8I	GTNNAC	2 cut(s)	116, 118
Hpy99I	CGWCG	1 cut(s)	286
HpyAV	CCTTC	1 cut(s)	218
HpyCH4IV	ACGT	1 cut(s)	248
HpyCH4V	TGCA	4 cut(s)	52, 185, 312, 349
HpyF10VI	GCNNNNNNNGC	1 cut(s)	298
HpySE526I	ACGT	1 cut(s)	248
Hsp92II	CATG	1 cut(s)	88
Kzo9I	GATC	4 cut(s)	13, 151, 175, 278
LpnPI	CCDG	4 cut(s)	56, 114, 192, 253
Lsp1109I	GCAGC	3 cut(s)	169, 279, 361
MaeII	ACGT	1 cut(s)	248
MaeIII	GTNAC	1 cut(s)	249
MalI	GATC	4 cut(s)	15, 153, 177, 280
MboI	GATC	4 cut(s)	13, 151, 175, 278
MboII	GAAGA	1 cut(s)	370
MfeI	CAATTG	1 cut(s)	147
MluCI	AATT	2 cut(s)	147, 370
MnlI	CCTC	3 cut(s)	160, 306, 310
MseI	TTAA	2 cut(s)	98, 369
MunI	CAATTG	1 cut(s)	147
MwoI	GCNNNNNNNGC	1 cut(s)	298
NdeII	GATC	4 cut(s)	13, 151, 175, 278
NlaIII	CATG	1 cut(s)	88
NlaIV	GGNNCC	1 cut(s)	105
NmuCI	GTSAC	1 cut(s)	249
NspV	TTCGAA	1 cut(s)	77
PflMI	CCANNNNNTGG	1 cut(s)	206
PkrI	GCNGC	3 cut(s)	184, 294, 351
PspN4I	GGNNCC	1 cut(s)	105
PstI	CTGCAG	2 cut(s)	187, 351
RsaI	GTAC	2 cut(s)	117, 189
RsaNI	GTAC	2 cut(s)	116, 188
SaqAI	TTAA	2 cut(s)	98, 369
SatI	GCNGC	3 cut(s)	183, 293, 350
Sau3AI	GATC	4 cut(s)	13, 151, 175, 278
SetI	ASST	7 cut(s)	75, 109, 133, 197, 229, 251, 272
SfcI	CTRYAG	2 cut(s)	183, 347
SfuI	TTCGAA	1 cut(s)	77
Sse9I	AATT	2 cut(s)	147, 370
SsiI	CCGC	1 cut(s)	210
SspI	AATATT	1 cut(s)	41
TaiI	ACGT	1 cut(s)	251
TaqI	TCGA	2 cut(s)	77, 356
TasI	AATT	2 cut(s)	147, 370
TatI	WGTACW	1 cut(s)	115
Tru1I	TTAA	2 cut(s)	98, 369
Tru9I	TTAA	2 cut(s)	98, 369
TseFI	GTSAC	1 cut(s)	249
TseI	GCWGC	3 cut(s)	182, 292, 349
Tsp45I	GTSAC	1 cut(s)	249
TspDTI	ATGAA	2 cut(s)	101, 249
Van91I	CCANNNNNTGG	1 cut(s)	206

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.