MD14G1138600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0016554
Physical Location & Seq
Reverse (-)
22716359 .. 22717186
828 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1138600.v1.1.491

Sequence Viewer

Length: 207 bp
ATGAAGGGCTCGGGCCCTCTGGTCGCATCGGTAGTCGCCGCCTCCACCATGGCTCTGACGACGTCGTCTTCGTCCAACCAGGGTTTTTCTTCCCCGGCGACGAAGAACGGCGGGAAAAATTCGCATACGGCGTCGGACAAGGATAAGTTCGAGCCGAGATTCGACGGTTTGAGGTTCATTGAGACGCTGGTGACGGCGCACAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

69

Amino Acids

7.09

Weight (kDa)

9.82

Isoelectric Point (pI)

35.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016554)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G42110
fragaria_vesca FvH4_5g10570 FvH4_5g10570 FvH4_5g10570
malus_domestica MD06G1122200.v1.1 MD14G1138600.v1.1
prunus_persica Prupe.5G132900_v2.0.a1 Prupe.5G132900_v2.0.a1
pyrus_communis pycom14g11390
rosa_chinensis RchiOBHm_Chr7g0187581
rosa_roxburghii Rroxscaffold_3G00267290
rosa_rugosa Rorug06G0482800
rosa_samantha Rh7AG087700 Rh7DG089900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 64
AatII GACGTC 1 cut(s) 65
AciI CCGC 2 cut(s) 39, 111
AcsI RAATTY 1 cut(s) 118
AcyI GRCGYC 2 cut(s) 62, 131
AjnI CCWGG 1 cut(s) 78
Alw26I GTCTC 1 cut(s) 176
Ama87I CYCGRG 1 cut(s) 10
AoxI GGCC 1 cut(s) 13
ApaI GGGCCC 1 cut(s) 17
ApoI RAATTY 1 cut(s) 118
ArsI GACNNNNNNTTYG 2 cut(s) 52, 84
AspLEI GCGC 1 cut(s) 199
AspS9I GGNCC 2 cut(s) 13, 14
AsuC2I CCSGG 1 cut(s) 95
AsuHPI GGTGA 1 cut(s) 202
AvaI CYCGRG 1 cut(s) 10
BaeGI GKGCMC 1 cut(s) 17
BanII GRGCYC 2 cut(s) 11, 17
BbsI GAAGAC 1 cut(s) 60
BceAI ACGGC 2 cut(s) 124, 144
BciT130I CCWGG 1 cut(s) 80
BcnI CCSGG 1 cut(s) 95
BcoDI GTCTC 1 cut(s) 176
BisI GCNGC 1 cut(s) 39
BlsI GCNGC 1 cut(s) 40
Bme1390I CCNGG 2 cut(s) 80, 95
BmeT110I CYCGRG 1 cut(s) 10
BmgT120I GGNCC 2 cut(s) 13, 14
BmiI GGNNCC 1 cut(s) 15
BmrFI CCNGG 2 cut(s) 80, 95
BmsI GCATC 1 cut(s) 35
BpiI GAAGAC 1 cut(s) 60
BpuMI CCSGG 1 cut(s) 95
BsaHI GRCGYC 2 cut(s) 62, 131
BsaJI CCNNGG 3 cut(s) 48, 79, 93
BseBI CCWGG 1 cut(s) 80
BseDI CCNNGG 3 cut(s) 48, 79, 93
BseSI GKGCMC 1 cut(s) 17
BshFI GGCC 1 cut(s) 15
BsiHKCI CYCGRG 1 cut(s) 10
BsiSI CCGG 1 cut(s) 95
BsmAI GTCTC 1 cut(s) 176
BsmBI CGTCTC 1 cut(s) 176
BsnI GGCC 1 cut(s) 15
BsoBI CYCGRG 1 cut(s) 10
Bsp120I GGGCCC 1 cut(s) 13
Bsp1286I GDGCHC 2 cut(s) 11, 17
Bsp19I CCATGG 1 cut(s) 48
BspACI CCGC 2 cut(s) 39, 111
BspANI GGCC 1 cut(s) 15
BspLI GGNNCC 1 cut(s) 15
BssECI CCNNGG 3 cut(s) 48, 79, 93
BssNI GRCGYC 2 cut(s) 62, 131
BssT1I CCWWGG 1 cut(s) 48
Bst2UI CCWGG 1 cut(s) 80
Bst4CI ACNGT 1 cut(s) 167
BstACI GRCGYC 2 cut(s) 62, 131
BstDSI CCRYGG 1 cut(s) 48
BstHHI GCGC 1 cut(s) 199
BstMAI GTCTC 1 cut(s) 176
BstNI CCWGG 1 cut(s) 80
BstSCI CCNGG 2 cut(s) 78, 93
BstSLI GKGCMC 1 cut(s) 17
BstV2I GAAGAC 1 cut(s) 60
BsuRI GGCC 1 cut(s) 15
BtgI CCRYGG 1 cut(s) 48
CfoI GCGC 1 cut(s) 199
Cfr13I GGNCC 2 cut(s) 13, 14
CseI GACGC 2 cut(s) 120, 193
CviAII CATG 1 cut(s) 49
CviJI RGCY 4 cut(s) 9, 15, 53, 154
CviKI_1 RGCY 4 cut(s) 9, 15, 53, 154
DrdI GACNNNNNNGTC 1 cut(s) 64
DseDI GACNNNNNNGTC 1 cut(s) 64
Eco130I CCWWGG 1 cut(s) 48
Eco24I GRGCYC 2 cut(s) 11, 17
Eco88I CYCGRG 1 cut(s) 10
EcoO109I RGGNCCY 1 cut(s) 14
EcoRII CCWGG 1 cut(s) 78
EcoT14I CCWWGG 1 cut(s) 48
EcoT38I GRGCYC 2 cut(s) 11, 17
ErhI CCWWGG 1 cut(s) 48
Esp3I CGTCTC 1 cut(s) 176
FaeI CATG 1 cut(s) 52
FaiI YATR 2 cut(s) 50, 126
FatI CATG 1 cut(s) 48
FauI CCCGC 1 cut(s) 104
Fnu4HI GCNGC 1 cut(s) 39
FriOI GRGCYC 2 cut(s) 11, 17
Fsp4HI GCNGC 1 cut(s) 39
GlaI GCGC 1 cut(s) 198
GluI GCNGC 1 cut(s) 39
HaeIII GGCC 1 cut(s) 15
HapII CCGG 1 cut(s) 95
HgaI GACGC 2 cut(s) 120, 193
HhaI GCGC 1 cut(s) 199
Hin1I GRCGYC 2 cut(s) 62, 131
Hin1II CATG 1 cut(s) 52
Hin6I GCGC 1 cut(s) 197
HinP1I GCGC 1 cut(s) 197
HinfI GANTC 1 cut(s) 159
HpaII CCGG 1 cut(s) 95
HphI GGTGA 1 cut(s) 202
Hpy188I TCNGA 2 cut(s) 57, 136
Hpy99I CGWCG 5 cut(s) 64, 67, 103, 136, 167
HpyCH4III ACNGT 1 cut(s) 167
HpyCH4IV ACGT 1 cut(s) 62
HpySE526I ACGT 1 cut(s) 62
Hsp92I GRCGYC 2 cut(s) 62, 131
Hsp92II CATG 1 cut(s) 52
HspAI GCGC 1 cut(s) 197
LpnPI CCDG 5 cut(s) 5, 65, 92, 108, 173
LweI GCATC 1 cut(s) 35
MaeII ACGT 1 cut(s) 62
MaeIII GTNAC 1 cut(s) 190
MboII GAAGA 3 cut(s) 60, 81, 115
MhlI GDGCHC 2 cut(s) 11, 17
MluCI AATT 1 cut(s) 118
MmeI TCCRAC 2 cut(s) 99, 114
MnlI CCTC 3 cut(s) 27, 52, 165
MspI CCGG 1 cut(s) 95
MspR9I CCNGG 2 cut(s) 80, 95
MvaI CCWGG 1 cut(s) 80
NciI CCSGG 1 cut(s) 95
NcoI CCATGG 1 cut(s) 48
NlaIII CATG 1 cut(s) 52
NlaIV GGNNCC 1 cut(s) 15
NmeAIII GCCGAG 1 cut(s) 180
NmuCI GTSAC 1 cut(s) 190
PcsI WCGNNNNNNNCGW 2 cut(s) 68, 128
PfeI GAWTC 1 cut(s) 159
PflFI GACNNNGTC 2 cut(s) 61, 64
PkrI GCNGC 1 cut(s) 40
Psp6I CCWGG 1 cut(s) 78
PspGI CCWGG 1 cut(s) 78
PspN4I GGNNCC 1 cut(s) 15
PspOMI GGGCCC 1 cut(s) 13
PspPI GGNCC 2 cut(s) 13, 14
PsyI GACNNNGTC 2 cut(s) 61, 64
SatI GCNGC 1 cut(s) 39
Sau96I GGNCC 2 cut(s) 13, 14
ScrFI CCNGG 2 cut(s) 80, 95
SduI GDGCHC 2 cut(s) 11, 17
SetI ASST 2 cut(s) 65, 176
SfaNI GCATC 1 cut(s) 35
Sse9I AATT 1 cut(s) 118
SsiI CCGC 2 cut(s) 39, 111
StyD4I CCNGG 2 cut(s) 78, 93
StyI CCWWGG 1 cut(s) 48
TaaI ACNGT 1 cut(s) 167
TaiI ACGT 1 cut(s) 65
TaqI TCGA 2 cut(s) 150, 162
TasI AATT 1 cut(s) 118
TauI GCSGC 1 cut(s) 41
TfiI GAWTC 1 cut(s) 159
TseFI GTSAC 1 cut(s) 190
Tsp45I GTSAC 1 cut(s) 190
TspDTI ATGAA 2 cut(s) 17, 166
Tth111I GACNNNGTC 2 cut(s) 61, 64
XapI RAATTY 1 cut(s) 118
ZraI GACGTC 1 cut(s) 63
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.