Rorug06G0482800

START domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0003829
Physical Location & Seq
Forward (+)
60341324 .. 60351325
10002 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0482800.1

Sequence Viewer

Length: 1902 bp
ATGGTAGCTGCTGTTTCGACGACATTGAACCCCAAAGCCGCCGCCTCCGGTCGTCCTCAGAACGGTACCAGGCCTCCGCTACTACCCTCCGACCCCGAAAATGGTGTCGTTCCTCGCCGCCTCAAGTCTCGAGAAGTCACGTCCCGCTACATGTCGTCTTCTTCCTCCGCCTCCGCGGCTTCGTCTACTTGTGCCAATTCGAAACGGTGCCCCTCGCCGGCGGTCTCAAGGACCGGGGCGGCGAGTCCGATTCCGACGCCGCAGACGCTGAGGCGGTCGCAGTCGGTGGAGAGGAGGAGACCAGTCACTCCGAGACCTAATTCTTTGGATTTGAGGGCCGGGAGTGGAGCTGCCGGCGGCGGTGAAATGTCGGCGGCCCAGAAGCTGCTGGTCACTTCGACGAGGAGTCTATCGGTTTCGTTTCAGGGCGAGTCGTACTCGCTCCAGGTGAGTAAAGCGAAGCGAGCGCCGTCTCCGACTGCGAGGAAAGGCACGCCGGAGCGGAGGAAGGCTTCGACGCCGGCGAGGCCGGATCAGAGCGAGAATTCGAGGCCGATGGAGCAGTACCGGTGGCCGGCGAGGTTGAGGCCGGACACTTGTATGACCAGGAGCTTGGATTGTACGGTGGAGAGGAAGAAAGTGAATGGATCTGGTTCCAATGTGGTTAGGGCATTGCAAAATTCGCTGGGGAATGATCTTGATGGGAAACTGAGGTCTAGTTCGTGCAATTTGGGTTCTGTGAAAGCTACTGAGCCTGTTGATGAAGCTAGAGTTACACAGTCTGAAGCTGTGGCATCCTCTGATACTGACAGTGTGTCTTCTGGTAGTTCCAATTCGGGGCCTCACGAGCTCGGTGGAGGTGGCACTAATTGCTCTGCTGCTGTTGGACAAGCGCCACGTCCTAGAGGGATAGTGGTTCAGGCGAGGTTTTGGCAAGAGACTAACAATCTCTTGCGCAGACAGTCCGAGTATAAGGCTAATGGAACAAGAACTGTGGCCTCTCCGAAGGTTAACGAGGCCAATAGATTATCTATTGATAGTCCTGTGTCATCTCCTCGGGGTGTTTTGAATAGCAGAGGCCAGTTATCCCCAAGTCGTGGAGCTGCTAGGCCTGCATCGCCAAGTAAGCTCAGCCGGCCATCTGTATCATCCCCTTTGAGGGGAGTGAGCCCTTCTCGAGTAAGGAATGGAGTGTCAGCTACGCCAAGTAGTAACTTGAGCAACATGCCTTCGGTTTTAAGTTTTGCTGCTGATGTTCGAAGAGGGAAGGTTGGGGAGAATCGGATAGTTGATGCACATGTGGTGAGGCTCTTACATAACCGGCTGTTGCAGTGGCGTTTTGTGAATGCTAGAGCTAATGCTGCACTAGCCGCACAGAGATCCAATGCAGAGAGAAGTTTATACAATGCGTGGGTAACAACTTCAAAACTCCGGGAATCTGTTAGAGCCAAAAGAACTGAGTTACAACTGCTGAGGCAAAATTTGAAGCTAACTTCCATCCTCCAGGGGCAAATGATATATTTGGAAGAGTGTTCACTTATGGATCGGGATTACTCTAATTCCTTGTCTGGTGCCATCGAAGCTTTGAGGGCTAGCACACTCCGTCTTCCAGTGGTTGATGGAGCAAGGGCAGATGTCCACAATGTGAAGGATGCTATTTCTTCAGCAGTTGATGTGATGCAGGCAATGGCATCATCAATATGCTTATTATTATCGAAGGTTGGGGAGGTGAACTCTTTGGCGGATGAACTTGCAAATGTAAGTGTCAAGGAGCATGCTTTGCTTGGTCAATGCAGGGATCTATTGTCAACAGTTGCAGCCATGCAGGTTGAGGAATGTAGCCTGAGAACACATATTGTACAACAAGAACGTGTACCTTCAAGCCTCACAGCAGAAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
START

Protein Analysis

633

Amino Acids

67.59

Weight (kDa)

10.57

Isoelectric Point (pI)

61.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
QWRF PF04484 271 - 595 6.1e-107 QWRF family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 956
Acc36I ACCTGC 1 cut(s) 1816
Acc65I GGTACC 1 cut(s) 65
AccB1I GGYRCC 3 cut(s) 65, 207, 1571
AccB7I CCANNNNNTGG 1 cut(s) 1097
AccBSI CCGCTC 1 cut(s) 502
AccI GTMKAC 1 cut(s) 185
AccII CGCG 1 cut(s) 176
AclWI GGATC 5 cut(s) 540, 655, 1374, 1551, 1806
AcoI YGGCCR 2 cut(s) 572, 1136
AcsI RAATTY 3 cut(s) 544, 679, 1480
AcuI CTGAAG 2 cut(s) 804, 1647
AcyI GRCGYC 2 cut(s) 257, 518
AdeI CACNNNGTG 1 cut(s) 1645
AfaI GTAC 6 cut(s) 67, 437, 566, 622, 1860, 1875
AfiI CCNNNNNNNGG 9 cut(s) 62, 101, 217, 574, 837, 1097, 1158, 1159, 1160
AflIII ACRYGT 3 cut(s) 150, 1297, 1870
AgeI ACCGGT 1 cut(s) 567
AgsI TTSAA 5 cut(s) 28, 1069, 1425, 1486, 1881
AhdI GACNNNNNGTC 2 cut(s) 405, 814
AjiI CACGTC 2 cut(s) 141, 899
AjnI CCWGG 4 cut(s) 68, 444, 605, 1503
Alw21I GWGCWC 1 cut(s) 852
Alw26I GTCTC 6 cut(s) 132, 229, 292, 307, 477, 932
AlwI GGATC 5 cut(s) 540, 655, 1374, 1551, 1806
AlwNI CAGNNNCTG 2 cut(s) 268, 385
Ama87I CYCGRG 3 cut(s) 129, 1056, 1176
ApeKI GCWGC 8 cut(s) 8, 350, 385, 878, 1103, 1247, 1361, 1817
ApoI RAATTY 3 cut(s) 544, 679, 1480
ArsI GACNNNNNNTTYG 2 cut(s) 90, 122
AsiGI ACCGGT 1 cut(s) 567
Asp718I GGTACC 1 cut(s) 65
AspLEI GCGC 3 cut(s) 469, 895, 957
AspS9I GGNCC 4 cut(s) 231, 336, 376, 839
AsuC2I CCSGG 3 cut(s) 235, 340, 1433
AsuHPI GGTGA 4 cut(s) 374, 460, 1315, 1741
AsuII TTCGAA 2 cut(s) 200, 1258
AsuNHI GCTAGC 1 cut(s) 1592
AvaI CYCGRG 3 cut(s) 129, 1056, 1176
AvaII GGWCC 1 cut(s) 231
BaeGI GKGCMC 1 cut(s) 212
BanI GGYRCC 3 cut(s) 65, 207, 1571
BanII GRGCYC 2 cut(s) 852, 1172
BauI CACGAG 1 cut(s) 845
BbsI GAAGAC 3 cut(s) 150, 810, 1598
Bbv12I GWGCWC 1 cut(s) 852
BbvCI CCTCAGC 2 cut(s) 269, 1472
BbvI GCAGC 7 cut(s) 337, 372, 865, 1090, 1234, 1348, 1829
BccI CCATC 6 cut(s) 550, 695, 1147, 1505, 1583, 1613
BceAI ACGGC 1 cut(s) 454
BciT130I CCWGG 4 cut(s) 70, 446, 607, 1505
BcnI CCSGG 3 cut(s) 235, 340, 1433
BcoDI GTCTC 6 cut(s) 132, 229, 292, 307, 477, 932
BfaI CTAG 7 cut(s) 717, 768, 903, 1107, 1350, 1367, 1593
BfoI RGCGCY 2 cut(s) 470, 896
BfuAI ACCTGC 1 cut(s) 1816
BglI GCCNNNNNGGC 2 cut(s) 176, 526
BlpI GCTNAGC 1 cut(s) 1130
Bme1390I CCNGG 7 cut(s) 70, 235, 340, 446, 607, 1433, 1505
Bme18I GGWCC 1 cut(s) 231
BmeRI GACNNNNNGTC 2 cut(s) 405, 814
BmeT110I CYCGRG 3 cut(s) 129, 1056, 1176
BmgBI CACGTC 2 cut(s) 141, 899
BmgT120I GGNCC 4 cut(s) 231, 336, 376, 839
BmiI GGNNCC 5 cut(s) 67, 209, 655, 840, 1573
BmrFI CCNGG 7 cut(s) 70, 235, 340, 446, 607, 1433, 1505
BmsI GCATC 6 cut(s) 803, 1124, 1282, 1642, 1668, 1700
BmtI GCTAGC 1 cut(s) 1596
BpiI GAAGAC 3 cut(s) 150, 810, 1598
BplI GAGNNNNNCTC 6 cut(s) 422, 454, 1159, 1191, 1718, 1750
BpmI CTGGAG 2 cut(s) 428, 1487
Bpu10I CCTNAGC 2 cut(s) 269, 1472
Bpu1102I GCTNAGC 1 cut(s) 1130
Bpu14I TTCGAA 2 cut(s) 200, 1258
BpuEI CTTGAG 3 cut(s) 107, 211, 1237
BpuMI CCSGG 3 cut(s) 235, 340, 1433
BsaHI GRCGYC 2 cut(s) 257, 518
BsaI GGTCTC 3 cut(s) 229, 292, 307
BsaJI CCNNGG 4 cut(s) 174, 234, 1055, 1504
BsaWI WCCGGW 2 cut(s) 47, 567
BsaXI ACNNNNNCTCC 2 cut(s) 58, 88
Bsc4I CCNNNNNNNGG 9 cut(s) 62, 101, 217, 574, 837, 1097, 1158, 1159, 1160
Bse118I RCCGGY 7 cut(s) 217, 353, 520, 567, 574, 1134, 1320
Bse1I ACTGG 3 cut(s) 302, 1081, 1610
Bse3DI GCAATG 2 cut(s) 671, 1692
BseBI CCWGG 4 cut(s) 70, 446, 607, 1505
BseDI CCNNGG 4 cut(s) 174, 234, 1055, 1504
BseGI GGATG 5 cut(s) 794, 1148, 1497, 1657, 1750
BseLI CCNNNNNNNGG 9 cut(s) 62, 101, 217, 574, 837, 1097, 1158, 1159, 1160
BseMI GCAATG 2 cut(s) 671, 1692
BseMII CTCAG 8 cut(s) 71, 260, 701, 741, 1144, 1449, 1463, 1835
BseNI ACTGG 3 cut(s) 302, 1081, 1610
BseRI GAGGAG 4 cut(s) 307, 310, 418, 1044
BseSI GKGCMC 1 cut(s) 212
BseXI GCAGC 7 cut(s) 337, 372, 865, 1090, 1234, 1348, 1829
BseYI CCCAGC 1 cut(s) 685
BsgI GTGCAG 1 cut(s) 1347
Bsh1236I CGCG 1 cut(s) 176
Bsh1285I CGRYCG 2 cut(s) 52, 278
BshNI GGYRCC 3 cut(s) 65, 207, 1571
BshTI ACCGGT 1 cut(s) 567
BsiEI CGRYCG 2 cut(s) 52, 278
BsiHKAI GWGCWC 1 cut(s) 852
BsiHKCI CYCGRG 3 cut(s) 129, 1056, 1176
BslFI GGGAC 1 cut(s) 127
BslI CCNNNNNNNGG 9 cut(s) 62, 101, 217, 574, 837, 1097, 1158, 1159, 1160
BsmAI GTCTC 6 cut(s) 132, 229, 292, 307, 477, 932
BsmBI CGTCTC 1 cut(s) 477
BsmFI GGGAC 1 cut(s) 127
BsmI GAATGC 1 cut(s) 1351
Bso31I GGTCTC 3 cut(s) 229, 292, 307
BsoBI CYCGRG 3 cut(s) 129, 1056, 1176
Bsp119I TTCGAA 2 cut(s) 200, 1258
Bsp1286I GDGCHC 3 cut(s) 212, 852, 1172
Bsp1407I TGTACA 1 cut(s) 1858
Bsp143I GATC 6 cut(s) 532, 647, 694, 1379, 1543, 1798
Bsp1720I GCTNAGC 1 cut(s) 1130
BspCNI CTCAG 8 cut(s) 70, 261, 702, 742, 1143, 1450, 1464, 1836
BspFNI CGCG 1 cut(s) 176
BspLI GGNNCC 5 cut(s) 67, 209, 655, 840, 1573
BspMI ACCTGC 1 cut(s) 1816
BspOI GCTAGC 1 cut(s) 1596
BspPI GGATC 5 cut(s) 540, 655, 1374, 1551, 1806
BspT104I TTCGAA 2 cut(s) 200, 1258
BspT107I GGYRCC 3 cut(s) 65, 207, 1571
BspTNI GGTCTC 3 cut(s) 229, 292, 307
BsrBI CCGCTC 1 cut(s) 502
BsrDI GCAATG 2 cut(s) 671, 1692
BsrFI RCCGGY 7 cut(s) 217, 353, 520, 567, 574, 1134, 1320
BsrGI TGTACA 1 cut(s) 1858
BsrI ACTGG 3 cut(s) 302, 1081, 1610
BssAI RCCGGY 7 cut(s) 217, 353, 520, 567, 574, 1134, 1320
BssECI CCNNGG 4 cut(s) 174, 234, 1055, 1504
BssMI GATC 6 cut(s) 532, 647, 694, 1379, 1543, 1798
BssNI GRCGYC 2 cut(s) 257, 518
BssSI CACGAG 1 cut(s) 845
Bst2BI CACGAG 1 cut(s) 845
Bst2UI CCWGG 4 cut(s) 70, 446, 607, 1505
Bst4CI ACNGT 8 cut(s) 65, 207, 625, 780, 812, 963, 994, 1813
Bst6I CTCTTC 2 cut(s) 1255, 1521
BstACI GRCGYC 2 cut(s) 257, 518
BstAPI GCANNNNNTGC 2 cut(s) 870, 1780
BstAUI TGTACA 1 cut(s) 1858
BstBI TTCGAA 2 cut(s) 200, 1258
BstDEI CTNAG 8 cut(s) 57, 269, 710, 750, 1130, 1458, 1472, 1844
BstDSI CCRYGG 1 cut(s) 174
BstF5I GGATG 5 cut(s) 794, 1148, 1497, 1657, 1750
BstFNI CGCG 1 cut(s) 176
BstH2I RGCGCY 2 cut(s) 470, 896
BstHHI GCGC 3 cut(s) 469, 895, 957
BstKTI GATC 6 cut(s) 535, 650, 697, 1382, 1546, 1801
BstMAI GTCTC 6 cut(s) 132, 229, 292, 307, 477, 932
BstMBI GATC 6 cut(s) 532, 647, 694, 1379, 1543, 1798
BstMCI CGRYCG 2 cut(s) 52, 278
BstNI CCWGG 4 cut(s) 70, 446, 607, 1505
BstNSI RCATGY 4 cut(s) 154, 1228, 1301, 1778
BstSCI CCNGG 7 cut(s) 68, 233, 338, 444, 605, 1431, 1503
BstSLI GKGCMC 1 cut(s) 212
BstUI CGCG 1 cut(s) 176
BstV1I GCAGC 7 cut(s) 337, 372, 865, 1090, 1234, 1348, 1829
BstV2I GAAGAC 3 cut(s) 150, 810, 1598
BstX2I RGATCY 3 cut(s) 647, 1379, 1798
BstXI CCANNNNNNTGG 1 cut(s) 613
BstYI RGATCY 3 cut(s) 647, 1379, 1798
BtgI CCRYGG 1 cut(s) 174
BtgZI GCGATG 1 cut(s) 1101
BtrI CACGTC 2 cut(s) 141, 899
BtsCI GGATG 5 cut(s) 794, 1148, 1497, 1657, 1750
BtsI GCAGTG 1 cut(s) 1337
BtsIMutI CAGTG 3 cut(s) 817, 1337, 1617
BveI ACCTGC 1 cut(s) 1816
CaiI CAGNNNCTG 2 cut(s) 268, 385
CfoI GCGC 3 cut(s) 469, 895, 957
Cfr10I RCCGGY 7 cut(s) 217, 353, 520, 567, 574, 1134, 1320
Cfr13I GGNCC 4 cut(s) 231, 336, 376, 839
Cfr42I CCGCGG 1 cut(s) 177
CseI GACGC 3 cut(s) 265, 274, 526
Csp6I GTAC 6 cut(s) 66, 436, 565, 621, 1859, 1874
CspAI ACCGGT 1 cut(s) 567
CspCI CAANNNNNGTGG 2 cut(s) 975, 1010
CviAII CATG 5 cut(s) 151, 1225, 1298, 1775, 1822
CviQI GTAC 6 cut(s) 66, 436, 565, 621, 1859, 1874
DdeI CTNAG 8 cut(s) 57, 269, 710, 750, 1130, 1458, 1472, 1844
DpnI GATC 6 cut(s) 534, 649, 696, 1381, 1545, 1800
DpnII GATC 6 cut(s) 532, 647, 694, 1379, 1543, 1798
DraIII CACNNNGTG 1 cut(s) 1645
DriI GACNNNNNGTC 2 cut(s) 405, 814
EaeI YGGCCR 2 cut(s) 572, 1136
Eam1104I CTCTTC 2 cut(s) 1255, 1521
Eam1105I GACNNNNNGTC 2 cut(s) 405, 814
EarI CTCTTC 2 cut(s) 1255, 1521
EciI GGCGGA 2 cut(s) 157, 1757
Ecl136II GAGCTC 1 cut(s) 850
Eco147I AGGCCT 2 cut(s) 73, 1111
Eco24I GRGCYC 2 cut(s) 852, 1172
Eco31I GGTCTC 3 cut(s) 229, 292, 307
Eco47I GGWCC 1 cut(s) 231
Eco53kI GAGCTC 1 cut(s) 850
Eco57I CTGAAG 2 cut(s) 804, 1647
Eco88I CYCGRG 3 cut(s) 129, 1056, 1176
EcoICRI GAGCTC 1 cut(s) 850
EcoO109I RGGNCCY 1 cut(s) 839
EcoRI GAATTC 1 cut(s) 544
EcoRII CCWGG 4 cut(s) 68, 444, 605, 1503
EcoT38I GRGCYC 2 cut(s) 852, 1172
Esp3I CGTCTC 1 cut(s) 477
FaeI CATG 5 cut(s) 154, 1228, 1301, 1778, 1825
FaqI GGGAC 1 cut(s) 127
FatI CATG 5 cut(s) 150, 1224, 1297, 1774, 1821
FauI CCCGC 1 cut(s) 152
FblI GTMKAC 1 cut(s) 185
FokI GGATG 5 cut(s) 781, 1135, 1484, 1664, 1757
FriOI GRGCYC 2 cut(s) 852, 1172
FspBI CTAG 7 cut(s) 717, 768, 903, 1107, 1350, 1367, 1593
FspI TGCGCA 1 cut(s) 956
GlaI GCGC 3 cut(s) 468, 894, 956
GsaI CCCAGC 1 cut(s) 689
GsuI CTGGAG 2 cut(s) 428, 1487
HaeII RGCGCY 2 cut(s) 470, 896
HgaI GACGC 3 cut(s) 265, 274, 526
HhaI GCGC 3 cut(s) 469, 895, 957
Hin1I GRCGYC 2 cut(s) 257, 518
Hin1II CATG 5 cut(s) 154, 1228, 1301, 1778, 1825
Hin6I GCGC 3 cut(s) 467, 893, 955
HinP1I GCGC 3 cut(s) 467, 893, 955
HincII GTYRAC 2 cut(s) 1012, 1809
HindII GTYRAC 2 cut(s) 1012, 1809
HindIII AAGCTT 1 cut(s) 1581
HinfI GANTC 6 cut(s) 244, 250, 406, 431, 1279, 1436
HpaI GTTAAC 1 cut(s) 1012
HphI GGTGA 4 cut(s) 374, 460, 1315, 1741
Hpy166II GTNNAC 7 cut(s) 186, 1012, 1535, 1639, 1732, 1809, 1874
Hpy188III TCNNGA 6 cut(s) 129, 131, 698, 845, 1176, 1547
Hpy8I GTNNAC 7 cut(s) 186, 1012, 1535, 1639, 1732, 1809, 1874
Hpy99I CGWCG 4 cut(s) 22, 259, 403, 520
HpyAV CCTTC 8 cut(s) 502, 1000, 1182, 1239, 1261, 1642, 1711, 1887
HpyCH4III ACNGT 8 cut(s) 65, 207, 625, 780, 812, 963, 994, 1813
HpyCH4IV ACGT 3 cut(s) 140, 898, 1870
HpyF3I CTNAG 8 cut(s) 57, 269, 710, 750, 1130, 1458, 1472, 1844
HpySE526I ACGT 3 cut(s) 140, 898, 1870
Hsp92I GRCGYC 2 cut(s) 257, 518
Hsp92II CATG 5 cut(s) 154, 1228, 1301, 1778, 1825
HspAI GCGC 3 cut(s) 467, 893, 955
KpnI GGTACC 1 cut(s) 69
KroI GCCGGC 5 cut(s) 217, 353, 520, 574, 1134
KroNI GCCGGC 5 cut(s) 219, 355, 522, 576, 1136
KspAI GTTAAC 1 cut(s) 1012
KspI CCGCGG 1 cut(s) 177
Kzo9I GATC 6 cut(s) 532, 647, 694, 1379, 1543, 1798
LmnI GCTCC 8 cut(s) 347, 447, 499, 559, 609, 1100, 1622, 1771
Lsp1109I GCAGC 7 cut(s) 337, 372, 865, 1090, 1234, 1348, 1829
LweI GCATC 6 cut(s) 803, 1124, 1282, 1642, 1668, 1700
MaeI CTAG 7 cut(s) 717, 768, 903, 1107, 1350, 1367, 1593
MaeII ACGT 3 cut(s) 140, 898, 1870
MaeIII GTNAC 7 cut(s) 136, 304, 391, 772, 1211, 1414, 1461
MalI GATC 6 cut(s) 534, 649, 696, 1381, 1545, 1800
MbiI CCGCTC 1 cut(s) 502
MboI GATC 6 cut(s) 532, 647, 694, 1379, 1543, 1798
MboII GAAGA 8 cut(s) 150, 153, 646, 810, 1272, 1538, 1598, 1653
MflI RGATCY 3 cut(s) 647, 1379, 1798
MhlI GDGCHC 3 cut(s) 212, 852, 1172
MluCI AATT 9 cut(s) 196, 319, 544, 679, 727, 832, 868, 1480, 1558
MlyI GAGTC 3 cut(s) 253, 415, 440
MmeI TCCRAC 4 cut(s) 114, 278, 500, 865
MreI CGCCGGCG 2 cut(s) 217, 520
MroNI GCCGGC 5 cut(s) 217, 353, 520, 574, 1134
MseI TTAA 2 cut(s) 1011, 1238
MslI CAYNNNNRTG 2 cut(s) 599, 1699
MspA1I CMGCKG 1 cut(s) 176
MspR9I CCNGG 7 cut(s) 70, 235, 340, 446, 607, 1433, 1505
Mva1269I GAATGC 1 cut(s) 1351
MvaI CCWGG 4 cut(s) 70, 446, 607, 1505
MvnI CGCG 1 cut(s) 176
NaeI GCCGGC 5 cut(s) 219, 355, 522, 576, 1136
NciI CCSGG 3 cut(s) 235, 340, 1433
NdeII GATC 6 cut(s) 532, 647, 694, 1379, 1543, 1798
NgoMIV GCCGGC 5 cut(s) 217, 353, 520, 574, 1134
NheI GCTAGC 1 cut(s) 1592
NlaIII CATG 5 cut(s) 154, 1228, 1301, 1778, 1825
NlaIV GGNNCC 5 cut(s) 67, 209, 655, 840, 1573
NmuCI GTSAC 3 cut(s) 136, 304, 391
NsbI TGCGCA 1 cut(s) 956
NspI RCATGY 4 cut(s) 154, 1228, 1301, 1778
NspV TTCGAA 2 cut(s) 200, 1258
PaeI GCATGC 1 cut(s) 1778
PaeR7I CTCGAG 2 cut(s) 129, 1176
PceI AGGCCT 2 cut(s) 73, 1111
PciI ACATGT 2 cut(s) 150, 1297
PcsI WCGNNNNNNNCGW 1 cut(s) 521
PctI GAATGC 1 cut(s) 1351
PdiI GCCGGC 5 cut(s) 219, 355, 522, 576, 1136
PfeI GAWTC 3 cut(s) 250, 1279, 1436
PflMI CCANNNNNTGG 1 cut(s) 1097
PfoI TCCNGGA 1 cut(s) 1431
PinAI ACCGGT 1 cut(s) 567
PleI GAGTC 3 cut(s) 252, 414, 439
PpsI GAGTC 3 cut(s) 252, 414, 439
PscI ACATGT 2 cut(s) 150, 1297
Psp124BI GAGCTC 1 cut(s) 852
Psp6I CCWGG 4 cut(s) 68, 444, 605, 1503
PspFI CCCAGC 1 cut(s) 685
PspGI CCWGG 4 cut(s) 68, 444, 605, 1503
PspN4I GGNNCC 5 cut(s) 67, 209, 655, 840, 1573
PspPI GGNCC 4 cut(s) 231, 336, 376, 839
PstNI CAGNNNCTG 2 cut(s) 268, 385
PsuI RGATCY 3 cut(s) 647, 1379, 1798
RsaI GTAC 6 cut(s) 67, 437, 566, 622, 1860, 1875
RsaNI GTAC 6 cut(s) 66, 436, 565, 621, 1859, 1874
RseI CAYNNNNRTG 2 cut(s) 599, 1699
SacI GAGCTC 1 cut(s) 852
SacII CCGCGG 1 cut(s) 177
SaqAI TTAA 2 cut(s) 1011, 1238
Sau3AI GATC 6 cut(s) 532, 647, 694, 1379, 1543, 1798
Sau96I GGNCC 4 cut(s) 231, 336, 376, 839
SchI GAGTC 3 cut(s) 253, 415, 440
ScrFI CCNGG 7 cut(s) 70, 235, 340, 446, 607, 1433, 1505
SduI GDGCHC 3 cut(s) 212, 852, 1172
SfaNI GCATC 6 cut(s) 803, 1124, 1282, 1642, 1668, 1700
Sfr274I CTCGAG 2 cut(s) 129, 1176
Sfr303I CCGCGG 1 cut(s) 177
SfuI TTCGAA 2 cut(s) 200, 1258
SgrAI CRCCGGYG 2 cut(s) 217, 520
SgrBI CCGCGG 1 cut(s) 177
SinI GGWCC 1 cut(s) 231
SlaI CTCGAG 2 cut(s) 129, 1176
SmiMI CAYNNNNRTG 2 cut(s) 599, 1699
SmlI CTYRAG 5 cut(s) 122, 129, 226, 1176, 1216
SmoI CTYRAG 5 cut(s) 122, 129, 226, 1176, 1216
SphI GCATGC 1 cut(s) 1778
Sse9I AATT 9 cut(s) 196, 319, 544, 679, 727, 832, 868, 1480, 1558
SseBI AGGCCT 2 cut(s) 73, 1111
SspMI CTAG 7 cut(s) 717, 768, 903, 1107, 1350, 1367, 1593
SstI GAGCTC 1 cut(s) 852
StuI AGGCCT 2 cut(s) 73, 1111
StyD4I CCNGG 7 cut(s) 68, 233, 338, 444, 605, 1431, 1503
TaaI ACNGT 8 cut(s) 65, 207, 625, 780, 812, 963, 994, 1813
TaiI ACGT 3 cut(s) 143, 901, 1873
TasI AATT 9 cut(s) 196, 319, 544, 679, 727, 832, 868, 1480, 1558
TatI WGTACW 1 cut(s) 1858
TauI GCSGC 9 cut(s) 41, 44, 120, 179, 242, 262, 360, 377, 1373
TfiI GAWTC 3 cut(s) 250, 1279, 1436
Tru1I TTAA 2 cut(s) 1011, 1238
Tru9I TTAA 2 cut(s) 1011, 1238
TscAI CASTG 3 cut(s) 817, 1337, 1617
TseFI GTSAC 3 cut(s) 136, 304, 391
TseI GCWGC 8 cut(s) 8, 350, 385, 878, 1103, 1247, 1361, 1817
Tsp45I GTSAC 3 cut(s) 136, 304, 391
TspDTI ATGAA 2 cut(s) 777, 1761
TspGWI ACGGA 1 cut(s) 1592
TspRI CASTG 3 cut(s) 817, 1337, 1617
Van91I CCANNNNNTGG 1 cut(s) 1097
VpaK11BI GGWCC 1 cut(s) 231
XapI RAATTY 3 cut(s) 544, 679, 1480
XceI RCATGY 4 cut(s) 154, 1228, 1301, 1778
XhoI CTCGAG 2 cut(s) 129, 1176
XmiI GTMKAC 1 cut(s) 185
XspI CTAG 7 cut(s) 717, 768, 903, 1107, 1350, 1367, 1593
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.