AT1G64720

START domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0003829
Physical Location & Seq
Reverse (-)
24046553 .. 24048822
2270 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G64720.1

Sequence Viewer

Length: 1158 bp
ATGATGGGATTGAGTTCCTTGGTCTTTACCGAGTTTCACAGCCTCGGCTTCTCTGACCTTATAGTTTTTGTTGCGCCACTCTGGATTGCTGTGGCTGCCGGTGTTTTGGTTGGTTGGGTTTGGAGGCCCAAGTGGGCTTATCTCGATTCTAAACTCTTGTCCAATTCACCTAAATTCCTCAATCTTCAGCTTCCCACTTCCATCCTCAAAACTTCTTCTCACCACGTTAACCCCAGCCTTTCTTCCAAAGAGAAAACTGGGTTTGTAACGGACGATGATTTTAGGCATCTATGGAAGTTGGTTGAGGTCAAAGATGGCGGTCCTTGTTGGATTCAAATGATGGACCGATCAACCCCAACCTTCTCATATCAAGCTTGGAGGCGAGATCCTGAGAATGGGCCTCCCCAGTACCGTAGCAGAACTGTGTTTGAGGATGCCACTCCCGAGATGGTCAGGGACTTCTTTTGGGATGATGAGTTCCGTTCCAAGTGGGATGATATGCTTTTGTATTCTTCTACTCTCGAGCGTTGCAAGGACACGGGCACTATGGTTGTGCAATGGGTGCGAAAGTTCCCCTTCTTTTGTAGCGACAGGGAGTATATTATAGGCCGTCGTATATGGGATGCTGGCCGAGTCTTTTACTGCATTACTAAGGGAGTACAATACCCGTCTGTACCTAGACAAAACAAGCCAAGGCGAGTCGACTTGTACTATTCAAGCTGGTGTATCCGTGCAGTTGAATCAAAAAGAGGCGATGGTGAGATGACATCATGTGAGGTACTACTATTTCACCATGAAGATATGGGAATACCGTGGGAGATTGCAAAGCTTGGGGTGAGACAGGGTATGTGGGGTGCAGTCAAGAAGATTGAGCCAGGCCTACGTGCATATCAGAGGGCTAAAGCAGCGGGAGCAGGTTTATCCCCGAGTGCCATAATGGCTCACATCAACACCAAAGTGAGTGCAGAGGAGTTCATGAATGAGAGGGGTTCGATAGCAGAGGTTACAGGAGACAAGCCGACGGGGAAAAACATACCAAAGATACTAGTGGTAGGAGGGGCGATTGCACTTGCATGTACATTGGACAAAGGGCTGTTGACAAAGGCGGTGATATTCGGAGTAGCCAGAAGATTTGCAAGAATGGGAAAAAGGATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
START

Protein Analysis

385

Amino Acids

43.76

Weight (kDa)

9.33

Isoelectric Point (pI)

40.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
START PF01852 106 - 263 2.9e-06 START domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 905
AccI GTMKAC 1 cut(s) 702
AciI CCGC 3 cut(s) 318, 908, 1106
AclWI GGATC 1 cut(s) 380
AcoI YGGCCR 1 cut(s) 628
AcsI RAATTY 1 cut(s) 173
AcuI CTGAAG 1 cut(s) 170
AfaI GTAC 6 cut(s) 410, 660, 675, 710, 780, 1078
AfiI CCNNNNNNNGG 1 cut(s) 395
AgsI TTSAA 3 cut(s) 335, 717, 740
AhlI ACTAGT 1 cut(s) 1045
AjnI CCWGG 1 cut(s) 874
AleI CACNNNNGTG 1 cut(s) 956
AloI GAACNNNNNNTCC 2 cut(s) 461, 493
AluBI AGCT 4 cut(s) 190, 374, 720, 829
AluI AGCT 4 cut(s) 190, 374, 720, 829
Alw26I GTCTC 2 cut(s) 832, 1005
AlwI GGATC 1 cut(s) 380
Ama87I CYCGRG 3 cut(s) 443, 521, 925
AoxI GGCC 5 cut(s) 125, 398, 607, 628, 877
ApeKI GCWGC 2 cut(s) 95, 905
ApoI RAATTY 1 cut(s) 173
AspLEI GCGC 1 cut(s) 76
AspS9I GGNCC 4 cut(s) 126, 320, 343, 398
AsuHPI GGTGA 6 cut(s) 159, 212, 770, 782, 847, 1120
AvaI CYCGRG 3 cut(s) 443, 521, 925
AvaII GGWCC 2 cut(s) 320, 343
BaeGI GKGCMC 1 cut(s) 545
BbvI GCAGC 2 cut(s) 82, 917
BccI CCATC 5 cut(s) 209, 308, 334, 442, 749
BceAI ACGGC 1 cut(s) 594
BciT130I CCWGG 1 cut(s) 876
BciVI GTATCC 1 cut(s) 737
BcoDI GTCTC 2 cut(s) 832, 1005
BcuI ACTAGT 1 cut(s) 1045
BfaI CTAG 2 cut(s) 678, 1046
BfuAI ACCTGC 1 cut(s) 905
BfuI GTATCC 1 cut(s) 737
BglI GCCNNNNNGGC 1 cut(s) 938
BisI GCNGC 2 cut(s) 96, 906
BlsI GCNGC 2 cut(s) 97, 907
Bme1390I CCNGG 1 cut(s) 876
Bme18I GGWCC 2 cut(s) 320, 343
BmeT110I CYCGRG 3 cut(s) 443, 521, 925
BmgT120I GGNCC 4 cut(s) 126, 320, 343, 398
BmrFI CCNGG 1 cut(s) 876
BmrI ACTGGG 2 cut(s) 267, 400
BmsI GCATC 3 cut(s) 295, 424, 613
BmuI ACTGGG 2 cut(s) 267, 400
BsaAI YACGTR 1 cut(s) 884
BsaJI CCNNGG 4 cut(s) 18, 43, 692, 812
Bsc4I CCNNNNNNNGG 1 cut(s) 395
Bse118I RCCGGY 1 cut(s) 98
Bse1I ACTGG 2 cut(s) 262, 406
Bse3DI GCAATG 1 cut(s) 563
BseBI CCWGG 1 cut(s) 876
BseDI CCNNGG 4 cut(s) 18, 43, 692, 812
BseGI GGATG 6 cut(s) 201, 439, 475, 499, 628, 1158
BseLI CCNNNNNNNGG 1 cut(s) 395
BseMI GCAATG 1 cut(s) 563
BseMII CTCAG 1 cut(s) 381
BseNI ACTGG 2 cut(s) 262, 406
BseRI GAGGAG 1 cut(s) 983
BseSI GKGCMC 1 cut(s) 545
BseXI GCAGC 2 cut(s) 82, 917
BseYI CCCAGC 1 cut(s) 233
BsgI GTGCAG 3 cut(s) 753, 876, 984
BshFI GGCC 5 cut(s) 127, 400, 609, 630, 879
BsiHKCI CYCGRG 3 cut(s) 443, 521, 925
BsiSI CCGG 1 cut(s) 99
BslFI GGGAC 1 cut(s) 470
BslI CCNNNNNNNGG 1 cut(s) 395
BsmAI GTCTC 2 cut(s) 832, 1005
BsmFI GGGAC 1 cut(s) 470
BsnI GGCC 5 cut(s) 127, 400, 609, 630, 879
BsoBI CYCGRG 3 cut(s) 443, 521, 925
Bsp1286I GDGCHC 1 cut(s) 545
Bsp1407I TGTACA 1 cut(s) 1076
Bsp143I GATC 2 cut(s) 347, 385
BspACI CCGC 3 cut(s) 318, 908, 1106
BspANI GGCC 5 cut(s) 127, 400, 609, 630, 879
BspCNI CTCAG 1 cut(s) 382
BspHI TCATGA 1 cut(s) 975
BspMI ACCTGC 1 cut(s) 905
BspPI GGATC 1 cut(s) 380
BsrDI GCAATG 1 cut(s) 563
BsrFI RCCGGY 1 cut(s) 98
BsrGI TGTACA 1 cut(s) 1076
BsrI ACTGG 2 cut(s) 262, 406
BssAI RCCGGY 1 cut(s) 98
BssECI CCNNGG 4 cut(s) 18, 43, 692, 812
BssMI GATC 2 cut(s) 347, 385
BssT1I CCWWGG 2 cut(s) 18, 692
Bst2UI CCWGG 1 cut(s) 876
Bst4CI ACNGT 3 cut(s) 413, 424, 813
BstAPI GCANNNNNTGC 1 cut(s) 562
BstAUI TGTACA 1 cut(s) 1076
BstBAI YACGTR 1 cut(s) 884
BstC8I GCNNGC 1 cut(s) 628
BstDEI CTNAG 2 cut(s) 390, 651
BstDSI CCRYGG 1 cut(s) 812
BstF5I GGATG 6 cut(s) 201, 439, 475, 499, 628, 1158
BstHHI GCGC 1 cut(s) 76
BstKTI GATC 2 cut(s) 350, 388
BstMAI GTCTC 2 cut(s) 832, 1005
BstMBI GATC 2 cut(s) 347, 385
BstMWI GCNNNNNNNGC 5 cut(s) 95, 562, 905, 911, 938
BstNI CCWGG 1 cut(s) 876
BstNSI RCATGY 1 cut(s) 1077
BstSCI CCNGG 1 cut(s) 874
BstSLI GKGCMC 1 cut(s) 545
BstV1I GCAGC 2 cut(s) 82, 917
BstX2I RGATCY 1 cut(s) 385
BstYI RGATCY 1 cut(s) 385
BsuI GTATCC 1 cut(s) 737
BsuRI GGCC 5 cut(s) 127, 400, 609, 630, 879
BtgI CCRYGG 1 cut(s) 812
BtgZI GCGATG 1 cut(s) 768
BtsCI GGATG 6 cut(s) 201, 439, 475, 499, 628, 1158
BveI ACCTGC 1 cut(s) 905
Cac8I GCNNGC 1 cut(s) 628
CciI TCATGA 1 cut(s) 975
CfoI GCGC 1 cut(s) 76
Cfr10I RCCGGY 1 cut(s) 98
Cfr13I GGNCC 4 cut(s) 126, 320, 343, 398
Csp6I GTAC 6 cut(s) 409, 659, 674, 709, 779, 1077
CviAII CATG 4 cut(s) 771, 794, 976, 1074
CviQI GTAC 6 cut(s) 409, 659, 674, 709, 779, 1077
DdeI CTNAG 2 cut(s) 390, 651
DpnI GATC 2 cut(s) 349, 387
DpnII GATC 2 cut(s) 347, 385
EaeI YGGCCR 1 cut(s) 628
Eco130I CCWWGG 2 cut(s) 18, 692
Eco147I AGGCCT 1 cut(s) 879
Eco47I GGWCC 2 cut(s) 320, 343
Eco57I CTGAAG 1 cut(s) 170
Eco88I CYCGRG 3 cut(s) 443, 521, 925
EcoRII CCWGG 1 cut(s) 874
EcoT14I CCWWGG 2 cut(s) 18, 692
ErhI CCWWGG 2 cut(s) 18, 692
FaeI CATG 4 cut(s) 774, 797, 979, 1077
FalI AAGNNNNNCTT 2 cut(s) 560, 592
FaqI GGGAC 1 cut(s) 470
FatI CATG 4 cut(s) 770, 793, 975, 1073
FauI CCCGC 1 cut(s) 901
FblI GTMKAC 1 cut(s) 702
Fnu4HI GCNGC 2 cut(s) 96, 906
FokI GGATG 5 cut(s) 188, 446, 482, 506, 635
Fsp4HI GCNGC 2 cut(s) 96, 906
FspBI CTAG 2 cut(s) 678, 1046
GlaI GCGC 1 cut(s) 75
GluI GCNGC 2 cut(s) 96, 906
GsaI CCCAGC 1 cut(s) 237
HaeIII GGCC 5 cut(s) 127, 400, 609, 630, 879
HapII CCGG 1 cut(s) 99
HhaI GCGC 1 cut(s) 76
Hin1II CATG 4 cut(s) 774, 797, 979, 1077
Hin6I GCGC 1 cut(s) 74
HinP1I GCGC 1 cut(s) 74
HincII GTYRAC 3 cut(s) 229, 703, 1098
HindII GTYRAC 3 cut(s) 229, 703, 1098
HindIII AAGCTT 2 cut(s) 372, 827
HinfI GANTC 5 cut(s) 146, 331, 633, 699, 740
HpaI GTTAAC 1 cut(s) 229
HpaII CCGG 1 cut(s) 99
HphI GGTGA 6 cut(s) 159, 212, 770, 782, 847, 1120
Hpy166II GTNNAC 3 cut(s) 229, 703, 1098
Hpy188I TCNGA 3 cut(s) 55, 894, 1118
Hpy188III TCNNGA 7 cut(s) 82, 143, 389, 443, 521, 862, 976
Hpy8I GTNNAC 3 cut(s) 229, 703, 1098
Hpy99I CGWCG 2 cut(s) 615, 1024
HpyAV CCTTC 2 cut(s) 370, 586
HpyCH4III ACNGT 3 cut(s) 413, 424, 813
HpyCH4IV ACGT 2 cut(s) 225, 883
HpyF10VI GCNNNNNNNGC 5 cut(s) 95, 562, 905, 911, 938
HpyF3I CTNAG 2 cut(s) 390, 651
HpySE526I ACGT 2 cut(s) 225, 883
Hsp92II CATG 4 cut(s) 774, 797, 979, 1077
HspAI GCGC 1 cut(s) 74
KspAI GTTAAC 1 cut(s) 229
Kzo9I GATC 2 cut(s) 347, 385
LmnI GCTCC 1 cut(s) 911
Lsp1109I GCAGC 2 cut(s) 82, 917
LweI GCATC 3 cut(s) 295, 424, 613
MaeI CTAG 2 cut(s) 678, 1046
MaeII ACGT 2 cut(s) 225, 883
MaeIII GTNAC 2 cut(s) 265, 1003
MalI GATC 2 cut(s) 349, 387
MboI GATC 2 cut(s) 347, 385
MboII GAAGA 7 cut(s) 176, 207, 234, 504, 809, 877, 1140
MflI RGATCY 1 cut(s) 385
MhlI GDGCHC 1 cut(s) 545
MluCI AATT 2 cut(s) 163, 173
MlyI GAGTC 2 cut(s) 642, 708
MmeI TCCRAC 1 cut(s) 308
MseI TTAA 1 cut(s) 228
MslI CAYNNNNRTG 2 cut(s) 956, 1072
MspA1I CMGCKG 1 cut(s) 908
MspI CCGG 1 cut(s) 99
MspR9I CCNGG 1 cut(s) 876
MvaI CCWGG 1 cut(s) 876
MwoI GCNNNNNNNGC 5 cut(s) 95, 562, 905, 911, 938
NdeII GATC 2 cut(s) 347, 385
NlaIII CATG 4 cut(s) 774, 797, 979, 1077
NmeAIII GCCGAG 2 cut(s) 24, 656
NspI RCATGY 1 cut(s) 1077
OliI CACNNNNGTG 1 cut(s) 956
PaeR7I CTCGAG 1 cut(s) 521
PagI TCATGA 1 cut(s) 975
PceI AGGCCT 1 cut(s) 879
PfeI GAWTC 3 cut(s) 146, 331, 740
PkrI GCNGC 2 cut(s) 97, 907
PleI GAGTC 2 cut(s) 641, 707
PpsI GAGTC 2 cut(s) 641, 707
Ppu21I YACGTR 1 cut(s) 884
Psp6I CCWGG 1 cut(s) 874
PspFI CCCAGC 1 cut(s) 233
PspGI CCWGG 1 cut(s) 874
PspPI GGNCC 4 cut(s) 126, 320, 343, 398
PsuI RGATCY 1 cut(s) 385
RsaI GTAC 6 cut(s) 410, 660, 675, 710, 780, 1078
RsaNI GTAC 6 cut(s) 409, 659, 674, 709, 779, 1077
RseI CAYNNNNRTG 2 cut(s) 956, 1072
SalI GTCGAC 1 cut(s) 701
SaqAI TTAA 1 cut(s) 228
SatI GCNGC 2 cut(s) 96, 906
Sau3AI GATC 2 cut(s) 347, 385
Sau96I GGNCC 4 cut(s) 126, 320, 343, 398
SchI GAGTC 2 cut(s) 642, 708
ScrFI CCNGG 1 cut(s) 876
SduI GDGCHC 1 cut(s) 545
SfaNI GCATC 3 cut(s) 295, 424, 613
Sfr274I CTCGAG 1 cut(s) 521
SinI GGWCC 2 cut(s) 320, 343
SlaI CTCGAG 1 cut(s) 521
SmiMI CAYNNNNRTG 2 cut(s) 956, 1072
SmlI CTYRAG 1 cut(s) 521
SmoI CTYRAG 1 cut(s) 521
SpeI ACTAGT 1 cut(s) 1045
Sse9I AATT 2 cut(s) 163, 173
SseBI AGGCCT 1 cut(s) 879
SsiI CCGC 3 cut(s) 318, 908, 1106
SspMI CTAG 2 cut(s) 678, 1046
StuI AGGCCT 1 cut(s) 879
StyD4I CCNGG 1 cut(s) 874
StyI CCWWGG 2 cut(s) 18, 692
TaaI ACNGT 3 cut(s) 413, 424, 813
TaiI ACGT 2 cut(s) 228, 886
TaqI TCGA 4 cut(s) 144, 522, 702, 992
TaqII GACCGA 1 cut(s) 360
TasI AATT 2 cut(s) 163, 173
TatI WGTACW 3 cut(s) 658, 708, 1076
TfiI GAWTC 3 cut(s) 146, 331, 740
Tru1I TTAA 1 cut(s) 228
Tru9I TTAA 1 cut(s) 228
TseI GCWGC 2 cut(s) 95, 905
TspDTI ATGAA 3 cut(s) 810, 964, 992
TspGWI ACGGA 3 cut(s) 284, 470, 719
VpaK11BI GGWCC 2 cut(s) 320, 343
XapI RAATTY 1 cut(s) 173
XceI RCATGY 1 cut(s) 1077
XcmI CCANNNNNNNNNTGG 1 cut(s) 445
XhoI CTCGAG 1 cut(s) 521
XmiI GTMKAC 1 cut(s) 702
XspI CTAG 2 cut(s) 678, 1046
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.