FvH4_5g10520

START domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0003829
Physical Location & Seq
Forward (+)
5987830 .. 5991535
3706 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g10520.t1

Sequence Viewer

Length: 1317 bp
ATGGGTTTGGCGTCGGCTTTGGTGGAGATTCTGGGGAGACCGGAAGTGTTCTGGGAGCTGGTGAAGTTCATAACCCCTATATGGATTGCTGTGATTGTTGGGGTTTTCGTTGGGTGGGCGTGGCGGCCCAAATGGGTGACGGTGGTTGGTAGAGAATTGTTGGATAGCTCCTCACAAAAGGAGTCGTCTTCTTCTTCTTCTTCCTCATCGGCTTCTTCATCATCTTCATCGCCACCCGCGCCGGCCACGTGTGGGATTTCGATTTCGAGTTTGAGTTCGTTGCTGCCAAACTGCATTCCTTGGGCTGCTGACGATACCGTTGAGGACCGGGCTCTTACTTTGCCACCTACCACCAATACTGATTCCAGTTGCTCACAGATTGAAAAAGAAGAATCTTGTAGTGTCAACGATGAGGATTTACGCCACATATGTCAGCTTGTTGAGGAGAAAGATGGAGGTCCTGCCTGGATTCATATGATGAATAAGTCAACTCCAACTATGGGCTACCAAGCTTGGCGTAGAGATCCTCAGACTGGACCTCCACAATACCGAAGTAGAACTGTATTTGAAGATGCTTCACCTGAGACAGTGAGGGATTTCTTTTGGGATGATGAGTTTCGAGCAAAGTGGGATGACATGCTTATCCATGCTTCTACTTTGGAAGAATGCCCGACTACTGGAACTATGCTAGTGCACTGGGTCCGCAAGTTTCCATTCTTTTGTAGCGACAGAGATTACATGATAGGCCGTCGCATTTGGGAATCAGGACGATCGTACTACTGTGTAACTAAGGGAGTGCCCTCCTCTGCTGTGCCAAGGCGTGACAAACCTAGACGTGTTGATTTGTACTATTCAAGCTGGTGCATTCGTGCAGTTGAATCAAAAAGAGGGGATGGCCAGCTTACTTCATGTGAGGTTATACTGTTTCACCATGAAGATATGGGTATTCCTTGGGAGATTGCGAAACTTGGAGTTCGTCAGGGTATGTGGGGAGCTGTAAAAAAGATTGAACCAGGCCTACGTTTATATCAGAAGGAGAGAGCCGCTAAAGCGCCACTTTCCCGGTGTGCTAAAATGGCCCAGATCAACACAAAAGTCAGTGCAGATTACTTGAGATCGGTGGAGAACAGCACCAAGGATTCCATGGAGGTTGAAATTTCAGATTCAGCGGAGAAACCAGTAGGGAGGAACATACCTAAAGCTCTAATTGTTGGAGGGGCATTAGTTCTTGCTTGCAGTCTTGATCGGGGGCTCTTGACTAAGGCCGTTATATTTGGGGTAGCCAGAAGATTTGCCAAGATTGGGAGGAGGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
START

Protein Analysis

439

Amino Acids

48.9

Weight (kDa)

8.19

Isoelectric Point (pI)

58.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
START PF01852 154 - 314 8.4e-09 START domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 239
AciI CCGC 5 cut(s) 124, 237, 703, 1044, 1169
AclWI GGATC 1 cut(s) 518
AcoI YGGCCR 2 cut(s) 243, 895
AcsI RAATTY 1 cut(s) 1155
AcvI CACGTG 1 cut(s) 249
AcyI GRCGYC 1 cut(s) 11
AfaI GTAC 2 cut(s) 776, 848
AfiI CCNNNNNNNGG 6 cut(s) 81, 252, 500, 533, 677, 1302
AflIII ACRYGT 2 cut(s) 248, 835
AgsI TTSAA 6 cut(s) 383, 569, 855, 878, 1010, 1154
AjiI CACGTC 1 cut(s) 836
AjnI CCWGG 2 cut(s) 464, 1012
AluBI AGCT 8 cut(s) 58, 168, 436, 512, 858, 901, 995, 1202
AluI AGCT 8 cut(s) 58, 168, 436, 512, 858, 901, 995, 1202
Alw21I GWGCWC 1 cut(s) 696
Alw26I GTCTC 2 cut(s) 31, 578
Alw44I GTGCAC 1 cut(s) 692
AlwI GGATC 1 cut(s) 518
AoxI GGCC 7 cut(s) 125, 243, 745, 895, 1015, 1077, 1263
ApaLI GTGCAC 1 cut(s) 692
ApeKI GCWGC 2 cut(s) 283, 305
ApoI RAATTY 1 cut(s) 1155
AspLEI GCGC 2 cut(s) 241, 1054
AspS9I GGNCC 6 cut(s) 126, 325, 458, 536, 700, 1078
AsuC2I CCSGG 2 cut(s) 329, 1063
AsuHPI GGTGA 4 cut(s) 73, 148, 570, 920
AvaII GGWCC 4 cut(s) 325, 458, 536, 700
BaeGI GKGCMC 2 cut(s) 696, 801
BalI TGGCCA 1 cut(s) 897
BanII GRGCYC 2 cut(s) 334, 1254
BbrPI CACGTG 1 cut(s) 249
BbsI GAAGAC 1 cut(s) 180
Bbv12I GWGCWC 1 cut(s) 696
BbvI GCAGC 2 cut(s) 270, 292
BccI CCATC 2 cut(s) 446, 887
BceAI ACGGC 2 cut(s) 732, 1250
BciT130I CCWGG 2 cut(s) 466, 1014
BcnI CCSGG 2 cut(s) 329, 1063
BcoDI GTCTC 2 cut(s) 31, 578
BfaI CTAG 2 cut(s) 689, 831
BfoI RGCGCY 1 cut(s) 1055
BisI GCNGC 4 cut(s) 125, 284, 306, 1044
BlsI GCNGC 4 cut(s) 126, 285, 307, 1045
Bme1390I CCNGG 4 cut(s) 329, 466, 1014, 1063
Bme18I GGWCC 4 cut(s) 325, 458, 536, 700
BmgBI CACGTC 1 cut(s) 836
BmgT120I GGNCC 6 cut(s) 126, 325, 458, 536, 700, 1078
BmiI GGNNCC 1 cut(s) 701
BmrFI CCNGG 4 cut(s) 329, 466, 1014, 1063
BmrI ACTGGG 1 cut(s) 706
BmsI GCATC 1 cut(s) 562
BmuI ACTGGG 1 cut(s) 706
BpiI GAAGAC 1 cut(s) 180
BpuEI CTTGAG 1 cut(s) 1132
BpuMI CCSGG 2 cut(s) 329, 1063
BsaAI YACGTR 1 cut(s) 249
BsaHI GRCGYC 1 cut(s) 11
BsaI GGTCTC 1 cut(s) 31
BsaJI CCNNGG 5 cut(s) 299, 815, 950, 1134, 1143
BsaWI WCCGGW 1 cut(s) 40
BsaXI ACNNNNNCTCC 2 cut(s) 523, 553
Bsc4I CCNNNNNNNGG 6 cut(s) 81, 252, 500, 533, 677, 1302
Bse118I RCCGGY 1 cut(s) 241
Bse1I ACTGG 5 cut(s) 366, 538, 682, 701, 1178
BseBI CCWGG 2 cut(s) 466, 1014
BseDI CCNNGG 5 cut(s) 299, 815, 950, 1134, 1143
BseGI GGATG 3 cut(s) 613, 637, 898
BseLI CCNNNNNNNGG 6 cut(s) 81, 252, 500, 533, 677, 1302
BseMII CTCAG 2 cut(s) 542, 573
BseNI ACTGG 5 cut(s) 366, 538, 682, 701, 1178
BseRI GAGGAG 3 cut(s) 160, 458, 793
BseSI GKGCMC 2 cut(s) 696, 801
BseXI GCAGC 2 cut(s) 270, 292
BsgI GTGCAG 2 cut(s) 891, 1122
Bsh1236I CGCG 1 cut(s) 239
Bsh1285I CGRYCG 1 cut(s) 773
BshFI GGCC 7 cut(s) 127, 245, 747, 897, 1017, 1079, 1265
BsiEI CGRYCG 1 cut(s) 773
BsiHKAI GWGCWC 1 cut(s) 696
BsiSI CCGG 4 cut(s) 41, 242, 328, 1063
BslI CCNNNNNNNGG 6 cut(s) 81, 252, 500, 533, 677, 1302
BsmAI GTCTC 2 cut(s) 31, 578
BsmI GAATGC 3 cut(s) 294, 671, 864
BsnI GGCC 7 cut(s) 127, 245, 747, 897, 1017, 1079, 1265
Bso31I GGTCTC 1 cut(s) 31
Bsp1286I GDGCHC 4 cut(s) 334, 696, 801, 1254
Bsp143I GATC 5 cut(s) 523, 770, 1083, 1115, 1243
Bsp19I CCATGG 1 cut(s) 1143
BspACI CCGC 5 cut(s) 124, 237, 703, 1044, 1169
BspANI GGCC 7 cut(s) 127, 245, 747, 897, 1017, 1079, 1265
BspCNI CTCAG 2 cut(s) 541, 574
BspFNI CGCG 1 cut(s) 239
BspLI GGNNCC 1 cut(s) 701
BspPI GGATC 1 cut(s) 518
BspTNI GGTCTC 1 cut(s) 31
BsrFI RCCGGY 1 cut(s) 241
BsrI ACTGG 5 cut(s) 366, 538, 682, 701, 1178
BssAI RCCGGY 1 cut(s) 241
BssECI CCNNGG 5 cut(s) 299, 815, 950, 1134, 1143
BssMI GATC 5 cut(s) 523, 770, 1083, 1115, 1243
BssNI GRCGYC 1 cut(s) 11
BssT1I CCWWGG 5 cut(s) 299, 815, 950, 1134, 1143
Bst2UI CCWGG 2 cut(s) 466, 1014
Bst4CI ACNGT 6 cut(s) 142, 319, 562, 589, 782, 924
BstACI GRCGYC 1 cut(s) 11
BstBAI YACGTR 1 cut(s) 249
BstC8I GCNNGC 3 cut(s) 243, 899, 1234
BstDEI CTNAG 4 cut(s) 528, 582, 789, 1260
BstDSI CCRYGG 1 cut(s) 1143
BstF5I GGATG 3 cut(s) 613, 637, 898
BstFNI CGCG 1 cut(s) 239
BstH2I RGCGCY 1 cut(s) 1055
BstHHI GCGC 2 cut(s) 241, 1054
BstKTI GATC 5 cut(s) 526, 773, 1086, 1118, 1246
BstMAI GTCTC 2 cut(s) 31, 578
BstMBI GATC 5 cut(s) 523, 770, 1083, 1115, 1243
BstMCI CGRYCG 1 cut(s) 773
BstMWI GCNNNNNNNGC 3 cut(s) 238, 1049, 1076
BstNI CCWGG 2 cut(s) 466, 1014
BstNSI RCATGY 1 cut(s) 640
BstSCI CCNGG 4 cut(s) 327, 464, 1012, 1061
BstSLI GKGCMC 2 cut(s) 696, 801
BstUI CGCG 1 cut(s) 239
BstV1I GCAGC 2 cut(s) 270, 292
BstV2I GAAGAC 1 cut(s) 180
BstX2I RGATCY 1 cut(s) 523
BstYI RGATCY 1 cut(s) 523
BsuRI GGCC 7 cut(s) 127, 245, 747, 897, 1017, 1079, 1265
BtgI CCRYGG 1 cut(s) 1143
BtgZI GCGATG 1 cut(s) 213
BtrI CACGTC 1 cut(s) 836
BtsCI GGATG 3 cut(s) 613, 637, 898
BtsIMutI CAGTG 3 cut(s) 594, 694, 1105
Cac8I GCNNGC 3 cut(s) 243, 899, 1234
CfoI GCGC 2 cut(s) 241, 1054
Cfr10I RCCGGY 1 cut(s) 241
Cfr13I GGNCC 6 cut(s) 126, 325, 458, 536, 700, 1078
Csp6I GTAC 2 cut(s) 775, 847
CviAII CATG 6 cut(s) 637, 647, 739, 909, 932, 1144
CviQI GTAC 2 cut(s) 775, 847
DdeI CTNAG 4 cut(s) 528, 582, 789, 1260
DpnI GATC 5 cut(s) 525, 772, 1085, 1117, 1245
DpnII GATC 5 cut(s) 523, 770, 1083, 1115, 1243
EaeI YGGCCR 2 cut(s) 243, 895
Eco130I CCWWGG 5 cut(s) 299, 815, 950, 1134, 1143
Eco147I AGGCCT 1 cut(s) 1017
Eco24I GRGCYC 2 cut(s) 334, 1254
Eco31I GGTCTC 1 cut(s) 31
Eco47I GGWCC 4 cut(s) 325, 458, 536, 700
Eco72I CACGTG 1 cut(s) 249
EcoO109I RGGNCCY 1 cut(s) 458
EcoRII CCWGG 2 cut(s) 464, 1012
EcoT14I CCWWGG 5 cut(s) 299, 815, 950, 1134, 1143
EcoT38I GRGCYC 2 cut(s) 334, 1254
ErhI CCWWGG 5 cut(s) 299, 815, 950, 1134, 1143
FaeI CATG 6 cut(s) 640, 650, 742, 912, 935, 1147
FalI AAGNNNNNCTT 2 cut(s) 1041, 1073
FatI CATG 6 cut(s) 636, 646, 738, 908, 931, 1143
FauI CCCGC 1 cut(s) 244
FauNDI CATATG 2 cut(s) 428, 474
Fnu4HI GCNGC 4 cut(s) 125, 284, 306, 1044
FokI GGATG 3 cut(s) 620, 644, 905
FriOI GRGCYC 2 cut(s) 334, 1254
Fsp4HI GCNGC 4 cut(s) 125, 284, 306, 1044
FspBI CTAG 2 cut(s) 689, 831
GlaI GCGC 2 cut(s) 240, 1053
GluI GCNGC 4 cut(s) 125, 284, 306, 1044
HaeII RGCGCY 1 cut(s) 1055
HaeIII GGCC 7 cut(s) 127, 245, 747, 897, 1017, 1079, 1265
HapII CCGG 4 cut(s) 41, 242, 328, 1063
HhaI GCGC 2 cut(s) 241, 1054
Hin1I GRCGYC 1 cut(s) 11
Hin1II CATG 6 cut(s) 640, 650, 742, 912, 935, 1147
Hin6I GCGC 2 cut(s) 239, 1052
HinP1I GCGC 2 cut(s) 239, 1052
HincII GTYRAC 2 cut(s) 406, 489
HindII GTYRAC 2 cut(s) 406, 489
HindIII AAGCTT 1 cut(s) 510
HinfI GANTC 9 cut(s) 28, 182, 362, 392, 469, 761, 878, 1139, 1163
HpaII CCGG 4 cut(s) 41, 242, 328, 1063
HphI GGTGA 4 cut(s) 73, 148, 570, 920
Hpy166II GTNNAC 3 cut(s) 406, 489, 694
Hpy188I TCNGA 3 cut(s) 531, 1032, 1162
Hpy188III TCNNGA 3 cut(s) 765, 1241, 1255
Hpy8I GTNNAC 3 cut(s) 406, 489, 694
Hpy99I CGWCG 2 cut(s) 16, 753
HpyAV CCTTC 1 cut(s) 1027
HpyCH4III ACNGT 6 cut(s) 142, 319, 562, 589, 782, 924
HpyCH4IV ACGT 3 cut(s) 248, 835, 1021
HpyCH4V TGCA 6 cut(s) 294, 694, 864, 872, 1103, 1236
HpyF10VI GCNNNNNNNGC 3 cut(s) 238, 1049, 1076
HpyF3I CTNAG 4 cut(s) 528, 582, 789, 1260
HpySE526I ACGT 3 cut(s) 248, 835, 1021
Hsp92I GRCGYC 1 cut(s) 11
Hsp92II CATG 6 cut(s) 640, 650, 742, 912, 935, 1147
HspAI GCGC 2 cut(s) 239, 1052
KroI GCCGGC 1 cut(s) 241
KroNI GCCGGC 1 cut(s) 243
Kzo9I GATC 5 cut(s) 523, 770, 1083, 1115, 1243
LmnI GCTCC 3 cut(s) 55, 173, 992
Lsp1109I GCAGC 2 cut(s) 270, 292
LweI GCATC 1 cut(s) 562
MaeI CTAG 2 cut(s) 689, 831
MaeII ACGT 3 cut(s) 248, 835, 1021
MaeIII GTNAC 3 cut(s) 136, 784, 821
MalI GATC 5 cut(s) 525, 772, 1085, 1117, 1245
MboI GATC 5 cut(s) 523, 770, 1083, 1115, 1243
MflI RGATCY 1 cut(s) 523
MhlI GDGCHC 4 cut(s) 334, 696, 801, 1254
MlsI TGGCCA 1 cut(s) 897
MluCI AATT 3 cut(s) 155, 1155, 1206
MluNI TGGCCA 1 cut(s) 897
MlyI GAGTC 1 cut(s) 191
MmeI TCCRAC 3 cut(s) 141, 518, 1192
Mox20I TGGCCA 1 cut(s) 897
MroNI GCCGGC 1 cut(s) 241
MscI TGGCCA 1 cut(s) 897
Msp20I TGGCCA 1 cut(s) 897
MspA1I CMGCKG 1 cut(s) 1169
MspI CCGG 4 cut(s) 41, 242, 328, 1063
MspR9I CCNGG 4 cut(s) 329, 466, 1014, 1063
Mva1269I GAATGC 3 cut(s) 294, 671, 864
MvaI CCWGG 2 cut(s) 466, 1014
MvnI CGCG 1 cut(s) 239
MwoI GCNNNNNNNGC 3 cut(s) 238, 1049, 1076
NaeI GCCGGC 1 cut(s) 243
NciI CCSGG 2 cut(s) 329, 1063
NcoI CCATGG 1 cut(s) 1143
NdeI CATATG 2 cut(s) 428, 474
NdeII GATC 5 cut(s) 523, 770, 1083, 1115, 1243
NgoMIV GCCGGC 1 cut(s) 241
NlaIII CATG 6 cut(s) 640, 650, 742, 912, 935, 1147
NlaIV GGNNCC 1 cut(s) 701
NmuCI GTSAC 2 cut(s) 136, 821
NspI RCATGY 1 cut(s) 640
PceI AGGCCT 1 cut(s) 1017
PctI GAATGC 3 cut(s) 294, 671, 864
PdiI GCCGGC 1 cut(s) 243
PfeI GAWTC 8 cut(s) 28, 362, 392, 469, 761, 878, 1139, 1163
PkrI GCNGC 4 cut(s) 126, 285, 307, 1045
Ple19I CGATCG 1 cut(s) 773
PleI GAGTC 1 cut(s) 190
PmaCI CACGTG 1 cut(s) 249
PmlI CACGTG 1 cut(s) 249
PpsI GAGTC 1 cut(s) 190
Ppu21I YACGTR 1 cut(s) 249
PpuMI RGGWCCY 1 cut(s) 458
Psp5II RGGWCCY 1 cut(s) 458
Psp6I CCWGG 2 cut(s) 464, 1012
PspCI CACGTG 1 cut(s) 249
PspGI CCWGG 2 cut(s) 464, 1012
PspN4I GGNNCC 1 cut(s) 701
PspPI GGNCC 6 cut(s) 126, 325, 458, 536, 700, 1078
PspPPI RGGWCCY 1 cut(s) 458
PsrI GAACNNNNNNTAC 2 cut(s) 1002, 1034
PsuI RGATCY 1 cut(s) 523
PvuI CGATCG 1 cut(s) 773
RsaI GTAC 2 cut(s) 776, 848
RsaNI GTAC 2 cut(s) 775, 847
SatI GCNGC 4 cut(s) 125, 284, 306, 1044
Sau3AI GATC 5 cut(s) 523, 770, 1083, 1115, 1243
Sau96I GGNCC 6 cut(s) 126, 325, 458, 536, 700, 1078
SchI GAGTC 1 cut(s) 191
ScrFI CCNGG 4 cut(s) 329, 466, 1014, 1063
SduI GDGCHC 4 cut(s) 334, 696, 801, 1254
SfaNI GCATC 1 cut(s) 562
SinI GGWCC 4 cut(s) 325, 458, 536, 700
SmlI CTYRAG 1 cut(s) 1111
SmoI CTYRAG 1 cut(s) 1111
Sse9I AATT 3 cut(s) 155, 1155, 1206
SseBI AGGCCT 1 cut(s) 1017
SsiI CCGC 5 cut(s) 124, 237, 703, 1044, 1169
SspMI CTAG 2 cut(s) 689, 831
StuI AGGCCT 1 cut(s) 1017
StyD4I CCNGG 4 cut(s) 327, 464, 1012, 1061
StyI CCWWGG 5 cut(s) 299, 815, 950, 1134, 1143
TaaI ACNGT 6 cut(s) 142, 319, 562, 589, 782, 924
TaiI ACGT 3 cut(s) 251, 838, 1024
TaqI TCGA 3 cut(s) 260, 266, 619
TasI AATT 3 cut(s) 155, 1155, 1206
TatI WGTACW 1 cut(s) 846
TauI GCSGC 2 cut(s) 127, 1046
TfiI GAWTC 8 cut(s) 28, 362, 392, 469, 761, 878, 1139, 1163
TscAI CASTG 3 cut(s) 594, 701, 1105
TseFI GTSAC 2 cut(s) 136, 821
TseI GCWGC 2 cut(s) 283, 305
Tsp45I GTSAC 2 cut(s) 136, 821
TspDTI ATGAA 7 cut(s) 58, 207, 216, 461, 494, 897, 948
TspRI CASTG 3 cut(s) 594, 701, 1105
VneI GTGCAC 1 cut(s) 692
VpaK11BI GGWCC 4 cut(s) 325, 458, 536, 700
XapI RAATTY 1 cut(s) 1155
XceI RCATGY 1 cut(s) 640
XcmI CCANNNNNNNNNTGG 1 cut(s) 1141
XspI CTAG 2 cut(s) 689, 831
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.