Rw1G041020

START domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0003829
Physical Location & Seq
Forward (+)
68055903 .. 68058094
2192 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G041020.1

Sequence Viewer

Length: 1308 bp
ATGGGCCCTCTGCATGAGATTTTGGAGATGGTGAAAATAGAGTACACGGACTTTGTGGTGTTTCTGGGTCCTCTGTGGACGGCTTTCCTTATTGGTATTGTGATTGGTTGGGCATGGAAGCCGAAGTGGGCAACATTTGGTCAGGGAAAACTAGGCTCCTCTGTTTCTAAAGTCTTGGATTCGTCTATGCCTACTTCGCCGGCTAGGTCTGTGATGTCACCACCCTTAAAAGGGTTCTCATCAGCACCGTGCTTGTTCCAAGGCTATGAAGCTTGGTTCATTAACCATGCAGTGCAGAAGAGGCTTTCTCCCTCGCCTATCGAATTTGATGACCACAGTACCTGTACATCGCAGATGAATGAGAAGAAGTCTAATGTTGTGGCTCAGGAGGATGTAGAAAATCTATGTCGGCTTGTAGATATGAAAGATGGAGGTCCGGCTTGGATACATATGATGGATCGTTCAACTCCAACTATGGCCTACCAAGCTTGGCGTAGGGATCCAAAGATTGGTCCTCCTCAATATCGTAACAGGACTGTGTATGAGGATGCAACACCGGAGCTAGTGAGGGACTTTTTCTGGGATGATGAGTTCCGGTTAAGGTGGGACAACATGCTTAGTGACGCCACAACCTTAGATGAGTGCCCAACCACAGGAACCATGGTTGTTCAGTGGATAAAGAAGTTCCCGTTCTTCTGTAAAGACAGGGAATACATAATAGGACGTCGAATTTGGGAGTCAGGAAGGAAGTATTATTGTGTGACAAAGGGAGTACCCTATCCTTCTATTCAAAGGCATGACAAACCAAGACGTGTGGATCACTACTACTCAAGTTGGTGCATTCAAGCAGTGGAATCAAGACACGGCAATGGCCAGCTGACTGCATGTGAAGTTCAACTGTTTCATCAAGAGGAAACGGGAATCCCATGGGAAATTGCAAAACTTGCTGTACGAAAAGGATTGTGGGATACTGTAAAGAACATAGAGTCTGGTTTACGTGCTTACCAGAAAGAGAGAGCGTTAGGTGGAATAATTTCTCGGCCTGCCTTTCTGGCTCAGATCAACACCAAAATAAATCTAGAGTACCTGAAAGCCTCCGCTGGCACTGAGGATTCGTCACAAACTGCAGTGGTGACTACATCTGACAAACCTTTCAGTGGGAACATACGGAATCTCATAATTGTTGGTGGGAGTATTCTCCTTGCATGCACTCTTGACCGGGGGTTTCTGGTGAAGACAGTAATATTCGGTGTGGCCGATGTGGCCAGGAAGTTTGCAAATGTGGGGAAGAAGTCAAAACCAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
START

Protein Analysis

435

Amino Acids

49.31

Weight (kDa)

8.97

Isoelectric Point (pI)

46.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
START PF01852 145 - 298 1.6e-06 START domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 727
AccB7I CCANNNNNTGG 1 cut(s) 509
AciI CCGC 1 cut(s) 1098
AclWI GGATC 4 cut(s) 465, 494, 507, 825
AcoI YGGCCR 3 cut(s) 871, 1254, 1263
AcsI RAATTY 2 cut(s) 323, 729
AcyI GRCGYC 2 cut(s) 624, 724
AfaI GTAC 6 cut(s) 44, 340, 346, 774, 951, 1085
AfiI CCNNNNNNNGG 5 cut(s) 230, 231, 509, 653, 1157
AflIII ACRYGT 1 cut(s) 811
AgsI TTSAA 4 cut(s) 465, 791, 845, 896
AjiI CACGTC 1 cut(s) 812
AjnI CCWGG 1 cut(s) 1265
AloI GAACNNNNNNTCC 2 cut(s) 575, 607
AluBI AGCT 4 cut(s) 272, 488, 562, 877
AluI AGCT 4 cut(s) 272, 488, 562, 877
AlwI GGATC 4 cut(s) 465, 494, 507, 825
AlwNI CAGNNNCTG 1 cut(s) 342
AoxI GGCC 6 cut(s) 4, 477, 871, 1040, 1254, 1263
ApaI GGGCCC 1 cut(s) 8
ApoI RAATTY 2 cut(s) 323, 729
ArsI GACNNNNNNTTYG 2 cut(s) 714, 746
Asp700I GAANNNNTTC 1 cut(s) 1033
AspS9I GGNCC 5 cut(s) 4, 5, 68, 434, 512
AsuC2I CCSGG 1 cut(s) 1220
AsuHPI GGTGA 4 cut(s) 43, 210, 1144, 1243
AvaII GGWCC 3 cut(s) 68, 434, 512
BaeGI GKGCMC 2 cut(s) 8, 647
BaeI ACNNNNGTAYC 2 cut(s) 322, 355
BalI TGGCCA 2 cut(s) 873, 1265
BamHI GGATCC 1 cut(s) 499
BanII GRGCYC 1 cut(s) 8
BbsI GAAGAC 1 cut(s) 1241
BccI CCATC 3 cut(s) 22, 422, 448
BceAI ACGGC 2 cut(s) 96, 880
BciT130I CCWGG 1 cut(s) 1267
BciVI GTATCC 2 cut(s) 438, 961
BcnI CCSGG 1 cut(s) 1220
BfaI CTAG 4 cut(s) 152, 204, 563, 1079
BfmI CTRYAG 1 cut(s) 1125
BfuI GTATCC 2 cut(s) 438, 961
BglI GCCNNNNNGGC 2 cut(s) 1052, 1262
Bme1390I CCNGG 2 cut(s) 1220, 1267
Bme18I GGWCC 3 cut(s) 68, 434, 512
BmgBI CACGTC 1 cut(s) 812
BmgT120I GGNCC 5 cut(s) 4, 5, 68, 434, 512
BmiI GGNNCC 5 cut(s) 6, 69, 157, 501, 658
BmrFI CCNGG 2 cut(s) 1220, 1267
BmsI GCATC 1 cut(s) 538
BpiI GAAGAC 1 cut(s) 1241
BplI GAGNNNNNCTC 2 cut(s) 292, 324
Bpu10I CCTNAGC 1 cut(s) 384
BpuEI CTTGAG 1 cut(s) 814
BpuMI CCSGG 1 cut(s) 1220
BsaAI YACGTR 1 cut(s) 998
BsaHI GRCGYC 2 cut(s) 624, 724
BsaJI CCNNGG 4 cut(s) 259, 660, 926, 1219
BsaWI WCCGGW 2 cut(s) 556, 594
Bsc4I CCNNNNNNNGG 5 cut(s) 230, 231, 509, 653, 1157
Bse118I RCCGGY 1 cut(s) 199
Bse3DI GCAATG 1 cut(s) 874
BseBI CCWGG 1 cut(s) 1267
BseDI CCNNGG 4 cut(s) 259, 660, 926, 1219
BseGI GGATG 3 cut(s) 397, 553, 589
BseLI CCNNNNNNNGG 5 cut(s) 230, 231, 509, 653, 1157
BseMI GCAATG 1 cut(s) 874
BseMII CTCAG 3 cut(s) 398, 1070, 1098
BseRI GAGGAG 2 cut(s) 148, 507
BseSI GKGCMC 2 cut(s) 8, 647
BsgI GTGCAG 1 cut(s) 314
BshFI GGCC 6 cut(s) 6, 479, 873, 1042, 1256, 1265
BsiSI CCGG 5 cut(s) 200, 437, 557, 595, 1219
BslFI GGGAC 2 cut(s) 584, 620
BslI CCNNNNNNNGG 5 cut(s) 230, 231, 509, 653, 1157
BsmFI GGGAC 2 cut(s) 584, 620
BsmI GAATGC 1 cut(s) 840
BsnI GGCC 6 cut(s) 6, 479, 873, 1042, 1256, 1265
Bsp120I GGGCCC 1 cut(s) 4
Bsp1286I GDGCHC 2 cut(s) 8, 647
Bsp1407I TGTACA 1 cut(s) 344
Bsp143I GATC 4 cut(s) 457, 499, 817, 1059
Bsp19I CCATGG 2 cut(s) 660, 926
BspACI CCGC 1 cut(s) 1098
BspANI GGCC 6 cut(s) 6, 479, 873, 1042, 1256, 1265
BspCNI CTCAG 3 cut(s) 397, 1069, 1099
BspLI GGNNCC 5 cut(s) 6, 69, 157, 501, 658
BspMAI CTGCAG 1 cut(s) 1129
BspPI GGATC 4 cut(s) 465, 494, 507, 825
BsrDI GCAATG 1 cut(s) 874
BsrFI RCCGGY 1 cut(s) 199
BsrGI TGTACA 1 cut(s) 344
BssAI RCCGGY 1 cut(s) 199
BssECI CCNNGG 4 cut(s) 259, 660, 926, 1219
BssMI GATC 4 cut(s) 457, 499, 817, 1059
BssNI GRCGYC 2 cut(s) 624, 724
BssT1I CCWWGG 3 cut(s) 259, 660, 926
Bst2UI CCWGG 1 cut(s) 1267
Bst4CI ACNGT 6 cut(s) 249, 338, 538, 900, 973, 1240
Bst6I CTCTTC 1 cut(s) 293
BstACI GRCGYC 2 cut(s) 624, 724
BstAPI GCANNNNNTGC 1 cut(s) 944
BstAUI TGTACA 1 cut(s) 344
BstBAI YACGTR 1 cut(s) 998
BstC8I GCNNGC 5 cut(s) 201, 875, 1044, 1102, 1207
BstDEI CTNAG 5 cut(s) 384, 617, 634, 1056, 1107
BstDSI CCRYGG 2 cut(s) 660, 926
BstF5I GGATG 3 cut(s) 397, 553, 589
BstKTI GATC 4 cut(s) 460, 502, 820, 1062
BstMBI GATC 4 cut(s) 457, 499, 817, 1059
BstMWI GCNNNNNNNGC 6 cut(s) 196, 301, 485, 944, 1052, 1262
BstNI CCWGG 1 cut(s) 1267
BstNSI RCATGY 3 cut(s) 616, 888, 1209
BstSCI CCNGG 2 cut(s) 1218, 1265
BstSFI CTRYAG 1 cut(s) 1125
BstSLI GKGCMC 2 cut(s) 8, 647
BstV2I GAAGAC 1 cut(s) 1241
BstX2I RGATCY 1 cut(s) 499
BstYI RGATCY 1 cut(s) 499
BsuI GTATCC 2 cut(s) 438, 961
BsuRI GGCC 6 cut(s) 6, 479, 873, 1042, 1256, 1265
BtgI CCRYGG 2 cut(s) 660, 926
BtgZI GCGATG 1 cut(s) 333
BtrI CACGTC 1 cut(s) 812
BtsCI GGATG 3 cut(s) 397, 553, 589
BtsI GCAGTG 3 cut(s) 297, 855, 1134
BtsIMutI CAGTG 6 cut(s) 297, 677, 855, 1104, 1134, 1162
Cac8I GCNNGC 5 cut(s) 201, 875, 1044, 1102, 1207
CaiI CAGNNNCTG 1 cut(s) 342
Cfr10I RCCGGY 1 cut(s) 199
Cfr13I GGNCC 5 cut(s) 4, 5, 68, 434, 512
CseI GACGC 1 cut(s) 632
Csp6I GTAC 6 cut(s) 43, 339, 345, 773, 950, 1084
CspCI CAANNNNNGTGG 2 cut(s) 795, 830
CviAII CATG 9 cut(s) 14, 114, 287, 613, 661, 797, 885, 927, 1206
CviQI GTAC 6 cut(s) 43, 339, 345, 773, 950, 1084
DdeI CTNAG 5 cut(s) 384, 617, 634, 1056, 1107
DpnI GATC 4 cut(s) 459, 501, 819, 1061
DpnII GATC 4 cut(s) 457, 499, 817, 1059
EaeI YGGCCR 3 cut(s) 871, 1254, 1263
Eam1104I CTCTTC 1 cut(s) 293
EarI CTCTTC 1 cut(s) 293
Eco130I CCWWGG 3 cut(s) 259, 660, 926
Eco24I GRGCYC 1 cut(s) 8
Eco47I GGWCC 3 cut(s) 68, 434, 512
EcoO109I RGGNCCY 2 cut(s) 5, 68
EcoRII CCWGG 1 cut(s) 1265
EcoT14I CCWWGG 3 cut(s) 259, 660, 926
EcoT38I GRGCYC 1 cut(s) 8
ErhI CCWWGG 3 cut(s) 259, 660, 926
FaeI CATG 9 cut(s) 17, 117, 290, 616, 664, 800, 888, 930, 1209
FaqI GGGAC 2 cut(s) 584, 620
FatI CATG 9 cut(s) 13, 113, 286, 612, 660, 796, 884, 926, 1205
FauNDI CATATG 1 cut(s) 450
FokI GGATG 3 cut(s) 404, 560, 596
FriOI GRGCYC 1 cut(s) 8
FspBI CTAG 4 cut(s) 152, 204, 563, 1079
HaeIII GGCC 6 cut(s) 6, 479, 873, 1042, 1256, 1265
HapII CCGG 5 cut(s) 200, 437, 557, 595, 1219
HgaI GACGC 1 cut(s) 632
Hin1I GRCGYC 2 cut(s) 624, 724
Hin1II CATG 9 cut(s) 17, 117, 290, 616, 664, 800, 888, 930, 1209
HindIII AAGCTT 2 cut(s) 270, 486
HinfI GANTC 7 cut(s) 179, 737, 854, 921, 986, 1112, 1171
HpaII CCGG 5 cut(s) 200, 437, 557, 595, 1219
HphI GGTGA 4 cut(s) 43, 210, 1144, 1243
Hpy166II GTNNAC 3 cut(s) 45, 78, 995
Hpy188I TCNGA 2 cut(s) 1059, 1144
Hpy188III TCNNGA 6 cut(s) 386, 741, 858, 908, 1079, 1214
Hpy8I GTNNAC 3 cut(s) 45, 78, 995
Hpy99I CGWCG 1 cut(s) 729
HpyAV CCTTC 2 cut(s) 738, 792
HpyCH4III ACNGT 6 cut(s) 249, 338, 538, 900, 973, 1240
HpyCH4IV ACGT 3 cut(s) 724, 811, 997
HpyF10VI GCNNNNNNNGC 6 cut(s) 196, 301, 485, 944, 1052, 1262
HpyF3I CTNAG 5 cut(s) 384, 617, 634, 1056, 1107
HpySE526I ACGT 3 cut(s) 724, 811, 997
Hsp92I GRCGYC 2 cut(s) 624, 724
Hsp92II CATG 9 cut(s) 17, 117, 290, 616, 664, 800, 888, 930, 1209
KroI GCCGGC 1 cut(s) 199
KroNI GCCGGC 1 cut(s) 201
Kzo9I GATC 4 cut(s) 457, 499, 817, 1059
LmnI GCTCC 2 cut(s) 161, 559
LweI GCATC 1 cut(s) 538
MaeI CTAG 4 cut(s) 152, 204, 563, 1079
MaeII ACGT 3 cut(s) 724, 811, 997
MaeIII GTNAC 6 cut(s) 216, 527, 620, 760, 1116, 1132
MalI GATC 4 cut(s) 459, 501, 819, 1061
MboI GATC 4 cut(s) 457, 499, 817, 1059
MboII GAAGA 5 cut(s) 310, 376, 685, 1246, 1300
MflI RGATCY 1 cut(s) 499
MhlI GDGCHC 2 cut(s) 8, 647
MlsI TGGCCA 2 cut(s) 873, 1265
MluCI AATT 5 cut(s) 323, 729, 933, 1032, 1179
MluNI TGGCCA 2 cut(s) 873, 1265
MlyI GAGTC 2 cut(s) 746, 995
MmeI TCCRAC 1 cut(s) 494
Mox20I TGGCCA 2 cut(s) 873, 1265
MroNI GCCGGC 1 cut(s) 199
MroXI GAANNNNTTC 1 cut(s) 1033
MscI TGGCCA 2 cut(s) 873, 1265
MseI TTAA 3 cut(s) 227, 282, 599
MslI CAYNNNNRTG 1 cut(s) 867
Msp20I TGGCCA 2 cut(s) 873, 1265
MspA1I CMGCKG 2 cut(s) 877, 1100
MspI CCGG 5 cut(s) 200, 437, 557, 595, 1219
MspR9I CCNGG 2 cut(s) 1220, 1267
Mva1269I GAATGC 1 cut(s) 840
MvaI CCWGG 1 cut(s) 1267
MwoI GCNNNNNNNGC 6 cut(s) 196, 301, 485, 944, 1052, 1262
NaeI GCCGGC 1 cut(s) 201
NciI CCSGG 1 cut(s) 1220
NcoI CCATGG 2 cut(s) 660, 926
NdeI CATATG 1 cut(s) 450
NdeII GATC 4 cut(s) 457, 499, 817, 1059
NgoMIV GCCGGC 1 cut(s) 199
NlaIII CATG 9 cut(s) 17, 117, 290, 616, 664, 800, 888, 930, 1209
NlaIV GGNNCC 5 cut(s) 6, 69, 157, 501, 658
NmeAIII GCCGAG 1 cut(s) 1018
NmuCI GTSAC 5 cut(s) 216, 620, 760, 1116, 1132
NspI RCATGY 3 cut(s) 616, 888, 1209
PaeI GCATGC 1 cut(s) 1209
PcsI WCGNNNNNNNCGW 1 cut(s) 1254
PctI GAATGC 1 cut(s) 840
PdiI GCCGGC 1 cut(s) 201
PdmI GAANNNNTTC 1 cut(s) 1033
PfeI GAWTC 5 cut(s) 179, 854, 921, 1112, 1171
PflMI CCANNNNNTGG 1 cut(s) 509
PleI GAGTC 2 cut(s) 745, 994
PpsI GAGTC 2 cut(s) 745, 994
Ppu21I YACGTR 1 cut(s) 998
PpuMI RGGWCCY 1 cut(s) 68
Psp5II RGGWCCY 1 cut(s) 68
Psp6I CCWGG 1 cut(s) 1265
PspGI CCWGG 1 cut(s) 1265
PspN4I GGNNCC 5 cut(s) 6, 69, 157, 501, 658
PspOMI GGGCCC 1 cut(s) 4
PspPI GGNCC 5 cut(s) 4, 5, 68, 434, 512
PspPPI RGGWCCY 1 cut(s) 68
PstI CTGCAG 1 cut(s) 1129
PstNI CAGNNNCTG 1 cut(s) 342
PsuI RGATCY 1 cut(s) 499
PvuII CAGCTG 1 cut(s) 877
RsaI GTAC 6 cut(s) 44, 340, 346, 774, 951, 1085
RsaNI GTAC 6 cut(s) 43, 339, 345, 773, 950, 1084
RseI CAYNNNNRTG 1 cut(s) 867
SaqAI TTAA 3 cut(s) 227, 282, 599
Sau3AI GATC 4 cut(s) 457, 499, 817, 1059
Sau96I GGNCC 5 cut(s) 4, 5, 68, 434, 512
SchI GAGTC 2 cut(s) 746, 995
ScrFI CCNGG 2 cut(s) 1220, 1267
SduI GDGCHC 2 cut(s) 8, 647
SfaNI GCATC 1 cut(s) 538
SfcI CTRYAG 1 cut(s) 1125
SfiI GGCCNNNNNGGCC 1 cut(s) 1262
SinI GGWCC 3 cut(s) 68, 434, 512
SmiMI CAYNNNNRTG 1 cut(s) 867
SmlI CTYRAG 1 cut(s) 829
SmoI CTYRAG 1 cut(s) 829
SphI GCATGC 1 cut(s) 1209
Sse9I AATT 5 cut(s) 323, 729, 933, 1032, 1179
SsiI CCGC 1 cut(s) 1098
SspI AATATT 1 cut(s) 1245
SspMI CTAG 4 cut(s) 152, 204, 563, 1079
StyD4I CCNGG 2 cut(s) 1218, 1265
StyI CCWWGG 3 cut(s) 259, 660, 926
TaaI ACNGT 6 cut(s) 249, 338, 538, 900, 973, 1240
TaiI ACGT 3 cut(s) 727, 814, 1000
TaqI TCGA 2 cut(s) 321, 727
TasI AATT 5 cut(s) 323, 729, 933, 1032, 1179
TatI WGTACW 2 cut(s) 42, 344
TfiI GAWTC 5 cut(s) 179, 854, 921, 1112, 1171
Tru1I TTAA 3 cut(s) 227, 282, 599
Tru9I TTAA 3 cut(s) 227, 282, 599
TscAI CASTG 6 cut(s) 297, 677, 855, 1111, 1134, 1162
TseFI GTSAC 5 cut(s) 216, 620, 760, 1116, 1132
Tsp45I GTSAC 5 cut(s) 216, 620, 760, 1116, 1132
TspDTI ATGAA 5 cut(s) 268, 282, 371, 437, 893
TspGWI ACGGA 2 cut(s) 62, 1183
TspRI CASTG 6 cut(s) 297, 677, 855, 1111, 1134, 1162
Van91I CCANNNNNTGG 1 cut(s) 509
VpaK11BI GGWCC 3 cut(s) 68, 434, 512
XapI RAATTY 2 cut(s) 323, 729
XbaI TCTAGA 1 cut(s) 1078
XceI RCATGY 3 cut(s) 616, 888, 1209
XcmI CCANNNNNNNNNTGG 1 cut(s) 658
XmnI GAANNNNTTC 1 cut(s) 1033
XspI CTAG 4 cut(s) 152, 204, 563, 1079
ZraI GACGTC 1 cut(s) 725
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.