Rmu_ssc0000110.1_g000016

START domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000110.1
OG0003829
Physical Location & Seq
Forward (+)
82670 .. 85457
2788 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000110.1_g000016.1.cds

Sequence Viewer

Length: 1284 bp
atggctttggcgtcggctttgctggagattctgcgaagacccacgattgttgaagtgctgggggagctcgtcaagttcataacccctctttggattgctgtgattgtcggggttttcgttgggtgggcgtggcgccccaaatgggtgaccgtggttggtagagaattgttggatagctccacacaaaaggagtcatcttcctcatctccgcccgcaccatccacgacgtgtgggatttcgatttcgagtttgagttcgttgctgccgaattgcattccttgggctgctgacgatagaaatctgaaccaggcacctaccaccaataatgattgcagctcctcacagattgaacaagaagaatcctgtaatgtctccaatgaggatctacgccacatatgtcagcttgtggaggagaaagatggcggtcctgcttggattcatatgatggatcggtcaaccccaactatgggatatcaagcttggcgtagggatcctcagactggacctccacaataccgaagtagaactgtattcgaagatgcttcacctgagacagtgagggatttcttttgggatgatgaatttcgtgcaaagtgggatgatatgcttattcatgcttctactttggaagaatgcccgactactggaaccatgctagtgcactgggtccgcaagtttccattcttttgtagcgacagagattacacgataggccgtcgcatttgggaatcggggcgatcatactactgtgtaactaagggagttccttcctctgctgtgccaaggcgtgacaaacctagacgtgttgatctgtactattcaagttggtgcatacgtgcagttgaatcaaaaagaggggatggccagcttacttcatgtgaggttttgctgtttcaccatgaagatatgggtattccatgggagattgcgaaacttggtgttcgtcaaggtatgtggggagctgttaaaaagattgaaccaggcctacgtttatatcaaaaggaaagagccgctgaagcaccactttcgcggtgtgctaaaatggctcagatcaacacgaaagtcagtgctgattacttgagatcggtagaaaacagcactaaggattccatggaggttgaaaattcagattcagcggagaaaccaggaaggaggaatattcctaaagctctagttgtcggaggggcaatagttcttgcttgcagtcttgatcggggcctcttgactaaggcagttatatttggagtagctcgaagatttgccaagattgggaggaggttgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
START

Protein Analysis

427

Amino Acids

48.02

Weight (kDa)

8.39

Isoelectric Point (pI)

55.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 132, 310
AccII CGCG 1 cut(s) 1030
AciI CCGC 7 cut(s) 209, 213, 423, 670, 1011, 1030, 1136
AclWI GGATC 4 cut(s) 390, 456, 485, 498
AcoI YGGCCR 1 cut(s) 862
AcsI RAATTY 2 cut(s) 581, 1123
AcuI CTGAAG 1 cut(s) 1035
AcyI GRCGYC 2 cut(s) 11, 133
AdeI CACNNNGTG 1 cut(s) 228
AfaI GTAC 1 cut(s) 815
AfiI CCNNNNNNNGG 7 cut(s) 90, 142, 466, 467, 500, 644, 1269
AflIII ACRYGT 2 cut(s) 227, 802
AgsI TTSAA 6 cut(s) 53, 350, 822, 845, 977, 1121
AjiI CACGTC 2 cut(s) 228, 803
AjnI CCWGG 3 cut(s) 306, 979, 1144
AluBI AGCT 9 cut(s) 67, 177, 336, 403, 479, 868, 962, 1169, 1250
AluI AGCT 9 cut(s) 67, 177, 336, 403, 479, 868, 962, 1169, 1250
Alw21I GWGCWC 2 cut(s) 69, 663
Alw26I GTCTC 2 cut(s) 376, 545
Alw44I GTGCAC 1 cut(s) 659
AlwI GGATC 4 cut(s) 390, 456, 485, 498
AoxI GGCC 4 cut(s) 712, 862, 982, 1216
ApaLI GTGCAC 1 cut(s) 659
ApeKI GCWGC 3 cut(s) 262, 284, 333
ApoI RAATTY 2 cut(s) 581, 1123
AspLEI GCGC 1 cut(s) 135
AspS9I GGNCC 4 cut(s) 425, 503, 667, 1216
AsuHPI GGTGA 3 cut(s) 157, 537, 887
AsuII TTCGAA 1 cut(s) 534
AvaII GGWCC 3 cut(s) 425, 503, 667
BaeGI GKGCMC 1 cut(s) 663
BalI TGGCCA 1 cut(s) 864
BamHI GGATCC 1 cut(s) 490
BanI GGYRCC 2 cut(s) 132, 310
BanII GRGCYC 1 cut(s) 69
BbsI GAAGAC 1 cut(s) 43
Bbv12I GWGCWC 2 cut(s) 69, 663
BbvI GCAGC 3 cut(s) 249, 271, 345
BccI CCATC 4 cut(s) 226, 413, 439, 854
BceAI ACGGC 1 cut(s) 699
BcgI CGANNNNNNTGC 2 cut(s) 1008, 1042
BciT130I CCWGG 3 cut(s) 308, 981, 1146
BcoDI GTCTC 2 cut(s) 376, 545
BfaI CTAG 3 cut(s) 656, 798, 1172
BfoI RGCGCY 1 cut(s) 136
BisI GCNGC 4 cut(s) 263, 285, 334, 1011
BlsI GCNGC 4 cut(s) 264, 286, 335, 1012
Bme1390I CCNGG 3 cut(s) 308, 981, 1146
Bme18I GGWCC 3 cut(s) 425, 503, 667
BmgBI CACGTC 2 cut(s) 228, 803
BmgT120I GGNCC 4 cut(s) 425, 503, 667, 1216
BmiI GGNNCC 6 cut(s) 134, 312, 492, 649, 668, 1217
BmrFI CCNGG 3 cut(s) 308, 981, 1146
BmrI ACTGGG 1 cut(s) 673
BmsI GCATC 1 cut(s) 529
BmuI ACTGGG 1 cut(s) 673
BpiI GAAGAC 1 cut(s) 43
BpmI CTGGAG 1 cut(s) 44
Bpu14I TTCGAA 1 cut(s) 534
BpuEI CTTGAG 1 cut(s) 1099
BsaAI YACGTR 1 cut(s) 836
BsaBI GATNNNNATC 1 cut(s) 297
BsaHI GRCGYC 2 cut(s) 11, 133
BsaJI CCNNGG 5 cut(s) 150, 278, 782, 917, 1110
BsaXI ACNNNNNCTCC 2 cut(s) 490, 520
Bsc4I CCNNNNNNNGG 7 cut(s) 90, 142, 466, 467, 500, 644, 1269
Bse1I ACTGG 3 cut(s) 505, 649, 668
Bse8I GATNNNNATC 1 cut(s) 297
BseBI CCWGG 3 cut(s) 308, 981, 1146
BseDI CCNNGG 5 cut(s) 150, 278, 782, 917, 1110
BseGI GGATG 4 cut(s) 218, 580, 604, 865
BseJI GATNNNNATC 1 cut(s) 297
BseLI CCNNNNNNNGG 7 cut(s) 90, 142, 466, 467, 500, 644, 1269
BseMII CTCAG 3 cut(s) 509, 540, 1061
BseNI ACTGG 3 cut(s) 505, 649, 668
BseRI GAGGAG 2 cut(s) 328, 425
BseSI GKGCMC 1 cut(s) 663
BseXI GCAGC 3 cut(s) 249, 271, 345
BseYI CCCAGC 1 cut(s) 58
BsgI GTGCAG 1 cut(s) 858
Bsh1236I CGCG 1 cut(s) 1030
BshFI GGCC 4 cut(s) 714, 864, 984, 1218
BshNI GGYRCC 2 cut(s) 132, 310
BsiHKAI GWGCWC 2 cut(s) 69, 663
BslI CCNNNNNNNGG 7 cut(s) 90, 142, 466, 467, 500, 644, 1269
BsmAI GTCTC 2 cut(s) 376, 545
BsmI GAATGC 2 cut(s) 273, 638
BsnI GGCC 4 cut(s) 714, 864, 984, 1218
Bsp119I TTCGAA 1 cut(s) 534
Bsp1286I GDGCHC 2 cut(s) 69, 663
Bsp143I GATC 8 cut(s) 382, 448, 490, 737, 808, 1050, 1082, 1210
Bsp19I CCATGG 2 cut(s) 917, 1110
BspACI CCGC 7 cut(s) 209, 213, 423, 670, 1011, 1030, 1136
BspANI GGCC 4 cut(s) 714, 864, 984, 1218
BspCNI CTCAG 3 cut(s) 508, 541, 1060
BspFNI CGCG 1 cut(s) 1030
BspLI GGNNCC 6 cut(s) 134, 312, 492, 649, 668, 1217
BspPI GGATC 4 cut(s) 390, 456, 485, 498
BspT104I TTCGAA 1 cut(s) 534
BspT107I GGYRCC 2 cut(s) 132, 310
BsrI ACTGG 3 cut(s) 505, 649, 668
BssECI CCNNGG 5 cut(s) 150, 278, 782, 917, 1110
BssMI GATC 8 cut(s) 382, 448, 490, 737, 808, 1050, 1082, 1210
BssNI GRCGYC 2 cut(s) 11, 133
BssT1I CCWWGG 4 cut(s) 278, 782, 917, 1110
Bst2UI CCWGG 3 cut(s) 308, 981, 1146
Bst4CI ACNGT 4 cut(s) 151, 529, 556, 749
BstACI GRCGYC 2 cut(s) 11, 133
BstBAI YACGTR 1 cut(s) 836
BstBI TTCGAA 1 cut(s) 534
BstC8I GCNNGC 3 cut(s) 213, 866, 1201
BstDEI CTNAG 6 cut(s) 495, 549, 756, 1047, 1101, 1227
BstDSI CCRYGG 3 cut(s) 150, 917, 1110
BstEII GGTNACC 1 cut(s) 145
BstF5I GGATG 4 cut(s) 218, 580, 604, 865
BstFNI CGCG 1 cut(s) 1030
BstH2I RGCGCY 1 cut(s) 136
BstHHI GCGC 1 cut(s) 135
BstKTI GATC 8 cut(s) 385, 451, 493, 740, 811, 1053, 1085, 1213
BstMAI GTCTC 2 cut(s) 376, 545
BstMBI GATC 8 cut(s) 382, 448, 490, 737, 808, 1050, 1082, 1210
BstMWI GCNNNNNNNGC 3 cut(s) 64, 1016, 1043
BstNI CCWGG 3 cut(s) 308, 981, 1146
BstPI GGTNACC 1 cut(s) 145
BstSCI CCNGG 3 cut(s) 306, 979, 1144
BstSLI GKGCMC 1 cut(s) 663
BstUI CGCG 1 cut(s) 1030
BstV1I GCAGC 3 cut(s) 249, 271, 345
BstV2I GAAGAC 1 cut(s) 43
BstX2I RGATCY 2 cut(s) 382, 490
BstYI RGATCY 2 cut(s) 382, 490
BsuRI GGCC 4 cut(s) 714, 864, 984, 1218
BtgI CCRYGG 3 cut(s) 150, 917, 1110
BtrI CACGTC 2 cut(s) 228, 803
BtsCI GGATG 4 cut(s) 218, 580, 604, 865
BtsIMutI CAGTG 3 cut(s) 561, 661, 1072
Cac8I GCNNGC 3 cut(s) 213, 866, 1201
CfoI GCGC 1 cut(s) 135
Cfr13I GGNCC 4 cut(s) 425, 503, 667, 1216
Csp6I GTAC 1 cut(s) 814
CspCI CAANNNNNGTGG 4 cut(s) 31, 66, 935, 970
CviAII CATG 6 cut(s) 614, 652, 876, 899, 918, 1111
CviQI GTAC 1 cut(s) 814
DdeI CTNAG 6 cut(s) 495, 549, 756, 1047, 1101, 1227
DinI GGCGCC 1 cut(s) 134
DpnI GATC 8 cut(s) 384, 450, 492, 739, 810, 1052, 1084, 1212
DpnII GATC 8 cut(s) 382, 448, 490, 737, 808, 1050, 1082, 1210
DraIII CACNNNGTG 1 cut(s) 228
EaeI YGGCCR 1 cut(s) 862
EciI GGCGGA 1 cut(s) 198
Ecl136II GAGCTC 1 cut(s) 67
Eco130I CCWWGG 4 cut(s) 278, 782, 917, 1110
Eco147I AGGCCT 1 cut(s) 984
Eco24I GRGCYC 1 cut(s) 69
Eco32I GATATC 1 cut(s) 473
Eco47I GGWCC 3 cut(s) 425, 503, 667
Eco53kI GAGCTC 1 cut(s) 67
Eco57I CTGAAG 1 cut(s) 1035
Eco91I GGTNACC 1 cut(s) 145
EcoICRI GAGCTC 1 cut(s) 67
EcoO109I RGGNCCY 1 cut(s) 1216
EcoO65I GGTNACC 1 cut(s) 145
EcoRII CCWGG 3 cut(s) 306, 979, 1144
EcoRV GATATC 1 cut(s) 473
EcoT14I CCWWGG 4 cut(s) 278, 782, 917, 1110
EcoT38I GRGCYC 1 cut(s) 69
EgeI GGCGCC 1 cut(s) 134
EheI GGCGCC 1 cut(s) 134
ErhI CCWWGG 4 cut(s) 278, 782, 917, 1110
FaeI CATG 6 cut(s) 617, 655, 879, 902, 921, 1114
FalI AAGNNNNNCTT 2 cut(s) 1008, 1040
FatI CATG 6 cut(s) 613, 651, 875, 898, 917, 1110
FauI CCCGC 1 cut(s) 220
FauNDI CATATG 2 cut(s) 395, 441
Fnu4HI GCNGC 4 cut(s) 263, 285, 334, 1011
FokI GGATG 4 cut(s) 205, 587, 611, 872
FriOI GRGCYC 1 cut(s) 69
Fsp4HI GCNGC 4 cut(s) 263, 285, 334, 1011
FspBI CTAG 3 cut(s) 656, 798, 1172
GlaI GCGC 1 cut(s) 134
GluI GCNGC 4 cut(s) 263, 285, 334, 1011
GsaI CCCAGC 1 cut(s) 62
GsuI CTGGAG 1 cut(s) 44
HaeII RGCGCY 1 cut(s) 136
HaeIII GGCC 4 cut(s) 714, 864, 984, 1218
HhaI GCGC 1 cut(s) 135
Hin1I GRCGYC 2 cut(s) 11, 133
Hin1II CATG 6 cut(s) 617, 655, 879, 902, 921, 1114
Hin6I GCGC 1 cut(s) 133
HinP1I GCGC 1 cut(s) 133
HincII GTYRAC 1 cut(s) 456
HindII GTYRAC 1 cut(s) 456
HindIII AAGCTT 1 cut(s) 477
HinfI GANTC 8 cut(s) 28, 191, 359, 436, 728, 845, 1106, 1130
HphI GGTGA 3 cut(s) 157, 537, 887
Hpy166II GTNNAC 2 cut(s) 456, 661
Hpy188I TCNGA 5 cut(s) 303, 498, 1050, 1129, 1181
Hpy188III TCNNGA 2 cut(s) 1208, 1222
Hpy8I GTNNAC 2 cut(s) 456, 661
Hpy99I CGWCG 3 cut(s) 16, 229, 720
HpyAV CCTTC 2 cut(s) 777, 1143
HpyCH4III ACNGT 4 cut(s) 151, 529, 556, 749
HpyCH4IV ACGT 4 cut(s) 227, 802, 835, 988
HpyCH4V TGCA 7 cut(s) 273, 333, 590, 661, 831, 839, 1203
HpyF10VI GCNNNNNNNGC 3 cut(s) 64, 1016, 1043
HpyF3I CTNAG 6 cut(s) 495, 549, 756, 1047, 1101, 1227
HpySE526I ACGT 4 cut(s) 227, 802, 835, 988
Hsp92I GRCGYC 2 cut(s) 11, 133
Hsp92II CATG 6 cut(s) 617, 655, 879, 902, 921, 1114
HspAI GCGC 1 cut(s) 133
KasI GGCGCC 1 cut(s) 132
Kzo9I GATC 8 cut(s) 382, 448, 490, 737, 808, 1050, 1082, 1210
LmnI GCTCC 4 cut(s) 64, 182, 341, 959
Lsp1109I GCAGC 3 cut(s) 249, 271, 345
LweI GCATC 1 cut(s) 529
MaeI CTAG 3 cut(s) 656, 798, 1172
MaeII ACGT 4 cut(s) 227, 802, 835, 988
MaeIII GTNAC 3 cut(s) 145, 751, 788
MalI GATC 8 cut(s) 384, 450, 492, 739, 810, 1052, 1084, 1212
MboI GATC 8 cut(s) 382, 448, 490, 737, 808, 1050, 1082, 1210
MboII GAAGA 7 cut(s) 48, 189, 368, 548, 641, 914, 1266
MflI RGATCY 2 cut(s) 382, 490
MhlI GDGCHC 2 cut(s) 69, 663
MlsI TGGCCA 1 cut(s) 864
MluCI AATT 4 cut(s) 164, 268, 581, 1123
MluNI TGGCCA 1 cut(s) 864
Mly113I GGCGCC 1 cut(s) 133
MlyI GAGTC 1 cut(s) 200
MmeI TCCRAC 2 cut(s) 150, 1159
Mox20I TGGCCA 1 cut(s) 864
MscI TGGCCA 1 cut(s) 864
MseI TTAA 1 cut(s) 966
MslI CAYNNNNRTG 1 cut(s) 656
Msp20I TGGCCA 1 cut(s) 864
MspA1I CMGCKG 2 cut(s) 1013, 1136
MspR9I CCNGG 3 cut(s) 308, 981, 1146
Mva1269I GAATGC 2 cut(s) 273, 638
MvaI CCWGG 3 cut(s) 308, 981, 1146
MvnI CGCG 1 cut(s) 1030
MwoI GCNNNNNNNGC 3 cut(s) 64, 1016, 1043
NarI GGCGCC 1 cut(s) 133
NcoI CCATGG 2 cut(s) 917, 1110
NdeI CATATG 2 cut(s) 395, 441
NdeII GATC 8 cut(s) 382, 448, 490, 737, 808, 1050, 1082, 1210
NlaIII CATG 6 cut(s) 617, 655, 879, 902, 921, 1114
NlaIV GGNNCC 6 cut(s) 134, 312, 492, 649, 668, 1217
NmuCI GTSAC 2 cut(s) 145, 788
NspV TTCGAA 1 cut(s) 534
PceI AGGCCT 1 cut(s) 984
PcsI WCGNNNNNNNCGW 2 cut(s) 114, 263
PctI GAATGC 2 cut(s) 273, 638
PfeI GAWTC 7 cut(s) 28, 359, 436, 728, 845, 1106, 1130
PkrI GCNGC 4 cut(s) 264, 286, 335, 1012
PleI GAGTC 1 cut(s) 199
PluTI GGCGCC 1 cut(s) 136
PpsI GAGTC 1 cut(s) 199
Ppu21I YACGTR 1 cut(s) 836
Psp124BI GAGCTC 1 cut(s) 69
Psp6I CCWGG 3 cut(s) 306, 979, 1144
PspEI GGTNACC 1 cut(s) 145
PspFI CCCAGC 1 cut(s) 58
PspGI CCWGG 3 cut(s) 306, 979, 1144
PspN4I GGNNCC 6 cut(s) 134, 312, 492, 649, 668, 1217
PspPI GGNCC 4 cut(s) 425, 503, 667, 1216
PsrI GAACNNNNNNTAC 2 cut(s) 969, 1001
PsuI RGATCY 2 cut(s) 382, 490
RsaI GTAC 1 cut(s) 815
RsaNI GTAC 1 cut(s) 814
RseI CAYNNNNRTG 1 cut(s) 656
SacI GAGCTC 1 cut(s) 69
SaqAI TTAA 1 cut(s) 966
SatI GCNGC 4 cut(s) 263, 285, 334, 1011
Sau3AI GATC 8 cut(s) 382, 448, 490, 737, 808, 1050, 1082, 1210
Sau96I GGNCC 4 cut(s) 425, 503, 667, 1216
SchI GAGTC 1 cut(s) 200
ScrFI CCNGG 3 cut(s) 308, 981, 1146
SduI GDGCHC 2 cut(s) 69, 663
SfaNI GCATC 1 cut(s) 529
SfoI GGCGCC 1 cut(s) 134
SfuI TTCGAA 1 cut(s) 534
SinI GGWCC 3 cut(s) 425, 503, 667
SmiMI CAYNNNNRTG 1 cut(s) 656
SmlI CTYRAG 1 cut(s) 1078
SmoI CTYRAG 1 cut(s) 1078
Sse9I AATT 4 cut(s) 164, 268, 581, 1123
SseBI AGGCCT 1 cut(s) 984
SsiI CCGC 7 cut(s) 209, 213, 423, 670, 1011, 1030, 1136
SspDI GGCGCC 1 cut(s) 132
SspI AATATT 1 cut(s) 1159
SspMI CTAG 3 cut(s) 656, 798, 1172
SstI GAGCTC 1 cut(s) 69
StuI AGGCCT 1 cut(s) 984
StyD4I CCNGG 3 cut(s) 306, 979, 1144
StyI CCWWGG 4 cut(s) 278, 782, 917, 1110
TaaI ACNGT 4 cut(s) 151, 529, 556, 749
TaiI ACGT 4 cut(s) 230, 805, 838, 991
TaqI TCGA 4 cut(s) 239, 245, 534, 1252
TaqII GACCGA 1 cut(s) 441
TasI AATT 4 cut(s) 164, 268, 581, 1123
TatI WGTACW 1 cut(s) 813
TauI GCSGC 1 cut(s) 1013
TfiI GAWTC 7 cut(s) 28, 359, 436, 728, 845, 1106, 1130
Tru1I TTAA 1 cut(s) 966
Tru9I TTAA 1 cut(s) 966
TscAI CASTG 3 cut(s) 561, 668, 1072
TseFI GTSAC 2 cut(s) 145, 788
TseI GCWGC 3 cut(s) 262, 284, 333
Tsp45I GTSAC 2 cut(s) 145, 788
TspDTI ATGAA 6 cut(s) 67, 428, 594, 602, 864, 915
TspRI CASTG 3 cut(s) 561, 668, 1072
VneI GTGCAC 1 cut(s) 659
VpaK11BI GGWCC 3 cut(s) 425, 503, 667
XapI RAATTY 2 cut(s) 581, 1123
XspI CTAG 3 cut(s) 656, 798, 1172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.