Rh1AG461600

START domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0003829
Physical Location & Seq
Reverse (-)
67837752 .. 67840482
2731 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG461600.1

Sequence Viewer

Length: 1332 bp
ATGGCGGTAACCAACATGGATGCTCCGCATGAGATTTTGGAGATGGTGAAAATAGAGTACACGGACTTTGTGGTGTTTCTGGGTCCTCTGTGGACGGCTTTCCTTATTGGTATTGTGATTGGTTGGGCATGGAAGCCGAAGTGGGCAACATTTGGTCAGGAAAAACTAGGCTCCTCTGTTTCTAAAGTCTTGGATTCGTCTATGCCTACTTCGCCGGCTAGGTCTGTGATGTCACCACCTTTAAAAGGGTTCTCATCAGCACCGTGCTTGTTCCAAGGCTATGAAGCTTGGTTCATTAACCATGCAGTGCAGAAGAGGCTTTCTCCCTCGCCTATCGAATTTGATGACCACAGTACCTGTACATCGCAGATGAATGAGATGAATGAGAAGAAGTCTAATGTTGTGGCTCAGGAGGATGTAGAAAATCTATGTCGGCTTGTAGATATGAAAGATGGAGGTCCGACTTGGATACATATGATGGATCGTTCTACTCCAACTATGACCTACCAAGCTTGGCGTAGGGATCCGAAGATTGGTCCTCCTCAATATCGTAGCAGGACTGTGTATGAGGATGCAACGCCGGAGCTAGCGAGGGACTTTTTCTGGGATGATGAGTTCCGGTTAAGGTGGGACAACATGCTTAGTGATGCCACAACCTTAGATGAGTGCCCAACCACAGGAACCATGGTTGTTCAGTGGATAAAGAAGTTCCCGTTCTTCTGTAAAGACAGGGAATACATAATAGGACGTCGAATTTGGGAGTCAGGAAGGAAGTATTATTGTGTGACAAAGGGAGTACCCTATCCTTCTATTCAAAGGCATGACAAACCAAGACGTGTGGATCACTACTACTCAAGTTGGTGCATTCAAGCAGTGGAATCAAGACACGGCAATGGCCAGCTGACTGCATGTGAAGTTCAACTGTTTCATCAAGAGGAAACGGGAATCCCATGGGAAATTGCAAAACTTGCTGTACGAAAAGGAATGTGGGATACTGTAAAGAACATAGAGTCTGGTTTACGTGCTTACCAGAAAGAGAGAGCGTTAGGTGGAATAATTTCTCGGCCTGCCTTTCTGGCTCAGATCAACACCAAAATAAATCTAGAGTACCTGAAAGCCTCCGCTGGCACTGAGGATTCATCACAAACTGCAGTGGTGACTACATCTGACAAACCTTTCAGTGGGAACATACGGAATCTTATAATTGTTGGTGGGAGTATTCTCCTTGCATGCACTCTTGACCGGGGGTTTCTGGTGAAGACAGTAATATTCGGTGTGGCCGATGTGGCCAGGAAGTTTGCAAATGTGGGGAAGAAGTCAAAACCAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
START

Protein Analysis

443

Amino Acids

50.31

Weight (kDa)

8.7

Isoelectric Point (pI)

46.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
START PF01852 152 - 307 1.1e-06 START domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1202
AatII GACGTC 1 cut(s) 751
AciI CCGC 3 cut(s) 5, 26, 1122
AclWI GGATC 4 cut(s) 489, 518, 531, 849
AcoI YGGCCR 3 cut(s) 895, 1278, 1287
AcsI RAATTY 2 cut(s) 338, 753
AcyI GRCGYC 1 cut(s) 748
AfaI GTAC 6 cut(s) 59, 355, 361, 798, 975, 1109
AfiI CCNNNNNNNGG 4 cut(s) 245, 533, 677, 1181
AflIII ACRYGT 1 cut(s) 835
AgsI TTSAA 3 cut(s) 815, 869, 920
AjiI CACGTC 1 cut(s) 836
AjnI CCWGG 1 cut(s) 1289
AloI GAACNNNNNNTCC 2 cut(s) 599, 631
AluBI AGCT 4 cut(s) 287, 512, 586, 901
AluI AGCT 4 cut(s) 287, 512, 586, 901
AlwI GGATC 4 cut(s) 489, 518, 531, 849
AlwNI CAGNNNCTG 1 cut(s) 357
AoxI GGCC 4 cut(s) 895, 1064, 1278, 1287
ApoI RAATTY 2 cut(s) 338, 753
ArsI GACNNNNNNTTYG 2 cut(s) 738, 770
Asp700I GAANNNNTTC 1 cut(s) 1057
AspS9I GGNCC 3 cut(s) 83, 458, 536
AsuC2I CCSGG 1 cut(s) 1244
AsuHPI GGTGA 4 cut(s) 58, 225, 1168, 1267
AsuNHI GCTAGC 1 cut(s) 586
AvaII GGWCC 3 cut(s) 83, 458, 536
BaeGI GKGCMC 1 cut(s) 671
BaeI ACNNNNGTAYC 2 cut(s) 337, 370
BalI TGGCCA 2 cut(s) 897, 1289
BamHI GGATCC 1 cut(s) 523
BbsI GAAGAC 1 cut(s) 1265
BccI CCATC 3 cut(s) 37, 446, 472
BceAI ACGGC 2 cut(s) 111, 904
BciT130I CCWGG 1 cut(s) 1291
BciVI GTATCC 2 cut(s) 462, 985
BcnI CCSGG 1 cut(s) 1244
BfaI CTAG 4 cut(s) 167, 219, 587, 1103
BfmI CTRYAG 1 cut(s) 1149
BfuI GTATCC 2 cut(s) 462, 985
BglI GCCNNNNNGGC 2 cut(s) 1076, 1286
Bme1390I CCNGG 2 cut(s) 1244, 1291
Bme18I GGWCC 3 cut(s) 83, 458, 536
BmgBI CACGTC 1 cut(s) 836
BmgT120I GGNCC 3 cut(s) 83, 458, 536
BmiI GGNNCC 4 cut(s) 84, 172, 525, 682
BmrFI CCNGG 2 cut(s) 1244, 1291
BmsI GCATC 3 cut(s) 10, 562, 637
BmtI GCTAGC 1 cut(s) 590
BpiI GAAGAC 1 cut(s) 1265
BplI GAGNNNNNCTC 2 cut(s) 307, 339
Bpu10I CCTNAGC 1 cut(s) 408
BpuEI CTTGAG 1 cut(s) 838
BpuMI CCSGG 1 cut(s) 1244
BsaAI YACGTR 1 cut(s) 1022
BsaHI GRCGYC 1 cut(s) 748
BsaJI CCNNGG 4 cut(s) 274, 684, 950, 1243
BsaWI WCCGGW 1 cut(s) 618
Bsc4I CCNNNNNNNGG 4 cut(s) 245, 533, 677, 1181
Bse118I RCCGGY 1 cut(s) 214
Bse3DI GCAATG 1 cut(s) 898
BseBI CCWGG 1 cut(s) 1291
BseDI CCNNGG 4 cut(s) 274, 684, 950, 1243
BseGI GGATG 4 cut(s) 25, 421, 577, 613
BseLI CCNNNNNNNGG 4 cut(s) 245, 533, 677, 1181
BseMI GCAATG 1 cut(s) 898
BseMII CTCAG 3 cut(s) 422, 1094, 1122
BseRI GAGGAG 2 cut(s) 163, 531
BseSI GKGCMC 1 cut(s) 671
BsgI GTGCAG 1 cut(s) 329
BshFI GGCC 4 cut(s) 897, 1066, 1280, 1289
BsiSI CCGG 4 cut(s) 215, 581, 619, 1243
BslFI GGGAC 2 cut(s) 608, 644
BslI CCNNNNNNNGG 4 cut(s) 245, 533, 677, 1181
BsmFI GGGAC 2 cut(s) 608, 644
BsmI GAATGC 1 cut(s) 864
BsnI GGCC 4 cut(s) 897, 1066, 1280, 1289
Bsp1286I GDGCHC 1 cut(s) 671
Bsp1407I TGTACA 1 cut(s) 359
Bsp143I GATC 4 cut(s) 481, 523, 841, 1083
Bsp19I CCATGG 2 cut(s) 684, 950
BspACI CCGC 3 cut(s) 5, 26, 1122
BspANI GGCC 4 cut(s) 897, 1066, 1280, 1289
BspCNI CTCAG 3 cut(s) 421, 1093, 1123
BspLI GGNNCC 4 cut(s) 84, 172, 525, 682
BspMAI CTGCAG 1 cut(s) 1153
BspOI GCTAGC 1 cut(s) 590
BspPI GGATC 4 cut(s) 489, 518, 531, 849
BsrDI GCAATG 1 cut(s) 898
BsrFI RCCGGY 1 cut(s) 214
BsrGI TGTACA 1 cut(s) 359
BssAI RCCGGY 1 cut(s) 214
BssECI CCNNGG 4 cut(s) 274, 684, 950, 1243
BssMI GATC 4 cut(s) 481, 523, 841, 1083
BssNI GRCGYC 1 cut(s) 748
BssT1I CCWWGG 3 cut(s) 274, 684, 950
Bst2UI CCWGG 1 cut(s) 1291
Bst4CI ACNGT 6 cut(s) 264, 353, 562, 924, 997, 1264
Bst6I CTCTTC 1 cut(s) 308
BstACI GRCGYC 1 cut(s) 748
BstAPI GCANNNNNTGC 1 cut(s) 968
BstAUI TGTACA 1 cut(s) 359
BstBAI YACGTR 1 cut(s) 1022
BstC8I GCNNGC 6 cut(s) 216, 588, 899, 1068, 1126, 1231
BstDEI CTNAG 5 cut(s) 408, 641, 658, 1080, 1131
BstDSI CCRYGG 2 cut(s) 684, 950
BstEII GGTNACC 1 cut(s) 7
BstENI CCTNNNNNAGG 1 cut(s) 243
BstF5I GGATG 4 cut(s) 25, 421, 577, 613
BstKTI GATC 4 cut(s) 484, 526, 844, 1086
BstMBI GATC 4 cut(s) 481, 523, 841, 1083
BstMWI GCNNNNNNNGC 5 cut(s) 211, 316, 968, 1076, 1286
BstNI CCWGG 1 cut(s) 1291
BstNSI RCATGY 3 cut(s) 640, 912, 1233
BstPI GGTNACC 1 cut(s) 7
BstSCI CCNGG 2 cut(s) 1242, 1289
BstSFI CTRYAG 1 cut(s) 1149
BstSLI GKGCMC 1 cut(s) 671
BstV2I GAAGAC 1 cut(s) 1265
BstX2I RGATCY 1 cut(s) 523
BstYI RGATCY 1 cut(s) 523
BsuI GTATCC 2 cut(s) 462, 985
BsuRI GGCC 4 cut(s) 897, 1066, 1280, 1289
BtgI CCRYGG 2 cut(s) 684, 950
BtgZI GCGATG 1 cut(s) 348
BtrI CACGTC 1 cut(s) 836
BtsCI GGATG 4 cut(s) 25, 421, 577, 613
BtsI GCAGTG 3 cut(s) 312, 879, 1158
BtsIMutI CAGTG 6 cut(s) 312, 701, 879, 1128, 1158, 1186
Cac8I GCNNGC 6 cut(s) 216, 588, 899, 1068, 1126, 1231
CaiI CAGNNNCTG 1 cut(s) 357
Cfr10I RCCGGY 1 cut(s) 214
Cfr13I GGNCC 3 cut(s) 83, 458, 536
Csp6I GTAC 6 cut(s) 58, 354, 360, 797, 974, 1108
CspCI CAANNNNNGTGG 2 cut(s) 819, 854
CviQI GTAC 6 cut(s) 58, 354, 360, 797, 974, 1108
DdeI CTNAG 5 cut(s) 408, 641, 658, 1080, 1131
DpnI GATC 4 cut(s) 483, 525, 843, 1085
DpnII GATC 4 cut(s) 481, 523, 841, 1083
DraI TTTAAA 1 cut(s) 243
EaeI YGGCCR 3 cut(s) 895, 1278, 1287
Eam1104I CTCTTC 1 cut(s) 308
EarI CTCTTC 1 cut(s) 308
Eco130I CCWWGG 3 cut(s) 274, 684, 950
Eco47I GGWCC 3 cut(s) 83, 458, 536
Eco91I GGTNACC 1 cut(s) 7
EcoNI CCTNNNNNAGG 1 cut(s) 243
EcoO109I RGGNCCY 1 cut(s) 83
EcoO65I GGTNACC 1 cut(s) 7
EcoRII CCWGG 1 cut(s) 1289
EcoT14I CCWWGG 3 cut(s) 274, 684, 950
ErhI CCWWGG 3 cut(s) 274, 684, 950
FaqI GGGAC 2 cut(s) 608, 644
FauNDI CATATG 1 cut(s) 474
FokI GGATG 4 cut(s) 32, 428, 584, 620
FspBI CTAG 4 cut(s) 167, 219, 587, 1103
HaeIII GGCC 4 cut(s) 897, 1066, 1280, 1289
HapII CCGG 4 cut(s) 215, 581, 619, 1243
Hin1I GRCGYC 1 cut(s) 748
HindIII AAGCTT 2 cut(s) 285, 510
HinfI GANTC 7 cut(s) 194, 761, 878, 945, 1010, 1136, 1195
HpaII CCGG 4 cut(s) 215, 581, 619, 1243
HphI GGTGA 4 cut(s) 58, 225, 1168, 1267
Hpy166II GTNNAC 3 cut(s) 60, 93, 1019
Hpy188I TCNGA 4 cut(s) 462, 528, 1083, 1168
Hpy188III TCNNGA 7 cut(s) 158, 410, 765, 882, 932, 1103, 1238
Hpy8I GTNNAC 3 cut(s) 60, 93, 1019
Hpy99I CGWCG 1 cut(s) 753
HpyAV CCTTC 2 cut(s) 762, 816
HpyCH4III ACNGT 6 cut(s) 264, 353, 562, 924, 997, 1264
HpyCH4IV ACGT 3 cut(s) 748, 835, 1021
HpyF10VI GCNNNNNNNGC 5 cut(s) 211, 316, 968, 1076, 1286
HpyF3I CTNAG 5 cut(s) 408, 641, 658, 1080, 1131
HpySE526I ACGT 3 cut(s) 748, 835, 1021
Hsp92I GRCGYC 1 cut(s) 748
KroI GCCGGC 1 cut(s) 214
KroNI GCCGGC 1 cut(s) 216
Kzo9I GATC 4 cut(s) 481, 523, 841, 1083
LmnI GCTCC 3 cut(s) 28, 176, 583
LweI GCATC 3 cut(s) 10, 562, 637
MaeI CTAG 4 cut(s) 167, 219, 587, 1103
MaeII ACGT 3 cut(s) 748, 835, 1021
MaeIII GTNAC 4 cut(s) 7, 231, 784, 1156
MalI GATC 4 cut(s) 483, 525, 843, 1085
MboI GATC 4 cut(s) 481, 523, 841, 1083
MboII GAAGA 6 cut(s) 325, 400, 541, 709, 1270, 1324
MflI RGATCY 1 cut(s) 523
MhlI GDGCHC 1 cut(s) 671
MlsI TGGCCA 2 cut(s) 897, 1289
MluCI AATT 5 cut(s) 338, 753, 957, 1056, 1203
MluNI TGGCCA 2 cut(s) 897, 1289
MlyI GAGTC 2 cut(s) 770, 1019
MmeI TCCRAC 2 cut(s) 485, 518
Mox20I TGGCCA 2 cut(s) 897, 1289
MroNI GCCGGC 1 cut(s) 214
MroXI GAANNNNTTC 1 cut(s) 1057
MscI TGGCCA 2 cut(s) 897, 1289
MseI TTAA 3 cut(s) 242, 297, 623
MslI CAYNNNNRTG 1 cut(s) 891
Msp20I TGGCCA 2 cut(s) 897, 1289
MspA1I CMGCKG 2 cut(s) 901, 1124
MspI CCGG 4 cut(s) 215, 581, 619, 1243
MspR9I CCNGG 2 cut(s) 1244, 1291
Mva1269I GAATGC 1 cut(s) 864
MvaI CCWGG 1 cut(s) 1291
MwoI GCNNNNNNNGC 5 cut(s) 211, 316, 968, 1076, 1286
NaeI GCCGGC 1 cut(s) 216
NciI CCSGG 1 cut(s) 1244
NcoI CCATGG 2 cut(s) 684, 950
NdeI CATATG 1 cut(s) 474
NdeII GATC 4 cut(s) 481, 523, 841, 1083
NgoMIV GCCGGC 1 cut(s) 214
NheI GCTAGC 1 cut(s) 586
NlaIV GGNNCC 4 cut(s) 84, 172, 525, 682
NmeAIII GCCGAG 1 cut(s) 1042
NmuCI GTSAC 3 cut(s) 231, 784, 1156
NspI RCATGY 3 cut(s) 640, 912, 1233
PaeI GCATGC 1 cut(s) 1233
PcsI WCGNNNNNNNCGW 1 cut(s) 1278
PctI GAATGC 1 cut(s) 864
PdiI GCCGGC 1 cut(s) 216
PdmI GAANNNNTTC 1 cut(s) 1057
PfeI GAWTC 5 cut(s) 194, 878, 945, 1136, 1195
PleI GAGTC 2 cut(s) 769, 1018
PpsI GAGTC 2 cut(s) 769, 1018
Ppu21I YACGTR 1 cut(s) 1022
PpuMI RGGWCCY 1 cut(s) 83
PsiI TTATAA 1 cut(s) 1202
Psp5II RGGWCCY 1 cut(s) 83
Psp6I CCWGG 1 cut(s) 1289
PspEI GGTNACC 1 cut(s) 7
PspGI CCWGG 1 cut(s) 1289
PspN4I GGNNCC 4 cut(s) 84, 172, 525, 682
PspPI GGNCC 3 cut(s) 83, 458, 536
PspPPI RGGWCCY 1 cut(s) 83
PstI CTGCAG 1 cut(s) 1153
PstNI CAGNNNCTG 1 cut(s) 357
PsuI RGATCY 1 cut(s) 523
PvuII CAGCTG 1 cut(s) 901
RsaI GTAC 6 cut(s) 59, 355, 361, 798, 975, 1109
RsaNI GTAC 6 cut(s) 58, 354, 360, 797, 974, 1108
RseI CAYNNNNRTG 1 cut(s) 891
SaqAI TTAA 3 cut(s) 242, 297, 623
Sau3AI GATC 4 cut(s) 481, 523, 841, 1083
Sau96I GGNCC 3 cut(s) 83, 458, 536
SchI GAGTC 2 cut(s) 770, 1019
ScrFI CCNGG 2 cut(s) 1244, 1291
SduI GDGCHC 1 cut(s) 671
SfaNI GCATC 3 cut(s) 10, 562, 637
SfcI CTRYAG 1 cut(s) 1149
SfiI GGCCNNNNNGGCC 1 cut(s) 1286
SinI GGWCC 3 cut(s) 83, 458, 536
SmiMI CAYNNNNRTG 1 cut(s) 891
SmlI CTYRAG 1 cut(s) 853
SmoI CTYRAG 1 cut(s) 853
SphI GCATGC 1 cut(s) 1233
Sse9I AATT 5 cut(s) 338, 753, 957, 1056, 1203
SsiI CCGC 3 cut(s) 5, 26, 1122
SspI AATATT 1 cut(s) 1269
SspMI CTAG 4 cut(s) 167, 219, 587, 1103
StyD4I CCNGG 2 cut(s) 1242, 1289
StyI CCWWGG 3 cut(s) 274, 684, 950
TaaI ACNGT 6 cut(s) 264, 353, 562, 924, 997, 1264
TaiI ACGT 3 cut(s) 751, 838, 1024
TaqI TCGA 2 cut(s) 336, 751
TasI AATT 5 cut(s) 338, 753, 957, 1056, 1203
TatI WGTACW 2 cut(s) 57, 359
TfiI GAWTC 5 cut(s) 194, 878, 945, 1136, 1195
Tru1I TTAA 3 cut(s) 242, 297, 623
Tru9I TTAA 3 cut(s) 242, 297, 623
TscAI CASTG 6 cut(s) 312, 701, 879, 1135, 1158, 1186
TseFI GTSAC 3 cut(s) 231, 784, 1156
Tsp45I GTSAC 3 cut(s) 231, 784, 1156
TspDTI ATGAA 7 cut(s) 283, 297, 386, 395, 461, 917, 1128
TspGWI ACGGA 2 cut(s) 77, 1207
TspRI CASTG 6 cut(s) 312, 701, 879, 1135, 1158, 1186
VpaK11BI GGWCC 3 cut(s) 83, 458, 536
XagI CCTNNNNNAGG 1 cut(s) 243
XapI RAATTY 2 cut(s) 338, 753
XbaI TCTAGA 1 cut(s) 1102
XceI RCATGY 3 cut(s) 640, 912, 1233
XcmI CCANNNNNNNNNTGG 1 cut(s) 682
XmnI GAANNNNTTC 1 cut(s) 1057
XspI CTAG 4 cut(s) 167, 219, 587, 1103
ZraI GACGTC 1 cut(s) 749
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.