RchiOBHm_Chr7g0187601

START domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0003829
Physical Location & Seq
Reverse (-)
7381980 .. 7385648
3669 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ16747

Sequence Viewer

Length: 1281 bp
ATGGCTTTGGCGTCGGCTTTGCTGGAGATTCTGCGAAGACCCACGATTGTTGAAATGCTGGGGGAGCTCGTCAAGTTCATAACCCCTCTTTGGATTGCTGTGATTGTCGGGGTTTTCGTTGGGTGGGCGTGGCGGCCCAAATGGGTGACCGTGGTTGGTAGAGAATTGTTGGATAGCTCCACACAAAAGGAGTCATCTTCCTCATCTCCGCCCGCACCATCCACGACGTGTGGGATTTCGATTTCGAGTTTGAGTTCGTTGCTGCCGAATTGCATTCCTTGGGCTGCTGACGATAGAAATCTGAACCAGGCACCTACCACCAATAATGATTGCAGCTCACAGATTGAACAAGAAGAATCCTGTAATGTCTCCAATGAGGATCTACGCCACATATGTCAGCTTGTGGAGGAGAAAGATGGCGGTCCTGCGTGGATTCATATGATGGATCGGTCAACCCCAACTATGGGATATCAAGCATGGCGTAGGGATCCTCAGACTGGACCTCCACAATACCGAAGTAGAACTGTATTCGAAGATGCTTCACCTGAGACAGTGAGGGATTTCTTTTGGGATGATGAATTTCGTGCAAAGTGGGATGATATGCTTATTCATGCTTCTACTTTGGAAGAATGCCCGACTACTGGAACCATGCTAGTGCACTGGGTCCGCAAGTTTCCATTCTTTTGTAGCGACAGAGATTACACGATAGGCCGTCGCATTTGGGAATCGGGGCGATCATACTACTGTGTAACTAAGGGAGTTCCTTCCTCTGCTGTGCCAAGGCGTGACAAACCTAGACGTGTTGATCTGTACTATTCAAGTTGGTGCATACGTGCAGTTGAATCAAAAAGAGGGGATGGCCAACTTACTTCATGTGAGGTTTTACTGTTTCACCATGAAGATATGGGTATTCCATGGGAGATTGCGAAACTTGGTGTTCGTCAAGGTATGTGGGGAGCTGTTAAAAAGATTGAACCAGGCCTACGTTTATATCAAAAGGAAAGAGCCGCTGAAGCACCACTTTCGCGGTGTGCTAAAATGGCTCAGATCAACACGAAAGTCAGTGCTGATTACTTGAGATCGGTAGAAAACAGCACTAAGGATTCCATGGAGGTTGAAAATTCAGATTCAGCGGAGAAACCAGGAAGGAGGAATATACCTAAAGCTCTAGTTGTTGGAGGGGCAATAGTTCTTGCTTGCAGTCTTGATCGGGGCCTCTTGACTAAGGCAGTTATATTTGGAGTAGCTCGAAGATTTGCCAAGATCGGGAGGAGGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
START

Protein Analysis

426

Amino Acids

47.97

Weight (kDa)

8.39

Isoelectric Point (pI)

55.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
START PF01852 143 - 298 2e-08 START domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 310
AccII CGCG 1 cut(s) 1027
AciI CCGC 8 cut(s) 133, 209, 213, 420, 667, 1008, 1027, 1133
AclWI GGATC 4 cut(s) 387, 453, 482, 495
AcoI YGGCCR 1 cut(s) 859
AcsI RAATTY 2 cut(s) 578, 1120
AcuI CTGAAG 1 cut(s) 1032
AcyI GRCGYC 1 cut(s) 11
AdeI CACNNNGTG 1 cut(s) 228
AfaI GTAC 1 cut(s) 812
AfiI CCNNNNNNNGG 6 cut(s) 90, 463, 464, 497, 641, 1266
AflIII ACRYGT 2 cut(s) 227, 799
AgsI TTSAA 6 cut(s) 53, 347, 819, 842, 974, 1118
AjiI CACGTC 2 cut(s) 228, 800
AjnI CCWGG 3 cut(s) 306, 976, 1141
AluBI AGCT 7 cut(s) 67, 177, 336, 400, 959, 1166, 1247
AluI AGCT 7 cut(s) 67, 177, 336, 400, 959, 1166, 1247
Alw21I GWGCWC 2 cut(s) 69, 660
Alw26I GTCTC 2 cut(s) 373, 542
Alw44I GTGCAC 1 cut(s) 656
AlwI GGATC 4 cut(s) 387, 453, 482, 495
AoxI GGCC 5 cut(s) 134, 709, 859, 979, 1213
ApaLI GTGCAC 1 cut(s) 656
ApeKI GCWGC 3 cut(s) 262, 284, 333
ApoI RAATTY 2 cut(s) 578, 1120
AspS9I GGNCC 5 cut(s) 135, 422, 500, 664, 1213
AsuHPI GGTGA 3 cut(s) 157, 534, 884
AsuII TTCGAA 1 cut(s) 531
AvaII GGWCC 3 cut(s) 422, 500, 664
BaeGI GKGCMC 1 cut(s) 660
BalI TGGCCA 1 cut(s) 861
BamHI GGATCC 1 cut(s) 487
BanI GGYRCC 1 cut(s) 310
BanII GRGCYC 1 cut(s) 69
BbsI GAAGAC 1 cut(s) 43
Bbv12I GWGCWC 2 cut(s) 69, 660
BbvI GCAGC 3 cut(s) 249, 271, 345
BccI CCATC 4 cut(s) 226, 410, 436, 851
BceAI ACGGC 1 cut(s) 696
BcgI CGANNNNNNTGC 2 cut(s) 1005, 1039
BciT130I CCWGG 3 cut(s) 308, 978, 1143
BcoDI GTCTC 2 cut(s) 373, 542
BfaI CTAG 3 cut(s) 653, 795, 1169
BisI GCNGC 5 cut(s) 134, 263, 285, 334, 1008
BlsI GCNGC 5 cut(s) 135, 264, 286, 335, 1009
Bme1390I CCNGG 3 cut(s) 308, 978, 1143
Bme18I GGWCC 3 cut(s) 422, 500, 664
BmgBI CACGTC 2 cut(s) 228, 800
BmgT120I GGNCC 5 cut(s) 135, 422, 500, 664, 1213
BmiI GGNNCC 5 cut(s) 312, 489, 646, 665, 1214
BmrFI CCNGG 3 cut(s) 308, 978, 1143
BmrI ACTGGG 1 cut(s) 670
BmsI GCATC 1 cut(s) 526
BmuI ACTGGG 1 cut(s) 670
BpiI GAAGAC 1 cut(s) 43
BpmI CTGGAG 1 cut(s) 44
Bpu14I TTCGAA 1 cut(s) 531
BpuEI CTTGAG 1 cut(s) 1096
BsaAI YACGTR 1 cut(s) 833
BsaBI GATNNNNATC 1 cut(s) 297
BsaHI GRCGYC 1 cut(s) 11
BsaJI CCNNGG 5 cut(s) 150, 278, 779, 914, 1107
BsaXI ACNNNNNCTCC 2 cut(s) 487, 517
Bsc4I CCNNNNNNNGG 6 cut(s) 90, 463, 464, 497, 641, 1266
Bse1I ACTGG 3 cut(s) 502, 646, 665
Bse8I GATNNNNATC 1 cut(s) 297
BseBI CCWGG 3 cut(s) 308, 978, 1143
BseDI CCNNGG 5 cut(s) 150, 278, 779, 914, 1107
BseGI GGATG 4 cut(s) 218, 577, 601, 862
BseJI GATNNNNATC 1 cut(s) 297
BseLI CCNNNNNNNGG 6 cut(s) 90, 463, 464, 497, 641, 1266
BseMII CTCAG 3 cut(s) 506, 537, 1058
BseNI ACTGG 3 cut(s) 502, 646, 665
BseRI GAGGAG 1 cut(s) 422
BseSI GKGCMC 1 cut(s) 660
BseXI GCAGC 3 cut(s) 249, 271, 345
BseYI CCCAGC 1 cut(s) 58
BsgI GTGCAG 1 cut(s) 855
Bsh1236I CGCG 1 cut(s) 1027
BshFI GGCC 5 cut(s) 136, 711, 861, 981, 1215
BshNI GGYRCC 1 cut(s) 310
BsiHKAI GWGCWC 2 cut(s) 69, 660
BslI CCNNNNNNNGG 6 cut(s) 90, 463, 464, 497, 641, 1266
BsmAI GTCTC 2 cut(s) 373, 542
BsmI GAATGC 2 cut(s) 273, 635
BsnI GGCC 5 cut(s) 136, 711, 861, 981, 1215
Bsp119I TTCGAA 1 cut(s) 531
Bsp1286I GDGCHC 2 cut(s) 69, 660
Bsp143I GATC 9 cut(s) 379, 445, 487, 734, 805, 1047, 1079, 1207, 1263
Bsp19I CCATGG 2 cut(s) 914, 1107
BspACI CCGC 8 cut(s) 133, 209, 213, 420, 667, 1008, 1027, 1133
BspANI GGCC 5 cut(s) 136, 711, 861, 981, 1215
BspCNI CTCAG 3 cut(s) 505, 538, 1057
BspFNI CGCG 1 cut(s) 1027
BspLI GGNNCC 5 cut(s) 312, 489, 646, 665, 1214
BspPI GGATC 4 cut(s) 387, 453, 482, 495
BspT104I TTCGAA 1 cut(s) 531
BspT107I GGYRCC 1 cut(s) 310
BsrI ACTGG 3 cut(s) 502, 646, 665
BssECI CCNNGG 5 cut(s) 150, 278, 779, 914, 1107
BssMI GATC 9 cut(s) 379, 445, 487, 734, 805, 1047, 1079, 1207, 1263
BssNI GRCGYC 1 cut(s) 11
BssT1I CCWWGG 4 cut(s) 278, 779, 914, 1107
Bst2UI CCWGG 3 cut(s) 308, 978, 1143
Bst4CI ACNGT 5 cut(s) 151, 526, 553, 746, 888
BstACI GRCGYC 1 cut(s) 11
BstBAI YACGTR 1 cut(s) 833
BstBI TTCGAA 1 cut(s) 531
BstC8I GCNNGC 2 cut(s) 213, 1198
BstDEI CTNAG 6 cut(s) 492, 546, 753, 1044, 1098, 1224
BstDSI CCRYGG 3 cut(s) 150, 914, 1107
BstEII GGTNACC 1 cut(s) 145
BstF5I GGATG 4 cut(s) 218, 577, 601, 862
BstFNI CGCG 1 cut(s) 1027
BstKTI GATC 9 cut(s) 382, 448, 490, 737, 808, 1050, 1082, 1210, 1266
BstMAI GTCTC 2 cut(s) 373, 542
BstMBI GATC 9 cut(s) 379, 445, 487, 734, 805, 1047, 1079, 1207, 1263
BstMWI GCNNNNNNNGC 3 cut(s) 64, 1013, 1040
BstNI CCWGG 3 cut(s) 308, 978, 1143
BstPI GGTNACC 1 cut(s) 145
BstSCI CCNGG 3 cut(s) 306, 976, 1141
BstSLI GKGCMC 1 cut(s) 660
BstUI CGCG 1 cut(s) 1027
BstV1I GCAGC 3 cut(s) 249, 271, 345
BstV2I GAAGAC 1 cut(s) 43
BstX2I RGATCY 2 cut(s) 379, 487
BstYI RGATCY 2 cut(s) 379, 487
BsuRI GGCC 5 cut(s) 136, 711, 861, 981, 1215
BtgI CCRYGG 3 cut(s) 150, 914, 1107
BtrI CACGTC 2 cut(s) 228, 800
BtsCI GGATG 4 cut(s) 218, 577, 601, 862
BtsIMutI CAGTG 3 cut(s) 558, 658, 1069
Cac8I GCNNGC 2 cut(s) 213, 1198
Cfr13I GGNCC 5 cut(s) 135, 422, 500, 664, 1213
Csp6I GTAC 1 cut(s) 811
CspCI CAANNNNNGTGG 4 cut(s) 31, 66, 932, 967
CviAII CATG 7 cut(s) 477, 611, 649, 873, 896, 915, 1108
CviQI GTAC 1 cut(s) 811
DdeI CTNAG 6 cut(s) 492, 546, 753, 1044, 1098, 1224
DpnI GATC 9 cut(s) 381, 447, 489, 736, 807, 1049, 1081, 1209, 1265
DpnII GATC 9 cut(s) 379, 445, 487, 734, 805, 1047, 1079, 1207, 1263
DraIII CACNNNGTG 1 cut(s) 228
EaeI YGGCCR 1 cut(s) 859
EciI GGCGGA 1 cut(s) 198
Ecl136II GAGCTC 1 cut(s) 67
Eco130I CCWWGG 4 cut(s) 278, 779, 914, 1107
Eco147I AGGCCT 1 cut(s) 981
Eco24I GRGCYC 1 cut(s) 69
Eco32I GATATC 1 cut(s) 470
Eco47I GGWCC 3 cut(s) 422, 500, 664
Eco53kI GAGCTC 1 cut(s) 67
Eco57I CTGAAG 1 cut(s) 1032
Eco91I GGTNACC 1 cut(s) 145
EcoICRI GAGCTC 1 cut(s) 67
EcoO109I RGGNCCY 1 cut(s) 1213
EcoO65I GGTNACC 1 cut(s) 145
EcoRII CCWGG 3 cut(s) 306, 976, 1141
EcoRV GATATC 1 cut(s) 470
EcoT14I CCWWGG 4 cut(s) 278, 779, 914, 1107
EcoT38I GRGCYC 1 cut(s) 69
ErhI CCWWGG 4 cut(s) 278, 779, 914, 1107
FaeI CATG 7 cut(s) 480, 614, 652, 876, 899, 918, 1111
FalI AAGNNNNNCTT 2 cut(s) 1005, 1037
FatI CATG 7 cut(s) 476, 610, 648, 872, 895, 914, 1107
FauI CCCGC 1 cut(s) 220
FauNDI CATATG 2 cut(s) 392, 438
Fnu4HI GCNGC 5 cut(s) 134, 263, 285, 334, 1008
FokI GGATG 4 cut(s) 205, 584, 608, 869
FriOI GRGCYC 1 cut(s) 69
Fsp4HI GCNGC 5 cut(s) 134, 263, 285, 334, 1008
FspBI CTAG 3 cut(s) 653, 795, 1169
GluI GCNGC 5 cut(s) 134, 263, 285, 334, 1008
GsaI CCCAGC 1 cut(s) 62
GsuI CTGGAG 1 cut(s) 44
HaeIII GGCC 5 cut(s) 136, 711, 861, 981, 1215
Hin1I GRCGYC 1 cut(s) 11
Hin1II CATG 7 cut(s) 480, 614, 652, 876, 899, 918, 1111
HincII GTYRAC 1 cut(s) 453
HindII GTYRAC 1 cut(s) 453
HinfI GANTC 8 cut(s) 28, 191, 356, 433, 725, 842, 1103, 1127
HphI GGTGA 3 cut(s) 157, 534, 884
Hpy166II GTNNAC 2 cut(s) 453, 658
Hpy188I TCNGA 4 cut(s) 303, 495, 1047, 1126
Hpy188III TCNNGA 3 cut(s) 1205, 1219, 1267
Hpy8I GTNNAC 2 cut(s) 453, 658
Hpy99I CGWCG 3 cut(s) 16, 229, 717
HpyAV CCTTC 2 cut(s) 774, 1140
HpyCH4III ACNGT 5 cut(s) 151, 526, 553, 746, 888
HpyCH4IV ACGT 4 cut(s) 227, 799, 832, 985
HpyCH4V TGCA 7 cut(s) 273, 333, 587, 658, 828, 836, 1200
HpyF10VI GCNNNNNNNGC 3 cut(s) 64, 1013, 1040
HpyF3I CTNAG 6 cut(s) 492, 546, 753, 1044, 1098, 1224
HpySE526I ACGT 4 cut(s) 227, 799, 832, 985
Hsp92I GRCGYC 1 cut(s) 11
Hsp92II CATG 7 cut(s) 480, 614, 652, 876, 899, 918, 1111
Kzo9I GATC 9 cut(s) 379, 445, 487, 734, 805, 1047, 1079, 1207, 1263
LmnI GCTCC 3 cut(s) 64, 182, 956
Lsp1109I GCAGC 3 cut(s) 249, 271, 345
LweI GCATC 1 cut(s) 526
MaeI CTAG 3 cut(s) 653, 795, 1169
MaeII ACGT 4 cut(s) 227, 799, 832, 985
MaeIII GTNAC 3 cut(s) 145, 748, 785
MalI GATC 9 cut(s) 381, 447, 489, 736, 807, 1049, 1081, 1209, 1265
MboI GATC 9 cut(s) 379, 445, 487, 734, 805, 1047, 1079, 1207, 1263
MboII GAAGA 7 cut(s) 48, 189, 365, 545, 638, 911, 1263
MflI RGATCY 2 cut(s) 379, 487
MhlI GDGCHC 2 cut(s) 69, 660
MlsI TGGCCA 1 cut(s) 861
MluCI AATT 4 cut(s) 164, 268, 578, 1120
MluNI TGGCCA 1 cut(s) 861
MlyI GAGTC 1 cut(s) 200
MmeI TCCRAC 2 cut(s) 150, 1156
Mox20I TGGCCA 1 cut(s) 861
MscI TGGCCA 1 cut(s) 861
MseI TTAA 1 cut(s) 963
MslI CAYNNNNRTG 1 cut(s) 653
Msp20I TGGCCA 1 cut(s) 861
MspA1I CMGCKG 2 cut(s) 1010, 1133
MspR9I CCNGG 3 cut(s) 308, 978, 1143
Mva1269I GAATGC 2 cut(s) 273, 635
MvaI CCWGG 3 cut(s) 308, 978, 1143
MvnI CGCG 1 cut(s) 1027
MwoI GCNNNNNNNGC 3 cut(s) 64, 1013, 1040
NcoI CCATGG 2 cut(s) 914, 1107
NdeI CATATG 2 cut(s) 392, 438
NdeII GATC 9 cut(s) 379, 445, 487, 734, 805, 1047, 1079, 1207, 1263
NlaIII CATG 7 cut(s) 480, 614, 652, 876, 899, 918, 1111
NlaIV GGNNCC 5 cut(s) 312, 489, 646, 665, 1214
NmuCI GTSAC 2 cut(s) 145, 785
NspV TTCGAA 1 cut(s) 531
PceI AGGCCT 1 cut(s) 981
PcsI WCGNNNNNNNCGW 2 cut(s) 114, 263
PctI GAATGC 2 cut(s) 273, 635
PfeI GAWTC 7 cut(s) 28, 356, 433, 725, 842, 1103, 1127
PkrI GCNGC 5 cut(s) 135, 264, 286, 335, 1009
PleI GAGTC 1 cut(s) 199
PpsI GAGTC 1 cut(s) 199
Ppu21I YACGTR 1 cut(s) 833
Psp124BI GAGCTC 1 cut(s) 69
Psp6I CCWGG 3 cut(s) 306, 976, 1141
PspEI GGTNACC 1 cut(s) 145
PspFI CCCAGC 1 cut(s) 58
PspGI CCWGG 3 cut(s) 306, 976, 1141
PspN4I GGNNCC 5 cut(s) 312, 489, 646, 665, 1214
PspPI GGNCC 5 cut(s) 135, 422, 500, 664, 1213
PsrI GAACNNNNNNTAC 2 cut(s) 966, 998
PsuI RGATCY 2 cut(s) 379, 487
RsaI GTAC 1 cut(s) 812
RsaNI GTAC 1 cut(s) 811
RseI CAYNNNNRTG 1 cut(s) 653
SacI GAGCTC 1 cut(s) 69
SaqAI TTAA 1 cut(s) 963
SatI GCNGC 5 cut(s) 134, 263, 285, 334, 1008
Sau3AI GATC 9 cut(s) 379, 445, 487, 734, 805, 1047, 1079, 1207, 1263
Sau96I GGNCC 5 cut(s) 135, 422, 500, 664, 1213
SchI GAGTC 1 cut(s) 200
ScrFI CCNGG 3 cut(s) 308, 978, 1143
SduI GDGCHC 2 cut(s) 69, 660
SfaNI GCATC 1 cut(s) 526
SfuI TTCGAA 1 cut(s) 531
SinI GGWCC 3 cut(s) 422, 500, 664
SmiMI CAYNNNNRTG 1 cut(s) 653
SmlI CTYRAG 1 cut(s) 1075
SmoI CTYRAG 1 cut(s) 1075
Sse9I AATT 4 cut(s) 164, 268, 578, 1120
SseBI AGGCCT 1 cut(s) 981
SsiI CCGC 8 cut(s) 133, 209, 213, 420, 667, 1008, 1027, 1133
SspMI CTAG 3 cut(s) 653, 795, 1169
SstI GAGCTC 1 cut(s) 69
StuI AGGCCT 1 cut(s) 981
StyD4I CCNGG 3 cut(s) 306, 976, 1141
StyI CCWWGG 4 cut(s) 278, 779, 914, 1107
TaaI ACNGT 5 cut(s) 151, 526, 553, 746, 888
TaiI ACGT 4 cut(s) 230, 802, 835, 988
TaqI TCGA 4 cut(s) 239, 245, 531, 1249
TaqII GACCGA 1 cut(s) 438
TasI AATT 4 cut(s) 164, 268, 578, 1120
TatI WGTACW 1 cut(s) 810
TauI GCSGC 2 cut(s) 136, 1010
TfiI GAWTC 7 cut(s) 28, 356, 433, 725, 842, 1103, 1127
Tru1I TTAA 1 cut(s) 963
Tru9I TTAA 1 cut(s) 963
TscAI CASTG 3 cut(s) 558, 665, 1069
TseFI GTSAC 2 cut(s) 145, 785
TseI GCWGC 3 cut(s) 262, 284, 333
Tsp45I GTSAC 2 cut(s) 145, 785
TspDTI ATGAA 6 cut(s) 67, 425, 591, 599, 861, 912
TspRI CASTG 3 cut(s) 558, 665, 1069
VneI GTGCAC 1 cut(s) 656
VpaK11BI GGWCC 3 cut(s) 422, 500, 664
XapI RAATTY 2 cut(s) 578, 1120
XspI CTAG 3 cut(s) 653, 795, 1169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.