Rroxscaffold_4G00277860

START domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0003829
Physical Location & Seq
Forward (+)
1098321 .. 1100991
2671 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00277860.1

Sequence Viewer

Length: 1323 bp
ATGGCGGTAACCAACATGGGCCCTCTGCATGAGATTTTGGAGATGATGAAAATAGAGTACACGGACTTTGTGGTGTTTCTGGGTCCTCTGTGGACGGCTTTCCTTATTGGTGTTGTGATTGGTTGGGCATGGAAGCCGAAGTGGGCAACATTTGGTCAGGAAAAACTAGGCTCCTCTGTTTCTAAAGTCTTGGATTCGTCTATGCCTACTTCGCCGGCTAGGTCTGTGATGTCACCACCTCTAAAAGGGTTCTCATCAGCACCGTGCTTGTTCCAAGGCTATGAAGCTTGGTTCATTAACCATGCAGTGCAGAAGAGGCTTTCTCCCTCACCTATCGAATTTGATGACCACAGTACCTGTACATCGCAGATAAATGAGAAGAAGTCTAATGTTGTGGCTCAGGAGGATGTAGAAAATCTATGTCGGCTTGTAGATATGAAAGATGGAGGTCCGGCTTGGATACATATGATGGATCGTTCTACTCCAACTATGGCCTGCCAAGCTTGGCGTAGGGATCCAAAGATTGGTCCTCCTCAATATCGTAGCAGGACTGTGTATGAGGATGCAACGCCGGAGCTAGTGAGGGACTTTTTCTGGGATGATGAGTTCCGGTTAAGGTGGGACGACATGCTTAGTGATGCCACAACCTTAGATGAGTGCCCAACCACAGGAACCATGGTTGTTCAGTGGATAAAGAAGTTCCCGTTCTTCTGTAAAGACAGGGAATACATAATAGGACGTCGAATTTGGGAGTCAGGAAAGAAGTATTATTGCGTGACAAAGGGAGTACCTTATCCTTCTATTCAAAGGCATGACAAACCAAGACGTGTGGATCACTACTACTCAAGTTGGTGCATTCAAGCAGTGGAATCAAGACACGGCAATGGCCAGCTGAGTGCATGTGAAGTTCAACTGTTTCATCAAGAGGAAACAGGAATCCCATGGGAAATTGCAAAACTCGCTGTACGAAAAGGAATGTGGGATACTGTAAAGAACACAGAGTCTGGTTTACGTGCTTACCAGAAAGAGAGAGCGTTAGGTGGAATAATTTCTCGGCCTGCCTTTCTGGCTCAGATCAACACCAAAATAAATCTAGAGTACCTGAAAGCCTCCGCTGGCACTGAGGATTCATCACAAACTGCAGTGGTGACTACATCTGACAAACCTTTCAGTGGGAACATACGGAATCTTATAATTGTTGGTGGGAGTATTCTCCTTGCATGCACTCTTGACCGGGGGTTTCTGGTGAAGACAGTAATATTCGGCGTGGCTGATGTGGCCAGGAAGTTTGCAAATGTGGGGAAGAAGTCAAAACCAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
START

Protein Analysis

440

Amino Acids

49.78

Weight (kDa)

8.76

Isoelectric Point (pI)

46.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
START PF01852 179 - 338 5e-07 START domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1193
AatII GACGTC 1 cut(s) 742
AccB7I CCANNNNNTGG 1 cut(s) 524
AciI CCGC 2 cut(s) 5, 1113
AclWI GGATC 4 cut(s) 480, 509, 522, 840
AcoI YGGCCR 2 cut(s) 886, 1278
AcsI RAATTY 2 cut(s) 338, 744
AcyI GRCGYC 1 cut(s) 739
AfaI GTAC 6 cut(s) 59, 355, 361, 789, 966, 1100
AfiI CCNNNNNNNGG 4 cut(s) 245, 524, 668, 1172
AflIII ACRYGT 1 cut(s) 826
AgsI TTSAA 3 cut(s) 806, 860, 911
AjiI CACGTC 1 cut(s) 827
AjnI CCWGG 1 cut(s) 1280
AloI GAACNNNNNNTCC 2 cut(s) 590, 622
AluBI AGCT 4 cut(s) 287, 503, 577, 892
AluI AGCT 4 cut(s) 287, 503, 577, 892
AlwI GGATC 4 cut(s) 480, 509, 522, 840
AlwNI CAGNNNCTG 2 cut(s) 357, 1004
AoxI GGCC 5 cut(s) 19, 492, 886, 1055, 1278
ApaI GGGCCC 1 cut(s) 23
ApoI RAATTY 2 cut(s) 338, 744
ArsI GACNNNNNNTTYG 2 cut(s) 729, 761
Asp700I GAANNNNTTC 1 cut(s) 1048
AspS9I GGNCC 5 cut(s) 19, 20, 83, 449, 527
AsuC2I CCSGG 1 cut(s) 1235
AsuHPI GGTGA 4 cut(s) 225, 321, 1159, 1258
AvaII GGWCC 3 cut(s) 83, 449, 527
BaeGI GKGCMC 2 cut(s) 23, 662
BaeI ACNNNNGTAYC 2 cut(s) 337, 370
BalI TGGCCA 2 cut(s) 888, 1280
BamHI GGATCC 1 cut(s) 514
BanII GRGCYC 1 cut(s) 23
BbsI GAAGAC 1 cut(s) 1256
BccI CCATC 2 cut(s) 437, 463
BceAI ACGGC 2 cut(s) 111, 895
BciT130I CCWGG 1 cut(s) 1282
BciVI GTATCC 2 cut(s) 453, 976
BcnI CCSGG 1 cut(s) 1235
BfaI CTAG 4 cut(s) 167, 219, 578, 1094
BfmI CTRYAG 1 cut(s) 1140
BfuI GTATCC 2 cut(s) 453, 976
BglI GCCNNNNNGGC 1 cut(s) 1067
Bme1390I CCNGG 2 cut(s) 1235, 1282
Bme18I GGWCC 3 cut(s) 83, 449, 527
BmgBI CACGTC 1 cut(s) 827
BmgT120I GGNCC 5 cut(s) 19, 20, 83, 449, 527
BmiI GGNNCC 5 cut(s) 21, 84, 172, 516, 673
BmrFI CCNGG 2 cut(s) 1235, 1282
BmsI GCATC 2 cut(s) 553, 628
BpiI GAAGAC 1 cut(s) 1256
BplI GAGNNNNNCTC 2 cut(s) 307, 339
Bpu10I CCTNAGC 1 cut(s) 399
BpuEI CTTGAG 1 cut(s) 829
BpuMI CCSGG 1 cut(s) 1235
BsaAI YACGTR 1 cut(s) 1013
BsaHI GRCGYC 1 cut(s) 739
BsaJI CCNNGG 4 cut(s) 274, 675, 941, 1234
BsaWI WCCGGW 1 cut(s) 609
Bsc4I CCNNNNNNNGG 4 cut(s) 245, 524, 668, 1172
Bse118I RCCGGY 1 cut(s) 214
Bse3DI GCAATG 1 cut(s) 889
BseBI CCWGG 1 cut(s) 1282
BseDI CCNNGG 4 cut(s) 274, 675, 941, 1234
BseGI GGATG 3 cut(s) 412, 568, 604
BseLI CCNNNNNNNGG 4 cut(s) 245, 524, 668, 1172
BseMI GCAATG 1 cut(s) 889
BseMII CTCAG 4 cut(s) 413, 884, 1085, 1113
BseRI GAGGAG 2 cut(s) 163, 522
BseSI GKGCMC 2 cut(s) 23, 662
BsgI GTGCAG 1 cut(s) 329
BshFI GGCC 5 cut(s) 21, 494, 888, 1057, 1280
BsiSI CCGG 5 cut(s) 215, 452, 572, 610, 1234
BslFI GGGAC 2 cut(s) 599, 635
BslI CCNNNNNNNGG 4 cut(s) 245, 524, 668, 1172
BsmFI GGGAC 2 cut(s) 599, 635
BsmI GAATGC 1 cut(s) 855
BsnI GGCC 5 cut(s) 21, 494, 888, 1057, 1280
Bsp120I GGGCCC 1 cut(s) 19
Bsp1286I GDGCHC 2 cut(s) 23, 662
Bsp1407I TGTACA 1 cut(s) 359
Bsp143I GATC 4 cut(s) 472, 514, 832, 1074
Bsp19I CCATGG 2 cut(s) 675, 941
BspACI CCGC 2 cut(s) 5, 1113
BspANI GGCC 5 cut(s) 21, 494, 888, 1057, 1280
BspCNI CTCAG 4 cut(s) 412, 885, 1084, 1114
BspLI GGNNCC 5 cut(s) 21, 84, 172, 516, 673
BspMAI CTGCAG 1 cut(s) 1144
BspPI GGATC 4 cut(s) 480, 509, 522, 840
BsrDI GCAATG 1 cut(s) 889
BsrFI RCCGGY 1 cut(s) 214
BsrGI TGTACA 1 cut(s) 359
BssAI RCCGGY 1 cut(s) 214
BssECI CCNNGG 4 cut(s) 274, 675, 941, 1234
BssMI GATC 4 cut(s) 472, 514, 832, 1074
BssNI GRCGYC 1 cut(s) 739
BssT1I CCWWGG 3 cut(s) 274, 675, 941
Bst2UI CCWGG 1 cut(s) 1282
Bst4CI ACNGT 6 cut(s) 264, 353, 553, 915, 988, 1255
Bst6I CTCTTC 1 cut(s) 308
BstACI GRCGYC 1 cut(s) 739
BstAUI TGTACA 1 cut(s) 359
BstBAI YACGTR 1 cut(s) 1013
BstC8I GCNNGC 6 cut(s) 216, 496, 890, 1059, 1117, 1222
BstDEI CTNAG 6 cut(s) 399, 632, 649, 893, 1071, 1122
BstDSI CCRYGG 2 cut(s) 675, 941
BstEII GGTNACC 1 cut(s) 7
BstENI CCTNNNNNAGG 1 cut(s) 243
BstF5I GGATG 3 cut(s) 412, 568, 604
BstKTI GATC 4 cut(s) 475, 517, 835, 1077
BstMBI GATC 4 cut(s) 472, 514, 832, 1074
BstMWI GCNNNNNNNGC 6 cut(s) 211, 316, 500, 959, 1067, 1277
BstNI CCWGG 1 cut(s) 1282
BstNSI RCATGY 3 cut(s) 631, 903, 1224
BstPI GGTNACC 1 cut(s) 7
BstSCI CCNGG 2 cut(s) 1233, 1280
BstSFI CTRYAG 1 cut(s) 1140
BstSLI GKGCMC 2 cut(s) 23, 662
BstV2I GAAGAC 1 cut(s) 1256
BstX2I RGATCY 1 cut(s) 514
BstYI RGATCY 1 cut(s) 514
BsuI GTATCC 2 cut(s) 453, 976
BsuRI GGCC 5 cut(s) 21, 494, 888, 1057, 1280
BtgI CCRYGG 2 cut(s) 675, 941
BtgZI GCGATG 1 cut(s) 348
BtrI CACGTC 1 cut(s) 827
BtsCI GGATG 3 cut(s) 412, 568, 604
BtsI GCAGTG 3 cut(s) 312, 870, 1149
BtsIMutI CAGTG 6 cut(s) 312, 692, 870, 1119, 1149, 1177
Cac8I GCNNGC 6 cut(s) 216, 496, 890, 1059, 1117, 1222
CaiI CAGNNNCTG 2 cut(s) 357, 1004
Cfr10I RCCGGY 1 cut(s) 214
Cfr13I GGNCC 5 cut(s) 19, 20, 83, 449, 527
Csp6I GTAC 6 cut(s) 58, 354, 360, 788, 965, 1099
CspCI CAANNNNNGTGG 2 cut(s) 810, 845
CviQI GTAC 6 cut(s) 58, 354, 360, 788, 965, 1099
DdeI CTNAG 6 cut(s) 399, 632, 649, 893, 1071, 1122
DpnI GATC 4 cut(s) 474, 516, 834, 1076
DpnII GATC 4 cut(s) 472, 514, 832, 1074
EaeI YGGCCR 2 cut(s) 886, 1278
Eam1104I CTCTTC 1 cut(s) 308
EarI CTCTTC 1 cut(s) 308
Eco130I CCWWGG 3 cut(s) 274, 675, 941
Eco24I GRGCYC 1 cut(s) 23
Eco47I GGWCC 3 cut(s) 83, 449, 527
Eco91I GGTNACC 1 cut(s) 7
EcoNI CCTNNNNNAGG 1 cut(s) 243
EcoO109I RGGNCCY 2 cut(s) 20, 83
EcoO65I GGTNACC 1 cut(s) 7
EcoRII CCWGG 1 cut(s) 1280
EcoT14I CCWWGG 3 cut(s) 274, 675, 941
EcoT38I GRGCYC 1 cut(s) 23
ErhI CCWWGG 3 cut(s) 274, 675, 941
FaqI GGGAC 2 cut(s) 599, 635
FauNDI CATATG 1 cut(s) 465
FokI GGATG 3 cut(s) 419, 575, 611
FriOI GRGCYC 1 cut(s) 23
FspBI CTAG 4 cut(s) 167, 219, 578, 1094
HaeIII GGCC 5 cut(s) 21, 494, 888, 1057, 1280
HapII CCGG 5 cut(s) 215, 452, 572, 610, 1234
Hin1I GRCGYC 1 cut(s) 739
HindIII AAGCTT 2 cut(s) 285, 501
HinfI GANTC 7 cut(s) 194, 752, 869, 936, 1001, 1127, 1186
HpaII CCGG 5 cut(s) 215, 452, 572, 610, 1234
HphI GGTGA 4 cut(s) 225, 321, 1159, 1258
Hpy166II GTNNAC 3 cut(s) 60, 93, 1010
Hpy188I TCNGA 2 cut(s) 1074, 1159
Hpy188III TCNNGA 7 cut(s) 158, 401, 756, 873, 923, 1094, 1229
Hpy8I GTNNAC 3 cut(s) 60, 93, 1010
Hpy99I CGWCG 1 cut(s) 744
HpyAV CCTTC 1 cut(s) 807
HpyCH4III ACNGT 6 cut(s) 264, 353, 553, 915, 988, 1255
HpyCH4IV ACGT 3 cut(s) 739, 826, 1012
HpyF10VI GCNNNNNNNGC 6 cut(s) 211, 316, 500, 959, 1067, 1277
HpyF3I CTNAG 6 cut(s) 399, 632, 649, 893, 1071, 1122
HpySE526I ACGT 3 cut(s) 739, 826, 1012
Hsp92I GRCGYC 1 cut(s) 739
KroI GCCGGC 1 cut(s) 214
KroNI GCCGGC 1 cut(s) 216
Kzo9I GATC 4 cut(s) 472, 514, 832, 1074
LmnI GCTCC 2 cut(s) 176, 574
LweI GCATC 2 cut(s) 553, 628
MaeI CTAG 4 cut(s) 167, 219, 578, 1094
MaeII ACGT 3 cut(s) 739, 826, 1012
MaeIII GTNAC 4 cut(s) 7, 231, 775, 1147
MalI GATC 4 cut(s) 474, 516, 834, 1076
MboI GATC 4 cut(s) 472, 514, 832, 1074
MboII GAAGA 5 cut(s) 325, 391, 700, 1261, 1315
MflI RGATCY 1 cut(s) 514
MhlI GDGCHC 2 cut(s) 23, 662
MlsI TGGCCA 2 cut(s) 888, 1280
MluCI AATT 5 cut(s) 338, 744, 948, 1047, 1194
MluNI TGGCCA 2 cut(s) 888, 1280
MlyI GAGTC 2 cut(s) 761, 1010
MmeI TCCRAC 1 cut(s) 509
Mox20I TGGCCA 2 cut(s) 888, 1280
MroNI GCCGGC 1 cut(s) 214
MroXI GAANNNNTTC 1 cut(s) 1048
MscI TGGCCA 2 cut(s) 888, 1280
MseI TTAA 2 cut(s) 297, 614
MslI CAYNNNNRTG 1 cut(s) 882
Msp20I TGGCCA 2 cut(s) 888, 1280
MspA1I CMGCKG 2 cut(s) 892, 1115
MspI CCGG 5 cut(s) 215, 452, 572, 610, 1234
MspR9I CCNGG 2 cut(s) 1235, 1282
Mva1269I GAATGC 1 cut(s) 855
MvaI CCWGG 1 cut(s) 1282
MwoI GCNNNNNNNGC 6 cut(s) 211, 316, 500, 959, 1067, 1277
NaeI GCCGGC 1 cut(s) 216
NciI CCSGG 1 cut(s) 1235
NcoI CCATGG 2 cut(s) 675, 941
NdeI CATATG 1 cut(s) 465
NdeII GATC 4 cut(s) 472, 514, 832, 1074
NgoMIV GCCGGC 1 cut(s) 214
NlaIV GGNNCC 5 cut(s) 21, 84, 172, 516, 673
NmeAIII GCCGAG 1 cut(s) 1033
NmuCI GTSAC 3 cut(s) 231, 775, 1147
NspI RCATGY 3 cut(s) 631, 903, 1224
PaeI GCATGC 1 cut(s) 1224
PctI GAATGC 1 cut(s) 855
PdiI GCCGGC 1 cut(s) 216
PdmI GAANNNNTTC 1 cut(s) 1048
PfeI GAWTC 5 cut(s) 194, 869, 936, 1127, 1186
PflMI CCANNNNNTGG 1 cut(s) 524
PleI GAGTC 2 cut(s) 760, 1009
PpsI GAGTC 2 cut(s) 760, 1009
Ppu21I YACGTR 1 cut(s) 1013
PpuMI RGGWCCY 1 cut(s) 83
PsiI TTATAA 1 cut(s) 1193
Psp5II RGGWCCY 1 cut(s) 83
Psp6I CCWGG 1 cut(s) 1280
PspEI GGTNACC 1 cut(s) 7
PspGI CCWGG 1 cut(s) 1280
PspN4I GGNNCC 5 cut(s) 21, 84, 172, 516, 673
PspOMI GGGCCC 1 cut(s) 19
PspPI GGNCC 5 cut(s) 19, 20, 83, 449, 527
PspPPI RGGWCCY 1 cut(s) 83
PstI CTGCAG 1 cut(s) 1144
PstNI CAGNNNCTG 2 cut(s) 357, 1004
PsuI RGATCY 1 cut(s) 514
PvuII CAGCTG 1 cut(s) 892
RsaI GTAC 6 cut(s) 59, 355, 361, 789, 966, 1100
RsaNI GTAC 6 cut(s) 58, 354, 360, 788, 965, 1099
RseI CAYNNNNRTG 1 cut(s) 882
SaqAI TTAA 2 cut(s) 297, 614
Sau3AI GATC 4 cut(s) 472, 514, 832, 1074
Sau96I GGNCC 5 cut(s) 19, 20, 83, 449, 527
SchI GAGTC 2 cut(s) 761, 1010
ScrFI CCNGG 2 cut(s) 1235, 1282
SduI GDGCHC 2 cut(s) 23, 662
SfaNI GCATC 2 cut(s) 553, 628
SfcI CTRYAG 1 cut(s) 1140
SinI GGWCC 3 cut(s) 83, 449, 527
SmiMI CAYNNNNRTG 1 cut(s) 882
SmlI CTYRAG 1 cut(s) 844
SmoI CTYRAG 1 cut(s) 844
SphI GCATGC 1 cut(s) 1224
Sse9I AATT 5 cut(s) 338, 744, 948, 1047, 1194
SsiI CCGC 2 cut(s) 5, 1113
SspI AATATT 1 cut(s) 1260
SspMI CTAG 4 cut(s) 167, 219, 578, 1094
StyD4I CCNGG 2 cut(s) 1233, 1280
StyI CCWWGG 3 cut(s) 274, 675, 941
TaaI ACNGT 6 cut(s) 264, 353, 553, 915, 988, 1255
TaiI ACGT 3 cut(s) 742, 829, 1015
TaqI TCGA 2 cut(s) 336, 742
TasI AATT 5 cut(s) 338, 744, 948, 1047, 1194
TatI WGTACW 2 cut(s) 57, 359
TfiI GAWTC 5 cut(s) 194, 869, 936, 1127, 1186
Tru1I TTAA 2 cut(s) 297, 614
Tru9I TTAA 2 cut(s) 297, 614
TscAI CASTG 6 cut(s) 312, 692, 870, 1126, 1149, 1177
TseFI GTSAC 3 cut(s) 231, 775, 1147
Tsp45I GTSAC 3 cut(s) 231, 775, 1147
TspDTI ATGAA 6 cut(s) 62, 283, 297, 452, 908, 1119
TspGWI ACGGA 2 cut(s) 77, 1198
TspRI CASTG 6 cut(s) 312, 692, 870, 1126, 1149, 1177
Van91I CCANNNNNTGG 1 cut(s) 524
VpaK11BI GGWCC 3 cut(s) 83, 449, 527
XagI CCTNNNNNAGG 1 cut(s) 243
XapI RAATTY 2 cut(s) 338, 744
XbaI TCTAGA 1 cut(s) 1093
XceI RCATGY 3 cut(s) 631, 903, 1224
XcmI CCANNNNNNNNNTGG 1 cut(s) 673
XmnI GAANNNNTTC 1 cut(s) 1048
XspI CTAG 4 cut(s) 167, 219, 578, 1094
ZraI GACGTC 1 cut(s) 740
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.