FvH4_7g32880

START domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0003829
Physical Location & Seq
Reverse (-)
23555733 .. 23559132
3400 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g32880.t1

Sequence Viewer

Length: 1320 bp
ATGGGTCTGATTGGTGCGCTGCATGAGATTTTGGAGATGGTGAAAATAGAGTACACCGACTTTGTGATGCTTCTGGGTCCTTTGTGGACGGCTTTTCTTATTGGTATTGTGATTGGTTGGGCGTGGAAGCCAAAGTGGGCAACATTTGGTCAGGAAAAACTGAACTCCTCTGTTTCTAAAGCCTTGGATTTGTCATCCATGCCTACTTCTCCGGCTAGGTCTGTGATGTCACCACCACTAAAAGGGTTCTCATCAGCACCGTGCTTGTTCCAGGGCTATGAAGCTTGGATTATTAACAATGCAGTTCATAATGCTGTGCAGAAGAGGCTTTCTCCCTCACCTATCGAATTTGATGACCAGAGTACATCGCAGATGAATGAGAAGAAGTCTGATGTGGTGGCTCAGGAGGATGTAGAACATCTATGTCAGCTTGTTGATATGAAAGATGGAGGTCCGAGTTGGATACATATGATGGATCGTTCGACTCCAACTATGACTTGTCAAGTTTGGCGTAGGGATCCCAAGATTGGTCCTCCTCAATATCGTAGCAGGACTGTGTATGAGGATGTAACACCGGAGCTAGTGAGGGACTTTTTCTGGGATGATGAGTTCCGGTTAAAGTGGGATGACATGCTTAGTGAGGCCACGACCTTAGATGAGTGCCCAACCACAGGAACAATGGTTGTTCAGTGGATAAAAAAGTTCCCTTTCTTCTGTAAAGACAGAGAATACATAATAGGACGGCGAATTTGGGAGTCAGGAAGGTTGTATTATTGTGTGACAAAGGGAGTACCCTATCCTTCTATTCCAAGGCATGACAAACTAAGACGTGTGGATAACTACTACTCAAGTTGGTGCATTCAAGCAGCTGAATCAAGACACGACAATGGCCAGCTCAGTGCATGTGAAGTTCAACTGTTTCATCAAGAGGATATGGGGATCCCATGGGAAATCGCAAAACTTGCTGTACGAAAAGGAATGTGGGATACTGTAAAGAACATAGAGTCTGGTTTACGTGCTTACCAGAAACAAAGAGCGTTAGGGGGGATAATTTCTCGGCCTGCCTTTATGGCGCAAATCAACACCAAAATAAGTCTAGAGTATCTGAAAGCTTCCGCTGGCACCGAGGTTTCATCACAAACTGCAGTGGTGACTGCATCTGATGACAAGCCTTTCGGTGGGAACATACGAAATCTCATAATAATTGGTGGGAGTATTCTTCTTGCATGCACTCTTGACCGGGGGTATCTGGTAAAGACAATAATATCCGGTGTGGCCAATAAGTTCGGAAATGTGGGGAAGAAATCAAAACCAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
START

Protein Analysis

440

Amino Acids

49.6

Weight (kDa)

8.43

Isoelectric Point (pI)

46.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
START PF01852 179 - 332 2.1e-07 START domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1121
AciI CCGC 1 cut(s) 1116
AclWI GGATC 5 cut(s) 483, 512, 525, 934, 947
AcoI YGGCCR 2 cut(s) 889, 1275
AcsI RAATTY 2 cut(s) 347, 747
AfaI GTAC 4 cut(s) 53, 364, 792, 969
AfiI CCNNNNNNNGG 4 cut(s) 242, 527, 671, 1178
AflIII ACRYGT 1 cut(s) 829
AgsI TTSAA 2 cut(s) 863, 914
AjiI CACGTC 1 cut(s) 830
AjnI CCWGG 1 cut(s) 270
AloI GAACNNNNNNTCC 2 cut(s) 593, 625
AluBI AGCT 6 cut(s) 284, 430, 580, 869, 895, 1112
AluI AGCT 6 cut(s) 284, 430, 580, 869, 895, 1112
AlwI GGATC 5 cut(s) 483, 512, 525, 934, 947
AoxI GGCC 4 cut(s) 642, 889, 1058, 1275
ApeKI GCWGC 2 cut(s) 19, 866
ApoI RAATTY 2 cut(s) 347, 747
ArsI GACNNNNNNTTYG 4 cut(s) 732, 764, 1157, 1189
AspLEI GCGC 2 cut(s) 19, 1075
AspS9I GGNCC 3 cut(s) 77, 452, 530
AsuC2I CCSGG 1 cut(s) 1241
AsuHPI GGTGA 4 cut(s) 52, 222, 330, 1162
AvaII GGWCC 3 cut(s) 77, 452, 530
BaeGI GKGCMC 1 cut(s) 665
BalI TGGCCA 2 cut(s) 891, 1277
BamHI GGATCC 2 cut(s) 517, 939
BanI GGYRCC 1 cut(s) 1121
BbvI GCAGC 2 cut(s) 6, 878
BccI CCATC 3 cut(s) 31, 440, 466
BceAI ACGGC 2 cut(s) 105, 758
BciT130I CCWGG 1 cut(s) 272
BciVI GTATCC 2 cut(s) 456, 979
BcnI CCSGG 1 cut(s) 1241
BfaI CTAG 3 cut(s) 216, 581, 1097
BfmI CTRYAG 1 cut(s) 1143
BfuI GTATCC 2 cut(s) 456, 979
BglI GCCNNNNNGGC 1 cut(s) 1070
BisI GCNGC 2 cut(s) 20, 867
BlsI GCNGC 2 cut(s) 21, 868
Bme1390I CCNGG 2 cut(s) 272, 1241
Bme18I GGWCC 3 cut(s) 77, 452, 530
BmgBI CACGTC 1 cut(s) 830
BmgT120I GGNCC 3 cut(s) 77, 452, 530
BmiI GGNNCC 4 cut(s) 78, 519, 941, 1123
BmrFI CCNGG 2 cut(s) 272, 1241
BmsI GCATC 2 cut(s) 57, 1166
BplI GAGNNNNNCTC 2 cut(s) 316, 348
Bpu10I CCTNAGC 1 cut(s) 402
BpuEI CTTGAG 1 cut(s) 832
BpuMI CCSGG 1 cut(s) 1241
BsaAI YACGTR 1 cut(s) 1016
BsaJI CCNNGG 6 cut(s) 183, 271, 809, 944, 1125, 1240
BsaWI WCCGGW 3 cut(s) 574, 612, 1268
Bsc4I CCNNNNNNNGG 4 cut(s) 242, 527, 671, 1178
BseBI CCWGG 1 cut(s) 272
BseDI CCNNGG 6 cut(s) 183, 271, 809, 944, 1125, 1240
BseGI GGATG 5 cut(s) 194, 415, 571, 607, 631
BseLI CCNNNNNNNGG 4 cut(s) 242, 527, 671, 1178
BseMII CTCAG 2 cut(s) 416, 910
BseRI GAGGAG 2 cut(s) 157, 525
BseSI GKGCMC 1 cut(s) 665
BseXI GCAGC 2 cut(s) 6, 878
BsgI GTGCAG 1 cut(s) 338
BshFI GGCC 4 cut(s) 644, 891, 1060, 1277
BshNI GGYRCC 1 cut(s) 1121
BsiSI CCGG 5 cut(s) 212, 575, 613, 1240, 1269
BslFI GGGAC 1 cut(s) 602
BslI CCNNNNNNNGG 4 cut(s) 242, 527, 671, 1178
BsmFI GGGAC 1 cut(s) 602
BsmI GAATGC 1 cut(s) 858
BsnI GGCC 4 cut(s) 644, 891, 1060, 1277
Bsp1286I GDGCHC 1 cut(s) 665
Bsp143I GATC 3 cut(s) 475, 517, 939
Bsp19I CCATGG 1 cut(s) 944
BspACI CCGC 1 cut(s) 1116
BspANI GGCC 4 cut(s) 644, 891, 1060, 1277
BspCNI CTCAG 2 cut(s) 415, 909
BspLI GGNNCC 4 cut(s) 78, 519, 941, 1123
BspMAI CTGCAG 1 cut(s) 1147
BspPI GGATC 5 cut(s) 483, 512, 525, 934, 947
BspT107I GGYRCC 1 cut(s) 1121
BssECI CCNNGG 6 cut(s) 183, 271, 809, 944, 1125, 1240
BssMI GATC 3 cut(s) 475, 517, 939
BssT1I CCWWGG 3 cut(s) 183, 809, 944
Bst2UI CCWGG 1 cut(s) 272
Bst4CI ACNGT 4 cut(s) 261, 556, 918, 991
Bst6I CTCTTC 1 cut(s) 317
BstAPI GCANNNNNTGC 1 cut(s) 962
BstBAI YACGTR 1 cut(s) 1016
BstC8I GCNNGC 4 cut(s) 893, 1062, 1120, 1228
BstDEI CTNAG 5 cut(s) 402, 635, 652, 824, 896
BstDSI CCRYGG 1 cut(s) 944
BstF5I GGATG 5 cut(s) 194, 415, 571, 607, 631
BstHHI GCGC 2 cut(s) 19, 1075
BstKTI GATC 3 cut(s) 478, 520, 942
BstMBI GATC 3 cut(s) 475, 517, 939
BstMWI GCNNNNNNNGC 3 cut(s) 325, 962, 1070
BstNI CCWGG 1 cut(s) 272
BstNSI RCATGY 3 cut(s) 634, 906, 1230
BstSCI CCNGG 2 cut(s) 270, 1239
BstSFI CTRYAG 1 cut(s) 1143
BstSLI GKGCMC 1 cut(s) 665
BstV1I GCAGC 2 cut(s) 6, 878
BstX2I RGATCY 2 cut(s) 517, 939
BstYI RGATCY 2 cut(s) 517, 939
BsuI GTATCC 2 cut(s) 456, 979
BsuRI GGCC 4 cut(s) 644, 891, 1060, 1277
BtgI CCRYGG 1 cut(s) 944
BtgZI GCGATG 1 cut(s) 351
BtrI CACGTC 1 cut(s) 830
BtsCI GGATG 5 cut(s) 194, 415, 571, 607, 631
BtsI GCAGTG 1 cut(s) 1152
BtsIMutI CAGTG 3 cut(s) 695, 904, 1152
Cac8I GCNNGC 4 cut(s) 893, 1062, 1120, 1228
CfoI GCGC 2 cut(s) 19, 1075
Cfr13I GGNCC 3 cut(s) 77, 452, 530
Csp6I GTAC 4 cut(s) 52, 363, 791, 968
CviAII CATG 7 cut(s) 23, 199, 631, 815, 903, 945, 1227
CviQI GTAC 4 cut(s) 52, 363, 791, 968
DdeI CTNAG 5 cut(s) 402, 635, 652, 824, 896
DpnI GATC 3 cut(s) 477, 519, 941
DpnII GATC 3 cut(s) 475, 517, 939
EaeI YGGCCR 2 cut(s) 889, 1275
Eam1104I CTCTTC 1 cut(s) 317
EarI CTCTTC 1 cut(s) 317
Eco130I CCWWGG 3 cut(s) 183, 809, 944
Eco47I GGWCC 3 cut(s) 77, 452, 530
EcoO109I RGGNCCY 1 cut(s) 77
EcoRII CCWGG 1 cut(s) 270
EcoT14I CCWWGG 3 cut(s) 183, 809, 944
ErhI CCWWGG 3 cut(s) 183, 809, 944
FaeI CATG 7 cut(s) 26, 202, 634, 818, 906, 948, 1230
FaqI GGGAC 1 cut(s) 602
FatI CATG 7 cut(s) 22, 198, 630, 814, 902, 944, 1226
FauNDI CATATG 1 cut(s) 468
Fnu4HI GCNGC 2 cut(s) 20, 867
FokI GGATG 5 cut(s) 181, 422, 578, 614, 638
Fsp4HI GCNGC 2 cut(s) 20, 867
FspBI CTAG 3 cut(s) 216, 581, 1097
GlaI GCGC 2 cut(s) 18, 1074
GluI GCNGC 2 cut(s) 20, 867
HaeIII GGCC 4 cut(s) 644, 891, 1060, 1277
HapII CCGG 5 cut(s) 212, 575, 613, 1240, 1269
HhaI GCGC 2 cut(s) 19, 1075
Hin1II CATG 7 cut(s) 26, 202, 634, 818, 906, 948, 1230
Hin6I GCGC 2 cut(s) 17, 1073
HinP1I GCGC 2 cut(s) 17, 1073
HindIII AAGCTT 2 cut(s) 282, 1110
HinfI GANTC 4 cut(s) 484, 755, 872, 1004
HpaII CCGG 5 cut(s) 212, 575, 613, 1240, 1269
HphI GGTGA 4 cut(s) 52, 222, 330, 1162
Hpy166II GTNNAC 3 cut(s) 54, 87, 1013
Hpy188I TCNGA 6 cut(s) 9, 391, 456, 1107, 1162, 1289
Hpy188III TCNNGA 7 cut(s) 152, 404, 759, 876, 926, 1097, 1235
Hpy8I GTNNAC 3 cut(s) 54, 87, 1013
HpyAV CCTTC 2 cut(s) 756, 810
HpyCH4III ACNGT 4 cut(s) 261, 556, 918, 991
HpyCH4IV ACGT 2 cut(s) 829, 1015
HpyCH4V TGCA 9 cut(s) 22, 302, 319, 858, 902, 1145, 1157, 1226, 1230
HpyF10VI GCNNNNNNNGC 3 cut(s) 325, 962, 1070
HpyF3I CTNAG 5 cut(s) 402, 635, 652, 824, 896
HpySE526I ACGT 2 cut(s) 829, 1015
Hsp92II CATG 7 cut(s) 26, 202, 634, 818, 906, 948, 1230
HspAI GCGC 2 cut(s) 17, 1073
Kzo9I GATC 3 cut(s) 475, 517, 939
LmnI GCTCC 1 cut(s) 577
Lsp1109I GCAGC 2 cut(s) 6, 878
LweI GCATC 2 cut(s) 57, 1166
MaeI CTAG 3 cut(s) 216, 581, 1097
MaeII ACGT 2 cut(s) 829, 1015
MaeIII GTNAC 4 cut(s) 228, 568, 778, 1150
MalI GATC 3 cut(s) 477, 519, 941
MboI GATC 3 cut(s) 475, 517, 939
MboII GAAGA 5 cut(s) 334, 394, 703, 1211, 1312
MflI RGATCY 2 cut(s) 517, 939
MhlI GDGCHC 1 cut(s) 665
MlsI TGGCCA 2 cut(s) 891, 1277
MluCI AATT 4 cut(s) 347, 747, 1050, 1203
MluNI TGGCCA 2 cut(s) 891, 1277
MlyI GAGTC 3 cut(s) 478, 764, 1013
MmeI TCCRAC 2 cut(s) 440, 512
Mox20I TGGCCA 2 cut(s) 891, 1277
MscI TGGCCA 2 cut(s) 891, 1277
MseI TTAA 2 cut(s) 294, 617
MslI CAYNNNNRTG 1 cut(s) 885
Msp20I TGGCCA 2 cut(s) 891, 1277
MspA1I CMGCKG 2 cut(s) 869, 1118
MspI CCGG 5 cut(s) 212, 575, 613, 1240, 1269
MspR9I CCNGG 2 cut(s) 272, 1241
Mva1269I GAATGC 1 cut(s) 858
MvaI CCWGG 1 cut(s) 272
MwoI GCNNNNNNNGC 3 cut(s) 325, 962, 1070
NciI CCSGG 1 cut(s) 1241
NcoI CCATGG 1 cut(s) 944
NdeI CATATG 1 cut(s) 468
NdeII GATC 3 cut(s) 475, 517, 939
NlaIII CATG 7 cut(s) 26, 202, 634, 818, 906, 948, 1230
NlaIV GGNNCC 4 cut(s) 78, 519, 941, 1123
NmeAIII GCCGAG 1 cut(s) 1036
NmuCI GTSAC 3 cut(s) 228, 778, 1150
NspI RCATGY 3 cut(s) 634, 906, 1230
PaeI GCATGC 1 cut(s) 1230
PctI GAATGC 1 cut(s) 858
PfeI GAWTC 1 cut(s) 872
PkrI GCNGC 2 cut(s) 21, 868
PleI GAGTC 3 cut(s) 478, 763, 1012
PpsI GAGTC 3 cut(s) 478, 763, 1012
Ppu21I YACGTR 1 cut(s) 1016
PpuMI RGGWCCY 1 cut(s) 77
Psp5II RGGWCCY 1 cut(s) 77
Psp6I CCWGG 1 cut(s) 270
PspGI CCWGG 1 cut(s) 270
PspN4I GGNNCC 4 cut(s) 78, 519, 941, 1123
PspPI GGNCC 3 cut(s) 77, 452, 530
PspPPI RGGWCCY 1 cut(s) 77
PstI CTGCAG 1 cut(s) 1147
PsuI RGATCY 2 cut(s) 517, 939
PvuII CAGCTG 1 cut(s) 869
RsaI GTAC 4 cut(s) 53, 364, 792, 969
RsaNI GTAC 4 cut(s) 52, 363, 791, 968
RseI CAYNNNNRTG 1 cut(s) 885
SaqAI TTAA 2 cut(s) 294, 617
SatI GCNGC 2 cut(s) 20, 867
Sau3AI GATC 3 cut(s) 475, 517, 939
Sau96I GGNCC 3 cut(s) 77, 452, 530
SchI GAGTC 3 cut(s) 478, 764, 1013
ScrFI CCNGG 2 cut(s) 272, 1241
SduI GDGCHC 1 cut(s) 665
SfaNI GCATC 2 cut(s) 57, 1166
SfcI CTRYAG 1 cut(s) 1143
SinI GGWCC 3 cut(s) 77, 452, 530
SmiMI CAYNNNNRTG 1 cut(s) 885
SmlI CTYRAG 1 cut(s) 847
SmoI CTYRAG 1 cut(s) 847
SphI GCATGC 1 cut(s) 1230
Sse9I AATT 4 cut(s) 347, 747, 1050, 1203
SsiI CCGC 1 cut(s) 1116
SspMI CTAG 3 cut(s) 216, 581, 1097
StyD4I CCNGG 2 cut(s) 270, 1239
StyI CCWWGG 3 cut(s) 183, 809, 944
TaaI ACNGT 4 cut(s) 261, 556, 918, 991
TaiI ACGT 2 cut(s) 832, 1018
TaqI TCGA 2 cut(s) 345, 482
TasI AATT 4 cut(s) 347, 747, 1050, 1203
TatI WGTACW 2 cut(s) 51, 362
TfiI GAWTC 1 cut(s) 872
Tru1I TTAA 2 cut(s) 294, 617
Tru9I TTAA 2 cut(s) 294, 617
TscAI CASTG 3 cut(s) 695, 904, 1152
TseFI GTSAC 3 cut(s) 228, 778, 1150
TseI GCWGC 2 cut(s) 19, 866
Tsp45I GTSAC 3 cut(s) 228, 778, 1150
TspDTI ATGAA 6 cut(s) 294, 296, 389, 455, 911, 1122
TspRI CASTG 3 cut(s) 695, 904, 1152
VpaK11BI GGWCC 3 cut(s) 77, 452, 530
XapI RAATTY 2 cut(s) 347, 747
XbaI TCTAGA 1 cut(s) 1096
XceI RCATGY 3 cut(s) 634, 906, 1230
XcmI CCANNNNNNNNNTGG 1 cut(s) 676
XspI CTAG 3 cut(s) 216, 581, 1097
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.