Rh7CG088300

START domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0003829
Physical Location & Seq
Reverse (-)
6670448 .. 6676164
5717 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG088300.1

Sequence Viewer

Length: 975 bp
ATGAATCAATGTTTTGTCTCTGGCAGTTCCTCACAGATTGAACAAGAAGAATCCTGTAATGTCTCCAATGAGGATCTACGCCACATATGTCAGCTTGTGGAGGAGAAAGATGGCGGTCCTGCTTGGATTCATATGATGGATCGGTCAACCCCAACTATGGGATATCAAGCTTGGCGTAGGGATCCTCAGACTGGACCTCCACAATACCGAAGTAGAACTGTATTCGAAGATGCTTCACCTGAGACAGTGAGGGATTTCTTTTGGGATGATGAATTTCGTGCAAAGTGGGATGATATGCTTATTCATGCTTCTACTTTGGAAGAATGCCCGACTACTGGAACCATGCTTGTGCACTGGGTCCGCAAGTTTCCATTCTTTTGTAGCGACAGAGATTACACGATAGGCCGTCGCATTTGGGAATCGGGGCGATCATACTACTGTGTAACTAAGGGAGTTCCTTCCTCTGCTGTGCCAAGGCGTGACAAACCTAGACGTGTTGATCTGTACTATTCAAGTTGGTGCATACGTGCAGTTGAATCAAAAAGAGGGGATGGCCAGCTTACTTCATGTGAGGTTTTACTGTTTCACCATGAAGATATGGGTATTCCATGGGAGATTGCGAAACTTGGTGTTCGTCAAGGTATGTGGGGAGCTGTTAAAAAGATTGAACCAGGCCTACGTTTATATCAGAAGGAAAGAGCCGCTGAAGCACCACTTTCGCGGTGTGCTAAAATGGCTCAGATCAACACGAAAGTCAGTGCTGATTACTTGAGATCGGTAGAAAACAGCACTAAGGATTCCATGGAGGTTGAAAATTCAGATTCAGCGGAGAAACCAGGAAGGAGGAATATACCTAAAGCTCTAGTTGTCGGAGGGGCAATAGTTCTTGCTTGCAGTCTTGATCGGGGACTCTTGACTAAGGCAGTTATATTTGGAGTAGCTCGAAGATTTGCCAAGATCGGGAGGAGGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
START

Protein Analysis

324

Amino Acids

36.93

Weight (kDa)

8.63

Isoelectric Point (pI)

58.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
START PF01852 41 - 196 1.2e-08 START domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 721
AciI CCGC 5 cut(s) 114, 361, 702, 721, 827
AclWI GGATC 4 cut(s) 81, 147, 176, 189
AcoI YGGCCR 1 cut(s) 553
AcsI RAATTY 2 cut(s) 272, 814
AcuI CTGAAG 1 cut(s) 726
AfaI GTAC 1 cut(s) 506
AfiI CCNNNNNNNGG 5 cut(s) 157, 158, 191, 335, 960
AflIII ACRYGT 1 cut(s) 493
AgsI TTSAA 5 cut(s) 41, 513, 536, 668, 812
AjiI CACGTC 1 cut(s) 494
AjnI CCWGG 2 cut(s) 670, 835
AluBI AGCT 6 cut(s) 94, 170, 559, 653, 860, 941
AluI AGCT 6 cut(s) 94, 170, 559, 653, 860, 941
Alw21I GWGCWC 1 cut(s) 354
Alw26I GTCTC 3 cut(s) 22, 67, 236
Alw44I GTGCAC 1 cut(s) 350
AlwI GGATC 4 cut(s) 81, 147, 176, 189
AoxI GGCC 3 cut(s) 403, 553, 673
ApaLI GTGCAC 1 cut(s) 350
ApoI RAATTY 2 cut(s) 272, 814
AspS9I GGNCC 3 cut(s) 116, 194, 358
AsuHPI GGTGA 2 cut(s) 228, 578
AsuII TTCGAA 1 cut(s) 225
AvaII GGWCC 3 cut(s) 116, 194, 358
BaeGI GKGCMC 1 cut(s) 354
BalI TGGCCA 1 cut(s) 555
BamHI GGATCC 1 cut(s) 181
Bbv12I GWGCWC 1 cut(s) 354
BccI CCATC 3 cut(s) 104, 130, 545
BceAI ACGGC 1 cut(s) 390
BcgI CGANNNNNNTGC 2 cut(s) 699, 733
BciT130I CCWGG 2 cut(s) 672, 837
BcoDI GTCTC 3 cut(s) 22, 67, 236
BfaI CTAG 2 cut(s) 489, 863
BisI GCNGC 1 cut(s) 702
BlsI GCNGC 1 cut(s) 703
Bme1390I CCNGG 2 cut(s) 672, 837
Bme18I GGWCC 3 cut(s) 116, 194, 358
BmgBI CACGTC 1 cut(s) 494
BmgT120I GGNCC 3 cut(s) 116, 194, 358
BmiI GGNNCC 3 cut(s) 183, 340, 359
BmrFI CCNGG 2 cut(s) 672, 837
BmrI ACTGGG 1 cut(s) 364
BmsI GCATC 1 cut(s) 220
BmuI ACTGGG 1 cut(s) 364
Bpu14I TTCGAA 1 cut(s) 225
BpuEI CTTGAG 1 cut(s) 790
BsaAI YACGTR 1 cut(s) 527
BsaJI CCNNGG 3 cut(s) 473, 608, 801
BsaXI ACNNNNNCTCC 2 cut(s) 181, 211
Bsc4I CCNNNNNNNGG 5 cut(s) 157, 158, 191, 335, 960
Bse1I ACTGG 3 cut(s) 196, 340, 359
BseBI CCWGG 2 cut(s) 672, 837
BseDI CCNNGG 3 cut(s) 473, 608, 801
BseGI GGATG 3 cut(s) 271, 295, 556
BseLI CCNNNNNNNGG 5 cut(s) 157, 158, 191, 335, 960
BseMII CTCAG 3 cut(s) 200, 231, 752
BseNI ACTGG 3 cut(s) 196, 340, 359
BseRI GAGGAG 1 cut(s) 116
BseSI GKGCMC 1 cut(s) 354
BsgI GTGCAG 1 cut(s) 549
Bsh1236I CGCG 1 cut(s) 721
BshFI GGCC 3 cut(s) 405, 555, 675
BsiHKAI GWGCWC 1 cut(s) 354
BslFI GGGAC 1 cut(s) 921
BslI CCNNNNNNNGG 5 cut(s) 157, 158, 191, 335, 960
BsmAI GTCTC 3 cut(s) 22, 67, 236
BsmFI GGGAC 1 cut(s) 921
BsmI GAATGC 1 cut(s) 329
BsnI GGCC 3 cut(s) 405, 555, 675
Bsp119I TTCGAA 1 cut(s) 225
Bsp1286I GDGCHC 1 cut(s) 354
Bsp143I GATC 9 cut(s) 73, 139, 181, 428, 499, 741, 773, 901, 957
Bsp19I CCATGG 2 cut(s) 608, 801
BspACI CCGC 5 cut(s) 114, 361, 702, 721, 827
BspANI GGCC 3 cut(s) 405, 555, 675
BspCNI CTCAG 3 cut(s) 199, 232, 751
BspFNI CGCG 1 cut(s) 721
BspLI GGNNCC 3 cut(s) 183, 340, 359
BspPI GGATC 4 cut(s) 81, 147, 176, 189
BspT104I TTCGAA 1 cut(s) 225
BsrI ACTGG 3 cut(s) 196, 340, 359
BssECI CCNNGG 3 cut(s) 473, 608, 801
BssMI GATC 9 cut(s) 73, 139, 181, 428, 499, 741, 773, 901, 957
BssT1I CCWWGG 3 cut(s) 473, 608, 801
Bst2UI CCWGG 2 cut(s) 672, 837
Bst4CI ACNGT 4 cut(s) 220, 247, 440, 582
BstBAI YACGTR 1 cut(s) 527
BstBI TTCGAA 1 cut(s) 225
BstC8I GCNNGC 2 cut(s) 557, 892
BstDEI CTNAG 6 cut(s) 186, 240, 447, 738, 792, 918
BstDSI CCRYGG 2 cut(s) 608, 801
BstF5I GGATG 3 cut(s) 271, 295, 556
BstFNI CGCG 1 cut(s) 721
BstKTI GATC 9 cut(s) 76, 142, 184, 431, 502, 744, 776, 904, 960
BstMAI GTCTC 3 cut(s) 22, 67, 236
BstMBI GATC 9 cut(s) 73, 139, 181, 428, 499, 741, 773, 901, 957
BstMWI GCNNNNNNNGC 2 cut(s) 707, 734
BstNI CCWGG 2 cut(s) 672, 837
BstSCI CCNGG 2 cut(s) 670, 835
BstSLI GKGCMC 1 cut(s) 354
BstUI CGCG 1 cut(s) 721
BstX2I RGATCY 2 cut(s) 73, 181
BstYI RGATCY 2 cut(s) 73, 181
BsuRI GGCC 3 cut(s) 405, 555, 675
BtgI CCRYGG 2 cut(s) 608, 801
BtrI CACGTC 1 cut(s) 494
BtsCI GGATG 3 cut(s) 271, 295, 556
BtsIMutI CAGTG 3 cut(s) 252, 352, 763
Cac8I GCNNGC 2 cut(s) 557, 892
Cfr13I GGNCC 3 cut(s) 116, 194, 358
Csp6I GTAC 1 cut(s) 505
CspCI CAANNNNNGTGG 2 cut(s) 626, 661
CviAII CATG 6 cut(s) 305, 343, 567, 590, 609, 802
CviQI GTAC 1 cut(s) 505
DdeI CTNAG 6 cut(s) 186, 240, 447, 738, 792, 918
DpnI GATC 9 cut(s) 75, 141, 183, 430, 501, 743, 775, 903, 959
DpnII GATC 9 cut(s) 73, 139, 181, 428, 499, 741, 773, 901, 957
EaeI YGGCCR 1 cut(s) 553
Eco130I CCWWGG 3 cut(s) 473, 608, 801
Eco147I AGGCCT 1 cut(s) 675
Eco32I GATATC 1 cut(s) 164
Eco47I GGWCC 3 cut(s) 116, 194, 358
Eco57I CTGAAG 1 cut(s) 726
EcoRII CCWGG 2 cut(s) 670, 835
EcoRV GATATC 1 cut(s) 164
EcoT14I CCWWGG 3 cut(s) 473, 608, 801
ErhI CCWWGG 3 cut(s) 473, 608, 801
FaeI CATG 6 cut(s) 308, 346, 570, 593, 612, 805
FalI AAGNNNNNCTT 2 cut(s) 699, 731
FaqI GGGAC 1 cut(s) 921
FatI CATG 6 cut(s) 304, 342, 566, 589, 608, 801
FauNDI CATATG 2 cut(s) 86, 132
Fnu4HI GCNGC 1 cut(s) 702
FokI GGATG 3 cut(s) 278, 302, 563
Fsp4HI GCNGC 1 cut(s) 702
FspBI CTAG 2 cut(s) 489, 863
GluI GCNGC 1 cut(s) 702
HaeIII GGCC 3 cut(s) 405, 555, 675
Hin1II CATG 6 cut(s) 308, 346, 570, 593, 612, 805
HincII GTYRAC 1 cut(s) 147
HindII GTYRAC 1 cut(s) 147
HindIII AAGCTT 1 cut(s) 168
HinfI GANTC 8 cut(s) 4, 50, 127, 419, 536, 797, 821, 909
HphI GGTGA 2 cut(s) 228, 578
Hpy166II GTNNAC 2 cut(s) 147, 352
Hpy188I TCNGA 5 cut(s) 189, 690, 741, 820, 872
Hpy188III TCNNGA 3 cut(s) 899, 913, 961
Hpy8I GTNNAC 2 cut(s) 147, 352
Hpy99I CGWCG 1 cut(s) 411
HpyAV CCTTC 3 cut(s) 468, 685, 834
HpyCH4III ACNGT 4 cut(s) 220, 247, 440, 582
HpyCH4IV ACGT 3 cut(s) 493, 526, 679
HpyCH4V TGCA 5 cut(s) 281, 352, 522, 530, 894
HpyF10VI GCNNNNNNNGC 2 cut(s) 707, 734
HpyF3I CTNAG 6 cut(s) 186, 240, 447, 738, 792, 918
HpySE526I ACGT 3 cut(s) 493, 526, 679
Hsp92II CATG 6 cut(s) 308, 346, 570, 593, 612, 805
Kzo9I GATC 9 cut(s) 73, 139, 181, 428, 499, 741, 773, 901, 957
LmnI GCTCC 1 cut(s) 650
LweI GCATC 1 cut(s) 220
MaeI CTAG 2 cut(s) 489, 863
MaeII ACGT 3 cut(s) 493, 526, 679
MaeIII GTNAC 2 cut(s) 442, 479
MalI GATC 9 cut(s) 75, 141, 183, 430, 501, 743, 775, 903, 959
MboI GATC 9 cut(s) 73, 139, 181, 428, 499, 741, 773, 901, 957
MboII GAAGA 5 cut(s) 59, 239, 332, 605, 957
MflI RGATCY 2 cut(s) 73, 181
MhlI GDGCHC 1 cut(s) 354
MlsI TGGCCA 1 cut(s) 555
MluCI AATT 2 cut(s) 272, 814
MluNI TGGCCA 1 cut(s) 555
MlyI GAGTC 1 cut(s) 903
MmeI TCCRAC 1 cut(s) 850
Mox20I TGGCCA 1 cut(s) 555
MscI TGGCCA 1 cut(s) 555
MseI TTAA 1 cut(s) 657
MslI CAYNNNNRTG 1 cut(s) 347
Msp20I TGGCCA 1 cut(s) 555
MspA1I CMGCKG 2 cut(s) 704, 827
MspR9I CCNGG 2 cut(s) 672, 837
Mva1269I GAATGC 1 cut(s) 329
MvaI CCWGG 2 cut(s) 672, 837
MvnI CGCG 1 cut(s) 721
MwoI GCNNNNNNNGC 2 cut(s) 707, 734
NcoI CCATGG 2 cut(s) 608, 801
NdeI CATATG 2 cut(s) 86, 132
NdeII GATC 9 cut(s) 73, 139, 181, 428, 499, 741, 773, 901, 957
NlaIII CATG 6 cut(s) 308, 346, 570, 593, 612, 805
NlaIV GGNNCC 3 cut(s) 183, 340, 359
NmuCI GTSAC 1 cut(s) 479
NspV TTCGAA 1 cut(s) 225
PceI AGGCCT 1 cut(s) 675
PctI GAATGC 1 cut(s) 329
PfeI GAWTC 7 cut(s) 4, 50, 127, 419, 536, 797, 821
PkrI GCNGC 1 cut(s) 703
PleI GAGTC 1 cut(s) 903
PpsI GAGTC 1 cut(s) 903
Ppu21I YACGTR 1 cut(s) 527
Psp6I CCWGG 2 cut(s) 670, 835
PspGI CCWGG 2 cut(s) 670, 835
PspN4I GGNNCC 3 cut(s) 183, 340, 359
PspPI GGNCC 3 cut(s) 116, 194, 358
PsrI GAACNNNNNNTAC 2 cut(s) 660, 692
PsuI RGATCY 2 cut(s) 73, 181
RsaI GTAC 1 cut(s) 506
RsaNI GTAC 1 cut(s) 505
RseI CAYNNNNRTG 1 cut(s) 347
SaqAI TTAA 1 cut(s) 657
SatI GCNGC 1 cut(s) 702
Sau3AI GATC 9 cut(s) 73, 139, 181, 428, 499, 741, 773, 901, 957
Sau96I GGNCC 3 cut(s) 116, 194, 358
SchI GAGTC 1 cut(s) 903
ScrFI CCNGG 2 cut(s) 672, 837
SduI GDGCHC 1 cut(s) 354
SfaNI GCATC 1 cut(s) 220
SfuI TTCGAA 1 cut(s) 225
SinI GGWCC 3 cut(s) 116, 194, 358
SmiMI CAYNNNNRTG 1 cut(s) 347
SmlI CTYRAG 1 cut(s) 769
SmoI CTYRAG 1 cut(s) 769
Sse9I AATT 2 cut(s) 272, 814
SseBI AGGCCT 1 cut(s) 675
SsiI CCGC 5 cut(s) 114, 361, 702, 721, 827
SspMI CTAG 2 cut(s) 489, 863
StuI AGGCCT 1 cut(s) 675
StyD4I CCNGG 2 cut(s) 670, 835
StyI CCWWGG 3 cut(s) 473, 608, 801
TaaI ACNGT 4 cut(s) 220, 247, 440, 582
TaiI ACGT 3 cut(s) 496, 529, 682
TaqI TCGA 2 cut(s) 225, 943
TaqII GACCGA 1 cut(s) 132
TasI AATT 2 cut(s) 272, 814
TatI WGTACW 1 cut(s) 504
TauI GCSGC 1 cut(s) 704
TfiI GAWTC 7 cut(s) 4, 50, 127, 419, 536, 797, 821
Tru1I TTAA 1 cut(s) 657
Tru9I TTAA 1 cut(s) 657
TscAI CASTG 3 cut(s) 252, 359, 763
TseFI GTSAC 1 cut(s) 479
Tsp45I GTSAC 1 cut(s) 479
TspDTI ATGAA 6 cut(s) 17, 119, 285, 293, 555, 606
TspRI CASTG 3 cut(s) 252, 359, 763
VneI GTGCAC 1 cut(s) 350
VpaK11BI GGWCC 3 cut(s) 116, 194, 358
XapI RAATTY 2 cut(s) 272, 814
XspI CTAG 2 cut(s) 489, 863
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.