Rroxscaffold_3G00267260

START domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0003829
Physical Location & Seq
Forward (+)
60353591 .. 60358851
5261 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00267260.1

Sequence Viewer

Length: 1290 bp
ATGGCTTTGGCCTCGGCTTTGCTGGAGATTCTGCGAAGACCCACGATTGTTGAAGTGCTGGGGGAGCTCGTGAAGTTCATAACCCCTCTTTGGATTGCTGTGATTGTCGGGGTTTTCGTTGGGTGGGCGTGGCGGCCCAAATGGGTGACCGTGGTTGGTAGAGAATTGTTGGATAGCTCCACACAAAAGGAGTCATCTTCCTCGTCTTCTTCATCTCCGCCCGCACCATCCACGTGTGGTATTTCGATTTCGAGTTTGAGTTCGTTGCTGCCAAATTGCATTCCTTGGGCTGCTGACGATAGAAATCAGAACCAGGCACCTACCACCAATAATGATTGCAGTTCCTCACAGATTGAACAAGAAGAATCCTGTAATGTCTCCAATGAGGATTTACGCCACATATGTCGGCTTGTGGAGGAGAAAGATGGCGGTCCTGCTTGGATTCATATGATGGATCGGTCAACCCCAACTATGGGATATCAAGCTTGGCGTAGAGATCCTCAGACTGGACCTCCACAATACCGAAGTAGAACTGTATTCGAAGATGCTTCACCGGAGACAGTGAGGGATTTCTTTTGGGATGATGAATTTCGTGCAAAGTGGGATGATATGCTTATTCATGCTTCTACTTTGGAAGAATGCCCGACTACTGGAACCATGCTAGTGCACTGGGTCCGCAAGTTTCCATTCTTTTGTAGCGACAGAGATTACACGATAGGCCGTCGCATTTGGGAATCGGGGCGATCATACTACTGTGTAACTAAGGGAGTTCCTTCCTCTGCTGTGCCAAGGCGTGACAAACCTAGACGTGTTGATCTGTACTATTCAAGTTGGTGCATTCGTGCAGTTGAATCAAAAAGAGGGGATGGCCAGCTTACTTCATGTGAGGTTTTACTGTTTCACCATGAAGATATGGGTATTCCATGGGAGATTGCAAAACTTGGAGTTCGTCAAGGTATGTGGGGAGCTGTTAAAAAGATTGAACCAGGCCTACGTTTATATCAGAAGGAAAGAGCTGCTAAAGCGCCACTTTCGCGGTGTGCTAAAATGGCTCAGATCAACACGAAAGTCAGTGCTGATTACTTGAGATCGGTAGAAAACAGCACTAAGGATTCCATGGAGGTTGAAAATTCAGATCCAGCGGAGAAACCAGCAAGGAGGAACATACCTAAAGCTCTAGTTGTCGGAGGGGCAATAGTTCTTGCTTGCAGTCTTGATCGGGGCCTCTTGACTAAGGCAGTTATATTTGGAGTAGCTCGAAGATTTGCCAAGATCGGGAGGAGGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
START

Protein Analysis

429

Amino Acids

48.25

Weight (kDa)

8.76

Isoelectric Point (pI)

57.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
START PF01852 146 - 301 2.1e-08 START domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 316
AccII CGCG 1 cut(s) 1036
AciI CCGC 7 cut(s) 133, 218, 222, 429, 676, 1036, 1142
AclWI GGATC 3 cut(s) 462, 491, 1130
AcoI YGGCCR 1 cut(s) 868
AcsI RAATTY 2 cut(s) 587, 1129
AcvI CACGTG 1 cut(s) 234
AfaI GTAC 1 cut(s) 821
AfiI CCNNNNNNNGG 6 cut(s) 90, 472, 473, 506, 650, 1275
AflIII ACRYGT 2 cut(s) 233, 808
AgsI TTSAA 6 cut(s) 53, 356, 828, 851, 983, 1127
AjiI CACGTC 1 cut(s) 809
AjnI CCWGG 2 cut(s) 312, 985
AluBI AGCT 8 cut(s) 67, 177, 485, 874, 968, 1016, 1175, 1256
AluI AGCT 8 cut(s) 67, 177, 485, 874, 968, 1016, 1175, 1256
Alw21I GWGCWC 2 cut(s) 69, 669
Alw26I GTCTC 2 cut(s) 382, 551
Alw44I GTGCAC 1 cut(s) 665
AlwI GGATC 3 cut(s) 462, 491, 1130
AoxI GGCC 6 cut(s) 9, 134, 718, 868, 988, 1222
ApaLI GTGCAC 1 cut(s) 665
ApeKI GCWGC 3 cut(s) 268, 290, 1016
ApoI RAATTY 2 cut(s) 587, 1129
AspLEI GCGC 1 cut(s) 1027
AspS9I GGNCC 5 cut(s) 135, 431, 509, 673, 1222
AsuHPI GGTGA 3 cut(s) 157, 543, 893
AsuII TTCGAA 1 cut(s) 540
AvaII GGWCC 3 cut(s) 431, 509, 673
BaeGI GKGCMC 1 cut(s) 669
BalI TGGCCA 1 cut(s) 870
BanI GGYRCC 1 cut(s) 316
BanII GRGCYC 1 cut(s) 69
BauI CACGAG 1 cut(s) 68
BbrPI CACGTG 1 cut(s) 234
BbsI GAAGAC 2 cut(s) 43, 198
Bbv12I GWGCWC 2 cut(s) 69, 669
BbvI GCAGC 3 cut(s) 255, 277, 1003
BccI CCATC 4 cut(s) 235, 419, 445, 860
BceAI ACGGC 1 cut(s) 705
BciT130I CCWGG 2 cut(s) 314, 987
BcoDI GTCTC 2 cut(s) 382, 551
BfaI CTAG 3 cut(s) 662, 804, 1178
BfoI RGCGCY 1 cut(s) 1028
BisI GCNGC 4 cut(s) 134, 269, 291, 1017
BlsI GCNGC 4 cut(s) 135, 270, 292, 1018
Bme1390I CCNGG 2 cut(s) 314, 987
Bme18I GGWCC 3 cut(s) 431, 509, 673
BmgBI CACGTC 1 cut(s) 809
BmgT120I GGNCC 5 cut(s) 135, 431, 509, 673, 1222
BmiI GGNNCC 4 cut(s) 318, 655, 674, 1223
BmrFI CCNGG 2 cut(s) 314, 987
BmrI ACTGGG 1 cut(s) 679
BmsI GCATC 1 cut(s) 535
BmuI ACTGGG 1 cut(s) 679
BpiI GAAGAC 2 cut(s) 43, 198
BpmI CTGGAG 1 cut(s) 44
Bpu14I TTCGAA 1 cut(s) 540
BpuEI CTTGAG 1 cut(s) 1105
BsaAI YACGTR 1 cut(s) 234
BsaBI GATNNNNATC 1 cut(s) 303
BsaJI CCNNGG 6 cut(s) 12, 150, 284, 788, 923, 1116
BsaWI WCCGGW 1 cut(s) 553
BsaXI ACNNNNNCTCC 2 cut(s) 496, 526
Bsc4I CCNNNNNNNGG 6 cut(s) 90, 472, 473, 506, 650, 1275
Bse1I ACTGG 3 cut(s) 511, 655, 674
Bse8I GATNNNNATC 1 cut(s) 303
BseBI CCWGG 2 cut(s) 314, 987
BseDI CCNNGG 6 cut(s) 12, 150, 284, 788, 923, 1116
BseGI GGATG 4 cut(s) 227, 586, 610, 871
BseJI GATNNNNATC 1 cut(s) 303
BseLI CCNNNNNNNGG 6 cut(s) 90, 472, 473, 506, 650, 1275
BseMII CTCAG 2 cut(s) 515, 1067
BseNI ACTGG 3 cut(s) 511, 655, 674
BseRI GAGGAG 1 cut(s) 431
BseSI GKGCMC 1 cut(s) 669
BseXI GCAGC 3 cut(s) 255, 277, 1003
BseYI CCCAGC 1 cut(s) 58
BsgI GTGCAG 1 cut(s) 864
Bsh1236I CGCG 1 cut(s) 1036
BshFI GGCC 6 cut(s) 11, 136, 720, 870, 990, 1224
BshNI GGYRCC 1 cut(s) 316
BsiHKAI GWGCWC 2 cut(s) 69, 669
BsiSI CCGG 1 cut(s) 554
BslI CCNNNNNNNGG 6 cut(s) 90, 472, 473, 506, 650, 1275
BsmAI GTCTC 2 cut(s) 382, 551
BsmI GAATGC 3 cut(s) 279, 644, 837
BsnI GGCC 6 cut(s) 11, 136, 720, 870, 990, 1224
Bsp119I TTCGAA 1 cut(s) 540
Bsp1286I GDGCHC 2 cut(s) 69, 669
Bsp143I GATC 9 cut(s) 454, 496, 743, 814, 1056, 1088, 1135, 1216, 1272
Bsp19I CCATGG 2 cut(s) 923, 1116
BspACI CCGC 7 cut(s) 133, 218, 222, 429, 676, 1036, 1142
BspANI GGCC 6 cut(s) 11, 136, 720, 870, 990, 1224
BspCNI CTCAG 2 cut(s) 514, 1066
BspFNI CGCG 1 cut(s) 1036
BspLI GGNNCC 4 cut(s) 318, 655, 674, 1223
BspPI GGATC 3 cut(s) 462, 491, 1130
BspT104I TTCGAA 1 cut(s) 540
BspT107I GGYRCC 1 cut(s) 316
BsrI ACTGG 3 cut(s) 511, 655, 674
BssECI CCNNGG 6 cut(s) 12, 150, 284, 788, 923, 1116
BssMI GATC 9 cut(s) 454, 496, 743, 814, 1056, 1088, 1135, 1216, 1272
BssSI CACGAG 1 cut(s) 68
BssT1I CCWWGG 4 cut(s) 284, 788, 923, 1116
Bst2BI CACGAG 1 cut(s) 68
Bst2UI CCWGG 2 cut(s) 314, 987
Bst4CI ACNGT 5 cut(s) 151, 535, 562, 755, 897
BstBAI YACGTR 1 cut(s) 234
BstBI TTCGAA 1 cut(s) 540
BstC8I GCNNGC 3 cut(s) 222, 872, 1207
BstDEI CTNAG 5 cut(s) 501, 762, 1053, 1107, 1233
BstDSI CCRYGG 3 cut(s) 150, 923, 1116
BstEII GGTNACC 1 cut(s) 145
BstF5I GGATG 4 cut(s) 227, 586, 610, 871
BstFNI CGCG 1 cut(s) 1036
BstH2I RGCGCY 1 cut(s) 1028
BstHHI GCGC 1 cut(s) 1027
BstKTI GATC 9 cut(s) 457, 499, 746, 817, 1059, 1091, 1138, 1219, 1275
BstMAI GTCTC 2 cut(s) 382, 551
BstMBI GATC 9 cut(s) 454, 496, 743, 814, 1056, 1088, 1135, 1216, 1272
BstMWI GCNNNNNNNGC 4 cut(s) 64, 1022, 1033, 1049
BstNI CCWGG 2 cut(s) 314, 987
BstPI GGTNACC 1 cut(s) 145
BstSCI CCNGG 2 cut(s) 312, 985
BstSLI GKGCMC 1 cut(s) 669
BstUI CGCG 1 cut(s) 1036
BstV1I GCAGC 3 cut(s) 255, 277, 1003
BstV2I GAAGAC 2 cut(s) 43, 198
BstX2I RGATCY 2 cut(s) 496, 1135
BstYI RGATCY 2 cut(s) 496, 1135
BsuRI GGCC 6 cut(s) 11, 136, 720, 870, 990, 1224
BtgI CCRYGG 3 cut(s) 150, 923, 1116
BtrI CACGTC 1 cut(s) 809
BtsCI GGATG 4 cut(s) 227, 586, 610, 871
BtsIMutI CAGTG 3 cut(s) 567, 667, 1078
Cac8I GCNNGC 3 cut(s) 222, 872, 1207
CfoI GCGC 1 cut(s) 1027
Cfr13I GGNCC 5 cut(s) 135, 431, 509, 673, 1222
Csp6I GTAC 1 cut(s) 820
CspCI CAANNNNNGTGG 4 cut(s) 31, 66, 941, 976
CviAII CATG 6 cut(s) 620, 658, 882, 905, 924, 1117
CviQI GTAC 1 cut(s) 820
DdeI CTNAG 5 cut(s) 501, 762, 1053, 1107, 1233
DpnI GATC 9 cut(s) 456, 498, 745, 816, 1058, 1090, 1137, 1218, 1274
DpnII GATC 9 cut(s) 454, 496, 743, 814, 1056, 1088, 1135, 1216, 1272
EaeI YGGCCR 1 cut(s) 868
EciI GGCGGA 1 cut(s) 207
Ecl136II GAGCTC 1 cut(s) 67
Eco130I CCWWGG 4 cut(s) 284, 788, 923, 1116
Eco147I AGGCCT 1 cut(s) 990
Eco24I GRGCYC 1 cut(s) 69
Eco32I GATATC 1 cut(s) 479
Eco47I GGWCC 3 cut(s) 431, 509, 673
Eco53kI GAGCTC 1 cut(s) 67
Eco72I CACGTG 1 cut(s) 234
Eco91I GGTNACC 1 cut(s) 145
EcoICRI GAGCTC 1 cut(s) 67
EcoO109I RGGNCCY 1 cut(s) 1222
EcoO65I GGTNACC 1 cut(s) 145
EcoRII CCWGG 2 cut(s) 312, 985
EcoRV GATATC 1 cut(s) 479
EcoT14I CCWWGG 4 cut(s) 284, 788, 923, 1116
EcoT38I GRGCYC 1 cut(s) 69
ErhI CCWWGG 4 cut(s) 284, 788, 923, 1116
FaeI CATG 6 cut(s) 623, 661, 885, 908, 927, 1120
FalI AAGNNNNNCTT 2 cut(s) 1014, 1046
FatI CATG 6 cut(s) 619, 657, 881, 904, 923, 1116
FauI CCCGC 1 cut(s) 229
FauNDI CATATG 2 cut(s) 401, 447
Fnu4HI GCNGC 4 cut(s) 134, 269, 291, 1017
FokI GGATG 4 cut(s) 214, 593, 617, 878
FriOI GRGCYC 1 cut(s) 69
Fsp4HI GCNGC 4 cut(s) 134, 269, 291, 1017
FspBI CTAG 3 cut(s) 662, 804, 1178
GlaI GCGC 1 cut(s) 1026
GluI GCNGC 4 cut(s) 134, 269, 291, 1017
GsaI CCCAGC 1 cut(s) 62
GsuI CTGGAG 1 cut(s) 44
HaeII RGCGCY 1 cut(s) 1028
HaeIII GGCC 6 cut(s) 11, 136, 720, 870, 990, 1224
HapII CCGG 1 cut(s) 554
HhaI GCGC 1 cut(s) 1027
Hin1II CATG 6 cut(s) 623, 661, 885, 908, 927, 1120
Hin6I GCGC 1 cut(s) 1025
HinP1I GCGC 1 cut(s) 1025
HincII GTYRAC 1 cut(s) 462
HindII GTYRAC 1 cut(s) 462
HindIII AAGCTT 1 cut(s) 483
HinfI GANTC 7 cut(s) 28, 191, 365, 442, 734, 851, 1112
HpaII CCGG 1 cut(s) 554
HphI GGTGA 3 cut(s) 157, 543, 893
Hpy166II GTNNAC 2 cut(s) 462, 667
Hpy188I TCNGA 6 cut(s) 309, 504, 1005, 1056, 1135, 1187
Hpy188III TCNNGA 4 cut(s) 70, 1214, 1228, 1276
Hpy8I GTNNAC 2 cut(s) 462, 667
Hpy99I CGWCG 1 cut(s) 726
HpyAV CCTTC 2 cut(s) 783, 1000
HpyCH4III ACNGT 5 cut(s) 151, 535, 562, 755, 897
HpyCH4IV ACGT 3 cut(s) 233, 808, 994
HpyCH4V TGCA 8 cut(s) 279, 339, 596, 667, 837, 845, 935, 1209
HpyF10VI GCNNNNNNNGC 4 cut(s) 64, 1022, 1033, 1049
HpyF3I CTNAG 5 cut(s) 501, 762, 1053, 1107, 1233
HpySE526I ACGT 3 cut(s) 233, 808, 994
Hsp92II CATG 6 cut(s) 623, 661, 885, 908, 927, 1120
HspAI GCGC 1 cut(s) 1025
Kzo9I GATC 9 cut(s) 454, 496, 743, 814, 1056, 1088, 1135, 1216, 1272
LmnI GCTCC 3 cut(s) 64, 182, 965
Lsp1109I GCAGC 3 cut(s) 255, 277, 1003
LweI GCATC 1 cut(s) 535
MaeI CTAG 3 cut(s) 662, 804, 1178
MaeII ACGT 3 cut(s) 233, 808, 994
MaeIII GTNAC 3 cut(s) 145, 757, 794
MalI GATC 9 cut(s) 456, 498, 745, 816, 1058, 1090, 1137, 1218, 1274
MboI GATC 9 cut(s) 454, 496, 743, 814, 1056, 1088, 1135, 1216, 1272
MboII GAAGA 9 cut(s) 48, 189, 198, 201, 374, 554, 647, 920, 1272
MflI RGATCY 2 cut(s) 496, 1135
MhlI GDGCHC 2 cut(s) 69, 669
MlsI TGGCCA 1 cut(s) 870
MluCI AATT 4 cut(s) 164, 274, 587, 1129
MluNI TGGCCA 1 cut(s) 870
MlyI GAGTC 1 cut(s) 200
MmeI TCCRAC 2 cut(s) 150, 1165
Mox20I TGGCCA 1 cut(s) 870
MscI TGGCCA 1 cut(s) 870
MseI TTAA 1 cut(s) 972
MslI CAYNNNNRTG 2 cut(s) 232, 662
Msp20I TGGCCA 1 cut(s) 870
MspA1I CMGCKG 1 cut(s) 1142
MspI CCGG 1 cut(s) 554
MspR9I CCNGG 2 cut(s) 314, 987
Mva1269I GAATGC 3 cut(s) 279, 644, 837
MvaI CCWGG 2 cut(s) 314, 987
MvnI CGCG 1 cut(s) 1036
MwoI GCNNNNNNNGC 4 cut(s) 64, 1022, 1033, 1049
NcoI CCATGG 2 cut(s) 923, 1116
NdeI CATATG 2 cut(s) 401, 447
NdeII GATC 9 cut(s) 454, 496, 743, 814, 1056, 1088, 1135, 1216, 1272
NlaIII CATG 6 cut(s) 623, 661, 885, 908, 927, 1120
NlaIV GGNNCC 4 cut(s) 318, 655, 674, 1223
NmuCI GTSAC 2 cut(s) 145, 794
NspV TTCGAA 1 cut(s) 540
PceI AGGCCT 1 cut(s) 990
PcsI WCGNNNNNNNCGW 1 cut(s) 114
PctI GAATGC 3 cut(s) 279, 644, 837
PfeI GAWTC 6 cut(s) 28, 365, 442, 734, 851, 1112
PkrI GCNGC 4 cut(s) 135, 270, 292, 1018
PleI GAGTC 1 cut(s) 199
PmaCI CACGTG 1 cut(s) 234
PmlI CACGTG 1 cut(s) 234
PpsI GAGTC 1 cut(s) 199
Ppu21I YACGTR 1 cut(s) 234
Psp124BI GAGCTC 1 cut(s) 69
Psp6I CCWGG 2 cut(s) 312, 985
PspCI CACGTG 1 cut(s) 234
PspEI GGTNACC 1 cut(s) 145
PspFI CCCAGC 1 cut(s) 58
PspGI CCWGG 2 cut(s) 312, 985
PspN4I GGNNCC 4 cut(s) 318, 655, 674, 1223
PspPI GGNCC 5 cut(s) 135, 431, 509, 673, 1222
PsrI GAACNNNNNNTAC 2 cut(s) 975, 1007
PsuI RGATCY 2 cut(s) 496, 1135
RsaI GTAC 1 cut(s) 821
RsaNI GTAC 1 cut(s) 820
RseI CAYNNNNRTG 2 cut(s) 232, 662
SacI GAGCTC 1 cut(s) 69
SaqAI TTAA 1 cut(s) 972
SatI GCNGC 4 cut(s) 134, 269, 291, 1017
Sau3AI GATC 9 cut(s) 454, 496, 743, 814, 1056, 1088, 1135, 1216, 1272
Sau96I GGNCC 5 cut(s) 135, 431, 509, 673, 1222
SchI GAGTC 1 cut(s) 200
ScrFI CCNGG 2 cut(s) 314, 987
SduI GDGCHC 2 cut(s) 69, 669
SfaNI GCATC 1 cut(s) 535
SfuI TTCGAA 1 cut(s) 540
SinI GGWCC 3 cut(s) 431, 509, 673
SmiMI CAYNNNNRTG 2 cut(s) 232, 662
SmlI CTYRAG 1 cut(s) 1084
SmoI CTYRAG 1 cut(s) 1084
Sse9I AATT 4 cut(s) 164, 274, 587, 1129
SseBI AGGCCT 1 cut(s) 990
SsiI CCGC 7 cut(s) 133, 218, 222, 429, 676, 1036, 1142
SspMI CTAG 3 cut(s) 662, 804, 1178
SstI GAGCTC 1 cut(s) 69
StuI AGGCCT 1 cut(s) 990
StyD4I CCNGG 2 cut(s) 312, 985
StyI CCWWGG 4 cut(s) 284, 788, 923, 1116
TaaI ACNGT 5 cut(s) 151, 535, 562, 755, 897
TaiI ACGT 3 cut(s) 236, 811, 997
TaqI TCGA 4 cut(s) 245, 251, 540, 1258
TaqII GACCGA 1 cut(s) 447
TasI AATT 4 cut(s) 164, 274, 587, 1129
TatI WGTACW 1 cut(s) 819
TauI GCSGC 1 cut(s) 136
TfiI GAWTC 6 cut(s) 28, 365, 442, 734, 851, 1112
Tru1I TTAA 1 cut(s) 972
Tru9I TTAA 1 cut(s) 972
TscAI CASTG 3 cut(s) 567, 674, 1078
TseFI GTSAC 2 cut(s) 145, 794
TseI GCWGC 3 cut(s) 268, 290, 1016
Tsp45I GTSAC 2 cut(s) 145, 794
TspDTI ATGAA 7 cut(s) 67, 201, 434, 600, 608, 870, 921
TspRI CASTG 3 cut(s) 567, 674, 1078
VneI GTGCAC 1 cut(s) 665
VpaK11BI GGWCC 3 cut(s) 431, 509, 673
XapI RAATTY 2 cut(s) 587, 1129
XspI CTAG 3 cut(s) 662, 804, 1178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.