MD07G1298700.v1.1

START domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0003829
Physical Location & Seq
Reverse (-)
35816308 .. 35818940
2633 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1298700.v1.1.491

Sequence Viewer

Length: 1260 bp
ATGGAGGTCGCAGGTTTGTATTTGATGGTGCTTCTGGGTATTTCGTTGGCATTGCTTGTTGGAGTTTTGATCGGATGGTCGTGGAAACCAAAGTGGGCATTCTCTGGCAATGACAAGGACAAACTAGGCTGCCCTGTGCCTAACGCTTTTGATTTTTCGTCAGCTCTATGCTTGAATTGTTTTGAGTCATTGGTTTCCCAAGGTTGTGAAGCTCGCTGCATGGGTAAGGGAATTCAGAAAACTCCTTCTGTATCGCCTCCGGCTACCGAATTCGATGAGGGCAGCAGCACATCGCAGCTGAATGAGGAGAAGCCGACTGTAGTGACTGAGGAGGATTTAGAGGTTTTATCCGAGCTTGTAGATATGAAAGATGGAGGTCCTCCATGGATACAGATGATGGATCGCTCCACCCCAACTATGACCTACCAAGGCTGGCGTAGGGATCCAAAGGTCGGTCCTCCTCAATATCGTAGCAGAACTGTCTACGAGGATGCAACGCCGGAATTAGTGAGGGATTTCTTCTGGGATGACGAGTTTCGACTAAGGTGGGATGACATGCTTAGTTATGCGAAAACCATAGAAGAATGCCCGACCACAGGAACCATGGTGACGCTGTGGATTAAGAAGTTCCCGTTTTTCTGTAAAGACAGAGAATACATCATAGGTCGTCGAATTTGGGAATCTGGGAGGTTGTATTATTGCGTGACAAAGGGAGTGCCCTATGCTTCTGTTCCAAGACATGACAAGCAAGTACGTGTAGATCTGTACTTCTCAAGTTGGTGCATTCAAGCAGTGGAATCAAGAAGGGCTGACGGCCAGCTGACTGCGTGTGAGGTTATATTCTTACATTACGAAGATAATGGACTCCCATGGGAAATTGTAAAACTTGGAATAAGAACAGGGATGTGGGATACAGTCAAGAAGATTGAGGCTGGCTTACGCACCTACCAGAAGGAAAGAGCAGCAGGATCACCACTTTCCCGGCCAGCCTTTATGGCTCAGATCAACACGAAAATACATCCCGGGTACCTGAAATCCTCAGGAGCCACAGAAGACTCATCACAAACTGAGGTGGTGATTGCATCCAAGAAGCCTGCGGGTAGGCACATACCCAAGCTCTTAATACTTGGGGGGACAATAATCATTGCTTGCACTCTTAATAAGGGACACCTGATGAAGGTGGTGATATTTGGGGTAGGCGGAAAGCTTGCAAAGGTAGGAAAGAAATTGCAAACAAAGAGTGATCGAGATTCAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
START

Protein Analysis

420

Amino Acids

47.01

Weight (kDa)

8.36

Isoelectric Point (pI)

47.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
START PF01852 122 - 316 6.2e-08 START domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2
Acc65I GGTACC 1 cut(s) 1026
AccB1I GGYRCC 1 cut(s) 1026
AccI GTMKAC 1 cut(s) 483
AciI CCGC 2 cut(s) 1097, 1200
AclWI GGATC 4 cut(s) 408, 437, 450, 976
AcoI YGGCCR 2 cut(s) 814, 983
AcsI RAATTY 3 cut(s) 231, 269, 672
AfaI GTAC 3 cut(s) 753, 767, 1028
AfiI CCNNNNNNNGG 3 cut(s) 452, 596, 1177
AflIII ACRYGT 1 cut(s) 754
AgsI TTSAA 3 cut(s) 175, 788, 1254
AluBI AGCT 7 cut(s) 164, 212, 298, 355, 820, 1117, 1207
AluI AGCT 7 cut(s) 164, 212, 298, 355, 820, 1117, 1207
AlwI GGATC 4 cut(s) 408, 437, 450, 976
Ama87I CYCGRG 1 cut(s) 1022
AoxI GGCC 2 cut(s) 814, 983
ApeKI GCWGC 6 cut(s) 129, 216, 282, 285, 295, 962
ApoI RAATTY 3 cut(s) 231, 269, 672
Asp718I GGTACC 1 cut(s) 1026
AspS9I GGNCC 2 cut(s) 377, 455
AsuC2I CCSGG 3 cut(s) 982, 1023, 1024
AsuHPI GGTGA 4 cut(s) 619, 963, 1087, 1195
AvaI CYCGRG 1 cut(s) 1022
AvaII GGWCC 2 cut(s) 377, 455
AxyI CCTNAGG 1 cut(s) 1039
BaeGI GKGCMC 1 cut(s) 720
BamHI GGATCC 1 cut(s) 442
BanI GGYRCC 1 cut(s) 1026
BbsI GAAGAC 1 cut(s) 1059
BbvI GCAGC 6 cut(s) 116, 203, 294, 297, 307, 974
BccI CCATC 4 cut(s) 19, 69, 365, 391
BceAI ACGGC 1 cut(s) 829
BciVI GTATCC 2 cut(s) 381, 904
BcnI CCSGG 3 cut(s) 982, 1023, 1024
BfaI CTAG 1 cut(s) 125
BfmI CTRYAG 1 cut(s) 318
BfuAI ACCTGC 1 cut(s) 2
BfuI GTATCC 2 cut(s) 381, 904
BglI GCCNNNNNGGC 1 cut(s) 995
BglII AGATCT 1 cut(s) 760
BisI GCNGC 6 cut(s) 130, 217, 283, 286, 296, 963
BlsI GCNGC 6 cut(s) 131, 218, 284, 287, 297, 964
Bme1390I CCNGG 3 cut(s) 982, 1023, 1024
Bme18I GGWCC 2 cut(s) 377, 455
BmeT110I CYCGRG 1 cut(s) 1022
BmgT120I GGNCC 2 cut(s) 377, 455
BmiI GGNNCC 4 cut(s) 444, 601, 1028, 1045
BmrFI CCNGG 3 cut(s) 982, 1023, 1024
BmsI GCATC 2 cut(s) 481, 1091
BpiI GAAGAC 1 cut(s) 1059
BpuEI CTTGAG 1 cut(s) 757
BpuMI CCSGG 3 cut(s) 982, 1023, 1024
BsaAI YACGTR 1 cut(s) 755
BsaJI CCNNGG 6 cut(s) 199, 383, 427, 603, 869, 1022
Bsc4I CCNNNNNNNGG 3 cut(s) 452, 596, 1177
Bse21I CCTNAGG 1 cut(s) 1039
Bse3DI GCAATG 3 cut(s) 50, 115, 1143
BseDI CCNNGG 6 cut(s) 199, 383, 427, 603, 869, 1022
BseGI GGATG 7 cut(s) 80, 496, 532, 556, 909, 1018, 1082
BseLI CCNNNNNNNGG 3 cut(s) 452, 596, 1177
BseMI GCAATG 3 cut(s) 50, 115, 1143
BseMII CTCAG 4 cut(s) 318, 1013, 1053, 1059
BseRI GAGGAG 3 cut(s) 320, 344, 450
BseSI GKGCMC 1 cut(s) 720
BseXI GCAGC 6 cut(s) 116, 203, 294, 297, 307, 974
BshFI GGCC 2 cut(s) 816, 985
BshNI GGYRCC 1 cut(s) 1026
BsiHKCI CYCGRG 1 cut(s) 1022
BsiSI CCGG 4 cut(s) 260, 500, 982, 1023
BslFI GGGAC 2 cut(s) 1147, 1179
BslI CCNNNNNNNGG 3 cut(s) 452, 596, 1177
BsmFI GGGAC 2 cut(s) 1147, 1179
BsmI GAATGC 3 cut(s) 98, 590, 783
BsnI GGCC 2 cut(s) 816, 985
BsoBI CYCGRG 1 cut(s) 1022
Bsp1286I GDGCHC 1 cut(s) 720
Bsp143I GATC 7 cut(s) 69, 400, 442, 760, 968, 1002, 1243
Bsp19I CCATGG 3 cut(s) 383, 603, 869
BspACI CCGC 2 cut(s) 1097, 1200
BspANI GGCC 2 cut(s) 816, 985
BspCNI CTCAG 4 cut(s) 319, 1012, 1052, 1060
BspLI GGNNCC 4 cut(s) 444, 601, 1028, 1045
BspMI ACCTGC 1 cut(s) 2
BspPI GGATC 4 cut(s) 408, 437, 450, 976
BspT107I GGYRCC 1 cut(s) 1026
BsrDI GCAATG 3 cut(s) 50, 115, 1143
BssECI CCNNGG 6 cut(s) 199, 383, 427, 603, 869, 1022
BssMI GATC 7 cut(s) 69, 400, 442, 760, 968, 1002, 1243
BssT1I CCWWGG 5 cut(s) 199, 383, 427, 603, 869
Bst4CI ACNGT 3 cut(s) 319, 481, 916
BstBAI YACGTR 1 cut(s) 755
BstC8I GCNNGC 8 cut(s) 214, 434, 818, 934, 987, 1095, 1150, 1209
BstDEI CTNAG 6 cut(s) 327, 542, 560, 999, 1039, 1068
BstDSI CCRYGG 3 cut(s) 383, 603, 869
BstENI CCTNNNNNAGG 1 cut(s) 1175
BstF5I GGATG 7 cut(s) 80, 496, 532, 556, 909, 1018, 1082
BstKTI GATC 7 cut(s) 72, 403, 445, 763, 971, 1005, 1246
BstMBI GATC 7 cut(s) 69, 400, 442, 760, 968, 1002, 1243
BstMWI GCNNNNNNNGC 1 cut(s) 995
BstNSI RCATGY 1 cut(s) 559
BstSCI CCNGG 3 cut(s) 980, 1021, 1022
BstSFI CTRYAG 1 cut(s) 318
BstSLI GKGCMC 1 cut(s) 720
BstV1I GCAGC 6 cut(s) 116, 203, 294, 297, 307, 974
BstV2I GAAGAC 1 cut(s) 1059
BstX2I RGATCY 2 cut(s) 442, 760
BstYI RGATCY 2 cut(s) 442, 760
Bsu36I CCTNAGG 1 cut(s) 1039
BsuI GTATCC 2 cut(s) 381, 904
BsuRI GGCC 2 cut(s) 816, 985
BtgI CCRYGG 3 cut(s) 383, 603, 869
BtgZI GCGATG 1 cut(s) 276
BtsCI GGATG 7 cut(s) 80, 496, 532, 556, 909, 1018, 1082
BtsI GCAGTG 1 cut(s) 798
BtsIMutI CAGTG 1 cut(s) 798
BveI ACCTGC 1 cut(s) 2
Cac8I GCNNGC 8 cut(s) 214, 434, 818, 934, 987, 1095, 1150, 1209
Cfr13I GGNCC 2 cut(s) 377, 455
Cfr9I CCCGGG 1 cut(s) 1022
CseI GACGC 1 cut(s) 619
Csp6I GTAC 3 cut(s) 752, 766, 1027
CviAII CATG 6 cut(s) 220, 384, 556, 604, 740, 870
CviQI GTAC 3 cut(s) 752, 766, 1027
DdeI CTNAG 6 cut(s) 327, 542, 560, 999, 1039, 1068
DpnI GATC 7 cut(s) 71, 402, 444, 762, 970, 1004, 1245
DpnII GATC 7 cut(s) 69, 400, 442, 760, 968, 1002, 1243
EaeI YGGCCR 2 cut(s) 814, 983
EciI GGCGGA 1 cut(s) 1215
Eco130I CCWWGG 5 cut(s) 199, 383, 427, 603, 869
Eco47I GGWCC 2 cut(s) 377, 455
Eco81I CCTNAGG 1 cut(s) 1039
Eco88I CYCGRG 1 cut(s) 1022
EcoNI CCTNNNNNAGG 1 cut(s) 1175
EcoO109I RGGNCCY 1 cut(s) 377
EcoRI GAATTC 2 cut(s) 231, 269
EcoT14I CCWWGG 5 cut(s) 199, 383, 427, 603, 869
ErhI CCWWGG 5 cut(s) 199, 383, 427, 603, 869
FaeI CATG 6 cut(s) 223, 387, 559, 607, 743, 873
FaqI GGGAC 2 cut(s) 1147, 1179
FatI CATG 6 cut(s) 219, 383, 555, 603, 739, 869
FauI CCCGC 1 cut(s) 1090
FblI GTMKAC 1 cut(s) 483
Fnu4HI GCNGC 6 cut(s) 130, 217, 283, 286, 296, 963
FokI GGATG 7 cut(s) 87, 503, 539, 563, 916, 1005, 1069
Fsp4HI GCNGC 6 cut(s) 130, 217, 283, 286, 296, 963
FspBI CTAG 1 cut(s) 125
GluI GCNGC 6 cut(s) 130, 217, 283, 286, 296, 963
HaeIII GGCC 2 cut(s) 816, 985
HapII CCGG 4 cut(s) 260, 500, 982, 1023
HgaI GACGC 1 cut(s) 619
Hin1II CATG 6 cut(s) 223, 387, 559, 607, 743, 873
HindIII AAGCTT 1 cut(s) 1205
HinfI GANTC 6 cut(s) 185, 680, 797, 864, 1055, 1250
HpaII CCGG 4 cut(s) 260, 500, 982, 1023
HphI GGTGA 4 cut(s) 619, 963, 1087, 1195
Hpy166II GTNNAC 1 cut(s) 484
Hpy188I TCNGA 4 cut(s) 74, 237, 352, 1002
Hpy188III TCNNGA 4 cut(s) 801, 919, 1041, 1247
Hpy8I GTNNAC 1 cut(s) 484
Hpy99I CGWCG 1 cut(s) 672
HpyAV CCTTC 4 cut(s) 255, 798, 946, 1171
HpyCH4III ACNGT 3 cut(s) 319, 481, 916
HpyCH4IV ACGT 1 cut(s) 754
HpyCH4V TGCA 7 cut(s) 219, 494, 783, 1082, 1152, 1211, 1231
HpyF10VI GCNNNNNNNGC 1 cut(s) 995
HpyF3I CTNAG 6 cut(s) 327, 542, 560, 999, 1039, 1068
HpySE526I ACGT 1 cut(s) 754
Hsp92II CATG 6 cut(s) 223, 387, 559, 607, 743, 873
KpnI GGTACC 1 cut(s) 1030
Kzo9I GATC 7 cut(s) 69, 400, 442, 760, 968, 1002, 1243
LmnI GCTCC 2 cut(s) 410, 1043
Lsp1109I GCAGC 6 cut(s) 116, 203, 294, 297, 307, 974
LweI GCATC 2 cut(s) 481, 1091
MaeI CTAG 1 cut(s) 125
MaeII ACGT 1 cut(s) 754
MaeIII GTNAC 3 cut(s) 322, 607, 703
MalI GATC 7 cut(s) 71, 402, 444, 762, 970, 1004, 1245
MboI GATC 7 cut(s) 69, 400, 442, 760, 968, 1002, 1243
MboII GAAGA 5 cut(s) 511, 593, 866, 934, 1064
MflI RGATCY 2 cut(s) 442, 760
MhlI GDGCHC 1 cut(s) 720
MluCI AATT 7 cut(s) 175, 231, 269, 503, 672, 876, 1226
MlyI GAGTC 3 cut(s) 194, 858, 1049
MmeI TCCRAC 1 cut(s) 40
MseI TTAA 3 cut(s) 621, 1121, 1158
MspA1I CMGCKG 2 cut(s) 298, 820
MspI CCGG 4 cut(s) 260, 500, 982, 1023
MspR9I CCNGG 3 cut(s) 982, 1023, 1024
Mva1269I GAATGC 3 cut(s) 98, 590, 783
MwoI GCNNNNNNNGC 1 cut(s) 995
NciI CCSGG 3 cut(s) 982, 1023, 1024
NcoI CCATGG 3 cut(s) 383, 603, 869
NdeII GATC 7 cut(s) 69, 400, 442, 760, 968, 1002, 1243
NlaIII CATG 6 cut(s) 223, 387, 559, 607, 743, 873
NlaIV GGNNCC 4 cut(s) 444, 601, 1028, 1045
NmuCI GTSAC 3 cut(s) 322, 607, 703
NspI RCATGY 1 cut(s) 559
PctI GAATGC 3 cut(s) 98, 590, 783
PfeI GAWTC 3 cut(s) 680, 797, 1250
PkrI GCNGC 6 cut(s) 131, 218, 284, 287, 297, 964
PleI GAGTC 3 cut(s) 193, 858, 1049
PpsI GAGTC 3 cut(s) 193, 858, 1049
Ppu21I YACGTR 1 cut(s) 755
PpuMI RGGWCCY 1 cut(s) 377
Psp5II RGGWCCY 1 cut(s) 377
PspN4I GGNNCC 4 cut(s) 444, 601, 1028, 1045
PspPI GGNCC 2 cut(s) 377, 455
PspPPI RGGWCCY 1 cut(s) 377
PsuI RGATCY 2 cut(s) 442, 760
PvuII CAGCTG 2 cut(s) 298, 820
RsaI GTAC 3 cut(s) 753, 767, 1028
RsaNI GTAC 3 cut(s) 752, 766, 1027
SaqAI TTAA 3 cut(s) 621, 1121, 1158
SatI GCNGC 6 cut(s) 130, 217, 283, 286, 296, 963
Sau3AI GATC 7 cut(s) 69, 400, 442, 760, 968, 1002, 1243
Sau96I GGNCC 2 cut(s) 377, 455
SchI GAGTC 3 cut(s) 194, 858, 1049
ScrFI CCNGG 3 cut(s) 982, 1023, 1024
SduI GDGCHC 1 cut(s) 720
SfaNI GCATC 2 cut(s) 481, 1091
SfcI CTRYAG 1 cut(s) 318
SinI GGWCC 2 cut(s) 377, 455
SmaI CCCGGG 1 cut(s) 1024
SmlI CTYRAG 1 cut(s) 772
SmoI CTYRAG 1 cut(s) 772
Sse9I AATT 7 cut(s) 175, 231, 269, 503, 672, 876, 1226
SsiI CCGC 2 cut(s) 1097, 1200
SspMI CTAG 1 cut(s) 125
StyD4I CCNGG 3 cut(s) 980, 1021, 1022
StyI CCWWGG 5 cut(s) 199, 383, 427, 603, 869
TaaI ACNGT 3 cut(s) 319, 481, 916
TaiI ACGT 1 cut(s) 757
TaqI TCGA 4 cut(s) 273, 538, 670, 1246
TaqII GACCGA 1 cut(s) 443
TasI AATT 7 cut(s) 175, 231, 269, 503, 672, 876, 1226
TatI WGTACW 1 cut(s) 765
TfiI GAWTC 3 cut(s) 680, 797, 1250
Tru1I TTAA 3 cut(s) 621, 1121, 1158
Tru9I TTAA 3 cut(s) 621, 1121, 1158
TscAI CASTG 1 cut(s) 798
TseFI GTSAC 3 cut(s) 322, 607, 703
TseI GCWGC 6 cut(s) 129, 216, 282, 285, 295, 962
Tsp45I GTSAC 3 cut(s) 322, 607, 703
TspDTI ATGAA 2 cut(s) 380, 1190
TspMI CCCGGG 1 cut(s) 1022
TspRI CASTG 1 cut(s) 798
VpaK11BI GGWCC 2 cut(s) 377, 455
XagI CCTNNNNNAGG 1 cut(s) 1175
XapI RAATTY 3 cut(s) 231, 269, 672
XceI RCATGY 1 cut(s) 559
XcmI CCANNNNNNNNNTGG 1 cut(s) 601
XmaI CCCGGG 1 cut(s) 1022
XmiI GTMKAC 1 cut(s) 483
XspI CTAG 1 cut(s) 125
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.