Rh1DG450400

START domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0003829
Physical Location & Seq
Reverse (-)
65838809 .. 65842333
3525 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG450400.1

Sequence Viewer

Length: 1077 bp
ATGCCTGCTTCGCCGGCTAGGTCTGTGATGTCACCACCCTTAAAAGGGTTCTCATCAGCACCGTGCTTGTTCCAAGGCTATGAAGCTTGGTTCATTAACCATGCAGTGCAGAAGAGGCTTTCTCCCCCGCCTATCGAATTTGATGACCACAGTACCTGTACATCGCAGATGAATGAGAAGAAGTCTAATGTTGTGGCTCAGGAGGATGTAGAAAATCTATGTCGGCTTGTAGATATGAAAGATGGAGGTCCGGCTTGGATACATATGATGGATCGTTCAACTCCAACTATGGCCTACCAAGCTTGGCGTAGGGATCCAAAGATTGGTCCTCCTCAATATCGTAGCAGGATTGTGTATGAGGATGCAACTCCGGAGCTAGTGAGGGACTTTTTATGGGATGATGAGTTCCGATTAAGGTGGGACGACATGCTTAGTGATGCCACAACCTTAGATGAGTGCCCAACCACAGGAACCATGTTCTCGTTCTTCTTTAAAGACAGGGAATACATAATAGGACGTCGAATTTGGGAGTCAGGAAGGAAGTATTATTGTATGACAAAGGGAGTACCCTATCCTTCTATTCAAAGGCATGACAAACCAAGACGTGTGGATCACTACTACTCAAATTGGTGCATTCAAGCAGTGGAATCAAGACACCGCAATGGCCAACTGACTGCATGTGAAGTTCAACTGTTTCATCAAGAGGATATGGGAATCCCATGGAAAATTGCAAAACTTGTTGTACGAAAAGGAATGTGGGATACTAAAGAGAGAGCGTTAGATCAAAAGGAATGTGGAATAATTTCTCGGCCTACCTTTCTGGCTCAGATCAACACCAAAATAAATCTAGAGTACCTGAAAGCCTCCGCTAGCACTGAGGATTCGTCACAAACTGCAGTGGTGACTACATCTGACAAACCTTTCGGTGGGAACATACGGAATCTCATAATTGTTGGTGGGAGTATTCTCCTTGCATGCACTCTTGACCGGGGGTTTCTGGTGAAGACAGTAATATTCGGTGTGGCCGATGTGGCCAGGAAGTTTGCAAATGTGGGGAAGAAGTCAAAACCAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
START

Protein Analysis

358

Amino Acids

40.95

Weight (kDa)

8.85

Isoelectric Point (pI)

50.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 520
AccB7I CCANNNNNTGG 1 cut(s) 323
AccIII TCCGGA 1 cut(s) 370
AciI CCGC 3 cut(s) 128, 658, 867
AclWI GGATC 4 cut(s) 279, 308, 321, 618
AcoI YGGCCR 3 cut(s) 664, 1023, 1032
AcsI RAATTY 2 cut(s) 137, 522
AcyI GRCGYC 1 cut(s) 517
AfaI GTAC 5 cut(s) 154, 160, 567, 744, 854
AfiI CCNNNNNNNGG 5 cut(s) 44, 45, 323, 467, 926
AflIII ACRYGT 1 cut(s) 604
AgsI TTSAA 4 cut(s) 279, 584, 638, 689
AjiI CACGTC 1 cut(s) 605
AjnI CCWGG 1 cut(s) 1034
AloI GAACNNNNNNTCC 2 cut(s) 389, 421
AluBI AGCT 3 cut(s) 86, 302, 376
AluI AGCT 3 cut(s) 86, 302, 376
AlwI GGATC 4 cut(s) 279, 308, 321, 618
AlwNI CAGNNNCTG 1 cut(s) 156
Aor13HI TCCGGA 1 cut(s) 370
AoxI GGCC 5 cut(s) 291, 664, 809, 1023, 1032
ApoI RAATTY 2 cut(s) 137, 522
ArsI GACNNNNNNTTYG 4 cut(s) 507, 539, 905, 937
Asp700I GAANNNNTTC 1 cut(s) 802
AspS9I GGNCC 2 cut(s) 248, 326
AsuC2I CCSGG 1 cut(s) 989
AsuHPI GGTGA 3 cut(s) 24, 913, 1012
AsuNHI GCTAGC 1 cut(s) 869
AvaII GGWCC 2 cut(s) 248, 326
BaeGI GKGCMC 1 cut(s) 461
BaeI ACNNNNGTAYC 2 cut(s) 136, 169
BalI TGGCCA 2 cut(s) 666, 1034
BamHI GGATCC 1 cut(s) 313
BbsI GAAGAC 1 cut(s) 1010
BccI CCATC 2 cut(s) 236, 262
BciT130I CCWGG 1 cut(s) 1036
BciVI GTATCC 2 cut(s) 252, 754
BcnI CCSGG 1 cut(s) 989
BfaI CTAG 4 cut(s) 18, 377, 848, 870
BfmI CTRYAG 1 cut(s) 894
BfuI GTATCC 2 cut(s) 252, 754
BglI GCCNNNNNGGC 1 cut(s) 1031
Bme1390I CCNGG 2 cut(s) 989, 1036
Bme18I GGWCC 2 cut(s) 248, 326
BmgBI CACGTC 1 cut(s) 605
BmgT120I GGNCC 2 cut(s) 248, 326
BmiI GGNNCC 2 cut(s) 315, 472
BmrFI CCNGG 2 cut(s) 989, 1036
BmsI GCATC 2 cut(s) 352, 427
BmtI GCTAGC 1 cut(s) 873
BpiI GAAGAC 1 cut(s) 1010
BplI GAGNNNNNCTC 2 cut(s) 106, 138
Bpu10I CCTNAGC 1 cut(s) 198
BpuMI CCSGG 1 cut(s) 989
BsaHI GRCGYC 1 cut(s) 517
BsaJI CCNNGG 3 cut(s) 73, 719, 988
BsaWI WCCGGW 1 cut(s) 370
Bsc4I CCNNNNNNNGG 5 cut(s) 44, 45, 323, 467, 926
Bse118I RCCGGY 1 cut(s) 13
Bse3DI GCAATG 1 cut(s) 667
BseAI TCCGGA 1 cut(s) 370
BseBI CCWGG 1 cut(s) 1036
BseDI CCNNGG 3 cut(s) 73, 719, 988
BseGI GGATG 3 cut(s) 211, 367, 403
BseLI CCNNNNNNNGG 5 cut(s) 44, 45, 323, 467, 926
BseMI GCAATG 1 cut(s) 667
BseMII CTCAG 3 cut(s) 212, 839, 867
BseRI GAGGAG 1 cut(s) 321
BseSI GKGCMC 1 cut(s) 461
BsgI GTGCAG 1 cut(s) 128
BshFI GGCC 5 cut(s) 293, 666, 811, 1025, 1034
BsiSI CCGG 4 cut(s) 14, 251, 371, 988
BslFI GGGAC 2 cut(s) 398, 434
BslI CCNNNNNNNGG 5 cut(s) 44, 45, 323, 467, 926
BsmFI GGGAC 2 cut(s) 398, 434
BsmI GAATGC 1 cut(s) 633
BsnI GGCC 5 cut(s) 293, 666, 811, 1025, 1034
Bsp1286I GDGCHC 1 cut(s) 461
Bsp13I TCCGGA 1 cut(s) 370
Bsp1407I TGTACA 1 cut(s) 158
Bsp143I GATC 5 cut(s) 271, 313, 610, 781, 828
Bsp19I CCATGG 1 cut(s) 719
BspACI CCGC 3 cut(s) 128, 658, 867
BspANI GGCC 5 cut(s) 293, 666, 811, 1025, 1034
BspCNI CTCAG 3 cut(s) 211, 838, 868
BspEI TCCGGA 1 cut(s) 370
BspLI GGNNCC 2 cut(s) 315, 472
BspMAI CTGCAG 1 cut(s) 898
BspOI GCTAGC 1 cut(s) 873
BspPI GGATC 4 cut(s) 279, 308, 321, 618
BsrDI GCAATG 1 cut(s) 667
BsrFI RCCGGY 1 cut(s) 13
BsrGI TGTACA 1 cut(s) 158
BssAI RCCGGY 1 cut(s) 13
BssECI CCNNGG 3 cut(s) 73, 719, 988
BssMI GATC 5 cut(s) 271, 313, 610, 781, 828
BssNI GRCGYC 1 cut(s) 517
BssT1I CCWWGG 2 cut(s) 73, 719
Bst2UI CCWGG 1 cut(s) 1036
Bst4CI ACNGT 4 cut(s) 63, 152, 693, 1009
Bst6I CTCTTC 1 cut(s) 107
BstACI GRCGYC 1 cut(s) 517
BstAUI TGTACA 1 cut(s) 158
BstC8I GCNNGC 4 cut(s) 6, 15, 871, 976
BstDEI CTNAG 5 cut(s) 198, 431, 448, 825, 876
BstDSI CCRYGG 1 cut(s) 719
BstF5I GGATG 3 cut(s) 211, 367, 403
BstKTI GATC 5 cut(s) 274, 316, 613, 784, 831
BstMBI GATC 5 cut(s) 271, 313, 610, 781, 828
BstMWI GCNNNNNNNGC 5 cut(s) 10, 14, 115, 299, 1031
BstNI CCWGG 1 cut(s) 1036
BstNSI RCATGY 3 cut(s) 430, 681, 978
BstSCI CCNGG 2 cut(s) 987, 1034
BstSFI CTRYAG 1 cut(s) 894
BstSLI GKGCMC 1 cut(s) 461
BstV2I GAAGAC 1 cut(s) 1010
BstX2I RGATCY 1 cut(s) 313
BstYI RGATCY 1 cut(s) 313
BsuI GTATCC 2 cut(s) 252, 754
BsuRI GGCC 5 cut(s) 293, 666, 811, 1025, 1034
BtgI CCRYGG 1 cut(s) 719
BtgZI GCGATG 1 cut(s) 147
BtrI CACGTC 1 cut(s) 605
BtsCI GGATG 3 cut(s) 211, 367, 403
BtsI GCAGTG 3 cut(s) 111, 648, 903
BtsIMutI CAGTG 4 cut(s) 111, 648, 873, 903
Cac8I GCNNGC 4 cut(s) 6, 15, 871, 976
CaiI CAGNNNCTG 1 cut(s) 156
Cfr10I RCCGGY 1 cut(s) 13
Cfr13I GGNCC 2 cut(s) 248, 326
Csp6I GTAC 5 cut(s) 153, 159, 566, 743, 853
CspCI CAANNNNNGTGG 2 cut(s) 588, 623
CviAII CATG 7 cut(s) 101, 427, 475, 590, 678, 720, 975
CviQI GTAC 5 cut(s) 153, 159, 566, 743, 853
DdeI CTNAG 5 cut(s) 198, 431, 448, 825, 876
DpnI GATC 5 cut(s) 273, 315, 612, 783, 830
DpnII GATC 5 cut(s) 271, 313, 610, 781, 828
DraI TTTAAA 1 cut(s) 493
EaeI YGGCCR 3 cut(s) 664, 1023, 1032
Eam1104I CTCTTC 1 cut(s) 107
EarI CTCTTC 1 cut(s) 107
Eco130I CCWWGG 2 cut(s) 73, 719
Eco47I GGWCC 2 cut(s) 248, 326
EcoRII CCWGG 1 cut(s) 1034
EcoT14I CCWWGG 2 cut(s) 73, 719
ErhI CCWWGG 2 cut(s) 73, 719
FaeI CATG 7 cut(s) 104, 430, 478, 593, 681, 723, 978
FaqI GGGAC 2 cut(s) 398, 434
FatI CATG 7 cut(s) 100, 426, 474, 589, 677, 719, 974
FauI CCCGC 1 cut(s) 135
FauNDI CATATG 1 cut(s) 264
FokI GGATG 3 cut(s) 218, 374, 410
FspBI CTAG 4 cut(s) 18, 377, 848, 870
HaeIII GGCC 5 cut(s) 293, 666, 811, 1025, 1034
HapII CCGG 4 cut(s) 14, 251, 371, 988
Hin1I GRCGYC 1 cut(s) 517
Hin1II CATG 7 cut(s) 104, 430, 478, 593, 681, 723, 978
HindIII AAGCTT 2 cut(s) 84, 300
HinfI GANTC 5 cut(s) 530, 647, 714, 881, 940
HpaII CCGG 4 cut(s) 14, 251, 371, 988
HphI GGTGA 3 cut(s) 24, 913, 1012
Hpy188I TCNGA 3 cut(s) 410, 828, 913
Hpy188III TCNNGA 7 cut(s) 200, 371, 534, 651, 701, 848, 983
Hpy99I CGWCG 1 cut(s) 522
HpyAV CCTTC 2 cut(s) 531, 585
HpyCH4III ACNGT 4 cut(s) 63, 152, 693, 1009
HpyCH4IV ACGT 2 cut(s) 517, 604
HpyF10VI GCNNNNNNNGC 5 cut(s) 10, 14, 115, 299, 1031
HpyF3I CTNAG 5 cut(s) 198, 431, 448, 825, 876
HpySE526I ACGT 2 cut(s) 517, 604
Hsp92I GRCGYC 1 cut(s) 517
Hsp92II CATG 7 cut(s) 104, 430, 478, 593, 681, 723, 978
Kpn2I TCCGGA 1 cut(s) 370
KroI GCCGGC 1 cut(s) 13
KroNI GCCGGC 1 cut(s) 15
Kzo9I GATC 5 cut(s) 271, 313, 610, 781, 828
LmnI GCTCC 1 cut(s) 373
LweI GCATC 2 cut(s) 352, 427
MaeI CTAG 4 cut(s) 18, 377, 848, 870
MaeII ACGT 2 cut(s) 517, 604
MaeIII GTNAC 3 cut(s) 30, 885, 901
MalI GATC 5 cut(s) 273, 315, 612, 783, 830
MboI GATC 5 cut(s) 271, 313, 610, 781, 828
MboII GAAGA 5 cut(s) 124, 190, 478, 1015, 1069
MflI RGATCY 1 cut(s) 313
MhlI GDGCHC 1 cut(s) 461
MlsI TGGCCA 2 cut(s) 666, 1034
MluCI AATT 6 cut(s) 137, 522, 625, 726, 801, 948
MluNI TGGCCA 2 cut(s) 666, 1034
MlyI GAGTC 1 cut(s) 539
MmeI TCCRAC 1 cut(s) 308
Mox20I TGGCCA 2 cut(s) 666, 1034
MroI TCCGGA 1 cut(s) 370
MroNI GCCGGC 1 cut(s) 13
MroXI GAANNNNTTC 1 cut(s) 802
MscI TGGCCA 2 cut(s) 666, 1034
MseI TTAA 4 cut(s) 41, 96, 413, 492
MslI CAYNNNNRTG 1 cut(s) 660
Msp20I TGGCCA 2 cut(s) 666, 1034
MspI CCGG 4 cut(s) 14, 251, 371, 988
MspR9I CCNGG 2 cut(s) 989, 1036
Mva1269I GAATGC 1 cut(s) 633
MvaI CCWGG 1 cut(s) 1036
MwoI GCNNNNNNNGC 5 cut(s) 10, 14, 115, 299, 1031
NaeI GCCGGC 1 cut(s) 15
NciI CCSGG 1 cut(s) 989
NcoI CCATGG 1 cut(s) 719
NdeI CATATG 1 cut(s) 264
NdeII GATC 5 cut(s) 271, 313, 610, 781, 828
NgoMIV GCCGGC 1 cut(s) 13
NheI GCTAGC 1 cut(s) 869
NlaIII CATG 7 cut(s) 104, 430, 478, 593, 681, 723, 978
NlaIV GGNNCC 2 cut(s) 315, 472
NmeAIII GCCGAG 1 cut(s) 787
NmuCI GTSAC 3 cut(s) 30, 885, 901
NspI RCATGY 3 cut(s) 430, 681, 978
PaeI GCATGC 1 cut(s) 978
PcsI WCGNNNNNNNCGW 1 cut(s) 1023
PctI GAATGC 1 cut(s) 633
PdiI GCCGGC 1 cut(s) 15
PdmI GAANNNNTTC 1 cut(s) 802
PfeI GAWTC 4 cut(s) 647, 714, 881, 940
PflMI CCANNNNNTGG 1 cut(s) 323
PleI GAGTC 1 cut(s) 538
PpsI GAGTC 1 cut(s) 538
Psp6I CCWGG 1 cut(s) 1034
PspGI CCWGG 1 cut(s) 1034
PspN4I GGNNCC 2 cut(s) 315, 472
PspPI GGNCC 2 cut(s) 248, 326
PstI CTGCAG 1 cut(s) 898
PstNI CAGNNNCTG 1 cut(s) 156
PsuI RGATCY 1 cut(s) 313
RsaI GTAC 5 cut(s) 154, 160, 567, 744, 854
RsaNI GTAC 5 cut(s) 153, 159, 566, 743, 853
RseI CAYNNNNRTG 1 cut(s) 660
SaqAI TTAA 4 cut(s) 41, 96, 413, 492
Sau3AI GATC 5 cut(s) 271, 313, 610, 781, 828
Sau96I GGNCC 2 cut(s) 248, 326
SchI GAGTC 1 cut(s) 539
ScrFI CCNGG 2 cut(s) 989, 1036
SduI GDGCHC 1 cut(s) 461
SfaNI GCATC 2 cut(s) 352, 427
SfcI CTRYAG 1 cut(s) 894
SfiI GGCCNNNNNGGCC 1 cut(s) 1031
SinI GGWCC 2 cut(s) 248, 326
SmiMI CAYNNNNRTG 1 cut(s) 660
SphI GCATGC 1 cut(s) 978
Sse9I AATT 6 cut(s) 137, 522, 625, 726, 801, 948
SsiI CCGC 3 cut(s) 128, 658, 867
SspI AATATT 1 cut(s) 1014
SspMI CTAG 4 cut(s) 18, 377, 848, 870
StyD4I CCNGG 2 cut(s) 987, 1034
StyI CCWWGG 2 cut(s) 73, 719
TaaI ACNGT 4 cut(s) 63, 152, 693, 1009
TaiI ACGT 2 cut(s) 520, 607
TaqI TCGA 2 cut(s) 135, 520
TasI AATT 6 cut(s) 137, 522, 625, 726, 801, 948
TatI WGTACW 1 cut(s) 158
TfiI GAWTC 4 cut(s) 647, 714, 881, 940
Tru1I TTAA 4 cut(s) 41, 96, 413, 492
Tru9I TTAA 4 cut(s) 41, 96, 413, 492
TscAI CASTG 4 cut(s) 111, 648, 880, 903
TseFI GTSAC 3 cut(s) 30, 885, 901
Tsp45I GTSAC 3 cut(s) 30, 885, 901
TspDTI ATGAA 5 cut(s) 82, 96, 185, 251, 686
TspGWI ACGGA 1 cut(s) 952
TspRI CASTG 4 cut(s) 111, 648, 880, 903
Van91I CCANNNNNTGG 1 cut(s) 323
VpaK11BI GGWCC 2 cut(s) 248, 326
XapI RAATTY 2 cut(s) 137, 522
XbaI TCTAGA 1 cut(s) 847
XceI RCATGY 3 cut(s) 430, 681, 978
XmnI GAANNNNTTC 1 cut(s) 802
XspI CTAG 4 cut(s) 18, 377, 848, 870
ZraI GACGTC 1 cut(s) 518
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.