Rw0G002630

START domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00083
OG0003829
Physical Location & Seq
Reverse (-)
5059 .. 7545
2487 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G002630.1

Sequence Viewer

Length: 1284 bp
ATGGCTTTGGCGTCGGCTTTGCTGGAGATTCTGCAAAGACCCACGATTGTTGAAGTGCTGGGGGAGCTCGTCAAGTTCATAACCCCTCTTTGGATTGCTGTGATTGTCGGGGTTTTCGTTGGGTGGGCGTGGCGGCCCAAATGGGTGACCGTGGTTGGTAGAGAATTGTTGGATAGCTCCACACAAAAGGAGTCATCTTCCTCGTCTCCGCCCGCACCATCCACGACGTGTGGGATTTCGATTTCGAGTTTGAGTTCGTTGCTGCCGAATTGCATTCCTTGGGCTGCTGACGATAGAAATCAGAACCAGGCACCTACCACCAATAATGATTGCAGTTCCTCACAGATTGAACAAGAAGAATCCTGTAATGTCTCCAATGAGGATCTACGCCACATATGTCAGCTTGTGGAGGAGAAAGATGCCGGTCCTGCTTGGATTCATATGATGAATCGGTCAACCCCAACTATGGGATATCAAGCTTGGCGTAGGGATCCTCAGACCGGACCTCCACAATACCGAAGTAGAACTGTATTCGAAGATGCTTCACCTGAGACAGTGAGGGATTTCTTTTGGGATGATGAATTTCGTGCAAAGTGGGATGATATGCTTATTCATGCTTCTACTTTGGAAGAATGCCCGACTACTGGAACCATGCTAGTGCACTGGGTCCGCAAGTTTCCATTCTTTTGTAGCGACAGAGATTACACGATAGGCCGTCGCATTTGGGAATCGGGGCGATCATACTACTGTGTAACTAAGGGAGTTCCTTCCTCTGCTGTGCCAAGGCGTGACAAACCTAGACGTGTTGATCTGTACTATTCAAGTTGGTGCATACGTGCAGTTGAATCAAAAAGAGGGGATGGCCAGCTTACTTCATGTGAGGTTTTACTGTTTCACCATGAAGATATGGGTATTCCATGGGAGATTGCGAAACTTGGTGTTCGTCAAGGTATGTGGGGAGCTGTTAAAAAGATTGAACCAGGCCTACGTTTATATCAAAAGGAAAGAGCCGCTGAAGCACCACTTTCGCGGTGTGCTAAAATGGCTCAGATCAACACGAAAGTCAGTGCTGATTACTTGAGATCCGTAGAAAACAGCGCTAAGGATTCCATTGAGGTTGAAAATTCAGATTCAGCGGAGAAACCAGGAAGGAGGAATATACCTAAAGCTCTAGTTGTCGGAGGGGCAATAGTTCTTGCTTGCAGTCTTGATCGGGGCCTCTTGACTAAGGCAGTTATATTTGGAGTAGCTCGAAGATTTGCCAAGATCGGGAGGAGGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
START

Protein Analysis

427

Amino Acids

47.97

Weight (kDa)

8.39

Isoelectric Point (pI)

55.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
START PF01852 166 - 299 2.3e-08 START domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 310
AccII CGCG 1 cut(s) 1030
AciI CCGC 7 cut(s) 133, 209, 213, 670, 1011, 1030, 1136
AclWI GGATC 4 cut(s) 390, 485, 498, 1077
AcoI YGGCCR 1 cut(s) 862
AcsI RAATTY 2 cut(s) 581, 1123
AcuI CTGAAG 1 cut(s) 1035
AcyI GRCGYC 1 cut(s) 11
AdeI CACNNNGTG 1 cut(s) 228
AfaI GTAC 1 cut(s) 815
AfeI AGCGCT 1 cut(s) 1099
AfiI CCNNNNNNNGG 6 cut(s) 90, 466, 467, 500, 644, 1269
AflIII ACRYGT 2 cut(s) 227, 802
AgsI TTSAA 6 cut(s) 53, 350, 822, 845, 977, 1121
AjiI CACGTC 2 cut(s) 228, 803
AjnI CCWGG 3 cut(s) 306, 979, 1144
AluBI AGCT 8 cut(s) 67, 177, 403, 479, 868, 962, 1169, 1250
AluI AGCT 8 cut(s) 67, 177, 403, 479, 868, 962, 1169, 1250
Alw21I GWGCWC 2 cut(s) 69, 663
Alw26I GTCTC 3 cut(s) 210, 376, 545
Alw44I GTGCAC 1 cut(s) 659
AlwI GGATC 4 cut(s) 390, 485, 498, 1077
Aor51HI AGCGCT 1 cut(s) 1099
AoxI GGCC 5 cut(s) 134, 712, 862, 982, 1216
ApaLI GTGCAC 1 cut(s) 659
ApeKI GCWGC 2 cut(s) 262, 284
ApoI RAATTY 2 cut(s) 581, 1123
AspLEI GCGC 1 cut(s) 1100
AspS9I GGNCC 5 cut(s) 135, 425, 503, 667, 1216
AsuHPI GGTGA 3 cut(s) 157, 537, 887
AsuII TTCGAA 1 cut(s) 534
AvaII GGWCC 3 cut(s) 425, 503, 667
BaeGI GKGCMC 1 cut(s) 663
BalI TGGCCA 1 cut(s) 864
BamHI GGATCC 1 cut(s) 490
BanI GGYRCC 1 cut(s) 310
BanII GRGCYC 1 cut(s) 69
Bbv12I GWGCWC 2 cut(s) 69, 663
BbvI GCAGC 2 cut(s) 249, 271
BccI CCATC 2 cut(s) 226, 854
BceAI ACGGC 1 cut(s) 699
BcgI CGANNNNNNTGC 2 cut(s) 1008, 1042
BciT130I CCWGG 3 cut(s) 308, 981, 1146
BcoDI GTCTC 3 cut(s) 210, 376, 545
BfaI CTAG 3 cut(s) 656, 798, 1172
BfoI RGCGCY 1 cut(s) 1101
BisI GCNGC 4 cut(s) 134, 263, 285, 1011
BlsI GCNGC 4 cut(s) 135, 264, 286, 1012
Bme1390I CCNGG 3 cut(s) 308, 981, 1146
Bme18I GGWCC 3 cut(s) 425, 503, 667
BmgBI CACGTC 2 cut(s) 228, 803
BmgT120I GGNCC 5 cut(s) 135, 425, 503, 667, 1216
BmiI GGNNCC 5 cut(s) 312, 492, 649, 668, 1217
BmrFI CCNGG 3 cut(s) 308, 981, 1146
BmrI ACTGGG 1 cut(s) 673
BmsI GCATC 2 cut(s) 409, 529
BmuI ACTGGG 1 cut(s) 673
BpmI CTGGAG 1 cut(s) 44
Bpu10I CCTNAGC 1 cut(s) 1101
Bpu14I TTCGAA 1 cut(s) 534
BpuEI CTTGAG 1 cut(s) 1099
BsaAI YACGTR 1 cut(s) 836
BsaBI GATNNNNATC 1 cut(s) 297
BsaHI GRCGYC 1 cut(s) 11
BsaJI CCNNGG 4 cut(s) 150, 278, 782, 917
BsaWI WCCGGW 1 cut(s) 500
BsaXI ACNNNNNCTCC 2 cut(s) 490, 520
Bsc4I CCNNNNNNNGG 6 cut(s) 90, 466, 467, 500, 644, 1269
Bse118I RCCGGY 1 cut(s) 422
Bse1I ACTGG 2 cut(s) 649, 668
Bse8I GATNNNNATC 1 cut(s) 297
BseBI CCWGG 3 cut(s) 308, 981, 1146
BseDI CCNNGG 4 cut(s) 150, 278, 782, 917
BseGI GGATG 4 cut(s) 218, 580, 604, 865
BseJI GATNNNNATC 1 cut(s) 297
BseLI CCNNNNNNNGG 6 cut(s) 90, 466, 467, 500, 644, 1269
BseMII CTCAG 3 cut(s) 509, 540, 1061
BseNI ACTGG 2 cut(s) 649, 668
BseRI GAGGAG 1 cut(s) 425
BseSI GKGCMC 1 cut(s) 663
BseXI GCAGC 2 cut(s) 249, 271
BseYI CCCAGC 1 cut(s) 58
BsgI GTGCAG 1 cut(s) 858
Bsh1236I CGCG 1 cut(s) 1030
BshFI GGCC 5 cut(s) 136, 714, 864, 984, 1218
BshNI GGYRCC 1 cut(s) 310
BsiHKAI GWGCWC 2 cut(s) 69, 663
BsiSI CCGG 2 cut(s) 423, 501
BslI CCNNNNNNNGG 6 cut(s) 90, 466, 467, 500, 644, 1269
BsmAI GTCTC 3 cut(s) 210, 376, 545
BsmBI CGTCTC 1 cut(s) 210
BsmI GAATGC 2 cut(s) 273, 638
BsnI GGCC 5 cut(s) 136, 714, 864, 984, 1218
Bsp119I TTCGAA 1 cut(s) 534
Bsp1286I GDGCHC 2 cut(s) 69, 663
Bsp143I GATC 8 cut(s) 382, 490, 737, 808, 1050, 1082, 1210, 1266
Bsp19I CCATGG 1 cut(s) 917
BspACI CCGC 7 cut(s) 133, 209, 213, 670, 1011, 1030, 1136
BspANI GGCC 5 cut(s) 136, 714, 864, 984, 1218
BspCNI CTCAG 3 cut(s) 508, 541, 1060
BspFNI CGCG 1 cut(s) 1030
BspLI GGNNCC 5 cut(s) 312, 492, 649, 668, 1217
BspPI GGATC 4 cut(s) 390, 485, 498, 1077
BspT104I TTCGAA 1 cut(s) 534
BspT107I GGYRCC 1 cut(s) 310
BsrFI RCCGGY 1 cut(s) 422
BsrI ACTGG 2 cut(s) 649, 668
BssAI RCCGGY 1 cut(s) 422
BssECI CCNNGG 4 cut(s) 150, 278, 782, 917
BssMI GATC 8 cut(s) 382, 490, 737, 808, 1050, 1082, 1210, 1266
BssNI GRCGYC 1 cut(s) 11
BssT1I CCWWGG 3 cut(s) 278, 782, 917
Bst2UI CCWGG 3 cut(s) 308, 981, 1146
Bst4CI ACNGT 5 cut(s) 151, 529, 556, 749, 891
BstACI GRCGYC 1 cut(s) 11
BstBAI YACGTR 1 cut(s) 836
BstBI TTCGAA 1 cut(s) 534
BstC8I GCNNGC 3 cut(s) 213, 866, 1201
BstDEI CTNAG 6 cut(s) 495, 549, 756, 1047, 1101, 1227
BstDSI CCRYGG 2 cut(s) 150, 917
BstEII GGTNACC 1 cut(s) 145
BstF5I GGATG 4 cut(s) 218, 580, 604, 865
BstFNI CGCG 1 cut(s) 1030
BstH2I RGCGCY 1 cut(s) 1101
BstHHI GCGC 1 cut(s) 1100
BstKTI GATC 8 cut(s) 385, 493, 740, 811, 1053, 1085, 1213, 1269
BstMAI GTCTC 3 cut(s) 210, 376, 545
BstMBI GATC 8 cut(s) 382, 490, 737, 808, 1050, 1082, 1210, 1266
BstMWI GCNNNNNNNGC 4 cut(s) 64, 428, 1016, 1043
BstNI CCWGG 3 cut(s) 308, 981, 1146
BstPI GGTNACC 1 cut(s) 145
BstSCI CCNGG 3 cut(s) 306, 979, 1144
BstSLI GKGCMC 1 cut(s) 663
BstUI CGCG 1 cut(s) 1030
BstV1I GCAGC 2 cut(s) 249, 271
BstX2I RGATCY 3 cut(s) 382, 490, 1082
BstYI RGATCY 3 cut(s) 382, 490, 1082
BsuRI GGCC 5 cut(s) 136, 714, 864, 984, 1218
BtgI CCRYGG 2 cut(s) 150, 917
BtrI CACGTC 2 cut(s) 228, 803
BtsCI GGATG 4 cut(s) 218, 580, 604, 865
BtsIMutI CAGTG 3 cut(s) 561, 661, 1072
Cac8I GCNNGC 3 cut(s) 213, 866, 1201
CfoI GCGC 1 cut(s) 1100
Cfr10I RCCGGY 1 cut(s) 422
Cfr13I GGNCC 5 cut(s) 135, 425, 503, 667, 1216
Csp6I GTAC 1 cut(s) 814
CspCI CAANNNNNGTGG 4 cut(s) 31, 66, 935, 970
CviAII CATG 5 cut(s) 614, 652, 876, 899, 918
CviQI GTAC 1 cut(s) 814
DdeI CTNAG 6 cut(s) 495, 549, 756, 1047, 1101, 1227
DpnI GATC 8 cut(s) 384, 492, 739, 810, 1052, 1084, 1212, 1268
DpnII GATC 8 cut(s) 382, 490, 737, 808, 1050, 1082, 1210, 1266
DraIII CACNNNGTG 1 cut(s) 228
EaeI YGGCCR 1 cut(s) 862
EciI GGCGGA 1 cut(s) 198
Ecl136II GAGCTC 1 cut(s) 67
Eco130I CCWWGG 3 cut(s) 278, 782, 917
Eco147I AGGCCT 1 cut(s) 984
Eco24I GRGCYC 1 cut(s) 69
Eco32I GATATC 1 cut(s) 473
Eco47I GGWCC 3 cut(s) 425, 503, 667
Eco47III AGCGCT 1 cut(s) 1099
Eco53kI GAGCTC 1 cut(s) 67
Eco57I CTGAAG 1 cut(s) 1035
Eco91I GGTNACC 1 cut(s) 145
EcoICRI GAGCTC 1 cut(s) 67
EcoO109I RGGNCCY 1 cut(s) 1216
EcoO65I GGTNACC 1 cut(s) 145
EcoRII CCWGG 3 cut(s) 306, 979, 1144
EcoRV GATATC 1 cut(s) 473
EcoT14I CCWWGG 3 cut(s) 278, 782, 917
EcoT38I GRGCYC 1 cut(s) 69
ErhI CCWWGG 3 cut(s) 278, 782, 917
Esp3I CGTCTC 1 cut(s) 210
FaeI CATG 5 cut(s) 617, 655, 879, 902, 921
FalI AAGNNNNNCTT 2 cut(s) 1008, 1040
FatI CATG 5 cut(s) 613, 651, 875, 898, 917
FauI CCCGC 1 cut(s) 220
FauNDI CATATG 2 cut(s) 395, 441
Fnu4HI GCNGC 4 cut(s) 134, 263, 285, 1011
FokI GGATG 4 cut(s) 205, 587, 611, 872
FriOI GRGCYC 1 cut(s) 69
Fsp4HI GCNGC 4 cut(s) 134, 263, 285, 1011
FspBI CTAG 3 cut(s) 656, 798, 1172
GlaI GCGC 1 cut(s) 1099
GluI GCNGC 4 cut(s) 134, 263, 285, 1011
GsaI CCCAGC 1 cut(s) 62
GsuI CTGGAG 1 cut(s) 44
HaeII RGCGCY 1 cut(s) 1101
HaeIII GGCC 5 cut(s) 136, 714, 864, 984, 1218
HapII CCGG 2 cut(s) 423, 501
HhaI GCGC 1 cut(s) 1100
Hin1I GRCGYC 1 cut(s) 11
Hin1II CATG 5 cut(s) 617, 655, 879, 902, 921
Hin6I GCGC 1 cut(s) 1098
HinP1I GCGC 1 cut(s) 1098
HincII GTYRAC 1 cut(s) 456
HindII GTYRAC 1 cut(s) 456
HindIII AAGCTT 1 cut(s) 477
HinfI GANTC 9 cut(s) 28, 191, 359, 436, 448, 728, 845, 1106, 1130
HpaII CCGG 2 cut(s) 423, 501
HphI GGTGA 3 cut(s) 157, 537, 887
Hpy166II GTNNAC 2 cut(s) 456, 661
Hpy188I TCNGA 5 cut(s) 303, 498, 1050, 1129, 1181
Hpy188III TCNNGA 3 cut(s) 1208, 1222, 1270
Hpy8I GTNNAC 2 cut(s) 456, 661
Hpy99I CGWCG 3 cut(s) 16, 229, 720
HpyAV CCTTC 2 cut(s) 777, 1143
HpyCH4III ACNGT 5 cut(s) 151, 529, 556, 749, 891
HpyCH4IV ACGT 4 cut(s) 227, 802, 835, 988
HpyCH4V TGCA 8 cut(s) 34, 273, 333, 590, 661, 831, 839, 1203
HpyF10VI GCNNNNNNNGC 4 cut(s) 64, 428, 1016, 1043
HpyF3I CTNAG 6 cut(s) 495, 549, 756, 1047, 1101, 1227
HpySE526I ACGT 4 cut(s) 227, 802, 835, 988
Hsp92I GRCGYC 1 cut(s) 11
Hsp92II CATG 5 cut(s) 617, 655, 879, 902, 921
HspAI GCGC 1 cut(s) 1098
Kzo9I GATC 8 cut(s) 382, 490, 737, 808, 1050, 1082, 1210, 1266
LmnI GCTCC 3 cut(s) 64, 182, 959
Lsp1109I GCAGC 2 cut(s) 249, 271
LweI GCATC 2 cut(s) 409, 529
MaeI CTAG 3 cut(s) 656, 798, 1172
MaeII ACGT 4 cut(s) 227, 802, 835, 988
MaeIII GTNAC 3 cut(s) 145, 751, 788
MalI GATC 8 cut(s) 384, 492, 739, 810, 1052, 1084, 1212, 1268
MboI GATC 8 cut(s) 382, 490, 737, 808, 1050, 1082, 1210, 1266
MboII GAAGA 6 cut(s) 189, 368, 548, 641, 914, 1266
MflI RGATCY 3 cut(s) 382, 490, 1082
MhlI GDGCHC 2 cut(s) 69, 663
MlsI TGGCCA 1 cut(s) 864
MluCI AATT 4 cut(s) 164, 268, 581, 1123
MluNI TGGCCA 1 cut(s) 864
MlyI GAGTC 1 cut(s) 200
MmeI TCCRAC 2 cut(s) 150, 1159
Mox20I TGGCCA 1 cut(s) 864
MscI TGGCCA 1 cut(s) 864
MseI TTAA 1 cut(s) 966
MslI CAYNNNNRTG 1 cut(s) 656
Msp20I TGGCCA 1 cut(s) 864
MspA1I CMGCKG 2 cut(s) 1013, 1136
MspI CCGG 2 cut(s) 423, 501
MspR9I CCNGG 3 cut(s) 308, 981, 1146
Mva1269I GAATGC 2 cut(s) 273, 638
MvaI CCWGG 3 cut(s) 308, 981, 1146
MvnI CGCG 1 cut(s) 1030
MwoI GCNNNNNNNGC 4 cut(s) 64, 428, 1016, 1043
NcoI CCATGG 1 cut(s) 917
NdeI CATATG 2 cut(s) 395, 441
NdeII GATC 8 cut(s) 382, 490, 737, 808, 1050, 1082, 1210, 1266
NlaIII CATG 5 cut(s) 617, 655, 879, 902, 921
NlaIV GGNNCC 5 cut(s) 312, 492, 649, 668, 1217
NmuCI GTSAC 2 cut(s) 145, 788
NspV TTCGAA 1 cut(s) 534
PceI AGGCCT 1 cut(s) 984
PcsI WCGNNNNNNNCGW 2 cut(s) 114, 263
PctI GAATGC 2 cut(s) 273, 638
PfeI GAWTC 8 cut(s) 28, 359, 436, 448, 728, 845, 1106, 1130
PkrI GCNGC 4 cut(s) 135, 264, 286, 1012
PleI GAGTC 1 cut(s) 199
PpsI GAGTC 1 cut(s) 199
Ppu21I YACGTR 1 cut(s) 836
Psp124BI GAGCTC 1 cut(s) 69
Psp6I CCWGG 3 cut(s) 306, 979, 1144
PspEI GGTNACC 1 cut(s) 145
PspFI CCCAGC 1 cut(s) 58
PspGI CCWGG 3 cut(s) 306, 979, 1144
PspN4I GGNNCC 5 cut(s) 312, 492, 649, 668, 1217
PspPI GGNCC 5 cut(s) 135, 425, 503, 667, 1216
PsrI GAACNNNNNNTAC 2 cut(s) 969, 1001
PsuI RGATCY 3 cut(s) 382, 490, 1082
RsaI GTAC 1 cut(s) 815
RsaNI GTAC 1 cut(s) 814
RseI CAYNNNNRTG 1 cut(s) 656
SacI GAGCTC 1 cut(s) 69
SaqAI TTAA 1 cut(s) 966
SatI GCNGC 4 cut(s) 134, 263, 285, 1011
Sau3AI GATC 8 cut(s) 382, 490, 737, 808, 1050, 1082, 1210, 1266
Sau96I GGNCC 5 cut(s) 135, 425, 503, 667, 1216
SchI GAGTC 1 cut(s) 200
ScrFI CCNGG 3 cut(s) 308, 981, 1146
SduI GDGCHC 2 cut(s) 69, 663
SfaNI GCATC 2 cut(s) 409, 529
SfuI TTCGAA 1 cut(s) 534
SinI GGWCC 3 cut(s) 425, 503, 667
SmiMI CAYNNNNRTG 1 cut(s) 656
SmlI CTYRAG 1 cut(s) 1078
SmoI CTYRAG 1 cut(s) 1078
Sse9I AATT 4 cut(s) 164, 268, 581, 1123
SseBI AGGCCT 1 cut(s) 984
SsiI CCGC 7 cut(s) 133, 209, 213, 670, 1011, 1030, 1136
SspMI CTAG 3 cut(s) 656, 798, 1172
SstI GAGCTC 1 cut(s) 69
StuI AGGCCT 1 cut(s) 984
StyD4I CCNGG 3 cut(s) 306, 979, 1144
StyI CCWWGG 3 cut(s) 278, 782, 917
TaaI ACNGT 5 cut(s) 151, 529, 556, 749, 891
TaiI ACGT 4 cut(s) 230, 805, 838, 991
TaqI TCGA 4 cut(s) 239, 245, 534, 1252
TaqII GACCGA 1 cut(s) 441
TasI AATT 4 cut(s) 164, 268, 581, 1123
TatI WGTACW 1 cut(s) 813
TauI GCSGC 2 cut(s) 136, 1013
TfiI GAWTC 8 cut(s) 28, 359, 436, 448, 728, 845, 1106, 1130
Tru1I TTAA 1 cut(s) 966
Tru9I TTAA 1 cut(s) 966
TscAI CASTG 3 cut(s) 561, 668, 1072
TseFI GTSAC 2 cut(s) 145, 788
TseI GCWGC 2 cut(s) 262, 284
Tsp45I GTSAC 2 cut(s) 145, 788
TspDTI ATGAA 7 cut(s) 67, 428, 461, 594, 602, 864, 915
TspGWI ACGGA 1 cut(s) 1075
TspRI CASTG 3 cut(s) 561, 668, 1072
VneI GTGCAC 1 cut(s) 659
VpaK11BI GGWCC 3 cut(s) 425, 503, 667
XapI RAATTY 2 cut(s) 581, 1123
XspI CTAG 3 cut(s) 656, 798, 1172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.