MD14G1184500.v1.1

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0016660
Physical Location & Seq
Reverse (-)
27678975 .. 27681454
2480 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1184500.v1.1.491

Sequence Viewer

Length: 261 bp
ATGGAGAAGATGAGTGAGGTGGATCCCACGTTAGAAGTATTTTACGAGGAGAAGAAGCGGGTCCGCAACCCTTGGGTACCAGTTGGTGCGATGTTGACTGCTGGTGTGCTCACCGCCGGCTTAATCAGTTTCAGGCAAGGCAATTCTCAGTTGGGCCAGAAGCTAATGAGAGCTCGTGTCGTTGTCCAAGGTGCCACAGTGGCTCTTATGGTTGGCAGTGCATACTACTACGGGGATAATCCGTGGAAAAAGCCAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HIG_1_N

Protein Analysis

87

Amino Acids

9.55

Weight (kDa)

9.63

Isoelectric Point (pI)

34.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HIG_1_N PF04588 23 - 72 1.8e-20 Hypoxia induced protein conserved region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016660)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G48030 AT3G48030
fragaria_vesca FvH4_5g05020
malus_domestica MD06G1177900.v1.1 MD14G1184500.v1.1
prunus_persica Prupe.5G177100_v2.0.a1
pyrus_communis pycom14g15350
rosa_chinensis RchiOBHm_Chr7g0194021
rosa_roxburghii Rroxscaffold_3G00261610
rosa_rugosa Rorug07G0013200
rosa_samantha Rh7AG140500 Rh7BG141000 Rh7DG141900
rosa_wichuraiana Rw7G011990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 76
AccB1I GGYRCC 2 cut(s) 76, 191
AciI CCGC 3 cut(s) 58, 64, 114
AclWI GGATC 2 cut(s) 17, 30
AfaI GTAC 1 cut(s) 78
AluBI AGCT 2 cut(s) 163, 173
AluI AGCT 2 cut(s) 163, 173
Alw21I GWGCWC 2 cut(s) 111, 175
AlwI GGATC 2 cut(s) 17, 30
AoxI GGCC 1 cut(s) 154
Asp718I GGTACC 1 cut(s) 76
AspS9I GGNCC 2 cut(s) 61, 154
AsuHPI GGTGA 1 cut(s) 103
AvaII GGWCC 1 cut(s) 61
BamHI GGATCC 1 cut(s) 22
BanI GGYRCC 2 cut(s) 76, 191
BanII GRGCYC 1 cut(s) 175
BauI CACGAG 1 cut(s) 174
Bbv12I GWGCWC 2 cut(s) 111, 175
BglI GCCNNNNNGGC 1 cut(s) 200
Bme18I GGWCC 1 cut(s) 61
BmgT120I GGNCC 2 cut(s) 61, 154
BmiI GGNNCC 4 cut(s) 24, 62, 78, 193
BsaJI CCNNGG 3 cut(s) 71, 187, 242
Bse118I RCCGGY 1 cut(s) 116
Bse1I ACTGG 1 cut(s) 80
BseDI CCNNGG 3 cut(s) 71, 187, 242
BseMII CTCAG 1 cut(s) 161
BseNI ACTGG 1 cut(s) 80
BseRI GAGGAG 1 cut(s) 62
BshFI GGCC 1 cut(s) 156
BshNI GGYRCC 2 cut(s) 76, 191
BsiHKAI GWGCWC 2 cut(s) 111, 175
BsiSI CCGG 1 cut(s) 117
BsnI GGCC 1 cut(s) 156
Bsp1286I GDGCHC 2 cut(s) 111, 175
Bsp143I GATC 1 cut(s) 22
BspACI CCGC 3 cut(s) 58, 64, 114
BspANI GGCC 1 cut(s) 156
BspCNI CTCAG 1 cut(s) 160
BspLI GGNNCC 4 cut(s) 24, 62, 78, 193
BspPI GGATC 2 cut(s) 17, 30
BspT107I GGYRCC 2 cut(s) 76, 191
BsrFI RCCGGY 1 cut(s) 116
BsrI ACTGG 1 cut(s) 80
BssAI RCCGGY 1 cut(s) 116
BssECI CCNNGG 3 cut(s) 71, 187, 242
BssMI GATC 1 cut(s) 22
BssSI CACGAG 1 cut(s) 174
BssT1I CCWWGG 2 cut(s) 71, 187
Bst2BI CACGAG 1 cut(s) 174
Bst4CI ACNGT 1 cut(s) 199
BstC8I GCNNGC 1 cut(s) 118
BstDEI CTNAG 1 cut(s) 147
BstDSI CCRYGG 1 cut(s) 242
BstKTI GATC 1 cut(s) 25
BstMBI GATC 1 cut(s) 22
BstMWI GCNNNNNNNGC 1 cut(s) 200
BstX2I RGATCY 1 cut(s) 22
BstYI RGATCY 1 cut(s) 22
BsuRI GGCC 1 cut(s) 156
BtgI CCRYGG 1 cut(s) 242
BtgZI GCGATG 1 cut(s) 104
BtsI GCAGTG 1 cut(s) 223
BtsIMutI CAGTG 2 cut(s) 204, 223
Cac8I GCNNGC 1 cut(s) 118
Cfr10I RCCGGY 1 cut(s) 116
Cfr13I GGNCC 2 cut(s) 61, 154
Csp6I GTAC 1 cut(s) 77
CviJI RGCY 6 cut(s) 120, 156, 163, 173, 203, 253
CviKI_1 RGCY 6 cut(s) 120, 156, 163, 173, 203, 253
CviQI GTAC 1 cut(s) 77
DdeI CTNAG 1 cut(s) 147
DpnI GATC 1 cut(s) 24
DpnII GATC 1 cut(s) 22
Ecl136II GAGCTC 1 cut(s) 173
Eco130I CCWWGG 2 cut(s) 71, 187
Eco24I GRGCYC 1 cut(s) 175
Eco47I GGWCC 1 cut(s) 61
Eco53kI GAGCTC 1 cut(s) 173
EcoICRI GAGCTC 1 cut(s) 173
EcoT14I CCWWGG 2 cut(s) 71, 187
EcoT38I GRGCYC 1 cut(s) 175
ErhI CCWWGG 2 cut(s) 71, 187
FaiI YATR 2 cut(s) 209, 223
FauI CCCGC 1 cut(s) 51
FriOI GRGCYC 1 cut(s) 175
HaeIII GGCC 1 cut(s) 156
HapII CCGG 1 cut(s) 117
HincII GTYRAC 1 cut(s) 96
HindII GTYRAC 1 cut(s) 96
HpaII CCGG 1 cut(s) 117
HphI GGTGA 1 cut(s) 103
Hpy166II GTNNAC 1 cut(s) 96
Hpy8I GTNNAC 1 cut(s) 96
HpyCH4III ACNGT 1 cut(s) 199
HpyCH4IV ACGT 1 cut(s) 29
HpyCH4V TGCA 1 cut(s) 221
HpyF10VI GCNNNNNNNGC 1 cut(s) 200
HpyF3I CTNAG 1 cut(s) 147
HpySE526I ACGT 1 cut(s) 29
KpnI GGTACC 1 cut(s) 80
KroI GCCGGC 1 cut(s) 116
KroNI GCCGGC 1 cut(s) 118
Kzo9I GATC 1 cut(s) 22
LpnPI CCDG 5 cut(s) 87, 93, 118, 130, 170
MaeII ACGT 1 cut(s) 29
MalI GATC 1 cut(s) 24
MboI GATC 1 cut(s) 22
MboII GAAGA 2 cut(s) 19, 64
MflI RGATCY 1 cut(s) 22
MhlI GDGCHC 2 cut(s) 111, 175
MluCI AATT 1 cut(s) 142
MnlI CCTC 2 cut(s) 10, 40
MroNI GCCGGC 1 cut(s) 116
MseI TTAA 2 cut(s) 122, 259
MspI CCGG 1 cut(s) 117
MwoI GCNNNNNNNGC 1 cut(s) 200
NaeI GCCGGC 1 cut(s) 118
NdeII GATC 1 cut(s) 22
NgoMIV GCCGGC 1 cut(s) 116
NlaIV GGNNCC 4 cut(s) 24, 62, 78, 193
PdiI GCCGGC 1 cut(s) 118
Psp124BI GAGCTC 1 cut(s) 175
PspN4I GGNNCC 4 cut(s) 24, 62, 78, 193
PspPI GGNCC 2 cut(s) 61, 154
PsuI RGATCY 1 cut(s) 22
RsaI GTAC 1 cut(s) 78
RsaNI GTAC 1 cut(s) 77
SacI GAGCTC 1 cut(s) 175
SaqAI TTAA 2 cut(s) 122, 259
Sau3AI GATC 1 cut(s) 22
Sau96I GGNCC 2 cut(s) 61, 154
SduI GDGCHC 2 cut(s) 111, 175
SetI ASST 5 cut(s) 21, 32, 165, 175, 193
SinI GGWCC 1 cut(s) 61
Sse9I AATT 1 cut(s) 142
SsiI CCGC 3 cut(s) 58, 64, 114
SstI GAGCTC 1 cut(s) 175
StyI CCWWGG 2 cut(s) 71, 187
TaaI ACNGT 1 cut(s) 199
TaiI ACGT 1 cut(s) 32
TasI AATT 1 cut(s) 142
Tru1I TTAA 2 cut(s) 122, 259
Tru9I TTAA 2 cut(s) 122, 259
TscAI CASTG 2 cut(s) 204, 223
TspGWI ACGGA 1 cut(s) 231
TspRI CASTG 2 cut(s) 204, 223
VpaK11BI GGWCC 1 cut(s) 61
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.