MD14G1184700.v1.1
ERF Family

Belongs to the small GTPase superfamily. Rho family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0013804
Physical Location & Seq
Forward (+)
27711640 .. 27714763
3124 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1184700.v1.1.491

Sequence Viewer

Length: 636 bp
ATGGCTTCAAGCGCTTCAAGGTTCATCAAGTGTGTGACTGTGGGAGATGGGGCTGTGGGGAAGACCTGTATGCTTATTTGCTACACCAGCAACAAGTTTCCCACTGACTATATACCAACTGTGTTTGACAACTTCAGTGCAAATGTGGTGGTTGAAGGCACCACTGTGAACTTAGGCTTATGGGACACTGCTGGGCAAGAGGATTACAATCGGTTGAGGCCATTGAGCTACAGAGGGGCAGATGTCTTTGTCTTGGCTTTCTCATTAGTTAGCCGTGCGAGCTATGAGAATGTATTGAAAAAGTGGATCCCTGAACTCCAGCATTATGCTCCCGGAGTGCCGGTAGTACTTGCCGGCACGAAATTAGATCTTCGAGAGGATAAGCACTATTTGGCTGATCACCCGGGGTTGGTACCTGTGACCACTGCACAGGGTGAAGAGCTGCGCAAACAGATTGGTGCTTCTTTTTATATAGAATGCAGCTCAAAAACCCAACAGAATGTGAAAGCAGTTTTCGATGCTGCGATCAGGGTGGTTATCAAGCCGCCGCAGAAGCAGAAGGAAAAGAAGAAAAAGCAACCTCGGGGATGCCTAGTCAACATCCTTTGCGGGAGGAGGCTAGTGTGCCTCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003824 GO:0003924 GO:0005488 GO:0005515 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005856 GO:0005886 GO:0007154 GO:0007165 GO:0008150 GO:0009505 GO:0009531 GO:0009664 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009787 GO:0009788 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010646 GO:0010648 GO:0016020 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017111 GO:0019899 GO:0019900 GO:0019901 GO:0019902 GO:0019903 GO:0023051 GO:0023052 GO:0023057 GO:0030312 GO:0032870 GO:0033993 GO:0042221 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044424 GO:0044464 GO:0045229 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071554 GO:0071555 GO:0071669 GO:0071840 GO:0071944 GO:0097305 GO:0097306 GO:1901419 GO:1901420 GO:1901700 GO:1901701 GO:1905957 GO:1905958
KEGG Pathways
Metabolic & Signaling
ko04010 ko04013 ko04014 ko04015 ko04024 ko04062 ko04071 ko04145 ko04151 ko04310 ko04360 ko04370 ko04380 ko04510 ko04520 ko04530 ko04620 ko04650 ko04662 ko04664 ko04666 ko04670 ko04722 ko04810 ko04932 ko04933 ko04972 ko05014 ko05100 ko05120 ko05131 ko05132 ko05167 ko05200 ko05203 ko05205 ko05210 ko05211 ko05212 ko05231 ko05416 ko05418 map04010 map04013 map04014 map04015 map04024 map04062 map04071 map04145 map04151 map04310 map04360 map04370 map04380 map04510 map04520 map04530 map04620 map04650 map04662 map04664 map04666 map04670 map04722 map04810 map04932 map04933 map04972 map05014 map05100 map05120 map05131 map05132 map05167 map05200 map05203 map05205 map05210 map05211 map05212 map05231 map05416 map05418
Pfam Domains
Protein Families
Ras

Protein Analysis

212

Amino Acids

23.38

Weight (kDa)

9.3

Isoelectric Point (pI)

47.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ras PF00071 10 - 179 1.6e-48 Ras family
Roc PF08477 10 - 123 4e-22 Ras of Complex, Roc, domain of DAPkinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013804)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G48040 AT3G48040 AT5G62880 AT5G62880
fragaria_vesca FvH4_5g16650 FvH4_5g16650
malus_domestica MD14G1184700.v1.1
prunus_persica Prupe.5G177400_v2.0.a1 Prupe.5G177400_v2.0.a1
pyrus_communis pycom14g15370
rosa_chinensis RchiOBHm_Chr7g0179501
rosa_laevigata RLG00000005349
rosa_multiflora Rmu_sc0000735.1_g000009
rosa_roxburghii Rroxscaffold_3G00273940
rosa_rugosa Rorug06G0424900
rosa_samantha Rh7CG025400
rosa_wichuraiana Rw7G001990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 446
Acc65I GGTACC 1 cut(s) 412
AccB1I GGYRCC 2 cut(s) 158, 412
AciI CCGC 3 cut(s) 545, 548, 609
AclWI GGATC 2 cut(s) 301, 314
AcuI CTGAAG 1 cut(s) 118
AdeI CACNNNGTG 1 cut(s) 434
AfaI GTAC 2 cut(s) 348, 414
AfeI AGCGCT 1 cut(s) 13
AfiI CCNNNNNNNGG 1 cut(s) 409
AgsI TTSAA 4 cut(s) 9, 18, 155, 298
AleI CACNNNNGTG 1 cut(s) 164
AluBI AGCT 4 cut(s) 228, 282, 442, 483
AluI AGCT 4 cut(s) 228, 282, 442, 483
AlwI GGATC 2 cut(s) 301, 314
Ama87I CYCGRG 2 cut(s) 403, 582
Aor51HI AGCGCT 1 cut(s) 13
AoxI GGCC 1 cut(s) 218
ApeKI GCWGC 3 cut(s) 442, 480, 521
Asp718I GGTACC 1 cut(s) 412
AspLEI GCGC 2 cut(s) 14, 447
AsuC2I CCSGG 3 cut(s) 333, 404, 405
AsuHPI GGTGA 2 cut(s) 392, 446
AvaI CYCGRG 2 cut(s) 403, 582
BamHI GGATCC 1 cut(s) 306
BanI GGYRCC 2 cut(s) 158, 412
BbsI GAAGAC 1 cut(s) 68
BbvI GCAGC 3 cut(s) 429, 492, 508
BccI CCATC 1 cut(s) 41
BceAI ACGGC 1 cut(s) 258
BclI TGATCA 1 cut(s) 397
BcnI CCSGG 3 cut(s) 333, 404, 405
BfaI CTAG 2 cut(s) 593, 620
BfmI CTRYAG 1 cut(s) 229
BfoI RGCGCY 1 cut(s) 15
BglII AGATCT 1 cut(s) 367
BisI GCNGC 5 cut(s) 443, 481, 522, 545, 548
BlsI GCNGC 5 cut(s) 444, 482, 523, 546, 549
BmcAI AGTACT 1 cut(s) 348
Bme1390I CCNGG 3 cut(s) 333, 404, 405
BmeT110I CYCGRG 2 cut(s) 403, 582
BmiI GGNNCC 3 cut(s) 160, 308, 414
BmrFI CCNGG 3 cut(s) 333, 404, 405
BmsI GCATC 2 cut(s) 508, 578
BpiI GAAGAC 1 cut(s) 68
BpmI CTGGAG 1 cut(s) 302
BpuEI CTTGAG 1 cut(s) 614
BpuMI CCSGG 3 cut(s) 333, 404, 405
BsaBI GATNNNNATC 1 cut(s) 207
BsaJI CCNNGG 3 cut(s) 403, 404, 581
BsaXI ACNNNNNCTCC 2 cut(s) 327, 357
Bsc4I CCNNNNNNNGG 1 cut(s) 409
Bse118I RCCGGY 2 cut(s) 340, 353
Bse8I GATNNNNATC 1 cut(s) 207
BseDI CCNNGG 3 cut(s) 403, 404, 581
BseGI GGATG 2 cut(s) 593, 600
BseJI GATNNNNATC 1 cut(s) 207
BseLI CCNNNNNNNGG 1 cut(s) 409
BseRI GAGGAG 1 cut(s) 628
BseXI GCAGC 3 cut(s) 429, 492, 508
BseYI CCCAGC 1 cut(s) 191
BsgI GTGCAG 1 cut(s) 411
BshFI GGCC 1 cut(s) 220
BshNI GGYRCC 2 cut(s) 158, 412
BsiHKCI CYCGRG 2 cut(s) 403, 582
BsiSI CCGG 4 cut(s) 333, 341, 354, 404
BslFI GGGAC 1 cut(s) 197
BslI CCNNNNNNNGG 1 cut(s) 409
BsmFI GGGAC 1 cut(s) 197
BsmI GAATGC 1 cut(s) 482
BsnI GGCC 1 cut(s) 220
BsoBI CYCGRG 2 cut(s) 403, 582
Bsp143I GATC 4 cut(s) 306, 367, 397, 525
BspACI CCGC 3 cut(s) 545, 548, 609
BspANI GGCC 1 cut(s) 220
BspLI GGNNCC 3 cut(s) 160, 308, 414
BspPI GGATC 2 cut(s) 301, 314
BspQI GCTCTTC 1 cut(s) 432
BspT107I GGYRCC 2 cut(s) 158, 412
BsrFI RCCGGY 2 cut(s) 340, 353
BssAI RCCGGY 2 cut(s) 340, 353
BssECI CCNNGG 3 cut(s) 403, 404, 581
BssMI GATC 4 cut(s) 306, 367, 397, 525
Bst4CI ACNGT 3 cut(s) 40, 121, 166
Bst6I CTCTTC 1 cut(s) 432
BstC8I GCNNGC 2 cut(s) 280, 355
BstDEI CTNAG 1 cut(s) 172
BstF5I GGATG 2 cut(s) 593, 600
BstH2I RGCGCY 1 cut(s) 15
BstHHI GCGC 2 cut(s) 14, 447
BstKTI GATC 4 cut(s) 309, 370, 400, 528
BstMBI GATC 4 cut(s) 306, 367, 397, 525
BstMWI GCNNNNNNNGC 4 cut(s) 11, 87, 279, 553
BstSCI CCNGG 3 cut(s) 331, 402, 403
BstSFI CTRYAG 1 cut(s) 229
BstV1I GCAGC 3 cut(s) 429, 492, 508
BstV2I GAAGAC 1 cut(s) 68
BstX2I RGATCY 2 cut(s) 306, 367
BstYI RGATCY 2 cut(s) 306, 367
BsuRI GGCC 1 cut(s) 220
BtsCI GGATG 2 cut(s) 593, 600
BtsI GCAGTG 2 cut(s) 186, 423
BtsIMutI CAGTG 5 cut(s) 102, 142, 162, 186, 423
Cac8I GCNNGC 2 cut(s) 280, 355
CfoI GCGC 2 cut(s) 14, 447
Cfr10I RCCGGY 2 cut(s) 340, 353
Cfr9I CCCGGG 1 cut(s) 403
Csp6I GTAC 2 cut(s) 347, 413
CspCI CAANNNNNGTGG 2 cut(s) 129, 164
CviQI GTAC 2 cut(s) 347, 413
DdeI CTNAG 1 cut(s) 172
DpnI GATC 4 cut(s) 308, 369, 399, 527
DpnII GATC 4 cut(s) 306, 367, 397, 525
DraIII CACNNNGTG 1 cut(s) 434
Eam1104I CTCTTC 1 cut(s) 432
EarI CTCTTC 1 cut(s) 432
Eco47III AGCGCT 1 cut(s) 13
Eco57I CTGAAG 1 cut(s) 118
Eco88I CYCGRG 2 cut(s) 403, 582
FaiI YATR 8 cut(s) 71, 111, 113, 181, 285, 327, 471, 473
FaqI GGGAC 1 cut(s) 197
FauI CCCGC 1 cut(s) 602
FbaI TGATCA 1 cut(s) 397
Fnu4HI GCNGC 5 cut(s) 443, 481, 522, 545, 548
FokI GGATG 2 cut(s) 587, 600
Fsp4HI GCNGC 5 cut(s) 443, 481, 522, 545, 548
FspBI CTAG 2 cut(s) 593, 620
FspI TGCGCA 1 cut(s) 446
GlaI GCGC 2 cut(s) 13, 446
GluI GCNGC 5 cut(s) 443, 481, 522, 545, 548
GsaI CCCAGC 1 cut(s) 195
GsuI CTGGAG 1 cut(s) 302
HaeII RGCGCY 1 cut(s) 15
HaeIII GGCC 1 cut(s) 220
HapII CCGG 4 cut(s) 333, 341, 354, 404
HhaI GCGC 2 cut(s) 14, 447
Hin6I GCGC 2 cut(s) 12, 445
HinP1I GCGC 2 cut(s) 12, 445
HincII GTYRAC 1 cut(s) 598
HindII GTYRAC 1 cut(s) 598
HpaII CCGG 4 cut(s) 333, 341, 354, 404
HphI GGTGA 2 cut(s) 392, 446
Hpy166II GTNNAC 2 cut(s) 169, 598
Hpy188III TCNNGA 1 cut(s) 374
Hpy8I GTNNAC 2 cut(s) 169, 598
HpyAV CCTTC 2 cut(s) 149, 553
HpyCH4III ACNGT 3 cut(s) 40, 121, 166
HpyCH4V TGCA 3 cut(s) 140, 428, 480
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 87, 279, 553
HpyF3I CTNAG 1 cut(s) 172
HspAI GCGC 2 cut(s) 12, 445
KpnI GGTACC 1 cut(s) 416
KroI GCCGGC 1 cut(s) 353
KroNI GCCGGC 1 cut(s) 355
Ksp22I TGATCA 1 cut(s) 397
Kzo9I GATC 4 cut(s) 306, 367, 397, 525
LguI GCTCTTC 1 cut(s) 432
LmnI GCTCC 1 cut(s) 334
Lsp1109I GCAGC 3 cut(s) 429, 492, 508
LweI GCATC 2 cut(s) 508, 578
MaeI CTAG 2 cut(s) 593, 620
MaeIII GTNAC 2 cut(s) 34, 418
MalI GATC 4 cut(s) 308, 369, 399, 527
MboI GATC 4 cut(s) 306, 367, 397, 525
MboII GAAGA 4 cut(s) 73, 362, 449, 580
MflI RGATCY 2 cut(s) 306, 367
MluCI AATT 1 cut(s) 362
MnlI CCTC 7 cut(s) 193, 210, 227, 370, 591, 606, 609
MroNI GCCGGC 1 cut(s) 353
MslI CAYNNNNRTG 1 cut(s) 164
MspI CCGG 4 cut(s) 333, 341, 354, 404
MspR9I CCNGG 3 cut(s) 333, 404, 405
Mva1269I GAATGC 1 cut(s) 482
MwoI GCNNNNNNNGC 4 cut(s) 11, 87, 279, 553
NaeI GCCGGC 1 cut(s) 355
NciI CCSGG 3 cut(s) 333, 404, 405
NdeII GATC 4 cut(s) 306, 367, 397, 525
NgoMIV GCCGGC 1 cut(s) 353
NlaIV GGNNCC 3 cut(s) 160, 308, 414
NmuCI GTSAC 2 cut(s) 34, 418
NsbI TGCGCA 1 cut(s) 446
OliI CACNNNNGTG 1 cut(s) 164
PciSI GCTCTTC 1 cut(s) 432
PctI GAATGC 1 cut(s) 482
PdiI GCCGGC 1 cut(s) 355
PfoI TCCNGGA 1 cut(s) 331
PkrI GCNGC 5 cut(s) 444, 482, 523, 546, 549
PspFI CCCAGC 1 cut(s) 191
PspN4I GGNNCC 3 cut(s) 160, 308, 414
PsuI RGATCY 2 cut(s) 306, 367
RsaI GTAC 2 cut(s) 348, 414
RsaNI GTAC 2 cut(s) 347, 413
RseI CAYNNNNRTG 1 cut(s) 164
SapI GCTCTTC 1 cut(s) 432
SatI GCNGC 5 cut(s) 443, 481, 522, 545, 548
Sau3AI GATC 4 cut(s) 306, 367, 397, 525
ScaI AGTACT 1 cut(s) 348
ScrFI CCNGG 3 cut(s) 333, 404, 405
SetI ASST 8 cut(s) 23, 68, 230, 284, 418, 444, 485, 583
SfaNI GCATC 2 cut(s) 508, 578
SfcI CTRYAG 1 cut(s) 229
SmaI CCCGGG 1 cut(s) 405
SmiMI CAYNNNNRTG 1 cut(s) 164
SmlI CTYRAG 1 cut(s) 629
SmoI CTYRAG 1 cut(s) 629
Sse9I AATT 1 cut(s) 362
SsiI CCGC 3 cut(s) 545, 548, 609
SspMI CTAG 2 cut(s) 593, 620
StyD4I CCNGG 3 cut(s) 331, 402, 403
TaaI ACNGT 3 cut(s) 40, 121, 166
TaqI TCGA 2 cut(s) 373, 516
TasI AATT 1 cut(s) 362
TatI WGTACW 1 cut(s) 346
TauI GCSGC 2 cut(s) 547, 550
TscAI CASTG 5 cut(s) 109, 142, 169, 193, 430
TseFI GTSAC 2 cut(s) 34, 418
TseI GCWGC 3 cut(s) 442, 480, 521
Tsp45I GTSAC 2 cut(s) 34, 418
TspDTI ATGAA 1 cut(s) 13
TspMI CCCGGG 1 cut(s) 403
TspRI CASTG 5 cut(s) 109, 142, 169, 193, 430
XmaI CCCGGG 1 cut(s) 403
XspI CTAG 2 cut(s) 593, 620
ZrmI AGTACT 1 cut(s) 348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.