MD14G1205500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0012489
Physical Location & Seq
Forward (+)
29296839 .. 29297813
975 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1205500.v1.1.491

Sequence Viewer

Length: 975 bp
ATGGAAAAGGCAGGCAGGATTATCAGAAAATCAGTCCACACATTCCTTCTAAACTACCAATACTTCACCTCAATTGCAGCAGTCATTGCCTTACCCTTCTCAGCCTCAGTTCTCATCTCACAAGCACTTCTTCCGTCTAGCTTCGCCCTCTTGCTCGCCGTCTACAACCGCCTGCACAGCCTTTTTGACGCGGCCGGCTTCCCTCCCTCCTTGGAATTCTTCACCATTCTCAACAACAAGCTCTCCCAAACCATCACCTCCTCAATCTTCACATTCCCATTCTCCCTCACCTTCCTCCTCATAACCAAAGCTTTTGTAATCCAATCTCTCAGCCACCACCAAAAACCGAATACCTCGTCACACCCATCGTTTCTTTCCACAATTTCTCTCTACACCCCACTCCTCCACACTCATGTCTACAACTCGTTTTTGATCCTCTCTGCCAATGCAACAGTGTTTTCTCTCTTGTTCATCGCCTTCAATGTCCTCGAGGGACTCGGTTTCTCTTCTCCGAACACCCTCCTCTTCTTATCGGTATCTGGGGCAGTTCTGTACTCCATTGTTCTTGCCAATGCGCTCATAATATGCAATCTTGCATTGGTTCTATCAGGTGCACAAAAGAGTGGCGGTTACTTGGCAATTCTCAAGGCCTGTCTTTTGATCAGAGGAAGAACTTCAACGGCTCTCTGTCTTGCTCTGCCTGTCAGCTTAACCCTAGCTGCAGTTGAAGCATTGTTCCAATACCGCATCGTACGAGCTTATCATTTCGCAGGAAAGCTCGGATCTTCCATGGCTATGGAGGGTGTTTTCCTTGCTTATTTGTACTCAATTCTTGTTGTTCTTGATATTACTGTGAGCTTTATGTTCTTACAAAGCTGCAAGCCGAGTTCTCGACGGATCGAACACAGAGACGACGAAGAAAGTCGTGGAGATTTGAAGGCTGTCAAAAGTGCTTCCATGAAAGTGCTTCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

325

Amino Acids

35.39

Weight (kDa)

9.54

Isoelectric Point (pI)

36.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012489)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G61340 AT5G61340
fragaria_vesca FvH4_5g14500
malus_domestica MD06G1197900.v1.1 MD14G1205500.v1.1
prunus_persica Prupe.5G198800_v2.0.a1 Prupe.5G198800_v2.0.a1
pyrus_communis pycom06g17490 pycom14g17000
rosa_chinensis RchiOBHm_Chr7g0182161
rosa_laevigata RLG00000005145
rosa_multiflora Rmu_sc0000003.1_g000019
rosa_roxburghii Rroxscaffold_3G00271460
rosa_rugosa Rorug06G0445800
rosa_samantha Rh7AG048800 Rh7BG048700 Rh7CG050100 Rh7DG048000
rosa_wichuraiana Rw7G003980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 488
AccI GTMKAC 2 cut(s) 162, 417
AccII CGCG 1 cut(s) 191
AciI CCGC 4 cut(s) 169, 191, 627, 745
AclWI GGATC 3 cut(s) 427, 790, 905
AcoI YGGCCR 1 cut(s) 192
AcsI RAATTY 1 cut(s) 215
AfaI GTAC 3 cut(s) 554, 753, 824
AgsI TTSAA 4 cut(s) 481, 678, 728, 937
AluBI AGCT 9 cut(s) 141, 241, 311, 708, 719, 758, 778, 858, 876
AluI AGCT 9 cut(s) 141, 241, 311, 708, 719, 758, 778, 858, 876
Alw21I GWGCWC 1 cut(s) 616
Alw26I GTCTC 1 cut(s) 903
Alw44I GTGCAC 1 cut(s) 612
AlwI GGATC 3 cut(s) 427, 790, 905
Ama87I CYCGRG 1 cut(s) 488
AoxI GGCC 2 cut(s) 192, 648
ApaLI GTGCAC 1 cut(s) 612
ApeKI GCWGC 3 cut(s) 77, 719, 876
ApoI RAATTY 1 cut(s) 215
ArsI GACNNNNNNTTYG 2 cut(s) 341, 373
Asp700I GAANNNNTTC 1 cut(s) 673
AspLEI GCGC 1 cut(s) 577
AsuHPI GGTGA 4 cut(s) 58, 214, 247, 280
AvaI CYCGRG 1 cut(s) 488
BaeGI GKGCMC 1 cut(s) 616
Bbv12I GWGCWC 1 cut(s) 616
BbvI GCAGC 3 cut(s) 89, 706, 863
BccI CCATC 2 cut(s) 260, 373
BceAI ACGGC 2 cut(s) 143, 696
BclI TGATCA 1 cut(s) 660
BcoDI GTCTC 1 cut(s) 903
BfaI CTAG 2 cut(s) 138, 716
BfmI CTRYAG 1 cut(s) 720
BisI GCNGC 4 cut(s) 78, 192, 720, 877
BlsI GCNGC 4 cut(s) 79, 193, 721, 878
BmeT110I CYCGRG 1 cut(s) 488
BmsI GCATC 1 cut(s) 756
BpuEI CTTGAG 1 cut(s) 629
BsaJI CCNNGG 2 cut(s) 210, 789
BsaXI ACNNNNNCTCC 2 cut(s) 227, 257
Bse118I RCCGGY 1 cut(s) 194
Bse3DI GCAATG 1 cut(s) 84
BseDI CCNNGG 2 cut(s) 210, 789
BseMI GCAATG 1 cut(s) 84
BseMII CTCAG 3 cut(s) 114, 120, 343
BseRI GAGGAG 4 cut(s) 250, 287, 392, 512
BseSI GKGCMC 1 cut(s) 616
BseX3I CGGCCG 1 cut(s) 192
BseXI GCAGC 3 cut(s) 89, 706, 863
BsgI GTGCAG 1 cut(s) 158
Bsh1236I CGCG 1 cut(s) 191
Bsh1285I CGRYCG 1 cut(s) 195
BshFI GGCC 2 cut(s) 194, 650
BsiEI CGRYCG 1 cut(s) 195
BsiHKAI GWGCWC 1 cut(s) 616
BsiHKCI CYCGRG 1 cut(s) 488
BsiSI CCGG 1 cut(s) 195
BsiWI CGTACG 1 cut(s) 751
BslFI GGGAC 1 cut(s) 507
BsmAI GTCTC 1 cut(s) 903
BsmBI CGTCTC 1 cut(s) 903
BsmFI GGGAC 1 cut(s) 507
BsnI GGCC 2 cut(s) 194, 650
BsoBI CYCGRG 1 cut(s) 488
Bsp1286I GDGCHC 1 cut(s) 616
Bsp143I GATC 4 cut(s) 432, 660, 782, 897
Bsp19I CCATGG 1 cut(s) 789
BspACI CCGC 4 cut(s) 169, 191, 627, 745
BspANI GGCC 2 cut(s) 194, 650
BspCNI CTCAG 3 cut(s) 113, 119, 342
BspFNI CGCG 1 cut(s) 191
BspMAI CTGCAG 1 cut(s) 724
BspPI GGATC 3 cut(s) 427, 790, 905
BsrDI GCAATG 1 cut(s) 84
BsrFI RCCGGY 1 cut(s) 194
BssAI RCCGGY 1 cut(s) 194
BssECI CCNNGG 2 cut(s) 210, 789
BssMI GATC 4 cut(s) 432, 660, 782, 897
BssT1I CCWWGG 2 cut(s) 210, 789
Bst4CI ACNGT 2 cut(s) 454, 853
Bst6I CTCTTC 2 cut(s) 511, 530
BstAPI GCANNNNNTGC 1 cut(s) 86
BstC8I GCNNGC 5 cut(s) 13, 156, 173, 196, 881
BstDEI CTNAG 3 cut(s) 100, 106, 329
BstDSI CCRYGG 1 cut(s) 789
BstFNI CGCG 1 cut(s) 191
BstHHI GCGC 1 cut(s) 577
BstKTI GATC 4 cut(s) 435, 663, 785, 900
BstMAI GTCTC 1 cut(s) 903
BstMBI GATC 4 cut(s) 432, 660, 782, 897
BstMCI CGRYCG 1 cut(s) 195
BstMWI GCNNNNNNNGC 3 cut(s) 86, 177, 728
BstSFI CTRYAG 1 cut(s) 720
BstSLI GKGCMC 1 cut(s) 616
BstUI CGCG 1 cut(s) 191
BstV1I GCAGC 3 cut(s) 89, 706, 863
BstX2I RGATCY 1 cut(s) 782
BstXI CCANNNNNNTGG 1 cut(s) 796
BstYI RGATCY 1 cut(s) 782
BstZI CGGCCG 1 cut(s) 192
BsuRI GGCC 2 cut(s) 194, 650
BtgI CCRYGG 1 cut(s) 789
BtgZI GCGATG 1 cut(s) 457
BtsIMutI CAGTG 1 cut(s) 459
Cac8I GCNNGC 5 cut(s) 13, 156, 173, 196, 881
CfoI GCGC 1 cut(s) 577
Cfr10I RCCGGY 1 cut(s) 194
CseI GACGC 1 cut(s) 197
Csp6I GTAC 3 cut(s) 553, 752, 823
CviAII CATG 4 cut(s) 413, 790, 958, 972
CviQI GTAC 3 cut(s) 553, 752, 823
DdeI CTNAG 3 cut(s) 100, 106, 329
DpnI GATC 4 cut(s) 434, 662, 784, 899
DpnII GATC 4 cut(s) 432, 660, 782, 897
EaeI YGGCCR 1 cut(s) 192
EagI CGGCCG 1 cut(s) 192
Eam1104I CTCTTC 2 cut(s) 511, 530
EarI CTCTTC 2 cut(s) 511, 530
EclXI CGGCCG 1 cut(s) 192
Eco130I CCWWGG 2 cut(s) 210, 789
Eco147I AGGCCT 1 cut(s) 650
Eco52I CGGCCG 1 cut(s) 192
Eco88I CYCGRG 1 cut(s) 488
EcoRI GAATTC 1 cut(s) 215
EcoT14I CCWWGG 2 cut(s) 210, 789
ErhI CCWWGG 2 cut(s) 210, 789
Esp3I CGTCTC 1 cut(s) 903
FaeI CATG 4 cut(s) 416, 793, 961, 975
FaiI YATR 9 cut(s) 302, 414, 581, 586, 791, 797, 863, 959, 973
FalI AAGNNNNNCTT 2 cut(s) 114, 146
FaqI GGGAC 1 cut(s) 507
FatI CATG 4 cut(s) 412, 789, 957, 971
FbaI TGATCA 1 cut(s) 660
FblI GTMKAC 2 cut(s) 162, 417
Fnu4HI GCNGC 4 cut(s) 78, 192, 720, 877
Fsp4HI GCNGC 4 cut(s) 78, 192, 720, 877
FspBI CTAG 2 cut(s) 138, 716
GlaI GCGC 1 cut(s) 576
GluI GCNGC 4 cut(s) 78, 192, 720, 877
HaeIII GGCC 2 cut(s) 194, 650
HapII CCGG 1 cut(s) 195
HgaI GACGC 1 cut(s) 197
HhaI GCGC 1 cut(s) 577
Hin1II CATG 4 cut(s) 416, 793, 961, 975
Hin6I GCGC 1 cut(s) 575
HinP1I GCGC 1 cut(s) 575
HindIII AAGCTT 1 cut(s) 309
HinfI GANTC 1 cut(s) 495
HpaII CCGG 1 cut(s) 195
HphI GGTGA 4 cut(s) 58, 214, 247, 280
Hpy166II GTNNAC 4 cut(s) 37, 163, 418, 614
Hpy188I TCNGA 4 cut(s) 26, 513, 665, 782
Hpy188III TCNNGA 2 cut(s) 842, 891
Hpy8I GTNNAC 4 cut(s) 37, 163, 418, 614
Hpy99I CGWCG 2 cut(s) 897, 917
HpyAV CCTTC 5 cut(s) 56, 106, 301, 487, 931
HpyCH4III ACNGT 2 cut(s) 454, 853
HpyCH4V TGCA 8 cut(s) 77, 175, 449, 588, 596, 614, 722, 879
HpyF10VI GCNNNNNNNGC 3 cut(s) 86, 177, 728
HpyF3I CTNAG 3 cut(s) 100, 106, 329
Hsp92II CATG 4 cut(s) 416, 793, 961, 975
HspAI GCGC 1 cut(s) 575
KroI GCCGGC 1 cut(s) 194
KroNI GCCGGC 1 cut(s) 196
Ksp22I TGATCA 1 cut(s) 660
Kzo9I GATC 4 cut(s) 432, 660, 782, 897
LpnPI CCDG 7 cut(s) 185, 208, 525, 594, 664, 714, 756
Lsp1109I GCAGC 3 cut(s) 89, 706, 863
LweI GCATC 1 cut(s) 756
MaeI CTAG 2 cut(s) 138, 716
MaeIII GTNAC 2 cut(s) 357, 629
MalI GATC 4 cut(s) 434, 662, 784, 899
MboI GATC 4 cut(s) 432, 660, 782, 897
MboII GAAGA 8 cut(s) 122, 211, 259, 498, 517, 681, 777, 929
MfeI CAATTG 1 cut(s) 72
MflI RGATCY 1 cut(s) 782
MhlI GDGCHC 1 cut(s) 616
MluCI AATT 5 cut(s) 72, 215, 381, 639, 828
MlyI GAGTC 1 cut(s) 489
MroNI GCCGGC 1 cut(s) 194
MroXI GAANNNNTTC 1 cut(s) 673
MseI TTAA 1 cut(s) 710
MslI CAYNNNNRTG 3 cut(s) 411, 794, 962
MspI CCGG 1 cut(s) 195
MunI CAATTG 1 cut(s) 72
MvnI CGCG 1 cut(s) 191
MwoI GCNNNNNNNGC 3 cut(s) 86, 177, 728
NaeI GCCGGC 1 cut(s) 196
NcoI CCATGG 1 cut(s) 789
NdeII GATC 4 cut(s) 432, 660, 782, 897
NgoMIV GCCGGC 1 cut(s) 194
NlaIII CATG 4 cut(s) 416, 793, 961, 975
NmeAIII GCCGAG 1 cut(s) 909
NmuCI GTSAC 1 cut(s) 357
PaeR7I CTCGAG 1 cut(s) 488
PceI AGGCCT 1 cut(s) 650
PdiI GCCGGC 1 cut(s) 196
PdmI GAANNNNTTC 1 cut(s) 673
Pfl23II CGTACG 1 cut(s) 751
PkrI GCNGC 4 cut(s) 79, 193, 721, 878
PleI GAGTC 1 cut(s) 489
PpsI GAGTC 1 cut(s) 489
PspLI CGTACG 1 cut(s) 751
PspXI VCTCGAGB 1 cut(s) 488
PstI CTGCAG 1 cut(s) 724
PsuI RGATCY 1 cut(s) 782
RsaI GTAC 3 cut(s) 554, 753, 824
RsaNI GTAC 3 cut(s) 553, 752, 823
RseI CAYNNNNRTG 3 cut(s) 411, 794, 962
SaqAI TTAA 1 cut(s) 710
SatI GCNGC 4 cut(s) 78, 192, 720, 877
Sau3AI GATC 4 cut(s) 432, 660, 782, 897
SchI GAGTC 1 cut(s) 489
SduI GDGCHC 1 cut(s) 616
SfaNI GCATC 1 cut(s) 756
SfcI CTRYAG 1 cut(s) 720
Sfr274I CTCGAG 1 cut(s) 488
SlaI CTCGAG 1 cut(s) 488
SmiMI CAYNNNNRTG 3 cut(s) 411, 794, 962
SmlI CTYRAG 2 cut(s) 488, 644
SmoI CTYRAG 2 cut(s) 488, 644
Sse9I AATT 5 cut(s) 72, 215, 381, 639, 828
SseBI AGGCCT 1 cut(s) 650
SsiI CCGC 4 cut(s) 169, 191, 627, 745
SspMI CTAG 2 cut(s) 138, 716
StuI AGGCCT 1 cut(s) 650
StyI CCWWGG 2 cut(s) 210, 789
TaaI ACNGT 2 cut(s) 454, 853
TaqI TCGA 3 cut(s) 489, 892, 900
TasI AATT 5 cut(s) 72, 215, 381, 639, 828
TatI WGTACW 2 cut(s) 552, 822
TauI GCSGC 1 cut(s) 194
Tru1I TTAA 1 cut(s) 710
Tru9I TTAA 1 cut(s) 710
TscAI CASTG 1 cut(s) 459
TseFI GTSAC 1 cut(s) 357
TseI GCWGC 3 cut(s) 77, 719, 876
Tsp45I GTSAC 1 cut(s) 357
TspDTI ATGAA 2 cut(s) 460, 974
TspGWI ACGGA 2 cut(s) 123, 910
TspRI CASTG 1 cut(s) 459
VneI GTGCAC 1 cut(s) 612
XapI RAATTY 1 cut(s) 215
XhoI CTCGAG 1 cut(s) 488
XmiI GTMKAC 2 cut(s) 162, 417
XmnI GAANNNNTTC 1 cut(s) 673
XspI CTAG 2 cut(s) 138, 716
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.