MD14G1215000.v1.1

COBRA-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0019274
Physical Location & Seq
Reverse (-)
29866275 .. 29866848
574 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1215000.v1.1.491

Sequence Viewer

Length: 492 bp
ATGTGCCCAATTCGAGTCCATTGGCATGTGAAGGCTAACTACCGAGAGTATTGGCGCGTGAGGATTGCCGTCACAAACTTCAAGTACCGGATGAATTATACACAATGGACTCTTGTTGCTCAGCATCCAAATCTCAATAAACTTGCTAATGTTTCAAGCTTTCTCTATAAGCCACTCATTCACTACGACACAAACGACACTGGCATGTTTTACGGCATAAAAAATCTTAACAACCTACTAATGGAAGCTGGAACCGATGGACATGTTGAATCAGAGCTTATTCTCCAAAAGAACACGAAGGAATTCACATTGAAGCAAGGTTGGGCATTTCCATTGATGATTTACTTCAATGGTGATGAATGCAAGATGCCCCTTCCTGATATATATCCATGCCTACCCAACTCTACTAATTCAAATCCAATTCCATCGTCTACACTAGCTACCCTTTTGCTTTCAGCACTACTACTAGTCTTCTTCAGCTCCCGTCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
COBRA

Protein Analysis

164

Amino Acids

18.93

Weight (kDa)

8.98

Isoelectric Point (pI)

28.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COB_C PF25079 2 - 130 3.4e-37 COBRA family C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019274)

Species Orthologous Gene IDs
malus_domestica MD14G1215000.v1.1
pyrus_communis pycom12433g00020
rosa_chinensis RchiOBHm_Chr5g0059981 RchiOBHm_Chr7g0183441
rosa_multiflora Rmu_sc0000814.1_g000035 Rmu_sc0022860.1_g000001
rosa_samantha Rh5BG403600 Rh5CG427600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 431
AccII CGCG 1 cut(s) 57
AcsI RAATTY 1 cut(s) 302
AcuI CTGAAG 1 cut(s) 460
AfaI GTAC 1 cut(s) 86
AfiI CCNNNNNNNGG 1 cut(s) 241
AflIII ACRYGT 1 cut(s) 262
AgsI TTSAA 6 cut(s) 82, 156, 269, 313, 349, 414
AhlI ACTAGT 1 cut(s) 466
AluBI AGCT 5 cut(s) 159, 248, 277, 440, 480
AluI AGCT 5 cut(s) 159, 248, 277, 440, 480
ApoI RAATTY 1 cut(s) 302
Asp700I GAANNNNTTC 1 cut(s) 302
AspLEI GCGC 1 cut(s) 57
AsuHPI GGTGA 1 cut(s) 365
BaeGI GKGCMC 1 cut(s) 8
BarI GAAGNNNNNNTAC 2 cut(s) 23, 55
BbsI GAAGAC 1 cut(s) 463
BccI CCATC 2 cut(s) 251, 433
BceAI ACGGC 2 cut(s) 53, 229
BcuI ACTAGT 1 cut(s) 466
BfaI CTAG 2 cut(s) 437, 467
BlpI GCTNAGC 1 cut(s) 120
BmiI GGNNCC 1 cut(s) 253
BmsI GCATC 2 cut(s) 133, 357
BpiI GAAGAC 1 cut(s) 463
Bpu1102I GCTNAGC 1 cut(s) 120
BsaBI GATNNNNATC 1 cut(s) 384
BsaWI WCCGGW 1 cut(s) 87
Bsc4I CCNNNNNNNGG 1 cut(s) 241
Bse1I ACTGG 1 cut(s) 205
Bse8I GATNNNNATC 1 cut(s) 384
BseGI GGATG 2 cut(s) 96, 124
BseJI GATNNNNATC 1 cut(s) 384
BseLI CCNNNNNNNGG 1 cut(s) 241
BseMII CTCAG 1 cut(s) 134
BseNI ACTGG 1 cut(s) 205
BseSI GKGCMC 1 cut(s) 8
Bsh1236I CGCG 1 cut(s) 57
BsiSI CCGG 1 cut(s) 88
BslI CCNNNNNNNGG 1 cut(s) 241
BsmI GAATGC 1 cut(s) 365
Bsp1286I GDGCHC 1 cut(s) 8
Bsp1720I GCTNAGC 1 cut(s) 120
BspCNI CTCAG 1 cut(s) 133
BspFNI CGCG 1 cut(s) 57
BspLI GGNNCC 1 cut(s) 253
BsrI ACTGG 1 cut(s) 205
BstDEI CTNAG 1 cut(s) 120
BstF5I GGATG 2 cut(s) 96, 124
BstFNI CGCG 1 cut(s) 57
BstHHI GCGC 1 cut(s) 57
BstNSI RCATGY 3 cut(s) 29, 208, 266
BstSLI GKGCMC 1 cut(s) 8
BstUI CGCG 1 cut(s) 57
BstV2I GAAGAC 1 cut(s) 463
BtsCI GGATG 2 cut(s) 96, 124
BtsIMutI CAGTG 1 cut(s) 198
CfoI GCGC 1 cut(s) 57
Csp6I GTAC 1 cut(s) 85
CviAII CATG 4 cut(s) 26, 205, 263, 390
CviJI RGCY 7 cut(s) 35, 159, 172, 248, 277, 440, 480
CviKI_1 RGCY 7 cut(s) 35, 159, 172, 248, 277, 440, 480
CviQI GTAC 1 cut(s) 85
DdeI CTNAG 1 cut(s) 120
Eco57I CTGAAG 1 cut(s) 460
EcoRI GAATTC 1 cut(s) 302
FaeI CATG 4 cut(s) 29, 208, 266, 393
FaiI YATR 9 cut(s) 27, 99, 168, 206, 218, 264, 383, 385, 391
FatI CATG 4 cut(s) 25, 204, 262, 389
FblI GTMKAC 1 cut(s) 431
FokI GGATG 2 cut(s) 103, 111
FspBI CTAG 2 cut(s) 437, 467
GlaI GCGC 1 cut(s) 56
HapII CCGG 1 cut(s) 88
HhaI GCGC 1 cut(s) 57
Hin1II CATG 4 cut(s) 29, 208, 266, 393
Hin6I GCGC 1 cut(s) 55
HinP1I GCGC 1 cut(s) 55
HindIII AAGCTT 1 cut(s) 157
HinfI GANTC 3 cut(s) 15, 109, 269
HpaII CCGG 1 cut(s) 88
HphI GGTGA 1 cut(s) 365
Hpy166II GTNNAC 1 cut(s) 432
Hpy188I TCNGA 1 cut(s) 274
Hpy188III TCNNGA 1 cut(s) 377
Hpy8I GTNNAC 1 cut(s) 432
HpyAV CCTTC 3 cut(s) 25, 292, 383
HpyCH4V TGCA 1 cut(s) 363
HpyF3I CTNAG 1 cut(s) 120
Hsp92II CATG 4 cut(s) 29, 208, 266, 393
HspAI GCGC 1 cut(s) 55
LmnI GCTCC 1 cut(s) 485
LpnPI CCDG 4 cut(s) 101, 186, 234, 390
LweI GCATC 2 cut(s) 133, 357
MaeI CTAG 2 cut(s) 437, 467
MaeIII GTNAC 2 cut(s) 70, 485
MboII GAAGA 2 cut(s) 463, 466
MhlI GDGCHC 1 cut(s) 8
MluCI AATT 5 cut(s) 9, 94, 302, 409, 420
MlyI GAGTC 2 cut(s) 24, 103
MnlI CCTC 1 cut(s) 54
MroXI GAANNNNTTC 1 cut(s) 302
MseI TTAA 1 cut(s) 228
MslI CAYNNNNRTG 2 cut(s) 24, 203
MspI CCGG 1 cut(s) 88
Mva1269I GAATGC 1 cut(s) 365
MvnI CGCG 1 cut(s) 57
NlaIII CATG 4 cut(s) 29, 208, 266, 393
NlaIV GGNNCC 1 cut(s) 253
NmuCI GTSAC 2 cut(s) 70, 485
NspI RCATGY 3 cut(s) 29, 208, 266
PciI ACATGT 1 cut(s) 262
PcsI WCGNNNNNNNCGW 1 cut(s) 192
PctI GAATGC 1 cut(s) 365
PdmI GAANNNNTTC 1 cut(s) 302
PfeI GAWTC 1 cut(s) 269
PleI GAGTC 2 cut(s) 23, 103
PpsI GAGTC 2 cut(s) 23, 103
PscI ACATGT 1 cut(s) 262
PspN4I GGNNCC 1 cut(s) 253
RsaI GTAC 1 cut(s) 86
RsaNI GTAC 1 cut(s) 85
RseI CAYNNNNRTG 2 cut(s) 24, 203
SaqAI TTAA 1 cut(s) 228
SchI GAGTC 2 cut(s) 24, 103
SduI GDGCHC 1 cut(s) 8
SetI ASST 7 cut(s) 161, 237, 250, 279, 322, 442, 482
SfaNI GCATC 2 cut(s) 133, 357
SmiMI CAYNNNNRTG 2 cut(s) 24, 203
SpeI ACTAGT 1 cut(s) 466
Sse9I AATT 5 cut(s) 9, 94, 302, 409, 420
SspMI CTAG 2 cut(s) 437, 467
TaqI TCGA 1 cut(s) 13
TasI AATT 5 cut(s) 9, 94, 302, 409, 420
TfiI GAWTC 1 cut(s) 269
Tru1I TTAA 1 cut(s) 228
Tru9I TTAA 1 cut(s) 228
TscAI CASTG 1 cut(s) 205
TseFI GTSAC 2 cut(s) 70, 485
Tsp45I GTSAC 2 cut(s) 70, 485
TspDTI ATGAA 2 cut(s) 107, 372
TspRI CASTG 1 cut(s) 205
XapI RAATTY 1 cut(s) 302
XceI RCATGY 3 cut(s) 29, 208, 266
XmiI GTMKAC 1 cut(s) 431
XmnI GAANNNNTTC 1 cut(s) 302
XspI CTAG 2 cut(s) 437, 467
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.