pycom12433g00020

COBRA-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00012433
OG0019274
Physical Location & Seq
Forward (+)
14680 .. 14976
297 bp
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UTR
Exon/CDS
Intron
pycom12433g00020.1

Sequence Viewer

Length: 297 bp
ATGTTATATGCAGATGACACTGGCATGTTTTATGGCTTGAAGTTCTTCAATGACCACTTAATGGAAGCCGGGAAATTTGGGCACGTTCAGTCGGAGATGCTGCTTAAAAAGGACAAGAGCACCTTCACTTTGAGGGAGGGGTGGGCATTTCCTCGCAAAGTTTACTTCAATGGCGATGAGTGCCAGTTGCCTCCACCTGATGTCTACCCGTTTCTTCCCAATTCTGCCCATGAAATTCTACTTTCCTTCTCAACGTTGATTTCGTCATTCACTTTCGTGTTCACAGCTCTTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
COBRA

Protein Analysis

99

Amino Acids

11.34

Weight (kDa)

5.44

Isoelectric Point (pI)

37.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COB_C PF25079 3 - 70 3.8e-15 COBRA family C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019274)

Species Orthologous Gene IDs
malus_domestica MD14G1215000.v1.1
pyrus_communis pycom12433g00020
rosa_chinensis RchiOBHm_Chr5g0059981 RchiOBHm_Chr7g0183441
rosa_multiflora Rmu_sc0000814.1_g000035 Rmu_sc0022860.1_g000001
rosa_samantha Rh5BG403600 Rh5CG427600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 61
AccI GTMKAC 1 cut(s) 204
AclI AACGTT 1 cut(s) 254
AcsI RAATTY 2 cut(s) 74, 234
AfiI CCNNNNNNNGG 1 cut(s) 61
AgsI TTSAA 3 cut(s) 40, 49, 169
AleI CACNNNNGTG 1 cut(s) 275
AluBI AGCT 1 cut(s) 287
AluI AGCT 1 cut(s) 287
Alw21I GWGCWC 1 cut(s) 122
ApeKI GCWGC 1 cut(s) 100
ApoI RAATTY 2 cut(s) 74, 234
Asp700I GAANNNNTTC 1 cut(s) 44
AsuC2I CCSGG 1 cut(s) 70
BaeGI GKGCMC 1 cut(s) 84
Bbv12I GWGCWC 1 cut(s) 122
BbvI GCAGC 1 cut(s) 87
BcnI CCSGG 1 cut(s) 70
BisI GCNGC 1 cut(s) 101
BlsI GCNGC 1 cut(s) 102
Bme1390I CCNGG 1 cut(s) 70
BmrFI CCNGG 1 cut(s) 70
BmsI GCATC 1 cut(s) 87
BpuMI CCSGG 1 cut(s) 70
Bsc4I CCNNNNNNNGG 1 cut(s) 61
Bse1I ACTGG 2 cut(s) 25, 184
BseLI CCNNNNNNNGG 1 cut(s) 61
BseNI ACTGG 2 cut(s) 25, 184
BseSI GKGCMC 1 cut(s) 84
BseXI GCAGC 1 cut(s) 87
BsiHKAI GWGCWC 1 cut(s) 122
BsiSI CCGG 1 cut(s) 69
BslI CCNNNNNNNGG 1 cut(s) 61
Bsp1286I GDGCHC 2 cut(s) 84, 122
BsrI ACTGG 2 cut(s) 25, 184
BstMWI GCNNNNNNNGC 1 cut(s) 180
BstNSI RCATGY 1 cut(s) 28
BstSCI CCNGG 1 cut(s) 68
BstSLI GKGCMC 1 cut(s) 84
BstV1I GCAGC 1 cut(s) 87
BtgZI GCGATG 1 cut(s) 189
BtsIMutI CAGTG 1 cut(s) 18
CviAII CATG 2 cut(s) 25, 230
CviJI RGCY 3 cut(s) 36, 68, 287
CviKI_1 RGCY 3 cut(s) 36, 68, 287
FaeI CATG 2 cut(s) 28, 233
FaiI YATR 5 cut(s) 7, 9, 26, 33, 231
FalI AAGNNNNNCTT 2 cut(s) 107, 139
FatI CATG 2 cut(s) 24, 229
FblI GTMKAC 1 cut(s) 204
Fnu4HI GCNGC 1 cut(s) 101
Fsp4HI GCNGC 1 cut(s) 101
GluI GCNGC 1 cut(s) 101
HapII CCGG 1 cut(s) 69
Hin1II CATG 2 cut(s) 28, 233
HpaII CCGG 1 cut(s) 69
Hpy166II GTNNAC 3 cut(s) 163, 205, 282
Hpy188I TCNGA 1 cut(s) 94
Hpy8I GTNNAC 3 cut(s) 163, 205, 282
HpyAV CCTTC 2 cut(s) 133, 256
HpyCH4IV ACGT 2 cut(s) 84, 254
HpyCH4V TGCA 1 cut(s) 11
HpyF10VI GCNNNNNNNGC 1 cut(s) 180
HpySE526I ACGT 2 cut(s) 84, 254
Hsp92II CATG 2 cut(s) 28, 233
LpnPI CCDG 4 cut(s) 6, 82, 197, 210
Lsp1109I GCAGC 1 cut(s) 87
LweI GCATC 1 cut(s) 87
MaeII ACGT 2 cut(s) 84, 254
MboII GAAGA 2 cut(s) 37, 206
MhlI GDGCHC 2 cut(s) 84, 122
MluCI AATT 3 cut(s) 74, 220, 234
MmeI TCCRAC 1 cut(s) 72
MnlI CCTC 4 cut(s) 126, 130, 162, 201
MroXI GAANNNNTTC 1 cut(s) 44
MseI TTAA 2 cut(s) 59, 105
MslI CAYNNNNRTG 2 cut(s) 23, 275
MspI CCGG 1 cut(s) 69
MspR9I CCNGG 1 cut(s) 70
MwoI GCNNNNNNNGC 1 cut(s) 180
NciI CCSGG 1 cut(s) 70
NlaIII CATG 2 cut(s) 28, 233
NspI RCATGY 1 cut(s) 28
OliI CACNNNNGTG 1 cut(s) 275
PcsI WCGNNNNNNNCGW 1 cut(s) 260
PdmI GAANNNNTTC 1 cut(s) 44
PflMI CCANNNNNTGG 1 cut(s) 61
PkrI GCNGC 1 cut(s) 102
Psp1406I AACGTT 1 cut(s) 254
RseI CAYNNNNRTG 2 cut(s) 23, 275
SaqAI TTAA 2 cut(s) 59, 105
SatI GCNGC 1 cut(s) 101
ScrFI CCNGG 1 cut(s) 70
SduI GDGCHC 2 cut(s) 84, 122
SetI ASST 5 cut(s) 87, 125, 199, 257, 289
SfaNI GCATC 1 cut(s) 87
SmiMI CAYNNNNRTG 2 cut(s) 23, 275
Sse9I AATT 3 cut(s) 74, 220, 234
StyD4I CCNGG 1 cut(s) 68
TaiI ACGT 2 cut(s) 87, 257
TasI AATT 3 cut(s) 74, 220, 234
Tru1I TTAA 2 cut(s) 59, 105
Tru9I TTAA 2 cut(s) 59, 105
TscAI CASTG 1 cut(s) 25
TseI GCWGC 1 cut(s) 100
TspDTI ATGAA 1 cut(s) 246
TspRI CASTG 1 cut(s) 25
Van91I CCANNNNNTGG 1 cut(s) 61
XapI RAATTY 2 cut(s) 74, 234
XceI RCATGY 1 cut(s) 28
XmiI GTMKAC 1 cut(s) 204
XmnI GAANNNNTTC 1 cut(s) 44
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.