MD14G1218900.v1.1

Acyl-activating enzyme 17

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0010059
Physical Location & Seq
Reverse (-)
30165628 .. 30171454
5827 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1218900.v1.1.491

Sequence Viewer

Length: 2187 bp
ATGATGGCGTATAAGAGCCTGGACGGCGTTACAATTTCCGACATCGAAGTTCTGGGGATCGAATCGGGTGCTGCGAAGAGACTCCATGGAAGTTTGACTGATATCATCGCTAATTACGGAGCCGCCACACCGGAGACGTGGCGCAATATCACTGCGCACGTCCTCAGCCCCGAGCTTCCCTTCTCGTTCCACCGGATGTTGTACTACGGCTGCTACAAGGACTTCGGACCCGACCCGCCCGCCTGGATGCCCGACCCGGAGACTGCAGGACTAACAAATGTTGGCCAGCTACTTGAGAGGAGGGGAAAAGAGCTCTTGGGTTCCAGATACAGGGATCCCATATCCAGCTTTTCGGATTTTCAGGAATTTTCGGTGTCAAACCCTGAGGTTTATTGGAAAACTGTACTACTAGATGAGATGAACGCATCCTTTGATACACCTCCCCAATGCATTTTACGTGAGAATTTGTCTGGAGATGGGCATTTGTTAGTTCCTGGAGGTCAATGGCTTCCTGGAGCGTTTGGGAATCCGGCCAAAAATTGCTTGATTGTAAATCGTAAGAGGAGCTTGAATGATACCATGGTGATATGGCGCGATGAAGGAAATGATGATCTGCCTTTGAATAAGATGACGCTTAAAGAATTGCGCACAGAGGTTTGGTTAGTTGCACATGCGCTAAAAGCACTGGGTTTGGAGAAAGGCTCTGCAATCGCAATTGATATGCCGATGCATGTCAATGCCATAGTTATTTACCTTGCTATTGTTCTGGCAGGATGTGTTGTTGTATCAATCGCTGATAGTTTTGCTCCTCCTGAAATATCAACAAGGCTTAAAATATCAGAGGCAAAAGCCATATTTACCCAGGATCTAATAAAACGCGGCGAGAAAAGTCTGCCCTTGTATAGTAAAATTGTGGCAGCAGAGTCTCCTATGGCAATCGTTATTCTTACCAAAGGTTCCAGTTCTAGCACGAAATTGCGTGATGGTGACATTTCCTGGCATGATTTTCTAGAAACAGTAAAAGATTTCAAAGACAATGAATTTGCTGCTGTAGAACAACCCATAGAAGCATTTACAAATATTCTCTTCTCATCTGGAACTACTGGGGATCCAAAGGCAATTCCATGGACCCTTGCAACTCCTTTCAAAGCTGCTGCAGATGCCTGGTGTCACATGGACATCCGTATAGGTGACGTTGTTGCTTGGCCCACAAATCTTGGGTGGATGATGGGCCCTTGGCTGGTATATGCTTCATTGTTAAATGGAGCTTCCATTGCCTTATATAATGGATCTCCACTTGGATCCGGCTTTGCCAAGTTTGTTCAGGATGCCAAAGTAACAATGCTTGGTGTAATCCCGAGCATTGTTCGGTCATGGAAAAGTACAAATTCTGTTTCCGGCTATGATTGGTCTGCCATTCGTTGCTTTGGATCCACCGGCGAAGCATCAAATGTAGAGGAATACCTATGGCTGATGGGGAGGGCTCACTACAAGCCTATCATTGAGTACTGTGGTGGTACAGAGATTGGTGGTGGTTTTGTTACTGGGTCGTTGTTGCAGGCTCAGTCGTTGGCTGCTTTTAGCACACCAGCTATGGGCTGCAGTTTGTATATTCTTGGCAATGATGGTGTTCCTATTCCACAAAATGAGCCAGGGGTTGGTGAATTGGCGCTTGATCCCGTCATGTTTGGAGCTTCTAGTACATTACTGAATGCTAATCATTATGACGTTTATTTCAAGGGAATGCCAATTTGGAATGGAAAGGTGTTAAGGAGGCATGGAGATGTGTTTGAACGGACATCTAGAGGTTACTATCATGCGCACGGTCGTGCAGATGATACAATGAATATTGGGGGCATCAAGGTTAGTTCGGTTGAGATTGAACGAATCTGTAATGCAGTTGATAACAATGTTCTCGAGACAGCTGCGATTGGAGTGCCGCTTGCTGGAGGCGGGCCTGAGCAGCTAGTAATTGCCGTCGTATTCAAGAACTCAGATAACCCAACTGCAGACTTGAATCAGTTGAGAATGTCCTTCAATTCAGCCGTGCAGAAGAAATTAAATCCGTTGTTCAAGGTTTCCAAAGTTGTGCCTCTCCCGTCTCTTCCAAGAACAGCAACAAATAAGGTCATGCGAAGGATTTTGCGGCTGCAGTTTGCTCAACTCGACCAAGGTCCTAAGCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ACAS_N AMP-binding AMP-binding_C

Protein Analysis

729

Amino Acids

79.41

Weight (kDa)

5.97

Isoelectric Point (pI)

38.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 208 - 556 5.3e-44 AMP-binding enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 3 cut(s) 156, 647, 1821
AccB7I CCANNNNNTGG 1 cut(s) 1658
AccII CGCG 2 cut(s) 594, 879
AciI CCGC 7 cut(s) 123, 236, 240, 879, 1940, 1953, 2146
AcoI YGGCCR 2 cut(s) 283, 531
AcsI RAATTY 4 cut(s) 365, 463, 1040, 1387
AfaI GTAC 6 cut(s) 203, 405, 1384, 1508, 1519, 1702
AfiI CCNNNNNNNGG 5 cut(s) 330, 1240, 1595, 1658, 1946
AjiI CACGTC 2 cut(s) 138, 160
AjnI CCWGG 8 cut(s) 18, 242, 493, 511, 861, 995, 1163, 1651
AjuI GAANNNNNNNTTGG 2 cut(s) 1735, 1767
AleI CACNNNNGTG 1 cut(s) 1827
Alw21I GWGCWC 1 cut(s) 315
Alw26I GTCTC 6 cut(s) 73, 128, 254, 930, 1913, 2106
Ama87I CYCGRG 3 cut(s) 170, 1357, 1916
AoxI GGCC 5 cut(s) 283, 531, 1205, 1231, 1955
ApaI GGGCCC 1 cut(s) 1235
ApoI RAATTY 4 cut(s) 365, 463, 1040, 1387
ArsI GACNNNNNNTTYG 2 cut(s) 1025, 1057
AspLEI GCGC 7 cut(s) 144, 157, 594, 648, 676, 1672, 1822
AspS9I GGNCC 7 cut(s) 227, 1128, 1206, 1231, 1232, 1955, 2174
AsuC2I CCSGG 1 cut(s) 257
AsuHPI GGTGA 4 cut(s) 595, 998, 1202, 1673
AvaI CYCGRG 3 cut(s) 170, 1357, 1916
AvaII GGWCC 3 cut(s) 227, 1128, 2174
AxyI CCTNAGG 1 cut(s) 384
BaeGI GKGCMC 1 cut(s) 1235
BaeI ACNNNNGTAYC 2 cut(s) 567, 600
BalI TGGCCA 1 cut(s) 285
BamHI GGATCC 4 cut(s) 334, 1108, 1301, 1430
BanII GRGCYC 3 cut(s) 315, 1235, 1486
Bbv12I GWGCWC 1 cut(s) 315
BbvCI CCTCAGC 1 cut(s) 164
BccI CCATC 5 cut(s) 470, 977, 1222, 1468, 1619
BceAI ACGGC 4 cut(s) 40, 223, 1961, 2030
BcgI CGANNNNNNTGC 6 cut(s) 50, 84, 1907, 1918, 1941, 1952
BciT130I CCWGG 8 cut(s) 20, 244, 495, 513, 863, 997, 1165, 1653
BcnI CCSGG 1 cut(s) 257
BcoDI GTCTC 6 cut(s) 73, 128, 254, 930, 1913, 2106
BfaI CTAG 6 cut(s) 410, 966, 1010, 1698, 1803, 1967
BfmI CTRYAG 6 cut(s) 264, 1050, 1155, 1600, 2007, 2150
BfoI RGCGCY 1 cut(s) 1673
BglI GCCNNNNNGGC 1 cut(s) 24
BmcAI AGTACT 1 cut(s) 1508
Bme1390I CCNGG 9 cut(s) 20, 244, 257, 495, 513, 863, 997, 1165, 1653
Bme18I GGWCC 3 cut(s) 227, 1128, 2174
BmeT110I CYCGRG 3 cut(s) 170, 1357, 1916
BmgBI CACGTC 2 cut(s) 138, 160
BmgT120I GGNCC 7 cut(s) 227, 1128, 1206, 1231, 1232, 1955, 2174
BmrFI CCNGG 9 cut(s) 20, 244, 257, 495, 513, 863, 997, 1165, 1653
BmrI ACTGGG 3 cut(s) 695, 1113, 1554
BmsI GCATC 7 cut(s) 237, 434, 717, 1150, 1318, 1454, 1866
BmuI ACTGGG 3 cut(s) 695, 1113, 1554
BoxI GACNNNNGTC 1 cut(s) 2172
BplI GAGNNNNNCTC 2 cut(s) 686, 718
BpmI CTGGAG 4 cut(s) 492, 516, 534, 1968
Bpu10I CCTNAGC 3 cut(s) 164, 1959, 2178
BpuEI CTTGAG 1 cut(s) 314
BpuMI CCSGG 1 cut(s) 257
BsaAI YACGTR 1 cut(s) 458
BsaJI CCNNGG 7 cut(s) 85, 579, 861, 1124, 1235, 1652, 2170
BsaWI WCCGGW 2 cut(s) 130, 192
Bsc4I CCNNNNNNNGG 5 cut(s) 330, 1240, 1595, 1658, 1946
Bse118I RCCGGY 1 cut(s) 1436
Bse1I ACTGG 4 cut(s) 690, 960, 1108, 1549
Bse21I CCTNAGG 1 cut(s) 384
Bse3DI GCAATG 2 cut(s) 1272, 1627
BseBI CCWGG 8 cut(s) 20, 244, 495, 513, 863, 997, 1165, 1653
BseDI CCNNGG 7 cut(s) 85, 579, 861, 1124, 1235, 1652, 2170
BseGI GGATG 7 cut(s) 201, 252, 425, 779, 1179, 1230, 1333
BseLI CCNNNNNNNGG 5 cut(s) 330, 1240, 1595, 1658, 1946
BseMI GCAATG 2 cut(s) 1272, 1627
BseMII CTCAG 5 cut(s) 178, 375, 1577, 1950, 2007
BseNI ACTGG 4 cut(s) 690, 960, 1108, 1549
BseRI GAGGAG 3 cut(s) 313, 577, 798
BseSI GKGCMC 1 cut(s) 1235
BsgI GTGCAG 2 cut(s) 1851, 2069
Bsh1236I CGCG 2 cut(s) 594, 879
Bsh1285I CGRYCG 1 cut(s) 1828
BshFI GGCC 5 cut(s) 285, 533, 1207, 1233, 1957
BsiEI CGRYCG 1 cut(s) 1828
BsiHKAI GWGCWC 1 cut(s) 315
BsiHKCI CYCGRG 3 cut(s) 170, 1357, 1916
BsiSI CCGG 7 cut(s) 131, 193, 257, 530, 1305, 1398, 1437
BslI CCNNNNNNNGG 5 cut(s) 330, 1240, 1595, 1658, 1946
BsmAI GTCTC 6 cut(s) 73, 128, 254, 930, 1913, 2106
BsmBI CGTCTC 2 cut(s) 128, 2106
BsmI GAATGC 2 cut(s) 1717, 1749
BsnI GGCC 5 cut(s) 285, 533, 1207, 1233, 1957
BsoBI CYCGRG 3 cut(s) 170, 1357, 1916
Bsp120I GGGCCC 1 cut(s) 1231
Bsp1286I GDGCHC 3 cut(s) 315, 1235, 1486
Bsp143I GATC 9 cut(s) 57, 334, 610, 865, 1108, 1289, 1301, 1430, 1675
Bsp19I CCATGG 3 cut(s) 85, 579, 1124
BspACI CCGC 7 cut(s) 123, 236, 240, 879, 1940, 1953, 2146
BspANI GGCC 5 cut(s) 285, 533, 1207, 1233, 1957
BspCNI CTCAG 5 cut(s) 177, 376, 1576, 1951, 2006
BspFNI CGCG 2 cut(s) 594, 879
BspMAI CTGCAG 5 cut(s) 268, 1159, 1604, 2011, 2154
BsrDI GCAATG 2 cut(s) 1272, 1627
BsrFI RCCGGY 1 cut(s) 1436
BsrI ACTGG 4 cut(s) 690, 960, 1108, 1549
BssAI RCCGGY 1 cut(s) 1436
BssECI CCNNGG 7 cut(s) 85, 579, 861, 1124, 1235, 1652, 2170
BssMI GATC 9 cut(s) 57, 334, 610, 865, 1108, 1289, 1301, 1430, 1675
BssT1I CCWWGG 5 cut(s) 85, 579, 1124, 1235, 2170
Bst2UI CCWGG 8 cut(s) 20, 244, 495, 513, 863, 997, 1165, 1653
Bst4CI ACNGT 4 cut(s) 403, 1018, 1511, 1826
Bst6I CTCTTC 3 cut(s) 71, 1091, 2109
BstBAI YACGTR 1 cut(s) 458
BstC8I GCNNGC 5 cut(s) 240, 287, 1560, 1944, 1955
BstDEI CTNAG 6 cut(s) 164, 384, 1563, 1959, 1993, 2178
BstDSI CCRYGG 3 cut(s) 85, 579, 1124
BstF5I GGATG 7 cut(s) 201, 252, 425, 779, 1179, 1230, 1333
BstFNI CGCG 2 cut(s) 594, 879
BstH2I RGCGCY 1 cut(s) 1673
BstHHI GCGC 7 cut(s) 144, 157, 594, 648, 676, 1672, 1822
BstKTI GATC 9 cut(s) 60, 337, 613, 868, 1111, 1292, 1304, 1433, 1678
BstMAI GTCTC 6 cut(s) 73, 128, 254, 930, 1913, 2106
BstMBI GATC 9 cut(s) 57, 334, 610, 865, 1108, 1289, 1301, 1430, 1675
BstMCI CGRYCG 1 cut(s) 1828
BstMWI GCNNNNNNNGC 5 cut(s) 24, 680, 1160, 1274, 1963
BstNI CCWGG 8 cut(s) 20, 244, 495, 513, 863, 997, 1165, 1653
BstNSI RCATGY 2 cut(s) 674, 734
BstPAI GACNNNNGTC 1 cut(s) 2172
BstSCI CCNGG 9 cut(s) 18, 242, 255, 493, 511, 861, 995, 1163, 1651
BstSFI CTRYAG 6 cut(s) 264, 1050, 1155, 1600, 2007, 2150
BstSLI GKGCMC 1 cut(s) 1235
BstUI CGCG 2 cut(s) 594, 879
BstX2I RGATCY 6 cut(s) 334, 865, 1108, 1289, 1301, 1430
BstYI RGATCY 6 cut(s) 334, 865, 1108, 1289, 1301, 1430
Bsu36I CCTNAGG 1 cut(s) 384
BsuRI GGCC 5 cut(s) 285, 533, 1207, 1233, 1957
BtgI CCRYGG 3 cut(s) 85, 579, 1124
BtgZI GCGATG 2 cut(s) 91, 609
BtrI CACGTC 2 cut(s) 138, 160
BtsCI GGATG 7 cut(s) 201, 252, 425, 779, 1179, 1230, 1333
BtsI GCAGTG 1 cut(s) 150
BtsIMutI CAGTG 2 cut(s) 150, 683
Cac8I GCNNGC 5 cut(s) 240, 287, 1560, 1944, 1955
CfoI GCGC 7 cut(s) 144, 157, 594, 648, 676, 1672, 1822
Cfr10I RCCGGY 1 cut(s) 1436
Cfr13I GGNCC 7 cut(s) 227, 1128, 1206, 1231, 1232, 1955, 2174
CseI GACGC 1 cut(s) 640
Csp6I GTAC 6 cut(s) 202, 404, 1383, 1507, 1518, 1701
CspCI CAANNNNNGTGG 2 cut(s) 1198, 1233
CviQI GTAC 6 cut(s) 202, 404, 1383, 1507, 1518, 1701
DdeI CTNAG 6 cut(s) 164, 384, 1563, 1959, 1993, 2178
DpnI GATC 9 cut(s) 59, 336, 612, 867, 1110, 1291, 1303, 1432, 1677
DpnII GATC 9 cut(s) 57, 334, 610, 865, 1108, 1289, 1301, 1430, 1675
EaeI YGGCCR 2 cut(s) 283, 531
Eam1104I CTCTTC 3 cut(s) 71, 1091, 2109
EarI CTCTTC 3 cut(s) 71, 1091, 2109
Ecl136II GAGCTC 1 cut(s) 313
Eco130I CCWWGG 5 cut(s) 85, 579, 1124, 1235, 2170
Eco24I GRGCYC 3 cut(s) 315, 1235, 1486
Eco32I GATATC 1 cut(s) 103
Eco47I GGWCC 3 cut(s) 227, 1128, 2174
Eco53kI GAGCTC 1 cut(s) 313
Eco81I CCTNAGG 1 cut(s) 384
Eco88I CYCGRG 3 cut(s) 170, 1357, 1916
EcoICRI GAGCTC 1 cut(s) 313
EcoO109I RGGNCCY 2 cut(s) 1232, 2174
EcoRII CCWGG 8 cut(s) 18, 242, 493, 511, 861, 995, 1163, 1651
EcoRV GATATC 1 cut(s) 103
EcoT14I CCWWGG 5 cut(s) 85, 579, 1124, 1235, 2170
EcoT22I ATGCAT 2 cut(s) 452, 732
EcoT38I GRGCYC 3 cut(s) 315, 1235, 1486
ErhI CCWWGG 5 cut(s) 85, 579, 1124, 1235, 2170
Esp3I CGTCTC 2 cut(s) 128, 2106
FalI AAGNNNNNCTT 2 cut(s) 551, 583
FauI CCCGC 3 cut(s) 243, 247, 1946
FokI GGATG 7 cut(s) 208, 259, 412, 786, 1166, 1237, 1340
FriOI GRGCYC 3 cut(s) 315, 1235, 1486
FspAI RTGCGCAY 1 cut(s) 1821
FspBI CTAG 6 cut(s) 410, 966, 1010, 1698, 1803, 1967
FspI TGCGCA 3 cut(s) 156, 647, 1821
GlaI GCGC 7 cut(s) 143, 156, 593, 647, 675, 1671, 1821
GsuI CTGGAG 4 cut(s) 492, 516, 534, 1968
HaeII RGCGCY 1 cut(s) 1673
HaeIII GGCC 5 cut(s) 285, 533, 1207, 1233, 1957
HapII CCGG 7 cut(s) 131, 193, 257, 530, 1305, 1398, 1437
HgaI GACGC 1 cut(s) 640
HhaI GCGC 7 cut(s) 144, 157, 594, 648, 676, 1672, 1822
Hin6I GCGC 7 cut(s) 142, 155, 592, 646, 674, 1670, 1820
HinP1I GCGC 7 cut(s) 142, 155, 592, 646, 674, 1670, 1820
HinfI GANTC 6 cut(s) 62, 81, 526, 923, 1887, 2017
HpaII CCGG 7 cut(s) 131, 193, 257, 530, 1305, 1398, 1437
HphI GGTGA 4 cut(s) 595, 998, 1202, 1673
Hpy188I TCNGA 5 cut(s) 40, 227, 355, 841, 1996
Hpy99I CGWCG 1 cut(s) 1982
HpyAV CCTTC 4 cut(s) 190, 593, 2044, 2130
HpyCH4III ACNGT 4 cut(s) 403, 1018, 1511, 1826
HpyCH4IV ACGT 5 cut(s) 137, 159, 457, 1194, 1728
HpyF10VI GCNNNNNNNGC 5 cut(s) 24, 680, 1160, 1274, 1963
HpyF3I CTNAG 6 cut(s) 164, 384, 1563, 1959, 1993, 2178
HpySE526I ACGT 5 cut(s) 137, 159, 457, 1194, 1728
HspAI GCGC 7 cut(s) 142, 155, 592, 646, 674, 1670, 1820
Kzo9I GATC 9 cut(s) 57, 334, 610, 865, 1108, 1289, 1301, 1430, 1675
LmnI GCTCC 6 cut(s) 119, 515, 564, 811, 1265, 1691
LweI GCATC 7 cut(s) 237, 434, 717, 1150, 1318, 1454, 1866
MaeI CTAG 6 cut(s) 410, 966, 1010, 1698, 1803, 1967
MaeII ACGT 5 cut(s) 137, 159, 457, 1194, 1728
MaeIII GTNAC 7 cut(s) 28, 986, 1169, 1190, 1336, 1540, 1808
MalI GATC 9 cut(s) 59, 336, 612, 867, 1110, 1291, 1303, 1432, 1677
MboI GATC 9 cut(s) 57, 334, 610, 865, 1108, 1289, 1301, 1430, 1675
MboII GAAGA 4 cut(s) 88, 1078, 2065, 2096
MfeI CAATTG 1 cut(s) 714
MflI RGATCY 6 cut(s) 334, 865, 1108, 1289, 1301, 1430
MhlI GDGCHC 3 cut(s) 315, 1235, 1486
MlsI TGGCCA 1 cut(s) 285
MluNI TGGCCA 1 cut(s) 285
MlyI GAGTC 2 cut(s) 75, 932
MmeI TCCRAC 1 cut(s) 63
Mox20I TGGCCA 1 cut(s) 285
Mph1103I ATGCAT 2 cut(s) 452, 732
MscI TGGCCA 1 cut(s) 285
MseI TTAA 5 cut(s) 636, 831, 1259, 1769, 2060
MslI CAYNNNNRTG 3 cut(s) 735, 1782, 1827
Msp20I TGGCCA 1 cut(s) 285
MspA1I CMGCKG 1 cut(s) 1925
MspI CCGG 7 cut(s) 131, 193, 257, 530, 1305, 1398, 1437
MspR9I CCNGG 9 cut(s) 20, 244, 257, 495, 513, 863, 997, 1165, 1653
MunI CAATTG 1 cut(s) 714
Mva1269I GAATGC 2 cut(s) 1717, 1749
MvaI CCWGG 8 cut(s) 20, 244, 495, 513, 863, 997, 1165, 1653
MvnI CGCG 2 cut(s) 594, 879
MwoI GCNNNNNNNGC 5 cut(s) 24, 680, 1160, 1274, 1963
NciI CCSGG 1 cut(s) 257
NcoI CCATGG 3 cut(s) 85, 579, 1124
NdeII GATC 9 cut(s) 57, 334, 610, 865, 1108, 1289, 1301, 1430, 1675
NmuCI GTSAC 3 cut(s) 986, 1169, 1190
NsbI TGCGCA 3 cut(s) 156, 647, 1821
NsiI ATGCAT 2 cut(s) 452, 732
NspI RCATGY 2 cut(s) 674, 734
OliI CACNNNNGTG 1 cut(s) 1827
PaeR7I CTCGAG 1 cut(s) 1916
PcsI WCGNNNNNNNCGW 1 cut(s) 71
PctI GAATGC 2 cut(s) 1717, 1749
PfeI GAWTC 4 cut(s) 62, 526, 1887, 2017
PflMI CCANNNNNTGG 1 cut(s) 1658
PfoI TCCNGGA 2 cut(s) 493, 511
PleI GAGTC 2 cut(s) 75, 931
PpsI GAGTC 2 cut(s) 75, 931
Ppu21I YACGTR 1 cut(s) 458
PpuMI RGGWCCY 1 cut(s) 2174
PshAI GACNNNNGTC 1 cut(s) 2172
Psp124BI GAGCTC 1 cut(s) 315
Psp5II RGGWCCY 1 cut(s) 2174
Psp6I CCWGG 8 cut(s) 18, 242, 493, 511, 861, 995, 1163, 1651
PspGI CCWGG 8 cut(s) 18, 242, 493, 511, 861, 995, 1163, 1651
PspOMI GGGCCC 1 cut(s) 1231
PspPI GGNCC 7 cut(s) 227, 1128, 1206, 1231, 1232, 1955, 2174
PspPPI RGGWCCY 1 cut(s) 2174
PstI CTGCAG 5 cut(s) 268, 1159, 1604, 2011, 2154
PsuI RGATCY 6 cut(s) 334, 865, 1108, 1289, 1301, 1430
PvuII CAGCTG 1 cut(s) 1925
RsaI GTAC 6 cut(s) 203, 405, 1384, 1508, 1519, 1702
RsaNI GTAC 6 cut(s) 202, 404, 1383, 1507, 1518, 1701
RseI CAYNNNNRTG 3 cut(s) 735, 1782, 1827
SacI GAGCTC 1 cut(s) 315
SaqAI TTAA 5 cut(s) 636, 831, 1259, 1769, 2060
Sau3AI GATC 9 cut(s) 57, 334, 610, 865, 1108, 1289, 1301, 1430, 1675
Sau96I GGNCC 7 cut(s) 227, 1128, 1206, 1231, 1232, 1955, 2174
ScaI AGTACT 1 cut(s) 1508
SchI GAGTC 2 cut(s) 75, 932
ScrFI CCNGG 9 cut(s) 20, 244, 257, 495, 513, 863, 997, 1165, 1653
SduI GDGCHC 3 cut(s) 315, 1235, 1486
SfaNI GCATC 7 cut(s) 237, 434, 717, 1150, 1318, 1454, 1866
SfcI CTRYAG 6 cut(s) 264, 1050, 1155, 1600, 2007, 2150
Sfr274I CTCGAG 1 cut(s) 1916
SgrAI CRCCGGYG 1 cut(s) 1436
SinI GGWCC 3 cut(s) 227, 1128, 2174
SlaI CTCGAG 1 cut(s) 1916
SmiMI CAYNNNNRTG 3 cut(s) 735, 1782, 1827
SmlI CTYRAG 2 cut(s) 293, 1916
SmoI CTYRAG 2 cut(s) 293, 1916
SsiI CCGC 7 cut(s) 123, 236, 240, 879, 1940, 1953, 2146
SspI AATATT 2 cut(s) 1081, 1849
SspMI CTAG 6 cut(s) 410, 966, 1010, 1698, 1803, 1967
SstI GAGCTC 1 cut(s) 315
StyD4I CCNGG 9 cut(s) 18, 242, 255, 493, 511, 861, 995, 1163, 1651
StyI CCWWGG 5 cut(s) 85, 579, 1124, 1235, 2170
TaaI ACNGT 4 cut(s) 403, 1018, 1511, 1826
TaiI ACGT 5 cut(s) 140, 162, 460, 1197, 1731
TaqI TCGA 4 cut(s) 45, 60, 1917, 2166
TaqII GACCGA 1 cut(s) 1359
TatI WGTACW 5 cut(s) 201, 403, 1382, 1506, 1700
TauI GCSGC 4 cut(s) 125, 882, 1942, 2149
TfiI GAWTC 4 cut(s) 62, 526, 1887, 2017
Tru1I TTAA 5 cut(s) 636, 831, 1259, 1769, 2060
Tru9I TTAA 5 cut(s) 636, 831, 1259, 1769, 2060
TscAI CASTG 2 cut(s) 157, 690
TseFI GTSAC 3 cut(s) 986, 1169, 1190
Tsp45I GTSAC 3 cut(s) 986, 1169, 1190
TspDTI ATGAA 5 cut(s) 434, 612, 1053, 1242, 1859
TspGWI ACGGA 4 cut(s) 132, 1172, 1810, 2055
TspRI CASTG 2 cut(s) 157, 690
Van91I CCANNNNNTGG 1 cut(s) 1658
VpaK11BI GGWCC 3 cut(s) 227, 1128, 2174
XapI RAATTY 4 cut(s) 365, 463, 1040, 1387
XbaI TCTAGA 2 cut(s) 1009, 1802
XceI RCATGY 2 cut(s) 674, 734
XhoI CTCGAG 1 cut(s) 1916
XspI CTAG 6 cut(s) 410, 966, 1010, 1698, 1803, 1967
ZrmI AGTACT 1 cut(s) 1508
Zsp2I ATGCAT 2 cut(s) 452, 732
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.