Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0010059

Physical Location & Seq

Genomic Coordinates Forward (+)

64248796 .. 64254351

Gene Structure 5556 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000004993

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2178 bp

ATGTCGTACAAGAGCCTCGACGCCGTTACGGTCGCCGACATCGGAGCTCTCGGGATTGAGCCGGAGCTCGCCAACAGTCTCCATGGAAGTCTCGCTAAGATCATCGCCGATCACGGCGCTGCCACTCAGGACACGTGGCGCCGTATCACTGGGCACGTGCTCAGCCCGGACCTCCCCTTCTCCTTCCACCAGATGATGTACTACGGTTGCTATAAGGACTTCGGAACCGACCCGCCCGCCTGGCTCCCCGACCCGGAGACTGCGGTTTTAACAAATGCCGGCCAGCTACTGGAGAGGCGGGGAAAAGAGTTTTTGGGTTCTCGATATAAGGATCCCATATCTAGCTTTTCCGATTTTCAGGAGTTTTCGGTGTCAAACCCAGAGGTTTATTGGAAAACTGTACTAGATGAGATGAATGTGTCGTTTTACAAGCCTCCCCAATGTATTTTACGTGATAATCTGTCTGGAGATGGGCATTTATCAGTTCCAGGAGTTCAATGGCTTCCTGGAGCATGTGTGAATCCAGCAAAGAATTGCTTGAGTTTAAATTGTAAGAGGAGCTTGAATGATACAATGGTAGTATGGCGTGACGAAGGAAAGGATGATCTGCCTTTGAATAAGATGACACTTAAAGAATTGCGTGCAGAGGTTTGGTTAGTTGCACATGCTCTCCAAGCACTAGGTCTGGAGAAAGGATCTGCAATTGCAATCGATATGCCAATGAATGTCATTTCTGTAGTTATCTACCTGGCTATTGTTCTGGCAGGTTACGTAGTTGTATCAATCGCTGATAGTTTTGCTCCCCCTGAAATATCAACCAGACTAAAAATTTCGGAGGCAAAAGCCATATTTACCCAGGATGTAATAGTTCGTGGTGAGAAAAGTCTACCCTTGTATAGTAAAATCGTGGATGCTCAATCTCCTATGGCCATTGTTCTCCTTACAAGGGGCTCCAACTCTAGTGTGAAATTGCGTGATGGTGACATTTCTTGGCATGATTTTCTAGAAACAGTAAAAGATTTCAAAGATGAATTTGCTGCTGTGGAACAACCTGTAGAAGCATTTACAAATATCCTCTTCTCATCTGGAACTACTGGGGACCCAAAGGCAATTCCATGGACCCATTCAACTCCTTTCAAAGCAGCTGCAGATGCCTGGTGCCACATGGACATTCGTAAAGGTGATGTTATTGCTTGGCCCACAAATCTTGGGTGGATGATGGGCCCTTGGTTGGTATATGCTTCTTTGTTAAATGGTGCTTCCATGGCCCTATATAATGGATCTCCACTTGGACCTGGCTTTTCCAAGTTTGTTCAGGATGCCAAAGTAACAATGCTTGGCGTAATCCCTAGTATTGTTCGGACATGGAAAAGTACAAATTCTACTTCAGGCTATGATTGGTCAGCCATCCGTTGCTTTAGCTCCACCGGTGAAGCATCAAATGTAGATGAATACCTATGGCTGATGGGGAGGGCTGGCTACAAGCCTATCATAGAGTATTGTGGTGGTACAGAAATTGGCGGTGCCTTTGTTTCTGGGTCATTGTTGCAGGCTCAGTCTTTGGCTTCTTTTAGCACTCCAGCTATGGGCTGCAGTTTGTTTATTCTTGGCACTGATGGAAGTCCTATTCCACAAAATGAACCAGGGGTTGGTGAACTGGCGCTTGGTCCCCTCATGTTTGGAGCTTCGAGTACACTGTTGAATGCTGATCATTATGAGGTCTATTTTAAGGGAATGCCATTATGGAATGGGAAGGTTTTAAGAAGACATGGGGATTTGTTGGAGCGGACATCCAGGGGTTACTATCATGCTCATGGTCGAGCAGATGATACAATGAATCTTGGAGGCATCAAGGTTAGTTCTGTTGAGATTGAACGAATCTGTAATGCAATTGACACCAACATCCTCGAGACAGCTGCCATTGGGGTGCCGCCTGCTGGAGGCGGACCGGAGCAGCTGGTAATTGCTGTTGTATTTAAGAATTCAGATAATCCACCTGCAGATTTGAATCAACTAAGAGCATCCTTCAATTCAGCTGTGCAGAAGAAACTAAATCCCTTGTTTAAGGTTTCTAGAGTTGTGCCTCTCCCCTCTCTTCCAAGAACAGCAACAAATAAGGTCATGAGAAGGATTCTGCGGCAGCAGTTTGGTCAACTCGACCAAGGTGCTAAGCTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ACAS_N AMP-binding AMP-binding_C

Protein Analysis

726

Amino Acids

78.99

Weight (kDa)

5.96

Isoelectric Point (pI)

34.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AMP-binding	PF00501	205 - 554	1.2e-44	AMP-binding enzyme

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010059)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G23050 AT5G23050
fragaria_vesca	FvH4_5g12960 FvH4_5g12960
malus_domestica	MD14G1218900.v1.1
prunus_persica	Prupe.5G213100_v2.0.a1 Prupe.5G213100_v2.0.a1
pyrus_communis	pycom14g18140
rosa_chinensis	RchiOBHm_Chr7g0184211
rosa_laevigata	RLG00000004993
rosa_multiflora	Rmu_sc0013812.1_g000002
rosa_roxburghii	Rroxscaffold_3G00269800
rosa_rugosa	Rorug06G0463800 Rorug06G0463800 Rorug06G0463900 Rorug06G0463900
rosa_samantha	Rh7AG081900 Rh7BG065300 Rh7CG065700 Rh7DG065400
rosa_wichuraiana	Rw7G005360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2005
Acc36I	ACCTGC	2 cut(s)	755, 2005
AccB1I	GGYRCC	4 cut(s)	138, 1158, 1523, 1927
AccB7I	CCANNNNNTGG	2 cut(s)	289, 1649
AccBSI	CCGCTC	1 cut(s)	1786
AccI	GTMKAC	1 cut(s)	886
AciI	CCGC	9 cut(s)	233, 237, 263, 298, 1521, 1786, 1931, 1944, 2137
AclWI	GGATC	4 cut(s)	326, 339, 703, 1288
AcoI	YGGCCR	2 cut(s)	280, 927
AcsI	RAATTY	4 cut(s)	828, 1031, 1378, 1981
AcuI	CTGAAG	1 cut(s)	1371
AcvI	CACGTG	2 cut(s)	135, 157
AcyI	GRCGYC	2 cut(s)	21, 139
AfaI	GTAC	6 cut(s)	8, 200, 402, 1375, 1510, 1693
AfiI	CCNNNNNNNGG	8 cut(s)	253, 289, 946, 1231, 1586, 1649, 1937, 1940
AflIII	ACRYGT	1 cut(s)	132
AgeI	ACCGGT	1 cut(s)	1427
AjnI	CCWGG	9 cut(s)	239, 487, 505, 747, 855, 1154, 1294, 1642, 1793
AjuI	GAANNNNNNNTTGG	4 cut(s)	1632, 1647, 1664, 1679
Alw21I	GWGCWC	3 cut(s)	49, 69, 162
Alw26I	GTCTC	4 cut(s)	83, 95, 251, 1904
AlwI	GGATC	4 cut(s)	326, 339, 703, 1288
AlwNI	CAGNNNCTG	1 cut(s)	289
Ama87I	CYCGRG	2 cut(s)	50, 1907
AoxI	GGCC	5 cut(s)	280, 927, 1196, 1222, 1266
ApaI	GGGCCC	1 cut(s)	1226
ApeKI	GCWGC	8 cut(s)	119, 1037, 1142, 1145, 1590, 1916, 1954, 2140
ApoI	RAATTY	4 cut(s)	828, 1031, 1378, 1981
AsiGI	ACCGGT	1 cut(s)	1427
AspLEI	GCGC	3 cut(s)	119, 141, 1663
AsuC2I	CCSGG	2 cut(s)	167, 254
AsuHPI	GGTGA	5 cut(s)	887, 992, 1193, 1442, 1664
AvaI	CYCGRG	2 cut(s)	50, 1907
AvaII	GGWCC	6 cut(s)	169, 1099, 1119, 1292, 1667, 1946
BaeGI	GKGCMC	2 cut(s)	156, 1226
BaeI	ACNNNNGTAYC	2 cut(s)	561, 594
BalI	TGGCCA	1 cut(s)	929
BamHI	GGATCC	1 cut(s)	331
BanI	GGYRCC	4 cut(s)	138, 1158, 1523, 1927
BanII	GRGCYC	4 cut(s)	49, 69, 953, 1226
BbrPI	CACGTG	2 cut(s)	135, 157
BbsI	GAAGAC	1 cut(s)	1771
Bbv12I	GWGCWC	3 cut(s)	49, 69, 162
BbvI	GCAGC	8 cut(s)	106, 1024, 1132, 1154, 1577, 1903, 1966, 2152
BccI	CCATC	6 cut(s)	464, 971, 1213, 1415, 1459, 1610
BceAI	ACGGC	3 cut(s)	8, 126, 130
BcgI	CGANNNNNNTGC	3 cut(s)	1898, 1932, 2147
BciT130I	CCWGG	9 cut(s)	241, 489, 507, 749, 857, 1156, 1296, 1644, 1795
BclI	TGATCA	1 cut(s)	1708
BcnI	CCSGG	2 cut(s)	167, 254
BcoDI	GTCTC	4 cut(s)	83, 95, 251, 1904
BfaI	CTAG	7 cut(s)	342, 404, 680, 960, 1004, 1350, 2073
BfmI	CTRYAG	5 cut(s)	735, 1053, 1146, 1591, 1998
BfoI	RGCGCY	3 cut(s)	120, 142, 1664
BfuAI	ACCTGC	2 cut(s)	755, 2005
BglI	GCCNNNNNGGC	1 cut(s)	241
BlpI	GCTNAGC	2 cut(s)	161, 2169
Bme18I	GGWCC	6 cut(s)	169, 1099, 1119, 1292, 1667, 1946
BmeT110I	CYCGRG	2 cut(s)	50, 1907
BmrI	ACTGGG	2 cut(s)	159, 1104
BmsI	GCATC	6 cut(s)	901, 1141, 1309, 1445, 1857, 2030
BmuI	ACTGGG	2 cut(s)	159, 1104
BpiI	GAAGAC	1 cut(s)	1771
BpmI	CTGGAG	6 cut(s)	311, 486, 528, 707, 1563, 1959
Bpu1102I	GCTNAGC	2 cut(s)	161, 2169
BpuEI	CTTGAG	1 cut(s)	559
BpuMI	CCSGG	2 cut(s)	167, 254
Bsa29I	ATCGAT	1 cut(s)	711
BsaAI	YACGTR	4 cut(s)	135, 157, 452, 772
BsaBI	GATNNNNATC	1 cut(s)	2007
BsaHI	GRCGYC	2 cut(s)	21, 139
BsaJI	CCNNGG	8 cut(s)	82, 855, 1115, 1226, 1263, 1643, 1794, 2161
BsaWI	WCCGGW	2 cut(s)	1427, 1948
BsaXI	ACNNNNNCTCC	4 cut(s)	501, 531, 654, 684
Bsc4I	CCNNNNNNNGG	8 cut(s)	253, 289, 946, 1231, 1586, 1649, 1937, 1940
Bse118I	RCCGGY	2 cut(s)	278, 1427
Bse1I	ACTGG	4 cut(s)	154, 294, 1099, 1662
Bse8I	GATNNNNATC	1 cut(s)	2007
BseBI	CCWGG	9 cut(s)	241, 489, 507, 749, 857, 1156, 1296, 1644, 1795
BseCI	ATCGAT	1 cut(s)	711
BseDI	CCNNGG	8 cut(s)	82, 855, 1115, 1226, 1263, 1643, 1794, 2161
BseGI	GGATG	9 cut(s)	607, 865, 916, 1221, 1324, 1407, 1790, 1902, 2021
BseJI	GATNNNNATC	1 cut(s)	2007
BseLI	CCNNNNNNNGG	8 cut(s)	253, 289, 946, 1231, 1586, 1649, 1937, 1940
BseMII	CTCAG	3 cut(s)	140, 175, 1568
BseNI	ACTGG	4 cut(s)	154, 294, 1099, 1662
BseRI	GAGGAG	1 cut(s)	571
BseSI	GKGCMC	2 cut(s)	156, 1226
BseXI	GCAGC	8 cut(s)	106, 1024, 1132, 1154, 1577, 1903, 1966, 2152
BsgI	GTGCAG	2 cut(s)	663, 2060
Bsh1285I	CGRYCG	1 cut(s)	33
BshFI	GGCC	5 cut(s)	282, 929, 1198, 1224, 1268
BshNI	GGYRCC	4 cut(s)	138, 1158, 1523, 1927
BshTI	ACCGGT	1 cut(s)	1427
BshVI	ATCGAT	1 cut(s)	711
BsiEI	CGRYCG	1 cut(s)	33
BsiHKAI	GWGCWC	3 cut(s)	49, 69, 162
BsiHKCI	CYCGRG	2 cut(s)	50, 1907
BsiSI	CCGG	6 cut(s)	62, 167, 254, 279, 1428, 1949
BslFI	GGGAC	2 cut(s)	1112, 1653
BslI	CCNNNNNNNGG	8 cut(s)	253, 289, 946, 1231, 1586, 1649, 1937, 1940
BsmAI	GTCTC	4 cut(s)	83, 95, 251, 1904
BsmFI	GGGAC	2 cut(s)	1112, 1653
BsmI	GAATGC	2 cut(s)	1708, 1740
BsnI	GGCC	5 cut(s)	282, 929, 1198, 1224, 1268
BsoBI	CYCGRG	2 cut(s)	50, 1907
Bsp120I	GGGCCC	1 cut(s)	1222
Bsp1286I	GDGCHC	6 cut(s)	49, 69, 156, 162, 953, 1226
Bsp143I	GATC	7 cut(s)	99, 109, 331, 604, 695, 1280, 1708
Bsp1720I	GCTNAGC	2 cut(s)	161, 2169
Bsp19I	CCATGG	3 cut(s)	82, 1115, 1263
BspACI	CCGC	9 cut(s)	233, 237, 263, 298, 1521, 1786, 1931, 1944, 2137
BspANI	GGCC	5 cut(s)	282, 929, 1198, 1224, 1268
BspCNI	CTCAG	3 cut(s)	139, 174, 1567
BspDI	ATCGAT	1 cut(s)	711
BspHI	TCATGA	1 cut(s)	2121
BspMAI	CTGCAG	3 cut(s)	1150, 1595, 2002
BspMI	ACCTGC	2 cut(s)	755, 2005
BspPI	GGATC	4 cut(s)	326, 339, 703, 1288
BspT107I	GGYRCC	4 cut(s)	138, 1158, 1523, 1927
BsrBI	CCGCTC	1 cut(s)	1786
BsrFI	RCCGGY	2 cut(s)	278, 1427
BsrI	ACTGG	4 cut(s)	154, 294, 1099, 1662
BssAI	RCCGGY	2 cut(s)	278, 1427
BssECI	CCNNGG	8 cut(s)	82, 855, 1115, 1226, 1263, 1643, 1794, 2161
BssMI	GATC	7 cut(s)	99, 109, 331, 604, 695, 1280, 1708
BssNI	GRCGYC	2 cut(s)	21, 139
BssT1I	CCWWGG	5 cut(s)	82, 1115, 1226, 1263, 2161
Bst2UI	CCWGG	9 cut(s)	241, 489, 507, 749, 857, 1156, 1296, 1644, 1795
Bst4CI	ACNGT	6 cut(s)	31, 77, 206, 400, 1012, 1698
Bst6I	CTCTTC	2 cut(s)	1082, 2100
BstACI	GRCGYC	2 cut(s)	21, 139
BstBAI	YACGTR	4 cut(s)	135, 157, 452, 772
BstC8I	GCNNGC	8 cut(s)	69, 237, 280, 284, 642, 1477, 1551, 1935
BstDEI	CTNAG	6 cut(s)	96, 126, 161, 1554, 2015, 2169
BstDSI	CCRYGG	3 cut(s)	82, 1115, 1263
BstENI	CCTNNNNNAGG	1 cut(s)	1938
BstF5I	GGATG	9 cut(s)	607, 865, 916, 1221, 1324, 1407, 1790, 1902, 2021
BstH2I	RGCGCY	3 cut(s)	120, 142, 1664
BstHHI	GCGC	3 cut(s)	119, 141, 1663
BstKTI	GATC	7 cut(s)	102, 112, 334, 607, 698, 1283, 1711
BstMAI	GTCTC	4 cut(s)	83, 95, 251, 1904
BstMBI	GATC	7 cut(s)	99, 109, 331, 604, 695, 1280, 1708
BstMCI	CGRYCG	1 cut(s)	33
BstMWI	GCNNNNNNNGC	4 cut(s)	241, 674, 1151, 1265
BstNI	CCWGG	9 cut(s)	241, 489, 507, 749, 857, 1156, 1296, 1644, 1795
BstNSI	RCATGY	2 cut(s)	516, 668
BstSFI	CTRYAG	5 cut(s)	735, 1053, 1146, 1591, 1998
BstSLI	GKGCMC	2 cut(s)	156, 1226
BstSNI	TACGTA	1 cut(s)	772
BstV1I	GCAGC	8 cut(s)	106, 1024, 1132, 1154, 1577, 1903, 1966, 2152
BstV2I	GAAGAC	1 cut(s)	1771
BstX2I	RGATCY	3 cut(s)	331, 695, 1280
BstYI	RGATCY	3 cut(s)	331, 695, 1280
Bsu15I	ATCGAT	1 cut(s)	711
BsuRI	GGCC	5 cut(s)	282, 929, 1198, 1224, 1268
BsuTUI	ATCGAT	1 cut(s)	711
BtgI	CCRYGG	3 cut(s)	82, 1115, 1263
BtgZI	GCGATG	1 cut(s)	88
BtsCI	GGATG	9 cut(s)	607, 865, 916, 1221, 1324, 1407, 1790, 1902, 2021
BtsIMutI	CAGTG	3 cut(s)	147, 1611, 1694
BveI	ACCTGC	2 cut(s)	755, 2005
Cac8I	GCNNGC	8 cut(s)	69, 237, 280, 284, 642, 1477, 1551, 1935
CaiI	CAGNNNCTG	1 cut(s)	289
CciI	TCATGA	1 cut(s)	2121
CfoI	GCGC	3 cut(s)	119, 141, 1663
Cfr10I	RCCGGY	2 cut(s)	278, 1427
ClaI	ATCGAT	1 cut(s)	711
CpoI	CGGWCCG	1 cut(s)	1946
CseI	GACGC	1 cut(s)	29
Csp6I	GTAC	6 cut(s)	7, 199, 401, 1374, 1509, 1692
CspAI	ACCGGT	1 cut(s)	1427
CspCI	CAANNNNNGTGG	2 cut(s)	1189, 1224
CspI	CGGWCCG	1 cut(s)	1946
CviQI	GTAC	6 cut(s)	7, 199, 401, 1374, 1509, 1692
DdeI	CTNAG	6 cut(s)	96, 126, 161, 1554, 2015, 2169
DinI	GGCGCC	1 cut(s)	140
DpnI	GATC	7 cut(s)	101, 111, 333, 606, 697, 1282, 1710
DpnII	GATC	7 cut(s)	99, 109, 331, 604, 695, 1280, 1708
DraI	TTTAAA	1 cut(s)	546
EaeI	YGGCCR	2 cut(s)	280, 927
Eam1104I	CTCTTC	2 cut(s)	1082, 2100
EarI	CTCTTC	2 cut(s)	1082, 2100
EciI	GGCGGA	1 cut(s)	1959
Ecl136II	GAGCTC	2 cut(s)	47, 67
Eco105I	TACGTA	1 cut(s)	772
Eco130I	CCWWGG	5 cut(s)	82, 1115, 1226, 1263, 2161
Eco24I	GRGCYC	4 cut(s)	49, 69, 953, 1226
Eco47I	GGWCC	6 cut(s)	169, 1099, 1119, 1292, 1667, 1946
Eco53kI	GAGCTC	2 cut(s)	47, 67
Eco57I	CTGAAG	1 cut(s)	1371
Eco72I	CACGTG	2 cut(s)	135, 157
Eco88I	CYCGRG	2 cut(s)	50, 1907
EcoICRI	GAGCTC	2 cut(s)	47, 67
EcoNI	CCTNNNNNAGG	1 cut(s)	1938
EcoO109I	RGGNCCY	2 cut(s)	1099, 1223
EcoRI	GAATTC	1 cut(s)	1981
EcoRII	CCWGG	9 cut(s)	239, 487, 505, 747, 855, 1154, 1294, 1642, 1793
EcoT14I	CCWWGG	5 cut(s)	82, 1115, 1226, 1263, 2161
EcoT38I	GRGCYC	4 cut(s)	49, 69, 953, 1226
EgeI	GGCGCC	1 cut(s)	140
EheI	GGCGCC	1 cut(s)	140
ErhI	CCWWGG	5 cut(s)	82, 1115, 1226, 1263, 2161
FalI	AAGNNNNNCTT	4 cut(s)	521, 553, 545, 577
FaqI	GGGAC	2 cut(s)	1112, 1653
FauI	CCCGC	3 cut(s)	240, 244, 291
FbaI	TGATCA	1 cut(s)	1708
FblI	GTMKAC	1 cut(s)	886
FokI	GGATG	9 cut(s)	614, 872, 923, 1228, 1331, 1394, 1777, 1889, 2008
FriOI	GRGCYC	4 cut(s)	49, 69, 953, 1226
FspBI	CTAG	7 cut(s)	342, 404, 680, 960, 1004, 1350, 2073
GlaI	GCGC	3 cut(s)	118, 140, 1662
GsuI	CTGGAG	6 cut(s)	311, 486, 528, 707, 1563, 1959
HaeII	RGCGCY	3 cut(s)	120, 142, 1664
HaeIII	GGCC	5 cut(s)	282, 929, 1198, 1224, 1268
HapII	CCGG	6 cut(s)	62, 167, 254, 279, 1428, 1949
HgaI	GACGC	1 cut(s)	29
HhaI	GCGC	3 cut(s)	119, 141, 1663
Hin1I	GRCGYC	2 cut(s)	21, 139
Hin6I	GCGC	3 cut(s)	117, 139, 1661
HinP1I	GCGC	3 cut(s)	117, 139, 1661
HincII	GTYRAC	1 cut(s)	2153
HindII	GTYRAC	1 cut(s)	2153
HinfI	GANTC	5 cut(s)	520, 1837, 1878, 2008, 2131
HpaII	CCGG	6 cut(s)	62, 167, 254, 279, 1428, 1949
HphI	GGTGA	5 cut(s)	887, 992, 1193, 1442, 1664
Hpy166II	GTNNAC	4 cut(s)	887, 1655, 1694, 2153
Hpy188I	TCNGA	6 cut(s)	44, 224, 352, 835, 1362, 1987
Hpy8I	GTNNAC	4 cut(s)	887, 1655, 1694, 2153
Hpy99I	CGWCG	1 cut(s)	23
HpyAV	CCTTC	6 cut(s)	187, 193, 587, 1747, 2035, 2121
HpyCH4III	ACNGT	6 cut(s)	31, 77, 206, 400, 1012, 1698
HpyCH4IV	ACGT	4 cut(s)	134, 156, 451, 771
HpyF10VI	GCNNNNNNNGC	4 cut(s)	241, 674, 1151, 1265
HpyF3I	CTNAG	6 cut(s)	96, 126, 161, 1554, 2015, 2169
HpySE526I	ACGT	4 cut(s)	134, 156, 451, 771
Hsp92I	GRCGYC	2 cut(s)	21, 139
HspAI	GCGC	3 cut(s)	117, 139, 1661
KasI	GGCGCC	1 cut(s)	138
KflI	GGGWCCC	1 cut(s)	1099
KroI	GCCGGC	1 cut(s)	278
KroNI	GCCGGC	1 cut(s)	280
Ksp22I	TGATCA	1 cut(s)	1708
Kzo9I	GATC	7 cut(s)	99, 109, 331, 604, 695, 1280, 1708
Lsp1109I	GCAGC	8 cut(s)	106, 1024, 1132, 1154, 1577, 1903, 1966, 2152
LweI	GCATC	6 cut(s)	901, 1141, 1309, 1445, 1857, 2030
MaeI	CTAG	7 cut(s)	342, 404, 680, 960, 1004, 1350, 2073
MaeII	ACGT	4 cut(s)	134, 156, 451, 771
MaeIII	GTNAC	6 cut(s)	25, 587, 767, 980, 1327, 1799
MalI	GATC	7 cut(s)	101, 111, 333, 606, 697, 1282, 1710
MbiI	CCGCTC	1 cut(s)	1786
MboI	GATC	7 cut(s)	99, 109, 331, 604, 695, 1280, 1708
MboII	GAAGA	4 cut(s)	1069, 1776, 2056, 2087
MfeI	CAATTG	2 cut(s)	702, 1890
MflI	RGATCY	3 cut(s)	331, 695, 1280
MhlI	GDGCHC	6 cut(s)	49, 69, 156, 162, 953, 1226
MlsI	TGGCCA	1 cut(s)	929
MluNI	TGGCCA	1 cut(s)	929
Mly113I	GGCGCC	1 cut(s)	139
MmeI	TCCRAC	2 cut(s)	978, 1761
Mox20I	TGGCCA	1 cut(s)	929
MroNI	GCCGGC	1 cut(s)	278
MscI	TGGCCA	1 cut(s)	929
MseI	TTAA	8 cut(s)	269, 545, 630, 1250, 1728, 1760, 1977, 2064
MslI	CAYNNNNRTG	2 cut(s)	1812, 1925
Msp20I	TGGCCA	1 cut(s)	929
MspA1I	CMGCKG	4 cut(s)	1145, 1916, 1957, 2036
MspI	CCGG	6 cut(s)	62, 167, 254, 279, 1428, 1949
MunI	CAATTG	2 cut(s)	702, 1890
Mva1269I	GAATGC	2 cut(s)	1708, 1740
MvaI	CCWGG	9 cut(s)	241, 489, 507, 749, 857, 1156, 1296, 1644, 1795
MwoI	GCNNNNNNNGC	4 cut(s)	241, 674, 1151, 1265
NaeI	GCCGGC	1 cut(s)	280
NarI	GGCGCC	1 cut(s)	139
NciI	CCSGG	2 cut(s)	167, 254
NcoI	CCATGG	3 cut(s)	82, 1115, 1263
NdeII	GATC	7 cut(s)	99, 109, 331, 604, 695, 1280, 1708
NgoMIV	GCCGGC	1 cut(s)	278
NmuCI	GTSAC	2 cut(s)	587, 980
NspI	RCATGY	2 cut(s)	516, 668
PaeR7I	CTCGAG	1 cut(s)	1907
PagI	TCATGA	1 cut(s)	2121
PaqCI	CACCTGC	1 cut(s)	2005
PctI	GAATGC	2 cut(s)	1708, 1740
PdiI	GCCGGC	1 cut(s)	280
PfeI	GAWTC	5 cut(s)	520, 1837, 1878, 2008, 2131
PflMI	CCANNNNNTGG	2 cut(s)	289, 1649
PfoI	TCCNGGA	2 cut(s)	487, 505
PinAI	ACCGGT	1 cut(s)	1427
PluTI	GGCGCC	1 cut(s)	142
PmaCI	CACGTG	2 cut(s)	135, 157
PmlI	CACGTG	2 cut(s)	135, 157
Ppu21I	YACGTR	4 cut(s)	135, 157, 452, 772
PpuMI	RGGWCCY	1 cut(s)	1099
Psp124BI	GAGCTC	2 cut(s)	49, 69
Psp5II	RGGWCCY	1 cut(s)	1099
Psp6I	CCWGG	9 cut(s)	239, 487, 505, 747, 855, 1154, 1294, 1642, 1793
PspCI	CACGTG	2 cut(s)	135, 157
PspGI	CCWGG	9 cut(s)	239, 487, 505, 747, 855, 1154, 1294, 1642, 1793
PspOMI	GGGCCC	1 cut(s)	1222
PspPPI	RGGWCCY	1 cut(s)	1099
PstI	CTGCAG	3 cut(s)	1150, 1595, 2002
PstNI	CAGNNNCTG	1 cut(s)	289
PsuI	RGATCY	3 cut(s)	331, 695, 1280
PvuII	CAGCTG	4 cut(s)	1145, 1916, 1957, 2036
RsaI	GTAC	6 cut(s)	8, 200, 402, 1375, 1510, 1693
RsaNI	GTAC	6 cut(s)	7, 199, 401, 1374, 1509, 1692
RseI	CAYNNNNRTG	2 cut(s)	1812, 1925
Rsr2I	CGGWCCG	1 cut(s)	1946
RsrII	CGGWCCG	1 cut(s)	1946
SacI	GAGCTC	2 cut(s)	49, 69
SaqAI	TTAA	8 cut(s)	269, 545, 630, 1250, 1728, 1760, 1977, 2064
Sau3AI	GATC	7 cut(s)	99, 109, 331, 604, 695, 1280, 1708
SduI	GDGCHC	6 cut(s)	49, 69, 156, 162, 953, 1226
SfaNI	GCATC	6 cut(s)	901, 1141, 1309, 1445, 1857, 2030
SfcI	CTRYAG	5 cut(s)	735, 1053, 1146, 1591, 1998
SfoI	GGCGCC	1 cut(s)	140
Sfr274I	CTCGAG	1 cut(s)	1907
SgrAI	CRCCGGYG	1 cut(s)	1427
SinI	GGWCC	6 cut(s)	169, 1099, 1119, 1292, 1667, 1946
SlaI	CTCGAG	1 cut(s)	1907
SmiMI	CAYNNNNRTG	2 cut(s)	1812, 1925
SmlI	CTYRAG	2 cut(s)	538, 1907
SmoI	CTYRAG	2 cut(s)	538, 1907
SnaBI	TACGTA	1 cut(s)	772
SsiI	CCGC	9 cut(s)	233, 237, 263, 298, 1521, 1786, 1931, 1944, 2137
SspDI	GGCGCC	1 cut(s)	138
SspMI	CTAG	7 cut(s)	342, 404, 680, 960, 1004, 1350, 2073
SstI	GAGCTC	2 cut(s)	49, 69
StyI	CCWWGG	5 cut(s)	82, 1115, 1226, 1263, 2161
TaaI	ACNGT	6 cut(s)	31, 77, 206, 400, 1012, 1698
TaiI	ACGT	4 cut(s)	137, 159, 454, 774
TaqI	TCGA	7 cut(s)	18, 322, 711, 1688, 1819, 1908, 2157
TatI	WGTACW	4 cut(s)	198, 400, 1373, 1691
TauI	GCSGC	2 cut(s)	1933, 2140
TfiI	GAWTC	5 cut(s)	520, 1837, 1878, 2008, 2131
Tru1I	TTAA	8 cut(s)	269, 545, 630, 1250, 1728, 1760, 1977, 2064
Tru9I	TTAA	8 cut(s)	269, 545, 630, 1250, 1728, 1760, 1977, 2064
TscAI	CASTG	3 cut(s)	154, 1618, 1701
TseFI	GTSAC	2 cut(s)	587, 980
TseI	GCWGC	8 cut(s)	119, 1037, 1142, 1145, 1590, 1916, 1954, 2140
Tsp45I	GTSAC	2 cut(s)	587, 980
TspDTI	ATGAA	6 cut(s)	428, 737, 1044, 1464, 1653, 1850
TspGWI	ACGGA	1 cut(s)	1400
TspRI	CASTG	3 cut(s)	154, 1618, 1701
Van91I	CCANNNNNTGG	2 cut(s)	289, 1649
VpaK11BI	GGWCC	6 cut(s)	169, 1099, 1119, 1292, 1667, 1946
XagI	CCTNNNNNAGG	1 cut(s)	1938
XapI	RAATTY	4 cut(s)	828, 1031, 1378, 1981
XbaI	TCTAGA	2 cut(s)	1003, 2072
XceI	RCATGY	2 cut(s)	516, 668
XcmI	CCANNNNNNNNNTGG	2 cut(s)	387, 495
XhoI	CTCGAG	1 cut(s)	1907
XmiI	GTMKAC	1 cut(s)	886
XspI	CTAG	7 cut(s)	342, 404, 680, 960, 1004, 1350, 2073

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.