Rh7BG065300

Acyl-activating enzyme 17

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0010059
Physical Location & Seq
Reverse (-)
4316155 .. 4322188
6034 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG065300.1

Sequence Viewer

Length: 2253 bp
ATGTCGTACAAGAGCCTCGACACCGTTACGGTCGCCGACATCGGAGCTCTCGGGATTGAGCCGGAGCTCGCCAACAGACTCCATGGAAGTCTCGCTAGAATCATCGCCGATCACGGCGCTGCCACTCCGGACACGTGGCGCAGTATCACTGGGCACGTGCTCAGCCCGGACCTCCCCTTCTCCTTCCACCAGATGATGTACTACGGTTGCTATAAGGACTTCGGACCCGACCCGCCCGCCTGGCTCCCCGACCCGGAGACTGCGGTTTTAACAAATGCCGGCCAGCTACTGGAGAGGCAGGGAAAAGAGTTTTTGGGTTCTCGATATAAGGATCCCATATCTAGCTTTTCCGATTTTCAGGAGTTTTCGGTGTCAAACCCAGAGGTTTATTGGAAAACTGTACTAGATGAGATGAATGTGTCGTTTTACAAGCCTCCCCAATGTATTTTACGTGAGAATCTGTCTGGAGATGGGCATTTATCAGTTCCAGGAGTTCAATGGCTTCCTGGAGCATGTGTGAATCCAGCAAAGAATTGCTTGAGTTTAAATTGTAAGAGGAGCTTGAATGATACAATGGTAGTATGGCGTGACGAAGGAAAGGATGATCTGCCTTTGAATAAGATGACACTTAAAGAATTGCGTGCAGAGGTTTGGTTAGTTGCACATGCTCTCCAAGCACTAGGTCTGGAGAAAGGATCTGCAATCGCAATCGATATGCCAATGAATGTCATTTCTGTAGTTATCTACCTGGCTATTGTTCTGGCAGGTTACGTAGTTGTATCAATCGCTGATAGTTTTGCTCCCCCTGAAATATCAACCAGACTAAAAATTTCGGAGGCAAAAGCCATATTTACCCAGGATGTAATAGTTCGTGGTGAGAAAAGTCTACCCTTGTATAGTAAAATCGTGGATGCTCAATCTCCTATGGCCATTGTTCTCCTTACAAGGGGCTCCAACTCTAGTGTGAAATTGCGTGATGGTGACATTTCTTGGCATGATTTTCTAGAAACAGTAAAAGATTTCAAAGATCAATTCGCTGCTGTGGAACAACCAGTAGAAGCATTTACAAATATCCTCTTCTCATCTGGAACTACTGGGGACCCAAAGGCAATTCCATGGACCCATTCAACTCCTTTCAAAGCAGCTGCAGATGCCTGGTGCCACATGGACATTCGTAAAGGTGATGTTATTGCTTGGCCCACAAATCTTGGGTGGATGATGGGCCCTTGGTTGGTATATGCTTCTTTGTTAAATGGTGCTTCCATTGCCCTATACAATGGATCTCCACTTGGACCTGGCTTTTCCAAGTTTGTTCAGGATGCCAAAGTAACAATGCTTGGCGTAATCCCTAGTATTGTTCGGACATGGAAAAGTACAAATTCTACTTCAGGCTATGATTGGTCAGCCATCCGTTGCTTTAGCTCCACCGGTGAAGCATCAAATGTAGACGAATACCTATGGCTGATGGGGAGGGCTGGCTACAAGCCTATCATAGAGTATTGTGGTGGTACAGAAATTGGCGGTGCCTTTGTTTCTGGGTCATTGTTGCAGGCTCAGTCTTTGGCTTCTTTTAGCACTCCAGCTATGGGCTGCAGTTTGTTTATTCTTGGCACTGATGGAAGTCCTATTCCACAAAATGAACCAGGGGTTGGTGAACTGGCGCTTGGTCCCCTCATGTTTGGAGCTTCGAGTACACTGTTGAATGCTGATCATTATGAGGTCTATTTTAAGGGAATGCCATTATGGAATGGGAAGGTAAACAACTTTCTTCTCCACTTTCAAACCAGTCGACCTTCTAAAACACTTATAATTTCTGTTTTTCACTTGCAGGTTTTAAGAAGACATGGGGATTTGTTTGAGCGGACATCCAGGGGTTACTATCATGCTCATGGTCGAGCAGATGATACGATGAATCTTGGAGGCATCAAGGTTAGTTCTGTTGAGATTGAAAGAATCTGTAATGCAATTGACACCAACATCCTCGAGACAGCTGCCATTGGGGTGCCGCCTGCTGGAGGCGGACCGGAGCAGCTGGTAATTGCTGTTGTATCTAAGAATTCAGATAATCCACCTGCAGAATTGAATCAAGTAAGAGCATCCTTCAATTCAGCTGTGCAGAAGAAACTAAATCCCTTGTTTAAGGTTTCTAGAGTTGTGCCCCTCCCTTCTCTTCCAAGAACAGCAACAAATAAGGTCATGAGAAGGATTCTGCGGCAGCAGTTTGCTCAACTCGACCAAGGTGCTAAGCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ACAS_N AMP-binding AMP-binding_C

Protein Analysis

750

Amino Acids

81.89

Weight (kDa)

6.23

Isoelectric Point (pI)

33.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 205 - 554 1.2e-44 AMP-binding enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1808
AarI CACCTGC 1 cut(s) 2080
Acc36I ACCTGC 3 cut(s) 755, 1819, 2080
AccB1I GGYRCC 3 cut(s) 1158, 1523, 2002
AccB7I CCANNNNNTGG 2 cut(s) 289, 1649
AccBSI CCGCTC 1 cut(s) 1861
AccI GTMKAC 3 cut(s) 886, 1446, 1789
AccIII TCCGGA 1 cut(s) 127
AciI CCGC 8 cut(s) 233, 237, 263, 1521, 1861, 2006, 2019, 2212
AclWI GGATC 4 cut(s) 326, 339, 703, 1288
AcoI YGGCCR 2 cut(s) 280, 927
AcsI RAATTY 3 cut(s) 828, 1378, 2056
AcuI CTGAAG 1 cut(s) 1371
AcvI CACGTG 2 cut(s) 135, 157
AfaI GTAC 6 cut(s) 8, 200, 402, 1375, 1510, 1693
AfiI CCNNNNNNNGG 8 cut(s) 253, 289, 946, 1231, 1586, 1649, 2012, 2015
AflIII ACRYGT 1 cut(s) 132
AgeI ACCGGT 1 cut(s) 1427
AjnI CCWGG 9 cut(s) 239, 487, 505, 747, 855, 1154, 1294, 1642, 1868
AjuI GAANNNNNNNTTGG 4 cut(s) 1632, 1647, 1664, 1679
Alw21I GWGCWC 3 cut(s) 49, 69, 162
Alw26I GTCTC 3 cut(s) 95, 251, 1979
AlwI GGATC 4 cut(s) 326, 339, 703, 1288
AlwNI CAGNNNCTG 1 cut(s) 289
Ama87I CYCGRG 2 cut(s) 50, 1982
Aor13HI TCCGGA 1 cut(s) 127
AoxI GGCC 4 cut(s) 280, 927, 1196, 1222
ApaI GGGCCC 1 cut(s) 1226
ApeKI GCWGC 8 cut(s) 119, 1037, 1142, 1145, 1590, 1991, 2029, 2215
ApoI RAATTY 3 cut(s) 828, 1378, 2056
AsiGI ACCGGT 1 cut(s) 1427
AspLEI GCGC 3 cut(s) 119, 141, 1663
AsuC2I CCSGG 2 cut(s) 167, 254
AsuHPI GGTGA 5 cut(s) 887, 992, 1193, 1442, 1664
AvaI CYCGRG 2 cut(s) 50, 1982
AvaII GGWCC 7 cut(s) 169, 224, 1099, 1119, 1292, 1667, 2021
BaeGI GKGCMC 3 cut(s) 156, 1226, 2160
BaeI ACNNNNGTAYC 2 cut(s) 561, 594
BalI TGGCCA 1 cut(s) 929
BamHI GGATCC 1 cut(s) 331
BanI GGYRCC 3 cut(s) 1158, 1523, 2002
BanII GRGCYC 4 cut(s) 49, 69, 953, 1226
BbrPI CACGTG 2 cut(s) 135, 157
BbsI GAAGAC 1 cut(s) 1846
Bbv12I GWGCWC 3 cut(s) 49, 69, 162
BbvI GCAGC 8 cut(s) 106, 1024, 1132, 1154, 1577, 1978, 2041, 2227
BccI CCATC 6 cut(s) 464, 971, 1213, 1415, 1459, 1610
BceAI ACGGC 1 cut(s) 130
BcgI CGANNNNNNTGC 3 cut(s) 1973, 2007, 2222
BciT130I CCWGG 9 cut(s) 241, 489, 507, 749, 857, 1156, 1296, 1644, 1870
BclI TGATCA 1 cut(s) 1708
BcnI CCSGG 2 cut(s) 167, 254
BcoDI GTCTC 3 cut(s) 95, 251, 1979
BfaI CTAG 8 cut(s) 96, 342, 404, 680, 960, 1004, 1350, 2148
BfmI CTRYAG 4 cut(s) 735, 1146, 1591, 2073
BfoI RGCGCY 2 cut(s) 120, 1664
BfuAI ACCTGC 3 cut(s) 755, 1819, 2080
BglI GCCNNNNNGGC 1 cut(s) 241
BlpI GCTNAGC 2 cut(s) 161, 2244
Bme18I GGWCC 7 cut(s) 169, 224, 1099, 1119, 1292, 1667, 2021
BmeT110I CYCGRG 2 cut(s) 50, 1982
BmrI ACTGGG 2 cut(s) 159, 1104
BmsI GCATC 6 cut(s) 901, 1141, 1309, 1445, 1932, 2105
BmuI ACTGGG 2 cut(s) 159, 1104
BpiI GAAGAC 1 cut(s) 1846
BpmI CTGGAG 6 cut(s) 311, 486, 528, 707, 1563, 2034
Bpu1102I GCTNAGC 2 cut(s) 161, 2244
BpuEI CTTGAG 1 cut(s) 559
BpuMI CCSGG 2 cut(s) 167, 254
Bsa29I ATCGAT 1 cut(s) 711
BsaAI YACGTR 4 cut(s) 135, 157, 452, 772
BsaJI CCNNGG 7 cut(s) 82, 855, 1115, 1226, 1643, 1869, 2236
BsaWI WCCGGW 3 cut(s) 127, 1427, 2023
BsaXI ACNNNNNCTCC 4 cut(s) 501, 531, 654, 684
Bsc4I CCNNNNNNNGG 8 cut(s) 253, 289, 946, 1231, 1586, 1649, 2012, 2015
Bse118I RCCGGY 2 cut(s) 278, 1427
Bse1I ACTGG 6 cut(s) 154, 294, 1052, 1099, 1662, 1785
Bse3DI GCAATG 1 cut(s) 1263
BseAI TCCGGA 1 cut(s) 127
BseBI CCWGG 9 cut(s) 241, 489, 507, 749, 857, 1156, 1296, 1644, 1870
BseCI ATCGAT 1 cut(s) 711
BseDI CCNNGG 7 cut(s) 82, 855, 1115, 1226, 1643, 1869, 2236
BseGI GGATG 9 cut(s) 607, 865, 916, 1221, 1324, 1407, 1865, 1977, 2096
BseLI CCNNNNNNNGG 8 cut(s) 253, 289, 946, 1231, 1586, 1649, 2012, 2015
BseMI GCAATG 1 cut(s) 1263
BseMII CTCAG 2 cut(s) 175, 1568
BseNI ACTGG 6 cut(s) 154, 294, 1052, 1099, 1662, 1785
BseRI GAGGAG 1 cut(s) 571
BseSI GKGCMC 3 cut(s) 156, 1226, 2160
BseXI GCAGC 8 cut(s) 106, 1024, 1132, 1154, 1577, 1978, 2041, 2227
BsgI GTGCAG 2 cut(s) 663, 2135
Bsh1285I CGRYCG 1 cut(s) 33
BshFI GGCC 4 cut(s) 282, 929, 1198, 1224
BshNI GGYRCC 3 cut(s) 1158, 1523, 2002
BshTI ACCGGT 1 cut(s) 1427
BshVI ATCGAT 1 cut(s) 711
BsiEI CGRYCG 1 cut(s) 33
BsiHKAI GWGCWC 3 cut(s) 49, 69, 162
BsiHKCI CYCGRG 2 cut(s) 50, 1982
BsiSI CCGG 7 cut(s) 62, 128, 167, 254, 279, 1428, 2024
BslFI GGGAC 2 cut(s) 1112, 1653
BslI CCNNNNNNNGG 8 cut(s) 253, 289, 946, 1231, 1586, 1649, 2012, 2015
BsmAI GTCTC 3 cut(s) 95, 251, 1979
BsmFI GGGAC 2 cut(s) 1112, 1653
BsmI GAATGC 2 cut(s) 1708, 1740
BsnI GGCC 4 cut(s) 282, 929, 1198, 1224
BsoBI CYCGRG 2 cut(s) 50, 1982
Bsp120I GGGCCC 1 cut(s) 1222
Bsp1286I GDGCHC 7 cut(s) 49, 69, 156, 162, 953, 1226, 2160
Bsp13I TCCGGA 1 cut(s) 127
Bsp143I GATC 7 cut(s) 109, 331, 604, 695, 1027, 1280, 1708
Bsp1720I GCTNAGC 2 cut(s) 161, 2244
Bsp19I CCATGG 2 cut(s) 82, 1115
BspACI CCGC 8 cut(s) 233, 237, 263, 1521, 1861, 2006, 2019, 2212
BspANI GGCC 4 cut(s) 282, 929, 1198, 1224
BspCNI CTCAG 2 cut(s) 174, 1567
BspDI ATCGAT 1 cut(s) 711
BspEI TCCGGA 1 cut(s) 127
BspHI TCATGA 1 cut(s) 2196
BspMAI CTGCAG 3 cut(s) 1150, 1595, 2077
BspMI ACCTGC 3 cut(s) 755, 1819, 2080
BspPI GGATC 4 cut(s) 326, 339, 703, 1288
BspT107I GGYRCC 3 cut(s) 1158, 1523, 2002
BsrBI CCGCTC 1 cut(s) 1861
BsrDI GCAATG 1 cut(s) 1263
BsrFI RCCGGY 2 cut(s) 278, 1427
BsrI ACTGG 6 cut(s) 154, 294, 1052, 1099, 1662, 1785
BssAI RCCGGY 2 cut(s) 278, 1427
BssECI CCNNGG 7 cut(s) 82, 855, 1115, 1226, 1643, 1869, 2236
BssMI GATC 7 cut(s) 109, 331, 604, 695, 1027, 1280, 1708
BssT1I CCWWGG 4 cut(s) 82, 1115, 1226, 2236
Bst2UI CCWGG 9 cut(s) 241, 489, 507, 749, 857, 1156, 1296, 1644, 1870
Bst4CI ACNGT 6 cut(s) 25, 31, 206, 400, 1012, 1698
Bst6I CTCTTC 2 cut(s) 1082, 2175
BstBAI YACGTR 4 cut(s) 135, 157, 452, 772
BstC8I GCNNGC 8 cut(s) 69, 237, 280, 284, 642, 1477, 1551, 2010
BstDEI CTNAG 4 cut(s) 161, 1554, 2052, 2244
BstDSI CCRYGG 2 cut(s) 82, 1115
BstENI CCTNNNNNAGG 1 cut(s) 2013
BstF5I GGATG 9 cut(s) 607, 865, 916, 1221, 1324, 1407, 1865, 1977, 2096
BstH2I RGCGCY 2 cut(s) 120, 1664
BstHHI GCGC 3 cut(s) 119, 141, 1663
BstKTI GATC 7 cut(s) 112, 334, 607, 698, 1030, 1283, 1711
BstMAI GTCTC 3 cut(s) 95, 251, 1979
BstMBI GATC 7 cut(s) 109, 331, 604, 695, 1027, 1280, 1708
BstMCI CGRYCG 1 cut(s) 33
BstMWI GCNNNNNNNGC 4 cut(s) 241, 674, 1151, 1265
BstNI CCWGG 9 cut(s) 241, 489, 507, 749, 857, 1156, 1296, 1644, 1870
BstNSI RCATGY 2 cut(s) 516, 668
BstSFI CTRYAG 4 cut(s) 735, 1146, 1591, 2073
BstSLI GKGCMC 3 cut(s) 156, 1226, 2160
BstSNI TACGTA 1 cut(s) 772
BstV1I GCAGC 8 cut(s) 106, 1024, 1132, 1154, 1577, 1978, 2041, 2227
BstV2I GAAGAC 1 cut(s) 1846
BstX2I RGATCY 3 cut(s) 331, 695, 1280
BstYI RGATCY 3 cut(s) 331, 695, 1280
Bsu15I ATCGAT 1 cut(s) 711
BsuRI GGCC 4 cut(s) 282, 929, 1198, 1224
BsuTUI ATCGAT 1 cut(s) 711
BtgI CCRYGG 2 cut(s) 82, 1115
BtgZI GCGATG 1 cut(s) 88
BtsCI GGATG 9 cut(s) 607, 865, 916, 1221, 1324, 1407, 1865, 1977, 2096
BtsIMutI CAGTG 3 cut(s) 147, 1611, 1694
BveI ACCTGC 3 cut(s) 755, 1819, 2080
Cac8I GCNNGC 8 cut(s) 69, 237, 280, 284, 642, 1477, 1551, 2010
CaiI CAGNNNCTG 1 cut(s) 289
CciI TCATGA 1 cut(s) 2196
CfoI GCGC 3 cut(s) 119, 141, 1663
Cfr10I RCCGGY 2 cut(s) 278, 1427
ClaI ATCGAT 1 cut(s) 711
CpoI CGGWCCG 1 cut(s) 2021
Csp6I GTAC 6 cut(s) 7, 199, 401, 1374, 1509, 1692
CspAI ACCGGT 1 cut(s) 1427
CspCI CAANNNNNGTGG 2 cut(s) 1189, 1224
CspI CGGWCCG 1 cut(s) 2021
CviQI GTAC 6 cut(s) 7, 199, 401, 1374, 1509, 1692
DdeI CTNAG 4 cut(s) 161, 1554, 2052, 2244
DpnI GATC 7 cut(s) 111, 333, 606, 697, 1029, 1282, 1710
DpnII GATC 7 cut(s) 109, 331, 604, 695, 1027, 1280, 1708
DraI TTTAAA 1 cut(s) 546
EaeI YGGCCR 2 cut(s) 280, 927
Eam1104I CTCTTC 2 cut(s) 1082, 2175
EarI CTCTTC 2 cut(s) 1082, 2175
EciI GGCGGA 1 cut(s) 2034
Ecl136II GAGCTC 2 cut(s) 47, 67
Eco105I TACGTA 1 cut(s) 772
Eco130I CCWWGG 4 cut(s) 82, 1115, 1226, 2236
Eco24I GRGCYC 4 cut(s) 49, 69, 953, 1226
Eco47I GGWCC 7 cut(s) 169, 224, 1099, 1119, 1292, 1667, 2021
Eco53kI GAGCTC 2 cut(s) 47, 67
Eco57I CTGAAG 1 cut(s) 1371
Eco72I CACGTG 2 cut(s) 135, 157
Eco88I CYCGRG 2 cut(s) 50, 1982
EcoICRI GAGCTC 2 cut(s) 47, 67
EcoNI CCTNNNNNAGG 1 cut(s) 2013
EcoO109I RGGNCCY 2 cut(s) 1099, 1223
EcoRI GAATTC 1 cut(s) 2056
EcoRII CCWGG 9 cut(s) 239, 487, 505, 747, 855, 1154, 1294, 1642, 1868
EcoT14I CCWWGG 4 cut(s) 82, 1115, 1226, 2236
EcoT38I GRGCYC 4 cut(s) 49, 69, 953, 1226
ErhI CCWWGG 4 cut(s) 82, 1115, 1226, 2236
FalI AAGNNNNNCTT 4 cut(s) 521, 553, 545, 577
FaqI GGGAC 2 cut(s) 1112, 1653
FauI CCCGC 2 cut(s) 240, 244
FbaI TGATCA 1 cut(s) 1708
FblI GTMKAC 3 cut(s) 886, 1446, 1789
FokI GGATG 9 cut(s) 614, 872, 923, 1228, 1331, 1394, 1852, 1964, 2083
FriOI GRGCYC 4 cut(s) 49, 69, 953, 1226
FspBI CTAG 8 cut(s) 96, 342, 404, 680, 960, 1004, 1350, 2148
GlaI GCGC 3 cut(s) 118, 140, 1662
GsuI CTGGAG 6 cut(s) 311, 486, 528, 707, 1563, 2034
HaeII RGCGCY 2 cut(s) 120, 1664
HaeIII GGCC 4 cut(s) 282, 929, 1198, 1224
HapII CCGG 7 cut(s) 62, 128, 167, 254, 279, 1428, 2024
HhaI GCGC 3 cut(s) 119, 141, 1663
Hin6I GCGC 3 cut(s) 117, 139, 1661
HinP1I GCGC 3 cut(s) 117, 139, 1661
HincII GTYRAC 1 cut(s) 1790
HindII GTYRAC 1 cut(s) 1790
HinfI GANTC 8 cut(s) 78, 99, 457, 520, 1912, 1953, 2083, 2206
HpaII CCGG 7 cut(s) 62, 128, 167, 254, 279, 1428, 2024
HphI GGTGA 5 cut(s) 887, 992, 1193, 1442, 1664
Hpy166II GTNNAC 6 cut(s) 887, 1447, 1655, 1694, 1759, 1790
Hpy188I TCNGA 6 cut(s) 44, 224, 352, 835, 1362, 2062
Hpy8I GTNNAC 6 cut(s) 887, 1447, 1655, 1694, 1759, 1790
HpyAV CCTTC 8 cut(s) 187, 193, 587, 1747, 1803, 2110, 2175, 2196
HpyCH4III ACNGT 6 cut(s) 25, 31, 206, 400, 1012, 1698
HpyCH4IV ACGT 4 cut(s) 134, 156, 451, 771
HpyF10VI GCNNNNNNNGC 4 cut(s) 241, 674, 1151, 1265
HpyF3I CTNAG 4 cut(s) 161, 1554, 2052, 2244
HpySE526I ACGT 4 cut(s) 134, 156, 451, 771
HspAI GCGC 3 cut(s) 117, 139, 1661
KflI GGGWCCC 1 cut(s) 1099
Kpn2I TCCGGA 1 cut(s) 127
KroI GCCGGC 1 cut(s) 278
KroNI GCCGGC 1 cut(s) 280
Ksp22I TGATCA 1 cut(s) 1708
Kzo9I GATC 7 cut(s) 109, 331, 604, 695, 1027, 1280, 1708
Lsp1109I GCAGC 8 cut(s) 106, 1024, 1132, 1154, 1577, 1978, 2041, 2227
LweI GCATC 6 cut(s) 901, 1141, 1309, 1445, 1932, 2105
MaeI CTAG 8 cut(s) 96, 342, 404, 680, 960, 1004, 1350, 2148
MaeII ACGT 4 cut(s) 134, 156, 451, 771
MaeIII GTNAC 6 cut(s) 25, 587, 767, 980, 1327, 1874
MalI GATC 7 cut(s) 111, 333, 606, 697, 1029, 1282, 1710
MbiI CCGCTC 1 cut(s) 1861
MboI GATC 7 cut(s) 109, 331, 604, 695, 1027, 1280, 1708
MboII GAAGA 5 cut(s) 1069, 1760, 1851, 2131, 2162
MfeI CAATTG 1 cut(s) 1965
MflI RGATCY 3 cut(s) 331, 695, 1280
MhlI GDGCHC 7 cut(s) 49, 69, 156, 162, 953, 1226, 2160
MlsI TGGCCA 1 cut(s) 929
MluNI TGGCCA 1 cut(s) 929
MlyI GAGTC 1 cut(s) 72
MmeI TCCRAC 1 cut(s) 978
Mox20I TGGCCA 1 cut(s) 929
MroI TCCGGA 1 cut(s) 127
MroNI GCCGGC 1 cut(s) 278
MscI TGGCCA 1 cut(s) 929
MseI TTAA 7 cut(s) 269, 545, 630, 1250, 1728, 1835, 2139
MslI CAYNNNNRTG 2 cut(s) 1887, 2000
Msp20I TGGCCA 1 cut(s) 929
MspA1I CMGCKG 4 cut(s) 1145, 1991, 2032, 2111
MspI CCGG 7 cut(s) 62, 128, 167, 254, 279, 1428, 2024
MunI CAATTG 1 cut(s) 1965
Mva1269I GAATGC 2 cut(s) 1708, 1740
MvaI CCWGG 9 cut(s) 241, 489, 507, 749, 857, 1156, 1296, 1644, 1870
MwoI GCNNNNNNNGC 4 cut(s) 241, 674, 1151, 1265
NaeI GCCGGC 1 cut(s) 280
NciI CCSGG 2 cut(s) 167, 254
NcoI CCATGG 2 cut(s) 82, 1115
NdeII GATC 7 cut(s) 109, 331, 604, 695, 1027, 1280, 1708
NgoMIV GCCGGC 1 cut(s) 278
NmuCI GTSAC 2 cut(s) 587, 980
NspI RCATGY 2 cut(s) 516, 668
PaeR7I CTCGAG 1 cut(s) 1982
PagI TCATGA 1 cut(s) 2196
PaqCI CACCTGC 1 cut(s) 2080
PctI GAATGC 2 cut(s) 1708, 1740
PdiI GCCGGC 1 cut(s) 280
PfeI GAWTC 7 cut(s) 99, 457, 520, 1912, 1953, 2083, 2206
PflMI CCANNNNNTGG 2 cut(s) 289, 1649
PfoI TCCNGGA 2 cut(s) 487, 505
PinAI ACCGGT 1 cut(s) 1427
PleI GAGTC 1 cut(s) 72
PmaCI CACGTG 2 cut(s) 135, 157
PmlI CACGTG 2 cut(s) 135, 157
PpsI GAGTC 1 cut(s) 72
Ppu21I YACGTR 4 cut(s) 135, 157, 452, 772
PpuMI RGGWCCY 1 cut(s) 1099
PsiI TTATAA 1 cut(s) 1808
Psp124BI GAGCTC 2 cut(s) 49, 69
Psp5II RGGWCCY 1 cut(s) 1099
Psp6I CCWGG 9 cut(s) 239, 487, 505, 747, 855, 1154, 1294, 1642, 1868
PspCI CACGTG 2 cut(s) 135, 157
PspGI CCWGG 9 cut(s) 239, 487, 505, 747, 855, 1154, 1294, 1642, 1868
PspOMI GGGCCC 1 cut(s) 1222
PspPPI RGGWCCY 1 cut(s) 1099
PstI CTGCAG 3 cut(s) 1150, 1595, 2077
PstNI CAGNNNCTG 1 cut(s) 289
PsuI RGATCY 3 cut(s) 331, 695, 1280
PvuII CAGCTG 4 cut(s) 1145, 1991, 2032, 2111
RsaI GTAC 6 cut(s) 8, 200, 402, 1375, 1510, 1693
RsaNI GTAC 6 cut(s) 7, 199, 401, 1374, 1509, 1692
RseI CAYNNNNRTG 2 cut(s) 1887, 2000
Rsr2I CGGWCCG 1 cut(s) 2021
RsrII CGGWCCG 1 cut(s) 2021
SacI GAGCTC 2 cut(s) 49, 69
SalI GTCGAC 1 cut(s) 1788
SaqAI TTAA 7 cut(s) 269, 545, 630, 1250, 1728, 1835, 2139
Sau3AI GATC 7 cut(s) 109, 331, 604, 695, 1027, 1280, 1708
SchI GAGTC 1 cut(s) 72
SduI GDGCHC 7 cut(s) 49, 69, 156, 162, 953, 1226, 2160
SfaNI GCATC 6 cut(s) 901, 1141, 1309, 1445, 1932, 2105
SfcI CTRYAG 4 cut(s) 735, 1146, 1591, 2073
Sfr274I CTCGAG 1 cut(s) 1982
SgrAI CRCCGGYG 1 cut(s) 1427
SinI GGWCC 7 cut(s) 169, 224, 1099, 1119, 1292, 1667, 2021
SlaI CTCGAG 1 cut(s) 1982
SmiMI CAYNNNNRTG 2 cut(s) 1887, 2000
SmlI CTYRAG 2 cut(s) 538, 1982
SmoI CTYRAG 2 cut(s) 538, 1982
SnaBI TACGTA 1 cut(s) 772
SsiI CCGC 8 cut(s) 233, 237, 263, 1521, 1861, 2006, 2019, 2212
SspMI CTAG 8 cut(s) 96, 342, 404, 680, 960, 1004, 1350, 2148
SstI GAGCTC 2 cut(s) 49, 69
StyI CCWWGG 4 cut(s) 82, 1115, 1226, 2236
TaaI ACNGT 6 cut(s) 25, 31, 206, 400, 1012, 1698
TaiI ACGT 4 cut(s) 137, 159, 454, 774
TaqI TCGA 8 cut(s) 18, 322, 711, 1688, 1789, 1894, 1983, 2232
TatI WGTACW 4 cut(s) 198, 400, 1373, 1691
TauI GCSGC 2 cut(s) 2008, 2215
TfiI GAWTC 7 cut(s) 99, 457, 520, 1912, 1953, 2083, 2206
Tru1I TTAA 7 cut(s) 269, 545, 630, 1250, 1728, 1835, 2139
Tru9I TTAA 7 cut(s) 269, 545, 630, 1250, 1728, 1835, 2139
TscAI CASTG 3 cut(s) 154, 1618, 1701
TseFI GTSAC 2 cut(s) 587, 980
TseI GCWGC 8 cut(s) 119, 1037, 1142, 1145, 1590, 1991, 2029, 2215
Tsp45I GTSAC 2 cut(s) 587, 980
TspDTI ATGAA 4 cut(s) 428, 737, 1653, 1925
TspGWI ACGGA 1 cut(s) 1400
TspRI CASTG 3 cut(s) 154, 1618, 1701
Van91I CCANNNNNTGG 2 cut(s) 289, 1649
VpaK11BI GGWCC 7 cut(s) 169, 224, 1099, 1119, 1292, 1667, 2021
XagI CCTNNNNNAGG 1 cut(s) 2013
XapI RAATTY 3 cut(s) 828, 1378, 2056
XbaI TCTAGA 2 cut(s) 1003, 2147
XceI RCATGY 2 cut(s) 516, 668
XcmI CCANNNNNNNNNTGG 2 cut(s) 387, 495
XhoI CTCGAG 1 cut(s) 1982
XmiI GTMKAC 3 cut(s) 886, 1446, 1789
XspI CTAG 8 cut(s) 96, 342, 404, 680, 960, 1004, 1350, 2148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.