RchiOBHm_Chr7g0184211

Acyl-activating enzyme 17

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0010059
Physical Location & Seq
Reverse (-)
4884730 .. 4890652
5923 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ16437

Sequence Viewer

Length: 2178 bp
ATGTCGTACAAGAGCCTCGACGCCGTTACGGTCGCCGACATCGCAGCTCTCGGGATTGAGCCGGAGCTCGCCAACAGACTCCATGGAAGTCTCGCTAAGATCATCGCCGATCACGGCGCTGCCACTCCGGACACGTGGCGCAGTATCACTGGGCACGTGCTCAGCCCGGACCTCCCCTTCTCCTTCCACCAGATGATGTACTACGGTTGCTATAAGGACTTCGGACCCGACCCGCCCGCCTGGCTCCCCGACCCGGAGACTGCGGTTTTAACAAATGCCGGCCAGCTACTGGAGAGGCGGGGAAAAGAGTTTTTGGGTTCTCAATATAAGGATCCCATATCTAGCTTTTCCGATTTTCAGGAGTTTTCGGTGTCAAACCCAGAGGTTTATTGGAAAACTGTACTAGATGAGATGAATGTGTCGTTTTACAAGCCGCCCCAATGTATTTTACGTGAGAATCTGTCTGGAGATGGGCATTTATTAGTTCCAGGAGTTCAATGGCTTCCTGGAGCATGTGTGAATCCAGCAAAGAATTGCTTGAGTTTAAATTCTAAGAGGAGCTTGAATGATACAATGGTAGTATGGCGTGACGAAGGAAAGGATGATCTGCCTTTGAATAAGATGACACTTAAAGAATTGCGTGCAGAGGTTTGGTTAGTTGCACATGCTCTCCAAGCACAAGGTCTGGAGAAAGGATCTGCAATCGCAATCGATATGCCAATGAATGTCATTTCTGTAGTTATCTACCTGGCTATTGTTCTGGCAGGTTACGTAGTTGTATCAATCGCTGATAGTTTTGCTCCCCCTGAAATATCAACCAGACTAAAAATTTCGGAGGCAAAAGCCATATTTACCCAGGATGTAATAGTTCGTGGTGAGAAAAGTCTACCCTTGTATAGTAAAATCGTGGATGCTCAATCTCCTATGGCCATTGTTCTCCTTACAAGGGGCTCCAAATCTAGTGTGAAATTGCGTGATGGTGACATTTCTTGGCATGATTTTCTAAACACAGTAAAAGATTTCAAAGATGAATTCGCTGCTGTGGAACAACCTGTAGAAGCATTTACAAATATCCTCTTCTCATCTGGAACTACTGGGGACCCAAAGGCAATTCCATGGACCCATTCAACTCCTTTCAAAGCAGCTGCAGATGCCTGGTGCCACATGGACATTCGTAAAGGTGATGTTATTGCTTGGCCCACAAATCTTGGGTGGATGATGGGTCCTTGGTTGGTGTATGCTTCGTTGTTAAATGTTGCTTCCATTGCCCTATACAATGGATCTCCACTTGGACCTGGCTTTTCCAAGTTTGTTCAGGATGCCAAAGTAACAATGCTTGGCGTAATCCCTAGTATTGTTCGGACATGGAAAAGTACAAATTCTACTTCAGGCTATGATTGGTCAGCCATCCGTTGCTTTAGCTCCACCGGTGAAGCATCAAATGTAGACGAATACCTATGGCTGATGGGGAGGGCTGGATACAAGCCTATCATAGAGTATTGTGGTGGTACAGAAATTGGCGGTGCCTTTGTTTCTGGGTCATTGTTGCAGGCTCAGTCTTTGGCTTCTTTTAGCACTCCAGCTATGGGCTGCAGTTTGTTTATTCTTGGCACTGATGGAAGTCCTATTCCACAAAATGAACCAGGGGTTGGTGAACTGGCGCTTGGTCCCCTCATGTTTGGAGCTTCGAGTACACTGTTGAATGCTGATCATTATGAGGTCTATTTTAAGGGAATGCCATTATGGAATGGGAAGGTTTTGAGAAGACATGGGGATTTGTTTGAGCGGACATCCAGGGGTTACTATCATGCTCATGGTCGAGCAGATGATACAATGAATCTTGGAGGCATCAAGGTTAGTTCTGTTGAGATTGAACGAATCTGTAATGCAATTGACACCAACATCCTCGAGACAGCTGCCATTGGGGTGCCGCCTGCTGGAGGCGGACCAGAGCAGCTGGTAATTGCTGTTGTATTTAAGAATTCAGATAATCCACCTGCAGATTTGAATCAATTAAGAGCATCCTTCAATTCAGCTGTGCAGAAGAAACTAAATCCCTTGTTTAAGGTTTCTAGAGTTGTGCCCCTCCCTTCTCTTCCAAGAACAGCAACAAATAAGGTCATGAGAAGGATTCTGCGGCAGCAGTTTGCTCAAGTAGACCAAGGTGCTAAGCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ACAS_N AMP-binding AMP-binding_C

Protein Analysis

725

Amino Acids

79.04

Weight (kDa)

6.05

Isoelectric Point (pI)

34.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 206 - 554 2.7e-42 AMP-binding enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2005
Acc36I ACCTGC 2 cut(s) 755, 2005
AccB1I GGYRCC 3 cut(s) 1158, 1523, 1927
AccB7I CCANNNNNTGG 2 cut(s) 289, 1649
AccBSI CCGCTC 1 cut(s) 1786
AccI GTMKAC 3 cut(s) 886, 1446, 2157
AccIII TCCGGA 1 cut(s) 127
AclWI GGATC 4 cut(s) 326, 339, 703, 1288
AcoI YGGCCR 2 cut(s) 280, 927
AcsI RAATTY 5 cut(s) 547, 828, 1031, 1378, 1981
AcuI CTGAAG 1 cut(s) 1371
AcvI CACGTG 2 cut(s) 135, 157
AcyI GRCGYC 1 cut(s) 21
AfaI GTAC 6 cut(s) 8, 200, 402, 1375, 1510, 1693
AfiI CCNNNNNNNGG 7 cut(s) 253, 289, 946, 1586, 1649, 1937, 1940
AflIII ACRYGT 1 cut(s) 132
AgeI ACCGGT 1 cut(s) 1427
AjnI CCWGG 9 cut(s) 239, 487, 505, 747, 855, 1154, 1294, 1642, 1793
AjuI GAANNNNNNNTTGG 4 cut(s) 1632, 1647, 1664, 1679
Alw21I GWGCWC 2 cut(s) 69, 162
Alw26I GTCTC 3 cut(s) 95, 251, 1904
AlwI GGATC 4 cut(s) 326, 339, 703, 1288
AlwNI CAGNNNCTG 1 cut(s) 289
Ama87I CYCGRG 2 cut(s) 50, 1907
Aor13HI TCCGGA 1 cut(s) 127
AoxI GGCC 3 cut(s) 280, 927, 1196
ApeKI GCWGC 9 cut(s) 44, 119, 1037, 1142, 1145, 1590, 1916, 1954, 2140
ApoI RAATTY 5 cut(s) 547, 828, 1031, 1378, 1981
AsiGI ACCGGT 1 cut(s) 1427
AspLEI GCGC 3 cut(s) 119, 141, 1663
AspS9I GGNCC 9 cut(s) 169, 224, 1099, 1119, 1197, 1223, 1292, 1667, 1946
AsuC2I CCSGG 2 cut(s) 167, 254
AsuHPI GGTGA 5 cut(s) 887, 992, 1193, 1442, 1664
AvaI CYCGRG 2 cut(s) 50, 1907
AvaII GGWCC 8 cut(s) 169, 224, 1099, 1119, 1223, 1292, 1667, 1946
BaeGI GKGCMC 2 cut(s) 156, 2085
BaeI ACNNNNGTAYC 2 cut(s) 561, 594
BalI TGGCCA 1 cut(s) 929
BamHI GGATCC 1 cut(s) 331
BanI GGYRCC 3 cut(s) 1158, 1523, 1927
BanII GRGCYC 2 cut(s) 69, 953
BbrPI CACGTG 2 cut(s) 135, 157
BbsI GAAGAC 1 cut(s) 1771
Bbv12I GWGCWC 2 cut(s) 69, 162
BbvI GCAGC 9 cut(s) 56, 106, 1024, 1132, 1154, 1577, 1903, 1966, 2152
BccI CCATC 6 cut(s) 464, 971, 1213, 1415, 1459, 1610
BceAI ACGGC 2 cut(s) 8, 130
BcgI CGANNNNNNTGC 2 cut(s) 1898, 1932
BciT130I CCWGG 9 cut(s) 241, 489, 507, 749, 857, 1156, 1296, 1644, 1795
BciVI GTATCC 1 cut(s) 1472
BclI TGATCA 1 cut(s) 1708
BcnI CCSGG 2 cut(s) 167, 254
BcoDI GTCTC 3 cut(s) 95, 251, 1904
BfaI CTAG 5 cut(s) 342, 404, 960, 1350, 2073
BfmI CTRYAG 5 cut(s) 735, 1053, 1146, 1591, 1998
BfoI RGCGCY 2 cut(s) 120, 1664
BfuAI ACCTGC 2 cut(s) 755, 2005
BfuI GTATCC 1 cut(s) 1472
BglI GCCNNNNNGGC 1 cut(s) 241
BlpI GCTNAGC 2 cut(s) 161, 2169
Bme18I GGWCC 8 cut(s) 169, 224, 1099, 1119, 1223, 1292, 1667, 1946
BmeT110I CYCGRG 2 cut(s) 50, 1907
BmgT120I GGNCC 9 cut(s) 169, 224, 1099, 1119, 1197, 1223, 1292, 1667, 1946
BmrI ACTGGG 2 cut(s) 159, 1104
BmsI GCATC 6 cut(s) 901, 1141, 1309, 1445, 1857, 2030
BmuI ACTGGG 2 cut(s) 159, 1104
BpiI GAAGAC 1 cut(s) 1771
BpmI CTGGAG 6 cut(s) 311, 486, 528, 707, 1563, 1959
Bpu1102I GCTNAGC 2 cut(s) 161, 2169
BpuEI CTTGAG 2 cut(s) 559, 2136
BpuMI CCSGG 2 cut(s) 167, 254
Bsa29I ATCGAT 1 cut(s) 711
BsaAI YACGTR 4 cut(s) 135, 157, 452, 772
BsaBI GATNNNNATC 1 cut(s) 2007
BsaHI GRCGYC 1 cut(s) 21
BsaJI CCNNGG 7 cut(s) 82, 855, 1115, 1226, 1643, 1794, 2161
BsaWI WCCGGW 2 cut(s) 127, 1427
BsaXI ACNNNNNCTCC 4 cut(s) 501, 531, 654, 684
Bsc4I CCNNNNNNNGG 7 cut(s) 253, 289, 946, 1586, 1649, 1937, 1940
Bse118I RCCGGY 2 cut(s) 278, 1427
Bse1I ACTGG 4 cut(s) 154, 294, 1099, 1662
Bse3DI GCAATG 1 cut(s) 1263
Bse8I GATNNNNATC 1 cut(s) 2007
BseAI TCCGGA 1 cut(s) 127
BseBI CCWGG 9 cut(s) 241, 489, 507, 749, 857, 1156, 1296, 1644, 1795
BseCI ATCGAT 1 cut(s) 711
BseDI CCNNGG 7 cut(s) 82, 855, 1115, 1226, 1643, 1794, 2161
BseGI GGATG 9 cut(s) 607, 865, 916, 1221, 1324, 1407, 1790, 1902, 2021
BseJI GATNNNNATC 1 cut(s) 2007
BseLI CCNNNNNNNGG 7 cut(s) 253, 289, 946, 1586, 1649, 1937, 1940
BseMI GCAATG 1 cut(s) 1263
BseMII CTCAG 2 cut(s) 175, 1568
BseNI ACTGG 4 cut(s) 154, 294, 1099, 1662
BseRI GAGGAG 1 cut(s) 571
BseSI GKGCMC 2 cut(s) 156, 2085
BseXI GCAGC 9 cut(s) 56, 106, 1024, 1132, 1154, 1577, 1903, 1966, 2152
BsgI GTGCAG 2 cut(s) 663, 2060
Bsh1285I CGRYCG 1 cut(s) 33
BshFI GGCC 3 cut(s) 282, 929, 1198
BshNI GGYRCC 3 cut(s) 1158, 1523, 1927
BshTI ACCGGT 1 cut(s) 1427
BshVI ATCGAT 1 cut(s) 711
BsiEI CGRYCG 1 cut(s) 33
BsiHKAI GWGCWC 2 cut(s) 69, 162
BsiHKCI CYCGRG 2 cut(s) 50, 1907
BsiSI CCGG 6 cut(s) 62, 128, 167, 254, 279, 1428
BslFI GGGAC 2 cut(s) 1112, 1653
BslI CCNNNNNNNGG 7 cut(s) 253, 289, 946, 1586, 1649, 1937, 1940
BsmAI GTCTC 3 cut(s) 95, 251, 1904
BsmFI GGGAC 2 cut(s) 1112, 1653
BsmI GAATGC 2 cut(s) 1708, 1740
BsnI GGCC 3 cut(s) 282, 929, 1198
BsoBI CYCGRG 2 cut(s) 50, 1907
Bsp1286I GDGCHC 5 cut(s) 69, 156, 162, 953, 2085
Bsp13I TCCGGA 1 cut(s) 127
Bsp143I GATC 7 cut(s) 99, 109, 331, 604, 695, 1280, 1708
Bsp1720I GCTNAGC 2 cut(s) 161, 2169
Bsp19I CCATGG 2 cut(s) 82, 1115
BspANI GGCC 3 cut(s) 282, 929, 1198
BspCNI CTCAG 2 cut(s) 174, 1567
BspDI ATCGAT 1 cut(s) 711
BspEI TCCGGA 1 cut(s) 127
BspHI TCATGA 1 cut(s) 2121
BspMAI CTGCAG 3 cut(s) 1150, 1595, 2002
BspMI ACCTGC 2 cut(s) 755, 2005
BspPI GGATC 4 cut(s) 326, 339, 703, 1288
BspT107I GGYRCC 3 cut(s) 1158, 1523, 1927
BsrBI CCGCTC 1 cut(s) 1786
BsrDI GCAATG 1 cut(s) 1263
BsrFI RCCGGY 2 cut(s) 278, 1427
BsrI ACTGG 4 cut(s) 154, 294, 1099, 1662
BssAI RCCGGY 2 cut(s) 278, 1427
BssECI CCNNGG 7 cut(s) 82, 855, 1115, 1226, 1643, 1794, 2161
BssMI GATC 7 cut(s) 99, 109, 331, 604, 695, 1280, 1708
BssNI GRCGYC 1 cut(s) 21
BssT1I CCWWGG 4 cut(s) 82, 1115, 1226, 2161
Bst2UI CCWGG 9 cut(s) 241, 489, 507, 749, 857, 1156, 1296, 1644, 1795
Bst4CI ACNGT 5 cut(s) 31, 206, 400, 1012, 1698
Bst6I CTCTTC 2 cut(s) 1082, 2100
BstACI GRCGYC 1 cut(s) 21
BstBAI YACGTR 4 cut(s) 135, 157, 452, 772
BstC8I GCNNGC 7 cut(s) 69, 237, 280, 284, 642, 1551, 1935
BstDEI CTNAG 5 cut(s) 96, 161, 552, 1554, 2169
BstDSI CCRYGG 2 cut(s) 82, 1115
BstENI CCTNNNNNAGG 1 cut(s) 1938
BstF5I GGATG 9 cut(s) 607, 865, 916, 1221, 1324, 1407, 1790, 1902, 2021
BstH2I RGCGCY 2 cut(s) 120, 1664
BstHHI GCGC 3 cut(s) 119, 141, 1663
BstKTI GATC 7 cut(s) 102, 112, 334, 607, 698, 1283, 1711
BstMAI GTCTC 3 cut(s) 95, 251, 1904
BstMBI GATC 7 cut(s) 99, 109, 331, 604, 695, 1280, 1708
BstMCI CGRYCG 1 cut(s) 33
BstMWI GCNNNNNNNGC 5 cut(s) 41, 241, 674, 1151, 1265
BstNI CCWGG 9 cut(s) 241, 489, 507, 749, 857, 1156, 1296, 1644, 1795
BstNSI RCATGY 2 cut(s) 516, 668
BstSFI CTRYAG 5 cut(s) 735, 1053, 1146, 1591, 1998
BstSLI GKGCMC 2 cut(s) 156, 2085
BstSNI TACGTA 1 cut(s) 772
BstV1I GCAGC 9 cut(s) 56, 106, 1024, 1132, 1154, 1577, 1903, 1966, 2152
BstV2I GAAGAC 1 cut(s) 1771
BstX2I RGATCY 3 cut(s) 331, 695, 1280
BstYI RGATCY 3 cut(s) 331, 695, 1280
Bsu15I ATCGAT 1 cut(s) 711
BsuI GTATCC 1 cut(s) 1472
BsuRI GGCC 3 cut(s) 282, 929, 1198
BsuTUI ATCGAT 1 cut(s) 711
BtgI CCRYGG 2 cut(s) 82, 1115
BtgZI GCGATG 2 cut(s) 25, 88
BtsCI GGATG 9 cut(s) 607, 865, 916, 1221, 1324, 1407, 1790, 1902, 2021
BtsIMutI CAGTG 3 cut(s) 147, 1611, 1694
BveI ACCTGC 2 cut(s) 755, 2005
Cac8I GCNNGC 7 cut(s) 69, 237, 280, 284, 642, 1551, 1935
CaiI CAGNNNCTG 1 cut(s) 289
CciI TCATGA 1 cut(s) 2121
CfoI GCGC 3 cut(s) 119, 141, 1663
Cfr10I RCCGGY 2 cut(s) 278, 1427
Cfr13I GGNCC 9 cut(s) 169, 224, 1099, 1119, 1197, 1223, 1292, 1667, 1946
ClaI ATCGAT 1 cut(s) 711
CseI GACGC 1 cut(s) 29
Csp6I GTAC 6 cut(s) 7, 199, 401, 1374, 1509, 1692
CspAI ACCGGT 1 cut(s) 1427
CspCI CAANNNNNGTGG 2 cut(s) 1189, 1224
CviQI GTAC 6 cut(s) 7, 199, 401, 1374, 1509, 1692
DdeI CTNAG 5 cut(s) 96, 161, 552, 1554, 2169
DpnI GATC 7 cut(s) 101, 111, 333, 606, 697, 1282, 1710
DpnII GATC 7 cut(s) 99, 109, 331, 604, 695, 1280, 1708
DraI TTTAAA 1 cut(s) 546
EaeI YGGCCR 2 cut(s) 280, 927
Eam1104I CTCTTC 2 cut(s) 1082, 2100
EarI CTCTTC 2 cut(s) 1082, 2100
EciI GGCGGA 1 cut(s) 1959
Ecl136II GAGCTC 1 cut(s) 67
Eco105I TACGTA 1 cut(s) 772
Eco130I CCWWGG 4 cut(s) 82, 1115, 1226, 2161
Eco24I GRGCYC 2 cut(s) 69, 953
Eco47I GGWCC 8 cut(s) 169, 224, 1099, 1119, 1223, 1292, 1667, 1946
Eco53kI GAGCTC 1 cut(s) 67
Eco57I CTGAAG 1 cut(s) 1371
Eco72I CACGTG 2 cut(s) 135, 157
Eco88I CYCGRG 2 cut(s) 50, 1907
EcoICRI GAGCTC 1 cut(s) 67
EcoNI CCTNNNNNAGG 1 cut(s) 1938
EcoO109I RGGNCCY 2 cut(s) 1099, 1223
EcoRI GAATTC 2 cut(s) 1031, 1981
EcoRII CCWGG 9 cut(s) 239, 487, 505, 747, 855, 1154, 1294, 1642, 1793
EcoT14I CCWWGG 4 cut(s) 82, 1115, 1226, 2161
EcoT38I GRGCYC 2 cut(s) 69, 953
ErhI CCWWGG 4 cut(s) 82, 1115, 1226, 2161
FalI AAGNNNNNCTT 4 cut(s) 521, 553, 545, 577
FaqI GGGAC 2 cut(s) 1112, 1653
FauI CCCGC 3 cut(s) 240, 244, 291
FbaI TGATCA 1 cut(s) 1708
FblI GTMKAC 3 cut(s) 886, 1446, 2157
FokI GGATG 9 cut(s) 614, 872, 923, 1228, 1331, 1394, 1777, 1889, 2008
FriOI GRGCYC 2 cut(s) 69, 953
FspBI CTAG 5 cut(s) 342, 404, 960, 1350, 2073
GlaI GCGC 3 cut(s) 118, 140, 1662
GsuI CTGGAG 6 cut(s) 311, 486, 528, 707, 1563, 1959
HaeII RGCGCY 2 cut(s) 120, 1664
HaeIII GGCC 3 cut(s) 282, 929, 1198
HapII CCGG 6 cut(s) 62, 128, 167, 254, 279, 1428
HgaI GACGC 1 cut(s) 29
HhaI GCGC 3 cut(s) 119, 141, 1663
Hin1I GRCGYC 1 cut(s) 21
Hin6I GCGC 3 cut(s) 117, 139, 1661
HinP1I GCGC 3 cut(s) 117, 139, 1661
HinfI GANTC 7 cut(s) 78, 457, 520, 1837, 1878, 2008, 2131
HpaII CCGG 6 cut(s) 62, 128, 167, 254, 279, 1428
HphI GGTGA 5 cut(s) 887, 992, 1193, 1442, 1664
Hpy166II GTNNAC 5 cut(s) 887, 1447, 1655, 1694, 2158
Hpy188I TCNGA 5 cut(s) 224, 352, 835, 1362, 1987
Hpy8I GTNNAC 5 cut(s) 887, 1447, 1655, 1694, 2158
Hpy99I CGWCG 1 cut(s) 23
HpyAV CCTTC 7 cut(s) 187, 193, 587, 1747, 2035, 2100, 2121
HpyCH4III ACNGT 5 cut(s) 31, 206, 400, 1012, 1698
HpyCH4IV ACGT 4 cut(s) 134, 156, 451, 771
HpyCH4V TGCA 9 cut(s) 644, 662, 701, 1148, 1549, 1593, 1889, 2000, 2041
HpyF10VI GCNNNNNNNGC 5 cut(s) 41, 241, 674, 1151, 1265
HpyF3I CTNAG 5 cut(s) 96, 161, 552, 1554, 2169
HpySE526I ACGT 4 cut(s) 134, 156, 451, 771
Hsp92I GRCGYC 1 cut(s) 21
HspAI GCGC 3 cut(s) 117, 139, 1661
KflI GGGWCCC 1 cut(s) 1099
Kpn2I TCCGGA 1 cut(s) 127
KroI GCCGGC 1 cut(s) 278
KroNI GCCGGC 1 cut(s) 280
Ksp22I TGATCA 1 cut(s) 1708
Kzo9I GATC 7 cut(s) 99, 109, 331, 604, 695, 1280, 1708
LmnI GCTCC 8 cut(s) 64, 249, 509, 558, 805, 956, 1427, 1682
Lsp1109I GCAGC 9 cut(s) 56, 106, 1024, 1132, 1154, 1577, 1903, 1966, 2152
LweI GCATC 6 cut(s) 901, 1141, 1309, 1445, 1857, 2030
MaeI CTAG 5 cut(s) 342, 404, 960, 1350, 2073
MaeII ACGT 4 cut(s) 134, 156, 451, 771
MaeIII GTNAC 6 cut(s) 25, 587, 767, 980, 1327, 1799
MalI GATC 7 cut(s) 101, 111, 333, 606, 697, 1282, 1710
MbiI CCGCTC 1 cut(s) 1786
MboI GATC 7 cut(s) 99, 109, 331, 604, 695, 1280, 1708
MboII GAAGA 4 cut(s) 1069, 1776, 2056, 2087
MfeI CAATTG 1 cut(s) 1890
MflI RGATCY 3 cut(s) 331, 695, 1280
MhlI GDGCHC 5 cut(s) 69, 156, 162, 953, 2085
MlsI TGGCCA 1 cut(s) 929
MluNI TGGCCA 1 cut(s) 929
MlyI GAGTC 1 cut(s) 72
Mox20I TGGCCA 1 cut(s) 929
MroI TCCGGA 1 cut(s) 127
MroNI GCCGGC 1 cut(s) 278
MscI TGGCCA 1 cut(s) 929
MseI TTAA 8 cut(s) 269, 545, 630, 1250, 1728, 1977, 2015, 2064
MslI CAYNNNNRTG 2 cut(s) 1812, 1925
Msp20I TGGCCA 1 cut(s) 929
MspA1I CMGCKG 4 cut(s) 1145, 1916, 1957, 2036
MspI CCGG 6 cut(s) 62, 128, 167, 254, 279, 1428
MunI CAATTG 1 cut(s) 1890
Mva1269I GAATGC 2 cut(s) 1708, 1740
MvaI CCWGG 9 cut(s) 241, 489, 507, 749, 857, 1156, 1296, 1644, 1795
MwoI GCNNNNNNNGC 5 cut(s) 41, 241, 674, 1151, 1265
NaeI GCCGGC 1 cut(s) 280
NciI CCSGG 2 cut(s) 167, 254
NcoI CCATGG 2 cut(s) 82, 1115
NdeII GATC 7 cut(s) 99, 109, 331, 604, 695, 1280, 1708
NgoMIV GCCGGC 1 cut(s) 278
NmuCI GTSAC 2 cut(s) 587, 980
NspI RCATGY 2 cut(s) 516, 668
PaeR7I CTCGAG 1 cut(s) 1907
PagI TCATGA 1 cut(s) 2121
PaqCI CACCTGC 1 cut(s) 2005
PctI GAATGC 2 cut(s) 1708, 1740
PdiI GCCGGC 1 cut(s) 280
PfeI GAWTC 6 cut(s) 457, 520, 1837, 1878, 2008, 2131
PflMI CCANNNNNTGG 2 cut(s) 289, 1649
PfoI TCCNGGA 2 cut(s) 487, 505
PinAI ACCGGT 1 cut(s) 1427
PleI GAGTC 1 cut(s) 72
PmaCI CACGTG 2 cut(s) 135, 157
PmlI CACGTG 2 cut(s) 135, 157
PpsI GAGTC 1 cut(s) 72
Ppu21I YACGTR 4 cut(s) 135, 157, 452, 772
PpuMI RGGWCCY 2 cut(s) 1099, 1223
Psp124BI GAGCTC 1 cut(s) 69
Psp5II RGGWCCY 2 cut(s) 1099, 1223
Psp6I CCWGG 9 cut(s) 239, 487, 505, 747, 855, 1154, 1294, 1642, 1793
PspCI CACGTG 2 cut(s) 135, 157
PspGI CCWGG 9 cut(s) 239, 487, 505, 747, 855, 1154, 1294, 1642, 1793
PspPI GGNCC 9 cut(s) 169, 224, 1099, 1119, 1197, 1223, 1292, 1667, 1946
PspPPI RGGWCCY 2 cut(s) 1099, 1223
PstI CTGCAG 3 cut(s) 1150, 1595, 2002
PstNI CAGNNNCTG 1 cut(s) 289
PsuI RGATCY 3 cut(s) 331, 695, 1280
PvuII CAGCTG 4 cut(s) 1145, 1916, 1957, 2036
RsaI GTAC 6 cut(s) 8, 200, 402, 1375, 1510, 1693
RsaNI GTAC 6 cut(s) 7, 199, 401, 1374, 1509, 1692
RseI CAYNNNNRTG 2 cut(s) 1812, 1925
SacI GAGCTC 1 cut(s) 69
SaqAI TTAA 8 cut(s) 269, 545, 630, 1250, 1728, 1977, 2015, 2064
Sau3AI GATC 7 cut(s) 99, 109, 331, 604, 695, 1280, 1708
Sau96I GGNCC 9 cut(s) 169, 224, 1099, 1119, 1197, 1223, 1292, 1667, 1946
SchI GAGTC 1 cut(s) 72
SduI GDGCHC 5 cut(s) 69, 156, 162, 953, 2085
SfaNI GCATC 6 cut(s) 901, 1141, 1309, 1445, 1857, 2030
SfcI CTRYAG 5 cut(s) 735, 1053, 1146, 1591, 1998
Sfr274I CTCGAG 1 cut(s) 1907
SgrAI CRCCGGYG 1 cut(s) 1427
SinI GGWCC 8 cut(s) 169, 224, 1099, 1119, 1223, 1292, 1667, 1946
SlaI CTCGAG 1 cut(s) 1907
SmiMI CAYNNNNRTG 2 cut(s) 1812, 1925
SmlI CTYRAG 3 cut(s) 538, 1907, 2151
SmoI CTYRAG 3 cut(s) 538, 1907, 2151
SnaBI TACGTA 1 cut(s) 772
SspMI CTAG 5 cut(s) 342, 404, 960, 1350, 2073
SstI GAGCTC 1 cut(s) 69
StyI CCWWGG 4 cut(s) 82, 1115, 1226, 2161
TaaI ACNGT 5 cut(s) 31, 206, 400, 1012, 1698
TaiI ACGT 4 cut(s) 137, 159, 454, 774
TaqI TCGA 5 cut(s) 18, 711, 1688, 1819, 1908
TatI WGTACW 4 cut(s) 198, 400, 1373, 1691
TauI GCSGC 3 cut(s) 436, 1933, 2140
TfiI GAWTC 6 cut(s) 457, 520, 1837, 1878, 2008, 2131
Tru1I TTAA 8 cut(s) 269, 545, 630, 1250, 1728, 1977, 2015, 2064
Tru9I TTAA 8 cut(s) 269, 545, 630, 1250, 1728, 1977, 2015, 2064
TscAI CASTG 3 cut(s) 154, 1618, 1701
TseFI GTSAC 2 cut(s) 587, 980
TseI GCWGC 9 cut(s) 44, 119, 1037, 1142, 1145, 1590, 1916, 1954, 2140
Tsp45I GTSAC 2 cut(s) 587, 980
TspDTI ATGAA 5 cut(s) 428, 737, 1044, 1653, 1850
TspGWI ACGGA 1 cut(s) 1400
TspRI CASTG 3 cut(s) 154, 1618, 1701
Van91I CCANNNNNTGG 2 cut(s) 289, 1649
VpaK11BI GGWCC 8 cut(s) 169, 224, 1099, 1119, 1223, 1292, 1667, 1946
XagI CCTNNNNNAGG 1 cut(s) 1938
XapI RAATTY 5 cut(s) 547, 828, 1031, 1378, 1981
XbaI TCTAGA 1 cut(s) 2072
XceI RCATGY 2 cut(s) 516, 668
XcmI CCANNNNNNNNNTGG 2 cut(s) 387, 495
XhoI CTCGAG 1 cut(s) 1907
XmiI GTMKAC 3 cut(s) 886, 1446, 2157
XspI CTAG 5 cut(s) 342, 404, 960, 1350, 2073
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.