Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr14

Orthogroup ID

OG0010059

Physical Location & Seq

Genomic Coordinates Reverse (-)

19877419 .. 19884396

Gene Structure 6978 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom14g18140.4

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2376 bp

ATGTTTCCTCACCAGCTTAGCTCTCATTTCTCCCTCCCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTGTTTCTAATTATTGGCGACGGCGGCGGGAAGATGATGGCGTATAAGAGCCTGGACGGCGTTACAATTTCCGACATCGAAGATCTGGGGATCGAATCGGGTGCTGCGAAGAGACTCCATGGAAGTTTGACTGATATCATCGCTAATTACGGAGCTGCCACTCCGGAGACGTGGCGCAATATCACTGCGCACGTGCTCAGCCCCGAACTTCCCTTCTCGTTCCACCGGATGTTGTACTACGGCTGCTACAAGGACTTCGGACCCGACCCGCCCGCCTGGATGCCTGACCCGGAGACTGCAGGATTAACAAATGTTGGCCAGCTACTTGAGAGGAGGGGAAATGAGCTCTTGGGTTCTAGATACAAGGATCCCATATTCAGCTTTTCGGATTTTCAGGAATTTTCGGTGTCAAACCCTGAGGTTTATTGGAAAACTGTACTACTAGATGAGATGAATGCATCCTTTTATACACCTCCCCAATGCATTTTACGCGAGAATTTGTCTGGAGATGGGCATTTGTTAGTTCCTGGAGGTCAATGGCTTCCTGGAGCGTTTGGGAATCCGGCCAAAAATTGCTTGATTGTAAATCGTAAGAGGAGCTTGAATGATACCATGGTGATATGGCGCGATGAGGGAAATGATGATCTGCCTTTGAATAAGATGACGCTTAAAGAATTGCGCACAGAGGTTTGGTTAGTTGCACATGCGCTAAAAGCACTGGGTTTGGAGAAAGGCTCTGCAATCGCAATTGATATGCCGATGCATGTCAATTCCATAGTTATTTACCTGGCTATTGTTCTGGCAGGATGTGTCGTTGTATCAATCGCTGATAGTTTTGCTCCTCCTGAAATATCAACAAGGCTTAAAATATCGGATGCAAAAGCCATATTTACCCAGGATCTAATAAAACGCGGCGAGAAAAGTCTGCCCTTGTACAGTAAAATTGTGGCAGCAGAGTCTCCTATGGCAATCGTTATTCTTACCAAAGGTTCCAGTTCTAGCATGAAATTGCGTGATGGTGACATTTCCTGGCATGATTTTCTAGAAACAGTAAAAGATTTCAAAGACAATGAATTTGCTGCTGTAGAACAACCCATAGAAGCATTTACAAATATTCTCTTCTCATCTGGAACTACTGGGGATCCAAAGGCAATCCCATGGACCCTTGCAACTCCTTTCAAAGCTGCTGCAGATGCCTGGTGTCACATGGACATCCGTATAGGTGACGTTGTTGCTTGGCCCACAAATCTTGGGTGGATGATGGGCCCTTGGCTGGTATATGCTTCATTGTTAAATGGAGCTTCCATTGCCTTATATAATGGATCTCCACTTGGATCCAGCTTTGCCAAGTTTGTTCAGGATGCCAAAGTAACAATGCTTGGTGTAATCCCGAGTATTGTTCGGTCATGGAAAAGTACAAATTCTGTTTCCGGCTATGATTGGTCTGCCATTCGTTGCTTTGGATCCACCGGCGAAGCATCAAATGTAGAGGAATACCTATGGCTGATGGGGAGGGCTCACTACAAGCCTATCATTGAGTACTGTGGTGGTACAGAGATTGGTGGTGGTTTTGTTACTGGGTCGTTTTTGCAGGCTCAGTCGTTGGCTGCTTTTAGCACACCAGCTATGGGCTGCAGTTTGTATATTCTTGGCAGTGATGGTGTTCCTATTCCCCAAAATGAGCCAGGGGTTGGTGAATTGGCGCTTGATCCTATCATGTTTGGAGCTTCTAGTACATTACTGAATGCTAATCATTATGACATTTATTTCAAGGGAATGCCAATTTGGAATGGAAAGGTGTTAAGGAGGCATGGAGATGTGTTTGAACGGACATCTAGAGGTTACTATCATGCGCACGGTCGTGCAGATGATACAATGAATATTGGGGGCATCAAGGTTAGTTCGGTTGAGATTGAACGAATCTGTAATGCAGTTGATAACAATGTTCTCGAGACAGCTGCGATTGGAGTGCCGCTTGCTGGAGGCGGGCCTGAGCAGCTAGTAATTGCTGTTGTATTCAAGAACTCAGATAACCCAACTGCAGACTTGAATCAGTTGAGAATGTCCTTCAATTCAGCCGTGCAGAAGAAACTAAATCCGTTGTTCAAGGTTTCCAAAGTTGTTCCTCTCCCGTCTCTTCCAAGAACAGCAACAAATAAGGTCATGCGAAGGATTTTGCGGCAGCAGTTTGCTCAACCCGACCAAGGTGCTAAGCTTTCGCAGACTTCCCCAAGAAAAAAAAGCCATGACCTCCCTCTCCATCTCCTCCCCCAATTTCACCACCACTTTCCTCGGCCACAAACTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ACAS_N AMP-binding AMP-binding_C

Protein Analysis

792

Amino Acids

86.6

Weight (kDa)

6.39

Isoelectric Point (pI)

39.5

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010059)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G23050 AT5G23050
fragaria_vesca	FvH4_5g12960 FvH4_5g12960
malus_domestica	MD14G1218900.v1.1
prunus_persica	Prupe.5G213100_v2.0.a1 Prupe.5G213100_v2.0.a1
pyrus_communis	pycom14g18140
rosa_chinensis	RchiOBHm_Chr7g0184211
rosa_laevigata	RLG00000004993
rosa_multiflora	Rmu_sc0013812.1_g000002
rosa_roxburghii	Rroxscaffold_3G00269800
rosa_rugosa	Rorug06G0463800 Rorug06G0463800 Rorug06G0463900 Rorug06G0463900
rosa_samantha	Rh7AG081900 Rh7BG065300 Rh7CG065700 Rh7DG065400
rosa_wichuraiana	Rw7G005360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	3 cut(s)	258, 749, 1923
AccB7I	CCANNNNNTGG	1 cut(s)	1760
AccII	CGCG	3 cut(s)	561, 696, 981
AccIII	TCCGGA	1 cut(s)	232
AciI	CCGC	8 cut(s)	93, 96, 338, 342, 981, 2042, 2055, 2248
AcoI	YGGCCR	3 cut(s)	385, 633, 2363
AcsI	RAATTY	4 cut(s)	467, 565, 1142, 1489
AcvI	CACGTG	1 cut(s)	262
AfaI	GTAC	7 cut(s)	305, 507, 1004, 1486, 1610, 1621, 1804
AfiI	CCNNNNNNNGG	5 cut(s)	1342, 1697, 1760, 2048, 2273
AjiI	CACGTC	1 cut(s)	240
AjnI	CCWGG	9 cut(s)	120, 344, 595, 613, 855, 963, 1097, 1265, 1753
AjuI	GAANNNNNNNTTGG	2 cut(s)	1837, 1869
AleI	CACNNNNGTG	1 cut(s)	1929
Alw21I	GWGCWC	2 cut(s)	267, 417
Alw26I	GTCTC	6 cut(s)	175, 230, 356, 1032, 2015, 2208
Ama87I	CYCGRG	2 cut(s)	1459, 2018
Aor13HI	TCCGGA	1 cut(s)	232
AoxI	GGCC	6 cut(s)	385, 633, 1307, 1333, 2057, 2363
ApaI	GGGCCC	1 cut(s)	1337
ApoI	RAATTY	4 cut(s)	467, 565, 1142, 1489
ArsI	GACNNNNNNTTYG	2 cut(s)	1127, 1159
AspLEI	GCGC	7 cut(s)	246, 259, 696, 750, 778, 1774, 1924
AspS9I	GGNCC	6 cut(s)	329, 1230, 1308, 1333, 1334, 2057
AsuC2I	CCSGG	1 cut(s)	359
AsuHPI	GGTGA	5 cut(s)	697, 1100, 1304, 1775, 2339
AvaI	CYCGRG	2 cut(s)	1459, 2018
AvaII	GGWCC	2 cut(s)	329, 1230
AxyI	CCTNAGG	1 cut(s)	486
BaeGI	GKGCMC	1 cut(s)	1337
BaeI	ACNNNNGTAYC	2 cut(s)	669, 702
BalI	TGGCCA	1 cut(s)	387
BamHI	GGATCC	4 cut(s)	436, 1210, 1403, 1532
BanII	GRGCYC	3 cut(s)	417, 1337, 1588
BbrPI	CACGTG	1 cut(s)	262
Bbv12I	GWGCWC	2 cut(s)	267, 417
BccI	CCATC	7 cut(s)	100, 572, 1079, 1324, 1570, 1721, 2337
BceAI	ACGGC	4 cut(s)	106, 142, 325, 2132
BcgI	CGANNNNNNTGC	8 cut(s)	152, 186, 2009, 2020, 2043, 2054, 2258, 2292
BciT130I	CCWGG	9 cut(s)	122, 346, 597, 615, 857, 965, 1099, 1267, 1755
BcnI	CCSGG	1 cut(s)	359
BcoDI	GTCTC	6 cut(s)	175, 230, 356, 1032, 2015, 2208
BfaI	CTAG	7 cut(s)	426, 512, 1068, 1112, 1800, 1905, 2069
BfmI	CTRYAG	5 cut(s)	366, 1152, 1257, 1702, 2109
BfoI	RGCGCY	1 cut(s)	1775
BglI	GCCNNNNNGGC	1 cut(s)	126
BglII	AGATCT	1 cut(s)	151
BlpI	GCTNAGC	3 cut(s)	17, 266, 2280
BmcAI	AGTACT	1 cut(s)	1610
Bme18I	GGWCC	2 cut(s)	329, 1230
BmeT110I	CYCGRG	2 cut(s)	1459, 2018
BmgBI	CACGTC	1 cut(s)	240
BmgT120I	GGNCC	6 cut(s)	329, 1230, 1308, 1333, 1334, 2057
BmiI	GGNNCC	8 cut(s)	331, 438, 1060, 1212, 1232, 1335, 1405, 1534
BmrI	ACTGGG	3 cut(s)	797, 1215, 1656
BmsI	GCATC	8 cut(s)	339, 536, 819, 934, 1252, 1420, 1556, 1968
BmuI	ACTGGG	3 cut(s)	797, 1215, 1656
BplI	GAGNNNNNCTC	2 cut(s)	788, 820
BpmI	CTGGAG	4 cut(s)	594, 618, 636, 2070
Bpu10I	CCTNAGC	1 cut(s)	2061
Bpu1102I	GCTNAGC	3 cut(s)	17, 266, 2280
BpuEI	CTTGAG	1 cut(s)	416
BpuMI	CCSGG	1 cut(s)	359
BsaAI	YACGTR	1 cut(s)	262
BsaJI	CCNNGG	8 cut(s)	187, 681, 963, 1226, 1337, 1754, 2272, 2360
BsaWI	WCCGGW	2 cut(s)	232, 294
Bsc4I	CCNNNNNNNGG	5 cut(s)	1342, 1697, 1760, 2048, 2273
Bse118I	RCCGGY	1 cut(s)	1538
Bse1I	ACTGG	4 cut(s)	792, 1062, 1210, 1651
Bse21I	CCTNAGG	1 cut(s)	486
Bse3DI	GCAATG	1 cut(s)	1374
BseAI	TCCGGA	1 cut(s)	232
BseBI	CCWGG	9 cut(s)	122, 346, 597, 615, 857, 965, 1099, 1267, 1755
BseDI	CCNNGG	8 cut(s)	187, 681, 963, 1226, 1337, 1754, 2272, 2360
BseGI	GGATG	8 cut(s)	303, 354, 527, 881, 949, 1281, 1332, 1435
BseLI	CCNNNNNNNGG	5 cut(s)	1342, 1697, 1760, 2048, 2273
BseMI	GCAATG	1 cut(s)	1374
BseMII	CTCAG	5 cut(s)	280, 477, 1679, 2052, 2109
BseNI	ACTGG	4 cut(s)	792, 1062, 1210, 1651
BseRI	GAGGAG	4 cut(s)	415, 679, 900, 2324
BseSI	GKGCMC	1 cut(s)	1337
BsgI	GTGCAG	2 cut(s)	1953, 2171
Bsh1236I	CGCG	3 cut(s)	561, 696, 981
Bsh1285I	CGRYCG	1 cut(s)	1930
BshFI	GGCC	6 cut(s)	387, 635, 1309, 1335, 2059, 2365
BsiEI	CGRYCG	1 cut(s)	1930
BsiHKAI	GWGCWC	2 cut(s)	267, 417
BsiHKCI	CYCGRG	2 cut(s)	1459, 2018
BsiSI	CCGG	6 cut(s)	233, 295, 359, 632, 1500, 1539
BslI	CCNNNNNNNGG	5 cut(s)	1342, 1697, 1760, 2048, 2273
BsmAI	GTCTC	6 cut(s)	175, 230, 356, 1032, 2015, 2208
BsmBI	CGTCTC	2 cut(s)	230, 2208
BsmI	GAATGC	3 cut(s)	529, 1819, 1851
BsnI	GGCC	6 cut(s)	387, 635, 1309, 1335, 2059, 2365
BsoBI	CYCGRG	2 cut(s)	1459, 2018
Bsp120I	GGGCCC	1 cut(s)	1333
Bsp1286I	GDGCHC	4 cut(s)	267, 417, 1337, 1588
Bsp13I	TCCGGA	1 cut(s)	232
Bsp1407I	TGTACA	1 cut(s)	1002
Bsp1720I	GCTNAGC	3 cut(s)	17, 266, 2280
Bsp19I	CCATGG	3 cut(s)	187, 681, 1226
BspACI	CCGC	8 cut(s)	93, 96, 338, 342, 981, 2042, 2055, 2248
BspANI	GGCC	6 cut(s)	387, 635, 1309, 1335, 2059, 2365
BspCNI	CTCAG	5 cut(s)	279, 478, 1678, 2053, 2108
BspEI	TCCGGA	1 cut(s)	232
BspFNI	CGCG	3 cut(s)	561, 696, 981
BspLI	GGNNCC	8 cut(s)	331, 438, 1060, 1212, 1232, 1335, 1405, 1534
BspMAI	CTGCAG	4 cut(s)	370, 1261, 1706, 2113
BsrDI	GCAATG	1 cut(s)	1374
BsrFI	RCCGGY	1 cut(s)	1538
BsrGI	TGTACA	1 cut(s)	1002
BsrI	ACTGG	4 cut(s)	792, 1062, 1210, 1651
BssAI	RCCGGY	1 cut(s)	1538
BssECI	CCNNGG	8 cut(s)	187, 681, 963, 1226, 1337, 1754, 2272, 2360
BssT1I	CCWWGG	5 cut(s)	187, 681, 1226, 1337, 2272
Bst2UI	CCWGG	9 cut(s)	122, 346, 597, 615, 857, 965, 1099, 1267, 1755
Bst4CI	ACNGT	5 cut(s)	505, 1007, 1120, 1613, 1928
Bst6I	CTCTTC	3 cut(s)	173, 1193, 2211
BstAUI	TGTACA	1 cut(s)	1002
BstBAI	YACGTR	1 cut(s)	262
BstC8I	GCNNGC	5 cut(s)	342, 389, 1662, 2046, 2057
BstDEI	CTNAG	7 cut(s)	17, 266, 486, 1665, 2061, 2095, 2280
BstDSI	CCRYGG	3 cut(s)	187, 681, 1226
BstF5I	GGATG	8 cut(s)	303, 354, 527, 881, 949, 1281, 1332, 1435
BstFNI	CGCG	3 cut(s)	561, 696, 981
BstH2I	RGCGCY	1 cut(s)	1775
BstHHI	GCGC	7 cut(s)	246, 259, 696, 750, 778, 1774, 1924
BstMAI	GTCTC	6 cut(s)	175, 230, 356, 1032, 2015, 2208
BstMCI	CGRYCG	1 cut(s)	1930
BstMWI	GCNNNNNNNGC	7 cut(s)	93, 126, 558, 782, 1262, 1376, 2065
BstNI	CCWGG	9 cut(s)	122, 346, 597, 615, 857, 965, 1099, 1267, 1755
BstNSI	RCATGY	2 cut(s)	776, 836
BstSFI	CTRYAG	5 cut(s)	366, 1152, 1257, 1702, 2109
BstSLI	GKGCMC	1 cut(s)	1337
BstUI	CGCG	3 cut(s)	561, 696, 981
BstX2I	RGATCY	7 cut(s)	151, 436, 967, 1210, 1391, 1403, 1532
BstYI	RGATCY	7 cut(s)	151, 436, 967, 1210, 1391, 1403, 1532
Bsu36I	CCTNAGG	1 cut(s)	486
BsuRI	GGCC	6 cut(s)	387, 635, 1309, 1335, 2059, 2365
BtgI	CCRYGG	3 cut(s)	187, 681, 1226
BtgZI	GCGATG	2 cut(s)	193, 711
BtrI	CACGTC	1 cut(s)	240
BtsCI	GGATG	8 cut(s)	303, 354, 527, 881, 949, 1281, 1332, 1435
BtsI	GCAGTG	2 cut(s)	252, 1729
BtsIMutI	CAGTG	3 cut(s)	252, 785, 1729
Cac8I	GCNNGC	5 cut(s)	342, 389, 1662, 2046, 2057
CfoI	GCGC	7 cut(s)	246, 259, 696, 750, 778, 1774, 1924
Cfr10I	RCCGGY	1 cut(s)	1538
Cfr13I	GGNCC	6 cut(s)	329, 1230, 1308, 1333, 1334, 2057
CseI	GACGC	1 cut(s)	742
Csp6I	GTAC	7 cut(s)	304, 506, 1003, 1485, 1609, 1620, 1803
CspCI	CAANNNNNGTGG	2 cut(s)	1300, 1335
CviQI	GTAC	7 cut(s)	304, 506, 1003, 1485, 1609, 1620, 1803
DdeI	CTNAG	7 cut(s)	17, 266, 486, 1665, 2061, 2095, 2280
EaeI	YGGCCR	3 cut(s)	385, 633, 2363
Eam1104I	CTCTTC	3 cut(s)	173, 1193, 2211
EarI	CTCTTC	3 cut(s)	173, 1193, 2211
Ecl136II	GAGCTC	1 cut(s)	415
Eco130I	CCWWGG	5 cut(s)	187, 681, 1226, 1337, 2272
Eco24I	GRGCYC	3 cut(s)	417, 1337, 1588
Eco32I	GATATC	1 cut(s)	205
Eco47I	GGWCC	2 cut(s)	329, 1230
Eco53kI	GAGCTC	1 cut(s)	415
Eco72I	CACGTG	1 cut(s)	262
Eco81I	CCTNAGG	1 cut(s)	486
Eco88I	CYCGRG	2 cut(s)	1459, 2018
EcoICRI	GAGCTC	1 cut(s)	415
EcoO109I	RGGNCCY	1 cut(s)	1334
EcoRII	CCWGG	9 cut(s)	120, 344, 595, 613, 855, 963, 1097, 1265, 1753
EcoRV	GATATC	1 cut(s)	205
EcoT14I	CCWWGG	5 cut(s)	187, 681, 1226, 1337, 2272
EcoT22I	ATGCAT	3 cut(s)	529, 554, 834
EcoT38I	GRGCYC	3 cut(s)	417, 1337, 1588
ErhI	CCWWGG	5 cut(s)	187, 681, 1226, 1337, 2272
Esp3I	CGTCTC	2 cut(s)	230, 2208
FalI	AAGNNNNNCTT	2 cut(s)	653, 685
FauI	CCCGC	4 cut(s)	89, 345, 349, 2048
FokI	GGATG	8 cut(s)	310, 361, 514, 888, 956, 1268, 1339, 1442
FriOI	GRGCYC	3 cut(s)	417, 1337, 1588
FspAI	RTGCGCAY	1 cut(s)	1923
FspBI	CTAG	7 cut(s)	426, 512, 1068, 1112, 1800, 1905, 2069
FspI	TGCGCA	3 cut(s)	258, 749, 1923
GlaI	GCGC	7 cut(s)	245, 258, 695, 749, 777, 1773, 1923
GsuI	CTGGAG	4 cut(s)	594, 618, 636, 2070
HaeII	RGCGCY	1 cut(s)	1775
HaeIII	GGCC	6 cut(s)	387, 635, 1309, 1335, 2059, 2365
HapII	CCGG	6 cut(s)	233, 295, 359, 632, 1500, 1539
HgaI	GACGC	1 cut(s)	742
HhaI	GCGC	7 cut(s)	246, 259, 696, 750, 778, 1774, 1924
Hin6I	GCGC	7 cut(s)	244, 257, 694, 748, 776, 1772, 1922
HinP1I	GCGC	7 cut(s)	244, 257, 694, 748, 776, 1772, 1922
HindIII	AAGCTT	1 cut(s)	2282
HinfI	GANTC	6 cut(s)	164, 183, 628, 1025, 1989, 2119
HpaII	CCGG	6 cut(s)	233, 295, 359, 632, 1500, 1539
HphI	GGTGA	5 cut(s)	697, 1100, 1304, 1775, 2339
Hpy188I	TCNGA	5 cut(s)	142, 329, 457, 943, 2098
Hpy99I	CGWCG	1 cut(s)	92
HpyAV	CCTTC	3 cut(s)	292, 2146, 2232
HpyCH4III	ACNGT	5 cut(s)	505, 1007, 1120, 1613, 1928
HpyCH4IV	ACGT	3 cut(s)	239, 261, 1296
HpyF10VI	GCNNNNNNNGC	7 cut(s)	93, 126, 558, 782, 1262, 1376, 2065
HpyF3I	CTNAG	7 cut(s)	17, 266, 486, 1665, 2061, 2095, 2280
HpySE526I	ACGT	3 cut(s)	239, 261, 1296
HspAI	GCGC	7 cut(s)	244, 257, 694, 748, 776, 1772, 1922
Kpn2I	TCCGGA	1 cut(s)	232
LmnI	GCTCC	6 cut(s)	221, 617, 666, 913, 1367, 1793
LweI	GCATC	8 cut(s)	339, 536, 819, 934, 1252, 1420, 1556, 1968
MaeI	CTAG	7 cut(s)	426, 512, 1068, 1112, 1800, 1905, 2069
MaeII	ACGT	3 cut(s)	239, 261, 1296
MaeIII	GTNAC	7 cut(s)	130, 1088, 1271, 1292, 1438, 1642, 1910
MboII	GAAGA	6 cut(s)	112, 161, 190, 1180, 2167, 2198
MfeI	CAATTG	1 cut(s)	816
MflI	RGATCY	7 cut(s)	151, 436, 967, 1210, 1391, 1403, 1532
MhlI	GDGCHC	4 cut(s)	267, 417, 1337, 1588
MlsI	TGGCCA	1 cut(s)	387
MluNI	TGGCCA	1 cut(s)	387
MlyI	GAGTC	2 cut(s)	177, 1034
MmeI	TCCRAC	1 cut(s)	165
Mox20I	TGGCCA	1 cut(s)	387
Mph1103I	ATGCAT	3 cut(s)	529, 554, 834
MroI	TCCGGA	1 cut(s)	232
MscI	TGGCCA	1 cut(s)	387
MseI	TTAA	6 cut(s)	374, 738, 933, 1361, 1871, 2374
MslI	CAYNNNNRTG	2 cut(s)	1884, 1929
Msp20I	TGGCCA	1 cut(s)	387
MspA1I	CMGCKG	1 cut(s)	2027
MspI	CCGG	6 cut(s)	233, 295, 359, 632, 1500, 1539
MunI	CAATTG	1 cut(s)	816
Mva1269I	GAATGC	3 cut(s)	529, 1819, 1851
MvaI	CCWGG	9 cut(s)	122, 346, 597, 615, 857, 965, 1099, 1267, 1755
MvnI	CGCG	3 cut(s)	561, 696, 981
MwoI	GCNNNNNNNGC	7 cut(s)	93, 126, 558, 782, 1262, 1376, 2065
NciI	CCSGG	1 cut(s)	359
NcoI	CCATGG	3 cut(s)	187, 681, 1226
NlaIV	GGNNCC	8 cut(s)	331, 438, 1060, 1212, 1232, 1335, 1405, 1534
NmeAIII	GCCGAG	1 cut(s)	2341
NmuCI	GTSAC	3 cut(s)	1088, 1271, 1292
NsbI	TGCGCA	3 cut(s)	258, 749, 1923
NsiI	ATGCAT	3 cut(s)	529, 554, 834
NspI	RCATGY	2 cut(s)	776, 836
OliI	CACNNNNGTG	1 cut(s)	1929
PaeR7I	CTCGAG	1 cut(s)	2018
PcsI	WCGNNNNNNNCGW	1 cut(s)	173
PctI	GAATGC	3 cut(s)	529, 1819, 1851
PfeI	GAWTC	4 cut(s)	164, 628, 1989, 2119
PflMI	CCANNNNNTGG	1 cut(s)	1760
PfoI	TCCNGGA	2 cut(s)	595, 613
PleI	GAGTC	2 cut(s)	177, 1033
PmaCI	CACGTG	1 cut(s)	262
PmlI	CACGTG	1 cut(s)	262
PpsI	GAGTC	2 cut(s)	177, 1033
Ppu21I	YACGTR	1 cut(s)	262
Psp124BI	GAGCTC	1 cut(s)	417
Psp6I	CCWGG	9 cut(s)	120, 344, 595, 613, 855, 963, 1097, 1265, 1753
PspCI	CACGTG	1 cut(s)	262
PspGI	CCWGG	9 cut(s)	120, 344, 595, 613, 855, 963, 1097, 1265, 1753
PspN4I	GGNNCC	8 cut(s)	331, 438, 1060, 1212, 1232, 1335, 1405, 1534
PspOMI	GGGCCC	1 cut(s)	1333
PspPI	GGNCC	6 cut(s)	329, 1230, 1308, 1333, 1334, 2057
PstI	CTGCAG	4 cut(s)	370, 1261, 1706, 2113
PsuI	RGATCY	7 cut(s)	151, 436, 967, 1210, 1391, 1403, 1532
PvuII	CAGCTG	1 cut(s)	2027
RsaI	GTAC	7 cut(s)	305, 507, 1004, 1486, 1610, 1621, 1804
RsaNI	GTAC	7 cut(s)	304, 506, 1003, 1485, 1609, 1620, 1803
RseI	CAYNNNNRTG	2 cut(s)	1884, 1929
SacI	GAGCTC	1 cut(s)	417
SaqAI	TTAA	6 cut(s)	374, 738, 933, 1361, 1871, 2374
Sau96I	GGNCC	6 cut(s)	329, 1230, 1308, 1333, 1334, 2057
ScaI	AGTACT	1 cut(s)	1610
SchI	GAGTC	2 cut(s)	177, 1034
SduI	GDGCHC	4 cut(s)	267, 417, 1337, 1588
SfaNI	GCATC	8 cut(s)	339, 536, 819, 934, 1252, 1420, 1556, 1968
SfcI	CTRYAG	5 cut(s)	366, 1152, 1257, 1702, 2109
Sfr274I	CTCGAG	1 cut(s)	2018
SgrAI	CRCCGGYG	1 cut(s)	1538
SinI	GGWCC	2 cut(s)	329, 1230
SlaI	CTCGAG	1 cut(s)	2018
SmiMI	CAYNNNNRTG	2 cut(s)	1884, 1929
SmlI	CTYRAG	2 cut(s)	395, 2018
SmoI	CTYRAG	2 cut(s)	395, 2018
SsiI	CCGC	8 cut(s)	93, 96, 338, 342, 981, 2042, 2055, 2248
SspI	AATATT	2 cut(s)	1183, 1951
SspMI	CTAG	7 cut(s)	426, 512, 1068, 1112, 1800, 1905, 2069
SstI	GAGCTC	1 cut(s)	417
StyI	CCWWGG	5 cut(s)	187, 681, 1226, 1337, 2272
TaaI	ACNGT	5 cut(s)	505, 1007, 1120, 1613, 1928
TaiI	ACGT	3 cut(s)	242, 264, 1299
TaqI	TCGA	3 cut(s)	147, 162, 2019
TaqII	GACCGA	1 cut(s)	1461
TatI	WGTACW	6 cut(s)	303, 505, 1002, 1484, 1608, 1802
TauI	GCSGC	4 cut(s)	96, 984, 2044, 2251
TfiI	GAWTC	4 cut(s)	164, 628, 1989, 2119
Tru1I	TTAA	6 cut(s)	374, 738, 933, 1361, 1871, 2374
Tru9I	TTAA	6 cut(s)	374, 738, 933, 1361, 1871, 2374
TscAI	CASTG	3 cut(s)	259, 792, 1729
TseFI	GTSAC	3 cut(s)	1088, 1271, 1292
Tsp45I	GTSAC	3 cut(s)	1088, 1271, 1292
TspDTI	ATGAA	5 cut(s)	536, 1088, 1155, 1344, 1961
TspGWI	ACGGA	4 cut(s)	234, 1274, 1912, 2157
TspRI	CASTG	3 cut(s)	259, 792, 1729
Van91I	CCANNNNNTGG	1 cut(s)	1760
VpaK11BI	GGWCC	2 cut(s)	329, 1230
XapI	RAATTY	4 cut(s)	467, 565, 1142, 1489
XbaI	TCTAGA	3 cut(s)	425, 1111, 1904
XceI	RCATGY	2 cut(s)	776, 836
XhoI	CTCGAG	1 cut(s)	2018
XspI	CTAG	7 cut(s)	426, 512, 1068, 1112, 1800, 1905, 2069
ZrmI	AGTACT	1 cut(s)	1610
Zsp2I	ATGCAT	3 cut(s)	529, 554, 834

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.