MD14G1219300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0016706
Physical Location & Seq
Forward (+)
30190396 .. 30190815
420 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1219300.v1.1.491

Sequence Viewer

Length: 420 bp
ATGGAAACAACATCACAAACATTGCCTGGTTCAGCGGGCGATCAAATCTGGGGTGGTGGTGGTAGCAGTAATAATATTCCCCAAGCAGTACCCTTAGTTTCTTCTTCTTCTTCGTCTTCGGGTTCCATTGGGCCATTCTTTGCAGTGATCTCCGTCCTAACCATCCTCGCAGTTCTGTCGTGCTTTTTGGGCCGGAGGCTGACTCGTGATCAGATGGTACCGACTCCGGTGGAGAGCATCAGGGATAGAATCGATTGTTTCGGGTGGTTGAAGCGCAAGTGCCGGCAGCTGCAGTGCATGGTTGCTCATCATGACCTCGAAGTTGGATCAAAGGTGATGGTCATGGATCTTAGTCAAGAAAAGAAAGGTGATGCTAGGGTTAAAGAAGGTGACCATGAAGTTCCTCCCCAGAAGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

140

Amino Acids

14.98

Weight (kDa)

7.7

Isoelectric Point (pI)

56.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016706)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G74055
malus_domestica MD14G1219300.v1.1
prunus_persica Prupe.5G214000_v2.0.a1
pyrus_communis pycom06g18650 pycom14g18170
rosa_chinensis RchiOBHm_Chr7g0184281
rosa_laevigata RLG00000004990
rosa_multiflora Rmu_co8406705.1_g000001
rosa_roxburghii Rroxscaffold_3G00269770
rosa_samantha Rh7AG081500 Rh7BG065800 Rh7CG066100 Rh7DG065800
rosa_wichuraiana Rw7G005390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 217
AccB1I GGYRCC 1 cut(s) 217
AciI CCGC 1 cut(s) 35
AclWI GGATC 2 cut(s) 334, 354
AfaI GTAC 2 cut(s) 90, 219
AgsI TTSAA 1 cut(s) 271
AjnI CCWGG 1 cut(s) 25
AluBI AGCT 1 cut(s) 289
AluI AGCT 1 cut(s) 289
AlwI GGATC 2 cut(s) 334, 354
AoxI GGCC 2 cut(s) 131, 190
ApeKI GCWGC 2 cut(s) 286, 289
Asp718I GGTACC 1 cut(s) 217
AspLEI GCGC 1 cut(s) 276
AspS9I GGNCC 2 cut(s) 131, 190
AsuHPI GGTGA 3 cut(s) 346, 380, 401
BanI GGYRCC 1 cut(s) 217
BauI CACGAG 1 cut(s) 204
BbsI GAAGAC 1 cut(s) 108
BbvI GCAGC 2 cut(s) 276, 298
BccI CCATC 3 cut(s) 170, 208, 331
BcgI CGANNNNNNTGC 2 cut(s) 159, 193
BciT130I CCWGG 1 cut(s) 27
BclI TGATCA 1 cut(s) 208
BfaI CTAG 1 cut(s) 375
BfmI CTRYAG 1 cut(s) 290
BisI GCNGC 2 cut(s) 287, 290
BlsI GCNGC 2 cut(s) 288, 291
Bme1390I CCNGG 1 cut(s) 27
BmgT120I GGNCC 2 cut(s) 131, 190
BmiI GGNNCC 2 cut(s) 124, 219
BmrFI CCNGG 1 cut(s) 27
BmsI GCATC 2 cut(s) 246, 361
BpiI GAAGAC 1 cut(s) 108
BplI GAGNNNNNCTC 2 cut(s) 187, 219
Bsa29I ATCGAT 1 cut(s) 252
BsaWI WCCGGW 1 cut(s) 226
Bse118I RCCGGY 1 cut(s) 282
Bse3DI GCAATG 1 cut(s) 20
BseBI CCWGG 1 cut(s) 27
BseCI ATCGAT 1 cut(s) 252
BseGI GGATG 1 cut(s) 162
BseMI GCAATG 1 cut(s) 20
BseXI GCAGC 2 cut(s) 276, 298
BshFI GGCC 2 cut(s) 133, 192
BshNI GGYRCC 1 cut(s) 217
BshVI ATCGAT 1 cut(s) 252
BsiSI CCGG 3 cut(s) 193, 227, 283
BsnI GGCC 2 cut(s) 133, 192
Bsp143I GATC 5 cut(s) 40, 147, 208, 326, 346
BspACI CCGC 1 cut(s) 35
BspANI GGCC 2 cut(s) 133, 192
BspDI ATCGAT 1 cut(s) 252
BspHI TCATGA 1 cut(s) 310
BspLI GGNNCC 2 cut(s) 124, 219
BspMAI CTGCAG 1 cut(s) 294
BspPI GGATC 2 cut(s) 334, 354
BspT107I GGYRCC 1 cut(s) 217
BsrDI GCAATG 1 cut(s) 20
BsrFI RCCGGY 1 cut(s) 282
BssAI RCCGGY 1 cut(s) 282
BssMI GATC 5 cut(s) 40, 147, 208, 326, 346
BssSI CACGAG 1 cut(s) 204
Bst2BI CACGAG 1 cut(s) 204
Bst2UI CCWGG 1 cut(s) 27
BstC8I GCNNGC 2 cut(s) 37, 284
BstDEI CTNAG 2 cut(s) 94, 350
BstEII GGTNACC 1 cut(s) 389
BstF5I GGATG 1 cut(s) 162
BstHHI GCGC 1 cut(s) 276
BstKTI GATC 5 cut(s) 43, 150, 211, 329, 349
BstMBI GATC 5 cut(s) 40, 147, 208, 326, 346
BstMWI GCNNNNNNNGC 1 cut(s) 189
BstNI CCWGG 1 cut(s) 27
BstPI GGTNACC 1 cut(s) 389
BstSCI CCNGG 1 cut(s) 25
BstSFI CTRYAG 1 cut(s) 290
BstV1I GCAGC 2 cut(s) 276, 298
BstV2I GAAGAC 1 cut(s) 108
BstX2I RGATCY 1 cut(s) 346
BstYI RGATCY 1 cut(s) 346
Bsu15I ATCGAT 1 cut(s) 252
BsuRI GGCC 2 cut(s) 133, 192
BsuTUI ATCGAT 1 cut(s) 252
BtsCI GGATG 1 cut(s) 162
BtsI GCAGTG 2 cut(s) 150, 299
BtsIMutI CAGTG 2 cut(s) 150, 299
Cac8I GCNNGC 2 cut(s) 37, 284
CciI TCATGA 1 cut(s) 310
CfoI GCGC 1 cut(s) 276
Cfr10I RCCGGY 1 cut(s) 282
Cfr13I GGNCC 2 cut(s) 131, 190
ClaI ATCGAT 1 cut(s) 252
Csp6I GTAC 2 cut(s) 89, 218
CviAII CATG 4 cut(s) 298, 311, 343, 395
CviJI RGCY 5 cut(s) 133, 192, 199, 289, 416
CviKI_1 RGCY 5 cut(s) 133, 192, 199, 289, 416
CviQI GTAC 2 cut(s) 89, 218
DdeI CTNAG 2 cut(s) 94, 350
DpnI GATC 5 cut(s) 42, 149, 210, 328, 348
DpnII GATC 5 cut(s) 40, 147, 208, 326, 346
Eco91I GGTNACC 1 cut(s) 389
EcoO65I GGTNACC 1 cut(s) 389
EcoRII CCWGG 1 cut(s) 25
FaeI CATG 4 cut(s) 301, 314, 346, 398
FaiI YATR 4 cut(s) 299, 312, 344, 396
FatI CATG 4 cut(s) 297, 310, 342, 394
FauI CCCGC 1 cut(s) 28
FbaI TGATCA 1 cut(s) 208
Fnu4HI GCNGC 2 cut(s) 287, 290
FokI GGATG 1 cut(s) 149
Fsp4HI GCNGC 2 cut(s) 287, 290
FspBI CTAG 1 cut(s) 375
GlaI GCGC 1 cut(s) 275
GluI GCNGC 2 cut(s) 287, 290
HaeIII GGCC 2 cut(s) 133, 192
HapII CCGG 3 cut(s) 193, 227, 283
HhaI GCGC 1 cut(s) 276
Hin1II CATG 4 cut(s) 301, 314, 346, 398
Hin6I GCGC 1 cut(s) 274
HinP1I GCGC 1 cut(s) 274
HinfI GANTC 3 cut(s) 202, 223, 249
HpaII CCGG 3 cut(s) 193, 227, 283
HphI GGTGA 3 cut(s) 346, 380, 401
Hpy188I TCNGA 1 cut(s) 213
Hpy188III TCNNGA 3 cut(s) 206, 311, 356
HpyAV CCTTC 2 cut(s) 380, 406
HpyCH4V TGCA 3 cut(s) 143, 292, 297
HpyF10VI GCNNNNNNNGC 1 cut(s) 189
HpyF3I CTNAG 2 cut(s) 94, 350
Hsp92II CATG 4 cut(s) 301, 314, 346, 398
HspAI GCGC 1 cut(s) 274
KpnI GGTACC 1 cut(s) 221
KroI GCCGGC 1 cut(s) 282
KroNI GCCGGC 1 cut(s) 284
Ksp22I TGATCA 1 cut(s) 208
Kzo9I GATC 5 cut(s) 40, 147, 208, 326, 346
LpnPI CCDG 7 cut(s) 12, 34, 39, 206, 226, 240, 296
Lsp1109I GCAGC 2 cut(s) 276, 298
LweI GCATC 2 cut(s) 246, 361
MaeI CTAG 1 cut(s) 375
MaeIII GTNAC 1 cut(s) 389
MalI GATC 5 cut(s) 42, 149, 210, 328, 348
MboI GATC 5 cut(s) 40, 147, 208, 326, 346
MboII GAAGA 5 cut(s) 93, 96, 99, 102, 108
MflI RGATCY 1 cut(s) 346
MlyI GAGTC 2 cut(s) 196, 217
MmeI TCCRAC 1 cut(s) 304
MnlI CCTC 4 cut(s) 176, 189, 326, 414
MroNI GCCGGC 1 cut(s) 282
MseI TTAA 1 cut(s) 381
MspA1I CMGCKG 2 cut(s) 35, 289
MspI CCGG 3 cut(s) 193, 227, 283
MspR9I CCNGG 1 cut(s) 27
MvaI CCWGG 1 cut(s) 27
MwoI GCNNNNNNNGC 1 cut(s) 189
NaeI GCCGGC 1 cut(s) 284
NdeII GATC 5 cut(s) 40, 147, 208, 326, 346
NgoMIV GCCGGC 1 cut(s) 282
NlaIII CATG 4 cut(s) 301, 314, 346, 398
NlaIV GGNNCC 2 cut(s) 124, 219
NmuCI GTSAC 1 cut(s) 389
PagI TCATGA 1 cut(s) 310
PdiI GCCGGC 1 cut(s) 284
PfeI GAWTC 1 cut(s) 249
PkrI GCNGC 2 cut(s) 288, 291
PleI GAGTC 2 cut(s) 196, 217
PpsI GAGTC 2 cut(s) 196, 217
Psp6I CCWGG 1 cut(s) 25
PspEI GGTNACC 1 cut(s) 389
PspGI CCWGG 1 cut(s) 25
PspN4I GGNNCC 2 cut(s) 124, 219
PspPI GGNCC 2 cut(s) 131, 190
PstI CTGCAG 1 cut(s) 294
PsuI RGATCY 1 cut(s) 346
PvuII CAGCTG 1 cut(s) 289
RsaI GTAC 2 cut(s) 90, 219
RsaNI GTAC 2 cut(s) 89, 218
SaqAI TTAA 1 cut(s) 381
SatI GCNGC 2 cut(s) 287, 290
Sau3AI GATC 5 cut(s) 40, 147, 208, 326, 346
Sau96I GGNCC 2 cut(s) 131, 190
SchI GAGTC 2 cut(s) 196, 217
ScrFI CCNGG 1 cut(s) 27
SetI ASST 5 cut(s) 291, 318, 336, 370, 391
SfaNI GCATC 2 cut(s) 246, 361
SfcI CTRYAG 1 cut(s) 290
SsiI CCGC 1 cut(s) 35
SspI AATATT 1 cut(s) 76
SspMI CTAG 1 cut(s) 375
StyD4I CCNGG 1 cut(s) 25
TaqI TCGA 2 cut(s) 252, 318
TfiI GAWTC 1 cut(s) 249
Tru1I TTAA 1 cut(s) 381
Tru9I TTAA 1 cut(s) 381
TscAI CASTG 2 cut(s) 150, 299
TseFI GTSAC 1 cut(s) 389
TseI GCWGC 2 cut(s) 286, 289
Tsp45I GTSAC 1 cut(s) 389
TspDTI ATGAA 1 cut(s) 411
TspGWI ACGGA 1 cut(s) 142
TspRI CASTG 2 cut(s) 150, 299
XspI CTAG 1 cut(s) 375
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.