pycom06g18650

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0016706
Physical Location & Seq
Forward (+)
22320067 .. 22321041
975 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g18650.1

Sequence Viewer

Length: 585 bp
ATGGAAACAACATCACCAACACTACCTGAACCAGCAGGAGATCAAATCTTGGGTGGTGGTACTGGTAGTAGCAGTAATATTAGTAATACTATTCCCCAAGCAGTACCATTAGTTTCTTCTTCTTCTTCGGGTTCCATTGGGCCATTCTTTGCAGTGATCTCTGTCCTAACCGTCCTTGCATTTCTCTCGTGCGTTTTGGGTCGGAGGCTGACTCGTGATGAAATGGTAACGACCCCGGTGGAGAGCATCAGGGATAGAATCGGCTGTTTTGGGTGGTTGAAGCGCAAGTGCCGGCAGCTGCAGTGCATGGTTGCTCATCATGACGTCGAAGTTGGATCGATCTTTGGCGATCAAAAGATGATGGTCATGGATCTTGGTCGAGAAAAGAAAGGTGATGCTAGGGTTAAAGAAGTGTTGAAAGTTACCACCAAGACACCTCAACTGAGCTGTACATGCGAATCATGGTTGACGGTGTTACTAACGACAAAAACTGGGGGTTTGACTTGCATGCTTGGATCCAGCCTTGCACATACCTCATCAGCTTCACTCCTTATCTTTTCCTTTAATTCAGCTGCTTTGATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

195

Amino Acids

20.66

Weight (kDa)

8.52

Isoelectric Point (pI)

47.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016706)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G74055
malus_domestica MD14G1219300.v1.1
prunus_persica Prupe.5G214000_v2.0.a1
pyrus_communis pycom06g18650 pycom14g18170
rosa_chinensis RchiOBHm_Chr7g0184281
rosa_laevigata RLG00000004990
rosa_multiflora Rmu_co8406705.1_g000001
rosa_roxburghii Rroxscaffold_3G00269770
rosa_samantha Rh7AG081500 Rh7BG065800 Rh7CG066100 Rh7DG065800
rosa_wichuraiana Rw7G005390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 327
AclWI GGATC 4 cut(s) 343, 378, 510, 523
AcyI GRCGYC 1 cut(s) 324
AfaI GTAC 3 cut(s) 61, 105, 451
AgsI TTSAA 2 cut(s) 280, 418
AluBI AGCT 4 cut(s) 298, 447, 542, 572
AluI AGCT 4 cut(s) 298, 447, 542, 572
AlwI GGATC 4 cut(s) 343, 378, 510, 523
AoxI GGCC 1 cut(s) 140
ApeKI GCWGC 3 cut(s) 295, 298, 572
AspLEI GCGC 1 cut(s) 285
AspS9I GGNCC 1 cut(s) 140
AsuC2I CCSGG 1 cut(s) 236
AsuHPI GGTGA 2 cut(s) 6, 404
BamHI GGATCC 1 cut(s) 515
BauI CACGAG 2 cut(s) 187, 213
BbvI GCAGC 3 cut(s) 285, 307, 559
BccI CCATC 1 cut(s) 355
BcgI CGANNNNNNTGC 2 cut(s) 168, 202
BcnI CCSGG 1 cut(s) 236
BfaI CTAG 1 cut(s) 399
BfmI CTRYAG 1 cut(s) 299
BisI GCNGC 3 cut(s) 296, 299, 573
BlsI GCNGC 3 cut(s) 297, 300, 574
Bme1390I CCNGG 1 cut(s) 236
BmgT120I GGNCC 1 cut(s) 140
BmiI GGNNCC 2 cut(s) 133, 517
BmrFI CCNGG 1 cut(s) 236
BmrI ACTGGG 1 cut(s) 501
BmsI GCATC 2 cut(s) 255, 385
BmuI ACTGGG 1 cut(s) 501
BplI GAGNNNNNCTC 2 cut(s) 196, 228
BpuMI CCSGG 1 cut(s) 236
Bsa29I ATCGAT 1 cut(s) 338
BsaHI GRCGYC 1 cut(s) 324
BsaJI CCNNGG 1 cut(s) 234
Bse118I RCCGGY 1 cut(s) 291
Bse1I ACTGG 2 cut(s) 67, 496
BseCI ATCGAT 1 cut(s) 338
BseDI CCNNGG 1 cut(s) 234
BseMII CTCAG 1 cut(s) 434
BseNI ACTGG 2 cut(s) 67, 496
BseXI GCAGC 3 cut(s) 285, 307, 559
BshFI GGCC 1 cut(s) 142
BshVI ATCGAT 1 cut(s) 338
BsiSI CCGG 2 cut(s) 236, 292
BsnI GGCC 1 cut(s) 142
Bsp1407I TGTACA 1 cut(s) 449
Bsp143I GATC 7 cut(s) 40, 156, 335, 339, 349, 370, 515
BspANI GGCC 1 cut(s) 142
BspCNI CTCAG 1 cut(s) 435
BspDI ATCGAT 1 cut(s) 338
BspHI TCATGA 1 cut(s) 319
BspLI GGNNCC 2 cut(s) 133, 517
BspMAI CTGCAG 1 cut(s) 303
BspPI GGATC 4 cut(s) 343, 378, 510, 523
BsrFI RCCGGY 1 cut(s) 291
BsrGI TGTACA 1 cut(s) 449
BsrI ACTGG 2 cut(s) 67, 496
BssAI RCCGGY 1 cut(s) 291
BssECI CCNNGG 1 cut(s) 234
BssMI GATC 7 cut(s) 40, 156, 335, 339, 349, 370, 515
BssNI GRCGYC 1 cut(s) 324
BssSI CACGAG 2 cut(s) 187, 213
Bst2BI CACGAG 2 cut(s) 187, 213
Bst4CI ACNGT 2 cut(s) 172, 472
BstACI GRCGYC 1 cut(s) 324
BstAUI TGTACA 1 cut(s) 449
BstC8I GCNNGC 2 cut(s) 293, 509
BstDEI CTNAG 1 cut(s) 443
BstHHI GCGC 1 cut(s) 285
BstKTI GATC 7 cut(s) 43, 159, 338, 342, 352, 373, 518
BstMBI GATC 7 cut(s) 40, 156, 335, 339, 349, 370, 515
BstMWI GCNNNNNNNGC 1 cut(s) 453
BstNSI RCATGY 2 cut(s) 456, 511
BstSCI CCNGG 1 cut(s) 234
BstSFI CTRYAG 1 cut(s) 299
BstV1I GCAGC 3 cut(s) 285, 307, 559
BstX2I RGATCY 2 cut(s) 370, 515
BstYI RGATCY 2 cut(s) 370, 515
Bsu15I ATCGAT 1 cut(s) 338
BsuRI GGCC 1 cut(s) 142
BsuTUI ATCGAT 1 cut(s) 338
BtsI GCAGTG 2 cut(s) 159, 308
BtsIMutI CAGTG 2 cut(s) 159, 308
Cac8I GCNNGC 2 cut(s) 293, 509
CciI TCATGA 1 cut(s) 319
CfoI GCGC 1 cut(s) 285
Cfr10I RCCGGY 1 cut(s) 291
Cfr13I GGNCC 1 cut(s) 140
ClaI ATCGAT 1 cut(s) 338
Csp6I GTAC 3 cut(s) 60, 104, 450
CviAII CATG 6 cut(s) 307, 320, 367, 453, 462, 508
CviJI RGCY 8 cut(s) 142, 208, 264, 298, 447, 522, 542, 572
CviKI_1 RGCY 8 cut(s) 142, 208, 264, 298, 447, 522, 542, 572
CviQI GTAC 3 cut(s) 60, 104, 450
DdeI CTNAG 1 cut(s) 443
DpnI GATC 7 cut(s) 42, 158, 337, 341, 351, 372, 517
DpnII GATC 7 cut(s) 40, 156, 335, 339, 349, 370, 515
FaeI CATG 6 cut(s) 310, 323, 370, 456, 465, 511
FaiI YATR 7 cut(s) 308, 321, 368, 454, 463, 509, 531
FatI CATG 6 cut(s) 306, 319, 366, 452, 461, 507
Fnu4HI GCNGC 3 cut(s) 296, 299, 573
Fsp4HI GCNGC 3 cut(s) 296, 299, 573
FspBI CTAG 1 cut(s) 399
GlaI GCGC 1 cut(s) 284
GluI GCNGC 3 cut(s) 296, 299, 573
HaeIII GGCC 1 cut(s) 142
HapII CCGG 2 cut(s) 236, 292
HhaI GCGC 1 cut(s) 285
Hin1I GRCGYC 1 cut(s) 324
Hin1II CATG 6 cut(s) 310, 323, 370, 456, 465, 511
Hin6I GCGC 1 cut(s) 283
HinP1I GCGC 1 cut(s) 283
HincII GTYRAC 1 cut(s) 468
HindII GTYRAC 1 cut(s) 468
HinfI GANTC 3 cut(s) 211, 258, 458
HpaII CCGG 2 cut(s) 236, 292
HphI GGTGA 2 cut(s) 6, 404
Hpy166II GTNNAC 1 cut(s) 468
Hpy188I TCNGA 1 cut(s) 204
Hpy188III TCNNGA 3 cut(s) 215, 320, 380
Hpy8I GTNNAC 1 cut(s) 468
Hpy99I CGWCG 1 cut(s) 329
HpyCH4III ACNGT 2 cut(s) 172, 472
HpyCH4IV ACGT 1 cut(s) 324
HpyCH4V TGCA 6 cut(s) 152, 179, 301, 306, 507, 527
HpyF10VI GCNNNNNNNGC 1 cut(s) 453
HpyF3I CTNAG 1 cut(s) 443
HpySE526I ACGT 1 cut(s) 324
Hsp92I GRCGYC 1 cut(s) 324
Hsp92II CATG 6 cut(s) 310, 323, 370, 456, 465, 511
HspAI GCGC 1 cut(s) 283
KroI GCCGGC 1 cut(s) 291
KroNI GCCGGC 1 cut(s) 293
Kzo9I GATC 7 cut(s) 40, 156, 335, 339, 349, 370, 515
LpnPI CCDG 9 cut(s) 21, 39, 45, 48, 235, 249, 305, 477, 532
Lsp1109I GCAGC 3 cut(s) 285, 307, 559
LweI GCATC 2 cut(s) 255, 385
MaeI CTAG 1 cut(s) 399
MaeII ACGT 1 cut(s) 324
MaeIII GTNAC 3 cut(s) 226, 421, 474
MalI GATC 7 cut(s) 42, 158, 337, 341, 351, 372, 517
MboI GATC 7 cut(s) 40, 156, 335, 339, 349, 370, 515
MboII GAAGA 4 cut(s) 108, 111, 114, 117
MflI RGATCY 2 cut(s) 370, 515
MluCI AATT 1 cut(s) 565
MlyI GAGTC 1 cut(s) 205
MmeI TCCRAC 2 cut(s) 182, 313
MnlI CCTC 3 cut(s) 198, 447, 544
MroNI GCCGGC 1 cut(s) 291
MseI TTAA 3 cut(s) 405, 564, 583
MspA1I CMGCKG 2 cut(s) 298, 572
MspI CCGG 2 cut(s) 236, 292
MspR9I CCNGG 1 cut(s) 236
MwoI GCNNNNNNNGC 1 cut(s) 453
NaeI GCCGGC 1 cut(s) 293
NciI CCSGG 1 cut(s) 236
NdeII GATC 7 cut(s) 40, 156, 335, 339, 349, 370, 515
NgoMIV GCCGGC 1 cut(s) 291
NlaIII CATG 6 cut(s) 310, 323, 370, 456, 465, 511
NlaIV GGNNCC 2 cut(s) 133, 517
NspI RCATGY 2 cut(s) 456, 511
PaeI GCATGC 1 cut(s) 511
PagI TCATGA 1 cut(s) 319
PdiI GCCGGC 1 cut(s) 293
PfeI GAWTC 2 cut(s) 258, 458
PkrI GCNGC 3 cut(s) 297, 300, 574
PleI GAGTC 1 cut(s) 205
PpsI GAGTC 1 cut(s) 205
PspN4I GGNNCC 2 cut(s) 133, 517
PspPI GGNCC 1 cut(s) 140
PstI CTGCAG 1 cut(s) 303
PsuI RGATCY 2 cut(s) 370, 515
PvuII CAGCTG 2 cut(s) 298, 572
RsaI GTAC 3 cut(s) 61, 105, 451
RsaNI GTAC 3 cut(s) 60, 104, 450
SaqAI TTAA 3 cut(s) 405, 564, 583
SatI GCNGC 3 cut(s) 296, 299, 573
Sau3AI GATC 7 cut(s) 40, 156, 335, 339, 349, 370, 515
Sau96I GGNCC 1 cut(s) 140
SchI GAGTC 1 cut(s) 205
ScrFI CCNGG 1 cut(s) 236
SetI ASST 9 cut(s) 28, 300, 327, 394, 439, 449, 536, 544, 574
SfaNI GCATC 2 cut(s) 255, 385
SfcI CTRYAG 1 cut(s) 299
SphI GCATGC 1 cut(s) 511
Sse9I AATT 1 cut(s) 565
SspI AATATT 1 cut(s) 79
SspMI CTAG 1 cut(s) 399
StyD4I CCNGG 1 cut(s) 234
TaaI ACNGT 2 cut(s) 172, 472
TaiI ACGT 1 cut(s) 327
TaqI TCGA 3 cut(s) 327, 338, 379
TasI AATT 1 cut(s) 565
TatI WGTACW 1 cut(s) 449
TfiI GAWTC 2 cut(s) 258, 458
Tru1I TTAA 3 cut(s) 405, 564, 583
Tru9I TTAA 3 cut(s) 405, 564, 583
TscAI CASTG 2 cut(s) 159, 308
TseI GCWGC 3 cut(s) 295, 298, 572
TspDTI ATGAA 1 cut(s) 234
TspRI CASTG 2 cut(s) 159, 308
XceI RCATGY 2 cut(s) 456, 511
XspI CTAG 1 cut(s) 399
ZraI GACGTC 1 cut(s) 325
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.