MD14G1223500.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0014089
Physical Location & Seq
Reverse (-)
30456903 .. 30457649
747 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1223500.v1.1.491

Sequence Viewer

Length: 747 bp
ATGGTGGTGATTCATGGCGTTGTGCCTAATCTATACGTGTATACCTCTTTGATCAAAGGGCTTTGTAAGATTGGTGAAATGAATTTGGCTTTTAGGCTGAAAGAGGAGATGGCAACGAAGAAAATCAAACCCGATTCAGCTGTTAATTCAACTTTAATCAGCGGGCTTTACAATGCTGGCCGGAAAGAAGAGGTTTCCGGGCTTTTGGACGAAATGAGATTGTACGGGTGCAAACCAGATACTGTGACTTTTAATGCATTGATTCACGGGCTGTGTAAAGAGAAGAATTTTGAAGCAGCATACAGAGTTTTGGATGAGATGGCGGAGAAAGGGTGTGAGCCAGATGTTATTAGTTACAATACAATTATCGGTGGACTATGCAAGGAAGGGAAATGGAGGGAAGCTAATGATTTGCTCGAAGATTTACCAAGACGCGGGTGTACTCCCGACTTTGTTTCATATCGAACAATGTTCGATGGACTTTGCGATTGGAGACAGTTCAAAGAAGCAGCCCTTGTTTTGGACGAGATGGTCTTCAAGGGCTTCGCACCCCGGCTTGCAAGTACACAGAAACTTGTTGAGGTGTTGTGTGAGGAAGGTAGTGCAGAGTTGTTAGGGACAGTTTTAACTAGTCTGGGAAAGGGAAAGGGCAAATTTCTTCGTGTAGATATGTGGGGAATGCTGACTGATATGGTTTGCAAGAAGGACAAGCTTGCAAATGTATTTAAACTTGTTGATATAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_1 PPR_2 PPR_3

Protein Analysis

249

Amino Acids

27.71

Weight (kDa)

6.22

Isoelectric Point (pI)

8.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_1 PF12854 6 - 37 3.3e-10 PPR repeat
PPR_2 PF13041 9 - 57 1.8e-11 PPR repeat family
PPR_3 PF13812 32 - 89 2.3e-08 Pentatricopeptide repeat domain
PPR_2 PF13041 51 - 93 1.2e-11 PPR repeat family
PPR_3 PF13812 68 - 124 4.5e-15 Pentatricopeptide repeat domain
PPR_1 PF12854 76 - 107 1.9e-13 PPR repeat
PPR_2 PF13041 79 - 128 9.5e-21 PPR repeat family
PPR PF01535 82 - 112 1.7e-08 PPR repeat
PPR_1 PF12854 110 - 141 3.4e-12 PPR repeat
PPR_2 PF13041 114 - 163 1.1e-18 PPR repeat family
PPR PF01535 117 - 147 9.8e-08 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014089)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G53330 AT1G53330
fragaria_vesca FvH4_5g12530
malus_domestica MD14G1223500.v1.1
prunus_persica Prupe.5G218700_v2.0.a1
pyrus_communis pycom06g18980 pycom14g18480
rosa_chinensis RchiOBHm_Chr7g0184831
rosa_laevigata RLG00000004951
rosa_multiflora Rmu_ssc0000289.1_g000014
rosa_roxburghii Rroxscaffold_3G00269350
rosa_rugosa Rorug06G0465200
rosa_samantha Rh7AG077700 Rh7BG069600 Rh7CG070400 Rh7DG069800
rosa_wichuraiana Rw7G005770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 530
AccI GTMKAC 1 cut(s) 41
AccII CGCG 1 cut(s) 435
AciI CCGC 3 cut(s) 162, 323, 435
AcoI YGGCCR 1 cut(s) 178
AcsI RAATTY 3 cut(s) 82, 286, 653
AfaI GTAC 3 cut(s) 224, 442, 565
AfiI CCNNNNNNNGG 2 cut(s) 434, 520
AflIII ACRYGT 1 cut(s) 36
AgsI TTSAA 4 cut(s) 150, 293, 502, 538
AhlI ACTAGT 1 cut(s) 629
AluBI AGCT 3 cut(s) 140, 404, 712
AluI AGCT 3 cut(s) 140, 404, 712
Alw26I GTCTC 1 cut(s) 487
AlwNI CAGNNNCTG 1 cut(s) 242
AoxI GGCC 1 cut(s) 178
ApeKI GCWGC 2 cut(s) 296, 509
ApoI RAATTY 3 cut(s) 82, 286, 653
AsuC2I CCSGG 2 cut(s) 199, 553
AsuHPI GGTGA 2 cut(s) 19, 86
BbsI GAAGAC 1 cut(s) 526
BbvI GCAGC 2 cut(s) 308, 521
BccI CCATC 4 cut(s) 103, 313, 470, 523
BclI TGATCA 1 cut(s) 51
BcnI CCSGG 2 cut(s) 199, 553
BcoDI GTCTC 1 cut(s) 487
BcuI ACTAGT 1 cut(s) 629
BfaI CTAG 1 cut(s) 630
BisI GCNGC 2 cut(s) 297, 510
BlsI GCNGC 2 cut(s) 298, 511
Bme1390I CCNGG 2 cut(s) 199, 553
BmrFI CCNGG 2 cut(s) 199, 553
BpiI GAAGAC 1 cut(s) 526
BpuMI CCSGG 2 cut(s) 199, 553
BsaAI YACGTR 1 cut(s) 37
BsaJI CCNNGG 1 cut(s) 551
BsaXI ACNNNNNCTCC 2 cut(s) 317, 347
Bsc4I CCNNNNNNNGG 2 cut(s) 434, 520
BseDI CCNNGG 1 cut(s) 551
BseGI GGATG 1 cut(s) 319
BseLI CCNNNNNNNGG 2 cut(s) 434, 520
BseRI GAGGAG 1 cut(s) 119
BseXI GCAGC 2 cut(s) 308, 521
BsgI GTGCAG 1 cut(s) 624
Bsh1236I CGCG 1 cut(s) 435
BshFI GGCC 1 cut(s) 180
BsiSI CCGG 3 cut(s) 181, 198, 553
BslFI GGGAC 1 cut(s) 631
BslI CCNNNNNNNGG 2 cut(s) 434, 520
BsmAI GTCTC 1 cut(s) 487
BsmFI GGGAC 1 cut(s) 631
BsmI GAATGC 1 cut(s) 684
BsnI GGCC 1 cut(s) 180
Bsp143I GATC 1 cut(s) 51
BspACI CCGC 3 cut(s) 162, 323, 435
BspANI GGCC 1 cut(s) 180
BspFNI CGCG 1 cut(s) 435
BssECI CCNNGG 1 cut(s) 551
BssMI GATC 1 cut(s) 51
BssNAI GTATAC 1 cut(s) 42
Bst1107I GTATAC 1 cut(s) 42
Bst4CI ACNGT 3 cut(s) 244, 498, 622
Bst6I CTCTTC 1 cut(s) 183
BstBAI YACGTR 1 cut(s) 37
BstC8I GCNNGC 4 cut(s) 164, 178, 558, 714
BstF5I GGATG 1 cut(s) 319
BstFNI CGCG 1 cut(s) 435
BstKTI GATC 1 cut(s) 54
BstMAI GTCTC 1 cut(s) 487
BstMBI GATC 1 cut(s) 51
BstSCI CCNGG 2 cut(s) 197, 551
BstUI CGCG 1 cut(s) 435
BstV1I GCAGC 2 cut(s) 308, 521
BstV2I GAAGAC 1 cut(s) 526
BstZ17I GTATAC 1 cut(s) 42
BsuRI GGCC 1 cut(s) 180
BtsCI GGATG 1 cut(s) 319
Cac8I GCNNGC 4 cut(s) 164, 178, 558, 714
CaiI CAGNNNCTG 1 cut(s) 242
CseI GACGC 1 cut(s) 441
Csp6I GTAC 3 cut(s) 223, 441, 564
CviAII CATG 1 cut(s) 14
CviQI GTAC 3 cut(s) 223, 441, 564
DpnI GATC 1 cut(s) 53
DpnII GATC 1 cut(s) 51
DraI TTTAAA 1 cut(s) 727
DrdI GACNNNNNNGTC 1 cut(s) 530
DseDI GACNNNNNNGTC 1 cut(s) 530
EaeI YGGCCR 1 cut(s) 178
Eam1104I CTCTTC 1 cut(s) 183
EarI CTCTTC 1 cut(s) 183
EciI GGCGGA 1 cut(s) 338
EcoT22I ATGCAT 1 cut(s) 259
FaeI CATG 1 cut(s) 17
FaiI YATR 9 cut(s) 15, 34, 42, 301, 379, 460, 671, 692, 740
FalI AAGNNNNNCTT 2 cut(s) 498, 530
FaqI GGGAC 1 cut(s) 631
FatI CATG 1 cut(s) 13
FauI CCCGC 2 cut(s) 155, 428
FbaI TGATCA 1 cut(s) 51
FblI GTMKAC 1 cut(s) 41
Fnu4HI GCNGC 2 cut(s) 297, 510
FokI GGATG 1 cut(s) 326
Fsp4HI GCNGC 2 cut(s) 297, 510
FspBI CTAG 1 cut(s) 630
GluI GCNGC 2 cut(s) 297, 510
HaeIII GGCC 1 cut(s) 180
HapII CCGG 3 cut(s) 181, 198, 553
HgaI GACGC 1 cut(s) 441
Hin1II CATG 1 cut(s) 17
HindIII AAGCTT 1 cut(s) 710
HinfI GANTC 3 cut(s) 10, 134, 262
HpaII CCGG 3 cut(s) 181, 198, 553
HphI GGTGA 2 cut(s) 19, 86
Hpy166II GTNNAC 4 cut(s) 42, 374, 441, 566
Hpy188III TCNNGA 1 cut(s) 446
Hpy8I GTNNAC 4 cut(s) 42, 374, 441, 566
HpyAV CCTTC 3 cut(s) 380, 590, 697
HpyCH4III ACNGT 3 cut(s) 244, 498, 622
HpyCH4IV ACGT 1 cut(s) 36
HpyCH4V TGCA 7 cut(s) 231, 257, 381, 560, 605, 699, 716
HpySE526I ACGT 1 cut(s) 36
Hsp92II CATG 1 cut(s) 17
Ksp22I TGATCA 1 cut(s) 51
Kzo9I GATC 1 cut(s) 51
LpnPI CCDG 7 cut(s) 162, 194, 211, 249, 354, 566, 620
Lsp1109I GCAGC 2 cut(s) 308, 521
MaeI CTAG 1 cut(s) 630
MaeII ACGT 1 cut(s) 36
MaeIII GTNAC 2 cut(s) 244, 353
MalI GATC 1 cut(s) 53
MboI GATC 1 cut(s) 51
MboII GAAGA 6 cut(s) 130, 200, 295, 431, 526, 650
MluCI AATT 5 cut(s) 82, 145, 286, 363, 653
MnlI CCTC 6 cut(s) 55, 97, 184, 390, 574, 586
Mph1103I ATGCAT 1 cut(s) 259
MseI TTAA 5 cut(s) 144, 155, 252, 626, 726
MspA1I CMGCKG 2 cut(s) 140, 162
MspI CCGG 3 cut(s) 181, 198, 553
MspR9I CCNGG 2 cut(s) 199, 553
Mva1269I GAATGC 1 cut(s) 684
MvnI CGCG 1 cut(s) 435
NciI CCSGG 2 cut(s) 199, 553
NdeII GATC 1 cut(s) 51
NlaIII CATG 1 cut(s) 17
NmuCI GTSAC 1 cut(s) 244
NsiI ATGCAT 1 cut(s) 259
PctI GAATGC 1 cut(s) 684
PfeI GAWTC 3 cut(s) 10, 134, 262
PkrI GCNGC 2 cut(s) 298, 511
Ppu21I YACGTR 1 cut(s) 37
PstNI CAGNNNCTG 1 cut(s) 242
PvuII CAGCTG 1 cut(s) 140
RsaI GTAC 3 cut(s) 224, 442, 565
RsaNI GTAC 3 cut(s) 223, 441, 564
SaqAI TTAA 5 cut(s) 144, 155, 252, 626, 726
SatI GCNGC 2 cut(s) 297, 510
Sau3AI GATC 1 cut(s) 51
ScrFI CCNGG 2 cut(s) 199, 553
SetI ASST 8 cut(s) 39, 47, 142, 195, 406, 585, 601, 714
SpeI ACTAGT 1 cut(s) 629
Sse9I AATT 5 cut(s) 82, 145, 286, 363, 653
SsiI CCGC 3 cut(s) 162, 323, 435
SspMI CTAG 1 cut(s) 630
StyD4I CCNGG 2 cut(s) 197, 551
TaaI ACNGT 3 cut(s) 244, 498, 622
TaiI ACGT 1 cut(s) 39
TaqI TCGA 3 cut(s) 417, 463, 474
TasI AATT 5 cut(s) 82, 145, 286, 363, 653
TatI WGTACW 2 cut(s) 440, 563
TfiI GAWTC 3 cut(s) 10, 134, 262
Tru1I TTAA 5 cut(s) 144, 155, 252, 626, 726
Tru9I TTAA 5 cut(s) 144, 155, 252, 626, 726
TseFI GTSAC 1 cut(s) 244
TseI GCWGC 2 cut(s) 296, 509
Tsp45I GTSAC 1 cut(s) 244
TspDTI ATGAA 2 cut(s) 95, 447
XapI RAATTY 3 cut(s) 82, 286, 653
XmiI GTMKAC 1 cut(s) 41
XspI CTAG 1 cut(s) 630
Zsp2I ATGCAT 1 cut(s) 259
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.