MD15G1013100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0017927
Physical Location & Seq
Reverse (-)
718535 .. 718816
282 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1013100.v1.1.491

Sequence Viewer

Length: 282 bp
ATGAAAATCTTCAATCGCTTCAGGAAGATAATAATGAAACTTATTTTTACTGTCCCGTCTCGATCGGCCCGCGGCACATCTCCGTCGGGAGCTACGAGCACTCCCAGGCAAAGGAACAGCAGCTGTGACAGATTTGAGCCTCCGAAGACTTCATGCAGTTCGTACTACTCGTCGCACTTGCATTATAACGAGGCCATCGCCGACTGCATCGAGTTCTTTAATAAGTCTTCTTCTGGCCAAGAGGGCAGTTTGGATGATGGAGAATTCAACGTTATGGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

94

Amino Acids

10.45

Weight (kDa)

8.93

Isoelectric Point (pI)

61.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017927)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G75717
malus_domestica MD15G1013100.v1.1
prunus_persica Prupe.1G366200_v2.0.a1
rosa_laevigata RLG00000011140
rosa_roxburghii Rroxscaffold_1G00056990 Rroxscaffold_7G00165350
rosa_rugosa Rorug06G0316700
rosa_samantha Rh6AG430100 Rh6CG442600 Rh6DG429300
rosa_wichuraiana Rw6G037250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 186
AccII CGCG 1 cut(s) 72
AciI CCGC 2 cut(s) 70, 72
AclI AACGTT 1 cut(s) 270
AcoI YGGCCR 1 cut(s) 235
AcsI RAATTY 1 cut(s) 263
AcuI CTGAAG 1 cut(s) 4
AfaI GTAC 1 cut(s) 164
AfiI CCNNNNNNNGG 1 cut(s) 111
AgsI TTSAA 2 cut(s) 13, 268
AjnI CCWGG 1 cut(s) 104
AluBI AGCT 2 cut(s) 92, 123
AluI AGCT 2 cut(s) 92, 123
Alw21I GWGCWC 1 cut(s) 101
Alw26I GTCTC 1 cut(s) 63
AlwNI CAGNNNCTG 1 cut(s) 123
AoxI GGCC 3 cut(s) 66, 192, 235
ApeKI GCWGC 1 cut(s) 120
ApoI RAATTY 1 cut(s) 263
Asp700I GAANNNNTTC 1 cut(s) 8
AspS9I GGNCC 1 cut(s) 67
BalI TGGCCA 1 cut(s) 237
BbsI GAAGAC 2 cut(s) 152, 219
Bbv12I GWGCWC 1 cut(s) 101
BbvI GCAGC 1 cut(s) 132
BccI CCATC 2 cut(s) 203, 251
BciT130I CCWGG 1 cut(s) 106
BcoDI GTCTC 1 cut(s) 63
BglI GCCNNNNNGGC 1 cut(s) 243
BisI GCNGC 2 cut(s) 73, 121
BlsI GCNGC 2 cut(s) 74, 122
Bme1390I CCNGG 1 cut(s) 106
BmgT120I GGNCC 1 cut(s) 67
BmrFI CCNGG 1 cut(s) 106
BmsI GCATC 1 cut(s) 216
BpiI GAAGAC 2 cut(s) 152, 219
BsaJI CCNNGG 2 cut(s) 70, 104
BsaXI ACNNNNNCTCC 2 cut(s) 85, 115
Bsc4I CCNNNNNNNGG 1 cut(s) 111
BseBI CCWGG 1 cut(s) 106
BseDI CCNNGG 2 cut(s) 70, 104
BseGI GGATG 1 cut(s) 259
BseLI CCNNNNNNNGG 1 cut(s) 111
BseXI GCAGC 1 cut(s) 132
Bsh1236I CGCG 1 cut(s) 72
Bsh1285I CGRYCG 1 cut(s) 65
BshFI GGCC 3 cut(s) 68, 194, 237
BsiEI CGRYCG 1 cut(s) 65
BsiHKAI GWGCWC 1 cut(s) 101
BslFI GGGAC 1 cut(s) 38
BslI CCNNNNNNNGG 1 cut(s) 111
BsmAI GTCTC 1 cut(s) 63
BsmBI CGTCTC 1 cut(s) 63
BsmFI GGGAC 1 cut(s) 38
BsnI GGCC 3 cut(s) 68, 194, 237
Bsp1286I GDGCHC 1 cut(s) 101
Bsp143I GATC 1 cut(s) 62
BspACI CCGC 2 cut(s) 70, 72
BspANI GGCC 3 cut(s) 68, 194, 237
BspFNI CGCG 1 cut(s) 72
BssECI CCNNGG 2 cut(s) 70, 104
BssMI GATC 1 cut(s) 62
Bst2UI CCWGG 1 cut(s) 106
Bst4CI ACNGT 1 cut(s) 52
BstC8I GCNNGC 1 cut(s) 70
BstDSI CCRYGG 1 cut(s) 70
BstF5I GGATG 1 cut(s) 259
BstFNI CGCG 1 cut(s) 72
BstKTI GATC 1 cut(s) 65
BstMAI GTCTC 1 cut(s) 63
BstMBI GATC 1 cut(s) 62
BstMCI CGRYCG 1 cut(s) 65
BstMWI GCNNNNNNNGC 1 cut(s) 243
BstNI CCWGG 1 cut(s) 106
BstSCI CCNGG 1 cut(s) 104
BstUI CGCG 1 cut(s) 72
BstV1I GCAGC 1 cut(s) 132
BstV2I GAAGAC 2 cut(s) 152, 219
BsuRI GGCC 3 cut(s) 68, 194, 237
BtgI CCRYGG 1 cut(s) 70
BtgZI GCGATG 1 cut(s) 181
BtsCI GGATG 1 cut(s) 259
Cac8I GCNNGC 1 cut(s) 70
CaiI CAGNNNCTG 1 cut(s) 123
Cfr13I GGNCC 1 cut(s) 67
Cfr42I CCGCGG 1 cut(s) 73
Csp6I GTAC 1 cut(s) 163
CviAII CATG 1 cut(s) 153
CviJI RGCY 6 cut(s) 68, 92, 123, 139, 194, 237
CviKI_1 RGCY 6 cut(s) 68, 92, 123, 139, 194, 237
CviQI GTAC 1 cut(s) 163
DpnI GATC 1 cut(s) 64
DpnII GATC 1 cut(s) 62
EaeI YGGCCR 1 cut(s) 235
Eco57I CTGAAG 1 cut(s) 4
EcoRI GAATTC 1 cut(s) 263
EcoRII CCWGG 1 cut(s) 104
Esp3I CGTCTC 1 cut(s) 63
FaeI CATG 1 cut(s) 156
FaiI YATR 3 cut(s) 154, 186, 275
FaqI GGGAC 1 cut(s) 38
FatI CATG 1 cut(s) 152
FauI CCCGC 1 cut(s) 77
Fnu4HI GCNGC 2 cut(s) 73, 121
FokI GGATG 1 cut(s) 266
Fsp4HI GCNGC 2 cut(s) 73, 121
GluI GCNGC 2 cut(s) 73, 121
HaeIII GGCC 3 cut(s) 68, 194, 237
Hin1II CATG 1 cut(s) 156
Hpy188I TCNGA 2 cut(s) 144, 281
Hpy188III TCNNGA 3 cut(s) 22, 60, 87
Hpy99I CGWCG 2 cut(s) 88, 175
HpyCH4III ACNGT 1 cut(s) 52
HpyCH4IV ACGT 1 cut(s) 270
HpyCH4V TGCA 3 cut(s) 156, 181, 207
HpyF10VI GCNNNNNNNGC 1 cut(s) 243
HpySE526I ACGT 1 cut(s) 270
Hsp92II CATG 1 cut(s) 156
KspI CCGCGG 1 cut(s) 73
Kzo9I GATC 1 cut(s) 62
LmnI GCTCC 1 cut(s) 89
LpnPI CCDG 4 cut(s) 7, 91, 118, 219
Lsp1109I GCAGC 1 cut(s) 132
LweI GCATC 1 cut(s) 216
MaeII ACGT 1 cut(s) 270
MaeIII GTNAC 1 cut(s) 125
MalI GATC 1 cut(s) 64
MboI GATC 1 cut(s) 62
MboII GAAGA 4 cut(s) 37, 157, 219, 222
MhlI GDGCHC 1 cut(s) 101
MlsI TGGCCA 1 cut(s) 237
MluCI AATT 1 cut(s) 263
MluNI TGGCCA 1 cut(s) 237
MnlI CCTC 3 cut(s) 150, 184, 235
Mox20I TGGCCA 1 cut(s) 237
MroXI GAANNNNTTC 1 cut(s) 8
MscI TGGCCA 1 cut(s) 237
MseI TTAA 1 cut(s) 219
Msp20I TGGCCA 1 cut(s) 237
MspA1I CMGCKG 2 cut(s) 72, 123
MspR9I CCNGG 1 cut(s) 106
MvaI CCWGG 1 cut(s) 106
MvnI CGCG 1 cut(s) 72
MwoI GCNNNNNNNGC 1 cut(s) 243
NdeII GATC 1 cut(s) 62
NlaIII CATG 1 cut(s) 156
NmuCI GTSAC 1 cut(s) 125
PcsI WCGNNNNNNNCGW 2 cut(s) 92, 167
PdmI GAANNNNTTC 1 cut(s) 8
PkrI GCNGC 2 cut(s) 74, 122
Ple19I CGATCG 1 cut(s) 65
PsiI TTATAA 1 cut(s) 186
Psp1406I AACGTT 1 cut(s) 270
Psp6I CCWGG 1 cut(s) 104
PspGI CCWGG 1 cut(s) 104
PspPI GGNCC 1 cut(s) 67
PstNI CAGNNNCTG 1 cut(s) 123
PvuI CGATCG 1 cut(s) 65
PvuII CAGCTG 1 cut(s) 123
RsaI GTAC 1 cut(s) 164
RsaNI GTAC 1 cut(s) 163
SacII CCGCGG 1 cut(s) 73
SaqAI TTAA 1 cut(s) 219
SatI GCNGC 2 cut(s) 73, 121
Sau3AI GATC 1 cut(s) 62
Sau96I GGNCC 1 cut(s) 67
ScrFI CCNGG 1 cut(s) 106
SduI GDGCHC 1 cut(s) 101
SetI ASST 3 cut(s) 94, 125, 273
SfaNI GCATC 1 cut(s) 216
Sfr303I CCGCGG 1 cut(s) 73
SgrBI CCGCGG 1 cut(s) 73
Sse9I AATT 1 cut(s) 263
SsiI CCGC 2 cut(s) 70, 72
StyD4I CCNGG 1 cut(s) 104
TaaI ACNGT 1 cut(s) 52
TaiI ACGT 1 cut(s) 273
TaqI TCGA 2 cut(s) 61, 210
TasI AATT 1 cut(s) 263
TauI GCSGC 1 cut(s) 75
Tru1I TTAA 1 cut(s) 219
Tru9I TTAA 1 cut(s) 219
TseFI GTSAC 1 cut(s) 125
TseI GCWGC 1 cut(s) 120
Tsp45I GTSAC 1 cut(s) 125
TspDTI ATGAA 3 cut(s) 17, 50, 141
TspGWI ACGGA 1 cut(s) 72
XapI RAATTY 1 cut(s) 263
XmnI GAANNNNTTC 1 cut(s) 8
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.