Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0013918

Physical Location & Seq

Genomic Coordinates Forward (+)

788551 .. 791957

Gene Structure 3407 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1014100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1461 bp

ATGTCTCTCACCATTAAACTCCACGACTGCCATCTCAATCTCCTCAACAACAACCACCTCGGCATCTCCAAACCCAAAACCAAAGTCCCAAACCTCAAGCTCAAATTCCGAACCAAACCCATCTACCACTCCGACCCCTTACTCTCCGTCGTGTACAACTCCACAATGCACGCCTCCTCCGCTCGCCCGCCTCAATCCGCTGTCATCAAGAGCTCCCTCATCGACCCCGACGGCGGGGCCCTGGTCGATCTCGTGGTGCCGGAGAGCCAGAGAGCTGCTAAGGCTCTGGAGGCGGAGGCGCTGCCCAAGGTGCGGCTGACAAAGATTGATCTCGAGTGGGTCCACGTCATCAGCGAGGGGTGGGCCAGCCCGCTGCGGGGATTTATGAGAGAAGACCAGTACTTGCAGAGCTTGCATTTCAATTCGCTGCGGATCGAAGACGGGTCGTTTGTTAACATGTCGCTGCCCATTGTGTTGGCGATTGACGACGAGACTAAGGAGAGAATCGGGTCCTCGAATAATGTCGGGCTGGTCGGCCCGGGTGGAGATTTGGTTGGTGTATTAAGCAGCATTGAGATTTACAAGCATAACAAAGAAGAAAGGATTGCTAGGACTTGGGGGTCAACAGCCCCGGGGTTGCCATACGTTGAGGAGGTGATCACTCCGGCTGGAAACTGGCTTATTGGCGGAGATTTGGAAGTACTAGACCGTGTCAAATATAATGACGGGCTTGATCACTACAGGCTCTCTCCTCGACAGCTCCGGAAAGAATTTGACAGGCGTCAAGCTGACGCAGTTTTTGCTTTTCAGTTGAGAAATCCCGTACATAATGGCCATGCTTTGTTGATGAACGACACACGCAGGCGACTCTTGGACATGGGATACAAGAATCCAATTTTGTTGCTTCATCCTTTGGGAGGTTTCACAAAGCCTGACGATGTGCCATTGGATGTTCGGATGGAGCAACATAGCAAGGTCCTAGAAGATGGAGTTCTTGACCCTGAAACTACTATTGTGGGTATATTCCCATCACCTATGCACTACGCAGGTCCAACTGAAGTACAGTGGCATGCCAAAGCTCGGATAAATGCAGGAGCAAATTTCTACATTGTGGGCCGTGATCCTGCTGGTATGGGACACCCCACAGAGAATAGGGATTTATATGACCCTGATCATGGGAAAAAGGTGTTAAGCATGGCTCCCGGGCTCGAAAAGCTCAATATTTTGCCTTTCAGGGTGGCAGCTTATGACACTGTGGAAAAGAAGATGGCATTTTTTGATCCTTCACGCGCTAAAGATTTCCTCTTCATCTCTGGAACCAAGATGCGTGCTTATGCAAAGAGCGGCGAAAACCCTCCTGATGGTTTTATGTGTCCCTCGGGATGGAAGGTTCTCGTCAACTATTACAAGAGCTTGCAAACAGAAGAGGCATCACAGCAACAAGCTGTGTTATCTACTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000103 GO:0003674 GO:0003824 GO:0004020 GO:0004779 GO:0004781 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006790 GO:0006793 GO:0006796 GO:0006950 GO:0007154 GO:0008150 GO:0008152 GO:0009267 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009970 GO:0009987 GO:0009991 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016779 GO:0031667 GO:0031668 GO:0031669 GO:0033554 GO:0042594 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0050896 GO:0051716 GO:0070566 GO:0071496

KEGG Pathways

Metabolic & Signaling

ko00230 ko00261 ko00450 ko00920 ko01100 ko01120 ko01130 map00230 map00261 map00450 map00920 map01100 map01120 map01130

Pfam Domains

Protein Families

ATP-sulfurylase PUA_2

Protein Analysis

487

Amino Acids

54.19

Weight (kDa)

7.01

Isoelectric Point (pI)

48.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PUA_2	PF14306	74 - 236	4.6e-43	PUA-like domain
ATP-sulfurylase	PF01747	246 - 467	7.4e-68	ATP-sulfurylase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013918)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G19920
fragaria_vesca	FvH4_2g38710
malus_domestica	MD08G1014800.v1.1 MD15G1014100.v1.1
prunus_persica	Prupe.1G367700_v2.0.a1
pyrus_communis	pycom08g01320 pycom15g01220
rosa_chinensis	RchiOBHm_Chr6g0302601
rosa_laevigata	RLG00000011158
rosa_multiflora	Rmu_sc0005455.1_g000010
rosa_roxburghii	Rroxscaffold_7G00165530
rosa_rugosa	Rorug06G0315200
rosa_samantha	Rh6AG428600 Rh6BG473900 Rh6CG440800 Rh6DG427600
rosa_wichuraiana	Rw6G037090

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	619
Acc36I	ACCTGC	1 cut(s)	1037
AccB1I	GGYRCC	1 cut(s)	256
AccBSI	CCGCTC	2 cut(s)	182, 1344
AccII	CGCG	1 cut(s)	1290
AccIII	TCCGGA	1 cut(s)	762
AclWI	GGATC	3 cut(s)	440, 1115, 1274
AcoI	YGGCCR	1 cut(s)	832
AcsI	RAATTY	3 cut(s)	104, 770, 1099
AcuI	CTGAAG	1 cut(s)	1077
AcyI	GRCGYC	1 cut(s)	781
AfaI	GTAC	5 cut(s)	155, 401, 702, 825, 1062
AflIII	ACRYGT	1 cut(s)	456
AgsI	TTSAA	1 cut(s)	421
AjiI	CACGTC	1 cut(s)	346
AjnI	CCWGG	1 cut(s)	240
AloI	GAACNNNNNNTCC	2 cut(s)	1374, 1406
Alw21I	GWGCWC	1 cut(s)	215
Alw26I	GTCTC	2 cut(s)	9, 485
AlwI	GGATC	3 cut(s)	440, 1115, 1274
Ama87I	CYCGRG	5 cut(s)	332, 538, 631, 1202, 1378
Aor13HI	TCCGGA	1 cut(s)	762
AoxI	GGCC	5 cut(s)	237, 363, 535, 832, 1114
ApaI	GGGCCC	1 cut(s)	241
ApeKI	GCWGC	7 cut(s)	275, 301, 373, 427, 463, 567, 1241
ApoI	RAATTY	3 cut(s)	104, 770, 1099
ArsI	GACNNNNNNTTYG	6 cut(s)	69, 101, 431, 463, 782, 814
AspLEI	GCGC	2 cut(s)	301, 1292
AspS9I	GGNCC	9 cut(s)	237, 238, 340, 363, 510, 536, 976, 1049, 1114
AsuC2I	CCSGG	6 cut(s)	539, 540, 632, 633, 1203, 1204
AsuHPI	GGTGA	2 cut(s)	667, 1023
AvaI	CYCGRG	5 cut(s)	332, 538, 631, 1202, 1378
AvaII	GGWCC	4 cut(s)	340, 510, 976, 1049
BaeGI	GKGCMC	1 cut(s)	241
BalI	TGGCCA	1 cut(s)	834
BanI	GGYRCC	1 cut(s)	256
BanII	GRGCYC	3 cut(s)	215, 241, 1209
BauI	CACGAG	1 cut(s)	251
BbsI	GAAGAC	2 cut(s)	399, 444
Bbv12I	GWGCWC	1 cut(s)	215
BbvI	GCAGC	7 cut(s)	262, 288, 360, 414, 450, 579, 1253
BccI	CCATC	8 cut(s)	39, 128, 952, 980, 1036, 1261, 1355, 1377
BceAI	ACGGC	2 cut(s)	247, 1101
BciT130I	CCWGG	1 cut(s)	242
BciVI	GTATCC	1 cut(s)	875
BclI	TGATCA	3 cut(s)	657, 733, 1171
BcnI	CCSGG	6 cut(s)	539, 540, 632, 633, 1203, 1204
BcoDI	GTCTC	2 cut(s)	9, 485
BfaI	CTAG	3 cut(s)	609, 704, 980
BfmI	CTRYAG	1 cut(s)	739
BfoI	RGCGCY	1 cut(s)	302
BfuAI	ACCTGC	1 cut(s)	1037
BfuI	GTATCC	1 cut(s)	875
BisI	GCNGC	9 cut(s)	276, 302, 314, 374, 428, 464, 568, 1242, 1345
BlsI	GCNGC	9 cut(s)	277, 303, 315, 375, 429, 465, 569, 1243, 1346
BmcAI	AGTACT	2 cut(s)	401, 702
Bme1390I	CCNGG	7 cut(s)	242, 539, 540, 632, 633, 1203, 1204
Bme18I	GGWCC	4 cut(s)	340, 510, 976, 1049
BmeT110I	CYCGRG	5 cut(s)	332, 538, 631, 1202, 1378
BmgBI	CACGTC	1 cut(s)	346
BmgT120I	GGNCC	9 cut(s)	237, 238, 340, 363, 510, 536, 976, 1049, 1114
BmiI	GGNNCC	7 cut(s)	238, 239, 258, 341, 511, 1200, 1318
BmrFI	CCNGG	7 cut(s)	242, 539, 540, 632, 633, 1203, 1204
BmsI	GCATC	3 cut(s)	72, 1314, 1439
BoxI	GACNNNNGTC	1 cut(s)	780
BpiI	GAAGAC	2 cut(s)	399, 444
BpmI	CTGGAG	1 cut(s)	308
Bpu10I	CCTNAGC	1 cut(s)	279
BpuEI	CTTGAG	1 cut(s)	80
BpuMI	CCSGG	6 cut(s)	539, 540, 632, 633, 1203, 1204
BsaHI	GRCGYC	1 cut(s)	781
BsaJI	CCNNGG	9 cut(s)	58, 240, 306, 538, 630, 631, 632, 1202, 1377
BsaWI	WCCGGW	1 cut(s)	762
BsaXI	ACNNNNNCTCC	4 cut(s)	161, 191, 537, 567
Bse1I	ACTGG	2 cut(s)	397, 680
BseAI	TCCGGA	1 cut(s)	762
BseBI	CCWGG	1 cut(s)	242
BseDI	CCNNGG	9 cut(s)	58, 240, 306, 538, 630, 631, 632, 1202, 1377
BseGI	GGATG	4 cut(s)	907, 955, 963, 1388
BseNI	ACTGG	2 cut(s)	397, 680
BseRI	GAGGAG	4 cut(s)	32, 166, 665, 741
BseSI	GKGCMC	1 cut(s)	241
BseXI	GCAGC	7 cut(s)	262, 288, 360, 414, 450, 579, 1253
Bsh1236I	CGCG	1 cut(s)	1290
BshFI	GGCC	5 cut(s)	239, 365, 537, 834, 1116
BshNI	GGYRCC	1 cut(s)	256
BsiHKAI	GWGCWC	1 cut(s)	215
BsiHKCI	CYCGRG	5 cut(s)	332, 538, 631, 1202, 1378
BsiSI	CCGG	6 cut(s)	260, 539, 632, 665, 763, 1203
BslFI	GGGAC	3 cut(s)	71, 1149, 1359
BsmAI	GTCTC	2 cut(s)	9, 485
BsmFI	GGGAC	3 cut(s)	71, 1149, 1359
BsnI	GGCC	5 cut(s)	239, 365, 537, 834, 1116
BsoBI	CYCGRG	5 cut(s)	332, 538, 631, 1202, 1378
Bsp120I	GGGCCC	1 cut(s)	237
Bsp1286I	GDGCHC	3 cut(s)	215, 241, 1209
Bsp13I	TCCGGA	1 cut(s)	762
Bsp1407I	TGTACA	1 cut(s)	153
Bsp143I	GATC	8 cut(s)	247, 328, 432, 657, 733, 1120, 1171, 1279
BspANI	GGCC	5 cut(s)	239, 365, 537, 834, 1116
BspEI	TCCGGA	1 cut(s)	762
BspFNI	CGCG	1 cut(s)	1290
BspLI	GGNNCC	7 cut(s)	238, 239, 258, 341, 511, 1200, 1318
BspMI	ACCTGC	1 cut(s)	1037
BspPI	GGATC	3 cut(s)	440, 1115, 1274
BspT107I	GGYRCC	1 cut(s)	256
BsrBI	CCGCTC	2 cut(s)	182, 1344
BsrGI	TGTACA	1 cut(s)	153
BsrI	ACTGG	2 cut(s)	397, 680
BssECI	CCNNGG	9 cut(s)	58, 240, 306, 538, 630, 631, 632, 1202, 1377
BssMI	GATC	8 cut(s)	247, 328, 432, 657, 733, 1120, 1171, 1279
BssNI	GRCGYC	1 cut(s)	781
BssSI	CACGAG	1 cut(s)	251
BssT1I	CCWWGG	1 cut(s)	306
Bst2BI	CACGAG	1 cut(s)	251
Bst2UI	CCWGG	1 cut(s)	242
Bst4CI	ACNGT	3 cut(s)	710, 1065, 1255
Bst6I	CTCTTC	2 cut(s)	1310, 1419
BstACI	GRCGYC	1 cut(s)	781
BstAPI	GCANNNNNTGC	2 cut(s)	412, 800
BstAUI	TGTACA	1 cut(s)	153
BstDEI	CTNAG	3 cut(s)	279, 495, 1458
BstENI	CCTNNNNNAGG	1 cut(s)	915
BstF5I	GGATG	4 cut(s)	907, 955, 963, 1388
BstFNI	CGCG	1 cut(s)	1290
BstH2I	RGCGCY	1 cut(s)	302
BstHHI	GCGC	2 cut(s)	301, 1292
BstKTI	GATC	8 cut(s)	250, 331, 435, 660, 736, 1123, 1174, 1282
BstMAI	GTCTC	2 cut(s)	9, 485
BstMBI	GATC	8 cut(s)	247, 328, 432, 657, 733, 1120, 1171, 1279
BstMWI	GCNNNNNNNGC	7 cut(s)	179, 281, 290, 310, 412, 800, 1213
BstNI	CCWGG	1 cut(s)	242
BstNSI	RCATGY	2 cut(s)	460, 1073
BstPAI	GACNNNNGTC	1 cut(s)	780
BstSCI	CCNGG	7 cut(s)	240, 537, 538, 630, 631, 1201, 1202
BstSFI	CTRYAG	1 cut(s)	739
BstSLI	GKGCMC	1 cut(s)	241
BstUI	CGCG	1 cut(s)	1290
BstV1I	GCAGC	7 cut(s)	262, 288, 360, 414, 450, 579, 1253
BstV2I	GAAGAC	2 cut(s)	399, 444
BstXI	CCANNNNNNTGG	1 cut(s)	475
BsuI	GTATCC	1 cut(s)	875
BsuRI	GGCC	5 cut(s)	239, 365, 537, 834, 1116
BtrI	CACGTC	1 cut(s)	346
BtsCI	GGATG	4 cut(s)	907, 955, 963, 1388
BtsIMutI	CAGTG	2 cut(s)	1070, 1251
BveI	ACCTGC	1 cut(s)	1037
CfoI	GCGC	2 cut(s)	301, 1292
Cfr13I	GGNCC	9 cut(s)	237, 238, 340, 363, 510, 536, 976, 1049, 1114
Cfr9I	CCCGGG	3 cut(s)	538, 631, 1202
CseI	GACGC	2 cut(s)	770, 800
Csp6I	GTAC	5 cut(s)	154, 400, 701, 824, 1061
CviAII	CATG	6 cut(s)	457, 836, 877, 1070, 1175, 1195
CviQI	GTAC	5 cut(s)	154, 400, 701, 824, 1061
DdeI	CTNAG	3 cut(s)	279, 495, 1458
DpnI	GATC	8 cut(s)	249, 330, 434, 659, 735, 1122, 1173, 1281
DpnII	GATC	8 cut(s)	247, 328, 432, 657, 733, 1120, 1171, 1279
DrdI	GACNNNNNNGTC	1 cut(s)	619
DseDI	GACNNNNNNGTC	1 cut(s)	619
EaeI	YGGCCR	1 cut(s)	832
Eam1104I	CTCTTC	2 cut(s)	1310, 1419
EarI	CTCTTC	2 cut(s)	1310, 1419
EciI	GGCGGA	2 cut(s)	308, 702
Ecl136II	GAGCTC	1 cut(s)	213
Eco130I	CCWWGG	1 cut(s)	306
Eco24I	GRGCYC	3 cut(s)	215, 241, 1209
Eco47I	GGWCC	4 cut(s)	340, 510, 976, 1049
Eco53kI	GAGCTC	1 cut(s)	213
Eco57I	CTGAAG	1 cut(s)	1077
Eco88I	CYCGRG	5 cut(s)	332, 538, 631, 1202, 1378
EcoICRI	GAGCTC	1 cut(s)	213
EcoNI	CCTNNNNNAGG	1 cut(s)	915
EcoO109I	RGGNCCY	4 cut(s)	237, 238, 510, 976
EcoRII	CCWGG	1 cut(s)	240
EcoT14I	CCWWGG	1 cut(s)	306
EcoT38I	GRGCYC	3 cut(s)	215, 241, 1209
ErhI	CCWWGG	1 cut(s)	306
FaeI	CATG	6 cut(s)	460, 839, 880, 1073, 1178, 1198
FaqI	GGGAC	3 cut(s)	71, 1149, 1359
FatI	CATG	6 cut(s)	456, 835, 876, 1069, 1174, 1194
FauI	CCCGC	4 cut(s)	195, 227, 369, 378
FbaI	TGATCA	3 cut(s)	657, 733, 1171
Fnu4HI	GCNGC	9 cut(s)	276, 302, 314, 374, 428, 464, 568, 1242, 1345
FokI	GGATG	4 cut(s)	894, 962, 970, 1395
FriOI	GRGCYC	3 cut(s)	215, 241, 1209
Fsp4HI	GCNGC	9 cut(s)	276, 302, 314, 374, 428, 464, 568, 1242, 1345
FspBI	CTAG	3 cut(s)	609, 704, 980
GlaI	GCGC	2 cut(s)	300, 1291
GluI	GCNGC	9 cut(s)	276, 302, 314, 374, 428, 464, 568, 1242, 1345
GsuI	CTGGAG	1 cut(s)	308
HaeII	RGCGCY	1 cut(s)	302
HaeIII	GGCC	5 cut(s)	239, 365, 537, 834, 1116
HapII	CCGG	6 cut(s)	260, 539, 632, 665, 763, 1203
HgaI	GACGC	2 cut(s)	770, 800
HhaI	GCGC	2 cut(s)	301, 1292
Hin1I	GRCGYC	1 cut(s)	781
Hin1II	CATG	6 cut(s)	460, 839, 880, 1073, 1178, 1198
Hin6I	GCGC	2 cut(s)	299, 1290
HinP1I	GCGC	2 cut(s)	299, 1290
HincII	GTYRAC	3 cut(s)	454, 624, 1399
HindII	GTYRAC	3 cut(s)	454, 624, 1399
HinfI	GANTC	3 cut(s)	504, 867, 889
HpaI	GTTAAC	1 cut(s)	454
HpaII	CCGG	6 cut(s)	260, 539, 632, 665, 763, 1203
HphI	GGTGA	2 cut(s)	667, 1023
Hpy166II	GTNNAC	5 cut(s)	154, 343, 454, 624, 1399
Hpy188I	TCNGA	4 cut(s)	110, 133, 957, 1083
Hpy188III	TCNNGA	8 cut(s)	208, 287, 332, 763, 995, 1314, 1358, 1380
Hpy8I	GTNNAC	5 cut(s)	154, 343, 454, 624, 1399
Hpy99I	CGWCG	3 cut(s)	152, 233, 491
HpyAV	CCTTC	2 cut(s)	1293, 1381
HpyCH4III	ACNGT	3 cut(s)	710, 1065, 1255
HpyCH4IV	ACGT	2 cut(s)	345, 645
HpyCH4V	TGCA	7 cut(s)	169, 406, 415, 1039, 1091, 1337, 1417
HpyF10VI	GCNNNNNNNGC	7 cut(s)	179, 281, 290, 310, 412, 800, 1213
HpyF3I	CTNAG	3 cut(s)	279, 495, 1458
HpySE526I	ACGT	2 cut(s)	345, 645
Hsp92I	GRCGYC	1 cut(s)	781
Hsp92II	CATG	6 cut(s)	460, 839, 880, 1073, 1178, 1198
HspAI	GCGC	2 cut(s)	299, 1290
Kpn2I	TCCGGA	1 cut(s)	762
Ksp22I	TGATCA	3 cut(s)	657, 733, 1171
KspAI	GTTAAC	1 cut(s)	454
Kzo9I	GATC	8 cut(s)	247, 328, 432, 657, 733, 1120, 1171, 1279
LmnI	GCTCC	5 cut(s)	218, 765, 961, 1094, 1204
Lsp1109I	GCAGC	7 cut(s)	262, 288, 360, 414, 450, 579, 1253
LweI	GCATC	3 cut(s)	72, 1314, 1439
MaeI	CTAG	3 cut(s)	609, 704, 980
MaeII	ACGT	2 cut(s)	345, 645
MalI	GATC	8 cut(s)	249, 330, 434, 659, 735, 1122, 1173, 1281
MbiI	CCGCTC	2 cut(s)	182, 1344
MboI	GATC	8 cut(s)	247, 328, 432, 657, 733, 1120, 1171, 1279
MboII	GAAGA	7 cut(s)	404, 449, 608, 995, 1276, 1297, 1436
MhlI	GDGCHC	3 cut(s)	215, 241, 1209
MlsI	TGGCCA	1 cut(s)	834
MluCI	AATT	5 cut(s)	104, 421, 770, 894, 1099
MluNI	TGGCCA	1 cut(s)	834
MlyI	GAGTC	1 cut(s)	861
MmeI	TCCRAC	2 cut(s)	156, 1076
Mox20I	TGGCCA	1 cut(s)	834
MroI	TCCGGA	1 cut(s)	762
MscI	TGGCCA	1 cut(s)	834
MseI	TTAA	4 cut(s)	15, 453, 563, 1190
Msp20I	TGGCCA	1 cut(s)	834
MspA1I	CMGCKG	2 cut(s)	200, 373
MspI	CCGG	6 cut(s)	260, 539, 632, 665, 763, 1203
MspR9I	CCNGG	7 cut(s)	242, 539, 540, 632, 633, 1203, 1204
MvaI	CCWGG	1 cut(s)	242
MvnI	CGCG	1 cut(s)	1290
MwoI	GCNNNNNNNGC	7 cut(s)	179, 281, 290, 310, 412, 800, 1213
NciI	CCSGG	6 cut(s)	539, 540, 632, 633, 1203, 1204
NdeII	GATC	8 cut(s)	247, 328, 432, 657, 733, 1120, 1171, 1279
NlaIII	CATG	6 cut(s)	460, 839, 880, 1073, 1178, 1198
NlaIV	GGNNCC	7 cut(s)	238, 239, 258, 341, 511, 1200, 1318
NmeAIII	GCCGAG	1 cut(s)	39
NspI	RCATGY	2 cut(s)	460, 1073
PaeI	GCATGC	1 cut(s)	1073
PaeR7I	CTCGAG	1 cut(s)	332
PciI	ACATGT	1 cut(s)	456
PcsI	WCGNNNNNNNCGW	1 cut(s)	351
PfeI	GAWTC	2 cut(s)	504, 889
PflFI	GACNNNGTC	1 cut(s)	710
PkrI	GCNGC	9 cut(s)	277, 303, 315, 375, 429, 465, 569, 1243, 1346
PleI	GAGTC	1 cut(s)	861
PpsI	GAGTC	1 cut(s)	861
PpuMI	RGGWCCY	2 cut(s)	510, 976
PscI	ACATGT	1 cut(s)	456
PshAI	GACNNNNGTC	1 cut(s)	780
Psp124BI	GAGCTC	1 cut(s)	215
Psp5II	RGGWCCY	2 cut(s)	510, 976
Psp6I	CCWGG	1 cut(s)	240
PspGI	CCWGG	1 cut(s)	240
PspN4I	GGNNCC	7 cut(s)	238, 239, 258, 341, 511, 1200, 1318
PspOMI	GGGCCC	1 cut(s)	237
PspPI	GGNCC	9 cut(s)	237, 238, 340, 363, 510, 536, 976, 1049, 1114
PspPPI	RGGWCCY	2 cut(s)	510, 976
PsyI	GACNNNGTC	1 cut(s)	710
RsaI	GTAC	5 cut(s)	155, 401, 702, 825, 1062
RsaNI	GTAC	5 cut(s)	154, 400, 701, 824, 1061
SacI	GAGCTC	1 cut(s)	215
SaqAI	TTAA	4 cut(s)	15, 453, 563, 1190
SatI	GCNGC	9 cut(s)	276, 302, 314, 374, 428, 464, 568, 1242, 1345
Sau3AI	GATC	8 cut(s)	247, 328, 432, 657, 733, 1120, 1171, 1279
Sau96I	GGNCC	9 cut(s)	237, 238, 340, 363, 510, 536, 976, 1049, 1114
ScaI	AGTACT	2 cut(s)	401, 702
SchI	GAGTC	1 cut(s)	861
ScrFI	CCNGG	7 cut(s)	242, 539, 540, 632, 633, 1203, 1204
SduI	GDGCHC	3 cut(s)	215, 241, 1209
SfaNI	GCATC	3 cut(s)	72, 1314, 1439
SfcI	CTRYAG	1 cut(s)	739
Sfr274I	CTCGAG	1 cut(s)	332
SinI	GGWCC	4 cut(s)	340, 510, 976, 1049
SlaI	CTCGAG	1 cut(s)	332
SmaI	CCCGGG	3 cut(s)	540, 633, 1204
SmlI	CTYRAG	2 cut(s)	95, 332
SmoI	CTYRAG	2 cut(s)	95, 332
SphI	GCATGC	1 cut(s)	1073
Sse9I	AATT	5 cut(s)	104, 421, 770, 894, 1099
SspI	AATATT	1 cut(s)	1222
SspMI	CTAG	3 cut(s)	609, 704, 980
SstI	GAGCTC	1 cut(s)	215
StyD4I	CCNGG	7 cut(s)	240, 537, 538, 630, 631, 1201, 1202
StyI	CCWWGG	1 cut(s)	306
TaaI	ACNGT	3 cut(s)	710, 1065, 1255
TaiI	ACGT	2 cut(s)	348, 648
TaqI	TCGA	7 cut(s)	222, 246, 333, 435, 515, 754, 1209
TasI	AATT	5 cut(s)	104, 421, 770, 894, 1099
TatI	WGTACW	4 cut(s)	153, 399, 700, 1060
TauI	GCSGC	2 cut(s)	316, 1347
TfiI	GAWTC	2 cut(s)	504, 889
Tru1I	TTAA	4 cut(s)	15, 453, 563, 1190
Tru9I	TTAA	4 cut(s)	15, 453, 563, 1190
TscAI	CASTG	2 cut(s)	1070, 1258
TseI	GCWGC	7 cut(s)	275, 301, 373, 427, 463, 567, 1241
TspDTI	ATGAA	3 cut(s)	863, 896, 1297
TspGWI	ACGGA	1 cut(s)	136
TspMI	CCCGGG	3 cut(s)	538, 631, 1202
TspRI	CASTG	2 cut(s)	1070, 1258
Tth111I	GACNNNGTC	1 cut(s)	710
VpaK11BI	GGWCC	4 cut(s)	340, 510, 976, 1049
XagI	CCTNNNNNAGG	1 cut(s)	915
XapI	RAATTY	3 cut(s)	104, 770, 1099
XceI	RCATGY	2 cut(s)	460, 1073
XhoI	CTCGAG	1 cut(s)	332
XmaI	CCCGGG	3 cut(s)	538, 631, 1202
XspI	CTAG	3 cut(s)	609, 704, 980
ZrmI	AGTACT	2 cut(s)	401, 702

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.