Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr3

Orthogroup ID

OG0013918

Physical Location & Seq

Genomic Coordinates Forward (+)

7189992 .. 7192615

Gene Structure 2624 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000011158

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1437 bp

ATGTCTCTGACTATTAAGCTTCACGTCACCACTTCCCACCTCAACCTCAACATCACCGCAATCAAAAGACCAACCCTCCCCAGGTTAAGAACCACCAAACCCATTTACCATTCTGACTCATTATTGTCCCTCGTGTATAAGCAGAAACCCACAACAATGCATGAGCCGGCTCGATCCACAATCAAGAGCTCGCTGATCGAGCCGGACGGCGGGGCCTTGGTCGATCTCGTGGTGCCGGGAAGCGAGCGCGGGAGCAAGGCCCTCGAGGCCGAGTCGCTGCCGAAGGTGAGCCTCACCAAGATTGATCTGGAGTGGGTGCATGTGATCAGCGAGGGATGGGCTAGCCCTTTAAGAGGGTTCATGAGGGAGGACCAGTACTTGCAGAGCTTGCACTTCAATTCGTTGAGAGTCAAGGACGGGTCTGCGGTCAACATGTCGCTTCCCATTGTGTTGGCCATTGACGACGAGACCAAGGAGAGAATCGGGACGTCGCCGAATGTGGGGTTGGTTGGACCTAATGGAGATTTGATTGGGATATTAAGGAGCATTGAGATATACAAGCATAACAAAGAAGAAAGGATTGCGAGGACTTGGGGGTCAACTGCTTCAGGATTACCATACGTTGAGGAGGCCATCACTCCAGCTGGAAACTGGCTTATTGGCGGAGATTTAGAAGTACTAGAGCGTATCAAATACAACGATGGGCTCGATAACTACAGACTCTCTCCTCAACAACTTCGAAACGAATTTGATAGGCGCCAGGCGGATGCAGTTTTTGCATTTCAGTTGAGAAATCCTGTACATAATGGTCATGCTCTGTTGATGAATGATACACGCCAGCGACTCTTGGCAATGGGTTACAAGAATCCAATTCTATTGCTTCATCCTTTAGGAGGTTTCACAAAGCCGGATGATGTGCCCTTGGATGTTCGGATGGAGCAACATAGCAAGGTACTCGAGGATGGAGTTCTCGACCCTGAAACTACCATTGTGGGTATATTCCCATCACCTATGCACTATGCAGGTCCAACTGAGGTACAATGGCATGCAAAAGCACGGATAAATGCAGGCGCAAATTTTTACATTGTGGGGCGTGATCCTGCTGGTATGGGACACCCAACAGAGAAGAGAGACTTGTATGACCCTGATCATGGGAAAAAGGTGTTGAGCATGGCACCTGGCCTTGAGAAGCTCAATATTTTGCCATTCAGGGTGGCTGCTTATGACACTGTGGAGAAGAAGATGGCATTTTTTGATCCTTCACGTGCAAAAGATTTCCTCTTCATCTCTGGGACCAAGATGCGGACTTATGCAAAGACCGGCGAAAACCCTCCTGATGGTTTTATGTGTCCCGGAGGATGGAAGGTACTAGTGAACTACTACGAGAGCTTGCAAACAGAAGAGTCATCGCAGCAGCAAGCTGTTTTATCTACTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000103 GO:0003674 GO:0003824 GO:0004020 GO:0004779 GO:0004781 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006790 GO:0006793 GO:0006796 GO:0006950 GO:0007154 GO:0008150 GO:0008152 GO:0009267 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009970 GO:0009987 GO:0009991 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016779 GO:0031667 GO:0031668 GO:0031669 GO:0033554 GO:0042594 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0050896 GO:0051716 GO:0070566 GO:0071496

KEGG Pathways

Metabolic & Signaling

ko00230 ko00261 ko00450 ko00920 ko01100 ko01120 ko01130 map00230 map00261 map00450 map00920 map01100 map01120 map01130

Pfam Domains

Protein Families

ATP-sulfurylase PUA_2

Protein Analysis

479

Amino Acids

53.28

Weight (kDa)

6.75

Isoelectric Point (pI)

50.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PUA_2	PF14306	66 - 229	4e-42	PUA-like domain
ATP-sulfurylase	PF01747	238 - 459	3.7e-67	ATP-sulfurylase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0013918)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G19920
fragaria_vesca	FvH4_2g38710
malus_domestica	MD08G1014800.v1.1 MD15G1014100.v1.1
prunus_persica	Prupe.1G367700_v2.0.a1
pyrus_communis	pycom08g01320 pycom15g01220
rosa_chinensis	RchiOBHm_Chr6g0302601
rosa_laevigata	RLG00000011158
rosa_multiflora	Rmu_sc0005455.1_g000010
rosa_roxburghii	Rroxscaffold_7G00165530
rosa_rugosa	Rorug06G0315200
rosa_samantha	Rh6AG428600 Rh6BG473900 Rh6CG440800 Rh6DG427600
rosa_wichuraiana	Rw6G037090

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	595
AatII	GACGTC	1 cut(s)	491
AbsI	CCTCGAGG	1 cut(s)	263
Acc36I	ACCTGC	1 cut(s)	1013
AccB1I	GGYRCC	3 cut(s)	232, 756, 1174
AccII	CGCG	1 cut(s)	249
AciI	CCGC	7 cut(s)	57, 210, 249, 425, 663, 764, 1303
AclWI	GGATC	3 cut(s)	168, 1091, 1250
AcoI	YGGCCR	1 cut(s)	453
AcsI	RAATTY	2 cut(s)	746, 1075
AcuI	CTGAAG	1 cut(s)	591
AcvI	CACGTG	1 cut(s)	1265
AcyI	GRCGYC	2 cut(s)	488, 757
AfaI	GTAC	6 cut(s)	377, 678, 801, 954, 1038, 1368
AfiI	CCNNNNNNNGG	9 cut(s)	81, 209, 353, 500, 893, 1151, 1302, 1337, 1359
AflIII	ACRYGT	1 cut(s)	432
AgsI	TTSAA	1 cut(s)	397
AhlI	ACTAGT	1 cut(s)	1369
AjiI	CACGTC	1 cut(s)	25
AjnI	CCWGG	3 cut(s)	80, 759, 1177
AjuI	GAANNNNNNNTTGG	2 cut(s)	488, 520
AluBI	AGCT	7 cut(s)	19, 189, 387, 644, 1192, 1389, 1421
AluI	AGCT	7 cut(s)	19, 189, 387, 644, 1192, 1389, 1421
Alw21I	GWGCWC	1 cut(s)	191
Alw26I	GTCTC	3 cut(s)	9, 461, 1125
AlwI	GGATC	3 cut(s)	168, 1091, 1250
Ama87I	CYCGRG	2 cut(s)	263, 956
AoxI	GGCC	6 cut(s)	213, 258, 267, 453, 630, 1180
ApeKI	GCWGC	4 cut(s)	277, 1217, 1411, 1414
ApoI	RAATTY	2 cut(s)	746, 1075
AspLEI	GCGC	3 cut(s)	249, 759, 1073
AspS9I	GGNCC	6 cut(s)	213, 259, 370, 512, 1025, 1293
AsuC2I	CCSGG	2 cut(s)	237, 1353
AsuHPI	GGTGA	5 cut(s)	19, 46, 286, 298, 999
AsuII	TTCGAA	1 cut(s)	739
AsuNHI	GCTAGC	1 cut(s)	341
AvaI	CYCGRG	2 cut(s)	263, 956
AvaII	GGWCC	4 cut(s)	370, 512, 1025, 1293
BaeGI	GKGCMC	1 cut(s)	921
BalI	TGGCCA	1 cut(s)	455
BanI	GGYRCC	3 cut(s)	232, 756, 1174
BanII	GRGCYC	2 cut(s)	191, 708
BauI	CACGAG	2 cut(s)	131, 227
BbrPI	CACGTG	1 cut(s)	1265
Bbv12I	GWGCWC	1 cut(s)	191
BbvI	GCAGC	4 cut(s)	264, 1204, 1423, 1426
BccI	CCATC	9 cut(s)	330, 641, 695, 928, 956, 1012, 1237, 1331, 1353
BceAI	ACGGC	1 cut(s)	223
BcgI	CGANNNNNNTGC	4 cut(s)	244, 278, 937, 971
BciT130I	CCWGG	3 cut(s)	82, 761, 1179
BclI	TGATCA	2 cut(s)	324, 1147
BcnI	CCSGG	2 cut(s)	237, 1353
BcoDI	GTCTC	3 cut(s)	9, 461, 1125
BcuI	ACTAGT	1 cut(s)	1369
BfaI	CTAG	3 cut(s)	342, 680, 1370
BfmI	CTRYAG	1 cut(s)	715
BfoI	RGCGCY	1 cut(s)	760
BfuAI	ACCTGC	1 cut(s)	1013
BglI	GCCNNNNNGGC	1 cut(s)	266
BisI	GCNGC	4 cut(s)	278, 1218, 1412, 1415
BlsI	GCNGC	4 cut(s)	279, 1219, 1413, 1416
BmcAI	AGTACT	2 cut(s)	377, 678
Bme1390I	CCNGG	5 cut(s)	82, 237, 761, 1179, 1353
Bme18I	GGWCC	4 cut(s)	370, 512, 1025, 1293
BmeT110I	CYCGRG	2 cut(s)	263, 956
BmgBI	CACGTC	1 cut(s)	25
BmgT120I	GGNCC	6 cut(s)	213, 259, 370, 512, 1025, 1293
BmiI	GGNNCC	5 cut(s)	214, 234, 758, 1176, 1294
BmrFI	CCNGG	5 cut(s)	82, 237, 761, 1179, 1353
BmsI	GCATC	2 cut(s)	757, 1290
BmtI	GCTAGC	1 cut(s)	345
BpmI	CTGGAG	2 cut(s)	329, 624
Bpu14I	TTCGAA	1 cut(s)	739
BpuEI	CTTGAG	1 cut(s)	1205
BpuMI	CCSGG	2 cut(s)	237, 1353
BsaAI	YACGTR	1 cut(s)	1265
BsaHI	GRCGYC	2 cut(s)	488, 757
BsaI	GGTCTC	1 cut(s)	461
BsaJI	CCNNGG	4 cut(s)	80, 216, 471, 921
BsaXI	ACNNNNNCTCC	4 cut(s)	60, 90, 359, 389
Bsc4I	CCNNNNNNNGG	9 cut(s)	81, 209, 353, 500, 893, 1151, 1302, 1337, 1359
Bse118I	RCCGGY	2 cut(s)	166, 1319
Bse1I	ACTGG	2 cut(s)	373, 656
Bse3DI	GCAATG	1 cut(s)	858
BseBI	CCWGG	3 cut(s)	82, 761, 1179
BseDI	CCNNGG	4 cut(s)	80, 216, 471, 921
BseGI	GGATG	8 cut(s)	341, 772, 883, 916, 931, 939, 967, 1364
BseLI	CCNNNNNNNGG	9 cut(s)	81, 209, 353, 500, 893, 1151, 1302, 1337, 1359
BseMI	GCAATG	1 cut(s)	858
BseMII	CTCAG	1 cut(s)	1023
BseNI	ACTGG	2 cut(s)	373, 656
BseRI	GAGGAG	2 cut(s)	641, 717
BseSI	GKGCMC	1 cut(s)	921
BseXI	GCAGC	4 cut(s)	264, 1204, 1423, 1426
Bsh1236I	CGCG	1 cut(s)	249
BshFI	GGCC	6 cut(s)	215, 260, 269, 455, 632, 1182
BshNI	GGYRCC	3 cut(s)	232, 756, 1174
BsiHKAI	GWGCWC	1 cut(s)	191
BsiHKCI	CYCGRG	2 cut(s)	263, 956
BsiSI	CCGG	6 cut(s)	167, 203, 236, 908, 1320, 1353
BslFI	GGGAC	5 cut(s)	112, 499, 1125, 1306, 1335
BslI	CCNNNNNNNGG	9 cut(s)	81, 209, 353, 500, 893, 1151, 1302, 1337, 1359
BsmAI	GTCTC	3 cut(s)	9, 461, 1125
BsmFI	GGGAC	5 cut(s)	112, 499, 1125, 1306, 1335
BsnI	GGCC	6 cut(s)	215, 260, 269, 455, 632, 1182
Bso31I	GGTCTC	1 cut(s)	461
BsoBI	CYCGRG	2 cut(s)	263, 956
Bsp119I	TTCGAA	1 cut(s)	739
Bsp1286I	GDGCHC	3 cut(s)	191, 708, 921
Bsp1407I	TGTACA	1 cut(s)	799
Bsp143I	GATC	8 cut(s)	173, 195, 223, 304, 324, 1096, 1147, 1255
BspACI	CCGC	7 cut(s)	57, 210, 249, 425, 663, 764, 1303
BspANI	GGCC	6 cut(s)	215, 260, 269, 455, 632, 1182
BspCNI	CTCAG	1 cut(s)	1024
BspFNI	CGCG	1 cut(s)	249
BspHI	TCATGA	1 cut(s)	360
BspLI	GGNNCC	5 cut(s)	214, 234, 758, 1176, 1294
BspMI	ACCTGC	1 cut(s)	1013
BspOI	GCTAGC	1 cut(s)	345
BspPI	GGATC	3 cut(s)	168, 1091, 1250
BspT104I	TTCGAA	1 cut(s)	739
BspT107I	GGYRCC	3 cut(s)	232, 756, 1174
BspTNI	GGTCTC	1 cut(s)	461
BsrDI	GCAATG	1 cut(s)	858
BsrFI	RCCGGY	2 cut(s)	166, 1319
BsrGI	TGTACA	1 cut(s)	799
BsrI	ACTGG	2 cut(s)	373, 656
BssAI	RCCGGY	2 cut(s)	166, 1319
BssECI	CCNNGG	4 cut(s)	80, 216, 471, 921
BssMI	GATC	8 cut(s)	173, 195, 223, 304, 324, 1096, 1147, 1255
BssNI	GRCGYC	2 cut(s)	488, 757
BssSI	CACGAG	2 cut(s)	131, 227
BssT1I	CCWWGG	3 cut(s)	216, 471, 921
Bst2BI	CACGAG	2 cut(s)	131, 227
Bst2UI	CCWGG	3 cut(s)	82, 761, 1179
Bst4CI	ACNGT	1 cut(s)	1231
Bst6I	CTCTTC	3 cut(s)	1121, 1286, 1395
BstACI	GRCGYC	2 cut(s)	488, 757
BstAPI	GCANNNNNTGC	2 cut(s)	388, 776
BstAUI	TGTACA	1 cut(s)	799
BstBAI	YACGTR	1 cut(s)	1265
BstBI	TTCGAA	1 cut(s)	739
BstDEI	CTNAG	2 cut(s)	1032, 1434
BstENI	CCTNNNNNAGG	2 cut(s)	351, 891
BstF5I	GGATG	8 cut(s)	341, 772, 883, 916, 931, 939, 967, 1364
BstFNI	CGCG	1 cut(s)	249
BstH2I	RGCGCY	1 cut(s)	760
BstHHI	GCGC	3 cut(s)	249, 759, 1073
BstKTI	GATC	8 cut(s)	176, 198, 226, 307, 327, 1099, 1150, 1258
BstMAI	GTCTC	3 cut(s)	9, 461, 1125
BstMBI	GATC	8 cut(s)	173, 195, 223, 304, 324, 1096, 1147, 1255
BstMWI	GCNNNNNNNGC	4 cut(s)	199, 266, 388, 776
BstNI	CCWGG	3 cut(s)	82, 761, 1179
BstNSI	RCATGY	3 cut(s)	323, 436, 1049
BstSCI	CCNGG	5 cut(s)	80, 235, 759, 1177, 1351
BstSFI	CTRYAG	1 cut(s)	715
BstSLI	GKGCMC	1 cut(s)	921
BstUI	CGCG	1 cut(s)	249
BstV1I	GCAGC	4 cut(s)	264, 1204, 1423, 1426
BstXI	CCANNNNNNTGG	1 cut(s)	451
BsuRI	GGCC	6 cut(s)	215, 260, 269, 455, 632, 1182
BtgZI	GCGATG	1 cut(s)	1392
BtrI	CACGTC	1 cut(s)	25
BtsCI	GGATG	8 cut(s)	341, 772, 883, 916, 931, 939, 967, 1364
BtsIMutI	CAGTG	1 cut(s)	1227
BveI	ACCTGC	1 cut(s)	1013
CciI	TCATGA	1 cut(s)	360
CfoI	GCGC	3 cut(s)	249, 759, 1073
Cfr10I	RCCGGY	2 cut(s)	166, 1319
Cfr13I	GGNCC	6 cut(s)	213, 259, 370, 512, 1025, 1293
Csp6I	GTAC	6 cut(s)	376, 677, 800, 953, 1037, 1367
CviAII	CATG	8 cut(s)	161, 320, 361, 433, 812, 1046, 1151, 1171
CviQI	GTAC	6 cut(s)	376, 677, 800, 953, 1037, 1367
DdeI	CTNAG	2 cut(s)	1032, 1434
DinI	GGCGCC	1 cut(s)	758
DpnI	GATC	8 cut(s)	175, 197, 225, 306, 326, 1098, 1149, 1257
DpnII	GATC	8 cut(s)	173, 195, 223, 304, 324, 1096, 1147, 1255
DrdI	GACNNNNNNGTC	1 cut(s)	595
DseDI	GACNNNNNNGTC	1 cut(s)	595
EaeI	YGGCCR	1 cut(s)	453
Eam1104I	CTCTTC	3 cut(s)	1121, 1286, 1395
EarI	CTCTTC	3 cut(s)	1121, 1286, 1395
EciI	GGCGGA	2 cut(s)	678, 779
Ecl136II	GAGCTC	1 cut(s)	189
Eco130I	CCWWGG	3 cut(s)	216, 471, 921
Eco24I	GRGCYC	2 cut(s)	191, 708
Eco31I	GGTCTC	1 cut(s)	461
Eco47I	GGWCC	4 cut(s)	370, 512, 1025, 1293
Eco53kI	GAGCTC	1 cut(s)	189
Eco57I	CTGAAG	1 cut(s)	591
Eco72I	CACGTG	1 cut(s)	1265
Eco88I	CYCGRG	2 cut(s)	263, 956
EcoICRI	GAGCTC	1 cut(s)	189
EcoNI	CCTNNNNNAGG	2 cut(s)	351, 891
EcoO109I	RGGNCCY	2 cut(s)	213, 259
EcoRII	CCWGG	3 cut(s)	80, 759, 1177
EcoT14I	CCWWGG	3 cut(s)	216, 471, 921
EcoT22I	ATGCAT	1 cut(s)	162
EcoT38I	GRGCYC	2 cut(s)	191, 708
EgeI	GGCGCC	1 cut(s)	758
EheI	GGCGCC	1 cut(s)	758
ErhI	CCWWGG	3 cut(s)	216, 471, 921
FaeI	CATG	8 cut(s)	164, 323, 364, 436, 815, 1049, 1154, 1174
FalI	AAGNNNNNCTT	2 cut(s)	1118, 1150
FaqI	GGGAC	5 cut(s)	112, 499, 1125, 1306, 1335
FatI	CATG	8 cut(s)	160, 319, 360, 432, 811, 1045, 1150, 1170
FauI	CCCGC	2 cut(s)	203, 242
FbaI	TGATCA	2 cut(s)	324, 1147
Fnu4HI	GCNGC	4 cut(s)	278, 1218, 1412, 1415
FokI	GGATG	8 cut(s)	348, 779, 870, 923, 938, 946, 974, 1371
FriOI	GRGCYC	2 cut(s)	191, 708
Fsp4HI	GCNGC	4 cut(s)	278, 1218, 1412, 1415
FspBI	CTAG	3 cut(s)	342, 680, 1370
GlaI	GCGC	3 cut(s)	248, 758, 1072
GluI	GCNGC	4 cut(s)	278, 1218, 1412, 1415
GsuI	CTGGAG	2 cut(s)	329, 624
HaeII	RGCGCY	1 cut(s)	760
HaeIII	GGCC	6 cut(s)	215, 260, 269, 455, 632, 1182
HapII	CCGG	6 cut(s)	167, 203, 236, 908, 1320, 1353
HhaI	GCGC	3 cut(s)	249, 759, 1073
Hin1I	GRCGYC	2 cut(s)	488, 757
Hin1II	CATG	8 cut(s)	164, 323, 364, 436, 815, 1049, 1154, 1174
Hin6I	GCGC	3 cut(s)	247, 757, 1071
HinP1I	GCGC	3 cut(s)	247, 757, 1071
HincII	GTYRAC	2 cut(s)	430, 600
HindII	GTYRAC	2 cut(s)	430, 600
HindIII	AAGCTT	1 cut(s)	17
HinfI	GANTC	8 cut(s)	116, 272, 408, 480, 720, 843, 865, 1403
HpaII	CCGG	6 cut(s)	167, 203, 236, 908, 1320, 1353
HphI	GGTGA	5 cut(s)	19, 46, 286, 298, 999
Hpy166II	GTNNAC	3 cut(s)	430, 600, 1375
Hpy188I	TCNGA	3 cut(s)	9, 115, 933
Hpy188III	TCNNGA	7 cut(s)	184, 308, 361, 484, 609, 971, 1334
Hpy8I	GTNNAC	3 cut(s)	430, 600, 1375
Hpy99I	CGWCG	2 cut(s)	467, 493
HpyAV	CCTTC	3 cut(s)	277, 1269, 1357
HpyCH4III	ACNGT	1 cut(s)	1231
HpyCH4IV	ACGT	4 cut(s)	24, 488, 621, 1264
HpyF10VI	GCNNNNNNNGC	4 cut(s)	199, 266, 388, 776
HpyF3I	CTNAG	2 cut(s)	1032, 1434
HpySE526I	ACGT	4 cut(s)	24, 488, 621, 1264
Hsp92I	GRCGYC	2 cut(s)	488, 757
Hsp92II	CATG	8 cut(s)	164, 323, 364, 436, 815, 1049, 1154, 1174
HspAI	GCGC	3 cut(s)	247, 757, 1071
KasI	GGCGCC	1 cut(s)	756
KroI	GCCGGC	1 cut(s)	166
KroNI	GCCGGC	1 cut(s)	168
Ksp22I	TGATCA	2 cut(s)	324, 1147
Kzo9I	GATC	8 cut(s)	173, 195, 223, 304, 324, 1096, 1147, 1255
LmnI	GCTCC	3 cut(s)	252, 543, 937
Lsp1109I	GCAGC	4 cut(s)	264, 1204, 1423, 1426
LweI	GCATC	2 cut(s)	757, 1290
MaeI	CTAG	3 cut(s)	342, 680, 1370
MaeII	ACGT	4 cut(s)	24, 488, 621, 1264
MaeIII	GTNAC	2 cut(s)	25, 857
MalI	GATC	8 cut(s)	175, 197, 225, 306, 326, 1098, 1149, 1257
MboI	GATC	8 cut(s)	173, 195, 223, 304, 324, 1096, 1147, 1255
MboII	GAAGA	6 cut(s)	584, 1138, 1249, 1252, 1273, 1412
MhlI	GDGCHC	3 cut(s)	191, 708, 921
MlsI	TGGCCA	1 cut(s)	455
MluCI	AATT	4 cut(s)	397, 746, 870, 1075
MluNI	TGGCCA	1 cut(s)	455
Mly113I	GGCGCC	1 cut(s)	757
MlyI	GAGTC	6 cut(s)	110, 281, 417, 714, 837, 1412
MmeI	TCCRAC	2 cut(s)	490, 1052
Mox20I	TGGCCA	1 cut(s)	455
Mph1103I	ATGCAT	1 cut(s)	162
MroNI	GCCGGC	1 cut(s)	166
MscI	TGGCCA	1 cut(s)	455
MseI	TTAA	4 cut(s)	15, 86, 350, 539
MslI	CAYNNNNRTG	1 cut(s)	155
Msp20I	TGGCCA	1 cut(s)	455
MspA1I	CMGCKG	1 cut(s)	644
MspI	CCGG	6 cut(s)	167, 203, 236, 908, 1320, 1353
MspR9I	CCNGG	5 cut(s)	82, 237, 761, 1179, 1353
MvaI	CCWGG	3 cut(s)	82, 761, 1179
MvnI	CGCG	1 cut(s)	249
MwoI	GCNNNNNNNGC	4 cut(s)	199, 266, 388, 776
NaeI	GCCGGC	1 cut(s)	168
NarI	GGCGCC	1 cut(s)	757
NciI	CCSGG	2 cut(s)	237, 1353
NdeII	GATC	8 cut(s)	173, 195, 223, 304, 324, 1096, 1147, 1255
NgoMIV	GCCGGC	1 cut(s)	166
NheI	GCTAGC	1 cut(s)	341
NlaIII	CATG	8 cut(s)	164, 323, 364, 436, 815, 1049, 1154, 1174
NlaIV	GGNNCC	5 cut(s)	214, 234, 758, 1176, 1294
NmeAIII	GCCGAG	1 cut(s)	295
NmuCI	GTSAC	1 cut(s)	25
NsiI	ATGCAT	1 cut(s)	162
NspI	RCATGY	3 cut(s)	323, 436, 1049
NspV	TTCGAA	1 cut(s)	739
PaeI	GCATGC	1 cut(s)	1049
PaeR7I	CTCGAG	2 cut(s)	263, 956
PagI	TCATGA	1 cut(s)	360
PciI	ACATGT	1 cut(s)	432
PcsI	WCGNNNNNNNCGW	1 cut(s)	705
PdiI	GCCGGC	1 cut(s)	168
PfeI	GAWTC	2 cut(s)	480, 865
PfoI	TCCNGGA	1 cut(s)	1351
PkrI	GCNGC	4 cut(s)	279, 1219, 1413, 1416
PleI	GAGTC	6 cut(s)	110, 280, 416, 714, 837, 1411
PluTI	GGCGCC	1 cut(s)	760
PmaCI	CACGTG	1 cut(s)	1265
PmlI	CACGTG	1 cut(s)	1265
PpsI	GAGTC	6 cut(s)	110, 280, 416, 714, 837, 1411
Ppu21I	YACGTR	1 cut(s)	1265
PscI	ACATGT	1 cut(s)	432
Psp124BI	GAGCTC	1 cut(s)	191
Psp6I	CCWGG	3 cut(s)	80, 759, 1177
PspCI	CACGTG	1 cut(s)	1265
PspGI	CCWGG	3 cut(s)	80, 759, 1177
PspN4I	GGNNCC	5 cut(s)	214, 234, 758, 1176, 1294
PspPI	GGNCC	6 cut(s)	213, 259, 370, 512, 1025, 1293
PspXI	VCTCGAGB	2 cut(s)	263, 956
PvuII	CAGCTG	1 cut(s)	644
RsaI	GTAC	6 cut(s)	377, 678, 801, 954, 1038, 1368
RsaNI	GTAC	6 cut(s)	376, 677, 800, 953, 1037, 1367
RseI	CAYNNNNRTG	1 cut(s)	155
SacI	GAGCTC	1 cut(s)	191
SaqAI	TTAA	4 cut(s)	15, 86, 350, 539
SatI	GCNGC	4 cut(s)	278, 1218, 1412, 1415
Sau3AI	GATC	8 cut(s)	173, 195, 223, 304, 324, 1096, 1147, 1255
Sau96I	GGNCC	6 cut(s)	213, 259, 370, 512, 1025, 1293
ScaI	AGTACT	2 cut(s)	377, 678
SchI	GAGTC	6 cut(s)	110, 281, 417, 714, 837, 1412
ScrFI	CCNGG	5 cut(s)	82, 237, 761, 1179, 1353
SduI	GDGCHC	3 cut(s)	191, 708, 921
SfaNI	GCATC	2 cut(s)	757, 1290
SfcI	CTRYAG	1 cut(s)	715
SfiI	GGCCNNNNNGGCC	1 cut(s)	266
SfoI	GGCGCC	1 cut(s)	758
Sfr274I	CTCGAG	2 cut(s)	263, 956
SfuI	TTCGAA	1 cut(s)	739
SinI	GGWCC	4 cut(s)	370, 512, 1025, 1293
SlaI	CTCGAG	2 cut(s)	263, 956
SmiMI	CAYNNNNRTG	1 cut(s)	155
SmlI	CTYRAG	3 cut(s)	263, 956, 1184
SmoI	CTYRAG	3 cut(s)	263, 956, 1184
SpeI	ACTAGT	1 cut(s)	1369
SphI	GCATGC	1 cut(s)	1049
Sse9I	AATT	4 cut(s)	397, 746, 870, 1075
SsiI	CCGC	7 cut(s)	57, 210, 249, 425, 663, 764, 1303
SspDI	GGCGCC	1 cut(s)	756
SspI	AATATT	1 cut(s)	1198
SspMI	CTAG	3 cut(s)	342, 680, 1370
SstI	GAGCTC	1 cut(s)	191
StyD4I	CCNGG	5 cut(s)	80, 235, 759, 1177, 1351
StyI	CCWWGG	3 cut(s)	216, 471, 921
TaaI	ACNGT	1 cut(s)	1231
TaiI	ACGT	4 cut(s)	27, 491, 624, 1267
TaqI	TCGA	8 cut(s)	172, 198, 222, 264, 708, 739, 957, 972
TasI	AATT	4 cut(s)	397, 746, 870, 1075
TatI	WGTACW	3 cut(s)	375, 676, 799
TfiI	GAWTC	2 cut(s)	480, 865
Tru1I	TTAA	4 cut(s)	15, 86, 350, 539
Tru9I	TTAA	4 cut(s)	15, 86, 350, 539
TscAI	CASTG	1 cut(s)	1234
TseFI	GTSAC	1 cut(s)	25
TseI	GCWGC	4 cut(s)	277, 1217, 1411, 1414
Tsp45I	GTSAC	1 cut(s)	25
TspDTI	ATGAA	4 cut(s)	349, 839, 872, 1273
TspGWI	ACGGA	1 cut(s)	1072
TspRI	CASTG	1 cut(s)	1234
VpaK11BI	GGWCC	4 cut(s)	370, 512, 1025, 1293
XagI	CCTNNNNNAGG	2 cut(s)	351, 891
XapI	RAATTY	2 cut(s)	746, 1075
XceI	RCATGY	3 cut(s)	323, 436, 1049
XcmI	CCANNNNNNNNNTGG	2 cut(s)	304, 648
XhoI	CTCGAG	2 cut(s)	263, 956
XspI	CTAG	3 cut(s)	342, 680, 1370
ZraI	GACGTC	1 cut(s)	489
ZrmI	AGTACT	2 cut(s)	377, 678
Zsp2I	ATGCAT	1 cut(s)	162

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.