Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0013918

Physical Location & Seq

Genomic Coordinates Reverse (-)

62678545 .. 62681650

Gene Structure 3106 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ27181

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1431 bp

ATGTCTCTGACTATTAAGCTTCACGTTACCACCTCCCACCTCAACATCACCGCAATCAAAAGACCAACCCTCCCCAGGTTAAGAACCACCAAACCCATTTACCATTCTGACGCATTATTGTGCCTCGTGTATAAACAGAAACCCACAACAATGCATGAGCCGGCTCGATCCACCATCAAGAGCTCGCTGATCGAGCCGGACGGCGGGTCCTTGGTCGATCTCGTGGTGCCGGAAAGCGAGCGCGGGAGCAAGGCCCTCGAGGCCGAGTCGCTGCCGAAGTTGAGCCTGACCAAGATTGATCTGGAGTGGGTGCATGTGATCAGCGAGGGATGGGCTAGCCCTTTAAGAGGGTTCATGAGGGAGGACCAGTACTTGCAGAGCTTGCACTTCAATTCGTTGAGAGTCAAGGACGGGTCTGTGGTCAACATGTCGCTTCCCATTGTGTTGGCCATTGACGACGAGACCAAGGAGAGAATCGGGACGTCGCCGAATGTGGGGTTGGTTGGACCTAATGGAGATTTGATTGGGATATTAAGGAGCATTGAGATATACAAGCATAACAAAGAAGAAAGGATTGCGAGGACTTGGGGGTCAACTGCACCAGGATTGCCGTACGTTGAAGAGGCCATCACTCCAGCTGGAAACTGGCTTATTGGCGGAGATTTAGAAGTACTAGAGCGTATCAAATACAATGATGGGCTCGATAACTACAGACTCTCTCCTCAACAACTCCGAAACGAATTTGATAAGCGCCAGGCGGACGCAGTTTTTGCGTTTCAGTTGAGAAATCCTGTACATAATGGTCATGCTCTGTTGATGAATGATACACGCCGGCGACTCTTGGAAATGGGTTACAAGAATCCAATTCTATTGCTTCATCCTTTAGGAGGTTTCACAAAGCCGGATGATGTGCCCTTGGATGTTCGGATGGAGCAGCATAGCAAGGTACTAGAGGATGGAGTTCTCGACCCTGAAACTACCATTGTGGGCATATTCCCATCACCTATGCACTATGCAGGTCCAACTGAGGTACAATGGCATGCAAAAGCACGGATAAATGCAGGCGCAAATTTTTACATTGTGGGGCGTGATCCTGCTGGTATGGGACACCCAACAGAGAAGAGAGACTTGTATGACCCTGATCATGGGAAAAAGGTGTTGAGCATGGCACCTGGCCTTGAGAAGCTCAATATTTTGCCATTCAGGGTGGCTGCTTATGACACTGTGGAGAAGAAGATGGCATTTTTTGATCCTTCACGTGCAAAAGATTTCCTCTTCATCTCTGGGACCAAGATGCGGACTTATGCAAAGACCGGCGAAAACCCTCCTGATGGTTTTATGTGTCCCGGAGGATGGAAGGTTCTAGTGAACTACTACGAGAGCTTGCAAACAGAAGAGTCATCGCAGCACCAAGCTGTTTTATCTACTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000103 GO:0003674 GO:0003824 GO:0004020 GO:0004779 GO:0004781 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006790 GO:0006793 GO:0006796 GO:0006950 GO:0007154 GO:0008150 GO:0008152 GO:0009267 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009970 GO:0009987 GO:0009991 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016779 GO:0031667 GO:0031668 GO:0031669 GO:0033554 GO:0042594 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0050896 GO:0051716 GO:0070566 GO:0071496

KEGG Pathways

Metabolic & Signaling

ko00230 ko00261 ko00450 ko00920 ko01100 ko01120 ko01130 map00230 map00261 map00450 map00920 map01100 map01120 map01130

Pfam Domains

Protein Families

ATP-sulfurylase PUA_2

Protein Analysis

476

Amino Acids

53.26

Weight (kDa)

6.61

Isoelectric Point (pI)

52.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PUA_2	PF14306	64 - 227	6.1e-43	PUA-like domain
ATP-sulfurylase	PF01747	236 - 457	2.3e-67	ATP-sulfurylase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013918)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G19920
fragaria_vesca	FvH4_2g38710
malus_domestica	MD08G1014800.v1.1 MD15G1014100.v1.1
prunus_persica	Prupe.1G367700_v2.0.a1
pyrus_communis	pycom08g01320 pycom15g01220
rosa_chinensis	RchiOBHm_Chr6g0302601
rosa_laevigata	RLG00000011158
rosa_multiflora	Rmu_sc0005455.1_g000010
rosa_roxburghii	Rroxscaffold_7G00165530
rosa_rugosa	Rorug06G0315200
rosa_samantha	Rh6AG428600 Rh6BG473900 Rh6CG440800 Rh6DG427600
rosa_wichuraiana	Rw6G037090

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	589
AatII	GACGTC	1 cut(s)	485
AbsI	CCTCGAGG	1 cut(s)	257
Acc36I	ACCTGC	1 cut(s)	1007
AccB1I	GGYRCC	2 cut(s)	226, 1168
AccII	CGCG	1 cut(s)	243
AciI	CCGC	6 cut(s)	51, 204, 243, 657, 758, 1297
AclWI	GGATC	3 cut(s)	162, 1085, 1244
AcoI	YGGCCR	1 cut(s)	447
AcsI	RAATTY	2 cut(s)	740, 1069
AcvI	CACGTG	1 cut(s)	1259
AcyI	GRCGYC	1 cut(s)	482
AfaI	GTAC	6 cut(s)	371, 614, 672, 795, 948, 1032
AfiI	CCNNNNNNNGG	9 cut(s)	75, 203, 347, 494, 887, 1145, 1296, 1331, 1353
AflIII	ACRYGT	1 cut(s)	426
AgsI	TTSAA	2 cut(s)	391, 620
AhdI	GACNNNNNGTC	1 cut(s)	205
AjnI	CCWGG	4 cut(s)	74, 601, 753, 1171
AjuI	GAANNNNNNNTTGG	2 cut(s)	482, 514
AloI	GAACNNNNNNTCC	2 cut(s)	1344, 1376
AluBI	AGCT	7 cut(s)	19, 183, 381, 638, 1186, 1383, 1415
AluI	AGCT	7 cut(s)	19, 183, 381, 638, 1186, 1383, 1415
Alw21I	GWGCWC	1 cut(s)	185
Alw26I	GTCTC	3 cut(s)	9, 455, 1119
AlwI	GGATC	3 cut(s)	162, 1085, 1244
Ama87I	CYCGRG	1 cut(s)	257
AoxI	GGCC	5 cut(s)	252, 261, 447, 624, 1174
ApeKI	GCWGC	4 cut(s)	271, 934, 1211, 1405
ApoI	RAATTY	2 cut(s)	740, 1069
ArsI	GACNNNNNNTTYG	2 cut(s)	752, 784
AspLEI	GCGC	3 cut(s)	243, 753, 1067
AspS9I	GGNCC	6 cut(s)	207, 253, 364, 506, 1019, 1287
AsuC2I	CCSGG	1 cut(s)	1347
AsuHPI	GGTGA	2 cut(s)	40, 993
AsuNHI	GCTAGC	1 cut(s)	335
AvaI	CYCGRG	1 cut(s)	257
AvaII	GGWCC	5 cut(s)	207, 364, 506, 1019, 1287
BaeGI	GKGCMC	1 cut(s)	915
BalI	TGGCCA	1 cut(s)	449
BanI	GGYRCC	2 cut(s)	226, 1168
BanII	GRGCYC	2 cut(s)	185, 702
BauI	CACGAG	2 cut(s)	125, 221
BbrPI	CACGTG	1 cut(s)	1259
Bbv12I	GWGCWC	1 cut(s)	185
BbvI	GCAGC	4 cut(s)	258, 946, 1198, 1417
BceAI	ACGGC	2 cut(s)	217, 595
BcgI	CGANNNNNNTGC	2 cut(s)	238, 272
BciT130I	CCWGG	4 cut(s)	76, 603, 755, 1173
BclI	TGATCA	2 cut(s)	318, 1141
BcnI	CCSGG	1 cut(s)	1347
BcoDI	GTCTC	3 cut(s)	9, 455, 1119
BfaI	CTAG	4 cut(s)	336, 674, 950, 1364
BfmI	CTRYAG	1 cut(s)	709
BfoI	RGCGCY	1 cut(s)	754
BfuAI	ACCTGC	1 cut(s)	1007
BglI	GCCNNNNNGGC	1 cut(s)	260
BisI	GCNGC	4 cut(s)	272, 935, 1212, 1406
BlsI	GCNGC	4 cut(s)	273, 936, 1213, 1407
BmcAI	AGTACT	2 cut(s)	371, 672
Bme1390I	CCNGG	5 cut(s)	76, 603, 755, 1173, 1347
Bme18I	GGWCC	5 cut(s)	207, 364, 506, 1019, 1287
BmeRI	GACNNNNNGTC	1 cut(s)	205
BmeT110I	CYCGRG	1 cut(s)	257
BmgT120I	GGNCC	6 cut(s)	207, 253, 364, 506, 1019, 1287
BmiI	GGNNCC	4 cut(s)	208, 228, 1170, 1288
BmrFI	CCNGG	5 cut(s)	76, 603, 755, 1173, 1347
BmsI	GCATC	1 cut(s)	1284
BmtI	GCTAGC	1 cut(s)	339
BpmI	CTGGAG	2 cut(s)	323, 618
BpuEI	CTTGAG	1 cut(s)	1199
BpuMI	CCSGG	1 cut(s)	1347
BsaAI	YACGTR	1 cut(s)	1259
BsaHI	GRCGYC	1 cut(s)	482
BsaI	GGTCTC	1 cut(s)	455
BsaJI	CCNNGG	4 cut(s)	74, 210, 465, 915
BsaXI	ACNNNNNCTCC	4 cut(s)	54, 84, 353, 383
Bsc4I	CCNNNNNNNGG	9 cut(s)	75, 203, 347, 494, 887, 1145, 1296, 1331, 1353
Bse118I	RCCGGY	3 cut(s)	160, 831, 1313
Bse1I	ACTGG	2 cut(s)	367, 650
BseBI	CCWGG	4 cut(s)	76, 603, 755, 1173
BseDI	CCNNGG	4 cut(s)	74, 210, 465, 915
BseGI	GGATG	7 cut(s)	335, 877, 910, 925, 933, 961, 1358
BseLI	CCNNNNNNNGG	9 cut(s)	75, 203, 347, 494, 887, 1145, 1296, 1331, 1353
BseMII	CTCAG	1 cut(s)	1017
BseNI	ACTGG	2 cut(s)	367, 650
BseRI	GAGGAG	1 cut(s)	711
BseSI	GKGCMC	1 cut(s)	915
BseXI	GCAGC	4 cut(s)	258, 946, 1198, 1417
BsgI	GTGCAG	1 cut(s)	582
Bsh1236I	CGCG	1 cut(s)	243
BshFI	GGCC	5 cut(s)	254, 263, 449, 626, 1176
BshNI	GGYRCC	2 cut(s)	226, 1168
BsiHKAI	GWGCWC	1 cut(s)	185
BsiHKCI	CYCGRG	1 cut(s)	257
BsiSI	CCGG	7 cut(s)	161, 197, 230, 832, 902, 1314, 1347
BsiWI	CGTACG	1 cut(s)	612
BslFI	GGGAC	4 cut(s)	493, 1119, 1300, 1329
BslI	CCNNNNNNNGG	9 cut(s)	75, 203, 347, 494, 887, 1145, 1296, 1331, 1353
BsmAI	GTCTC	3 cut(s)	9, 455, 1119
BsmFI	GGGAC	4 cut(s)	493, 1119, 1300, 1329
BsnI	GGCC	5 cut(s)	254, 263, 449, 626, 1176
Bso31I	GGTCTC	1 cut(s)	455
BsoBI	CYCGRG	1 cut(s)	257
Bsp1286I	GDGCHC	3 cut(s)	185, 702, 915
Bsp1407I	TGTACA	1 cut(s)	793
Bsp143I	GATC	8 cut(s)	167, 189, 217, 298, 318, 1090, 1141, 1249
BspACI	CCGC	6 cut(s)	51, 204, 243, 657, 758, 1297
BspANI	GGCC	5 cut(s)	254, 263, 449, 626, 1176
BspCNI	CTCAG	1 cut(s)	1018
BspFNI	CGCG	1 cut(s)	243
BspHI	TCATGA	1 cut(s)	354
BspLI	GGNNCC	4 cut(s)	208, 228, 1170, 1288
BspMI	ACCTGC	1 cut(s)	1007
BspOI	GCTAGC	1 cut(s)	339
BspPI	GGATC	3 cut(s)	162, 1085, 1244
BspT107I	GGYRCC	2 cut(s)	226, 1168
BspTNI	GGTCTC	1 cut(s)	455
BsrFI	RCCGGY	3 cut(s)	160, 831, 1313
BsrGI	TGTACA	1 cut(s)	793
BsrI	ACTGG	2 cut(s)	367, 650
BssAI	RCCGGY	3 cut(s)	160, 831, 1313
BssECI	CCNNGG	4 cut(s)	74, 210, 465, 915
BssMI	GATC	8 cut(s)	167, 189, 217, 298, 318, 1090, 1141, 1249
BssNI	GRCGYC	1 cut(s)	482
BssSI	CACGAG	2 cut(s)	125, 221
BssT1I	CCWWGG	3 cut(s)	210, 465, 915
Bst2BI	CACGAG	2 cut(s)	125, 221
Bst2UI	CCWGG	4 cut(s)	76, 603, 755, 1173
Bst4CI	ACNGT	1 cut(s)	1225
Bst6I	CTCTTC	4 cut(s)	615, 1115, 1280, 1389
BstACI	GRCGYC	1 cut(s)	482
BstAPI	GCANNNNNTGC	2 cut(s)	382, 770
BstAUI	TGTACA	1 cut(s)	793
BstBAI	YACGTR	1 cut(s)	1259
BstC8I	GCNNGC	9 cut(s)	162, 185, 239, 337, 383, 833, 1041, 1063, 1385
BstDEI	CTNAG	2 cut(s)	1026, 1428
BstENI	CCTNNNNNAGG	2 cut(s)	345, 885
BstF5I	GGATG	7 cut(s)	335, 877, 910, 925, 933, 961, 1358
BstFNI	CGCG	1 cut(s)	243
BstH2I	RGCGCY	1 cut(s)	754
BstHHI	GCGC	3 cut(s)	243, 753, 1067
BstKTI	GATC	8 cut(s)	170, 192, 220, 301, 321, 1093, 1144, 1252
BstMAI	GTCTC	3 cut(s)	9, 455, 1119
BstMBI	GATC	8 cut(s)	167, 189, 217, 298, 318, 1090, 1141, 1249
BstMWI	GCNNNNNNNGC	4 cut(s)	193, 260, 382, 770
BstNI	CCWGG	4 cut(s)	76, 603, 755, 1173
BstNSI	RCATGY	3 cut(s)	317, 430, 1043
BstSCI	CCNGG	5 cut(s)	74, 601, 753, 1171, 1345
BstSFI	CTRYAG	1 cut(s)	709
BstSLI	GKGCMC	1 cut(s)	915
BstUI	CGCG	1 cut(s)	243
BstV1I	GCAGC	4 cut(s)	258, 946, 1198, 1417
BstXI	CCANNNNNNTGG	1 cut(s)	445
BsuRI	GGCC	5 cut(s)	254, 263, 449, 626, 1176
BtgZI	GCGATG	1 cut(s)	1386
BtsCI	GGATG	7 cut(s)	335, 877, 910, 925, 933, 961, 1358
BtsIMutI	CAGTG	1 cut(s)	1221
BveI	ACCTGC	1 cut(s)	1007
Cac8I	GCNNGC	9 cut(s)	162, 185, 239, 337, 383, 833, 1041, 1063, 1385
CciI	TCATGA	1 cut(s)	354
CfoI	GCGC	3 cut(s)	243, 753, 1067
Cfr10I	RCCGGY	3 cut(s)	160, 831, 1313
Cfr13I	GGNCC	6 cut(s)	207, 253, 364, 506, 1019, 1287
CseI	GACGC	2 cut(s)	119, 770
Csp6I	GTAC	6 cut(s)	370, 613, 671, 794, 947, 1031
CviAII	CATG	8 cut(s)	155, 314, 355, 427, 806, 1040, 1145, 1165
CviQI	GTAC	6 cut(s)	370, 613, 671, 794, 947, 1031
DdeI	CTNAG	2 cut(s)	1026, 1428
DpnI	GATC	8 cut(s)	169, 191, 219, 300, 320, 1092, 1143, 1251
DpnII	GATC	8 cut(s)	167, 189, 217, 298, 318, 1090, 1141, 1249
DrdI	GACNNNNNNGTC	1 cut(s)	589
DriI	GACNNNNNGTC	1 cut(s)	205
DseDI	GACNNNNNNGTC	1 cut(s)	589
EaeI	YGGCCR	1 cut(s)	447
Eam1104I	CTCTTC	4 cut(s)	615, 1115, 1280, 1389
Eam1105I	GACNNNNNGTC	1 cut(s)	205
EarI	CTCTTC	4 cut(s)	615, 1115, 1280, 1389
EciI	GGCGGA	2 cut(s)	672, 773
Ecl136II	GAGCTC	1 cut(s)	183
Eco130I	CCWWGG	3 cut(s)	210, 465, 915
Eco24I	GRGCYC	2 cut(s)	185, 702
Eco31I	GGTCTC	1 cut(s)	455
Eco47I	GGWCC	5 cut(s)	207, 364, 506, 1019, 1287
Eco53kI	GAGCTC	1 cut(s)	183
Eco72I	CACGTG	1 cut(s)	1259
Eco88I	CYCGRG	1 cut(s)	257
EcoICRI	GAGCTC	1 cut(s)	183
EcoNI	CCTNNNNNAGG	2 cut(s)	345, 885
EcoO109I	RGGNCCY	2 cut(s)	207, 253
EcoRII	CCWGG	4 cut(s)	74, 601, 753, 1171
EcoT14I	CCWWGG	3 cut(s)	210, 465, 915
EcoT22I	ATGCAT	1 cut(s)	156
EcoT38I	GRGCYC	2 cut(s)	185, 702
ErhI	CCWWGG	3 cut(s)	210, 465, 915
FaeI	CATG	8 cut(s)	158, 317, 358, 430, 809, 1043, 1148, 1168
FalI	AAGNNNNNCTT	2 cut(s)	1112, 1144
FaqI	GGGAC	4 cut(s)	493, 1119, 1300, 1329
FatI	CATG	8 cut(s)	154, 313, 354, 426, 805, 1039, 1144, 1164
FauI	CCCGC	2 cut(s)	197, 236
FbaI	TGATCA	2 cut(s)	318, 1141
Fnu4HI	GCNGC	4 cut(s)	272, 935, 1212, 1406
FokI	GGATG	7 cut(s)	342, 864, 917, 932, 940, 968, 1365
FriOI	GRGCYC	2 cut(s)	185, 702
Fsp4HI	GCNGC	4 cut(s)	272, 935, 1212, 1406
FspBI	CTAG	4 cut(s)	336, 674, 950, 1364
GlaI	GCGC	3 cut(s)	242, 752, 1066
GluI	GCNGC	4 cut(s)	272, 935, 1212, 1406
GsuI	CTGGAG	2 cut(s)	323, 618
HaeII	RGCGCY	1 cut(s)	754
HaeIII	GGCC	5 cut(s)	254, 263, 449, 626, 1176
HapII	CCGG	7 cut(s)	161, 197, 230, 832, 902, 1314, 1347
HgaI	GACGC	2 cut(s)	119, 770
HhaI	GCGC	3 cut(s)	243, 753, 1067
Hin1I	GRCGYC	1 cut(s)	482
Hin1II	CATG	8 cut(s)	158, 317, 358, 430, 809, 1043, 1148, 1168
Hin6I	GCGC	3 cut(s)	241, 751, 1065
HinP1I	GCGC	3 cut(s)	241, 751, 1065
HincII	GTYRAC	2 cut(s)	424, 594
HindII	GTYRAC	2 cut(s)	424, 594
HindIII	AAGCTT	1 cut(s)	17
HinfI	GANTC	7 cut(s)	266, 402, 474, 714, 837, 859, 1397
HpaII	CCGG	7 cut(s)	161, 197, 230, 832, 902, 1314, 1347
HphI	GGTGA	2 cut(s)	40, 993
Hpy166II	GTNNAC	3 cut(s)	424, 594, 1369
Hpy188I	TCNGA	4 cut(s)	9, 109, 734, 927
Hpy188III	TCNNGA	6 cut(s)	178, 302, 355, 478, 965, 1328
Hpy8I	GTNNAC	3 cut(s)	424, 594, 1369
Hpy99I	CGWCG	2 cut(s)	461, 487
HpyAV	CCTTC	2 cut(s)	1263, 1351
HpyCH4III	ACNGT	1 cut(s)	1225
HpyCH4IV	ACGT	4 cut(s)	24, 482, 615, 1258
HpyF10VI	GCNNNNNNNGC	4 cut(s)	193, 260, 382, 770
HpyF3I	CTNAG	2 cut(s)	1026, 1428
HpySE526I	ACGT	4 cut(s)	24, 482, 615, 1258
Hsp92I	GRCGYC	1 cut(s)	482
Hsp92II	CATG	8 cut(s)	158, 317, 358, 430, 809, 1043, 1148, 1168
HspAI	GCGC	3 cut(s)	241, 751, 1065
KroI	GCCGGC	2 cut(s)	160, 831
KroNI	GCCGGC	2 cut(s)	162, 833
Ksp22I	TGATCA	2 cut(s)	318, 1141
Kzo9I	GATC	8 cut(s)	167, 189, 217, 298, 318, 1090, 1141, 1249
LmnI	GCTCC	3 cut(s)	246, 537, 931
Lsp1109I	GCAGC	4 cut(s)	258, 946, 1198, 1417
LweI	GCATC	1 cut(s)	1284
MaeI	CTAG	4 cut(s)	336, 674, 950, 1364
MaeII	ACGT	4 cut(s)	24, 482, 615, 1258
MaeIII	GTNAC	2 cut(s)	25, 851
MalI	GATC	8 cut(s)	169, 191, 219, 300, 320, 1092, 1143, 1251
MboI	GATC	8 cut(s)	167, 189, 217, 298, 318, 1090, 1141, 1249
MboII	GAAGA	7 cut(s)	578, 632, 1132, 1243, 1246, 1267, 1406
MhlI	GDGCHC	3 cut(s)	185, 702, 915
MlsI	TGGCCA	1 cut(s)	449
MluCI	AATT	4 cut(s)	391, 740, 864, 1069
MluNI	TGGCCA	1 cut(s)	449
MlyI	GAGTC	5 cut(s)	275, 411, 708, 831, 1406
MmeI	TCCRAC	2 cut(s)	484, 1046
Mox20I	TGGCCA	1 cut(s)	449
Mph1103I	ATGCAT	1 cut(s)	156
MreI	CGCCGGCG	1 cut(s)	831
MroNI	GCCGGC	2 cut(s)	160, 831
MscI	TGGCCA	1 cut(s)	449
MseI	TTAA	4 cut(s)	15, 80, 344, 533
MslI	CAYNNNNRTG	2 cut(s)	118, 149
Msp20I	TGGCCA	1 cut(s)	449
MspA1I	CMGCKG	1 cut(s)	638
MspI	CCGG	7 cut(s)	161, 197, 230, 832, 902, 1314, 1347
MspR9I	CCNGG	5 cut(s)	76, 603, 755, 1173, 1347
MvaI	CCWGG	4 cut(s)	76, 603, 755, 1173
MvnI	CGCG	1 cut(s)	243
MwoI	GCNNNNNNNGC	4 cut(s)	193, 260, 382, 770
NaeI	GCCGGC	2 cut(s)	162, 833
NciI	CCSGG	1 cut(s)	1347
NdeII	GATC	8 cut(s)	167, 189, 217, 298, 318, 1090, 1141, 1249
NgoMIV	GCCGGC	2 cut(s)	160, 831
NheI	GCTAGC	1 cut(s)	335
NlaIII	CATG	8 cut(s)	158, 317, 358, 430, 809, 1043, 1148, 1168
NlaIV	GGNNCC	4 cut(s)	208, 228, 1170, 1288
NmeAIII	GCCGAG	1 cut(s)	289
NsiI	ATGCAT	1 cut(s)	156
NspI	RCATGY	3 cut(s)	317, 430, 1043
PaeI	GCATGC	1 cut(s)	1043
PaeR7I	CTCGAG	1 cut(s)	257
PagI	TCATGA	1 cut(s)	354
PciI	ACATGT	1 cut(s)	426
PdiI	GCCGGC	2 cut(s)	162, 833
PfeI	GAWTC	2 cut(s)	474, 859
Pfl23II	CGTACG	1 cut(s)	612
PfoI	TCCNGGA	1 cut(s)	1345
PkrI	GCNGC	4 cut(s)	273, 936, 1213, 1407
PleI	GAGTC	5 cut(s)	274, 410, 708, 831, 1405
PmaCI	CACGTG	1 cut(s)	1259
PmlI	CACGTG	1 cut(s)	1259
PpsI	GAGTC	5 cut(s)	274, 410, 708, 831, 1405
Ppu21I	YACGTR	1 cut(s)	1259
PpuMI	RGGWCCY	1 cut(s)	207
PscI	ACATGT	1 cut(s)	426
Psp124BI	GAGCTC	1 cut(s)	185
Psp5II	RGGWCCY	1 cut(s)	207
Psp6I	CCWGG	4 cut(s)	74, 601, 753, 1171
PspCI	CACGTG	1 cut(s)	1259
PspGI	CCWGG	4 cut(s)	74, 601, 753, 1171
PspLI	CGTACG	1 cut(s)	612
PspN4I	GGNNCC	4 cut(s)	208, 228, 1170, 1288
PspPI	GGNCC	6 cut(s)	207, 253, 364, 506, 1019, 1287
PspPPI	RGGWCCY	1 cut(s)	207
PspXI	VCTCGAGB	1 cut(s)	257
PvuII	CAGCTG	1 cut(s)	638
RsaI	GTAC	6 cut(s)	371, 614, 672, 795, 948, 1032
RsaNI	GTAC	6 cut(s)	370, 613, 671, 794, 947, 1031
RseI	CAYNNNNRTG	2 cut(s)	118, 149
SacI	GAGCTC	1 cut(s)	185
SaqAI	TTAA	4 cut(s)	15, 80, 344, 533
SatI	GCNGC	4 cut(s)	272, 935, 1212, 1406
Sau3AI	GATC	8 cut(s)	167, 189, 217, 298, 318, 1090, 1141, 1249
Sau96I	GGNCC	6 cut(s)	207, 253, 364, 506, 1019, 1287
ScaI	AGTACT	2 cut(s)	371, 672
SchI	GAGTC	5 cut(s)	275, 411, 708, 831, 1406
ScrFI	CCNGG	5 cut(s)	76, 603, 755, 1173, 1347
SduI	GDGCHC	3 cut(s)	185, 702, 915
SfaNI	GCATC	1 cut(s)	1284
SfcI	CTRYAG	1 cut(s)	709
SfiI	GGCCNNNNNGGCC	1 cut(s)	260
Sfr274I	CTCGAG	1 cut(s)	257
SgrAI	CRCCGGYG	1 cut(s)	831
SinI	GGWCC	5 cut(s)	207, 364, 506, 1019, 1287
SlaI	CTCGAG	1 cut(s)	257
SmiMI	CAYNNNNRTG	2 cut(s)	118, 149
SmlI	CTYRAG	2 cut(s)	257, 1178
SmoI	CTYRAG	2 cut(s)	257, 1178
SphI	GCATGC	1 cut(s)	1043
Sse9I	AATT	4 cut(s)	391, 740, 864, 1069
SsiI	CCGC	6 cut(s)	51, 204, 243, 657, 758, 1297
SspI	AATATT	1 cut(s)	1192
SspMI	CTAG	4 cut(s)	336, 674, 950, 1364
SstI	GAGCTC	1 cut(s)	185
StyD4I	CCNGG	5 cut(s)	74, 601, 753, 1171, 1345
StyI	CCWWGG	3 cut(s)	210, 465, 915
TaaI	ACNGT	1 cut(s)	1225
TaiI	ACGT	4 cut(s)	27, 485, 618, 1261
TaqI	TCGA	6 cut(s)	166, 192, 216, 258, 702, 966
TasI	AATT	4 cut(s)	391, 740, 864, 1069
TatI	WGTACW	3 cut(s)	369, 670, 793
TfiI	GAWTC	2 cut(s)	474, 859
Tru1I	TTAA	4 cut(s)	15, 80, 344, 533
Tru9I	TTAA	4 cut(s)	15, 80, 344, 533
TscAI	CASTG	1 cut(s)	1228
TseI	GCWGC	4 cut(s)	271, 934, 1211, 1405
TspDTI	ATGAA	4 cut(s)	343, 833, 866, 1267
TspGWI	ACGGA	1 cut(s)	1066
TspRI	CASTG	1 cut(s)	1228
VpaK11BI	GGWCC	5 cut(s)	207, 364, 506, 1019, 1287
XagI	CCTNNNNNAGG	2 cut(s)	345, 885
XapI	RAATTY	2 cut(s)	740, 1069
XceI	RCATGY	3 cut(s)	317, 430, 1043
XcmI	CCANNNNNNNNNTGG	2 cut(s)	298, 642
XhoI	CTCGAG	1 cut(s)	257
XspI	CTAG	4 cut(s)	336, 674, 950, 1364
ZraI	GACGTC	1 cut(s)	483
ZrmI	AGTACT	2 cut(s)	371, 672
Zsp2I	ATGCAT	1 cut(s)	156

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.