Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000007

Orthogroup ID

OG0013918

Physical Location & Seq

Genomic Coordinates Forward (+)

7230628 .. 7233744

Gene Structure 3117 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_7G00165530.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1299 bp

ATGTCTCTGACTATAAAGCTTCACGTCACCACCTCCCACCTCAACCTCAACCTCAACATCACCGCAATCAAAAGACCAACCCTCCCCAGGTTAAGAACCACCAAACCCATTTACCATTCTGACTCATTATTGTCCCTCGTGTATAAACGGAAACCCACAACAATGCATGAGCCGGCTCGATCCACCATCAATAGCTCGCTGATCGAGCCAGACGGCGGGGCCTTGGTCGATCTCGTGGTGCCGGAAAGCGACCGTGGGACCAAGGCCCTCGAGGCCGAGTCGCTGCCGAAGGTGAGCCTGACCAAGATTGATCTGGAGTGGGTGCATGTGATCAGCGAGGGATGGGCTAGCCCTTTAAGAGGGTTCATGAGGGAGGACCAGTACTTGCAGAGCTTGCACTTCAATTCGTTGAGAGTCAAGGACGGGTCTGTGGTCAACATGTCGCTTCCCATTGTGTTGGCCATTGACGACGAGACCAAGGAGAGAATCGGGACGTCGCCGAATGTGGGGTTGGTTGGACCTAATGGAGATTTGATTGGGATATTAAGGAGCATTGAGATATACAAGCATAACAAAGAAGAAAGGATTGCGAGGACTTGGGGGTCAACTGCACCAGGATTGCCGTACGTTGAGGAGGCCATCACTCCAGCTGGAAACTGGCTTATTGGCGGAGATTTAGAAGTACTAGAGCATATCAAATACAATGATGGGCTCGATAACTACAGACTCTCTCCTCAACAACTCCGAAACGAATTTGATAGGCGCCAGGCGGACGCAGTTTTTGCGTTTCAGTTGAGAAATCCTGTACATAATGGTCATGCTCTGTTGATGAATGATACACGCCAGCGACTCTTGGAAATGGGTTACAAGAATCCAATTCTATTGCTTCATCCTTTAGGAGGTTTCACAAAGCCGGATGATGTGCCCTTGGATGTTCGGATGGAGCAACATAGCAAGGTACTAGAGGATGGAGTTCTTGACCCTGAAACTACCATCGTGGGCATATTCCCATCACCTATGCACTATGCAGGTCCAACTGAGGTGGCTGCTTATGACACTGTGGAGAAGAAGATGGCATTTTTTGATCCTTCACGTGCAACAGATTTCCTCTTCATCTCTGGGACCAAGGTAGGAACTGAATTTCAATTCAATGTCAGTATTATGCGGACTTATGCAAAGACCGGCGAAAACCCTCCTGATGGTTTTATGTGTCCCGGAGGATGGAAGGTTCTAGTGAACTACTACGAGAGCTTGCAAACAGAAGAGTCATCGCAGCAGCAAGCTGTTTTATCTACTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000103 GO:0003674 GO:0003824 GO:0004020 GO:0004779 GO:0004781 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006790 GO:0006793 GO:0006796 GO:0006950 GO:0007154 GO:0008150 GO:0008152 GO:0009267 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009970 GO:0009987 GO:0009991 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016779 GO:0031667 GO:0031668 GO:0031669 GO:0033554 GO:0042594 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0050896 GO:0051716 GO:0070566 GO:0071496

KEGG Pathways

Metabolic & Signaling

ko00230 ko00261 ko00450 ko00920 ko01100 ko01120 ko01130 map00230 map00261 map00450 map00920 map01100 map01120 map01130

Pfam Domains

Protein Families

ATP-sulfurylase PUA_2

Protein Analysis

432

Amino Acids

48.28

Weight (kDa)

5.75

Isoelectric Point (pI)

50.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PUA_2	PF14306	68 - 230	1.3e-42	PUA-like domain
ATP-sulfurylase	PF01747	240 - 349	1.7e-32	ATP-sulfurylase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013918)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G19920
fragaria_vesca	FvH4_2g38710
malus_domestica	MD08G1014800.v1.1 MD15G1014100.v1.1
prunus_persica	Prupe.1G367700_v2.0.a1
pyrus_communis	pycom08g01320 pycom15g01220
rosa_chinensis	RchiOBHm_Chr6g0302601
rosa_laevigata	RLG00000011158
rosa_multiflora	Rmu_sc0005455.1_g000010
rosa_roxburghii	Rroxscaffold_7G00165530
rosa_rugosa	Rorug06G0315200
rosa_samantha	Rh6AG428600 Rh6BG473900 Rh6CG440800 Rh6DG427600
rosa_wichuraiana	Rw6G037090

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	601
AatII	GACGTC	1 cut(s)	497
AbsI	CCTCGAGG	1 cut(s)	269
Acc36I	ACCTGC	1 cut(s)	1019
AccB1I	GGYRCC	2 cut(s)	238, 762
AciI	CCGC	5 cut(s)	63, 216, 669, 770, 1165
AclWI	GGATC	2 cut(s)	174, 1079
AcoI	YGGCCR	1 cut(s)	459
AcsI	RAATTY	2 cut(s)	752, 1139
AcvI	CACGTG	1 cut(s)	1094
AcyI	GRCGYC	2 cut(s)	494, 763
AfaI	GTAC	5 cut(s)	383, 626, 684, 807, 960
AfiI	CCNNNNNNNGG	7 cut(s)	87, 215, 359, 506, 899, 1199, 1221
AflIII	ACRYGT	1 cut(s)	438
AgsI	TTSAA	3 cut(s)	403, 1145, 1150
AjiI	CACGTC	1 cut(s)	25
AjnI	CCWGG	3 cut(s)	86, 613, 765
AjuI	GAANNNNNNNTTGG	2 cut(s)	494, 526
AloI	GAACNNNNNNTCC	2 cut(s)	1212, 1244
AluBI	AGCT	6 cut(s)	19, 195, 393, 650, 1251, 1283
AluI	AGCT	6 cut(s)	19, 195, 393, 650, 1251, 1283
Alw26I	GTCTC	2 cut(s)	9, 467
AlwI	GGATC	2 cut(s)	174, 1079
Ama87I	CYCGRG	1 cut(s)	269
AoxI	GGCC	5 cut(s)	219, 264, 273, 459, 636
ApeKI	GCWGC	4 cut(s)	283, 1046, 1273, 1276
ApoI	RAATTY	2 cut(s)	752, 1139
ArsI	GACNNNNNNTTYG	2 cut(s)	764, 796
AspLEI	GCGC	1 cut(s)	765
AspS9I	GGNCC	7 cut(s)	219, 258, 265, 376, 518, 1031, 1122
AsuC2I	CCSGG	1 cut(s)	1215
AsuHPI	GGTGA	4 cut(s)	19, 52, 304, 1005
AsuNHI	GCTAGC	1 cut(s)	347
AvaI	CYCGRG	1 cut(s)	269
AvaII	GGWCC	5 cut(s)	258, 376, 518, 1031, 1122
BaeGI	GKGCMC	1 cut(s)	927
BalI	TGGCCA	1 cut(s)	461
BanI	GGYRCC	2 cut(s)	238, 762
BanII	GRGCYC	1 cut(s)	714
BauI	CACGAG	2 cut(s)	137, 233
BbrPI	CACGTG	1 cut(s)	1094
BbvI	GCAGC	4 cut(s)	270, 1033, 1285, 1288
BceAI	ACGGC	2 cut(s)	229, 607
BciT130I	CCWGG	3 cut(s)	88, 615, 767
BclI	TGATCA	1 cut(s)	330
BcnI	CCSGG	1 cut(s)	1215
BcoDI	GTCTC	2 cut(s)	9, 467
BfaI	CTAG	4 cut(s)	348, 686, 962, 1232
BfmI	CTRYAG	1 cut(s)	721
BfoI	RGCGCY	1 cut(s)	766
BfuAI	ACCTGC	1 cut(s)	1019
BglI	GCCNNNNNGGC	1 cut(s)	272
BisI	GCNGC	4 cut(s)	284, 1047, 1274, 1277
BlsI	GCNGC	4 cut(s)	285, 1048, 1275, 1278
BmcAI	AGTACT	2 cut(s)	383, 684
Bme1390I	CCNGG	4 cut(s)	88, 615, 767, 1215
Bme18I	GGWCC	5 cut(s)	258, 376, 518, 1031, 1122
BmeT110I	CYCGRG	1 cut(s)	269
BmgBI	CACGTC	1 cut(s)	25
BmgT120I	GGNCC	7 cut(s)	219, 258, 265, 376, 518, 1031, 1122
BmiI	GGNNCC	5 cut(s)	220, 240, 259, 764, 1123
BmrFI	CCNGG	4 cut(s)	88, 615, 767, 1215
BmtI	GCTAGC	1 cut(s)	351
BpmI	CTGGAG	2 cut(s)	335, 630
BpuMI	CCSGG	1 cut(s)	1215
BsaAI	YACGTR	1 cut(s)	1094
BsaHI	GRCGYC	2 cut(s)	494, 763
BsaI	GGTCTC	1 cut(s)	467
BsaJI	CCNNGG	7 cut(s)	86, 222, 253, 261, 477, 927, 1125
BsaXI	ACNNNNNCTCC	4 cut(s)	66, 96, 365, 395
Bsc4I	CCNNNNNNNGG	7 cut(s)	87, 215, 359, 506, 899, 1199, 1221
Bse118I	RCCGGY	2 cut(s)	172, 1181
Bse1I	ACTGG	2 cut(s)	379, 662
BseBI	CCWGG	3 cut(s)	88, 615, 767
BseDI	CCNNGG	7 cut(s)	86, 222, 253, 261, 477, 927, 1125
BseGI	GGATG	7 cut(s)	347, 889, 922, 937, 945, 973, 1226
BseLI	CCNNNNNNNGG	7 cut(s)	87, 215, 359, 506, 899, 1199, 1221
BseMII	CTCAG	1 cut(s)	1029
BseNI	ACTGG	2 cut(s)	379, 662
BseRI	GAGGAG	2 cut(s)	647, 723
BseSI	GKGCMC	1 cut(s)	927
BseXI	GCAGC	4 cut(s)	270, 1033, 1285, 1288
BsgI	GTGCAG	1 cut(s)	594
Bsh1285I	CGRYCG	1 cut(s)	253
BshFI	GGCC	5 cut(s)	221, 266, 275, 461, 638
BshNI	GGYRCC	2 cut(s)	238, 762
BsiEI	CGRYCG	1 cut(s)	253
BsiHKCI	CYCGRG	1 cut(s)	269
BsiSI	CCGG	5 cut(s)	173, 242, 914, 1182, 1215
BsiWI	CGTACG	1 cut(s)	624
BslFI	GGGAC	5 cut(s)	118, 271, 505, 1135, 1197
BslI	CCNNNNNNNGG	7 cut(s)	87, 215, 359, 506, 899, 1199, 1221
BsmAI	GTCTC	2 cut(s)	9, 467
BsmFI	GGGAC	5 cut(s)	118, 271, 505, 1135, 1197
BsnI	GGCC	5 cut(s)	221, 266, 275, 461, 638
Bso31I	GGTCTC	1 cut(s)	467
BsoBI	CYCGRG	1 cut(s)	269
Bsp1286I	GDGCHC	2 cut(s)	714, 927
Bsp1407I	TGTACA	1 cut(s)	805
Bsp143I	GATC	6 cut(s)	179, 201, 229, 310, 330, 1084
BspACI	CCGC	5 cut(s)	63, 216, 669, 770, 1165
BspANI	GGCC	5 cut(s)	221, 266, 275, 461, 638
BspCNI	CTCAG	1 cut(s)	1030
BspHI	TCATGA	1 cut(s)	366
BspLI	GGNNCC	5 cut(s)	220, 240, 259, 764, 1123
BspMI	ACCTGC	1 cut(s)	1019
BspOI	GCTAGC	1 cut(s)	351
BspPI	GGATC	2 cut(s)	174, 1079
BspT107I	GGYRCC	2 cut(s)	238, 762
BspTNI	GGTCTC	1 cut(s)	467
BsrFI	RCCGGY	2 cut(s)	172, 1181
BsrGI	TGTACA	1 cut(s)	805
BsrI	ACTGG	2 cut(s)	379, 662
BssAI	RCCGGY	2 cut(s)	172, 1181
BssECI	CCNNGG	7 cut(s)	86, 222, 253, 261, 477, 927, 1125
BssMI	GATC	6 cut(s)	179, 201, 229, 310, 330, 1084
BssNI	GRCGYC	2 cut(s)	494, 763
BssSI	CACGAG	2 cut(s)	137, 233
BssT1I	CCWWGG	5 cut(s)	222, 261, 477, 927, 1125
Bst2BI	CACGAG	2 cut(s)	137, 233
Bst2UI	CCWGG	3 cut(s)	88, 615, 767
Bst4CI	ACNGT	2 cut(s)	254, 1060
Bst6I	CTCTTC	2 cut(s)	1115, 1257
BstACI	GRCGYC	2 cut(s)	494, 763
BstAPI	GCANNNNNTGC	2 cut(s)	394, 782
BstAUI	TGTACA	1 cut(s)	805
BstBAI	YACGTR	1 cut(s)	1094
BstC8I	GCNNGC	7 cut(s)	174, 197, 349, 395, 845, 1253, 1281
BstDEI	CTNAG	2 cut(s)	1038, 1296
BstDSI	CCRYGG	1 cut(s)	253
BstENI	CCTNNNNNAGG	2 cut(s)	357, 897
BstF5I	GGATG	7 cut(s)	347, 889, 922, 937, 945, 973, 1226
BstH2I	RGCGCY	1 cut(s)	766
BstHHI	GCGC	1 cut(s)	765
BstKTI	GATC	6 cut(s)	182, 204, 232, 313, 333, 1087
BstMAI	GTCTC	2 cut(s)	9, 467
BstMBI	GATC	6 cut(s)	179, 201, 229, 310, 330, 1084
BstMCI	CGRYCG	1 cut(s)	253
BstMWI	GCNNNNNNNGC	4 cut(s)	205, 272, 394, 782
BstNI	CCWGG	3 cut(s)	88, 615, 767
BstNSI	RCATGY	2 cut(s)	329, 442
BstSCI	CCNGG	4 cut(s)	86, 613, 765, 1213
BstSFI	CTRYAG	1 cut(s)	721
BstSLI	GKGCMC	1 cut(s)	927
BstV1I	GCAGC	4 cut(s)	270, 1033, 1285, 1288
BstXI	CCANNNNNNTGG	1 cut(s)	457
BsuRI	GGCC	5 cut(s)	221, 266, 275, 461, 638
BtgI	CCRYGG	1 cut(s)	253
BtgZI	GCGATG	1 cut(s)	1254
BtrI	CACGTC	1 cut(s)	25
BtsCI	GGATG	7 cut(s)	347, 889, 922, 937, 945, 973, 1226
BtsIMutI	CAGTG	1 cut(s)	1056
BveI	ACCTGC	1 cut(s)	1019
Cac8I	GCNNGC	7 cut(s)	174, 197, 349, 395, 845, 1253, 1281
CciI	TCATGA	1 cut(s)	366
CfoI	GCGC	1 cut(s)	765
Cfr10I	RCCGGY	2 cut(s)	172, 1181
Cfr13I	GGNCC	7 cut(s)	219, 258, 265, 376, 518, 1031, 1122
CseI	GACGC	1 cut(s)	782
Csp6I	GTAC	5 cut(s)	382, 625, 683, 806, 959
CspCI	CAANNNNNGTGG	2 cut(s)	1023, 1058
CviAII	CATG	5 cut(s)	167, 326, 367, 439, 818
CviQI	GTAC	5 cut(s)	382, 625, 683, 806, 959
DdeI	CTNAG	2 cut(s)	1038, 1296
DinI	GGCGCC	1 cut(s)	764
DpnI	GATC	6 cut(s)	181, 203, 231, 312, 332, 1086
DpnII	GATC	6 cut(s)	179, 201, 229, 310, 330, 1084
DrdI	GACNNNNNNGTC	1 cut(s)	601
DseDI	GACNNNNNNGTC	1 cut(s)	601
EaeI	YGGCCR	1 cut(s)	459
Eam1104I	CTCTTC	2 cut(s)	1115, 1257
EarI	CTCTTC	2 cut(s)	1115, 1257
EciI	GGCGGA	2 cut(s)	684, 785
Eco130I	CCWWGG	5 cut(s)	222, 261, 477, 927, 1125
Eco24I	GRGCYC	1 cut(s)	714
Eco31I	GGTCTC	1 cut(s)	467
Eco47I	GGWCC	5 cut(s)	258, 376, 518, 1031, 1122
Eco72I	CACGTG	1 cut(s)	1094
Eco88I	CYCGRG	1 cut(s)	269
EcoNI	CCTNNNNNAGG	2 cut(s)	357, 897
EcoO109I	RGGNCCY	2 cut(s)	219, 265
EcoRII	CCWGG	3 cut(s)	86, 613, 765
EcoT14I	CCWWGG	5 cut(s)	222, 261, 477, 927, 1125
EcoT22I	ATGCAT	1 cut(s)	168
EcoT38I	GRGCYC	1 cut(s)	714
EgeI	GGCGCC	1 cut(s)	764
EheI	GGCGCC	1 cut(s)	764
ErhI	CCWWGG	5 cut(s)	222, 261, 477, 927, 1125
FaeI	CATG	5 cut(s)	170, 329, 370, 442, 821
FaqI	GGGAC	5 cut(s)	118, 271, 505, 1135, 1197
FatI	CATG	5 cut(s)	166, 325, 366, 438, 817
FauI	CCCGC	1 cut(s)	209
FbaI	TGATCA	1 cut(s)	330
Fnu4HI	GCNGC	4 cut(s)	284, 1047, 1274, 1277
FokI	GGATG	7 cut(s)	354, 876, 929, 944, 952, 980, 1233
FriOI	GRGCYC	1 cut(s)	714
Fsp4HI	GCNGC	4 cut(s)	284, 1047, 1274, 1277
FspBI	CTAG	4 cut(s)	348, 686, 962, 1232
GlaI	GCGC	1 cut(s)	764
GluI	GCNGC	4 cut(s)	284, 1047, 1274, 1277
GsuI	CTGGAG	2 cut(s)	335, 630
HaeII	RGCGCY	1 cut(s)	766
HaeIII	GGCC	5 cut(s)	221, 266, 275, 461, 638
HapII	CCGG	5 cut(s)	173, 242, 914, 1182, 1215
HgaI	GACGC	1 cut(s)	782
HhaI	GCGC	1 cut(s)	765
Hin1I	GRCGYC	2 cut(s)	494, 763
Hin1II	CATG	5 cut(s)	170, 329, 370, 442, 821
Hin6I	GCGC	1 cut(s)	763
HinP1I	GCGC	1 cut(s)	763
HincII	GTYRAC	2 cut(s)	436, 606
HindII	GTYRAC	2 cut(s)	436, 606
HindIII	AAGCTT	1 cut(s)	17
HinfI	GANTC	8 cut(s)	122, 278, 414, 486, 726, 849, 871, 1265
HpaII	CCGG	5 cut(s)	173, 242, 914, 1182, 1215
HphI	GGTGA	4 cut(s)	19, 52, 304, 1005
Hpy166II	GTNNAC	3 cut(s)	436, 606, 1237
Hpy188I	TCNGA	4 cut(s)	9, 121, 746, 939
Hpy188III	TCNNGA	5 cut(s)	314, 367, 490, 977, 1196
Hpy8I	GTNNAC	3 cut(s)	436, 606, 1237
Hpy99I	CGWCG	2 cut(s)	473, 499
HpyAV	CCTTC	3 cut(s)	283, 1098, 1219
HpyCH4III	ACNGT	2 cut(s)	254, 1060
HpyCH4IV	ACGT	4 cut(s)	24, 494, 627, 1093
HpyF10VI	GCNNNNNNNGC	4 cut(s)	205, 272, 394, 782
HpyF3I	CTNAG	2 cut(s)	1038, 1296
HpySE526I	ACGT	4 cut(s)	24, 494, 627, 1093
Hsp92I	GRCGYC	2 cut(s)	494, 763
Hsp92II	CATG	5 cut(s)	170, 329, 370, 442, 821
HspAI	GCGC	1 cut(s)	763
KasI	GGCGCC	1 cut(s)	762
KroI	GCCGGC	1 cut(s)	172
KroNI	GCCGGC	1 cut(s)	174
Ksp22I	TGATCA	1 cut(s)	330
Kzo9I	GATC	6 cut(s)	179, 201, 229, 310, 330, 1084
LmnI	GCTCC	2 cut(s)	549, 943
Lsp1109I	GCAGC	4 cut(s)	270, 1033, 1285, 1288
MaeI	CTAG	4 cut(s)	348, 686, 962, 1232
MaeII	ACGT	4 cut(s)	24, 494, 627, 1093
MaeIII	GTNAC	2 cut(s)	25, 863
MalI	GATC	6 cut(s)	181, 203, 231, 312, 332, 1086
MboI	GATC	6 cut(s)	179, 201, 229, 310, 330, 1084
MboII	GAAGA	5 cut(s)	590, 1078, 1081, 1102, 1274
MhlI	GDGCHC	2 cut(s)	714, 927
MlsI	TGGCCA	1 cut(s)	461
MluCI	AATT	5 cut(s)	403, 752, 876, 1139, 1145
MluNI	TGGCCA	1 cut(s)	461
Mly113I	GGCGCC	1 cut(s)	763
MlyI	GAGTC	6 cut(s)	116, 287, 423, 720, 843, 1274
MmeI	TCCRAC	2 cut(s)	496, 1058
Mox20I	TGGCCA	1 cut(s)	461
Mph1103I	ATGCAT	1 cut(s)	168
MroNI	GCCGGC	1 cut(s)	172
MscI	TGGCCA	1 cut(s)	461
MseI	TTAA	3 cut(s)	92, 356, 545
MslI	CAYNNNNRTG	1 cut(s)	161
Msp20I	TGGCCA	1 cut(s)	461
MspA1I	CMGCKG	1 cut(s)	650
MspI	CCGG	5 cut(s)	173, 242, 914, 1182, 1215
MspR9I	CCNGG	4 cut(s)	88, 615, 767, 1215
MvaI	CCWGG	3 cut(s)	88, 615, 767
MwoI	GCNNNNNNNGC	4 cut(s)	205, 272, 394, 782
NaeI	GCCGGC	1 cut(s)	174
NarI	GGCGCC	1 cut(s)	763
NciI	CCSGG	1 cut(s)	1215
NdeII	GATC	6 cut(s)	179, 201, 229, 310, 330, 1084
NgoMIV	GCCGGC	1 cut(s)	172
NheI	GCTAGC	1 cut(s)	347
NlaIII	CATG	5 cut(s)	170, 329, 370, 442, 821
NlaIV	GGNNCC	5 cut(s)	220, 240, 259, 764, 1123
NmeAIII	GCCGAG	1 cut(s)	301
NmuCI	GTSAC	1 cut(s)	25
NsiI	ATGCAT	1 cut(s)	168
NspI	RCATGY	2 cut(s)	329, 442
PaeR7I	CTCGAG	1 cut(s)	269
PagI	TCATGA	1 cut(s)	366
PciI	ACATGT	1 cut(s)	438
PdiI	GCCGGC	1 cut(s)	174
PfeI	GAWTC	2 cut(s)	486, 871
Pfl23II	CGTACG	1 cut(s)	624
PfoI	TCCNGGA	1 cut(s)	1213
PkrI	GCNGC	4 cut(s)	285, 1048, 1275, 1278
PleI	GAGTC	6 cut(s)	116, 286, 422, 720, 843, 1273
PluTI	GGCGCC	1 cut(s)	766
PmaCI	CACGTG	1 cut(s)	1094
PmlI	CACGTG	1 cut(s)	1094
PpsI	GAGTC	6 cut(s)	116, 286, 422, 720, 843, 1273
Ppu21I	YACGTR	1 cut(s)	1094
PscI	ACATGT	1 cut(s)	438
Psp6I	CCWGG	3 cut(s)	86, 613, 765
PspCI	CACGTG	1 cut(s)	1094
PspGI	CCWGG	3 cut(s)	86, 613, 765
PspLI	CGTACG	1 cut(s)	624
PspN4I	GGNNCC	5 cut(s)	220, 240, 259, 764, 1123
PspPI	GGNCC	7 cut(s)	219, 258, 265, 376, 518, 1031, 1122
PspXI	VCTCGAGB	1 cut(s)	269
PvuII	CAGCTG	1 cut(s)	650
RsaI	GTAC	5 cut(s)	383, 626, 684, 807, 960
RsaNI	GTAC	5 cut(s)	382, 625, 683, 806, 959
RseI	CAYNNNNRTG	1 cut(s)	161
SaqAI	TTAA	3 cut(s)	92, 356, 545
SatI	GCNGC	4 cut(s)	284, 1047, 1274, 1277
Sau3AI	GATC	6 cut(s)	179, 201, 229, 310, 330, 1084
Sau96I	GGNCC	7 cut(s)	219, 258, 265, 376, 518, 1031, 1122
ScaI	AGTACT	2 cut(s)	383, 684
SchI	GAGTC	6 cut(s)	116, 287, 423, 720, 843, 1274
ScrFI	CCNGG	4 cut(s)	88, 615, 767, 1215
SduI	GDGCHC	2 cut(s)	714, 927
SfcI	CTRYAG	1 cut(s)	721
SfiI	GGCCNNNNNGGCC	1 cut(s)	272
SfoI	GGCGCC	1 cut(s)	764
Sfr274I	CTCGAG	1 cut(s)	269
SinI	GGWCC	5 cut(s)	258, 376, 518, 1031, 1122
SlaI	CTCGAG	1 cut(s)	269
SmiMI	CAYNNNNRTG	1 cut(s)	161
SmlI	CTYRAG	1 cut(s)	269
SmoI	CTYRAG	1 cut(s)	269
Sse9I	AATT	5 cut(s)	403, 752, 876, 1139, 1145
SsiI	CCGC	5 cut(s)	63, 216, 669, 770, 1165
SspDI	GGCGCC	1 cut(s)	762
SspMI	CTAG	4 cut(s)	348, 686, 962, 1232
StyD4I	CCNGG	4 cut(s)	86, 613, 765, 1213
StyI	CCWWGG	5 cut(s)	222, 261, 477, 927, 1125
TaaI	ACNGT	2 cut(s)	254, 1060
TaiI	ACGT	4 cut(s)	27, 497, 630, 1096
TaqI	TCGA	5 cut(s)	178, 204, 228, 270, 714
TasI	AATT	5 cut(s)	403, 752, 876, 1139, 1145
TatI	WGTACW	3 cut(s)	381, 682, 805
TfiI	GAWTC	2 cut(s)	486, 871
Tru1I	TTAA	3 cut(s)	92, 356, 545
Tru9I	TTAA	3 cut(s)	92, 356, 545
TscAI	CASTG	1 cut(s)	1063
TseFI	GTSAC	1 cut(s)	25
TseI	GCWGC	4 cut(s)	283, 1046, 1273, 1276
Tsp45I	GTSAC	1 cut(s)	25
TspDTI	ATGAA	4 cut(s)	355, 845, 878, 1102
TspGWI	ACGGA	1 cut(s)	163
TspRI	CASTG	1 cut(s)	1063
VpaK11BI	GGWCC	5 cut(s)	258, 376, 518, 1031, 1122
XagI	CCTNNNNNAGG	2 cut(s)	357, 897
XapI	RAATTY	2 cut(s)	752, 1139
XceI	RCATGY	2 cut(s)	329, 442
XcmI	CCANNNNNNNNNTGG	2 cut(s)	310, 654
XhoI	CTCGAG	1 cut(s)	269
XspI	CTAG	4 cut(s)	348, 686, 962, 1232
ZraI	GACGTC	1 cut(s)	495
ZrmI	AGTACT	2 cut(s)	383, 684
Zsp2I	ATGCAT	1 cut(s)	168

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.