MD15G1044600.v1.1

AarF domain-containing protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0018490
Physical Location & Seq
Forward (+)
3059999 .. 3061760
1762 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1044600.v1.1.491

Sequence Viewer

Length: 684 bp
ATGAGGAATGTCTTTGCCGCCCTTAAGCGCCACCGCCACGTCACTCTGCTCTCCCAAACTCTACACCATGAGAGAAAATGCACTCCATTGGGATGCCATAAATATGTTGAAATTAGATGGAGACAAGAAAATTGGTTTCCATGTCAATTGTTACAGCAACATTCTATTTCCACCAGCGTGCATGGTGAAATGCCATCGGCAGAGTATGCAAAGTTGAGGAAGGAATCACTAGAAAGTGAATTCGGGCATGCTCTCGATGCAGGCTCCAAAAGTATTTCTATTGTCTACTGCTTTGGTCCGTTTTTAGCTTTGTATAGAGCTGCAATCATTTCATTTCATGTGGTGAAGCTCACAGTCTGGCAATTCTTTGTCCTTGACATAAGGAAACGCGCCGTCAAGTTTCGTGAAACTCTTATCCGCTTGGGACCTTTCTACATCAAGCTCGGGCAGGCTTTGAGCACCAGGCGAGACATACTAGCAACTATATACTGCCAAGAACTTGTTAAGTTACAGGATCAAATACCTCCATTTCCAACGTGTATTGCAATTAAATCTATTGAATCTCAGCTGGGTGCCCCCATTTCAAAACTTTTTGCTGACATCAGCACAGAGCCTGTTGCAGCAGCATCCCTAGGGCAGGCCTATAAAGTTTTCCAGCTCACTTGCACTCTGGGGAGCTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC1

Protein Analysis

228

Amino Acids

25.86

Weight (kDa)

9.47

Isoelectric Point (pI)

46.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC1 PF03109 169 - 216 1.3e-09 ABC1 atypical kinase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0018490)

Species Orthologous Gene IDs
malus_domestica MD15G1044600.v1.1
rosa_chinensis RchiOBHm_Chr4g0400831
rosa_laevigata RLG00000022624
rosa_multiflora Rmu_co8395409.1_g000001 Rmu_sc0002701.1_g000020
rosa_roxburghii Rroxscaffold_1G00024720
rosa_rugosa Rorug01G0183700 Rorug04G0090900 Rorug05G0207800
rosa_samantha Rh6AG235800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 572
AccI GTMKAC 1 cut(s) 285
AccII CGCG 1 cut(s) 390
AciI CCGC 3 cut(s) 18, 34, 418
AclWI GGATC 1 cut(s) 522
AcsI RAATTY 1 cut(s) 239
AfiI CCNNNNNNNGG 1 cut(s) 637
AflII CTTAAG 1 cut(s) 23
AflIII ACRYGT 1 cut(s) 536
AgsI TTSAA 3 cut(s) 110, 560, 585
AjiI CACGTC 1 cut(s) 40
AjnI CCWGG 1 cut(s) 461
AleI CACNNNNGTG 1 cut(s) 176
AluBI AGCT 7 cut(s) 308, 320, 349, 442, 568, 658, 678
AluI AGCT 7 cut(s) 308, 320, 349, 442, 568, 658, 678
Alw21I GWGCWC 1 cut(s) 461
Alw26I GTCTC 2 cut(s) 115, 462
AlwI GGATC 1 cut(s) 522
AlwNI CAGNNNCTG 1 cut(s) 614
Ama87I CYCGRG 1 cut(s) 443
AoxI GGCC 1 cut(s) 639
ApeKI GCWGC 3 cut(s) 320, 620, 623
ApoI RAATTY 1 cut(s) 239
AspA2I CCTAGG 1 cut(s) 631
AspLEI GCGC 2 cut(s) 30, 392
AspS9I GGNCC 2 cut(s) 296, 425
AsuHPI GGTGA 2 cut(s) 197, 355
AvaI CYCGRG 1 cut(s) 443
AvaII GGWCC 2 cut(s) 296, 425
AvrII CCTAGG 1 cut(s) 631
BaeGI GKGCMC 1 cut(s) 577
BanI GGYRCC 1 cut(s) 572
Bbv12I GWGCWC 1 cut(s) 461
BbvI GCAGC 3 cut(s) 307, 632, 635
BccI CCATC 2 cut(s) 111, 202
BceAI ACGGC 1 cut(s) 377
BciT130I CCWGG 1 cut(s) 463
BcoDI GTCTC 2 cut(s) 115, 462
BfaI CTAG 3 cut(s) 230, 476, 632
BfoI RGCGCY 1 cut(s) 31
BfrI CTTAAG 1 cut(s) 23
BisI GCNGC 4 cut(s) 18, 321, 621, 624
BlnI CCTAGG 1 cut(s) 631
BlsI GCNGC 4 cut(s) 19, 322, 622, 625
Bme1390I CCNGG 1 cut(s) 463
Bme18I GGWCC 2 cut(s) 296, 425
BmeT110I CYCGRG 1 cut(s) 443
BmgBI CACGTC 1 cut(s) 40
BmgT120I GGNCC 2 cut(s) 296, 425
BmiI GGNNCC 3 cut(s) 265, 426, 574
BmrFI CCNGG 1 cut(s) 463
BmsI GCATC 3 cut(s) 83, 247, 635
BsaJI CCNNGG 1 cut(s) 631
Bsc4I CCNNNNNNNGG 1 cut(s) 637
BseBI CCWGG 1 cut(s) 463
BseDI CCNNGG 1 cut(s) 631
BseGI GGATG 2 cut(s) 98, 626
BseLI CCNNNNNNNGG 1 cut(s) 637
BseMII CTCAG 1 cut(s) 578
BseSI GKGCMC 1 cut(s) 577
BseXI GCAGC 3 cut(s) 307, 632, 635
BseYI CCCAGC 1 cut(s) 568
Bsh1236I CGCG 1 cut(s) 390
BshFI GGCC 1 cut(s) 641
BshNI GGYRCC 1 cut(s) 572
BsiHKAI GWGCWC 1 cut(s) 461
BsiHKCI CYCGRG 1 cut(s) 443
BslFI GGGAC 1 cut(s) 438
BslI CCNNNNNNNGG 1 cut(s) 637
BsmAI GTCTC 2 cut(s) 115, 462
BsmFI GGGAC 1 cut(s) 438
BsnI GGCC 1 cut(s) 641
BsoBI CYCGRG 1 cut(s) 443
Bsp1286I GDGCHC 2 cut(s) 461, 577
Bsp143I GATC 1 cut(s) 514
BspACI CCGC 3 cut(s) 18, 34, 418
BspANI GGCC 1 cut(s) 641
BspCNI CTCAG 1 cut(s) 577
BspFNI CGCG 1 cut(s) 390
BspLI GGNNCC 3 cut(s) 265, 426, 574
BspPI GGATC 1 cut(s) 522
BspT107I GGYRCC 1 cut(s) 572
BspTI CTTAAG 1 cut(s) 23
BssECI CCNNGG 1 cut(s) 631
BssMI GATC 1 cut(s) 514
BssT1I CCWWGG 1 cut(s) 631
Bst2UI CCWGG 1 cut(s) 463
Bst4CI ACNGT 1 cut(s) 355
BstAFI CTTAAG 1 cut(s) 23
BstAPI GCANNNNNTGC 1 cut(s) 206
BstC8I GCNNGC 5 cut(s) 179, 249, 262, 450, 639
BstDEI CTNAG 1 cut(s) 564
BstENI CCTNNNNNAGG 1 cut(s) 635
BstF5I GGATG 2 cut(s) 98, 626
BstFNI CGCG 1 cut(s) 390
BstH2I RGCGCY 1 cut(s) 31
BstHHI GCGC 2 cut(s) 30, 392
BstKTI GATC 1 cut(s) 517
BstMAI GTCTC 2 cut(s) 115, 462
BstMBI GATC 1 cut(s) 514
BstMWI GCNNNNNNNGC 2 cut(s) 206, 257
BstNI CCWGG 1 cut(s) 463
BstNSI RCATGY 1 cut(s) 251
BstSCI CCNGG 1 cut(s) 461
BstSLI GKGCMC 1 cut(s) 577
BstUI CGCG 1 cut(s) 390
BstV1I GCAGC 3 cut(s) 307, 632, 635
BsuRI GGCC 1 cut(s) 641
BtrI CACGTC 1 cut(s) 40
BtsCI GGATG 2 cut(s) 98, 626
Cac8I GCNNGC 5 cut(s) 179, 249, 262, 450, 639
CaiI CAGNNNCTG 1 cut(s) 614
CfoI GCGC 2 cut(s) 30, 392
Cfr13I GGNCC 2 cut(s) 296, 425
CviAII CATG 5 cut(s) 68, 141, 182, 248, 338
DdeI CTNAG 1 cut(s) 564
DpnI GATC 1 cut(s) 516
DpnII GATC 1 cut(s) 514
Eco130I CCWWGG 1 cut(s) 631
Eco147I AGGCCT 1 cut(s) 641
Eco47I GGWCC 2 cut(s) 296, 425
Eco88I CYCGRG 1 cut(s) 443
EcoNI CCTNNNNNAGG 1 cut(s) 635
EcoO109I RGGNCCY 1 cut(s) 425
EcoRI GAATTC 1 cut(s) 239
EcoRII CCWGG 1 cut(s) 461
EcoT14I CCWWGG 1 cut(s) 631
ErhI CCWWGG 1 cut(s) 631
FaeI CATG 5 cut(s) 71, 144, 185, 251, 341
FaqI GGGAC 1 cut(s) 438
FatI CATG 5 cut(s) 67, 140, 181, 247, 337
FblI GTMKAC 1 cut(s) 285
Fnu4HI GCNGC 4 cut(s) 18, 321, 621, 624
FokI GGATG 2 cut(s) 105, 613
Fsp4HI GCNGC 4 cut(s) 18, 321, 621, 624
FspBI CTAG 3 cut(s) 230, 476, 632
GlaI GCGC 2 cut(s) 29, 391
GluI GCNGC 4 cut(s) 18, 321, 621, 624
GsaI CCCAGC 1 cut(s) 572
HaeII RGCGCY 1 cut(s) 31
HaeIII GGCC 1 cut(s) 641
HhaI GCGC 2 cut(s) 30, 392
Hin1II CATG 5 cut(s) 71, 144, 185, 251, 341
Hin6I GCGC 2 cut(s) 28, 390
HinP1I GCGC 2 cut(s) 28, 390
HinfI GANTC 2 cut(s) 224, 560
HphI GGTGA 2 cut(s) 197, 355
Hpy166II GTNNAC 1 cut(s) 286
Hpy188III TCNNGA 2 cut(s) 254, 404
Hpy8I GTNNAC 1 cut(s) 286
HpyAV CCTTC 1 cut(s) 214
HpyCH4III ACNGT 1 cut(s) 355
HpyCH4IV ACGT 2 cut(s) 39, 536
HpyCH4V TGCA 8 cut(s) 81, 181, 209, 260, 323, 545, 620, 666
HpyF10VI GCNNNNNNNGC 2 cut(s) 206, 257
HpyF3I CTNAG 1 cut(s) 564
HpySE526I ACGT 2 cut(s) 39, 536
Hsp92II CATG 5 cut(s) 71, 144, 185, 251, 341
HspAI GCGC 2 cut(s) 28, 390
Kzo9I GATC 1 cut(s) 514
LmnI GCTCC 2 cut(s) 269, 675
Lsp1109I GCAGC 3 cut(s) 307, 632, 635
LweI GCATC 3 cut(s) 83, 247, 635
MaeI CTAG 3 cut(s) 230, 476, 632
MaeII ACGT 2 cut(s) 39, 536
MaeIII GTNAC 3 cut(s) 40, 150, 507
MalI GATC 1 cut(s) 516
MboI GATC 1 cut(s) 514
MfeI CAATTG 1 cut(s) 146
MhlI GDGCHC 2 cut(s) 461, 577
MluCI AATT 6 cut(s) 111, 130, 146, 239, 362, 546
MmeI TCCRAC 1 cut(s) 557
MnlI CCTC 2 cut(s) 210, 534
MseI TTAA 3 cut(s) 24, 504, 549
MslI CAYNNNNRTG 3 cut(s) 91, 102, 176
MspA1I CMGCKG 1 cut(s) 568
MspCI CTTAAG 1 cut(s) 23
MspR9I CCNGG 1 cut(s) 463
MunI CAATTG 1 cut(s) 146
MvaI CCWGG 1 cut(s) 463
MvnI CGCG 1 cut(s) 390
MwoI GCNNNNNNNGC 2 cut(s) 206, 257
NdeII GATC 1 cut(s) 514
NlaIII CATG 5 cut(s) 71, 144, 185, 251, 341
NlaIV GGNNCC 3 cut(s) 265, 426, 574
NmuCI GTSAC 1 cut(s) 40
NspI RCATGY 1 cut(s) 251
OliI CACNNNNGTG 1 cut(s) 176
PaeI GCATGC 1 cut(s) 251
PceI AGGCCT 1 cut(s) 641
PfeI GAWTC 2 cut(s) 224, 560
PkrI GCNGC 4 cut(s) 19, 322, 622, 625
PpuMI RGGWCCY 1 cut(s) 425
Psp5II RGGWCCY 1 cut(s) 425
Psp6I CCWGG 1 cut(s) 461
PspFI CCCAGC 1 cut(s) 568
PspGI CCWGG 1 cut(s) 461
PspN4I GGNNCC 3 cut(s) 265, 426, 574
PspPI GGNCC 2 cut(s) 296, 425
PspPPI RGGWCCY 1 cut(s) 425
PstNI CAGNNNCTG 1 cut(s) 614
PvuII CAGCTG 1 cut(s) 568
RseI CAYNNNNRTG 3 cut(s) 91, 102, 176
SaqAI TTAA 3 cut(s) 24, 504, 549
SatI GCNGC 4 cut(s) 18, 321, 621, 624
Sau3AI GATC 1 cut(s) 514
Sau96I GGNCC 2 cut(s) 296, 425
ScrFI CCNGG 1 cut(s) 463
SduI GDGCHC 2 cut(s) 461, 577
SfaNI GCATC 3 cut(s) 83, 247, 635
SinI GGWCC 2 cut(s) 296, 425
SmiMI CAYNNNNRTG 3 cut(s) 91, 102, 176
SmlI CTYRAG 1 cut(s) 23
SmoI CTYRAG 1 cut(s) 23
SphI GCATGC 1 cut(s) 251
Sse9I AATT 6 cut(s) 111, 130, 146, 239, 362, 546
SseBI AGGCCT 1 cut(s) 641
SsiI CCGC 3 cut(s) 18, 34, 418
SspMI CTAG 3 cut(s) 230, 476, 632
StuI AGGCCT 1 cut(s) 641
StyD4I CCNGG 1 cut(s) 461
StyI CCWWGG 1 cut(s) 631
TaaI ACNGT 1 cut(s) 355
TaiI ACGT 2 cut(s) 42, 539
TaqI TCGA 1 cut(s) 255
TasI AATT 6 cut(s) 111, 130, 146, 239, 362, 546
TauI GCSGC 1 cut(s) 20
TfiI GAWTC 2 cut(s) 224, 560
Tru1I TTAA 3 cut(s) 24, 504, 549
Tru9I TTAA 3 cut(s) 24, 504, 549
TseFI GTSAC 1 cut(s) 40
TseI GCWGC 3 cut(s) 320, 620, 623
Tsp45I GTSAC 1 cut(s) 40
TspDTI ATGAA 2 cut(s) 321, 326
TspGWI ACGGA 1 cut(s) 288
Vha464I CTTAAG 1 cut(s) 23
VpaK11BI GGWCC 2 cut(s) 296, 425
XagI CCTNNNNNAGG 1 cut(s) 635
XapI RAATTY 1 cut(s) 239
XceI RCATGY 1 cut(s) 251
XmaJI CCTAGG 1 cut(s) 631
XmiI GTMKAC 1 cut(s) 285
XspI CTAG 3 cut(s) 230, 476, 632
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.