MD15G1045200.v1.1

CBL-interacting serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0015823
Physical Location & Seq
Forward (+)
3100637 .. 3101044
408 bp
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UTR
Exon/CDS
Intron
MD15G1045200.v1.1.491

Sequence Viewer

Length: 408 bp
ATGAGGATGGTGAAACACCCTAACATCGTCGAGCTCTACGAGGTATTGGCAAGCAAGTCCAAGATCTATTTTGCTATGGATCTAGTCCGCGGTGGTGAGCTCTTCGCCAAAATCGCCAAGGGTCGGTTAAGGGAAGATGTAACCAGAGTCTACTTCCAGCAGCTCATCTCCGCCATAGATTTCTGCCACAACTGCGACGTCTACCATCGGGATCTGAAGCCGGAGAATCCCCTCCTCGATGAGGACGACAATTTGAAGGTCACCGATTTCAGACTCAGTGCTTTCATAGAGCACTTGAAGCAGAACGGGCTTCTTCACACCACATGCGACACGCAGGTGTACGTGGCCCCAGAGGTCATCGGGGGAAAAAAGGTACAATGGGGACAAGGTAGATCTCTGGTCTTGTGA

Protein Analysis

136

Amino Acids

15.52

Weight (kDa)

6.96

Isoelectric Point (pI)

21.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 1 - 124 2.4e-34 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 1 - 124 8.1e-15 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0015823)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G30960
fragaria_vesca FvH4_1g01070
malus_domestica MD15G1045200.v1.1 MD15G1153500.v1.1
prunus_persica Prupe.7G261300_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0085831
rosa_laevigata RLG00000013813 RLG00000015723
rosa_multiflora Rmu_sc0001748.1_g000016
rosa_roxburghii Rroxscaffold_2G00155000
rosa_rugosa Rorug01G0463700
rosa_samantha Rh2BG013500 Rh2CG014000 Rh2DG015100
rosa_wichuraiana Rw2G001130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 325
AatII GACGTC 1 cut(s) 201
Acc36I ACCTGC 1 cut(s) 325
AccI GTMKAC 2 cut(s) 150, 201
AccII CGCG 1 cut(s) 90
AciI CCGC 3 cut(s) 88, 90, 171
AclWI GGATC 2 cut(s) 87, 219
AcuI CTGAAG 1 cut(s) 236
AcyI GRCGYC 1 cut(s) 198
AfaI GTAC 2 cut(s) 341, 375
AfiI CCNNNNNNNGG 2 cut(s) 123, 241
AgsI TTSAA 2 cut(s) 256, 298
AleI CACNNNNGTG 1 cut(s) 335
AluBI AGCT 3 cut(s) 34, 100, 163
AluI AGCT 3 cut(s) 34, 100, 163
Alw21I GWGCWC 3 cut(s) 36, 102, 294
AlwI GGATC 2 cut(s) 87, 219
AoxI GGCC 1 cut(s) 345
ApeKI GCWGC 1 cut(s) 160
AspS9I GGNCC 1 cut(s) 346
AsuHPI GGTGA 3 cut(s) 22, 107, 253
BanII GRGCYC 2 cut(s) 36, 102
Bbv12I GWGCWC 3 cut(s) 36, 102, 294
BbvI GCAGC 1 cut(s) 172
BccI CCATC 1 cut(s) 213
BfaI CTAG 1 cut(s) 83
BfuAI ACCTGC 1 cut(s) 325
BglII AGATCT 2 cut(s) 63, 392
BisI GCNGC 1 cut(s) 161
BlsI GCNGC 1 cut(s) 162
BmgT120I GGNCC 1 cut(s) 346
BmiI GGNNCC 1 cut(s) 348
BsaAI YACGTR 1 cut(s) 343
BsaHI GRCGYC 1 cut(s) 198
BsaJI CCNNGG 2 cut(s) 88, 117
Bsc4I CCNNNNNNNGG 2 cut(s) 123, 241
BseDI CCNNGG 2 cut(s) 88, 117
BseGI GGATG 1 cut(s) 12
BseLI CCNNNNNNNGG 2 cut(s) 123, 241
BseMII CTCAG 1 cut(s) 289
BseRI GAGGAG 1 cut(s) 224
BseXI GCAGC 1 cut(s) 172
Bsh1236I CGCG 1 cut(s) 90
BshFI GGCC 1 cut(s) 347
BsiHKAI GWGCWC 3 cut(s) 36, 102, 294
BsiSI CCGG 1 cut(s) 221
BslFI GGGAC 1 cut(s) 396
BslI CCNNNNNNNGG 2 cut(s) 123, 241
BsmFI GGGAC 1 cut(s) 396
BsnI GGCC 1 cut(s) 347
Bsp1286I GDGCHC 3 cut(s) 36, 102, 294
Bsp143I GATC 4 cut(s) 63, 79, 211, 392
BspACI CCGC 3 cut(s) 88, 90, 171
BspANI GGCC 1 cut(s) 347
BspCNI CTCAG 1 cut(s) 288
BspFNI CGCG 1 cut(s) 90
BspLI GGNNCC 1 cut(s) 348
BspMI ACCTGC 1 cut(s) 325
BspPI GGATC 2 cut(s) 87, 219
BspQI GCTCTTC 1 cut(s) 107
BssECI CCNNGG 2 cut(s) 88, 117
BssMI GATC 4 cut(s) 63, 79, 211, 392
BssNI GRCGYC 1 cut(s) 198
BssT1I CCWWGG 1 cut(s) 117
Bst6I CTCTTC 1 cut(s) 107
BstACI GRCGYC 1 cut(s) 198
BstBAI YACGTR 1 cut(s) 343
BstC8I GCNNGC 1 cut(s) 52
BstDEI CTNAG 1 cut(s) 275
BstDSI CCRYGG 1 cut(s) 88
BstEII GGTNACC 1 cut(s) 259
BstENI CCTNNNNNAGG 1 cut(s) 239
BstF5I GGATG 1 cut(s) 12
BstFNI CGCG 1 cut(s) 90
BstKTI GATC 4 cut(s) 66, 82, 214, 395
BstMBI GATC 4 cut(s) 63, 79, 211, 392
BstMWI GCNNNNNNNGC 4 cut(s) 113, 192, 298, 307
BstNSI RCATGY 1 cut(s) 327
BstPI GGTNACC 1 cut(s) 259
BstUI CGCG 1 cut(s) 90
BstV1I GCAGC 1 cut(s) 172
BstX2I RGATCY 4 cut(s) 63, 79, 211, 392
BstYI RGATCY 4 cut(s) 63, 79, 211, 392
BsuRI GGCC 1 cut(s) 347
BtgI CCRYGG 1 cut(s) 88
BtsCI GGATG 1 cut(s) 12
BtsIMutI CAGTG 1 cut(s) 283
BveI ACCTGC 1 cut(s) 325
Cac8I GCNNGC 1 cut(s) 52
Cfr13I GGNCC 1 cut(s) 346
Cfr42I CCGCGG 1 cut(s) 91
Csp6I GTAC 2 cut(s) 340, 374
CviAII CATG 1 cut(s) 324
CviJI RGCY 6 cut(s) 34, 100, 163, 220, 310, 347
CviKI_1 RGCY 6 cut(s) 34, 100, 163, 220, 310, 347
CviQI GTAC 2 cut(s) 340, 374
DdeI CTNAG 1 cut(s) 275
DpnI GATC 4 cut(s) 65, 81, 213, 394
DpnII GATC 4 cut(s) 63, 79, 211, 392
Eam1104I CTCTTC 1 cut(s) 107
EarI CTCTTC 1 cut(s) 107
EciI GGCGGA 1 cut(s) 160
Ecl136II GAGCTC 2 cut(s) 34, 100
Eco130I CCWWGG 1 cut(s) 117
Eco24I GRGCYC 2 cut(s) 36, 102
Eco53kI GAGCTC 2 cut(s) 34, 100
Eco57I CTGAAG 1 cut(s) 236
Eco91I GGTNACC 1 cut(s) 259
EcoICRI GAGCTC 2 cut(s) 34, 100
EcoNI CCTNNNNNAGG 1 cut(s) 239
EcoO65I GGTNACC 1 cut(s) 259
EcoT14I CCWWGG 1 cut(s) 117
EcoT38I GRGCYC 2 cut(s) 36, 102
ErhI CCWWGG 1 cut(s) 117
FaeI CATG 1 cut(s) 327
FaiI YATR 4 cut(s) 77, 176, 287, 325
FaqI GGGAC 1 cut(s) 396
FatI CATG 1 cut(s) 323
FblI GTMKAC 2 cut(s) 150, 201
Fnu4HI GCNGC 1 cut(s) 161
FokI GGATG 1 cut(s) 19
FriOI GRGCYC 2 cut(s) 36, 102
Fsp4HI GCNGC 1 cut(s) 161
FspBI CTAG 1 cut(s) 83
GluI GCNGC 1 cut(s) 161
HaeIII GGCC 1 cut(s) 347
HapII CCGG 1 cut(s) 221
Hin1I GRCGYC 1 cut(s) 198
Hin1II CATG 1 cut(s) 327
HinfI GANTC 3 cut(s) 147, 226, 273
HpaII CCGG 1 cut(s) 221
HphI GGTGA 3 cut(s) 22, 107, 253
Hpy166II GTNNAC 3 cut(s) 151, 202, 340
Hpy188I TCNGA 2 cut(s) 216, 272
Hpy188III TCNNGA 1 cut(s) 209
Hpy8I GTNNAC 3 cut(s) 151, 202, 340
Hpy99I CGWCG 2 cut(s) 32, 200
HpyAV CCTTC 1 cut(s) 250
HpyCH4IV ACGT 2 cut(s) 198, 342
HpyF10VI GCNNNNNNNGC 4 cut(s) 113, 192, 298, 307
HpyF3I CTNAG 1 cut(s) 275
HpySE526I ACGT 2 cut(s) 198, 342
Hsp92I GRCGYC 1 cut(s) 198
Hsp92II CATG 1 cut(s) 327
KspI CCGCGG 1 cut(s) 91
Kzo9I GATC 4 cut(s) 63, 79, 211, 392
LguI GCTCTTC 1 cut(s) 107
LpnPI CCDG 6 cut(s) 157, 170, 234, 320, 363, 383
Lsp1109I GCAGC 1 cut(s) 172
MaeI CTAG 1 cut(s) 83
MaeII ACGT 2 cut(s) 198, 342
MaeIII GTNAC 2 cut(s) 139, 259
MalI GATC 4 cut(s) 65, 81, 213, 394
MboI GATC 4 cut(s) 63, 79, 211, 392
MboII GAAGA 3 cut(s) 94, 146, 305
MflI RGATCY 4 cut(s) 63, 79, 211, 392
MhlI GDGCHC 3 cut(s) 36, 102, 294
MluCI AATT 1 cut(s) 250
MlyI GAGTC 2 cut(s) 156, 267
MnlI CCTC 5 cut(s) 34, 235, 242, 245, 346
MseI TTAA 1 cut(s) 128
MslI CAYNNNNRTG 1 cut(s) 335
MspA1I CMGCKG 1 cut(s) 90
MspI CCGG 1 cut(s) 221
MvnI CGCG 1 cut(s) 90
MwoI GCNNNNNNNGC 4 cut(s) 113, 192, 298, 307
NdeII GATC 4 cut(s) 63, 79, 211, 392
NlaIII CATG 1 cut(s) 327
NlaIV GGNNCC 1 cut(s) 348
NmuCI GTSAC 1 cut(s) 259
NspI RCATGY 1 cut(s) 327
OliI CACNNNNGTG 1 cut(s) 335
PaqCI CACCTGC 1 cut(s) 325
PciSI GCTCTTC 1 cut(s) 107
PcsI WCGNNNNNNNCGW 2 cut(s) 36, 243
PfeI GAWTC 1 cut(s) 226
PkrI GCNGC 1 cut(s) 162
PleI GAGTC 2 cut(s) 155, 267
PpsI GAGTC 2 cut(s) 155, 267
Ppu21I YACGTR 1 cut(s) 343
Psp124BI GAGCTC 2 cut(s) 36, 102
PspEI GGTNACC 1 cut(s) 259
PspN4I GGNNCC 1 cut(s) 348
PspPI GGNCC 1 cut(s) 346
PsuI RGATCY 4 cut(s) 63, 79, 211, 392
RsaI GTAC 2 cut(s) 341, 375
RsaNI GTAC 2 cut(s) 340, 374
RseI CAYNNNNRTG 1 cut(s) 335
SacI GAGCTC 2 cut(s) 36, 102
SacII CCGCGG 1 cut(s) 91
SapI GCTCTTC 1 cut(s) 107
SaqAI TTAA 1 cut(s) 128
SatI GCNGC 1 cut(s) 161
Sau3AI GATC 4 cut(s) 63, 79, 211, 392
Sau96I GGNCC 1 cut(s) 346
SchI GAGTC 2 cut(s) 156, 267
SduI GDGCHC 3 cut(s) 36, 102, 294
Sfr303I CCGCGG 1 cut(s) 91
SgrBI CCGCGG 1 cut(s) 91
SmiMI CAYNNNNRTG 1 cut(s) 335
Sse9I AATT 1 cut(s) 250
SsiI CCGC 3 cut(s) 88, 90, 171
SspMI CTAG 1 cut(s) 83
SstI GAGCTC 2 cut(s) 36, 102
StyI CCWWGG 1 cut(s) 117
TaiI ACGT 2 cut(s) 201, 345
TaqI TCGA 2 cut(s) 30, 237
TasI AATT 1 cut(s) 250
TfiI GAWTC 1 cut(s) 226
Tru1I TTAA 1 cut(s) 128
Tru9I TTAA 1 cut(s) 128
TscAI CASTG 1 cut(s) 283
TseFI GTSAC 1 cut(s) 259
TseI GCWGC 1 cut(s) 160
Tsp45I GTSAC 1 cut(s) 259
TspDTI ATGAA 1 cut(s) 274
TspRI CASTG 1 cut(s) 283
XagI CCTNNNNNAGG 1 cut(s) 239
XceI RCATGY 1 cut(s) 327
XmiI GTMKAC 2 cut(s) 150, 201
XspI CTAG 1 cut(s) 83
ZraI GACGTC 1 cut(s) 199
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.