Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0015823

Physical Location & Seq

Genomic Coordinates Forward (+)

11383159 .. 11384457

Gene Structure 1299 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1153500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1299 bp

ATGGCCGAGCAGAAAGAAAGCGGCGGCTCGTCGTTGCTGCACGGCAAGTACGAGCTGGGCCGTCTTCTGGGTCACGGCACTTTCGCCAAGGTCTACCACGCCCGACACCTGCCTAGCGGGAAGAACGTGGCGATGAAGGTGGTGGGGAAGGAGAAGGTGATCAAGGTGGGGATGATGGAGCAGATCAAGAGGGAGATCTCCGTGATGAGGATGGTGAAGCACCCCAACATCGTCGAGCTCCACGAGGTCTTGGCGAGCAAGTCCAAGATCTATTTCGCTATGGATCTGGTCCGCGGTGGCGAGCTCTTCGCCAAAATCGCCAAGGGTCGGCTCAGGGAAGACGTCGCCAGAGTCTACTTCCAGCAGCTCATCTCCGCCATCGATTTCTGCCACAGCCGCGGCGTCTACCACCGGGATCTGAAGCCGGAGAACCTCCTCCTCGATGAGGACGGCAATTTGAAGGTCACCGATTTCGGACTCAGTGCTTTCACAGAGCACTTGAAGCAGGACGGGCTTCTCCACACCACATGCGGCACGCCGGCGTACGTGGCCCCCGAGGTCATCGGGAAAAAAGGGTACGATGGTGCCAAGGCAGATCTCTGGTCTTGTGGCGTCATCCTCTACGTGCTTCTCGCCGGGTTTTTGCCGTTTCAAGACGACAATCTCGTGGCCATGTACCGGAAGATTTACAGGGGAGATTTCAAATGCCCGCCGTGGTTTTCGTCGGAGGCACGAAGACTCGTGACGAAGCTTCTCGACCCGAATCCAAGTACCCGAATCACCATTTCCAAGGTCATGGACTCCTCCTGGTTCAAAAAATCGGTTCCTAAAATCGTACGGACGAAACAGGAGCAGGAGTTCGACGAGCCGAGCGAGAAGATCCAGTCGAAGCAGACGGAGACGCTGAACGCGTTTCACATAATCTCGCTGTCGGAGGGGTTCGATTTGTCGCCGCTGTTCGAGGAGAAGAAGCGGGAGGAGAGGGAAGAGCTGCGGTTCGCGACGACGCGGCCGGCGAGCAGCGTGATTTCGAAGCTGGAGGAGGTGGCGGCGGCGGGGAAGTTCAGGATAAAGAAGAGCGACTCGATGGTGAGGTTGCAGGGGCAGGAGAGCGGGCGGAAGGGGAAGCTGGCGATAGCGGCGGAGATATTCGCCATGACGCCGTCGTTTGTAGTGGTGGAGGTGAAGAAGGACAATGGTGATACGTTGGAGTACAACCAGTTCTGCAGCAAGGAGCTGCGGCCAGCGCTCAAGGACATCGTGTGGACATCCCCCGTGGAGAATTCTACGGCGGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko04068 ko04140 ko04150 ko04151 ko04152 ko04211 ko04213 ko04371 ko04530 ko04710 ko04714 ko04910 ko04920 ko04921 ko04922 ko04931 ko04932 ko05410 ko05418 map04068 map04140 map04150 map04151 map04152 map04211 map04213 map04371 map04530 map04710 map04714 map04910 map04920 map04921 map04922 map04931 map04932 map05410 map05418

Pfam Domains

Protein Families

NAF Pkinase

Protein Analysis

433

Amino Acids

48.59

Weight (kDa)

9.12

Isoelectric Point (pI)

36.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	17 - 271	5.9e-74	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	18 - 266	1.1e-33	Protein tyrosine and serine/threonine kinase
NAF	PF03822	300 - 359	8.7e-21	NAF domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015823)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G30960
fragaria_vesca	FvH4_1g01070
malus_domestica	MD15G1045200.v1.1 MD15G1153500.v1.1
prunus_persica	Prupe.7G261300_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0085831
rosa_laevigata	RLG00000013813 RLG00000015723
rosa_multiflora	Rmu_sc0001748.1_g000016
rosa_roxburghii	Rroxscaffold_2G00155000
rosa_rugosa	Rorug01G0463700
rosa_samantha	Rh2BG013500 Rh2CG014000 Rh2DG015100
rosa_wichuraiana	Rw2G001130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	117
AatII	GACGTC	1 cut(s)	345
Acc36I	ACCTGC	1 cut(s)	117
AccB1I	GGYRCC	1 cut(s)	584
AccBSI	CCGCTC	1 cut(s)	1113
AccI	GTMKAC	3 cut(s)	93, 354, 405
AccII	CGCG	5 cut(s)	294, 399, 911, 1001, 1009
AclWI	GGATC	3 cut(s)	291, 423, 874
AcoI	YGGCCR	4 cut(s)	3, 669, 1010, 1241
AcsI	RAATTY	1 cut(s)	1282
AcuI	CTGAAG	1 cut(s)	440
AcyI	GRCGYC	4 cut(s)	342, 402, 612, 1160
AfaI	GTAC	7 cut(s)	50, 545, 578, 677, 772, 837, 1214
AfeI	AGCGCT	1 cut(s)	1248
AfiI	CCNNNNNNNGG	5 cut(s)	67, 207, 327, 445, 678
AflIII	ACRYGT	1 cut(s)	909
AgsI	TTSAA	5 cut(s)	460, 502, 653, 703, 814
AjnI	CCWGG	1 cut(s)	806
AjuI	GAANNNNNNNTTGG	2 cut(s)	257, 289
AloI	GAACNNNNNNTCC	2 cut(s)	842, 874
AluBI	AGCT	9 cut(s)	55, 238, 304, 367, 751, 991, 1036, 1129, 1237
AluI	AGCT	9 cut(s)	55, 238, 304, 367, 751, 991, 1036, 1129, 1237
Alw21I	GWGCWC	3 cut(s)	240, 306, 498
Alw26I	GTCTC	1 cut(s)	893
AlwI	GGATC	3 cut(s)	291, 423, 874
Ama87I	CYCGRG	1 cut(s)	554
Aor51HI	AGCGCT	1 cut(s)	1248
AoxI	GGCC	6 cut(s)	3, 58, 549, 669, 1010, 1241
ApeKI	GCWGC	6 cut(s)	37, 364, 991, 1020, 1227, 1237
ApoI	RAATTY	1 cut(s)	1282
AspLEI	GCGC	1 cut(s)	1249
AspS9I	GGNCC	3 cut(s)	58, 289, 550
AsuC2I	CCSGG	2 cut(s)	413, 637
AsuHPI	GGTGA	7 cut(s)	169, 226, 457, 772, 1102, 1195, 1211
AsuII	TTCGAA	1 cut(s)	1031
AvaI	CYCGRG	1 cut(s)	554
AvaII	GGWCC	1 cut(s)	289
BaeI	ACNNNNGTAYC	2 cut(s)	568, 601
BalI	TGGCCA	1 cut(s)	671
BanI	GGYRCC	1 cut(s)	584
BanII	GRGCYC	2 cut(s)	240, 306
BauI	CACGAG	3 cut(s)	242, 665, 740
BbsI	GAAGAC	3 cut(s)	56, 345, 742
Bbv12I	GWGCWC	3 cut(s)	240, 306, 498
BbvI	GCAGC	6 cut(s)	24, 376, 978, 1032, 1224, 1239
BccI	CCATC	5 cut(s)	169, 205, 386, 575, 1081
BceAI	ACGGC	7 cut(s)	45, 58, 91, 466, 631, 697, 1147
BciT130I	CCWGG	1 cut(s)	808
BclI	TGATCA	1 cut(s)	159
BcnI	CCSGG	2 cut(s)	413, 637
BcoDI	GTCTC	1 cut(s)	893
BfaI	CTAG	1 cut(s)	114
BfmI	CTRYAG	1 cut(s)	1225
BfoI	RGCGCY	1 cut(s)	1250
BfuAI	ACCTGC	1 cut(s)	117
BglII	AGATCT	3 cut(s)	195, 267, 595
Bme1390I	CCNGG	3 cut(s)	413, 637, 808
Bme18I	GGWCC	1 cut(s)	289
BmeT110I	CYCGRG	1 cut(s)	554
BmgT120I	GGNCC	3 cut(s)	58, 289, 550
BmiI	GGNNCC	3 cut(s)	552, 586, 825
BmrFI	CCNGG	3 cut(s)	413, 637, 808
BpiI	GAAGAC	3 cut(s)	56, 345, 742
BpmI	CTGGAG	1 cut(s)	1058
Bpu10I	CCTNAGC	1 cut(s)	332
Bpu14I	TTCGAA	1 cut(s)	1031
BpuEI	CTTGAG	1 cut(s)	1235
BpuMI	CCSGG	2 cut(s)	413, 637
Bsa29I	ATCGAT	1 cut(s)	381
BsaAI	YACGTR	2 cut(s)	547, 625
BsaHI	GRCGYC	4 cut(s)	342, 402, 612, 1160
BsaJI	CCNNGG	9 cut(s)	87, 292, 321, 397, 555, 588, 713, 789, 1275
BsaWI	WCCGGW	1 cut(s)	678
BsaXI	ACNNNNNCTCC	2 cut(s)	842, 872
Bsc4I	CCNNNNNNNGG	5 cut(s)	67, 207, 327, 445, 678
Bse118I	RCCGGY	2 cut(s)	538, 1012
Bse1I	ACTGG	2 cut(s)	883, 1219
BseBI	CCWGG	1 cut(s)	808
BseCI	ATCGAT	1 cut(s)	381
BseDI	CCNNGG	9 cut(s)	87, 292, 321, 397, 555, 588, 713, 789, 1275
BseGI	GGATG	4 cut(s)	177, 216, 615, 1268
BseLI	CCNNNNNNNGG	5 cut(s)	67, 207, 327, 445, 678
BseMII	CTCAG	2 cut(s)	346, 493
BseNI	ACTGG	2 cut(s)	883, 1219
BseRI	GAGGAG	6 cut(s)	425, 428, 793, 977, 992, 1055
BseX3I	CGGCCG	1 cut(s)	1010
BseXI	GCAGC	6 cut(s)	24, 376, 978, 1032, 1224, 1239
BseYI	CCCAGC	1 cut(s)	55
BsgI	GTGCAG	1 cut(s)	23
Bsh1236I	CGCG	5 cut(s)	294, 399, 911, 1001, 1009
Bsh1285I	CGRYCG	1 cut(s)	1013
BshFI	GGCC	6 cut(s)	5, 60, 551, 671, 1012, 1243
BshNI	GGYRCC	1 cut(s)	584
BshVI	ATCGAT	1 cut(s)	381
BsiEI	CGRYCG	1 cut(s)	1013
BsiHKAI	GWGCWC	3 cut(s)	240, 306, 498
BsiHKCI	CYCGRG	1 cut(s)	554
BsiSI	CCGG	6 cut(s)	412, 425, 539, 636, 679, 1013
BsiWI	CGTACG	2 cut(s)	543, 835
BslI	CCNNNNNNNGG	5 cut(s)	67, 207, 327, 445, 678
BsmAI	GTCTC	1 cut(s)	893
BsmBI	CGTCTC	1 cut(s)	893
BsnI	GGCC	6 cut(s)	5, 60, 551, 671, 1012, 1243
BsoBI	CYCGRG	1 cut(s)	554
Bsp119I	TTCGAA	1 cut(s)	1031
Bsp1286I	GDGCHC	3 cut(s)	240, 306, 498
Bsp143I	GATC	8 cut(s)	159, 183, 195, 267, 283, 415, 595, 879
Bsp68I	TCGCGA	1 cut(s)	1001
BspANI	GGCC	6 cut(s)	5, 60, 551, 671, 1012, 1243
BspCNI	CTCAG	2 cut(s)	345, 492
BspDI	ATCGAT	1 cut(s)	381
BspFNI	CGCG	5 cut(s)	294, 399, 911, 1001, 1009
BspLI	GGNNCC	3 cut(s)	552, 586, 825
BspMAI	CTGCAG	1 cut(s)	1229
BspMI	ACCTGC	1 cut(s)	117
BspPI	GGATC	3 cut(s)	291, 423, 874
BspQI	GCTCTTC	3 cut(s)	311, 981, 1070
BspT104I	TTCGAA	1 cut(s)	1031
BspT107I	GGYRCC	1 cut(s)	584
BsrBI	CCGCTC	1 cut(s)	1113
BsrFI	RCCGGY	2 cut(s)	538, 1012
BsrI	ACTGG	2 cut(s)	883, 1219
BssAI	RCCGGY	2 cut(s)	538, 1012
BssECI	CCNNGG	9 cut(s)	87, 292, 321, 397, 555, 588, 713, 789, 1275
BssMI	GATC	8 cut(s)	159, 183, 195, 267, 283, 415, 595, 879
BssNI	GRCGYC	4 cut(s)	342, 402, 612, 1160
BssSI	CACGAG	3 cut(s)	242, 665, 740
BssT1I	CCWWGG	4 cut(s)	87, 321, 588, 789
Bst2BI	CACGAG	3 cut(s)	242, 665, 740
Bst2UI	CCWGG	1 cut(s)	808
Bst6I	CTCTTC	3 cut(s)	311, 981, 1070
BstACI	GRCGYC	4 cut(s)	342, 402, 612, 1160
BstBAI	YACGTR	2 cut(s)	547, 625
BstBI	TTCGAA	1 cut(s)	1031
BstDEI	CTNAG	2 cut(s)	332, 479
BstDSI	CCRYGG	4 cut(s)	292, 397, 713, 1275
BstEII	GGTNACC	1 cut(s)	463
BstENI	CCTNNNNNAGG	1 cut(s)	443
BstF5I	GGATG	4 cut(s)	177, 216, 615, 1268
BstFNI	CGCG	5 cut(s)	294, 399, 911, 1001, 1009
BstH2I	RGCGCY	1 cut(s)	1250
BstHHI	GCGC	1 cut(s)	1249
BstKTI	GATC	8 cut(s)	162, 186, 198, 270, 286, 418, 598, 882
BstMAI	GTCTC	1 cut(s)	893
BstMBI	GATC	8 cut(s)	159, 183, 195, 267, 283, 415, 595, 879
BstMCI	CGRYCG	1 cut(s)	1013
BstMWI	GCNNNNNNNGC	7 cut(s)	317, 396, 502, 511, 548, 1139, 1246
BstNI	CCWGG	1 cut(s)	808
BstNSI	RCATGY	1 cut(s)	531
BstPI	GGTNACC	1 cut(s)	463
BstSCI	CCNGG	3 cut(s)	411, 635, 806
BstSFI	CTRYAG	1 cut(s)	1225
BstUI	CGCG	5 cut(s)	294, 399, 911, 1001, 1009
BstV1I	GCAGC	6 cut(s)	24, 376, 978, 1032, 1224, 1239
BstV2I	GAAGAC	3 cut(s)	56, 345, 742
BstX2I	RGATCY	6 cut(s)	195, 267, 283, 415, 595, 879
BstXI	CCANNNNNNTGG	1 cut(s)	796
BstYI	RGATCY	6 cut(s)	195, 267, 283, 415, 595, 879
BstZI	CGGCCG	1 cut(s)	1010
Bsu15I	ATCGAT	1 cut(s)	381
BsuRI	GGCC	6 cut(s)	5, 60, 551, 671, 1012, 1243
BsuTUI	ATCGAT	1 cut(s)	381
BtgI	CCRYGG	4 cut(s)	292, 397, 713, 1275
BtgZI	GCGATG	1 cut(s)	146
BtsCI	GGATG	4 cut(s)	177, 216, 615, 1268
BtsIMutI	CAGTG	1 cut(s)	487
BtuMI	TCGCGA	1 cut(s)	1001
BveI	ACCTGC	1 cut(s)	117
CfoI	GCGC	1 cut(s)	1249
Cfr10I	RCCGGY	2 cut(s)	538, 1012
Cfr13I	GGNCC	3 cut(s)	58, 289, 550
Cfr42I	CCGCGG	2 cut(s)	295, 400
ClaI	ATCGAT	1 cut(s)	381
CseI	GACGC	5 cut(s)	391, 601, 910, 1015, 1168
Csp6I	GTAC	7 cut(s)	49, 544, 577, 676, 771, 836, 1213
CviAII	CATG	4 cut(s)	528, 673, 796, 1156
CviQI	GTAC	7 cut(s)	49, 544, 577, 676, 771, 836, 1213
DdeI	CTNAG	2 cut(s)	332, 479
DpnI	GATC	8 cut(s)	161, 185, 197, 269, 285, 417, 597, 881
DpnII	GATC	8 cut(s)	159, 183, 195, 267, 283, 415, 595, 879
EaeI	YGGCCR	4 cut(s)	3, 669, 1010, 1241
EagI	CGGCCG	1 cut(s)	1010
Eam1104I	CTCTTC	3 cut(s)	311, 981, 1070
EarI	CTCTTC	3 cut(s)	311, 981, 1070
EciI	GGCGGA	3 cut(s)	364, 1132, 1157
Ecl136II	GAGCTC	2 cut(s)	238, 304
EclXI	CGGCCG	1 cut(s)	1010
Eco130I	CCWWGG	4 cut(s)	87, 321, 588, 789
Eco24I	GRGCYC	2 cut(s)	240, 306
Eco47I	GGWCC	1 cut(s)	289
Eco47III	AGCGCT	1 cut(s)	1248
Eco52I	CGGCCG	1 cut(s)	1010
Eco53kI	GAGCTC	2 cut(s)	238, 304
Eco57I	CTGAAG	1 cut(s)	440
Eco88I	CYCGRG	1 cut(s)	554
Eco91I	GGTNACC	1 cut(s)	463
EcoICRI	GAGCTC	2 cut(s)	238, 304
EcoNI	CCTNNNNNAGG	1 cut(s)	443
EcoO65I	GGTNACC	1 cut(s)	463
EcoRI	GAATTC	1 cut(s)	1282
EcoRII	CCWGG	1 cut(s)	806
EcoT14I	CCWWGG	4 cut(s)	87, 321, 588, 789
EcoT38I	GRGCYC	2 cut(s)	240, 306
ErhI	CCWWGG	4 cut(s)	87, 321, 588, 789
Esp3I	CGTCTC	1 cut(s)	893
FaeI	CATG	4 cut(s)	531, 676, 799, 1159
FaiI	YATR	6 cut(s)	281, 529, 674, 797, 920, 1157
FatI	CATG	4 cut(s)	527, 672, 795, 1155
FauI	CCCGC	5 cut(s)	110, 717, 966, 1048, 1106
FbaI	TGATCA	1 cut(s)	159
FblI	GTMKAC	3 cut(s)	93, 354, 405
FokI	GGATG	4 cut(s)	184, 223, 602, 1255
FriOI	GRGCYC	2 cut(s)	240, 306
FspBI	CTAG	1 cut(s)	114
GlaI	GCGC	1 cut(s)	1248
GsaI	CCCAGC	1 cut(s)	59
GsuI	CTGGAG	1 cut(s)	1058
HaeII	RGCGCY	1 cut(s)	1250
HaeIII	GGCC	6 cut(s)	5, 60, 551, 671, 1012, 1243
HapII	CCGG	6 cut(s)	412, 425, 539, 636, 679, 1013
HgaI	GACGC	5 cut(s)	391, 601, 910, 1015, 1168
HhaI	GCGC	1 cut(s)	1249
Hin1I	GRCGYC	4 cut(s)	342, 402, 612, 1160
Hin1II	CATG	4 cut(s)	531, 676, 799, 1159
Hin6I	GCGC	1 cut(s)	1247
HinP1I	GCGC	1 cut(s)	1247
HindIII	AAGCTT	1 cut(s)	749
HinfI	GANTC	7 cut(s)	351, 477, 738, 763, 777, 800, 1082
HpaII	CCGG	6 cut(s)	412, 425, 539, 636, 679, 1013
HphI	GGTGA	7 cut(s)	169, 226, 457, 772, 1102, 1195, 1211
Hpy166II	GTNNAC	4 cut(s)	94, 355, 406, 1266
Hpy188I	TCNGA	4 cut(s)	420, 476, 727, 934
Hpy188III	TCNNGA	7 cut(s)	187, 565, 653, 742, 755, 1000, 1066
Hpy8I	GTNNAC	4 cut(s)	94, 355, 406, 1266
Hpy99I	CGWCG	8 cut(s)	34, 236, 347, 727, 866, 1006, 1009, 1168
HpyAV	CCTTC	6 cut(s)	130, 142, 148, 454, 1114, 1183
HpyCH4IV	ACGT	5 cut(s)	126, 342, 546, 624, 1205
HpyCH4V	TGCA	3 cut(s)	40, 1099, 1227
HpyF10VI	GCNNNNNNNGC	7 cut(s)	317, 396, 502, 511, 548, 1139, 1246
HpyF3I	CTNAG	2 cut(s)	332, 479
HpySE526I	ACGT	5 cut(s)	126, 342, 546, 624, 1205
Hsp92I	GRCGYC	4 cut(s)	342, 402, 612, 1160
Hsp92II	CATG	4 cut(s)	531, 676, 799, 1159
HspAI	GCGC	1 cut(s)	1247
KroI	GCCGGC	2 cut(s)	538, 1012
KroNI	GCCGGC	2 cut(s)	540, 1014
Ksp22I	TGATCA	1 cut(s)	159
KspI	CCGCGG	2 cut(s)	295, 400
Kzo9I	GATC	8 cut(s)	159, 183, 195, 267, 283, 415, 595, 879
LguI	GCTCTTC	3 cut(s)	311, 981, 1070
LmnI	GCTCC	4 cut(s)	178, 243, 850, 1234
Lsp1109I	GCAGC	6 cut(s)	24, 376, 978, 1032, 1224, 1239
MaeI	CTAG	1 cut(s)	114
MaeII	ACGT	5 cut(s)	126, 342, 546, 624, 1205
MaeIII	GTNAC	3 cut(s)	71, 463, 742
MalI	GATC	8 cut(s)	161, 185, 197, 269, 285, 417, 597, 881
MbiI	CCGCTC	1 cut(s)	1113
MboI	GATC	8 cut(s)	159, 183, 195, 267, 283, 415, 595, 879
MflI	RGATCY	6 cut(s)	195, 267, 283, 415, 595, 879
MhlI	GDGCHC	3 cut(s)	240, 306, 498
MlsI	TGGCCA	1 cut(s)	671
MluCI	AATT	2 cut(s)	454, 1282
MluI	ACGCGT	1 cut(s)	909
MluNI	TGGCCA	1 cut(s)	671
MlyI	GAGTC	5 cut(s)	360, 471, 732, 794, 1076
MmeI	TCCRAC	3 cut(s)	705, 912, 1188
Mox20I	TGGCCA	1 cut(s)	671
MreI	CGCCGGCG	1 cut(s)	538
MroNI	GCCGGC	2 cut(s)	538, 1012
MscI	TGGCCA	1 cut(s)	671
Msp20I	TGGCCA	1 cut(s)	671
MspA1I	CMGCKG	3 cut(s)	294, 399, 955
MspI	CCGG	6 cut(s)	412, 425, 539, 636, 679, 1013
MspR9I	CCNGG	3 cut(s)	413, 637, 808
MvaI	CCWGG	1 cut(s)	808
MvnI	CGCG	5 cut(s)	294, 399, 911, 1001, 1009
MwoI	GCNNNNNNNGC	7 cut(s)	317, 396, 502, 511, 548, 1139, 1246
NaeI	GCCGGC	2 cut(s)	540, 1014
NciI	CCSGG	2 cut(s)	413, 637
NdeII	GATC	8 cut(s)	159, 183, 195, 267, 283, 415, 595, 879
NgoMIV	GCCGGC	2 cut(s)	538, 1012
NlaIII	CATG	4 cut(s)	531, 676, 799, 1159
NlaIV	GGNNCC	3 cut(s)	552, 586, 825
NmeAIII	GCCGAG	2 cut(s)	31, 894
NmuCI	GTSAC	3 cut(s)	71, 463, 742
NruI	TCGCGA	1 cut(s)	1001
NspI	RCATGY	1 cut(s)	531
NspV	TTCGAA	1 cut(s)	1031
PaqCI	CACCTGC	1 cut(s)	117
PciSI	GCTCTTC	3 cut(s)	311, 981, 1070
PcsI	WCGNNNNNNNCGW	7 cut(s)	48, 240, 552, 663, 870, 908, 1013
PdiI	GCCGGC	2 cut(s)	540, 1014
PfeI	GAWTC	2 cut(s)	763, 777
Pfl23II	CGTACG	2 cut(s)	543, 835
PflFI	GACNNNGTC	1 cut(s)	1162
PleI	GAGTC	5 cut(s)	359, 471, 732, 794, 1076
PpsI	GAGTC	5 cut(s)	359, 471, 732, 794, 1076
Ppu21I	YACGTR	2 cut(s)	547, 625
Psp124BI	GAGCTC	2 cut(s)	240, 306
Psp6I	CCWGG	1 cut(s)	806
PspEI	GGTNACC	1 cut(s)	463
PspFI	CCCAGC	1 cut(s)	55
PspGI	CCWGG	1 cut(s)	806
PspLI	CGTACG	2 cut(s)	543, 835
PspN4I	GGNNCC	3 cut(s)	552, 586, 825
PspPI	GGNCC	3 cut(s)	58, 289, 550
PsrI	GAACNNNNNNTAC	2 cut(s)	1205, 1237
PstI	CTGCAG	1 cut(s)	1229
PsuI	RGATCY	6 cut(s)	195, 267, 283, 415, 595, 879
PsyI	GACNNNGTC	1 cut(s)	1162
RruI	TCGCGA	1 cut(s)	1001
RsaI	GTAC	7 cut(s)	50, 545, 578, 677, 772, 837, 1214
RsaNI	GTAC	7 cut(s)	49, 544, 577, 676, 771, 836, 1213
SacI	GAGCTC	2 cut(s)	240, 306
SacII	CCGCGG	2 cut(s)	295, 400
SapI	GCTCTTC	3 cut(s)	311, 981, 1070
Sau3AI	GATC	8 cut(s)	159, 183, 195, 267, 283, 415, 595, 879
Sau96I	GGNCC	3 cut(s)	58, 289, 550
SchI	GAGTC	5 cut(s)	360, 471, 732, 794, 1076
ScrFI	CCNGG	3 cut(s)	413, 637, 808
SduI	GDGCHC	3 cut(s)	240, 306, 498
SfcI	CTRYAG	1 cut(s)	1225
Sfr303I	CCGCGG	2 cut(s)	295, 400
SfuI	TTCGAA	1 cut(s)	1031
SgrAI	CRCCGGYG	1 cut(s)	538
SgrBI	CCGCGG	2 cut(s)	295, 400
SinI	GGWCC	1 cut(s)	289
SmlI	CTYRAG	1 cut(s)	1250
SmoI	CTYRAG	1 cut(s)	1250
Sse9I	AATT	2 cut(s)	454, 1282
SspMI	CTAG	1 cut(s)	114
SstI	GAGCTC	2 cut(s)	240, 306
StyD4I	CCNGG	3 cut(s)	411, 635, 806
StyI	CCWWGG	4 cut(s)	87, 321, 588, 789
TaiI	ACGT	5 cut(s)	129, 345, 549, 627, 1208
TasI	AATT	2 cut(s)	454, 1282
TatI	WGTACW	1 cut(s)	1212
TfiI	GAWTC	2 cut(s)	763, 777
TscAI	CASTG	1 cut(s)	487
TseFI	GTSAC	3 cut(s)	71, 463, 742
TseI	GCWGC	6 cut(s)	37, 364, 991, 1020, 1227, 1237
Tsp45I	GTSAC	3 cut(s)	71, 463, 742
TspDTI	ATGAA	1 cut(s)	149
TspGWI	ACGGA	3 cut(s)	190, 853, 911
TspRI	CASTG	1 cut(s)	487
Tth111I	GACNNNGTC	1 cut(s)	1162
VpaK11BI	GGWCC	1 cut(s)	289
XagI	CCTNNNNNAGG	1 cut(s)	443
XapI	RAATTY	1 cut(s)	1282
XceI	RCATGY	1 cut(s)	531
XmiI	GTMKAC	3 cut(s)	93, 354, 405
XspI	CTAG	1 cut(s)	114
ZraI	GACGTC	1 cut(s)	343

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.