Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0008606

Physical Location & Seq

Genomic Coordinates Reverse (-)

3395833 .. 3402372

Gene Structure 6540 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1049900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3018 bp

ATGAAGATTTGGTGCTGCCTATGCTTCACCCAAGGAGACGAACCAGAAGAGGACGGTAGCGAGGCGAAGGCGATCATGAACGAGGGGGATTTCGGAAATGACGGCAATTCGGAGGTCGCCGTTGGGAATGGCGACGAAATTGACGGCAATGCTCAGAGTTTGGGGCTGGTGGGGAATGAGCGCGGCCGTGACGACCATCTGAGATTGTTTGAAGGGATGGTTCAGGCAATGCGCGGCGGCGGGGCTCAGTGGGACGAGTCTCTGTGTGTTGGCGCTCTTGCGTCGTTGAGGGCCTCAATTAGGAACCCTCGGCTTTCCGAAGGCGAGAGTAGCTCGGCTGCAGACGATGGTGGTGATCACGATTCACACCATAAGCGAGCCAAAGTTCAATCCTTTAATCATGATTTCCATTATGCAATGGCAATGTCTTCAGGTGCGGGAAATTCCAGTTCATCTGCAGAGAGAGACTACAGAATAAATCAGAGCTCTTTTGTTCCATTTAAGAGTGAGACATTCTTTCAAGATACTACTACTAATAATGGCGGTGAGGAGGGCCCCTTTGATTCTGGAAGTGGTAAGGATGATGAAGGGGATAATAGTGGCACCTCAAAAACAGAAGATTTAGAAGTTCGGATGGATCTTACAGATGACCTGTTACATATGGTTTTCTCTTTCTTGGACCACATTAATCTTTGCCGAGCTGCAATAGTCTGCAGGCAGTGGCGATCTGCTAGTGCTCATGAAGATTTCTGGAGGTGTCTGAATTTTGAGAATCGAAGTATATCTGTGGATCAATTTGAGGACATATGTTGGCGGTATCCCAATGCCACAGAATTGAATATTTCTGGTACCCCTGCGATTCACTTGCTTGTCATGAAAGCTATCTCTTCATTGAGGAATCTTGAGGTTTTAATCCTAGGTAAAGGGCAACTAGGGGACATCTTTTTCCATTCCTTGGCAGACTGTCAAATGTTGAAAAGTTTGATCATCAATGATGCTACTCTCGGTAATGGTATCCAGGAGATACCTATAAACCATGATAGACTCTGTCATCTTCAATTAACAAAATGTCGTGTTATGCGCATATCCATCAGGTGTCCGCAACTTGAAACTCTGTCATTGAAGCGCAGTAACATGGCGCAGGCTGTTCTTAATAGCCCTCTTTTGCATGATGTTGATATAGGCTCTTGCCACAAGCTGTCAGATGCTGCAATTCGTTCAGCAGCAACTTCATGTCCTCAATTAGAGTCTTTAGATATGTCAAATTGTTCATGTGTTAGTGATGAAACTCTACGTGAGATTGCTCTTACTTGTGCTAATCTTCATGTTCTCAATGCGTCATACTGTCCAAATATATCCCTTGAGTCTGTAAGACTGCCAATGTTGACGGTTCTTAAGCTTCACAGTTGTGAGGGCATCAGTTCGGCTTCAATGGTTGCGATATCTCATAGTTACATGCTTGAGGTTTTGGAGCTTGACAATTGCAGTCTACTTACTGCTGTCAACTTGGATCTTCCACGCTTGCAGAATATTAGGCTAGTACATTGTCGCAAGTTTGCTGATTTGAATCTACGATGCATCATGCTGTCATCAATAATGGTCTCTAACTGTCCTGTGCTCCATCGTATCAACATCACTTCAAATTCACTTCAGAAACTAGCTCTGCAAAAGCAAGAGAGCTTAACCACGCTGGCACTGCAATGCCAAAGTTTGCAAGAAGTGGACCTTACAGATTGTGAATCTTTAACAAATTCTATATGTGATGTTTTCAGTGATGGTGGTGGGTGTCCTTTGCTCAAAACATTGGTTCTCGAAAACTGTGAGAGCTTAACCGCAGTTCGATTCTGCAGCACTTCTATAGTCAGCCTCTCACTTGTTGGTTGCCGGGCAATTACTTCTCTCGAACTGACTTGCCCCTATCTTGAACAAGTTTCTTTAGATGGTTGTGATCATCTTGAAAGAGCAGAATTTTGTCCAGTTGGTCTTAGGTCACTAAATTTGGGAATATGTCCCAAATTGAATGTGCTCAGTATCGAAGCGCCGAATATGGTGCTGCTTGAGCTGAAGGGATGTGGTGTGTTAGCTGAAGTATCCATCAATTGTCCATTATTAACGTCTCTGGACGCTTCCTTTTGCAGTCAGCTTAGGGATGATTGCTTGTCTGCAACTGCTGCTTCATGCCCGCTGATTGAGTCTTTAATTCTAATGTCATGCCCCTCTGTTGGTTCTGATGGACTCTACTCTTTGCGCTGTCTTCCAAATTTAATTGCGCTTGATTTGTCCTACACTTTCTTGATGAACTTGAAGCCAGTTTTCGAGTCTTGTATGAAACTTAAGGTGTTAAAGTTACAAGCCTGCAAGTACTTATCTGACTCATCCTTGGAGCCTCTTTACAAGGAAGGGGCTCTTCCAGCTCTCCAAGAACTGGATTTGTCTTATGGAACCCTCTGTCAGTCTGCTATCGAGGAGCTTCTTTCTTTTTGTACGCACTTAACTCATGTGAGCTTGAATGGCTGTGTGAACATGCATGACCTGGATTGGGGTAGCAGTGCCGGGCAACCTGCTGCCCTATCTGGCATGTTTTTACCTGAGAATGTCCAGGTTCCAATTGAGCAGCCAAACCGGTTACTGCAGAACCTCAACTGTGTAGGCTGTCCAAATATTAGGAAAGTTGTCATTCCACAAGCAGCACGTTGTTTCCACATGTCGTCATTAAACCTTTCTCTTTCTGCAAATTTAAAGGACGTTGATGTCGCTTGTTTCAACCTCTGCTTCCTTAATTTGAGCAATTGTACTTCTTTGGAAGTTTTGAAGCTTGACTGTCCGAAATTGACTAGTCTCTTTCTTCAGTCCTGCAACATTGATGAAGCAGCAGTGGAAGCTGCCATATCTAAATGTAGCATGCTGGAGACTCTTGATGTTCGTTTTTGTCCCAAGATAAGCCCGATGAGCATGGGGAAATTACGTGCTGCATGCCCTAATTTGAAGCGCATCTTCAGCAGCCAGCAACAGTCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0008150 GO:0009653 GO:0010252 GO:0032502 GO:0042592 GO:0048646 GO:0048856 GO:0048878 GO:0065007 GO:0065008 GO:1905393

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

F-box F-box-like

Protein Analysis

1006

Amino Acids

109.72

Weight (kDa)

5.01

Isoelectric Point (pI)

49.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
F-box	PF00646	213 - 252	1.2e-09	F-box domain
F-box-like	PF12937	216 - 256	8.4e-10	F-box-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008606)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G33210
fragaria_vesca	FvH4_2g30970 FvH4_2g30970 FvH4_2g30970 FvH4_2g30970 FvH4_2g30970 FvH4_2g30970 FvH4_2g30970
malus_domestica	MD08G1071300.v1.1 MD15G1049900.v1.1
prunus_persica	Prupe.1G404200_v2.0.a1
pyrus_communis	pycom08g05910 pycom08g05920 pycom15g04850
rosa_chinensis	RchiOBHm_Chr6g0311801
rosa_laevigata	RLG00000010373
rosa_multiflora	Rmu_sc0002888.1_g000043
rosa_roxburghii	Rroxscaffold_7G00157300
rosa_samantha	Rh6AG507100 Rh6BG518100 Rh6CG523600 Rh6DG509400
rosa_wichuraiana	Rw6G044120

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2750
Acc16I	TGCGCA	1 cut(s)	1082
Acc36I	ACCTGC	1 cut(s)	2569
Acc65I	GGTACC	1 cut(s)	848
AccB1I	GGYRCC	2 cut(s)	602, 848
AccB7I	CCANNNNNTGG	2 cut(s)	955, 2425
AccI	GTMKAC	1 cut(s)	1489
AccII	CGCG	2 cut(s)	183, 234
AclWI	GGATC	3 cut(s)	645, 798, 1518
AcoI	YGGCCR	1 cut(s)	184
AcsI	RAATTY	8 cut(s)	442, 763, 1642, 1750, 1967, 1996, 2260, 2734
AcuI	CTGAAG	6 cut(s)	414, 1634, 2084, 2106, 2831, 2980
AfaI	GTAC	5 cut(s)	850, 1542, 2363, 2485, 2794
AfiI	CCNNNNNNNGG	5 cut(s)	300, 955, 2222, 2425, 2539
AflII	CTTAAG	2 cut(s)	1394, 2333
AflIII	ACRYGT	1 cut(s)	2703
AgeI	ACCGGT	1 cut(s)	2622
AhlI	ACTAGT	1 cut(s)	2834
AjnI	CCWGG	3 cut(s)	1017, 2532, 2598
AleI	CACNNNNGTG	1 cut(s)	1407
Alw21I	GWGCWC	4 cut(s)	488, 739, 1620, 2028
Alw26I	GTCTC	8 cut(s)	30, 264, 459, 503, 1606, 2121, 2843, 2903
AlwI	GGATC	3 cut(s)	645, 798, 1518
AlwNI	CAGNNNCTG	1 cut(s)	1208
AoxI	GGCC	3 cut(s)	184, 291, 553
ApaI	GGGCCC	1 cut(s)	557
ApoI	RAATTY	8 cut(s)	442, 763, 1642, 1750, 1967, 1996, 2260, 2734
AseI	ATTAAT	1 cut(s)	687
AsiGI	ACCGGT	1 cut(s)	2622
Asp718I	GGTACC	1 cut(s)	848
AspA2I	CCTAGG	1 cut(s)	916
AspS9I	GGNCC	5 cut(s)	291, 553, 554, 679, 1723
AsuC2I	CCSGG	2 cut(s)	1886, 2554
AsuHPI	GGTGA	3 cut(s)	19, 365, 557
AvaII	GGWCC	2 cut(s)	679, 1723
AvrII	CCTAGG	1 cut(s)	916
BaeGI	GKGCMC	1 cut(s)	557
BanI	GGYRCC	2 cut(s)	602, 848
BanII	GRGCYC	4 cut(s)	247, 488, 557, 2407
BbsI	GAAGAC	2 cut(s)	420, 2246
Bbv12I	GWGCWC	4 cut(s)	488, 739, 1620, 2028
BceAI	ACGGC	4 cut(s)	104, 118, 160, 171
BcgI	CGANNNNNNTGC	4 cut(s)	847, 881, 2032, 2066
BciT130I	CCWGG	3 cut(s)	1019, 2534, 2600
BciVI	GTATCC	3 cut(s)	828, 1025, 2101
BclI	TGATCA	3 cut(s)	355, 984, 1948
BcnI	CCSGG	2 cut(s)	1886, 2554
BcoDI	GTCTC	8 cut(s)	30, 264, 459, 503, 1606, 2121, 2843, 2903
BcuI	ACTAGT	1 cut(s)	2834
BfaI	CTAG	6 cut(s)	732, 917, 932, 1538, 1658, 2835
BfmI	CTRYAG	7 cut(s)	339, 456, 469, 712, 1846, 1857, 2630
BfoI	RGCGCY	2 cut(s)	276, 2042
BfrI	CTTAAG	2 cut(s)	1394, 2333
BfuAI	ACCTGC	1 cut(s)	2569
BfuI	GTATCC	3 cut(s)	828, 1025, 2101
BlnI	CCTAGG	1 cut(s)	916
BmcAI	AGTACT	1 cut(s)	2363
Bme1390I	CCNGG	5 cut(s)	1019, 1886, 2534, 2554, 2600
Bme18I	GGWCC	2 cut(s)	679, 1723
BmgT120I	GGNCC	5 cut(s)	291, 553, 554, 679, 1723
BmiI	GGNNCC	8 cut(s)	305, 555, 556, 604, 850, 2385, 2443, 2604
BmrFI	CCNGG	5 cut(s)	1019, 1886, 2534, 2554, 2600
BmsI	GCATC	6 cut(s)	985, 1195, 1425, 1565, 1587, 3000
BpiI	GAAGAC	2 cut(s)	420, 2246
BplI	GAGNNNNNCTC	2 cut(s)	317, 349
BpmI	CTGGAG	2 cut(s)	772, 2927
Bpu10I	CCTNAGC	1 cut(s)	2144
BpuEI	CTTGAG	4 cut(s)	923, 1382, 1481, 2078
BpuMI	CCSGG	2 cut(s)	1886, 2554
BsaAI	YACGTR	2 cut(s)	1295, 2966
BsaBI	GATNNNNATC	1 cut(s)	1566
BsaI	GGTCTC	1 cut(s)	1606
BsaJI	CCNNGG	5 cut(s)	31, 308, 916, 954, 2379
BsaWI	WCCGGW	1 cut(s)	2622
Bsc4I	CCNNNNNNNGG	5 cut(s)	300, 955, 2222, 2425, 2539
Bse118I	RCCGGY	1 cut(s)	2622
Bse1I	ACTGG	4 cut(s)	447, 1976, 2309, 2430
Bse3DI	GCAATG	5 cut(s)	154, 234, 423, 429, 1706
Bse8I	GATNNNNATC	1 cut(s)	1566
BseBI	CCWGG	3 cut(s)	1019, 2534, 2600
BseDI	CCNNGG	5 cut(s)	31, 308, 916, 954, 2379
BseGI	GGATG	6 cut(s)	222, 586, 639, 2075, 2155, 2375
BseJI	GATNNNNATC	1 cut(s)	1566
BseLI	CCNNNNNNNGG	5 cut(s)	300, 955, 2222, 2425, 2539
BseMI	GCAATG	5 cut(s)	154, 234, 423, 429, 1706
BseMII	CTCAG	5 cut(s)	167, 191, 260, 2041, 2580
BseNI	ACTGG	4 cut(s)	447, 1976, 2309, 2430
BseRI	GAGGAG	2 cut(s)	563, 2480
BseSI	GKGCMC	1 cut(s)	557
BseX3I	CGGCCG	1 cut(s)	184
Bsh1236I	CGCG	2 cut(s)	183, 234
Bsh1285I	CGRYCG	1 cut(s)	187
BshFI	GGCC	3 cut(s)	186, 293, 555
BshNI	GGYRCC	2 cut(s)	602, 848
BshTI	ACCGGT	1 cut(s)	2622
BsiEI	CGRYCG	1 cut(s)	187
BsiHKAI	GWGCWC	4 cut(s)	488, 739, 1620, 2028
BsiSI	CCGG	3 cut(s)	1885, 2553, 2623
BslFI	GGGAC	4 cut(s)	266, 950, 1995, 2916
BslI	CCNNNNNNNGG	5 cut(s)	300, 955, 2222, 2425, 2539
BsmAI	GTCTC	8 cut(s)	30, 264, 459, 503, 1606, 2121, 2843, 2903
BsmBI	CGTCTC	2 cut(s)	30, 2121
BsmFI	GGGAC	4 cut(s)	266, 950, 1995, 2916
BsnI	GGCC	3 cut(s)	186, 293, 555
Bso31I	GGTCTC	1 cut(s)	1606
Bsp120I	GGGCCC	1 cut(s)	553
Bsp1286I	GDGCHC	7 cut(s)	247, 488, 557, 739, 1620, 2028, 2407
Bsp143I	GATC	8 cut(s)	72, 355, 637, 725, 790, 984, 1510, 1948
BspANI	GGCC	3 cut(s)	186, 293, 555
BspCNI	CTCAG	5 cut(s)	166, 192, 259, 2040, 2581
BspFNI	CGCG	2 cut(s)	183, 234
BspHI	TCATGA	4 cut(s)	75, 400, 739, 873
BspLI	GGNNCC	8 cut(s)	305, 555, 556, 604, 850, 2385, 2443, 2604
BspMAI	CTGCAG	5 cut(s)	343, 460, 716, 1850, 2634
BspMI	ACCTGC	1 cut(s)	2569
BspPI	GGATC	3 cut(s)	645, 798, 1518
BspQI	GCTCTTC	1 cut(s)	2412
BspT107I	GGYRCC	2 cut(s)	602, 848
BspTI	CTTAAG	2 cut(s)	1394, 2333
BspTNI	GGTCTC	1 cut(s)	1606
BsrDI	GCAATG	5 cut(s)	154, 234, 423, 429, 1706
BsrFI	RCCGGY	1 cut(s)	2622
BsrI	ACTGG	4 cut(s)	447, 1976, 2309, 2430
BssAI	RCCGGY	1 cut(s)	2622
BssECI	CCNNGG	5 cut(s)	31, 308, 916, 954, 2379
BssMI	GATC	8 cut(s)	72, 355, 637, 725, 790, 984, 1510, 1948
BssT1I	CCWWGG	4 cut(s)	31, 916, 954, 2379
Bst2UI	CCWGG	3 cut(s)	1019, 2534, 2600
Bst6I	CTCTTC	3 cut(s)	42, 892, 2412
BstAFI	CTTAAG	2 cut(s)	1394, 2333
BstAPI	GCANNNNNTGC	1 cut(s)	2171
BstBAI	YACGTR	2 cut(s)	1295, 2966
BstDEI	CTNAG	7 cut(s)	153, 200, 246, 1985, 2027, 2144, 2589
BstENI	CCTNNNNNAGG	1 cut(s)	298
BstF5I	GGATG	6 cut(s)	222, 586, 639, 2075, 2155, 2375
BstFNI	CGCG	2 cut(s)	183, 234
BstH2I	RGCGCY	2 cut(s)	276, 2042
BstKTI	GATC	8 cut(s)	75, 358, 640, 728, 793, 987, 1513, 1951
BstMAI	GTCTC	8 cut(s)	30, 264, 459, 503, 1606, 2121, 2843, 2903
BstMBI	GATC	8 cut(s)	72, 355, 637, 725, 790, 984, 1510, 1948
BstMCI	CGRYCG	1 cut(s)	187
BstNI	CCWGG	3 cut(s)	1019, 2534, 2600
BstNSI	RCATGY	6 cut(s)	1459, 2527, 2581, 2707, 2905, 2976
BstSCI	CCNGG	5 cut(s)	1017, 1884, 2532, 2552, 2598
BstSFI	CTRYAG	7 cut(s)	339, 456, 469, 712, 1846, 1857, 2630
BstSLI	GKGCMC	1 cut(s)	557
BstUI	CGCG	2 cut(s)	183, 234
BstV2I	GAAGAC	2 cut(s)	420, 2246
BstX2I	RGATCY	2 cut(s)	637, 1510
BstYI	RGATCY	2 cut(s)	637, 1510
BstZI	CGGCCG	1 cut(s)	184
BsuI	GTATCC	3 cut(s)	828, 1025, 2101
BsuRI	GGCC	3 cut(s)	186, 293, 555
BtsCI	GGATG	6 cut(s)	222, 586, 639, 2075, 2155, 2375
BtsI	GCAGTG	4 cut(s)	725, 1694, 2554, 2880
BtsIMutI	CAGTG	6 cut(s)	254, 725, 1694, 1777, 2554, 2880
BveI	ACCTGC	1 cut(s)	2569
CaiI	CAGNNNCTG	1 cut(s)	1208
CciI	TCATGA	4 cut(s)	75, 400, 739, 873
Cfr10I	RCCGGY	1 cut(s)	2622
Cfr13I	GGNCC	5 cut(s)	291, 553, 554, 679, 1723
CseI	GACGC	3 cut(s)	270, 1326, 2132
Csp6I	GTAC	5 cut(s)	849, 1541, 2362, 2484, 2793
CspAI	ACCGGT	1 cut(s)	2622
CviQI	GTAC	5 cut(s)	849, 1541, 2362, 2484, 2793
DdeI	CTNAG	7 cut(s)	153, 200, 246, 1985, 2027, 2144, 2589
DpnI	GATC	8 cut(s)	74, 357, 639, 727, 792, 986, 1512, 1950
DpnII	GATC	8 cut(s)	72, 355, 637, 725, 790, 984, 1510, 1948
DraI	TTTAAA	1 cut(s)	2739
DrdI	GACNNNNNNGTC	1 cut(s)	2750
DseDI	GACNNNNNNGTC	1 cut(s)	2750
EaeI	YGGCCR	1 cut(s)	184
EagI	CGGCCG	1 cut(s)	184
Eam1104I	CTCTTC	3 cut(s)	42, 892, 2412
EarI	CTCTTC	3 cut(s)	42, 892, 2412
Ecl136II	GAGCTC	1 cut(s)	486
EclXI	CGGCCG	1 cut(s)	184
Eco130I	CCWWGG	4 cut(s)	31, 916, 954, 2379
Eco24I	GRGCYC	4 cut(s)	247, 488, 557, 2407
Eco31I	GGTCTC	1 cut(s)	1606
Eco32I	GATATC	1 cut(s)	1443
Eco47I	GGWCC	2 cut(s)	679, 1723
Eco52I	CGGCCG	1 cut(s)	184
Eco53kI	GAGCTC	1 cut(s)	486
Eco57I	CTGAAG	6 cut(s)	414, 1634, 2084, 2106, 2831, 2980
EcoICRI	GAGCTC	1 cut(s)	486
EcoNI	CCTNNNNNAGG	1 cut(s)	298
EcoO109I	RGGNCCY	3 cut(s)	291, 553, 554
EcoRII	CCWGG	3 cut(s)	1017, 2532, 2598
EcoRV	GATATC	1 cut(s)	1443
EcoT14I	CCWWGG	4 cut(s)	31, 916, 954, 2379
EcoT22I	ATGCAT	2 cut(s)	1580, 2529
EcoT38I	GRGCYC	4 cut(s)	247, 488, 557, 2407
ErhI	CCWWGG	4 cut(s)	31, 916, 954, 2379
Esp3I	CGTCTC	2 cut(s)	30, 2121
FalI	AAGNNNNNCTT	6 cut(s)	1710, 1742, 2391, 2423, 2978, 3010
FaqI	GGGAC	4 cut(s)	266, 950, 1995, 2916
FauI	CCCGC	3 cut(s)	233, 430, 2190
FauNDI	CATATG	2 cut(s)	660, 806
FbaI	TGATCA	3 cut(s)	355, 984, 1948
FblI	GTMKAC	1 cut(s)	1489
FokI	GGATG	6 cut(s)	229, 593, 646, 2082, 2162, 2362
FriOI	GRGCYC	4 cut(s)	247, 488, 557, 2407
FspAI	RTGCGCAY	1 cut(s)	1082
FspBI	CTAG	6 cut(s)	732, 917, 932, 1538, 1658, 2835
FspI	TGCGCA	1 cut(s)	1082
GsuI	CTGGAG	2 cut(s)	772, 2927
HaeII	RGCGCY	2 cut(s)	276, 2042
HaeIII	GGCC	3 cut(s)	186, 293, 555
HapII	CCGG	3 cut(s)	1885, 2553, 2623
HgaI	GACGC	3 cut(s)	270, 1326, 2132
HincII	GTYRAC	2 cut(s)	1386, 1504
HindII	GTYRAC	2 cut(s)	1386, 1504
HindIII	AAGCTT	2 cut(s)	1397, 2813
HpaII	CCGG	3 cut(s)	1885, 2553, 2623
HphI	GGTGA	3 cut(s)	19, 365, 557
Hpy166II	GTNNAC	5 cut(s)	1386, 1490, 1504, 1723, 2521
Hpy8I	GTNNAC	5 cut(s)	1386, 1490, 1504, 1723, 2521
Hpy99I	CGWCG	2 cut(s)	137, 286
HpyAV	CCTTC	6 cut(s)	61, 206, 314, 581, 2059, 2393
HpyCH4IV	ACGT	5 cut(s)	1294, 2114, 2692, 2745, 2965
HpyF3I	CTNAG	7 cut(s)	153, 200, 246, 1985, 2027, 2144, 2589
HpySE526I	ACGT	5 cut(s)	1294, 2114, 2692, 2745, 2965
KpnI	GGTACC	1 cut(s)	852
Ksp22I	TGATCA	3 cut(s)	355, 984, 1948
Kzo9I	GATC	8 cut(s)	72, 355, 637, 725, 790, 984, 1510, 1948
LguI	GCTCTTC	1 cut(s)	2412
LmnI	GCTCC	4 cut(s)	1471, 1623, 2383, 2467
LweI	GCATC	6 cut(s)	985, 1195, 1425, 1565, 1587, 3000
MaeI	CTAG	6 cut(s)	732, 917, 932, 1538, 1658, 2835
MaeII	ACGT	5 cut(s)	1294, 2114, 2692, 2745, 2965
MaeIII	GTNAC	7 cut(s)	188, 654, 1130, 1451, 1989, 2346, 2625
MalI	GATC	8 cut(s)	74, 357, 639, 727, 792, 986, 1512, 1950
MboI	GATC	8 cut(s)	72, 355, 637, 725, 790, 984, 1510, 1948
MfeI	CAATTG	4 cut(s)	1480, 2098, 2607, 2788
MflI	RGATCY	2 cut(s)	637, 1510
MhlI	GDGCHC	7 cut(s)	247, 488, 557, 739, 1620, 2028, 2407
MlyI	GAGTC	9 cut(s)	266, 1038, 1256, 1373, 2201, 2229, 2327, 2366, 2905
Mph1103I	ATGCAT	2 cut(s)	1580, 2529
MslI	CAYNNNNRTG	2 cut(s)	1407, 1699
MspA1I	CMGCKG	1 cut(s)	2185
MspCI	CTTAAG	2 cut(s)	1394, 2333
MspI	CCGG	3 cut(s)	1885, 2553, 2623
MspR9I	CCNGG	5 cut(s)	1019, 1886, 2534, 2554, 2600
MunI	CAATTG	4 cut(s)	1480, 2098, 2607, 2788
MvaI	CCWGG	3 cut(s)	1019, 2534, 2600
MvnI	CGCG	2 cut(s)	183, 234
NciI	CCSGG	2 cut(s)	1886, 2554
NdeI	CATATG	2 cut(s)	660, 806
NdeII	GATC	8 cut(s)	72, 355, 637, 725, 790, 984, 1510, 1948
NlaIV	GGNNCC	8 cut(s)	305, 555, 556, 604, 850, 2385, 2443, 2604
NmeAIII	GCCGAG	3 cut(s)	289, 314, 722
NmuCI	GTSAC	2 cut(s)	188, 1989
NsbI	TGCGCA	1 cut(s)	1082
NsiI	ATGCAT	2 cut(s)	1580, 2529
NspI	RCATGY	6 cut(s)	1459, 2527, 2581, 2707, 2905, 2976
OliI	CACNNNNGTG	1 cut(s)	1407
PaeI	GCATGC	2 cut(s)	2905, 2976
PagI	TCATGA	4 cut(s)	75, 400, 739, 873
PciI	ACATGT	1 cut(s)	2703
PciSI	GCTCTTC	1 cut(s)	2412
PfeI	GAWTC	8 cut(s)	362, 563, 772, 859, 898, 1567, 1739, 1842
PflFI	GACNNNGTC	1 cut(s)	1047
PflMI	CCANNNNNTGG	2 cut(s)	955, 2425
PfoI	TCCNGGA	1 cut(s)	1017
PinAI	ACCGGT	1 cut(s)	2622
PleI	GAGTC	9 cut(s)	265, 1038, 1255, 1372, 2200, 2229, 2326, 2366, 2905
PpsI	GAGTC	9 cut(s)	265, 1038, 1255, 1372, 2200, 2229, 2326, 2366, 2905
Ppu21I	YACGTR	2 cut(s)	1295, 2966
PscI	ACATGT	1 cut(s)	2703
PshBI	ATTAAT	1 cut(s)	687
Psp124BI	GAGCTC	1 cut(s)	488
Psp6I	CCWGG	3 cut(s)	1017, 2532, 2598
PspGI	CCWGG	3 cut(s)	1017, 2532, 2598
PspN4I	GGNNCC	8 cut(s)	305, 555, 556, 604, 850, 2385, 2443, 2604
PspOMI	GGGCCC	1 cut(s)	553
PspPI	GGNCC	5 cut(s)	291, 553, 554, 679, 1723
PstI	CTGCAG	5 cut(s)	343, 460, 716, 1850, 2634
PstNI	CAGNNNCTG	1 cut(s)	1208
PsuI	RGATCY	2 cut(s)	637, 1510
PsyI	GACNNNGTC	1 cut(s)	1047
RsaI	GTAC	5 cut(s)	850, 1542, 2363, 2485, 2794
RsaNI	GTAC	5 cut(s)	849, 1541, 2362, 2484, 2793
RseI	CAYNNNNRTG	2 cut(s)	1407, 1699
SacI	GAGCTC	1 cut(s)	488
SapI	GCTCTTC	1 cut(s)	2412
Sau3AI	GATC	8 cut(s)	72, 355, 637, 725, 790, 984, 1510, 1948
Sau96I	GGNCC	5 cut(s)	291, 553, 554, 679, 1723
ScaI	AGTACT	1 cut(s)	2363
SchI	GAGTC	9 cut(s)	266, 1038, 1256, 1373, 2201, 2229, 2327, 2366, 2905
ScrFI	CCNGG	5 cut(s)	1019, 1886, 2534, 2554, 2600
SduI	GDGCHC	7 cut(s)	247, 488, 557, 739, 1620, 2028, 2407
SfaNI	GCATC	6 cut(s)	985, 1195, 1425, 1565, 1587, 3000
SfcI	CTRYAG	7 cut(s)	339, 456, 469, 712, 1846, 1857, 2630
SinI	GGWCC	2 cut(s)	679, 1723
SmiMI	CAYNNNNRTG	2 cut(s)	1407, 1699
SmlI	CTYRAG	6 cut(s)	902, 1361, 1394, 1460, 2057, 2333
SmoI	CTYRAG	6 cut(s)	902, 1361, 1394, 1460, 2057, 2333
SpeI	ACTAGT	1 cut(s)	2834
SphI	GCATGC	2 cut(s)	2905, 2976
SspI	AATATT	3 cut(s)	841, 1531, 2662
SspMI	CTAG	6 cut(s)	732, 917, 932, 1538, 1658, 2835
SstI	GAGCTC	1 cut(s)	488
StyD4I	CCNGG	5 cut(s)	1017, 1884, 2532, 2552, 2598
StyI	CCWWGG	4 cut(s)	31, 916, 954, 2379
TaiI	ACGT	5 cut(s)	1297, 2117, 2695, 2748, 2968
TaqI	TCGA	7 cut(s)	775, 1812, 1840, 1902, 2034, 2316, 2463
TatI	WGTACW	3 cut(s)	1540, 2361, 2792
TauI	GCSGC	3 cut(s)	186, 237, 240
TfiI	GAWTC	8 cut(s)	362, 563, 772, 859, 898, 1567, 1739, 1842
TscAI	CASTG	6 cut(s)	254, 725, 1701, 1777, 2554, 2880
TseFI	GTSAC	2 cut(s)	188, 1989
Tsp45I	GTSAC	2 cut(s)	188, 1989
TspRI	CASTG	6 cut(s)	254, 725, 1701, 1777, 2554, 2880
Tth111I	GACNNNGTC	1 cut(s)	1047
Van91I	CCANNNNNTGG	2 cut(s)	955, 2425
Vha464I	CTTAAG	2 cut(s)	1394, 2333
VpaK11BI	GGWCC	2 cut(s)	679, 1723
VspI	ATTAAT	1 cut(s)	687
XagI	CCTNNNNNAGG	1 cut(s)	298
XapI	RAATTY	8 cut(s)	442, 763, 1642, 1750, 1967, 1996, 2260, 2734
XceI	RCATGY	6 cut(s)	1459, 2527, 2581, 2707, 2905, 2976
XmaJI	CCTAGG	1 cut(s)	916
XmiI	GTMKAC	1 cut(s)	1489
XspI	CTAG	6 cut(s)	732, 917, 932, 1538, 1658, 2835
ZrmI	AGTACT	1 cut(s)	2363
Zsp2I	ATGCAT	2 cut(s)	1580, 2529

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.