pycom08g05910

F-box LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0008606
Physical Location & Seq
Forward (+)
4491675 .. 4493941
2267 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g05910.3

Sequence Viewer

Length: 1179 bp
ATGAAGATTTGGTGCTGCCTATGCTTCACCCAAGGAGACGAACCAGAAGAGGACGATAGCGAGGCGGAGTCGATCATGAAGGAGGGGGATTTCGGAAATGACGGCAATTCGGAGGGCGCCATCGGAAATGGCGACGCAATTGACGGCAATGCTCAGAGTTTGGGGCTGGTGGGGAAAGAGCGCGGCCGTAATGACCATCTGGTATTGTTCGAAGGGATGGTTGAGGCAATGCGTGGTGGTGGCGCTCAGTGGGATGAGTCTGTTTGTGTTGATGCTCTTGCGTCGTTGAGGGCGTCGATTGGGAACCCTTGGATTTCCGAGGGCGAGAGTAGCTCAGCTGCAGACAATGGCAGTGATCACGATTCGCACCATAAGCGAGCCAAAGTTCAATCCTTTAATCATGATTTCCACTATGCAATGGCTATGTCTTCAGGTGCTGGAAATTCCAGTTCATCTGCAGAAAGAGACTACAGAATAAATGAGAGCTCTTTTGTTCCATATAAGAGCGAGACATTTTTTCAAAATACTACTCCTAATAATGGCGGTGAGGAGGGCCCCTTTGATTCTGGCAGTGGTAAGGATGATGAAGGTGATCAGAGTGGCACCTCAAAAACAGAAGATTTAGAAGTTCTGATCGATCTTACAGATGACCTATTACATATGGTTTTCTCTTTCTTGGACCACATTAATCTTTGCCGAGCTGCAATAGTCTGTAGGCAGTGGCGATCTGCTAGTGCTCATGAAGATTTCTGGAGGTGTCTGAATTTTGAGAATCGAAGTATATCTGTGGAGCAATTTGAGGACATATGTTGGCGGTATCCCAATGCCACAGAATTGAATATTTCTGGTACCCCTGCTATTCACTCGCTTGTCATGAAAGCTCTCTCTTCATTGAGGAATCTCGAGGTTTTAATCCTAGGGAAAGGGCAACTAGGGGACATCTTTTTCCATTCCTTGGCAGACTGTCAAATGTTGAAAAGTTTGATCATCAATGATGCTACTCTTGGTAATGGTATCCAGGAGATACCTATAAACCATGATAGACTGCGTCATCTTCAATTAACAAAATGTCGTGTTATGCGGATATCCATCAGGTGTCCTCAACTTGAAACTCTGTCATTGAAACGCAGTAACATGGCACAGGCTGTTCTTAATAGCCCTCTTTTGCATCGATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009653 GO:0010252 GO:0032502 GO:0042592 GO:0048646 GO:0048856 GO:0048878 GO:0065007 GO:0065008 GO:1905393
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like

Protein Analysis

393

Amino Acids

43.21

Weight (kDa)

4.87

Isoelectric Point (pI)

42.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 848
AccB1I GGYRCC 3 cut(s) 116, 602, 848
AccB7I CCANNNNNTGG 1 cut(s) 955
AccII CGCG 1 cut(s) 183
AciI CCGC 5 cut(s) 65, 183, 543, 814, 1081
AcoI YGGCCR 1 cut(s) 184
AcsI RAATTY 2 cut(s) 442, 763
AcuI CTGAAG 1 cut(s) 414
AcyI GRCGYC 2 cut(s) 117, 293
AfaI GTAC 1 cut(s) 850
AfiI CCNNNNNNNGG 2 cut(s) 539, 955
AgsI TTSAA 7 cut(s) 389, 521, 838, 976, 1058, 1109, 1123
AjnI CCWGG 1 cut(s) 1017
AluBI AGCT 5 cut(s) 333, 338, 486, 701, 881
AluI AGCT 5 cut(s) 333, 338, 486, 701, 881
Alw21I GWGCWC 2 cut(s) 488, 739
Alw26I GTCTC 3 cut(s) 30, 459, 503
AlwNI CAGNNNCTG 1 cut(s) 437
Ama87I CYCGRG 1 cut(s) 902
AoxI GGCC 2 cut(s) 184, 553
ApaI GGGCCC 1 cut(s) 557
ApeKI GCWGC 3 cut(s) 15, 338, 701
ApoI RAATTY 2 cut(s) 442, 763
AseI ATTAAT 1 cut(s) 687
Asp718I GGTACC 1 cut(s) 848
AspA2I CCTAGG 1 cut(s) 916
AspLEI GCGC 3 cut(s) 119, 183, 245
AspS9I GGNCC 3 cut(s) 553, 554, 679
AsuHPI GGTGA 3 cut(s) 19, 557, 602
AsuII TTCGAA 1 cut(s) 210
AvaI CYCGRG 1 cut(s) 902
AvaII GGWCC 1 cut(s) 679
AvrII CCTAGG 1 cut(s) 916
BaeGI GKGCMC 1 cut(s) 557
BanI GGYRCC 3 cut(s) 116, 602, 848
BanII GRGCYC 2 cut(s) 488, 557
BbsI GAAGAC 1 cut(s) 420
Bbv12I GWGCWC 2 cut(s) 488, 739
BbvI GCAGC 3 cut(s) 2, 325, 688
BccI CCATC 4 cut(s) 128, 204, 211, 1097
BceAI ACGGC 3 cut(s) 118, 160, 171
BciT130I CCWGG 1 cut(s) 1019
BciVI GTATCC 2 cut(s) 828, 1025
BclI TGATCA 3 cut(s) 355, 592, 984
BcoDI GTCTC 3 cut(s) 30, 459, 503
BfaI CTAG 3 cut(s) 732, 917, 932
BfmI CTRYAG 4 cut(s) 339, 456, 469, 712
BfoI RGCGCY 2 cut(s) 120, 246
BfuI GTATCC 2 cut(s) 828, 1025
BisI GCNGC 4 cut(s) 16, 184, 339, 702
BlnI CCTAGG 1 cut(s) 916
BlpI GCTNAGC 1 cut(s) 334
BlsI GCNGC 4 cut(s) 17, 185, 340, 703
Bme1390I CCNGG 1 cut(s) 1019
Bme18I GGWCC 1 cut(s) 679
BmeT110I CYCGRG 1 cut(s) 902
BmgT120I GGNCC 3 cut(s) 553, 554, 679
BmiI GGNNCC 6 cut(s) 118, 305, 555, 556, 604, 850
BmrFI CCNGG 1 cut(s) 1019
BmsI GCATC 2 cut(s) 262, 985
BpiI GAAGAC 1 cut(s) 420
BplI GAGNNNNNCTC 2 cut(s) 317, 349
BpmI CTGGAG 1 cut(s) 772
Bpu1102I GCTNAGC 1 cut(s) 334
Bpu14I TTCGAA 1 cut(s) 210
Bsa29I ATCGAT 2 cut(s) 636, 1171
BsaBI GATNNNNATC 1 cut(s) 1088
BsaHI GRCGYC 2 cut(s) 117, 293
BsaJI CCNNGG 5 cut(s) 31, 308, 318, 916, 954
Bsc4I CCNNNNNNNGG 2 cut(s) 539, 955
Bse1I ACTGG 1 cut(s) 447
Bse3DI GCAATG 3 cut(s) 154, 234, 423
Bse8I GATNNNNATC 1 cut(s) 1088
BseBI CCWGG 1 cut(s) 1019
BseCI ATCGAT 2 cut(s) 636, 1171
BseDI CCNNGG 5 cut(s) 31, 308, 318, 916, 954
BseGI GGATG 3 cut(s) 222, 259, 586
BseJI GATNNNNATC 1 cut(s) 1088
BseLI CCNNNNNNNGG 2 cut(s) 539, 955
BseMI GCAATG 3 cut(s) 154, 234, 423
BseMII CTCAG 3 cut(s) 167, 260, 348
BseNI ACTGG 1 cut(s) 447
BseRI GAGGAG 1 cut(s) 563
BseSI GKGCMC 1 cut(s) 557
BseX3I CGGCCG 1 cut(s) 184
BseXI GCAGC 3 cut(s) 2, 325, 688
Bsh1236I CGCG 1 cut(s) 183
Bsh1285I CGRYCG 1 cut(s) 187
BshFI GGCC 2 cut(s) 186, 555
BshNI GGYRCC 3 cut(s) 116, 602, 848
BshVI ATCGAT 2 cut(s) 636, 1171
BsiEI CGRYCG 1 cut(s) 187
BsiHKAI GWGCWC 2 cut(s) 488, 739
BsiHKCI CYCGRG 1 cut(s) 902
BslFI GGGAC 1 cut(s) 950
BslI CCNNNNNNNGG 2 cut(s) 539, 955
BsmAI GTCTC 3 cut(s) 30, 459, 503
BsmBI CGTCTC 1 cut(s) 30
BsmFI GGGAC 1 cut(s) 950
BsnI GGCC 2 cut(s) 186, 555
BsoBI CYCGRG 1 cut(s) 902
Bsp119I TTCGAA 1 cut(s) 210
Bsp120I GGGCCC 1 cut(s) 553
Bsp1286I GDGCHC 3 cut(s) 488, 557, 739
Bsp143I GATC 8 cut(s) 72, 355, 592, 633, 637, 725, 984, 1172
Bsp1720I GCTNAGC 1 cut(s) 334
BspACI CCGC 5 cut(s) 65, 183, 543, 814, 1081
BspANI GGCC 2 cut(s) 186, 555
BspCNI CTCAG 3 cut(s) 166, 259, 347
BspDI ATCGAT 2 cut(s) 636, 1171
BspFNI CGCG 1 cut(s) 183
BspHI TCATGA 4 cut(s) 75, 400, 739, 873
BspLI GGNNCC 6 cut(s) 118, 305, 555, 556, 604, 850
BspMAI CTGCAG 2 cut(s) 343, 460
BspT104I TTCGAA 1 cut(s) 210
BspT107I GGYRCC 3 cut(s) 116, 602, 848
BsrDI GCAATG 3 cut(s) 154, 234, 423
BsrI ACTGG 1 cut(s) 447
BssECI CCNNGG 5 cut(s) 31, 308, 318, 916, 954
BssMI GATC 8 cut(s) 72, 355, 592, 633, 637, 725, 984, 1172
BssNI GRCGYC 2 cut(s) 117, 293
BssT1I CCWWGG 4 cut(s) 31, 308, 916, 954
Bst2UI CCWGG 1 cut(s) 1019
Bst4CI ACNGT 1 cut(s) 965
Bst6I CTCTTC 2 cut(s) 42, 892
BstACI GRCGYC 2 cut(s) 117, 293
BstBI TTCGAA 1 cut(s) 210
BstC8I GCNNGC 1 cut(s) 378
BstDEI CTNAG 3 cut(s) 153, 246, 334
BstF5I GGATG 3 cut(s) 222, 259, 586
BstFNI CGCG 1 cut(s) 183
BstH2I RGCGCY 2 cut(s) 120, 246
BstHHI GCGC 3 cut(s) 119, 183, 245
BstKTI GATC 8 cut(s) 75, 358, 595, 636, 640, 728, 987, 1175
BstMAI GTCTC 3 cut(s) 30, 459, 503
BstMBI GATC 8 cut(s) 72, 355, 592, 633, 637, 725, 984, 1172
BstMCI CGRYCG 1 cut(s) 187
BstMWI GCNNNNNNNGC 3 cut(s) 21, 330, 373
BstNI CCWGG 1 cut(s) 1019
BstSCI CCNGG 1 cut(s) 1017
BstSFI CTRYAG 4 cut(s) 339, 456, 469, 712
BstSLI GKGCMC 1 cut(s) 557
BstUI CGCG 1 cut(s) 183
BstV1I GCAGC 3 cut(s) 2, 325, 688
BstV2I GAAGAC 1 cut(s) 420
BstZI CGGCCG 1 cut(s) 184
Bsu15I ATCGAT 2 cut(s) 636, 1171
BsuI GTATCC 2 cut(s) 828, 1025
BsuRI GGCC 2 cut(s) 186, 555
BsuTUI ATCGAT 2 cut(s) 636, 1171
BtsCI GGATG 3 cut(s) 222, 259, 586
BtsI GCAGTG 3 cut(s) 358, 577, 725
BtsIMutI CAGTG 4 cut(s) 254, 358, 577, 725
Cac8I GCNNGC 1 cut(s) 378
CaiI CAGNNNCTG 1 cut(s) 437
CciI TCATGA 4 cut(s) 75, 400, 739, 873
CfoI GCGC 3 cut(s) 119, 183, 245
Cfr13I GGNCC 3 cut(s) 553, 554, 679
ClaI ATCGAT 2 cut(s) 636, 1171
CseI GACGC 4 cut(s) 143, 270, 282, 1037
Csp6I GTAC 1 cut(s) 849
CviAII CATG 6 cut(s) 76, 401, 740, 874, 1037, 1135
CviQI GTAC 1 cut(s) 849
DdeI CTNAG 3 cut(s) 153, 246, 334
DinI GGCGCC 1 cut(s) 118
DpnI GATC 8 cut(s) 74, 357, 594, 635, 639, 727, 986, 1174
DpnII GATC 8 cut(s) 72, 355, 592, 633, 637, 725, 984, 1172
EaeI YGGCCR 1 cut(s) 184
EagI CGGCCG 1 cut(s) 184
Eam1104I CTCTTC 2 cut(s) 42, 892
EarI CTCTTC 2 cut(s) 42, 892
EciI GGCGGA 1 cut(s) 80
Ecl136II GAGCTC 1 cut(s) 486
EclXI CGGCCG 1 cut(s) 184
Eco130I CCWWGG 4 cut(s) 31, 308, 916, 954
Eco24I GRGCYC 2 cut(s) 488, 557
Eco32I GATATC 1 cut(s) 1086
Eco47I GGWCC 1 cut(s) 679
Eco52I CGGCCG 1 cut(s) 184
Eco53kI GAGCTC 1 cut(s) 486
Eco57I CTGAAG 1 cut(s) 414
Eco88I CYCGRG 1 cut(s) 902
EcoICRI GAGCTC 1 cut(s) 486
EcoO109I RGGNCCY 2 cut(s) 553, 554
EcoRII CCWGG 1 cut(s) 1017
EcoRV GATATC 1 cut(s) 1086
EcoT14I CCWWGG 4 cut(s) 31, 308, 916, 954
EcoT38I GRGCYC 2 cut(s) 488, 557
EgeI GGCGCC 1 cut(s) 118
EheI GGCGCC 1 cut(s) 118
ErhI CCWWGG 4 cut(s) 31, 308, 916, 954
Esp3I CGTCTC 1 cut(s) 30
FaeI CATG 6 cut(s) 79, 404, 743, 877, 1040, 1138
FaqI GGGAC 1 cut(s) 950
FatI CATG 6 cut(s) 75, 400, 739, 873, 1036, 1134
FauNDI CATATG 2 cut(s) 660, 806
FbaI TGATCA 3 cut(s) 355, 592, 984
Fnu4HI GCNGC 4 cut(s) 16, 184, 339, 702
FokI GGATG 3 cut(s) 229, 266, 593
FriOI GRGCYC 2 cut(s) 488, 557
Fsp4HI GCNGC 4 cut(s) 16, 184, 339, 702
FspBI CTAG 3 cut(s) 732, 917, 932
GlaI GCGC 3 cut(s) 118, 182, 244
GluI GCNGC 4 cut(s) 16, 184, 339, 702
GsuI CTGGAG 1 cut(s) 772
HaeII RGCGCY 2 cut(s) 120, 246
HaeIII GGCC 2 cut(s) 186, 555
HgaI GACGC 4 cut(s) 143, 270, 282, 1037
HhaI GCGC 3 cut(s) 119, 183, 245
Hin1I GRCGYC 2 cut(s) 117, 293
Hin1II CATG 6 cut(s) 79, 404, 743, 877, 1040, 1138
Hin6I GCGC 3 cut(s) 117, 181, 243
HinP1I GCGC 3 cut(s) 117, 181, 243
HinfI GANTC 6 cut(s) 68, 257, 362, 563, 772, 898
HphI GGTGA 3 cut(s) 19, 557, 602
Hpy188I TCNGA 8 cut(s) 95, 112, 125, 156, 319, 597, 633, 762
Hpy188III TCNNGA 8 cut(s) 76, 359, 401, 740, 751, 874, 902, 1176
Hpy99I CGWCG 3 cut(s) 137, 286, 298
HpyAV CCTTC 3 cut(s) 73, 206, 581
HpyCH4III ACNGT 1 cut(s) 965
HpyCH4V TGCA 5 cut(s) 341, 416, 458, 704, 1168
HpyF10VI GCNNNNNNNGC 3 cut(s) 21, 330, 373
HpyF3I CTNAG 3 cut(s) 153, 246, 334
Hsp92I GRCGYC 2 cut(s) 117, 293
Hsp92II CATG 6 cut(s) 79, 404, 743, 877, 1040, 1138
HspAI GCGC 3 cut(s) 117, 181, 243
KasI GGCGCC 1 cut(s) 116
KpnI GGTACC 1 cut(s) 852
Ksp22I TGATCA 3 cut(s) 355, 592, 984
Kzo9I GATC 8 cut(s) 72, 355, 592, 633, 637, 725, 984, 1172
LmnI GCTCC 1 cut(s) 790
Lsp1109I GCAGC 3 cut(s) 2, 325, 688
LweI GCATC 2 cut(s) 262, 985
MaeI CTAG 3 cut(s) 732, 917, 932
MaeIII GTNAC 1 cut(s) 1130
MalI GATC 8 cut(s) 74, 357, 594, 635, 639, 727, 986, 1174
MboI GATC 8 cut(s) 72, 355, 592, 633, 637, 725, 984, 1172
MboII GAAGA 7 cut(s) 16, 59, 420, 629, 755, 879, 1046
MfeI CAATTG 1 cut(s) 138
MhlI GDGCHC 3 cut(s) 488, 557, 739
MluCI AATT 7 cut(s) 106, 138, 442, 763, 794, 833, 1058
Mly113I GGCGCC 1 cut(s) 117
MlyI GAGTC 2 cut(s) 77, 266
MseI TTAA 5 cut(s) 396, 687, 911, 1061, 1152
MspA1I CMGCKG 1 cut(s) 338
MspR9I CCNGG 1 cut(s) 1019
MunI CAATTG 1 cut(s) 138
MvaI CCWGG 1 cut(s) 1019
MvnI CGCG 1 cut(s) 183
MwoI GCNNNNNNNGC 3 cut(s) 21, 330, 373
NarI GGCGCC 1 cut(s) 117
NdeI CATATG 2 cut(s) 660, 806
NdeII GATC 8 cut(s) 72, 355, 592, 633, 637, 725, 984, 1172
NlaIII CATG 6 cut(s) 79, 404, 743, 877, 1040, 1138
NlaIV GGNNCC 6 cut(s) 118, 305, 555, 556, 604, 850
NmeAIII GCCGAG 1 cut(s) 722
NspV TTCGAA 1 cut(s) 210
PaeR7I CTCGAG 1 cut(s) 902
PagI TCATGA 4 cut(s) 75, 400, 739, 873
PcsI WCGNNNNNNNCGW 2 cut(s) 129, 290
PfeI GAWTC 4 cut(s) 362, 563, 772, 898
PflFI GACNNNGTC 1 cut(s) 1047
PflMI CCANNNNNTGG 1 cut(s) 955
PfoI TCCNGGA 1 cut(s) 1017
PkrI GCNGC 4 cut(s) 17, 185, 340, 703
PleI GAGTC 2 cut(s) 76, 265
PluTI GGCGCC 1 cut(s) 120
PpsI GAGTC 2 cut(s) 76, 265
PshBI ATTAAT 1 cut(s) 687
Psp124BI GAGCTC 1 cut(s) 488
Psp6I CCWGG 1 cut(s) 1017
PspGI CCWGG 1 cut(s) 1017
PspN4I GGNNCC 6 cut(s) 118, 305, 555, 556, 604, 850
PspOMI GGGCCC 1 cut(s) 553
PspPI GGNCC 3 cut(s) 553, 554, 679
PstI CTGCAG 2 cut(s) 343, 460
PstNI CAGNNNCTG 1 cut(s) 437
PsyI GACNNNGTC 1 cut(s) 1047
PvuII CAGCTG 1 cut(s) 338
RsaI GTAC 1 cut(s) 850
RsaNI GTAC 1 cut(s) 849
SacI GAGCTC 1 cut(s) 488
SaqAI TTAA 5 cut(s) 396, 687, 911, 1061, 1152
SatI GCNGC 4 cut(s) 16, 184, 339, 702
Sau3AI GATC 8 cut(s) 72, 355, 592, 633, 637, 725, 984, 1172
Sau96I GGNCC 3 cut(s) 553, 554, 679
SchI GAGTC 2 cut(s) 77, 266
ScrFI CCNGG 1 cut(s) 1019
SduI GDGCHC 3 cut(s) 488, 557, 739
SfaNI GCATC 2 cut(s) 262, 985
SfcI CTRYAG 4 cut(s) 339, 456, 469, 712
SfoI GGCGCC 1 cut(s) 118
Sfr274I CTCGAG 1 cut(s) 902
SfuI TTCGAA 1 cut(s) 210
SinI GGWCC 1 cut(s) 679
SlaI CTCGAG 1 cut(s) 902
SmlI CTYRAG 1 cut(s) 902
SmoI CTYRAG 1 cut(s) 902
Sse9I AATT 7 cut(s) 106, 138, 442, 763, 794, 833, 1058
SsiI CCGC 5 cut(s) 65, 183, 543, 814, 1081
SspDI GGCGCC 1 cut(s) 116
SspI AATATT 1 cut(s) 841
SspMI CTAG 3 cut(s) 732, 917, 932
SstI GAGCTC 1 cut(s) 488
StyD4I CCNGG 1 cut(s) 1017
StyI CCWWGG 4 cut(s) 31, 308, 916, 954
TaaI ACNGT 1 cut(s) 965
TaqI TCGA 7 cut(s) 71, 210, 296, 636, 775, 903, 1171
TasI AATT 7 cut(s) 106, 138, 442, 763, 794, 833, 1058
TauI GCSGC 1 cut(s) 186
TfiI GAWTC 4 cut(s) 362, 563, 772, 898
Tru1I TTAA 5 cut(s) 396, 687, 911, 1061, 1152
Tru9I TTAA 5 cut(s) 396, 687, 911, 1061, 1152
TscAI CASTG 4 cut(s) 254, 358, 577, 725
TseI GCWGC 3 cut(s) 15, 338, 701
TspDTI ATGAA 7 cut(s) 17, 92, 441, 600, 756, 879, 890
TspRI CASTG 4 cut(s) 254, 358, 577, 725
Tth111I GACNNNGTC 1 cut(s) 1047
Van91I CCANNNNNTGG 1 cut(s) 955
VpaK11BI GGWCC 1 cut(s) 679
VspI ATTAAT 1 cut(s) 687
XapI RAATTY 2 cut(s) 442, 763
XhoI CTCGAG 1 cut(s) 902
XmaJI CCTAGG 1 cut(s) 916
XspI CTAG 3 cut(s) 732, 917, 932
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.