pycom08g05920

F-box LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0008606
Physical Location & Seq
Forward (+)
4494021 .. 4497495
3475 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g05920.9

Sequence Viewer

Length: 1758 bp
ATGTCAAATTGTTCATGTGTTACTGATGAAACTCTACGTGAGATTGCTCTTACTTGTGCTAATCTCCATGTTCTCAATGCGTCGTACTGTCCAAATATATCCCTTGAGTCTGTAAGACTGCCAATGTTGACAGTTCTTAAGCTTCACAGTTGTGAGGGCATCACCTCGGCTTCAATGGTTGCAATATCTCATAGTTACATGCTTGAGGTTTTGGAGCTTGACAATTGCAGTCTACTTACCGCTGTGAATTTGGACCTTCCACGCTTGCAGAATATTAGACTAGTACATTGTCGCAAATTTACTGATTTGAATCTACGATGCATCATGCTGTCATCAATAATGGTCTCTAACTGTCCTGTGCTCCACCGTATCAACATCACTTCAAATTCACTTCAGAAACTAGCATTGCAAAAGCAAGAGAGCTTAACCATGCTGGCACTGCAATGCCAAAGTTTGCAAGAAGTGGACCTTACAGATTGTGAATCTTTAACAAATTCTATATGTGATGTTTTCAGCGATGGTGGTGGGTGTCCTTTGCTCAAAACATTAGTTCTTGAAAACTGTGAGAGCTTAACTGCGGTTCGATTCTGTAGCACTTCTTTAGTCAGCCTCTCACTTGTTGGTTGCCGGGCAATTACATCTCTCGAACTGACTTGCCCCTATCTTGAACAAGTTTCTTTAGATGGATGTGATCATCTTGAAAGAGCAGCATTTTGTCCAGTTGGTCTTAAGTCACTAAATTTGGGAATATGTCCCAAATTGAATGCGCTCAGTATCGAAGCGCCAAATATGGTGCTGCTGGAGTTGAAGGGATGTGGTGTGTTGGCTGAAGCATCCATCAATTGTCCACTATTAACATCTCTAGACGCTTCCTTTTGCAGTCAGCTTAGGGACGATTGCTTGTCTGCAACAGCTGCTTCATGCCCGCTGATAGAGTCTTTAATTCTAATGTCATGCCCCTCTGTTGGTTCTGATGGACTCTACTCTTTGCACTGGCTTCCAAATTTGATTCTGCTTGATTTGTCCTACACTTTCTTGACGAACTTGAAGCCAGTTTTCGAGTCTTGTATGAAACTTAAGGTGTTGAAGTTACAAGCCTGCAAGTACTTATCTGACTCATCATTGGAGCCTCTTTACAAGGATGGGGCTCTTCCAGCTCTCCAAGAACTGGATTTGTCTTATGGAACCCTCTGTCAGTCTGCTATCGAGGAGCTTCTTTCTTTTTGTACGCACCTGACTCATGTGAGCTTGAATGGCTGTGTGAACATGCATGACCTGAATTGGGGTAGCAGTGCCGGGCAACCTTCCCTATCTGTCATGTTTTTACCTGAGAATGTCCAGGTGCCAATTGAGCAGCCAAACCGGTTACTGCAGAACCTCAACTGTGTAGGTTGTCCAAATATTAGGAAAGTTGTCATTCCACCAGCAGCACGTTGTTTCCACATGTCATCTTTAAACCTTTCTCTTTCTGCAAATTTAAAGGACGTTGATGTTGCTTGTTTCAACCTCTGCTTTCTTAATTTGAGCAATTGTACTTCTTTGGAAGTTTTGAAGCTTGACTGTCCGAAATTGACTAGTCTCTTTCTTCAGTCTTGCAACATCGATGAAGCAGCGGTGGAAGCTGCCATATCGAAATGTAGCATGCTGGAGACTCTTGATGTTCGTTTTTGTCCCAAGATAAGCCCGATGAGCATGGGGAGATTACGTGCTGCATGCCCTAATTTGAAGCGCATCTTCAGCAGCCTGCCACAGTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009653 GO:0010252 GO:0032502 GO:0042592 GO:0048646 GO:0048856 GO:0048878 GO:0065007 GO:0065008 GO:1905393
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like

Protein Analysis

586

Amino Acids

63.7

Weight (kDa)

5.3

Isoelectric Point (pI)

51.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1342
AccB7I CCANNNNNTGG 1 cut(s) 1168
AccI GTMKAC 1 cut(s) 232
AciI CCGC 4 cut(s) 240, 578, 926, 1613
AcsI RAATTY 7 cut(s) 247, 296, 385, 493, 739, 1003, 1474
AcuI CTGAAG 4 cut(s) 377, 849, 1571, 1720
AfaI GTAC 5 cut(s) 86, 285, 1106, 1228, 1534
AfiI CCNNNNNNNGG 3 cut(s) 965, 1168, 1282
AflII CTTAAG 3 cut(s) 137, 728, 1076
AflIII ACRYGT 1 cut(s) 1443
AgeI ACCGGT 1 cut(s) 1362
AhlI ACTAGT 2 cut(s) 280, 1574
AjnI CCWGG 1 cut(s) 1338
AleI CACNNNNGTG 1 cut(s) 150
Alw21I GWGCWC 1 cut(s) 363
Alw26I GTCTC 3 cut(s) 349, 1583, 1643
ApeKI GCWGC 9 cut(s) 707, 796, 914, 1354, 1427, 1610, 1622, 1709, 1740
ApoI RAATTY 7 cut(s) 247, 296, 385, 493, 739, 1003, 1474
AsiGI ACCGGT 1 cut(s) 1362
AspLEI GCGC 3 cut(s) 769, 784, 1731
AspS9I GGNCC 2 cut(s) 253, 466
AsuC2I CCSGG 2 cut(s) 629, 1297
AsuHPI GGTGA 1 cut(s) 154
AvaII GGWCC 2 cut(s) 253, 466
BanI GGYRCC 1 cut(s) 1342
BanII GRGCYC 1 cut(s) 1150
Bbv12I GWGCWC 1 cut(s) 363
BbvI GCAGC 9 cut(s) 719, 783, 901, 1366, 1439, 1609, 1622, 1696, 1752
BccI CCATC 5 cut(s) 512, 677, 845, 968, 1136
BciT130I CCWGG 1 cut(s) 1340
BclI TGATCA 1 cut(s) 691
BcnI CCSGG 2 cut(s) 629, 1297
BcoDI GTCTC 3 cut(s) 349, 1583, 1643
BcuI ACTAGT 2 cut(s) 280, 1574
BfaI CTAG 4 cut(s) 281, 401, 863, 1575
BfmI CTRYAG 2 cut(s) 589, 1370
BfoI RGCGCY 1 cut(s) 785
BfrI CTTAAG 3 cut(s) 137, 728, 1076
BisI GCNGC 9 cut(s) 708, 797, 915, 1355, 1428, 1611, 1623, 1710, 1741
BlsI GCNGC 9 cut(s) 709, 798, 916, 1356, 1429, 1612, 1624, 1711, 1742
BmcAI AGTACT 1 cut(s) 1106
Bme1390I CCNGG 3 cut(s) 629, 1297, 1340
Bme18I GGWCC 2 cut(s) 253, 466
BmgT120I GGNCC 2 cut(s) 253, 466
BmiI GGNNCC 3 cut(s) 1128, 1186, 1344
BmrFI CCNGG 3 cut(s) 629, 1297, 1340
BmsI GCATC 5 cut(s) 168, 308, 330, 842, 1740
BpmI CTGGAG 2 cut(s) 821, 1667
Bpu10I CCTNAGC 1 cut(s) 887
BpuEI CTTGAG 2 cut(s) 125, 224
BpuMI CCSGG 2 cut(s) 629, 1297
Bsa29I ATCGAT 1 cut(s) 1602
BsaAI YACGTR 2 cut(s) 38, 1706
BsaBI GATNNNNATC 1 cut(s) 309
BsaI GGTCTC 1 cut(s) 349
BsaJI CCNNGG 1 cut(s) 165
BsaWI WCCGGW 1 cut(s) 1362
BsaXI ACNNNNNCTCC 2 cut(s) 1690, 1720
Bsc4I CCNNNNNNNGG 3 cut(s) 965, 1168, 1282
Bse118I RCCGGY 1 cut(s) 1362
Bse1I ACTGG 4 cut(s) 719, 998, 1052, 1173
Bse3DI GCAATG 2 cut(s) 404, 449
Bse8I GATNNNNATC 1 cut(s) 309
BseBI CCWGG 1 cut(s) 1340
BseCI ATCGAT 1 cut(s) 1602
BseDI CCNNGG 1 cut(s) 165
BseGI GGATG 4 cut(s) 692, 818, 833, 1147
BseJI GATNNNNATC 1 cut(s) 309
BseLI CCNNNNNNNGG 3 cut(s) 965, 1168, 1282
BseMI GCAATG 2 cut(s) 404, 449
BseMII CTCAG 2 cut(s) 784, 1320
BseNI ACTGG 4 cut(s) 719, 998, 1052, 1173
BseRI GAGGAG 1 cut(s) 1223
BseXI GCAGC 9 cut(s) 719, 783, 901, 1366, 1439, 1609, 1622, 1696, 1752
BshNI GGYRCC 1 cut(s) 1342
BshTI ACCGGT 1 cut(s) 1362
BshVI ATCGAT 1 cut(s) 1602
BsiHKAI GWGCWC 1 cut(s) 363
BsiSI CCGG 3 cut(s) 628, 1296, 1363
BslFI GGGAC 3 cut(s) 738, 905, 1656
BslI CCNNNNNNNGG 3 cut(s) 965, 1168, 1282
BsmAI GTCTC 3 cut(s) 349, 1583, 1643
BsmFI GGGAC 3 cut(s) 738, 905, 1656
BsmI GAATGC 1 cut(s) 769
Bso31I GGTCTC 1 cut(s) 349
Bsp1286I GDGCHC 2 cut(s) 363, 1150
Bsp143I GATC 1 cut(s) 691
BspACI CCGC 4 cut(s) 240, 578, 926, 1613
BspCNI CTCAG 2 cut(s) 783, 1321
BspDI ATCGAT 1 cut(s) 1602
BspLI GGNNCC 3 cut(s) 1128, 1186, 1344
BspMAI CTGCAG 1 cut(s) 1374
BspQI GCTCTTC 1 cut(s) 1155
BspT107I GGYRCC 1 cut(s) 1342
BspTI CTTAAG 3 cut(s) 137, 728, 1076
BspTNI GGTCTC 1 cut(s) 349
BsrDI GCAATG 2 cut(s) 404, 449
BsrFI RCCGGY 1 cut(s) 1362
BsrI ACTGG 4 cut(s) 719, 998, 1052, 1173
BssAI RCCGGY 1 cut(s) 1362
BssECI CCNNGG 1 cut(s) 165
BssMI GATC 1 cut(s) 691
Bst2UI CCWGG 1 cut(s) 1340
Bst4CI ACNGT 9 cut(s) 89, 133, 149, 353, 368, 563, 1385, 1562, 1752
Bst6I CTCTTC 1 cut(s) 1155
BstAFI CTTAAG 3 cut(s) 137, 728, 1076
BstAPI GCANNNNNTGC 1 cut(s) 914
BstBAI YACGTR 2 cut(s) 38, 1706
BstC8I GCNNGC 7 cut(s) 266, 435, 926, 1099, 1643, 1714, 1745
BstDEI CTNAG 3 cut(s) 770, 887, 1329
BstF5I GGATG 4 cut(s) 692, 818, 833, 1147
BstH2I RGCGCY 1 cut(s) 785
BstHHI GCGC 3 cut(s) 769, 784, 1731
BstKTI GATC 1 cut(s) 694
BstMAI GTCTC 3 cut(s) 349, 1583, 1643
BstMBI GATC 1 cut(s) 691
BstMWI GCNNNNNNNGC 8 cut(s) 439, 914, 1154, 1254, 1351, 1619, 1689, 1737
BstNI CCWGG 1 cut(s) 1340
BstNSI RCATGY 5 cut(s) 202, 1270, 1447, 1645, 1716
BstSCI CCNGG 3 cut(s) 627, 1295, 1338
BstSFI CTRYAG 2 cut(s) 589, 1370
BstV1I GCAGC 9 cut(s) 719, 783, 901, 1366, 1439, 1609, 1622, 1696, 1752
Bsu15I ATCGAT 1 cut(s) 1602
BsuTUI ATCGAT 1 cut(s) 1602
BtgZI GCGATG 1 cut(s) 531
BtsCI GGATG 4 cut(s) 692, 818, 833, 1147
BtsI GCAGTG 2 cut(s) 437, 1297
BtsIMutI CAGTG 3 cut(s) 437, 991, 1297
Cac8I GCNNGC 7 cut(s) 266, 435, 926, 1099, 1643, 1714, 1745
CfoI GCGC 3 cut(s) 769, 784, 1731
Cfr10I RCCGGY 1 cut(s) 1362
Cfr13I GGNCC 2 cut(s) 253, 466
ClaI ATCGAT 1 cut(s) 1602
CseI GACGC 2 cut(s) 69, 875
Csp6I GTAC 5 cut(s) 85, 284, 1105, 1227, 1533
CspAI ACCGGT 1 cut(s) 1362
CviQI GTAC 5 cut(s) 85, 284, 1105, 1227, 1533
DdeI CTNAG 3 cut(s) 770, 887, 1329
DpnI GATC 1 cut(s) 693
DpnII GATC 1 cut(s) 691
DraI TTTAAA 2 cut(s) 1455, 1479
Eam1104I CTCTTC 1 cut(s) 1155
EarI CTCTTC 1 cut(s) 1155
Eco24I GRGCYC 1 cut(s) 1150
Eco31I GGTCTC 1 cut(s) 349
Eco47I GGWCC 2 cut(s) 253, 466
Eco57I CTGAAG 4 cut(s) 377, 849, 1571, 1720
EcoRII CCWGG 1 cut(s) 1338
EcoT22I ATGCAT 2 cut(s) 323, 1272
EcoT38I GRGCYC 1 cut(s) 1150
FalI AAGNNNNNCTT 4 cut(s) 453, 485, 1718, 1750
FaqI GGGAC 3 cut(s) 738, 905, 1656
FauI CCCGC 1 cut(s) 933
FbaI TGATCA 1 cut(s) 691
FblI GTMKAC 1 cut(s) 232
Fnu4HI GCNGC 9 cut(s) 708, 797, 915, 1355, 1428, 1611, 1623, 1710, 1741
FokI GGATG 4 cut(s) 699, 820, 825, 1154
FriOI GRGCYC 1 cut(s) 1150
Fsp4HI GCNGC 9 cut(s) 708, 797, 915, 1355, 1428, 1611, 1623, 1710, 1741
FspBI CTAG 4 cut(s) 281, 401, 863, 1575
GlaI GCGC 3 cut(s) 768, 783, 1730
GluI GCNGC 9 cut(s) 708, 797, 915, 1355, 1428, 1611, 1623, 1710, 1741
GsuI CTGGAG 2 cut(s) 821, 1667
HaeII RGCGCY 1 cut(s) 785
HapII CCGG 3 cut(s) 628, 1296, 1363
HgaI GACGC 2 cut(s) 69, 875
HhaI GCGC 3 cut(s) 769, 784, 1731
Hin6I GCGC 3 cut(s) 767, 782, 1729
HinP1I GCGC 3 cut(s) 767, 782, 1729
HincII GTYRAC 1 cut(s) 129
HindII GTYRAC 1 cut(s) 129
HindIII AAGCTT 2 cut(s) 140, 1553
HpaII CCGG 3 cut(s) 628, 1296, 1363
HphI GGTGA 1 cut(s) 154
Hpy166II GTNNAC 5 cut(s) 129, 233, 466, 848, 1264
Hpy188I TCNGA 4 cut(s) 396, 973, 1114, 1566
Hpy188III TCNNGA 8 cut(s) 554, 644, 665, 698, 863, 1036, 1655, 1755
Hpy8I GTNNAC 5 cut(s) 129, 233, 466, 848, 1264
Hpy99I CGWCG 1 cut(s) 85
HpyAV CCTTC 3 cut(s) 266, 802, 1314
HpyCH4III ACNGT 9 cut(s) 89, 133, 149, 353, 368, 563, 1385, 1562, 1752
HpyCH4IV ACGT 4 cut(s) 37, 1432, 1485, 1705
HpyF10VI GCNNNNNNNGC 8 cut(s) 439, 914, 1154, 1254, 1351, 1619, 1689, 1737
HpyF3I CTNAG 3 cut(s) 770, 887, 1329
HpySE526I ACGT 4 cut(s) 37, 1432, 1485, 1705
HspAI GCGC 3 cut(s) 767, 782, 1729
Ksp22I TGATCA 1 cut(s) 691
Kzo9I GATC 1 cut(s) 691
LguI GCTCTTC 1 cut(s) 1155
LmnI GCTCC 4 cut(s) 214, 366, 1126, 1210
Lsp1109I GCAGC 9 cut(s) 719, 783, 901, 1366, 1439, 1609, 1622, 1696, 1752
LweI GCATC 5 cut(s) 168, 308, 330, 842, 1740
MaeI CTAG 4 cut(s) 281, 401, 863, 1575
MaeII ACGT 4 cut(s) 37, 1432, 1485, 1705
MaeIII GTNAC 5 cut(s) 19, 194, 732, 1089, 1365
MalI GATC 1 cut(s) 693
MboI GATC 1 cut(s) 691
MboII GAAGA 3 cut(s) 1142, 1577, 1726
MfeI CAATTG 4 cut(s) 223, 841, 1347, 1528
MhlI GDGCHC 2 cut(s) 363, 1150
MlyI GAGTC 7 cut(s) 116, 944, 972, 1070, 1109, 1231, 1645
Mph1103I ATGCAT 2 cut(s) 323, 1272
MslI CAYNNNNRTG 2 cut(s) 150, 442
MspA1I CMGCKG 4 cut(s) 242, 914, 928, 1613
MspCI CTTAAG 3 cut(s) 137, 728, 1076
MspI CCGG 3 cut(s) 628, 1296, 1363
MspR9I CCNGG 3 cut(s) 629, 1297, 1340
MunI CAATTG 4 cut(s) 223, 841, 1347, 1528
Mva1269I GAATGC 1 cut(s) 769
MvaI CCWGG 1 cut(s) 1340
MwoI GCNNNNNNNGC 8 cut(s) 439, 914, 1154, 1254, 1351, 1619, 1689, 1737
NciI CCSGG 2 cut(s) 629, 1297
NdeII GATC 1 cut(s) 691
NlaIV GGNNCC 3 cut(s) 1128, 1186, 1344
NmeAIII GCCGAG 1 cut(s) 146
NmuCI GTSAC 1 cut(s) 732
NsiI ATGCAT 2 cut(s) 323, 1272
NspI RCATGY 5 cut(s) 202, 1270, 1447, 1645, 1716
OliI CACNNNNGTG 1 cut(s) 150
PaeI GCATGC 2 cut(s) 1645, 1716
PciI ACATGT 1 cut(s) 1443
PciSI GCTCTTC 1 cut(s) 1155
PctI GAATGC 1 cut(s) 769
PfeI GAWTC 4 cut(s) 310, 482, 585, 1009
PflMI CCANNNNNTGG 1 cut(s) 1168
PinAI ACCGGT 1 cut(s) 1362
PkrI GCNGC 9 cut(s) 709, 798, 916, 1356, 1429, 1612, 1624, 1711, 1742
PleI GAGTC 7 cut(s) 115, 943, 972, 1069, 1109, 1231, 1645
PpsI GAGTC 7 cut(s) 115, 943, 972, 1069, 1109, 1231, 1645
Ppu21I YACGTR 2 cut(s) 38, 1706
PscI ACATGT 1 cut(s) 1443
Psp6I CCWGG 1 cut(s) 1338
PspGI CCWGG 1 cut(s) 1338
PspN4I GGNNCC 3 cut(s) 1128, 1186, 1344
PspPI GGNCC 2 cut(s) 253, 466
PstI CTGCAG 1 cut(s) 1374
PvuII CAGCTG 1 cut(s) 914
RsaI GTAC 5 cut(s) 86, 285, 1106, 1228, 1534
RsaNI GTAC 5 cut(s) 85, 284, 1105, 1227, 1533
RseI CAYNNNNRTG 2 cut(s) 150, 442
SapI GCTCTTC 1 cut(s) 1155
SatI GCNGC 9 cut(s) 708, 797, 915, 1355, 1428, 1611, 1623, 1710, 1741
Sau3AI GATC 1 cut(s) 691
Sau96I GGNCC 2 cut(s) 253, 466
ScaI AGTACT 1 cut(s) 1106
SchI GAGTC 7 cut(s) 116, 944, 972, 1070, 1109, 1231, 1645
ScrFI CCNGG 3 cut(s) 629, 1297, 1340
SduI GDGCHC 2 cut(s) 363, 1150
SfaNI GCATC 5 cut(s) 168, 308, 330, 842, 1740
SfcI CTRYAG 2 cut(s) 589, 1370
SinI GGWCC 2 cut(s) 253, 466
SmiMI CAYNNNNRTG 2 cut(s) 150, 442
SmlI CTYRAG 5 cut(s) 104, 137, 203, 728, 1076
SmoI CTYRAG 5 cut(s) 104, 137, 203, 728, 1076
SpeI ACTAGT 2 cut(s) 280, 1574
SphI GCATGC 2 cut(s) 1645, 1716
SsiI CCGC 4 cut(s) 240, 578, 926, 1613
SspI AATATT 2 cut(s) 274, 1402
SspMI CTAG 4 cut(s) 281, 401, 863, 1575
StyD4I CCNGG 3 cut(s) 627, 1295, 1338
TaaI ACNGT 9 cut(s) 89, 133, 149, 353, 368, 563, 1385, 1562, 1752
TaiI ACGT 4 cut(s) 40, 1435, 1488, 1708
TaqI TCGA 7 cut(s) 583, 645, 777, 1059, 1206, 1602, 1631
TatI WGTACW 3 cut(s) 283, 1104, 1532
TfiI GAWTC 4 cut(s) 310, 482, 585, 1009
TscAI CASTG 3 cut(s) 444, 998, 1297
TseFI GTSAC 1 cut(s) 732
TseI GCWGC 9 cut(s) 707, 796, 914, 1354, 1427, 1610, 1622, 1709, 1740
Tsp45I GTSAC 1 cut(s) 732
TspDTI ATGAA 4 cut(s) 42, 909, 1085, 1620
TspRI CASTG 3 cut(s) 444, 998, 1297
Van91I CCANNNNNTGG 1 cut(s) 1168
Vha464I CTTAAG 3 cut(s) 137, 728, 1076
VpaK11BI GGWCC 2 cut(s) 253, 466
XapI RAATTY 7 cut(s) 247, 296, 385, 493, 739, 1003, 1474
XbaI TCTAGA 1 cut(s) 862
XceI RCATGY 5 cut(s) 202, 1270, 1447, 1645, 1716
XmiI GTMKAC 1 cut(s) 232
XspI CTAG 4 cut(s) 281, 401, 863, 1575
ZrmI AGTACT 1 cut(s) 1106
Zsp2I ATGCAT 2 cut(s) 323, 1272
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.