MD15G1056700.v1.1

Acts as a Mg(2 ) transporter. Can also transport other divalent cations such as Fe(2 ), Sr(2 ), Ba(2 ), Mn(2 ) and Co(2 ) but to a much less extent than Mg(2 )

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0016033
Physical Location & Seq
Forward (+)
3807750 .. 3810673
2924 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1056700.v1.1.491

Sequence Viewer

Length: 1023 bp
ATGTGGGAGTCGATCTTCCTAACGCTGGCGGCGACGGCCGGAAACAACATCGGCAAAATTCTCCAGAAGAAGGGCACCGTCATTCTTCCTCCTCTCTCTTTCAAGCTCAAGGTGATCAGGGCGTATGCGTTGAACAAAGCATGGCTGATAGGATTTATGATGGATATATTCGGAGCTTTGTTAATGTTGAGAGCATTGTCTTTAGCTCCTGTGTCTGTCATACAACCGGTTTCCGGGTGTGGACTTGCAATTCTTTCAATCTTTTCCCATTTTTATCTGAAGGAAATGATGAATGCTGTTGACTGGATGGGGATTACATTAGCAGGCATTGGCACTATAGGGGTTGGTGCCGGAGGTGAGGAGCAAAAGGCTTCCACAATATCGGTGTTTCATCTACCATGGTTGGCAATTGTAGTCGCCTTCTTGTTTGTCCTCCTTAATGGGTGGCTACGCATCTACAGACGTCAACGCAAAGAACAGGAGTTGATGGAATATGAAGTTGTTGAAGAAATTATATATGGCTTGGAATCTGGCATTTTGTTCGGGATGGCATCTGTGATATCAAAGATGGGATTTGTGTTCTTGGAGCAGGGTTTCCACAGCATGCTGGTCCCGATATGTGTGATAATCAGCATATGTTGTAGTGGTACAGGGTTTTATTACCAGACTTGGGGCTTAAAGCATGGGAGGGCAATAGTAGTGTCTACATGTGCTGCTGTGGCATCAATTGTGACTGGTGTACTAGCTGGAATGCTTGCTTTGGGTGAAAGATTGCCTTCAGCACCAACAGCTCGTCTTTCACTGATGCTTGGATGGTTACTCATAATTATTGGTGTGATTTTACTTGTGAGCTCAACACGACTGATGCGACGCCTTCCTCGACCATTACGACAACTTTTAAAGAGTGGTGTTGATCAGAGGAAACCCGGGTCTATCCGTGTTAGGGATGGAAGCCCGAGCACCGTTATTCAGGCAGCAACTCTGCATCATTTGATACCAACTGCTTCAAAAGAGAAGGCTTGA

Protein Analysis

341

Amino Acids

37.06

Weight (kDa)

9.81

Isoelectric Point (pI)

33.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mg_trans_NIPA PF05653 40 - 283 3.6e-06 Magnesium transporter NIPA
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016033)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G11960
fragaria_vesca FvH4_2g31600
malus_domestica MD08G1064900.v1.1 MD15G1056700.v1.1
prunus_persica Prupe.1G409300_v2.0.a1
pyrus_communis pycom08g05320 pycom15g05310
rosa_chinensis RchiOBHm_Chr6g0311221
rosa_laevigata RLG00000010426
rosa_roxburghii Rroxscaffold_7G00157890
rosa_rugosa Rorug06G0386600.1
rosa_samantha Rh6AG500700 Rh6BG511300 Rh6DG503600
rosa_wichuraiana Rw6G043600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 466
AccB1I GGYRCC 2 cut(s) 74, 347
AccI GTMKAC 1 cut(s) 704
AciI CCGC 1 cut(s) 29
AcoI YGGCCR 1 cut(s) 36
AcsI RAATTY 1 cut(s) 57
AcuI CTGAAG 2 cut(s) 299, 762
AcyI GRCGYC 2 cut(s) 463, 871
AfaI GTAC 2 cut(s) 649, 741
AfiI CCNNNNNNNGG 5 cut(s) 25, 70, 233, 670, 943
AflIII ACRYGT 1 cut(s) 707
AgeI ACCGGT 1 cut(s) 226
AgsI TTSAA 5 cut(s) 103, 133, 258, 506, 1008
AluBI AGCT 6 cut(s) 106, 176, 206, 746, 791, 852
AluI AGCT 6 cut(s) 106, 176, 206, 746, 791, 852
Alw21I GWGCWC 2 cut(s) 854, 962
Ama87I CYCGRG 2 cut(s) 926, 955
AoxI GGCC 1 cut(s) 36
ApeKI GCWGC 2 cut(s) 713, 974
ApoI RAATTY 1 cut(s) 57
AsiGI ACCGGT 1 cut(s) 226
AspS9I GGNCC 1 cut(s) 610
AsuC2I CCSGG 3 cut(s) 235, 927, 928
AsuHPI GGTGA 3 cut(s) 124, 368, 776
AvaI CYCGRG 2 cut(s) 926, 955
AvaII GGWCC 1 cut(s) 610
BaeGI GKGCMC 1 cut(s) 77
BanI GGYRCC 2 cut(s) 74, 347
BanII GRGCYC 1 cut(s) 854
Bbv12I GWGCWC 2 cut(s) 854, 962
BbvI GCAGC 2 cut(s) 700, 986
BccI CCATC 7 cut(s) 154, 301, 481, 541, 562, 807, 941
BceAI ACGGC 1 cut(s) 51
BcgI CGANNNNNNTGC 2 cut(s) 523, 557
BclI TGATCA 2 cut(s) 114, 913
BcnI CCSGG 3 cut(s) 235, 927, 928
BfaI CTAG 1 cut(s) 743
BfmI CTRYAG 2 cut(s) 336, 457
BisI GCNGC 3 cut(s) 30, 714, 975
BlsI GCNGC 3 cut(s) 31, 715, 976
Bme1390I CCNGG 3 cut(s) 235, 927, 928
Bme18I GGWCC 1 cut(s) 610
BmeT110I CYCGRG 2 cut(s) 926, 955
BmgT120I GGNCC 1 cut(s) 610
BmiI GGNNCC 3 cut(s) 76, 349, 612
BmrFI CCNGG 3 cut(s) 235, 927, 928
BmsI GCATC 6 cut(s) 462, 560, 731, 795, 855, 994
BpmI CTGGAG 1 cut(s) 47
BpuEI CTTGAG 1 cut(s) 92
BpuMI CCSGG 3 cut(s) 235, 927, 928
BsaHI GRCGYC 2 cut(s) 463, 871
BsaJI CCNNGG 2 cut(s) 398, 926
BsaWI WCCGGW 1 cut(s) 226
Bsc4I CCNNNNNNNGG 5 cut(s) 25, 70, 233, 670, 943
Bse118I RCCGGY 1 cut(s) 226
Bse1I ACTGG 2 cut(s) 308, 739
BseDI CCNNGG 2 cut(s) 398, 926
BseGI GGATG 4 cut(s) 312, 552, 818, 952
BseLI CCNNNNNNNGG 5 cut(s) 25, 70, 233, 670, 943
BseNI ACTGG 2 cut(s) 308, 739
BseRI GAGGAG 2 cut(s) 81, 374
BseSI GKGCMC 1 cut(s) 77
BseX3I CGGCCG 1 cut(s) 36
BseXI GCAGC 2 cut(s) 700, 986
Bsh1285I CGRYCG 1 cut(s) 39
BshFI GGCC 1 cut(s) 38
BshNI GGYRCC 2 cut(s) 74, 347
BshTI ACCGGT 1 cut(s) 226
BsiEI CGRYCG 1 cut(s) 39
BsiHKAI GWGCWC 2 cut(s) 854, 962
BsiHKCI CYCGRG 2 cut(s) 926, 955
BsiSI CCGG 5 cut(s) 39, 227, 234, 351, 927
BslFI GGGAC 1 cut(s) 596
BslI CCNNNNNNNGG 5 cut(s) 25, 70, 233, 670, 943
BsmFI GGGAC 1 cut(s) 596
BsmI GAATGC 2 cut(s) 298, 756
BsnI GGCC 1 cut(s) 38
BsoBI CYCGRG 2 cut(s) 926, 955
Bsp1286I GDGCHC 3 cut(s) 77, 854, 962
Bsp143I GATC 3 cut(s) 12, 114, 913
Bsp19I CCATGG 1 cut(s) 398
BspACI CCGC 1 cut(s) 29
BspANI GGCC 1 cut(s) 38
BspLI GGNNCC 3 cut(s) 76, 349, 612
BspT107I GGYRCC 2 cut(s) 74, 347
BsrFI RCCGGY 1 cut(s) 226
BsrI ACTGG 2 cut(s) 308, 739
BssAI RCCGGY 1 cut(s) 226
BssECI CCNNGG 2 cut(s) 398, 926
BssMI GATC 3 cut(s) 12, 114, 913
BssNI GRCGYC 2 cut(s) 463, 871
BssT1I CCWWGG 1 cut(s) 398
Bst4CI ACNGT 2 cut(s) 79, 964
BstACI GRCGYC 2 cut(s) 463, 871
BstC8I GCNNGC 4 cut(s) 27, 325, 605, 756
BstDSI CCRYGG 1 cut(s) 398
BstF5I GGATG 4 cut(s) 312, 552, 818, 952
BstKTI GATC 3 cut(s) 15, 117, 916
BstMBI GATC 3 cut(s) 12, 114, 913
BstMCI CGRYCG 1 cut(s) 39
BstMWI GCNNNNNNNGC 3 cut(s) 35, 719, 788
BstNSI RCATGY 2 cut(s) 607, 711
BstSCI CCNGG 3 cut(s) 233, 925, 926
BstSFI CTRYAG 2 cut(s) 336, 457
BstSLI GKGCMC 1 cut(s) 77
BstV1I GCAGC 2 cut(s) 700, 986
BstZI CGGCCG 1 cut(s) 36
BsuRI GGCC 1 cut(s) 38
BtgI CCRYGG 1 cut(s) 398
BtsCI GGATG 4 cut(s) 312, 552, 818, 952
BtsIMutI CAGTG 1 cut(s) 800
Cac8I GCNNGC 4 cut(s) 27, 325, 605, 756
Cfr10I RCCGGY 1 cut(s) 226
Cfr13I GGNCC 1 cut(s) 610
Cfr9I CCCGGG 1 cut(s) 926
CseI GACGC 1 cut(s) 879
Csp6I GTAC 2 cut(s) 648, 740
CspAI ACCGGT 1 cut(s) 226
CviAII CATG 5 cut(s) 141, 399, 604, 683, 708
CviQI GTAC 2 cut(s) 648, 740
DpnI GATC 3 cut(s) 14, 116, 915
DpnII GATC 3 cut(s) 12, 114, 913
DraI TTTAAA 1 cut(s) 900
EaeI YGGCCR 1 cut(s) 36
EagI CGGCCG 1 cut(s) 36
Ecl136II GAGCTC 1 cut(s) 852
EclXI CGGCCG 1 cut(s) 36
Eco130I CCWWGG 1 cut(s) 398
Eco24I GRGCYC 1 cut(s) 854
Eco32I GATATC 1 cut(s) 561
Eco47I GGWCC 1 cut(s) 610
Eco52I CGGCCG 1 cut(s) 36
Eco53kI GAGCTC 1 cut(s) 852
Eco57I CTGAAG 2 cut(s) 299, 762
Eco88I CYCGRG 2 cut(s) 926, 955
EcoICRI GAGCTC 1 cut(s) 852
EcoRV GATATC 1 cut(s) 561
EcoT14I CCWWGG 1 cut(s) 398
EcoT38I GRGCYC 1 cut(s) 854
ErhI CCWWGG 1 cut(s) 398
FaeI CATG 5 cut(s) 144, 402, 607, 686, 711
FalI AAGNNNNNCTT 2 cut(s) 760, 792
FaqI GGGAC 1 cut(s) 596
FatI CATG 5 cut(s) 140, 398, 603, 682, 707
FauNDI CATATG 1 cut(s) 635
FbaI TGATCA 2 cut(s) 114, 913
FblI GTMKAC 1 cut(s) 704
Fnu4HI GCNGC 3 cut(s) 30, 714, 975
FokI GGATG 4 cut(s) 319, 559, 825, 959
FriOI GRGCYC 1 cut(s) 854
Fsp4HI GCNGC 3 cut(s) 30, 714, 975
FspBI CTAG 1 cut(s) 743
GluI GCNGC 3 cut(s) 30, 714, 975
GsuI CTGGAG 1 cut(s) 47
HaeIII GGCC 1 cut(s) 38
HapII CCGG 5 cut(s) 39, 227, 234, 351, 927
HgaI GACGC 1 cut(s) 879
Hin1I GRCGYC 2 cut(s) 463, 871
Hin1II CATG 5 cut(s) 144, 402, 607, 686, 711
HincII GTYRAC 2 cut(s) 301, 467
HindII GTYRAC 2 cut(s) 301, 467
HinfI GANTC 2 cut(s) 8, 527
HpaII CCGG 5 cut(s) 39, 227, 234, 351, 927
HphI GGTGA 3 cut(s) 124, 368, 776
Hpy166II GTNNAC 5 cut(s) 242, 301, 467, 705, 740
Hpy188I TCNGA 3 cut(s) 173, 279, 918
Hpy188III TCNNGA 3 cut(s) 64, 544, 613
Hpy8I GTNNAC 5 cut(s) 242, 301, 467, 705, 740
Hpy99I CGWCG 2 cut(s) 37, 873
HpyAV CCTTC 6 cut(s) 64, 274, 430, 786, 884, 1009
HpyCH4III ACNGT 2 cut(s) 79, 964
HpyCH4IV ACGT 1 cut(s) 463
HpyCH4V TGCA 2 cut(s) 248, 985
HpyF10VI GCNNNNNNNGC 3 cut(s) 35, 719, 788
HpySE526I ACGT 1 cut(s) 463
Hsp92I GRCGYC 2 cut(s) 463, 871
Hsp92II CATG 5 cut(s) 144, 402, 607, 686, 711
Ksp22I TGATCA 2 cut(s) 114, 913
Kzo9I GATC 3 cut(s) 12, 114, 913
LmnI GCTCC 4 cut(s) 173, 211, 361, 586
Lsp1109I GCAGC 2 cut(s) 700, 986
LweI GCATC 6 cut(s) 462, 560, 731, 795, 855, 994
MaeI CTAG 1 cut(s) 743
MaeII ACGT 1 cut(s) 463
MaeIII GTNAC 2 cut(s) 730, 816
MalI GATC 3 cut(s) 14, 116, 915
MboI GATC 3 cut(s) 12, 114, 913
MboII GAAGA 4 cut(s) 7, 77, 79, 518
MfeI CAATTG 2 cut(s) 408, 726
MhlI GDGCHC 3 cut(s) 77, 854, 962
MluCI AATT 6 cut(s) 57, 249, 408, 510, 726, 825
MlyI GAGTC 1 cut(s) 17
MnlI CCTC 8 cut(s) 99, 102, 347, 352, 443, 681, 888, 912
MseI TTAA 4 cut(s) 182, 438, 677, 899
MspI CCGG 5 cut(s) 39, 227, 234, 351, 927
MspR9I CCNGG 3 cut(s) 235, 927, 928
MunI CAATTG 2 cut(s) 408, 726
Mva1269I GAATGC 2 cut(s) 298, 756
MwoI GCNNNNNNNGC 3 cut(s) 35, 719, 788
NciI CCSGG 3 cut(s) 235, 927, 928
NcoI CCATGG 1 cut(s) 398
NdeI CATATG 1 cut(s) 635
NdeII GATC 3 cut(s) 12, 114, 913
NlaIII CATG 5 cut(s) 144, 402, 607, 686, 711
NlaIV GGNNCC 3 cut(s) 76, 349, 612
NmuCI GTSAC 1 cut(s) 730
NspI RCATGY 2 cut(s) 607, 711
PaeI GCATGC 1 cut(s) 607
PciI ACATGT 1 cut(s) 707
PcsI WCGNNNNNNNCGW 4 cut(s) 29, 865, 877, 886
PctI GAATGC 2 cut(s) 298, 756
PfeI GAWTC 1 cut(s) 527
PinAI ACCGGT 1 cut(s) 226
PkrI GCNGC 3 cut(s) 31, 715, 976
PleI GAGTC 1 cut(s) 16
PpsI GAGTC 1 cut(s) 16
PscI ACATGT 1 cut(s) 707
Psp124BI GAGCTC 1 cut(s) 854
PspN4I GGNNCC 3 cut(s) 76, 349, 612
PspPI GGNCC 1 cut(s) 610
RsaI GTAC 2 cut(s) 649, 741
RsaNI GTAC 2 cut(s) 648, 740
SacI GAGCTC 1 cut(s) 854
SaqAI TTAA 4 cut(s) 182, 438, 677, 899
SatI GCNGC 3 cut(s) 30, 714, 975
Sau3AI GATC 3 cut(s) 12, 114, 913
Sau96I GGNCC 1 cut(s) 610
SchI GAGTC 1 cut(s) 17
ScrFI CCNGG 3 cut(s) 235, 927, 928
SduI GDGCHC 3 cut(s) 77, 854, 962
SetI ASST 9 cut(s) 108, 114, 178, 208, 358, 466, 748, 793, 854
SfaNI GCATC 6 cut(s) 462, 560, 731, 795, 855, 994
SfcI CTRYAG 2 cut(s) 336, 457
SinI GGWCC 1 cut(s) 610
SmaI CCCGGG 1 cut(s) 928
SmlI CTYRAG 1 cut(s) 107
SmoI CTYRAG 1 cut(s) 107
SphI GCATGC 1 cut(s) 607
Sse9I AATT 6 cut(s) 57, 249, 408, 510, 726, 825
SsiI CCGC 1 cut(s) 29
SspMI CTAG 1 cut(s) 743
SstI GAGCTC 1 cut(s) 854
StyD4I CCNGG 3 cut(s) 233, 925, 926
StyI CCWWGG 1 cut(s) 398
TaaI ACNGT 2 cut(s) 79, 964
TaiI ACGT 1 cut(s) 466
TaqI TCGA 2 cut(s) 11, 880
TasI AATT 6 cut(s) 57, 249, 408, 510, 726, 825
TatI WGTACW 1 cut(s) 739
TauI GCSGC 1 cut(s) 32
TfiI GAWTC 1 cut(s) 527
Tru1I TTAA 4 cut(s) 182, 438, 677, 899
Tru9I TTAA 4 cut(s) 182, 438, 677, 899
TscAI CASTG 1 cut(s) 807
TseFI GTSAC 1 cut(s) 730
TseI GCWGC 2 cut(s) 713, 974
Tsp45I GTSAC 1 cut(s) 730
TspDTI ATGAA 3 cut(s) 305, 380, 510
TspGWI ACGGA 1 cut(s) 926
TspMI CCCGGG 1 cut(s) 926
TspRI CASTG 1 cut(s) 807
VpaK11BI GGWCC 1 cut(s) 610
XapI RAATTY 1 cut(s) 57
XceI RCATGY 2 cut(s) 607, 711
XmaI CCCGGG 1 cut(s) 926
XmiI GTMKAC 1 cut(s) 704
XspI CTAG 1 cut(s) 743
ZraI GACGTC 1 cut(s) 464
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.