Rh6BG511300

Acts as a Mg(2 ) transporter. Can also transport other divalent cations such as Fe(2 ), Sr(2 ), Ba(2 ), Mn(2 ) and Co(2 ) but to a much less extent than Mg(2 )

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0016033
Physical Location & Seq
Reverse (-)
70708787 .. 70711994
3208 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG511300.1

Sequence Viewer

Length: 1020 bp
ATGTGGGAGTCGATCCTTCTGACGTTGGCGGCGACCGCCGGAAACAACATCGGCAAAATTCTTCAGAAGAAGGGCACCGTCATTCTTCCTCCTCTCTCTTTGAAGCTCAAGGTGATAAGGGCATATGCCTTGAACAGAGCTTGGCTGATAGGTTTTCTGATGGATATATTTGGAGCCTTGCTCATGTTGAGAGCACTGTCTCTTGCTCCTGTATCTGTCATCCAGCCGGTTTCTGGGTGTGGACTTGCTATCCTTTCGATATTTTCCCATTTTTATCTGAAGGAAATCATGAATGCTGTTGACTGGCTGGGGATTACTTTAGCAGGCATTGGCACTATAGGAGTTGGTGCTGGAGGTGAGGAGCAAAAGGCTTCTGCTATATCTATCTTCCATCTACCATGGTTGGCAATTGTGGTCGCCATCTTGTTTGTACTCCTTAATGCTTGGCTACGAATCTACAGACGTCAACGAAAAGAACAGGAGTTGATGGAATATGAAGTTGTAGAAGAGATTATATATGGCTTGGAATCTGGCATTTTGTTTGGAATGGCATCTGTAATATCAAAGATGGGCTTTGTGTTCTTGGAGCAGGGTTTCCCCAGCATACTAGTTCCTATATGCTTGATAATCAGCATATGTTGTAGCGGTACAGGGTTTTACTACCAGACTTGGGGTTTAAAGCATGGGAGGGCAATTGTAGTCTCTACATGTGCTGCTGTGGCATCAATTGTTACTGGTGTTGTTGCTGGTATGCTTGCTTTGGGTGAAAGATTGCCTTCAGCGCCAACAGCTCGTCTTACACTTATGCTTGGATGGGTATTTATAATTGGTGGGGTGATTTTACTTGTGAGCTCAACACGGCTGGTGCGTCACCTTCCTCGGTCTTTACGACATGTTGATAAGAGCTTCAACGTTAGAAAATCTGGGTCTACCCGTGTTAGGGAGTCCAACCCTAGCACTGTTATCCAGGCATCAACTCTGCATCATTTGATGCCTACTTCTTCCAAAGAGAAGGCTTGA

Protein Analysis

339

Amino Acids

36.84

Weight (kDa)

9.76

Isoelectric Point (pI)

37.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mg_trans_NIPA PF05653 20 - 283 1.1e-06 Magnesium transporter NIPA
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016033)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G11960
fragaria_vesca FvH4_2g31600
malus_domestica MD08G1064900.v1.1 MD15G1056700.v1.1
prunus_persica Prupe.1G409300_v2.0.a1
pyrus_communis pycom08g05320 pycom15g05310
rosa_chinensis RchiOBHm_Chr6g0311221
rosa_laevigata RLG00000010426
rosa_roxburghii Rroxscaffold_7G00157890
rosa_rugosa Rorug06G0386600.1
rosa_samantha Rh6AG500700 Rh6BG511300 Rh6DG503600
rosa_wichuraiana Rw6G043600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 824
AatII GACGTC 1 cut(s) 466
AccB1I GGYRCC 1 cut(s) 74
AccI GTMKAC 1 cut(s) 929
AciI CCGC 3 cut(s) 29, 36, 645
AclI AACGTT 1 cut(s) 912
AclWI GGATC 1 cut(s) 7
AcsI RAATTY 1 cut(s) 57
AcuI CTGAAG 3 cut(s) 47, 299, 762
AcyI GRCGYC 1 cut(s) 463
AfaI GTAC 2 cut(s) 432, 649
AfiI CCNNNNNNNGG 4 cut(s) 233, 670, 939, 940
AflIII ACRYGT 2 cut(s) 707, 892
AgsI TTSAA 3 cut(s) 103, 133, 910
AhlI ACTAGT 1 cut(s) 607
AjnI CCWGG 1 cut(s) 966
AluBI AGCT 5 cut(s) 106, 140, 791, 852, 906
AluI AGCT 5 cut(s) 106, 140, 791, 852, 906
Alw21I GWGCWC 2 cut(s) 196, 854
Alw26I GTCTC 2 cut(s) 204, 706
AlwI GGATC 1 cut(s) 7
ApeKI GCWGC 1 cut(s) 713
ApoI RAATTY 1 cut(s) 57
AspLEI GCGC 1 cut(s) 784
AsuHPI GGTGA 5 cut(s) 124, 368, 776, 847, 863
BaeGI GKGCMC 1 cut(s) 77
BanI GGYRCC 1 cut(s) 74
BanII GRGCYC 1 cut(s) 854
Bbv12I GWGCWC 2 cut(s) 196, 854
BbvI GCAGC 1 cut(s) 700
BccI CCATC 6 cut(s) 154, 399, 428, 481, 562, 807
BceAI ACGGC 1 cut(s) 875
BciT130I CCWGG 1 cut(s) 968
BcoDI GTCTC 2 cut(s) 204, 706
BcuI ACTAGT 1 cut(s) 607
BfaI CTAG 2 cut(s) 608, 954
BfmI CTRYAG 2 cut(s) 336, 457
BfoI RGCGCY 1 cut(s) 785
BisI GCNGC 2 cut(s) 30, 714
BlsI GCNGC 2 cut(s) 31, 715
Bme1390I CCNGG 1 cut(s) 968
BmiI GGNNCC 2 cut(s) 76, 175
BmrFI CCNGG 1 cut(s) 968
BmsI GCATC 5 cut(s) 560, 731, 980, 981, 991
BplI GAGNNNNNCTC 2 cut(s) 165, 197
BpmI CTGGAG 1 cut(s) 372
BpuEI CTTGAG 1 cut(s) 92
BsaHI GRCGYC 1 cut(s) 463
BsaJI CCNNGG 2 cut(s) 398, 878
Bsc4I CCNNNNNNNGG 4 cut(s) 233, 670, 939, 940
Bse118I RCCGGY 1 cut(s) 226
Bse1I ACTGG 2 cut(s) 308, 739
BseBI CCWGG 1 cut(s) 968
BseDI CCNNGG 2 cut(s) 398, 878
BseGI GGATG 2 cut(s) 219, 818
BseLI CCNNNNNNNGG 4 cut(s) 233, 670, 939, 940
BseNI ACTGG 2 cut(s) 308, 739
BseRI GAGGAG 2 cut(s) 81, 374
BseSI GKGCMC 1 cut(s) 77
BseXI GCAGC 1 cut(s) 700
BseYI CCCAGC 2 cut(s) 307, 599
Bsh1285I CGRYCG 1 cut(s) 36
BshNI GGYRCC 1 cut(s) 74
BsiEI CGRYCG 1 cut(s) 36
BsiHKAI GWGCWC 2 cut(s) 196, 854
BsiSI CCGG 2 cut(s) 39, 227
BslI CCNNNNNNNGG 4 cut(s) 233, 670, 939, 940
BsmAI GTCTC 2 cut(s) 204, 706
BsmI GAATGC 1 cut(s) 298
Bsp1286I GDGCHC 3 cut(s) 77, 196, 854
Bsp143I GATC 1 cut(s) 12
Bsp19I CCATGG 1 cut(s) 398
BspACI CCGC 3 cut(s) 29, 36, 645
BspHI TCATGA 1 cut(s) 288
BspLI GGNNCC 2 cut(s) 76, 175
BspPI GGATC 1 cut(s) 7
BspT107I GGYRCC 1 cut(s) 74
BsrFI RCCGGY 1 cut(s) 226
BsrI ACTGG 2 cut(s) 308, 739
BssAI RCCGGY 1 cut(s) 226
BssECI CCNNGG 2 cut(s) 398, 878
BssMI GATC 1 cut(s) 12
BssNI GRCGYC 1 cut(s) 463
BssT1I CCWWGG 1 cut(s) 398
Bst2UI CCWGG 1 cut(s) 968
Bst4CI ACNGT 3 cut(s) 79, 198, 961
Bst6I CTCTTC 1 cut(s) 501
BstACI GRCGYC 1 cut(s) 463
BstC8I GCNNGC 2 cut(s) 325, 756
BstDSI CCRYGG 1 cut(s) 398
BstF5I GGATG 2 cut(s) 219, 818
BstH2I RGCGCY 1 cut(s) 785
BstHHI GCGC 1 cut(s) 784
BstKTI GATC 1 cut(s) 15
BstMAI GTCTC 2 cut(s) 204, 706
BstMBI GATC 1 cut(s) 12
BstMCI CGRYCG 1 cut(s) 36
BstMWI GCNNNNNNNGC 4 cut(s) 35, 719, 781, 788
BstNI CCWGG 1 cut(s) 968
BstNSI RCATGY 2 cut(s) 711, 896
BstSCI CCNGG 1 cut(s) 966
BstSFI CTRYAG 2 cut(s) 336, 457
BstSLI GKGCMC 1 cut(s) 77
BstV1I GCAGC 1 cut(s) 700
BtgI CCRYGG 1 cut(s) 398
BtsCI GGATG 2 cut(s) 219, 818
BtsIMutI CAGTG 2 cut(s) 194, 957
Cac8I GCNNGC 2 cut(s) 325, 756
CciI TCATGA 1 cut(s) 288
CfoI GCGC 1 cut(s) 784
Cfr10I RCCGGY 1 cut(s) 226
CseI GACGC 1 cut(s) 857
Csp6I GTAC 2 cut(s) 431, 648
CviAII CATG 6 cut(s) 184, 289, 399, 683, 708, 893
CviQI GTAC 2 cut(s) 431, 648
DpnI GATC 1 cut(s) 14
DpnII GATC 1 cut(s) 12
DraI TTTAAA 1 cut(s) 678
Eam1104I CTCTTC 1 cut(s) 501
EarI CTCTTC 1 cut(s) 501
Ecl136II GAGCTC 1 cut(s) 852
Eco130I CCWWGG 1 cut(s) 398
Eco24I GRGCYC 1 cut(s) 854
Eco53kI GAGCTC 1 cut(s) 852
Eco57I CTGAAG 3 cut(s) 47, 299, 762
EcoICRI GAGCTC 1 cut(s) 852
EcoRII CCWGG 1 cut(s) 966
EcoT14I CCWWGG 1 cut(s) 398
EcoT38I GRGCYC 1 cut(s) 854
ErhI CCWWGG 1 cut(s) 398
FaeI CATG 6 cut(s) 187, 292, 402, 686, 711, 896
FalI AAGNNNNNCTT 4 cut(s) 557, 589, 760, 792
FatI CATG 6 cut(s) 183, 288, 398, 682, 707, 892
FauNDI CATATG 2 cut(s) 124, 635
FblI GTMKAC 1 cut(s) 929
Fnu4HI GCNGC 2 cut(s) 30, 714
FokI GGATG 2 cut(s) 206, 825
FriOI GRGCYC 1 cut(s) 854
Fsp4HI GCNGC 2 cut(s) 30, 714
FspBI CTAG 2 cut(s) 608, 954
GlaI GCGC 1 cut(s) 783
GluI GCNGC 2 cut(s) 30, 714
GsaI CCCAGC 2 cut(s) 311, 603
GsuI CTGGAG 1 cut(s) 372
HaeII RGCGCY 1 cut(s) 785
HapII CCGG 2 cut(s) 39, 227
HgaI GACGC 1 cut(s) 857
HhaI GCGC 1 cut(s) 784
Hin1I GRCGYC 1 cut(s) 463
Hin1II CATG 6 cut(s) 187, 292, 402, 686, 711, 896
Hin6I GCGC 1 cut(s) 782
HinP1I GCGC 1 cut(s) 782
HincII GTYRAC 2 cut(s) 301, 467
HindII GTYRAC 2 cut(s) 301, 467
HinfI GANTC 4 cut(s) 8, 453, 527, 944
HpaII CCGG 2 cut(s) 39, 227
HphI GGTGA 5 cut(s) 124, 368, 776, 847, 863
Hpy166II GTNNAC 4 cut(s) 242, 301, 467, 930
Hpy188I TCNGA 4 cut(s) 21, 66, 159, 279
Hpy188III TCNNGA 1 cut(s) 289
Hpy8I GTNNAC 4 cut(s) 242, 301, 467, 930
HpyAV CCTTC 6 cut(s) 26, 64, 274, 786, 884, 1006
HpyCH4III ACNGT 3 cut(s) 79, 198, 961
HpyCH4IV ACGT 3 cut(s) 23, 463, 912
HpyCH4V TGCA 1 cut(s) 982
HpyF10VI GCNNNNNNNGC 4 cut(s) 35, 719, 781, 788
HpySE526I ACGT 3 cut(s) 23, 463, 912
Hsp92I GRCGYC 1 cut(s) 463
Hsp92II CATG 6 cut(s) 187, 292, 402, 686, 711, 896
HspAI GCGC 1 cut(s) 782
Kzo9I GATC 1 cut(s) 12
LmnI GCTCC 4 cut(s) 173, 211, 361, 586
Lsp1109I GCAGC 1 cut(s) 700
LweI GCATC 5 cut(s) 560, 731, 980, 981, 991
MaeI CTAG 2 cut(s) 608, 954
MaeII ACGT 3 cut(s) 23, 463, 912
MaeIII GTNAC 2 cut(s) 730, 869
MalI GATC 1 cut(s) 14
MboI GATC 1 cut(s) 12
MboII GAAGA 6 cut(s) 53, 77, 79, 379, 518, 993
MfeI CAATTG 3 cut(s) 408, 693, 726
MhlI GDGCHC 3 cut(s) 77, 196, 854
MluCI AATT 5 cut(s) 57, 408, 693, 726, 825
MlyI GAGTC 2 cut(s) 17, 953
MmeI TCCRAC 1 cut(s) 972
MnlI CCTC 6 cut(s) 99, 102, 347, 352, 681, 888
MseI TTAA 2 cut(s) 438, 677
MspI CCGG 2 cut(s) 39, 227
MspR9I CCNGG 1 cut(s) 968
MunI CAATTG 3 cut(s) 408, 693, 726
Mva1269I GAATGC 1 cut(s) 298
MvaI CCWGG 1 cut(s) 968
MwoI GCNNNNNNNGC 4 cut(s) 35, 719, 781, 788
NcoI CCATGG 1 cut(s) 398
NdeI CATATG 2 cut(s) 124, 635
NdeII GATC 1 cut(s) 12
NlaIII CATG 6 cut(s) 187, 292, 402, 686, 711, 896
NlaIV GGNNCC 2 cut(s) 76, 175
NmuCI GTSAC 1 cut(s) 869
NspI RCATGY 2 cut(s) 711, 896
PagI TCATGA 1 cut(s) 288
PciI ACATGT 2 cut(s) 707, 892
PcsI WCGNNNNNNNCGW 3 cut(s) 29, 865, 886
PctI GAATGC 1 cut(s) 298
PfeI GAWTC 2 cut(s) 453, 527
PkrI GCNGC 2 cut(s) 31, 715
PleI GAGTC 2 cut(s) 16, 952
PpsI GAGTC 2 cut(s) 16, 952
PscI ACATGT 2 cut(s) 707, 892
PsiI TTATAA 1 cut(s) 824
Psp124BI GAGCTC 1 cut(s) 854
Psp1406I AACGTT 1 cut(s) 912
Psp6I CCWGG 1 cut(s) 966
PspFI CCCAGC 2 cut(s) 307, 599
PspGI CCWGG 1 cut(s) 966
PspN4I GGNNCC 2 cut(s) 76, 175
RsaI GTAC 2 cut(s) 432, 649
RsaNI GTAC 2 cut(s) 431, 648
SacI GAGCTC 1 cut(s) 854
SaqAI TTAA 2 cut(s) 438, 677
SatI GCNGC 2 cut(s) 30, 714
Sau3AI GATC 1 cut(s) 12
SchI GAGTC 2 cut(s) 17, 953
ScrFI CCNGG 1 cut(s) 968
SduI GDGCHC 3 cut(s) 77, 196, 854
SfaNI GCATC 5 cut(s) 560, 731, 980, 981, 991
SfcI CTRYAG 2 cut(s) 336, 457
SmlI CTYRAG 1 cut(s) 107
SmoI CTYRAG 1 cut(s) 107
SpeI ACTAGT 1 cut(s) 607
Sse9I AATT 5 cut(s) 57, 408, 693, 726, 825
SsiI CCGC 3 cut(s) 29, 36, 645
SspMI CTAG 2 cut(s) 608, 954
SstI GAGCTC 1 cut(s) 854
StyD4I CCNGG 1 cut(s) 966
StyI CCWWGG 1 cut(s) 398
TaaI ACNGT 3 cut(s) 79, 198, 961
TaiI ACGT 3 cut(s) 26, 466, 915
TaqI TCGA 2 cut(s) 11, 257
TaqII GACCGA 1 cut(s) 870
TasI AATT 5 cut(s) 57, 408, 693, 726, 825
TatI WGTACW 1 cut(s) 430
TauI GCSGC 1 cut(s) 32
TfiI GAWTC 2 cut(s) 453, 527
Tru1I TTAA 2 cut(s) 438, 677
Tru9I TTAA 2 cut(s) 438, 677
TscAI CASTG 2 cut(s) 201, 964
TseFI GTSAC 1 cut(s) 869
TseI GCWGC 1 cut(s) 713
Tsp45I GTSAC 1 cut(s) 869
TspDTI ATGAA 2 cut(s) 305, 510
TspRI CASTG 2 cut(s) 201, 964
XapI RAATTY 1 cut(s) 57
XceI RCATGY 2 cut(s) 711, 896
XcmI CCANNNNNNNNNTGG 1 cut(s) 230
XmiI GTMKAC 1 cut(s) 929
XspI CTAG 2 cut(s) 608, 954
ZraI GACGTC 1 cut(s) 464
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.