MD15G1114900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0012836
Physical Location & Seq
Forward (+)
8102560 .. 8105893
3334 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1114900.v1.1.491

Sequence Viewer

Length: 954 bp
ATGGTTGGCGGTGGAGGCAGGAGAGACGAGGGCTCGCTCGTCATCAACAACACGAACGTGTTCGCGGCGCTCGAGACTCTGAGGAAGAAGAAGAAGTCCGATAAGGATCGCAAGAGCAAAGGGTCTTCCAAGCCGGCGCAGCCCAAGGCGCCGGAGCCGCAGGTGTTCTGGGCGCCGGCTCCTTTGAACACGAAATCGTGGGCCGATGTTGACGACGAAGATGATGATGATTATTACGCCACTACGGCTCCGCCTCAGGCTGCTTGGGGATCGTCGGAGCCGCAAAAGAGTAAGGAGAAGCCAAATGTAGAGGAAAGTGAGAGTGAAGAAGATATTCTTGATGAAGGTGATGATGATGCAGAGGAAGAGCATGATCATGAACTGGAGGTGCCTGTAAACCCGGAGCCAGTGGTGAAGAAGCCTGCTTATGTTCCTGCGCCACCTAAAGAGGCAGAAAAGCAACTTTCAAAAAAGGAAAGAAAGAAAAAAGAACTTGCAGAACTTGAGGCTCTTCTTGCTGATTTTGGAGTCGCCCCAAAGGAGAGTGATAGTCAAGATGAATCACAAGGTGTTGCACTAGAAAAGGATAATGCACCAAATGGAGATGGAGAAAAGAAGGAAAATCAATCTGCAGAGTCCAAGAGCGCCAAGAAGAAGAAAAAGAAAGATAAAGCTTCCAAGGAGGTGAAAGAATCGCAGGATCAGCCAAACAACTCAGGGGCTACTAATGGTCCGAGTGAAGTTACTGGAGCCGAACAGGCGGAGGAGGATACATCGAATATTGATGTGAAGGAGCGATTAAAGAAGGTTGTATCTGGGAAGAAGAAGAAATCTAGTAAAGAGTTGGATGCTGCAGCAAAAGCTGCTGCTCAAGAGGCTGCTGCTAGGAGAGCACGGCTTGCTGCAGCTAAGAAGAAAGAGAAGAACCATTACAACCAGCAGCCTGTCCGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

318

Amino Acids

34.6

Weight (kDa)

5.44

Isoelectric Point (pI)

49.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012836)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G25670 AT2G25670 AT4G32610
fragaria_vesca FvH4_2g30190
malus_domestica MD15G1114900.v1.1
prunus_persica Prupe.1G466300_v2.0.a1 Prupe.1G466300_v2.0.a1
pyrus_communis pycom15g10350
rosa_chinensis RchiOBHm_Chr6g0312721
rosa_multiflora Rmu_ssc0000412.1_g000011
rosa_roxburghii Rroxscaffold_7G00156560
rosa_rugosa Rorug06G0398600 Rorug06G0398800
rosa_samantha Rh6AG516000 Rh6BG527300 Rh6CG532200 Rh6DG518300
rosa_wichuraiana Rw6G044830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 151
Acc36I ACCTGC 1 cut(s) 151
AccB1I GGYRCC 3 cut(s) 148, 172, 388
AccII CGCG 1 cut(s) 65
AciI CCGC 6 cut(s) 9, 65, 158, 251, 281, 761
AclWI GGATC 3 cut(s) 114, 277, 708
AcyI GRCGYC 2 cut(s) 149, 173
AdeI CACNNNGTG 1 cut(s) 569
AflIII ACRYGT 1 cut(s) 57
AgsI TTSAA 2 cut(s) 187, 468
AleI CACNNNNGTG 1 cut(s) 56
AluBI AGCT 3 cut(s) 674, 863, 908
AluI AGCT 3 cut(s) 674, 863, 908
Alw21I GWGCWC 1 cut(s) 895
Alw26I GTCTC 2 cut(s) 18, 68
AlwI GGATC 3 cut(s) 114, 277, 708
Ama87I CYCGRG 1 cut(s) 71
AoxI GGCC 1 cut(s) 201
Asp700I GAANNNNTTC 2 cut(s) 59, 333
AspLEI GCGC 6 cut(s) 70, 139, 151, 175, 439, 647
AspS9I GGNCC 2 cut(s) 201, 731
AsuC2I CCSGG 1 cut(s) 401
AsuHPI GGTGA 3 cut(s) 359, 424, 697
AvaI CYCGRG 1 cut(s) 71
AvaII GGWCC 1 cut(s) 731
AxyI CCTNAGG 1 cut(s) 255
BanI GGYRCC 3 cut(s) 148, 172, 388
BanII GRGCYC 1 cut(s) 35
BarI GAAGNNNNNNTAC 2 cut(s) 914, 946
BbsI GAAGAC 1 cut(s) 117
Bbv12I GWGCWC 1 cut(s) 895
BccI CCATC 1 cut(s) 599
BceAI ACGGC 2 cut(s) 261, 911
BciVI GTATCC 1 cut(s) 763
BclI TGATCA 1 cut(s) 373
BcnI CCSGG 1 cut(s) 401
BcoDI GTCTC 2 cut(s) 18, 68
BfaI CTAG 3 cut(s) 578, 834, 885
BfmI CTRYAG 3 cut(s) 630, 852, 903
BfoI RGCGCY 4 cut(s) 71, 152, 176, 648
BfuAI ACCTGC 1 cut(s) 151
BfuI GTATCC 1 cut(s) 763
BglI GCCNNNNNGGC 2 cut(s) 245, 758
Bme1390I CCNGG 1 cut(s) 401
Bme18I GGWCC 1 cut(s) 731
BmeT110I CYCGRG 1 cut(s) 71
BmgT120I GGNCC 2 cut(s) 201, 731
BmiI GGNNCC 9 cut(s) 150, 156, 174, 180, 249, 279, 390, 405, 751
BmrFI CCNGG 1 cut(s) 401
BmsI GCATC 2 cut(s) 346, 838
BpiI GAAGAC 1 cut(s) 117
BpmI CTGGAG 2 cut(s) 404, 768
BpuEI CTTGAG 2 cut(s) 524, 855
BpuMI CCSGG 1 cut(s) 401
BsaBI GATNNNNATC 1 cut(s) 105
BsaHI GRCGYC 2 cut(s) 149, 173
BsaJI CCNNGG 2 cut(s) 144, 678
BsaWI WCCGGW 1 cut(s) 948
BsaXI ACNNNNNCTCC 2 cut(s) 232, 262
Bse118I RCCGGY 2 cut(s) 133, 175
Bse1I ACTGG 3 cut(s) 387, 407, 751
Bse21I CCTNAGG 1 cut(s) 255
Bse8I GATNNNNATC 1 cut(s) 105
BseDI CCNNGG 2 cut(s) 144, 678
BseGI GGATG 1 cut(s) 853
BseJI GATNNNNATC 1 cut(s) 105
BseMII CTCAG 3 cut(s) 71, 269, 729
BseNI ACTGG 3 cut(s) 387, 407, 751
BseRI GAGGAG 1 cut(s) 779
Bsh1236I CGCG 1 cut(s) 65
BshFI GGCC 1 cut(s) 203
BshNI GGYRCC 3 cut(s) 148, 172, 388
BsiHKAI GWGCWC 1 cut(s) 895
BsiHKCI CYCGRG 1 cut(s) 71
BsiSI CCGG 5 cut(s) 134, 152, 176, 401, 949
BsmAI GTCTC 2 cut(s) 18, 68
BsmBI CGTCTC 1 cut(s) 18
BsnI GGCC 1 cut(s) 203
BsoBI CYCGRG 1 cut(s) 71
Bsp1286I GDGCHC 2 cut(s) 35, 895
Bsp143I GATC 4 cut(s) 106, 269, 373, 700
BspACI CCGC 6 cut(s) 9, 65, 158, 251, 281, 761
BspANI GGCC 1 cut(s) 203
BspCNI CTCAG 3 cut(s) 72, 268, 728
BspFNI CGCG 1 cut(s) 65
BspHI TCATGA 1 cut(s) 376
BspLI GGNNCC 9 cut(s) 150, 156, 174, 180, 249, 279, 390, 405, 751
BspMAI CTGCAG 3 cut(s) 634, 856, 907
BspMI ACCTGC 1 cut(s) 151
BspPI GGATC 3 cut(s) 114, 277, 708
BspQI GCTCTTC 2 cut(s) 360, 516
BspT107I GGYRCC 3 cut(s) 148, 172, 388
BsrFI RCCGGY 2 cut(s) 133, 175
BsrI ACTGG 3 cut(s) 387, 407, 751
BssAI RCCGGY 2 cut(s) 133, 175
BssECI CCNNGG 2 cut(s) 144, 678
BssMI GATC 4 cut(s) 106, 269, 373, 700
BssNI GRCGYC 2 cut(s) 149, 173
BssT1I CCWWGG 2 cut(s) 144, 678
Bst6I CTCTTC 2 cut(s) 360, 516
BstACI GRCGYC 2 cut(s) 149, 173
BstAPI GCANNNNNTGC 2 cut(s) 863, 899
BstC8I GCNNGC 5 cut(s) 35, 135, 177, 423, 900
BstDEI CTNAG 4 cut(s) 80, 255, 715, 909
BstF5I GGATG 1 cut(s) 853
BstFNI CGCG 1 cut(s) 65
BstH2I RGCGCY 4 cut(s) 71, 152, 176, 648
BstHHI GCGC 6 cut(s) 70, 139, 151, 175, 439, 647
BstKTI GATC 4 cut(s) 109, 272, 376, 703
BstMAI GTCTC 2 cut(s) 18, 68
BstMBI GATC 4 cut(s) 106, 269, 373, 700
BstSCI CCNGG 1 cut(s) 399
BstSFI CTRYAG 3 cut(s) 630, 852, 903
BstUI CGCG 1 cut(s) 65
BstV2I GAAGAC 1 cut(s) 117
Bsu36I CCTNAGG 1 cut(s) 255
BsuI GTATCC 1 cut(s) 763
BsuRI GGCC 1 cut(s) 203
BtsCI GGATG 1 cut(s) 853
BtsIMutI CAGTG 1 cut(s) 414
BveI ACCTGC 1 cut(s) 151
Cac8I GCNNGC 5 cut(s) 35, 135, 177, 423, 900
CciI TCATGA 1 cut(s) 376
CfoI GCGC 6 cut(s) 70, 139, 151, 175, 439, 647
Cfr10I RCCGGY 2 cut(s) 133, 175
Cfr13I GGNCC 2 cut(s) 201, 731
CviAII CATG 2 cut(s) 371, 377
DdeI CTNAG 4 cut(s) 80, 255, 715, 909
DinI GGCGCC 2 cut(s) 150, 174
DpnI GATC 4 cut(s) 108, 271, 375, 702
DpnII GATC 4 cut(s) 106, 269, 373, 700
DraIII CACNNNGTG 1 cut(s) 569
Eam1104I CTCTTC 2 cut(s) 360, 516
EarI CTCTTC 2 cut(s) 360, 516
EciI GGCGGA 2 cut(s) 240, 776
Eco130I CCWWGG 2 cut(s) 144, 678
Eco24I GRGCYC 1 cut(s) 35
Eco47I GGWCC 1 cut(s) 731
Eco81I CCTNAGG 1 cut(s) 255
Eco88I CYCGRG 1 cut(s) 71
EcoT14I CCWWGG 2 cut(s) 144, 678
EcoT38I GRGCYC 1 cut(s) 35
EgeI GGCGCC 2 cut(s) 150, 174
EheI GGCGCC 2 cut(s) 150, 174
ErhI CCWWGG 2 cut(s) 144, 678
Esp3I CGTCTC 1 cut(s) 18
FaeI CATG 2 cut(s) 374, 380
FaiI YATR 3 cut(s) 372, 378, 429
FalI AAGNNNNNCTT 2 cut(s) 321, 353
FatI CATG 2 cut(s) 370, 376
FbaI TGATCA 1 cut(s) 373
FokI GGATG 1 cut(s) 860
FriOI GRGCYC 1 cut(s) 35
FspBI CTAG 3 cut(s) 578, 834, 885
GlaI GCGC 6 cut(s) 69, 138, 150, 174, 438, 646
GsuI CTGGAG 2 cut(s) 404, 768
HaeII RGCGCY 4 cut(s) 71, 152, 176, 648
HaeIII GGCC 1 cut(s) 203
HapII CCGG 5 cut(s) 134, 152, 176, 401, 949
HhaI GCGC 6 cut(s) 70, 139, 151, 175, 439, 647
Hin1I GRCGYC 2 cut(s) 149, 173
Hin1II CATG 2 cut(s) 374, 380
Hin6I GCGC 6 cut(s) 68, 137, 149, 173, 437, 645
HinP1I GCGC 6 cut(s) 68, 137, 149, 173, 437, 645
HincII GTYRAC 1 cut(s) 211
HindII GTYRAC 1 cut(s) 211
HindIII AAGCTT 1 cut(s) 672
HinfI GANTC 5 cut(s) 76, 528, 560, 635, 692
HpaII CCGG 5 cut(s) 134, 152, 176, 401, 949
HphI GGTGA 3 cut(s) 359, 424, 697
Hpy166II GTNNAC 2 cut(s) 211, 397
Hpy188I TCNGA 4 cut(s) 81, 100, 277, 735
Hpy188III TCNNGA 5 cut(s) 73, 338, 377, 554, 872
Hpy8I GTNNAC 2 cut(s) 211, 397
Hpy99I CGWCG 2 cut(s) 218, 277
HpyAV CCTTC 4 cut(s) 338, 610, 784, 799
HpyCH4IV ACGT 1 cut(s) 57
HpyCH4V TGCA 7 cut(s) 359, 497, 575, 593, 632, 854, 905
HpyF3I CTNAG 4 cut(s) 80, 255, 715, 909
HpySE526I ACGT 1 cut(s) 57
Hsp92I GRCGYC 2 cut(s) 149, 173
Hsp92II CATG 2 cut(s) 374, 380
HspAI GCGC 6 cut(s) 68, 137, 149, 173, 437, 645
KasI GGCGCC 2 cut(s) 148, 172
KroI GCCGGC 2 cut(s) 133, 175
KroNI GCCGGC 2 cut(s) 135, 177
Ksp22I TGATCA 1 cut(s) 373
Kzo9I GATC 4 cut(s) 106, 269, 373, 700
LguI GCTCTTC 2 cut(s) 360, 516
LmnI GCTCC 7 cut(s) 154, 184, 253, 277, 403, 749, 793
LweI GCATC 2 cut(s) 346, 838
MaeI CTAG 3 cut(s) 578, 834, 885
MaeII ACGT 1 cut(s) 57
MaeIII GTNAC 1 cut(s) 742
MalI GATC 4 cut(s) 108, 271, 375, 702
MboI GATC 4 cut(s) 106, 269, 373, 700
MhlI GDGCHC 2 cut(s) 35, 895
Mly113I GGCGCC 2 cut(s) 149, 173
MlyI GAGTC 3 cut(s) 70, 537, 644
MmeI TCCRAC 2 cut(s) 255, 825
MroNI GCCGGC 2 cut(s) 133, 175
MroXI GAANNNNTTC 2 cut(s) 59, 333
MseI TTAA 1 cut(s) 800
MslI CAYNNNNRTG 2 cut(s) 56, 375
MspI CCGG 5 cut(s) 134, 152, 176, 401, 949
MspR9I CCNGG 1 cut(s) 401
MvnI CGCG 1 cut(s) 65
NaeI GCCGGC 2 cut(s) 135, 177
NarI GGCGCC 2 cut(s) 149, 173
NciI CCSGG 1 cut(s) 401
NdeII GATC 4 cut(s) 106, 269, 373, 700
NgoMIV GCCGGC 2 cut(s) 133, 175
NlaIII CATG 2 cut(s) 374, 380
NlaIV GGNNCC 9 cut(s) 150, 156, 174, 180, 249, 279, 390, 405, 751
OliI CACNNNNGTG 1 cut(s) 56
PaeR7I CTCGAG 1 cut(s) 71
PagI TCATGA 1 cut(s) 376
PaqCI CACCTGC 1 cut(s) 151
PciSI GCTCTTC 2 cut(s) 360, 516
PcsI WCGNNNNNNNCGW 1 cut(s) 69
PdiI GCCGGC 2 cut(s) 135, 177
PdmI GAANNNNTTC 2 cut(s) 59, 333
PfeI GAWTC 2 cut(s) 560, 692
PleI GAGTC 3 cut(s) 70, 536, 643
PluTI GGCGCC 2 cut(s) 152, 176
PpsI GAGTC 3 cut(s) 70, 536, 643
PspN4I GGNNCC 9 cut(s) 150, 156, 174, 180, 249, 279, 390, 405, 751
PspPI GGNCC 2 cut(s) 201, 731
PstI CTGCAG 3 cut(s) 634, 856, 907
RseI CAYNNNNRTG 2 cut(s) 56, 375
SapI GCTCTTC 2 cut(s) 360, 516
SaqAI TTAA 1 cut(s) 800
Sau3AI GATC 4 cut(s) 106, 269, 373, 700
Sau96I GGNCC 2 cut(s) 201, 731
SchI GAGTC 3 cut(s) 70, 537, 644
ScrFI CCNGG 1 cut(s) 401
SduI GDGCHC 2 cut(s) 35, 895
SfaNI GCATC 2 cut(s) 346, 838
SfcI CTRYAG 3 cut(s) 630, 852, 903
SfoI GGCGCC 2 cut(s) 150, 174
Sfr274I CTCGAG 1 cut(s) 71
SinI GGWCC 1 cut(s) 731
SlaI CTCGAG 1 cut(s) 71
SmiMI CAYNNNNRTG 2 cut(s) 56, 375
SmlI CTYRAG 3 cut(s) 71, 503, 870
SmoI CTYRAG 3 cut(s) 71, 503, 870
SsiI CCGC 6 cut(s) 9, 65, 158, 251, 281, 761
SspDI GGCGCC 2 cut(s) 148, 172
SspI AATATT 1 cut(s) 781
SspMI CTAG 3 cut(s) 578, 834, 885
StyD4I CCNGG 1 cut(s) 399
StyI CCWWGG 2 cut(s) 144, 678
TaiI ACGT 1 cut(s) 60
TaqI TCGA 2 cut(s) 72, 776
TauI GCSGC 3 cut(s) 68, 160, 283
TfiI GAWTC 2 cut(s) 560, 692
Tru1I TTAA 1 cut(s) 800
Tru9I TTAA 1 cut(s) 800
TscAI CASTG 1 cut(s) 414
TspDTI ATGAA 3 cut(s) 357, 393, 573
TspRI CASTG 1 cut(s) 414
VpaK11BI GGWCC 1 cut(s) 731
XhoI CTCGAG 1 cut(s) 71
XmnI GAANNNNTTC 2 cut(s) 59, 333
XspI CTAG 3 cut(s) 578, 834, 885
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.