Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0012330

Physical Location & Seq

Genomic Coordinates Reverse (-)

16892883 .. 16898999

Gene Structure 6117 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1210700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1587 bp

ATGGCGATGGCGGATTCGCCTGAGGCAGTAGTCATCGCAGGCGCCACCACAAGCCCCCGCGCGGACTCAGATCTCAACCACACGCTACGCCGCAGACCCTCGGCCTCCACCGTGGCGGAATCCGATTCCTTGAACGATTTGGTTAGGGACTCCGGCGGTGATGACTCGGTTGGCGCCAAGCTCGGCGGCGGCGGCGGCGGCGATAACGGCCGTATTGGAGGCTCTGACGGCTCGGCTGAGTCCGTCAATGCTGTTCAAGACAAGAAGGGACTCGGCGAGAATGATCGGGGCGGTGATCCCTCGGCCGTGAAATTTGCGTATCGGCCGTCCGTTCCGGCTCATCGGAGAGTTAAGGAGAGTCCTCTCAGCTCTGATGCCATCTTCAGACAGAGCCATGCCGGTCTCATCAATCTGTGTATAGTAGTACTTGTTGCTGTAAACAGCCGGCTAATCATCGAGAATTTAATGAAGTATGGTTGGTTAATTAAAACTGGTTTCTGGTTTAGTTCAAGATCATTGCGAGATTGGCCACTTTTTATGTGCGGCCTTACTCTTCCTATCTTTCCACTTGCGGCCTTTGTAGTTGAGAAGTTAGCGCAACAAAAGTGTATATCTGAAGCTGCTGTTGTTTCTCTTCATATAGTCATTACAACAACTTCAATTTTGTATCCGGTTGTGGTGATTCTCAGGTGTGATTCTGCAGTTTTATTTGGTGTTGCGTTGATGCTCTTTGCTTGCATCGTATGGTTAAAACTCGTATCTTATGTGCACACAAACTATGATATGAGAGCACTTCACATAGCAGTTGAGAAGGGGGATGCCACACCTAGTTCTCTGAATACAGATTACCCTCATAATAACAATTTAAAAAATATGGCATACTTTATGGTCGCTCCAACATTATGTTACCAGACTAGTTACCCTCGCACAGCATGTGTTCGAAAAGGTTGGGTGTTTCGTCAACTTGTGAAGTTGATCATTTTTACTGGAGTGATGGGATTTATAGTAGAACAATACATCAACCCTATTGTCAAGAATTCAAAACATCCTTTGAAGGGGGACTGGTTATATGCCATAGAGAGGGTTTTGAAGCTTTCAGTTCCCAATTTATATGTGTGGCTATGCATGTTCTATTGCTTTTTCCACCTCTGGTTAAATATTGTTGCTGAGCTGCTTTGTTTTGGTGATCGGGAGTTCTATAAAGATTGGTGGAATGCAAAAACTGTTGAGGAGTATTGGAGAATGTGGAACATGCCTGTTCATAAATGGATGGTTCGCCACATCTATTTTCCATGCCTACGTCACGGAGTACCCAAGGGAGTTGCCATCGTAATCGCCTTCCTAGTATCTGCCGTATTTCATGAGCTATGCATAGCTGTTCCCTGCCACATTTTCAAGTTGTGGGCTTTTATCGGAATTATGTTCCAGGTACCACTTGTCTTGATCACAAATTACCTGCAAAATAAATTCAGAAACTCGATGGTGGGAAATATGATCTTCTGGTTCATCTTTTGCATTCTTGGTCAACCGATGTGCCTGCTTCTGTATTACCACGACTTGATGAATCGGAAAGGGAAACTCGACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001101 GO:0003006 GO:0003674 GO:0003824 GO:0004144 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0005811 GO:0005975 GO:0006629 GO:0006638 GO:0006639 GO:0006641 GO:0006950 GO:0006970 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0008374 GO:0008610 GO:0009058 GO:0009266 GO:0009409 GO:0009507 GO:0009526 GO:0009536 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0009743 GO:0009746 GO:0009749 GO:0009790 GO:0009791 GO:0009793 GO:0009941 GO:0009987 GO:0010029 GO:0010030 GO:0010033 GO:0010154 GO:0012505 GO:0016020 GO:0016411 GO:0016740 GO:0016746 GO:0016747 GO:0019432 GO:0022414 GO:0031967 GO:0031975 GO:0031984 GO:0032501 GO:0032502 GO:0033993 GO:0034284 GO:0042175 GO:0042221 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045017 GO:0045995 GO:0046460 GO:0046463 GO:0046486 GO:0048316 GO:0048518 GO:0048580 GO:0048582 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050896 GO:0051094 GO:0051239 GO:0051240 GO:0061458 GO:0065007 GO:0071704 GO:0097305 GO:0098827 GO:1900140 GO:1901576 GO:1901700 GO:2000026

KEGG Pathways

Metabolic & Signaling

ko00561 ko00830 ko01100 ko04975 map00561 map00830 map01100 map04975

Pfam Domains

Protein Families

MBOAT

Protein Analysis

529

Amino Acids

59.47

Weight (kDa)

8.97

Isoelectric Point (pI)

46.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MBOAT	PF03062	270 - 515	6.1e-27	MBOAT, membrane-bound O-acyltransferase family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012330)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G19450
fragaria_vesca	FvH4_1g07910
malus_domestica	MD02G1082600.v1.1 MD15G1210700.v1.1
prunus_persica	Prupe.7G206100_v2.0.a1
pyrus_communis	pycom02g06550 pycom15g18660
rosa_chinensis	RchiOBHm_Chr2g0093781
rosa_laevigata	RLG00000016436
rosa_multiflora	Rmu_sc0002126.1_g000005 Rmu_sc0012116.1_g000002
rosa_roxburghii	Rroxscaffold_2G00147810
rosa_rugosa	Rorug02G0039700 Rorug02G0039800 Rorug02G0039900 Rorug02G0040000
rosa_samantha	Rh2AG086200 Rh2BG086500 Rh2DG084600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1464
Acc65I	GGTACC	1 cut(s)	1429
AccB1I	GGYRCC	3 cut(s)	41, 173, 1429
AccII	CGCG	2 cut(s)	60, 62
AclWI	GGATC	1 cut(s)	290
AcoI	YGGCCR	4 cut(s)	208, 303, 323, 527
AcsI	RAATTY	4 cut(s)	311, 460, 1036, 1466
AcuI	CTGAAG	2 cut(s)	367, 636
AcyI	GRCGYC	2 cut(s)	42, 174
AfaI	GTAC	3 cut(s)	426, 1311, 1431
AfiI	CCNNNNNNNGG	3 cut(s)	61, 1055, 1080
AgsI	TTSAA	8 cut(s)	133, 257, 510, 660, 1041, 1054, 1090, 1396
AhlI	ACTAGT	1 cut(s)	914
AjnI	CCWGG	1 cut(s)	1425
AluBI	AGCT	7 cut(s)	181, 369, 620, 1093, 1171, 1366, 1376
AluI	AGCT	7 cut(s)	181, 369, 620, 1093, 1171, 1366, 1376
Alw21I	GWGCWC	2 cut(s)	771, 793
Alw26I	GTCTC	1 cut(s)	407
Alw44I	GTGCAC	1 cut(s)	767
AlwI	GGATC	1 cut(s)	290
AoxI	GGCC	7 cut(s)	102, 208, 303, 323, 527, 544, 573
ApaLI	GTGCAC	1 cut(s)	767
ApeKI	GCWGC	2 cut(s)	620, 1171
ApoI	RAATTY	4 cut(s)	311, 460, 1036, 1466
Asp718I	GGTACC	1 cut(s)	1429
AspLEI	GCGC	4 cut(s)	44, 62, 176, 598
AsuHPI	GGTGA	4 cut(s)	170, 305, 691, 1196
AsuII	TTCGAA	1 cut(s)	940
AxyI	CCTNAGG	1 cut(s)	21
BaeGI	GKGCMC	1 cut(s)	771
BalI	TGGCCA	1 cut(s)	529
BanI	GGYRCC	3 cut(s)	41, 173, 1429
Bbv12I	GWGCWC	2 cut(s)	771, 793
BbvI	GCAGC	2 cut(s)	607, 1158
BccI	CCATC	5 cut(s)	386, 988, 1264, 1334, 1474
BceAI	ACGGC	6 cut(s)	195, 223, 244, 290, 310, 1337
BcgI	CGANNNNNNTGC	2 cut(s)	1519, 1553
BciT130I	CCWGG	1 cut(s)	1427
BciVI	GTATCC	1 cut(s)	678
BclI	TGATCA	2 cut(s)	975, 1443
BcoDI	GTCTC	1 cut(s)	407
BcuI	ACTAGT	1 cut(s)	914
BfaI	CTAG	3 cut(s)	828, 915, 1343
BfmI	CTRYAG	1 cut(s)	699
BfoI	RGCGCY	2 cut(s)	45, 177
BfuAI	ACCTGC	1 cut(s)	1464
BfuI	GTATCC	1 cut(s)	678
BglII	AGATCT	1 cut(s)	70
BlpI	GCTNAGC	1 cut(s)	1167
BmcAI	AGTACT	1 cut(s)	426
Bme1390I	CCNGG	1 cut(s)	1427
BmiI	GGNNCC	3 cut(s)	43, 175, 1431
BmrFI	CCNGG	1 cut(s)	1427
BmsI	GCATC	4 cut(s)	364, 714, 747, 808
BpmI	CTGGAG	1 cut(s)	1008
Bpu1102I	GCTNAGC	1 cut(s)	1167
Bpu14I	TTCGAA	1 cut(s)	940
BsaHI	GRCGYC	2 cut(s)	42, 174
BsaI	GGTCTC	1 cut(s)	407
BsaJI	CCNNGG	4 cut(s)	99, 111, 300, 1314
BsaWI	WCCGGW	1 cut(s)	670
Bsc4I	CCNNNNNNNGG	3 cut(s)	61, 1055, 1080
Bse118I	RCCGGY	2 cut(s)	398, 444
Bse1I	ACTGG	3 cut(s)	496, 991, 1067
Bse21I	CCTNAGG	1 cut(s)	21
Bse3DI	GCAATG	1 cut(s)	515
BseBI	CCWGG	1 cut(s)	1427
BseDI	CCNNGG	4 cut(s)	99, 111, 300, 1314
BseGI	GGATG	3 cut(s)	823, 1045, 1275
BseLI	CCNNNNNNNGG	3 cut(s)	61, 1055, 1080
BseMI	GCAATG	1 cut(s)	515
BseMII	CTCAG	6 cut(s)	12, 81, 228, 379, 700, 1158
BseNI	ACTGG	3 cut(s)	496, 991, 1067
BseRI	GAGGAG	1 cut(s)	1244
BseSI	GKGCMC	1 cut(s)	771
BseX3I	CGGCCG	3 cut(s)	208, 303, 323
BseXI	GCAGC	2 cut(s)	607, 1158
Bsh1236I	CGCG	2 cut(s)	60, 62
Bsh1285I	CGRYCG	3 cut(s)	211, 306, 326
BshFI	GGCC	7 cut(s)	104, 210, 305, 325, 529, 546, 575
BshNI	GGYRCC	3 cut(s)	41, 173, 1429
BsiEI	CGRYCG	3 cut(s)	211, 306, 326
BsiHKAI	GWGCWC	2 cut(s)	771, 793
BsiSI	CCGG	5 cut(s)	153, 335, 399, 445, 671
BslFI	GGGAC	3 cut(s)	161, 282, 1073
BslI	CCNNNNNNNGG	3 cut(s)	61, 1055, 1080
BsmAI	GTCTC	1 cut(s)	407
BsmFI	GGGAC	3 cut(s)	161, 282, 1073
BsmI	GAATGC	2 cut(s)	1219, 1515
BsnI	GGCC	7 cut(s)	104, 210, 305, 325, 529, 546, 575
Bso31I	GGTCTC	1 cut(s)	407
Bsp119I	TTCGAA	1 cut(s)	940
Bsp1286I	GDGCHC	2 cut(s)	771, 793
Bsp143I	GATC	8 cut(s)	70, 283, 295, 512, 975, 1186, 1443, 1494
Bsp1720I	GCTNAGC	1 cut(s)	1167
BspANI	GGCC	7 cut(s)	104, 210, 305, 325, 529, 546, 575
BspCNI	CTCAG	6 cut(s)	13, 80, 229, 378, 699, 1159
BspFNI	CGCG	2 cut(s)	60, 62
BspHI	TCATGA	1 cut(s)	1360
BspLI	GGNNCC	3 cut(s)	43, 175, 1431
BspMAI	CTGCAG	1 cut(s)	703
BspMI	ACCTGC	1 cut(s)	1464
BspPI	GGATC	1 cut(s)	290
BspT104I	TTCGAA	1 cut(s)	940
BspT107I	GGYRCC	3 cut(s)	41, 173, 1429
BspTNI	GGTCTC	1 cut(s)	407
BsrDI	GCAATG	1 cut(s)	515
BsrFI	RCCGGY	2 cut(s)	398, 444
BsrI	ACTGG	3 cut(s)	496, 991, 1067
BssAI	RCCGGY	2 cut(s)	398, 444
BssECI	CCNNGG	4 cut(s)	99, 111, 300, 1314
BssMI	GATC	8 cut(s)	70, 283, 295, 512, 975, 1186, 1443, 1494
BssNI	GRCGYC	2 cut(s)	42, 174
BssT1I	CCWWGG	1 cut(s)	1314
Bst2UI	CCWGG	1 cut(s)	1427
Bst4CI	ACNGT	2 cut(s)	112, 1225
Bst6I	CTCTTC	2 cut(s)	558, 639
BstACI	GRCGYC	2 cut(s)	42, 174
BstBI	TTCGAA	1 cut(s)	940
BstC8I	GCNNGC	4 cut(s)	40, 446, 736, 1538
BstDEI	CTNAG	6 cut(s)	21, 67, 237, 365, 686, 1167
BstDSI	CCRYGG	1 cut(s)	111
BstF5I	GGATG	3 cut(s)	823, 1045, 1275
BstFNI	CGCG	2 cut(s)	60, 62
BstH2I	RGCGCY	2 cut(s)	45, 177
BstHHI	GCGC	4 cut(s)	44, 62, 176, 598
BstKTI	GATC	8 cut(s)	73, 286, 298, 515, 978, 1189, 1446, 1497
BstMAI	GTCTC	1 cut(s)	407
BstMBI	GATC	8 cut(s)	70, 283, 295, 512, 975, 1186, 1443, 1494
BstMCI	CGRYCG	3 cut(s)	211, 306, 326
BstMWI	GCNNNNNNNGC	6 cut(s)	192, 195, 198, 207, 228, 526
BstNI	CCWGG	1 cut(s)	1427
BstNSI	RCATGY	3 cut(s)	936, 1129, 1255
BstSCI	CCNGG	1 cut(s)	1425
BstSFI	CTRYAG	1 cut(s)	699
BstSLI	GKGCMC	1 cut(s)	771
BstUI	CGCG	2 cut(s)	60, 62
BstV1I	GCAGC	2 cut(s)	607, 1158
BstX2I	RGATCY	1 cut(s)	70
BstYI	RGATCY	1 cut(s)	70
BstZI	CGGCCG	3 cut(s)	208, 303, 323
Bsu36I	CCTNAGG	1 cut(s)	21
BsuI	GTATCC	1 cut(s)	678
BsuRI	GGCC	7 cut(s)	104, 210, 305, 325, 529, 546, 575
BtgI	CCRYGG	1 cut(s)	111
BtgZI	GCGATG	2 cut(s)	19, 20
BtsCI	GGATG	3 cut(s)	823, 1045, 1275
BveI	ACCTGC	1 cut(s)	1464
Cac8I	GCNNGC	4 cut(s)	40, 446, 736, 1538
CciI	TCATGA	1 cut(s)	1360
CfoI	GCGC	4 cut(s)	44, 62, 176, 598
Cfr10I	RCCGGY	2 cut(s)	398, 444
Csp6I	GTAC	3 cut(s)	425, 1310, 1430
CspCI	CAANNNNNGTGG	2 cut(s)	1422, 1457
CviAII	CATG	6 cut(s)	395, 933, 1126, 1252, 1293, 1361
CviQI	GTAC	3 cut(s)	425, 1310, 1430
DdeI	CTNAG	6 cut(s)	21, 67, 237, 365, 686, 1167
DinI	GGCGCC	2 cut(s)	43, 175
DpnI	GATC	8 cut(s)	72, 285, 297, 514, 977, 1188, 1445, 1496
DpnII	GATC	8 cut(s)	70, 283, 295, 512, 975, 1186, 1443, 1494
DraI	TTTAAA	1 cut(s)	867
EaeI	YGGCCR	4 cut(s)	208, 303, 323, 527
EagI	CGGCCG	3 cut(s)	208, 303, 323
Eam1104I	CTCTTC	2 cut(s)	558, 639
EarI	CTCTTC	2 cut(s)	558, 639
EciI	GGCGGA	2 cut(s)	26, 131
EclXI	CGGCCG	3 cut(s)	208, 303, 323
Eco130I	CCWWGG	1 cut(s)	1314
Eco31I	GGTCTC	1 cut(s)	407
Eco52I	CGGCCG	3 cut(s)	208, 303, 323
Eco57I	CTGAAG	2 cut(s)	367, 636
Eco81I	CCTNAGG	1 cut(s)	21
EcoRI	GAATTC	1 cut(s)	1036
EcoRII	CCWGG	1 cut(s)	1425
EcoT14I	CCWWGG	1 cut(s)	1314
EcoT22I	ATGCAT	2 cut(s)	1127, 1373
EgeI	GGCGCC	2 cut(s)	43, 175
EheI	GGCGCC	2 cut(s)	43, 175
ErhI	CCWWGG	1 cut(s)	1314
FaeI	CATG	6 cut(s)	398, 936, 1129, 1255, 1296, 1364
FaqI	GGGAC	3 cut(s)	161, 282, 1073
FatI	CATG	6 cut(s)	394, 932, 1125, 1251, 1292, 1360
FauI	CCCGC	1 cut(s)	65
FbaI	TGATCA	2 cut(s)	975, 1443
FokI	GGATG	3 cut(s)	830, 1032, 1282
FspBI	CTAG	3 cut(s)	828, 915, 1343
GlaI	GCGC	4 cut(s)	43, 61, 175, 597
GsuI	CTGGAG	1 cut(s)	1008
HaeII	RGCGCY	2 cut(s)	45, 177
HaeIII	GGCC	7 cut(s)	104, 210, 305, 325, 529, 546, 575
HapII	CCGG	5 cut(s)	153, 335, 399, 445, 671
HhaI	GCGC	4 cut(s)	44, 62, 176, 598
Hin1I	GRCGYC	2 cut(s)	42, 174
Hin1II	CATG	6 cut(s)	398, 936, 1129, 1255, 1296, 1364
Hin6I	GCGC	4 cut(s)	42, 60, 174, 596
HinP1I	GCGC	4 cut(s)	42, 60, 174, 596
HincII	GTYRAC	2 cut(s)	962, 1526
HindII	GTYRAC	2 cut(s)	962, 1526
HindIII	AAGCTT	1 cut(s)	1091
HpaII	CCGG	5 cut(s)	153, 335, 399, 445, 671
HphI	GGTGA	4 cut(s)	170, 305, 691, 1196
Hpy166II	GTNNAC	4 cut(s)	439, 769, 962, 1526
Hpy188III	TCNNGA	7 cut(s)	257, 457, 510, 1033, 1190, 1361, 1441
Hpy8I	GTNNAC	4 cut(s)	439, 769, 962, 1526
HpyAV	CCTTC	4 cut(s)	259, 805, 1048, 1348
HpyCH4III	ACNGT	2 cut(s)	112, 1225
HpyCH4IV	ACGT	1 cut(s)	1300
HpyCH4V	TGCA	8 cut(s)	701, 738, 769, 1125, 1217, 1371, 1459, 1515
HpyF10VI	GCNNNNNNNGC	6 cut(s)	192, 195, 198, 207, 228, 526
HpyF3I	CTNAG	6 cut(s)	21, 67, 237, 365, 686, 1167
HpySE526I	ACGT	1 cut(s)	1300
Hsp92I	GRCGYC	2 cut(s)	42, 174
Hsp92II	CATG	6 cut(s)	398, 936, 1129, 1255, 1296, 1364
HspAI	GCGC	4 cut(s)	42, 60, 174, 596
KasI	GGCGCC	2 cut(s)	41, 173
KpnI	GGTACC	1 cut(s)	1433
KroI	GCCGGC	1 cut(s)	444
KroNI	GCCGGC	1 cut(s)	446
Ksp22I	TGATCA	2 cut(s)	975, 1443
Kzo9I	GATC	8 cut(s)	70, 283, 295, 512, 975, 1186, 1443, 1494
LmnI	GCTCC	1 cut(s)	898
Lsp1109I	GCAGC	2 cut(s)	607, 1158
LweI	GCATC	4 cut(s)	364, 714, 747, 808
MaeI	CTAG	3 cut(s)	828, 915, 1343
MaeII	ACGT	1 cut(s)	1300
MaeIII	GTNAC	3 cut(s)	905, 917, 1301
MalI	GATC	8 cut(s)	72, 285, 297, 514, 977, 1188, 1445, 1496
MboI	GATC	8 cut(s)	70, 283, 295, 512, 975, 1186, 1443, 1494
MboII	GAAGA	4 cut(s)	373, 545, 626, 1489
MflI	RGATCY	1 cut(s)	70
MhlI	GDGCHC	2 cut(s)	771, 793
MlsI	TGGCCA	1 cut(s)	529
MluNI	TGGCCA	1 cut(s)	529
Mly113I	GGCGCC	2 cut(s)	42, 174
MlyI	GAGTC	6 cut(s)	59, 143, 158, 248, 264, 367
MmeI	TCCRAC	1 cut(s)	920
Mox20I	TGGCCA	1 cut(s)	529
Mph1103I	ATGCAT	2 cut(s)	1127, 1373
MroNI	GCCGGC	1 cut(s)	444
MscI	TGGCCA	1 cut(s)	529
MseI	TTAA	7 cut(s)	351, 464, 482, 486, 749, 866, 1154
Msp20I	TGGCCA	1 cut(s)	529
MspI	CCGG	5 cut(s)	153, 335, 399, 445, 671
MspR9I	CCNGG	1 cut(s)	1427
Mva1269I	GAATGC	2 cut(s)	1219, 1515
MvaI	CCWGG	1 cut(s)	1427
MvnI	CGCG	2 cut(s)	60, 62
MwoI	GCNNNNNNNGC	6 cut(s)	192, 195, 198, 207, 228, 526
NaeI	GCCGGC	1 cut(s)	446
NarI	GGCGCC	2 cut(s)	42, 174
NdeII	GATC	8 cut(s)	70, 283, 295, 512, 975, 1186, 1443, 1494
NgoMIV	GCCGGC	1 cut(s)	444
NlaIII	CATG	6 cut(s)	398, 936, 1129, 1255, 1296, 1364
NlaIV	GGNNCC	3 cut(s)	43, 175, 1431
NmeAIII	GCCGAG	5 cut(s)	80, 162, 212, 252, 281
NmuCI	GTSAC	1 cut(s)	1301
NsiI	ATGCAT	2 cut(s)	1127, 1373
NspI	RCATGY	3 cut(s)	936, 1129, 1255
NspV	TTCGAA	1 cut(s)	940
PacI	TTAATTAA	1 cut(s)	486
PagI	TCATGA	1 cut(s)	1360
PctI	GAATGC	2 cut(s)	1219, 1515
PdiI	GCCGGC	1 cut(s)	446
PfeI	GAWTC	6 cut(s)	14, 119, 125, 682, 695, 1564
PleI	GAGTC	6 cut(s)	59, 143, 158, 247, 264, 366
PluTI	GGCGCC	2 cut(s)	45, 177
PpsI	GAGTC	6 cut(s)	59, 143, 158, 247, 264, 366
Psp6I	CCWGG	1 cut(s)	1425
PspGI	CCWGG	1 cut(s)	1425
PspN4I	GGNNCC	3 cut(s)	43, 175, 1431
PstI	CTGCAG	1 cut(s)	703
PsuI	RGATCY	1 cut(s)	70
RsaI	GTAC	3 cut(s)	426, 1311, 1431
RsaNI	GTAC	3 cut(s)	425, 1310, 1430
SaqAI	TTAA	7 cut(s)	351, 464, 482, 486, 749, 866, 1154
Sau3AI	GATC	8 cut(s)	70, 283, 295, 512, 975, 1186, 1443, 1494
ScaI	AGTACT	1 cut(s)	426
SchI	GAGTC	6 cut(s)	59, 143, 158, 248, 264, 367
ScrFI	CCNGG	1 cut(s)	1427
SduI	GDGCHC	2 cut(s)	771, 793
SfaNI	GCATC	4 cut(s)	364, 714, 747, 808
SfcI	CTRYAG	1 cut(s)	699
SfoI	GGCGCC	2 cut(s)	43, 175
SfuI	TTCGAA	1 cut(s)	940
SpeI	ACTAGT	1 cut(s)	914
SspDI	GGCGCC	2 cut(s)	41, 173
SspI	AATATT	1 cut(s)	1159
SspMI	CTAG	3 cut(s)	828, 915, 1343
StyD4I	CCNGG	1 cut(s)	1425
StyI	CCWWGG	1 cut(s)	1314
TaaI	ACNGT	2 cut(s)	112, 1225
TaiI	ACGT	1 cut(s)	1303
TaqI	TCGA	4 cut(s)	456, 940, 1478, 1581
TatI	WGTACW	1 cut(s)	424
TauI	GCSGC	8 cut(s)	93, 189, 192, 195, 198, 201, 546, 575
TfiI	GAWTC	6 cut(s)	14, 119, 125, 682, 695, 1564
Tru1I	TTAA	7 cut(s)	351, 464, 482, 486, 749, 866, 1154
Tru9I	TTAA	7 cut(s)	351, 464, 482, 486, 749, 866, 1154
TseFI	GTSAC	1 cut(s)	1301
TseI	GCWGC	2 cut(s)	620, 1171
Tsp45I	GTSAC	1 cut(s)	1301
TspDTI	ATGAA	6 cut(s)	482, 626, 1250, 1349, 1495, 1577
TspGWI	ACGGA	3 cut(s)	232, 319, 1320
VneI	GTGCAC	1 cut(s)	767
XapI	RAATTY	4 cut(s)	311, 460, 1036, 1466
XceI	RCATGY	3 cut(s)	936, 1129, 1255
XspI	CTAG	3 cut(s)	828, 915, 1343
ZrmI	AGTACT	1 cut(s)	426
Zsp2I	ATGCAT	2 cut(s)	1127, 1373

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.