Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0010352

Physical Location & Seq

Genomic Coordinates Forward (+)

17767335 .. 17772738

Gene Structure 5404 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1219900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2256 bp

ATGGCTGAGGATTTCTCCAAAGCCGTGGATGACGGTCTCAAGCTCTCCAAACGACTATACTTCGGGAAGGATCGAGCGGTGTCGCCGCCGAAGCCGCCGCCGTCGATGGACAAAGCGACGTCGTGCGCGTTCCTCCCTAAGGCGCCGATGGTCTACGCGGTCATCTCCGACCCGGCCATCGTCGACAACCCGGACATCCCCAGCTACCAGCCCCACGTGCACGGAAGGTGTGACCCCCCGGCCCTCATTCCGCTGACGATGAACCGGGTCGAGCTCGAGGTCGATTGCTACATGGACACCGCCTTTATTCGGGTTTCCGGGTCGTGGAGGGTCCATTGCGTAAAGGCTAGCAAGAGCTGCGGTTGCCGCGTTGCCATTCCTATGGGTGAACAGGGTTCAATTCTAGGTGTTGAGGTCGACGTAAACGGTAAATCGTACCATACTCAACTCAGTCATTTGGAAGATGAGAAGGAGATGGAGAAGATGGCCAATGCAGAGGGAGGCTTTCTCAGAGTCAAACCTCTTATATTCACATTAACAACACCTCAGGTCAACGGAGGAGCCAATATCTCAATCAAAATGAGTTGGTCTCAGAAATTGTCGTATTCAAATGGTGATTTCTCTTTGAATGTACCATTCACTTTTCCGGAGTATGTCATCCCCCCGGGAAGGAAGTACCTGAAAAAAGAAAAGATCCAATTAAACGTGAACTCTTGCGTTGGAACTGAAGTTTCGTGCAAGACAGTTAGTCATTCTTTGAAGGAATTACGGGGAATAAGCCAAGTTGGGAAATTGGGTTTTCTGTACGAGGCAGATGTTCTGAATTGGTCAAATACCAACTTTAATTTCTCGTACACGGTCTCGTCGAGTCATACACTTGGTACTGTTCTCGTGCAGTCACCGCCCACGCATGACATTGATCAAAGAGAAATCTTCTGTGTCTATCTTCTACCAGGAAACGAGCAGAGCAGGAAGGTTTTCAGAAGGGATATAGTGTTTGTTGTTGATATTAGTGGAAGTATGCAGGGAAAGCCACTTGATGATGTTAAGAATGTACTATCTGCAGCCCTCTCAAAGCTTCATCCAGAAGATTCGTTTTGCATTATCGCTTTTAATGGACAGACTTTTTTATCTTCCACATCAATGAAATCGGTTACCAAGGAGGATGTTGAAAGGGCCATTGAGTGGATTGGCATAAATTGTGTTGCTGGCGGCGATACAGATATGTTGCCTCCCCTTAACCAAGCAATAGAGATGCTATCCAATACTCGGGGTTCATTTCCTATCATCTTCCTTGTTACTGATGGGTCTGTTCAAGATGAGAGGCACATTTGTGATGTAATGAAGAAGCGTCTTACAGAAGCAGGTCCTATAGCTCCACGTATATACACTTTTGGAATAGGTACATTCTGCAACCATTATTTCCTGCGAATGCTTGCAATGATTGGCAGGGGCCAATATGATGCTGCGTACGATATAGATTTGGTCGAGTCTCGAATGCAGAGCTTATTTACCAGAGCCTCATCTGTGATTCTTACAAATATAACCCTTGAAACACTGGATGATCTTGTTGATGTTGAGGTATTCCCTTCCCATATTCCAGACCTCTCATCTGAGAGTCCATTAACCGTATCTGGCAAATTCCGAGGAAAGTTTCCCAGCTCACTTAAAGCTAAAGGCCTTCTACCTGATATGTCTAATATCGAGATCGATTTGAAGTTACAAGATGCAAAGGACATACCTCTTGACAGGGTATGTGCAAAAGGACAGATTGAGTTGTTGACTGCTTGGGCATGGTATGGAGATAACAAAGAGCTTCAAGATAAGGTAGCTGAACTGAGCATGCAGACCAATGTCGTGAGTGAGTATACATGCATGGTGATACTTCACAAAGAACTATCTGGACCACAAGAGACATCAAAGAAAAGCCGCACAAACAAGACGAAAGACTCTGAAAAACCGACGTTGCTTCCAACACTATCCGTTGGCTTTGGCAACTTAGTGGCAACAGCCGAGAACATTCCTCCTGGATCCGAAGAACCAAAACTCCCGGAAGCTGCTGAAATTTTTGTGAGAGCAGCTTCGAGTTGCTGTGGCTCAATGTGGAACAGATGTTGTTGCATGGCCTGCATCCAGTGTTGCTTCAAGGTAAATCCTCAATGTGCAAACGTATTCACTCAGCTCTGCACTGGTCTGGCATGTGCTGGCTGCCTCAACTGCTGTGTCTTCTGCTGTGGCGGAGATGGTGGATCGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

VWA_3

Protein Analysis

752

Amino Acids

82.44

Weight (kDa)

5.82

Isoelectric Point (pI)

40.25

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
VWA_3	PF13768	330 - 488	3e-15	von Willebrand factor type A domain
VWA	PF00092	330 - 480	5.9e-10	von Willebrand factor type A domain
VWA_2	PF13519	331 - 436	1.3e-10	von Willebrand factor type A domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010352)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G19110
fragaria_vesca	FvH4_1g08960 FvH4_1g08960 FvH4_1g08960 FvH4_1g08960
malus_domestica	MD02G1095100.v1.1 MD15G1219900.v1.1
prunus_persica	Prupe.7G196100_v2.0.a1 Prupe.7G196100_v2.0.a1
pyrus_communis	pycom02g07510 pycom15g19500
rosa_chinensis	RchiOBHm_Chr2g0095411
rosa_laevigata	RLG00000016563
rosa_multiflora	Rmu_ssc0000183.1_g000044
rosa_roxburghii	Rroxscaffold_2G00146390
rosa_rugosa	Rorug02G0050700
rosa_samantha	Rh2AG098800 Rh2BG100000 Rh2CG102000 Rh2DG099600
rosa_wichuraiana	Rw2G007460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	122
Acc36I	ACCTGC	1 cut(s)	1355
AccB1I	GGYRCC	1 cut(s)	142
AccB7I	CCANNNNNTGG	1 cut(s)	1185
AccBSI	CCGCTC	1 cut(s)	77
AccI	GTMKAC	4 cut(s)	153, 183, 417, 1868
AccII	CGCG	3 cut(s)	128, 158, 369
AccIII	TCCGGA	1 cut(s)	646
AclWI	GGATC	4 cut(s)	78, 688, 2025, 2038
AcoI	YGGCCR	2 cut(s)	174, 486
AcsI	RAATTY	2 cut(s)	1640, 2064
AcuI	CTGAAG	1 cut(s)	747
AcvI	CACGTG	1 cut(s)	217
AcyI	GRCGYC	2 cut(s)	119, 143
AfaI	GTAC	9 cut(s)	437, 633, 677, 806, 854, 883, 1056, 1405, 1472
AfiI	CCNNNNNNNGG	6 cut(s)	139, 309, 324, 669, 1185, 1269
AhdI	GACNNNNNGTC	1 cut(s)	747
AjnI	CCWGG	2 cut(s)	952, 2026
AjuI	GAANNNNNNNTTGG	2 cut(s)	830, 862
AleI	CACNNNNGTG	1 cut(s)	1332
AloI	GAACNNNNNNTCC	2 cut(s)	2031, 2063
Alw21I	GWGCWC	2 cut(s)	222, 276
Alw26I	GTCTC	5 cut(s)	41, 594, 865, 1497, 1907
Alw44I	GTGCAC	1 cut(s)	218
AlwI	GGATC	4 cut(s)	78, 688, 2025, 2038
Ama87I	CYCGRG	3 cut(s)	275, 664, 1269
Aor13HI	TCCGGA	1 cut(s)	646
AoxI	GGCC	7 cut(s)	174, 240, 486, 1176, 1453, 1678, 2124
ApaLI	GTGCAC	1 cut(s)	218
ApeKI	GCWGC	6 cut(s)	357, 1064, 1466, 2057, 2078, 2208
ApoI	RAATTY	2 cut(s)	1640, 2064
Asp700I	GAANNNNTTC	1 cut(s)	977
AspLEI	GCGC	2 cut(s)	128, 145
AspS9I	GGNCC	6 cut(s)	241, 331, 1176, 1367, 1453, 1904
AsuC2I	CCSGG	8 cut(s)	173, 191, 239, 266, 319, 665, 666, 2051
AsuHPI	GGTGA	4 cut(s)	398, 626, 891, 1891
AsuNHI	GCTAGC	1 cut(s)	347
AvaI	CYCGRG	3 cut(s)	275, 664, 1269
AvaII	GGWCC	3 cut(s)	331, 1367, 1904
AxyI	CCTNAGG	2 cut(s)	138, 546
BaeGI	GKGCMC	1 cut(s)	222
BalI	TGGCCA	1 cut(s)	488
BamHI	GGATCC	1 cut(s)	2030
BanI	GGYRCC	1 cut(s)	142
BanII	GRGCYC	1 cut(s)	276
BauI	CACGAG	1 cut(s)	890
BbrPI	CACGTG	1 cut(s)	217
BbsI	GAAGAC	1 cut(s)	2218
Bbv12I	GWGCWC	2 cut(s)	222, 276
BbvCI	CCTCAGC	1 cut(s)	6
BbvI	GCAGC	6 cut(s)	344, 1076, 1453, 2044, 2090, 2195
BccI	CCATC	7 cut(s)	100, 142, 185, 469, 478, 1298, 2237
BceAI	ACGGC	2 cut(s)	8, 85
BciT130I	CCWGG	2 cut(s)	954, 2028
BclI	TGATCA	1 cut(s)	919
BcnI	CCSGG	8 cut(s)	173, 191, 239, 266, 319, 665, 666, 2051
BcoDI	GTCTC	5 cut(s)	41, 594, 865, 1497, 1907
BfaI	CTAG	2 cut(s)	348, 404
BfmI	CTRYAG	2 cut(s)	1062, 1371
BfoI	RGCGCY	1 cut(s)	146
BfuAI	ACCTGC	1 cut(s)	1355
Bme18I	GGWCC	3 cut(s)	331, 1367, 1904
BmeRI	GACNNNNNGTC	1 cut(s)	747
BmeT110I	CYCGRG	3 cut(s)	275, 664, 1269
BmgT120I	GGNCC	6 cut(s)	241, 331, 1176, 1367, 1453, 1904
BmiI	GGNNCC	5 cut(s)	144, 332, 562, 1454, 2032
BmsI	GCATC	4 cut(s)	1245, 1453, 1717, 2139
BmtI	GCTAGC	1 cut(s)	351
BoxI	GACNNNNGTC	1 cut(s)	1853
BpiI	GAAGAC	1 cut(s)	2218
BplI	GAGNNNNNCTC	5 cut(s)	31, 492, 524, 574, 606
Bpu10I	CCTNAGC	1 cut(s)	6
BpuEI	CTTGAG	1 cut(s)	23
BpuMI	CCSGG	8 cut(s)	173, 191, 239, 266, 319, 665, 666, 2051
Bsa29I	ATCGAT	1 cut(s)	1710
BsaAI	YACGTR	2 cut(s)	217, 1382
BsaHI	GRCGYC	2 cut(s)	119, 143
BsaI	GGTCTC	3 cut(s)	41, 594, 865
BsaJI	CCNNGG	6 cut(s)	24, 237, 663, 664, 1158, 1645
BsaWI	WCCGGW	1 cut(s)	646
BsaXI	ACNNNNNCTCC	2 cut(s)	2031, 2061
Bsc4I	CCNNNNNNNGG	6 cut(s)	139, 309, 324, 669, 1185, 1269
Bse1I	ACTGG	3 cut(s)	1563, 2134, 2194
Bse21I	CCTNAGG	2 cut(s)	138, 546
Bse3DI	GCAATG	2 cut(s)	334, 1446
BseAI	TCCGGA	1 cut(s)	646
BseBI	CCWGG	2 cut(s)	954, 2028
BseCI	ATCGAT	1 cut(s)	1710
BseDI	CCNNGG	6 cut(s)	24, 237, 663, 664, 1158, 1645
BseGI	GGATG	7 cut(s)	34, 195, 657, 1081, 1171, 1567, 2130
BseLI	CCNNNNNNNGG	6 cut(s)	139, 309, 324, 669, 1185, 1269
BseMI	GCAATG	2 cut(s)	334, 1446
BseMII	CTCAG	7 cut(s)	463, 523, 560, 605, 1605, 1829, 2192
BseNI	ACTGG	3 cut(s)	1563, 2134, 2194
BseRI	GAGGAG	1 cut(s)	573
BseSI	GKGCMC	1 cut(s)	222
BseXI	GCAGC	6 cut(s)	344, 1076, 1453, 2044, 2090, 2195
BseYI	CCCAGC	2 cut(s)	200, 1658
BsgI	GTGCAG	2 cut(s)	914, 2170
Bsh1236I	CGCG	3 cut(s)	128, 158, 369
BshFI	GGCC	7 cut(s)	176, 242, 488, 1178, 1455, 1680, 2126
BshNI	GGYRCC	1 cut(s)	142
BshVI	ATCGAT	1 cut(s)	1710
BsiHKAI	GWGCWC	2 cut(s)	222, 276
BsiHKCI	CYCGRG	3 cut(s)	275, 664, 1269
BsiSI	CCGG	8 cut(s)	173, 191, 239, 265, 318, 647, 665, 2051
BsiWI	CGTACG	1 cut(s)	1470
BslI	CCNNNNNNNGG	6 cut(s)	139, 309, 324, 669, 1185, 1269
BsmAI	GTCTC	5 cut(s)	41, 594, 865, 1497, 1907
BsmI	GAATGC	2 cut(s)	1437, 1503
BsnI	GGCC	7 cut(s)	176, 242, 488, 1178, 1455, 1680, 2126
Bso31I	GGTCTC	3 cut(s)	41, 594, 865
BsoBI	CYCGRG	3 cut(s)	275, 664, 1269
Bsp1286I	GDGCHC	2 cut(s)	222, 276
Bsp13I	TCCGGA	1 cut(s)	646
Bsp143I	GATC	7 cut(s)	70, 693, 919, 1564, 1707, 2030, 2249
BspANI	GGCC	7 cut(s)	176, 242, 488, 1178, 1455, 1680, 2126
BspCNI	CTCAG	7 cut(s)	462, 522, 559, 604, 1606, 1830, 2191
BspDI	ATCGAT	1 cut(s)	1710
BspEI	TCCGGA	1 cut(s)	646
BspFNI	CGCG	3 cut(s)	128, 158, 369
BspLI	GGNNCC	5 cut(s)	144, 332, 562, 1454, 2032
BspMAI	CTGCAG	1 cut(s)	1066
BspMI	ACCTGC	1 cut(s)	1355
BspOI	GCTAGC	1 cut(s)	351
BspPI	GGATC	4 cut(s)	78, 688, 2025, 2038
BspT107I	GGYRCC	1 cut(s)	142
BspTNI	GGTCTC	3 cut(s)	41, 594, 865
BsrBI	CCGCTC	1 cut(s)	77
BsrDI	GCAATG	2 cut(s)	334, 1446
BsrI	ACTGG	3 cut(s)	1563, 2134, 2194
BssECI	CCNNGG	6 cut(s)	24, 237, 663, 664, 1158, 1645
BssMI	GATC	7 cut(s)	70, 693, 919, 1564, 1707, 2030, 2249
BssNAI	GTATAC	1 cut(s)	1869
BssNI	GRCGYC	2 cut(s)	119, 143
BssSI	CACGAG	1 cut(s)	890
BssT1I	CCWWGG	1 cut(s)	1158
Bst1107I	GTATAC	1 cut(s)	1869
Bst2BI	CACGAG	1 cut(s)	890
Bst2UI	CCWGG	2 cut(s)	954, 2028
Bst4CI	ACNGT	6 cut(s)	35, 428, 745, 859, 886, 1630
BstACI	GRCGYC	2 cut(s)	119, 143
BstAPI	GCANNNNNTGC	2 cut(s)	357, 2127
BstBAI	YACGTR	2 cut(s)	217, 1382
BstC8I	GCNNGC	6 cut(s)	349, 1210, 1437, 1844, 2128, 2206
BstDSI	CCRYGG	1 cut(s)	24
BstEII	GGTNACC	1 cut(s)	1153
BstENI	CCTNNNNNAGG	1 cut(s)	137
BstF5I	GGATG	7 cut(s)	34, 195, 657, 1081, 1171, 1567, 2130
BstFNI	CGCG	3 cut(s)	128, 158, 369
BstH2I	RGCGCY	1 cut(s)	146
BstHHI	GCGC	2 cut(s)	128, 145
BstKTI	GATC	7 cut(s)	73, 696, 922, 1567, 1710, 2033, 2252
BstMAI	GTCTC	5 cut(s)	41, 594, 865, 1497, 1907
BstMBI	GATC	7 cut(s)	70, 693, 919, 1564, 1707, 2030, 2249
BstNI	CCWGG	2 cut(s)	954, 2028
BstNSI	RCATGY	3 cut(s)	1846, 1875, 2202
BstPAI	GACNNNNGTC	1 cut(s)	1853
BstPI	GGTNACC	1 cut(s)	1153
BstSFI	CTRYAG	2 cut(s)	1062, 1371
BstSLI	GKGCMC	1 cut(s)	222
BstUI	CGCG	3 cut(s)	128, 158, 369
BstV1I	GCAGC	6 cut(s)	344, 1076, 1453, 2044, 2090, 2195
BstV2I	GAAGAC	1 cut(s)	2218
BstX2I	RGATCY	2 cut(s)	693, 2030
BstXI	CCANNNNNNTGG	2 cut(s)	25, 382
BstYI	RGATCY	2 cut(s)	693, 2030
BstZ17I	GTATAC	1 cut(s)	1869
Bsu15I	ATCGAT	1 cut(s)	1710
Bsu36I	CCTNAGG	2 cut(s)	138, 546
BsuRI	GGCC	7 cut(s)	176, 242, 488, 1178, 1455, 1680, 2126
BsuTUI	ATCGAT	1 cut(s)	1710
BtgI	CCRYGG	1 cut(s)	24
BtsCI	GGATG	7 cut(s)	34, 195, 657, 1081, 1171, 1567, 2130
BtsIMutI	CAGTG	3 cut(s)	1556, 2141, 2187
BveI	ACCTGC	1 cut(s)	1355
Cac8I	GCNNGC	6 cut(s)	349, 1210, 1437, 1844, 2128, 2206
CfoI	GCGC	2 cut(s)	128, 145
Cfr13I	GGNCC	6 cut(s)	241, 331, 1176, 1367, 1453, 1904
Cfr9I	CCCGGG	1 cut(s)	664
ClaI	ATCGAT	1 cut(s)	1710
CseI	GACGC	1 cut(s)	1340
Csp6I	GTAC	9 cut(s)	436, 632, 676, 805, 853, 882, 1055, 1404, 1471
CviAII	CATG	8 cut(s)	292, 911, 1794, 1843, 1872, 1876, 2122, 2199
CviQI	GTAC	9 cut(s)	436, 632, 676, 805, 853, 882, 1055, 1404, 1471
DinI	GGCGCC	1 cut(s)	144
DpnI	GATC	7 cut(s)	72, 695, 921, 1566, 1709, 2032, 2251
DpnII	GATC	7 cut(s)	70, 693, 919, 1564, 1707, 2030, 2249
DriI	GACNNNNNGTC	1 cut(s)	747
EaeI	YGGCCR	2 cut(s)	174, 486
Eam1105I	GACNNNNNGTC	1 cut(s)	747
EciI	GGCGGA	1 cut(s)	2253
Ecl136II	GAGCTC	1 cut(s)	274
Eco130I	CCWWGG	1 cut(s)	1158
Eco147I	AGGCCT	1 cut(s)	1680
Eco24I	GRGCYC	1 cut(s)	276
Eco31I	GGTCTC	3 cut(s)	41, 594, 865
Eco47I	GGWCC	3 cut(s)	331, 1367, 1904
Eco53kI	GAGCTC	1 cut(s)	274
Eco57I	CTGAAG	1 cut(s)	747
Eco72I	CACGTG	1 cut(s)	217
Eco81I	CCTNAGG	2 cut(s)	138, 546
Eco88I	CYCGRG	3 cut(s)	275, 664, 1269
Eco91I	GGTNACC	1 cut(s)	1153
EcoICRI	GAGCTC	1 cut(s)	274
EcoNI	CCTNNNNNAGG	1 cut(s)	137
EcoO109I	RGGNCCY	1 cut(s)	1367
EcoO65I	GGTNACC	1 cut(s)	1153
EcoRII	CCWGG	2 cut(s)	952, 2026
EcoT14I	CCWWGG	1 cut(s)	1158
EcoT22I	ATGCAT	1 cut(s)	1877
EcoT38I	GRGCYC	1 cut(s)	276
EgeI	GGCGCC	1 cut(s)	144
EheI	GGCGCC	1 cut(s)	144
ErhI	CCWWGG	1 cut(s)	1158
FaeI	CATG	8 cut(s)	295, 914, 1797, 1846, 1875, 1879, 2125, 2202
FatI	CATG	8 cut(s)	291, 910, 1793, 1842, 1871, 1875, 2121, 2198
FbaI	TGATCA	1 cut(s)	919
FblI	GTMKAC	4 cut(s)	153, 183, 417, 1868
FokI	GGATG	7 cut(s)	41, 182, 644, 1068, 1178, 1574, 2117
FriOI	GRGCYC	1 cut(s)	276
FspBI	CTAG	2 cut(s)	348, 404
GlaI	GCGC	2 cut(s)	127, 144
GsaI	CCCAGC	2 cut(s)	204, 1662
HaeII	RGCGCY	1 cut(s)	146
HaeIII	GGCC	7 cut(s)	176, 242, 488, 1178, 1455, 1680, 2126
HapII	CCGG	8 cut(s)	173, 191, 239, 265, 318, 647, 665, 2051
HgaI	GACGC	1 cut(s)	1340
HhaI	GCGC	2 cut(s)	128, 145
Hin1I	GRCGYC	2 cut(s)	119, 143
Hin1II	CATG	8 cut(s)	295, 914, 1797, 1846, 1875, 1879, 2125, 2202
Hin6I	GCGC	2 cut(s)	126, 143
HinP1I	GCGC	2 cut(s)	126, 143
HincII	GTYRAC	4 cut(s)	184, 418, 553, 1782
HindII	GTYRAC	4 cut(s)	184, 418, 553, 1782
HindIII	AAGCTT	1 cut(s)	1076
HinfI	GANTC	7 cut(s)	513, 868, 1091, 1490, 1531, 1618, 1949
HpaII	CCGG	8 cut(s)	173, 191, 239, 265, 318, 647, 665, 2051
HphI	GGTGA	4 cut(s)	398, 626, 891, 1891
Hpy188I	TCNGA	9 cut(s)	169, 512, 594, 822, 983, 1615, 1646, 1954, 2035
Hpy99I	CGWCG	7 cut(s)	106, 121, 124, 185, 422, 868, 1966
HpyAV	CCTTC	9 cut(s)	61, 219, 463, 663, 754, 967, 978, 1599, 1691
HpyCH4III	ACNGT	6 cut(s)	35, 428, 745, 859, 886, 1630
HpyCH4IV	ACGT	7 cut(s)	119, 216, 420, 705, 1381, 1964, 2169
HpySE526I	ACGT	7 cut(s)	119, 216, 420, 705, 1381, 1964, 2169
Hsp92I	GRCGYC	2 cut(s)	119, 143
Hsp92II	CATG	8 cut(s)	295, 914, 1797, 1846, 1875, 1879, 2125, 2202
HspAI	GCGC	2 cut(s)	126, 143
KasI	GGCGCC	1 cut(s)	142
Kpn2I	TCCGGA	1 cut(s)	646
Ksp22I	TGATCA	1 cut(s)	919
Kzo9I	GATC	7 cut(s)	70, 693, 919, 1564, 1707, 2030, 2249
LmnI	GCTCC	2 cut(s)	560, 1381
Lsp1109I	GCAGC	6 cut(s)	344, 1076, 1453, 2044, 2090, 2195
LweI	GCATC	4 cut(s)	1245, 1453, 1717, 2139
MaeI	CTAG	2 cut(s)	348, 404
MaeII	ACGT	7 cut(s)	119, 216, 420, 705, 1381, 1964, 2169
MaeIII	GTNAC	5 cut(s)	230, 897, 1153, 1297, 1719
MalI	GATC	7 cut(s)	72, 695, 921, 1566, 1709, 2032, 2251
MbiI	CCGCTC	1 cut(s)	77
MboI	GATC	7 cut(s)	70, 693, 919, 1564, 1707, 2030, 2249
MflI	RGATCY	2 cut(s)	693, 2030
MhlI	GDGCHC	2 cut(s)	222, 276
MlsI	TGGCCA	1 cut(s)	488
MluNI	TGGCCA	1 cut(s)	488
Mly113I	GGCGCC	1 cut(s)	143
MlyI	GAGTC	5 cut(s)	522, 877, 1499, 1627, 1943
MmeI	TCCRAC	3 cut(s)	192, 700, 1997
Mox20I	TGGCCA	1 cut(s)	488
Mph1103I	ATGCAT	1 cut(s)	1877
MroI	TCCGGA	1 cut(s)	646
MroXI	GAANNNNTTC	1 cut(s)	977
MscI	TGGCCA	1 cut(s)	488
MseI	TTAA	8 cut(s)	536, 701, 843, 1047, 1113, 1239, 1625, 1668
MslI	CAYNNNNRTG	3 cut(s)	380, 1142, 1332
Msp20I	TGGCCA	1 cut(s)	488
MspA1I	CMGCKG	1 cut(s)	253
MspI	CCGG	8 cut(s)	173, 191, 239, 265, 318, 647, 665, 2051
Mva1269I	GAATGC	2 cut(s)	1437, 1503
MvaI	CCWGG	2 cut(s)	954, 2028
MvnI	CGCG	3 cut(s)	128, 158, 369
NarI	GGCGCC	1 cut(s)	143
NciI	CCSGG	8 cut(s)	173, 191, 239, 266, 319, 665, 666, 2051
NdeII	GATC	7 cut(s)	70, 693, 919, 1564, 1707, 2030, 2249
NheI	GCTAGC	1 cut(s)	347
NlaIII	CATG	8 cut(s)	295, 914, 1797, 1846, 1875, 1879, 2125, 2202
NlaIV	GGNNCC	5 cut(s)	144, 332, 562, 1454, 2032
NmeAIII	GCCGAG	1 cut(s)	2038
NmuCI	GTSAC	2 cut(s)	230, 897
NsiI	ATGCAT	1 cut(s)	1877
NspI	RCATGY	3 cut(s)	1846, 1875, 2202
OliI	CACNNNNGTG	1 cut(s)	1332
PaeI	GCATGC	1 cut(s)	1846
PaeR7I	CTCGAG	1 cut(s)	275
PceI	AGGCCT	1 cut(s)	1680
PcsI	WCGNNNNNNNCGW	2 cut(s)	125, 863
PctI	GAATGC	2 cut(s)	1437, 1503
PdmI	GAANNNNTTC	1 cut(s)	977
PfeI	GAWTC	2 cut(s)	1091, 1531
Pfl23II	CGTACG	1 cut(s)	1470
PflMI	CCANNNNNTGG	1 cut(s)	1185
PfoI	TCCNGGA	2 cut(s)	2026, 2049
PleI	GAGTC	5 cut(s)	521, 876, 1498, 1626, 1943
PluTI	GGCGCC	1 cut(s)	146
PmaCI	CACGTG	1 cut(s)	217
PmlI	CACGTG	1 cut(s)	217
PpsI	GAGTC	5 cut(s)	521, 876, 1498, 1626, 1943
Ppu21I	YACGTR	2 cut(s)	217, 1382
PpuMI	RGGWCCY	1 cut(s)	1367
PshAI	GACNNNNGTC	1 cut(s)	1853
Psp124BI	GAGCTC	1 cut(s)	276
Psp5II	RGGWCCY	1 cut(s)	1367
Psp6I	CCWGG	2 cut(s)	952, 2026
PspCI	CACGTG	1 cut(s)	217
PspEI	GGTNACC	1 cut(s)	1153
PspFI	CCCAGC	2 cut(s)	200, 1658
PspGI	CCWGG	2 cut(s)	952, 2026
PspLI	CGTACG	1 cut(s)	1470
PspN4I	GGNNCC	5 cut(s)	144, 332, 562, 1454, 2032
PspPI	GGNCC	6 cut(s)	241, 331, 1176, 1367, 1453, 1904
PspPPI	RGGWCCY	1 cut(s)	1367
PspXI	VCTCGAGB	1 cut(s)	275
PstI	CTGCAG	1 cut(s)	1066
PsuI	RGATCY	2 cut(s)	693, 2030
RsaI	GTAC	9 cut(s)	437, 633, 677, 806, 854, 883, 1056, 1405, 1472
RsaNI	GTAC	9 cut(s)	436, 632, 676, 805, 853, 882, 1055, 1404, 1471
RseI	CAYNNNNRTG	3 cut(s)	380, 1142, 1332
SacI	GAGCTC	1 cut(s)	276
SalI	GTCGAC	2 cut(s)	182, 416
SaqAI	TTAA	8 cut(s)	536, 701, 843, 1047, 1113, 1239, 1625, 1668
Sau3AI	GATC	7 cut(s)	70, 693, 919, 1564, 1707, 2030, 2249
Sau96I	GGNCC	6 cut(s)	241, 331, 1176, 1367, 1453, 1904
SchI	GAGTC	5 cut(s)	522, 877, 1499, 1627, 1943
SduI	GDGCHC	2 cut(s)	222, 276
SfaNI	GCATC	4 cut(s)	1245, 1453, 1717, 2139
SfcI	CTRYAG	2 cut(s)	1062, 1371
SfoI	GGCGCC	1 cut(s)	144
Sfr274I	CTCGAG	1 cut(s)	275
SinI	GGWCC	3 cut(s)	331, 1367, 1904
SlaI	CTCGAG	1 cut(s)	275
SmaI	CCCGGG	1 cut(s)	666
SmiMI	CAYNNNNRTG	3 cut(s)	380, 1142, 1332
SmlI	CTYRAG	2 cut(s)	38, 275
SmoI	CTYRAG	2 cut(s)	38, 275
SphI	GCATGC	1 cut(s)	1846
SseBI	AGGCCT	1 cut(s)	1680
SspDI	GGCGCC	1 cut(s)	142
SspMI	CTAG	2 cut(s)	348, 404
SstI	GAGCTC	1 cut(s)	276
StuI	AGGCCT	1 cut(s)	1680
StyI	CCWWGG	1 cut(s)	1158
TaaI	ACNGT	6 cut(s)	35, 428, 745, 859, 886, 1630
TaiI	ACGT	7 cut(s)	122, 219, 423, 708, 1384, 1967, 2172
TatI	WGTACW	1 cut(s)	1054
TauI	GCSGC	6 cut(s)	88, 97, 100, 369, 1215, 1932
TfiI	GAWTC	2 cut(s)	1091, 1531
Tru1I	TTAA	8 cut(s)	536, 701, 843, 1047, 1113, 1239, 1625, 1668
Tru9I	TTAA	8 cut(s)	536, 701, 843, 1047, 1113, 1239, 1625, 1668
TscAI	CASTG	3 cut(s)	1563, 2141, 2194
TseFI	GTSAC	2 cut(s)	230, 897
TseI	GCWGC	6 cut(s)	357, 1064, 1466, 2057, 2078, 2208
Tsp45I	GTSAC	2 cut(s)	230, 897
TspDTI	ATGAA	5 cut(s)	275, 1070, 1160, 1266, 1358
TspGWI	ACGGA	3 cut(s)	237, 570, 1972
TspMI	CCCGGG	1 cut(s)	664
TspRI	CASTG	3 cut(s)	1563, 2141, 2194
Van91I	CCANNNNNTGG	1 cut(s)	1185
VneI	GTGCAC	1 cut(s)	218
VpaK11BI	GGWCC	3 cut(s)	331, 1367, 1904
XagI	CCTNNNNNAGG	1 cut(s)	137
XapI	RAATTY	2 cut(s)	1640, 2064
XceI	RCATGY	3 cut(s)	1846, 1875, 2202
XhoI	CTCGAG	1 cut(s)	275
XmaI	CCCGGG	1 cut(s)	664
XmiI	GTMKAC	4 cut(s)	153, 183, 417, 1868
XmnI	GAANNNNTTC	1 cut(s)	977
XspI	CTAG	2 cut(s)	348, 404
ZraI	GACGTC	1 cut(s)	120
Zsp2I	ATGCAT	1 cut(s)	1877

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.