Rroxscaffold_2G00146390

Inter-alpha-trypsin inhibitor heavy

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0010352
Physical Location & Seq
Reverse (-)
84294112 .. 84302970
8859 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00146390.1

Sequence Viewer

Length: 3306 bp
ATGGCCGAGGAGTTCTCCAAAGCCGTCGACGACGGTCTCCGGCTCTCGAAACGACTATACTTCGGGAAGGATCGAGCCGTGGCGCCGCCGAGGCCGCCGCCGGTCATGGACAAGTCGGCGTCGTCGTCGGCGGCCGCTGGGTGCGCCTACCTCCCGACGTCTCCGATGGTGTACGCCGTGATATCGGACCCCAGGATCGTGGACAACCCGGACATCCCGAGCTACCAGCCGCACGTGCACGGCAGGTGCGACCCGCCGGCGCTCATTCCGCTTCCGATGAACAGGGTTGAGCTGGAGGTCGATTGCTTCATGGACACCGCCTTTATTCGGGTCACCGGGTCGTGGAGGGTCCATTGCGTCAAGAGCTCCAGGAGCTCTGGCTGCCGGATTGCCATCCCTATGGGCGAACAGGGTTCAATTCTAGGTGTTGAGGTCGACATTTCTGGTAAATCATTCTTTACTCAGTTAAGTCAATTGAAAGATGACACGGATATGAGCAAGGTTCCCCAAGGAGAAGGAGGCTTTCTCAATGTTAAACCTCATATATTCACCTTGACAACTCCACCGGTTGATGGGGGAGTCAATCTCACAATTAAAATGAGTTGGTCTCAGAAGTTATTGTATCAAAATGGAGAGTTCTCTTTAGATATACCGTTCACATTTCCGGAGTTTGTCATTCCTCCCGGCAAGAAGTACCTGAAAAAGGAAAAGATTGAATTGAATGTGAACTCTGGTCTGGGAACTGAAATTTTATTCAAGGGAACTAGTCATCTTTTGAAGGAAGTACGGAGCCAAGAAGGAAAATTGGGGTTTAAATACGAGGGAAATGTTGTCAACTGGTCAACTACCGACTTCCACTTCTCATATGCGGTCACTTCAAGCCAAATACATGGCACTGTTATCACGCAGTCACCTTCCAAGGATGATGTTGATCAAAGAGAGATTTTCTGTGCCTATCTTCTTCCTGGAAACCAGCGAAGTGGAAAGGTTGGTGTTATGTTAAATGGAACTTCTCAGGGTTTCAGAAGGAATGTAGTGGTTGTTGTTGATATAAGTGGAAGTATGCAGGGAAAGCCACTTGAAGATACTCAGAATGCACTGTCTGAAGCCCTCTCTAAACTTGATCCAGAAGATTCATTCTGCATTATAGCTTTTAATGGACAGACTTATGTTTCATCTACATCATTGAAATTGGCTACCAAGGAGGCAGTCGAATCGGCCATTGAGTGGATTCGCATCAACTTTATTGCTGGCGGTGATACAAATATTATGCGTCCCCTTAACATGGCAATAGAGATGCTATCCAACTCTAGGGGTTCACTTCCTATCATATTTCTGGTTACTGATGGGGCAGTTGAAGATGAGAGACAAATATGCGATGAAATAAAAAAGCGTCTTGCAAGTGAGGATGCGATATCCCCTCGTATATACACTTTTGGAATAGGTTCGTTCTGCAACCATTATTTCCTGCGGATGCTATCAACAATTGGAAGGGGCCAATATGATGCTGCCTATGATATAGATTTGGTTCAGCCTCGAATACAGAACTTATTTGCGAGAGCTTCATCTGTGATTCTTACAAATATAACCCTTGAGACATTGGACGATCTTGATGATGTTGAGGTGCTCCCTTGCCATATTCCAGACCTCTCATTTGAGAGTCCATTAAGTGTATCTGGCAGATTCCGAGGCAAGTTACCTGAGACCTTCAAAGTTAAAGGATTCTCAGCTGATATGTCTAATATTGTGATCGATTTGAAAGTACAAGATGCAAAGGACATACCTCTTCACAGGGTATGTGCAAAAGGAGAGATTGAGTTACTGACTGCTCAGGCATGGCTGTCAGAGAACAAGCAGCTTGAGGATAAGGTAGCTAAGTTGAGTGTACACACCGGTGCTTTGAGCGAGTATACACGCATGGTCATATTTGAAAAAGAAGAAGCTCTGCAACAGATGGCCTCAAAGAAAAGCCCGAACAAGAAGAAGGATACCGAAACCTTGAAAATGATATTGCCACATAGTTTGTGCGTCGGCTTTGGAAACGTGGCCGCAACTTCGGACAACATTCCTCCAGGAGCCGAAGAACCAAAGCTTCCGGAAGCAGTTGAAATTTTCGCCAAAGCCGCTTCAGGCTGTTGTGGTCATCTCTACAAACCAGCCGTGATAACTGCAGCTTCCCTTACAGTTGTGGCACTCATTCTCTCCCTTTTTCTCATCCTTCGGCATCTCAGATCCTACAACAAGCCTTCAGAACAAATATGGGTGGTTGCTGTTGTTTTCATGGTACCTGTCTATGCTACTGACTCGTTATTAGAGCTTGCATTATTCATGAATGGCTATTACGCAGGAGGTGAGAGAAGAGTAGTAGAACTACTTGAGAATCAATCTGGAAAGCTCCTAAACAAAGCATTGGTAGAAGGGGAAGATGAGAATTGCAGGAAACCACACTGGTCTCTTCGCAACTTCTTCAGGCAACCAACTATTATTGGGAAAAATTTGCTCACTATAGTGAAATTCGGTCTTGTACAATATATGATTCTGAAGACAGTTTGTGCATTCCTAGCACTCGTGTTGGAAATCTTTGGCGTCTATGGTGATGGGAAATTCAAGTTTCACTACGGGTATCCATACATCACGATAGTACTGAATTTCAGCCAGATGTGGGCATTGTATTGCCTTGTGCAATTTTATAATGTGACTCATGAAAGACTTCAACCAATAAGGCCGCTTGCAAAGTTCATCAGCTTTAAGGCTATTGTGTTTGCGACTTGGTGGCAAGGTGTAGGTATTGCGCTGTTGTGTTATTTTGGTGTTCTTCCCAACCAGGAAAAACTACAAACAGGGTTGCAGGATTTCTTGATTTGTATAGAAATGGCAATTGCAGCTGTTGCACATGTGTTTGTCTTTTCTGCAGAACCTTACCACTACATTCCATCTTCTTCTGGGTATGAGAAGATCACCACTATTGAAACGACCAGTGAGGAAGCAGTGAAGTTGGAGGAAGGTGACAGTAGTACACAAGCTGTGGTTGAAACAAAAGAAACTCAGGTCGAGGCTCCAGGAACAAGTGTGACTGAGAGTGTTCAGGACATAGTGTTTGAAGGTGGTCATCATGTTGTCAAGGATGTTGTGTTGACCATAAATCAAGCAATAGGGCCTGTGGAGAAGGGTGTGACGAAGGGTGTGACAAAGATACAAGAAACCTTCCACAAAAGATCAGATGAGCATGACGATAAGTCGGAGGTTTCAGTTGAGGAGAGTATTGAAGTGACTCTTTCCGATGAAGAAACACATTTGGACCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VWA_3

Protein Analysis

1101

Amino Acids

121.95

Weight (kDa)

5.58

Isoelectric Point (pI)

39.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VWA PF00092 344 - 498 2.2e-12 von Willebrand factor type A domain
VWA_3 PF13768 344 - 502 2.6e-10 von Willebrand factor type A domain
VWA_2 PF13519 345 - 450 1.5e-11 von Willebrand factor type A domain
Solute_trans_a PF03619 721 - 974 2.1e-71 Organic solute transporter Ostalpha
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2691
AarI CACCTGC 1 cut(s) 234
AatII GACGTC 1 cut(s) 161
Acc36I ACCTGC 1 cut(s) 234
Acc65I GGTACC 1 cut(s) 2284
AccB1I GGYRCC 2 cut(s) 82, 2284
AccB7I CCANNNNNTGG 1 cut(s) 1227
AccI GTMKAC 3 cut(s) 27, 435, 1910
AccIII TCCGGA 2 cut(s) 664, 2095
AclWI GGATC 4 cut(s) 78, 203, 1118, 2226
AcoI YGGCCR 4 cut(s) 3, 132, 1218, 2046
AcsI RAATTY 6 cut(s) 747, 2109, 2494, 2513, 2603, 2647
AcuI CTGAAG 5 cut(s) 1125, 2112, 2232, 2452, 2561
AcvI CACGTG 1 cut(s) 235
AcyI GRCGYC 4 cut(s) 83, 119, 158, 2586
AfaI GTAC 9 cut(s) 173, 695, 786, 1764, 1887, 2286, 2526, 2643, 3016
AfiI CCNNNNNNNGG 8 cut(s) 327, 342, 572, 703, 1227, 1285, 1311, 2662
AflIII ACRYGT 1 cut(s) 2893
AgeI ACCGGT 2 cut(s) 565, 1892
AhdI GACNNNNNGTC 1 cut(s) 3235
AhlI ACTAGT 1 cut(s) 764
AjnI CCWGG 6 cut(s) 191, 368, 964, 2071, 2823, 3058
AleI CACNNNNGTG 2 cut(s) 1893, 2507
Alw21I GWGCWC 4 cut(s) 240, 368, 377, 1629
Alw26I GTCTC 7 cut(s) 41, 165, 612, 1360, 1589, 1697, 2457
Alw44I GTGCAC 1 cut(s) 236
AlwI GGATC 4 cut(s) 78, 203, 1118, 2226
Ama87I CYCGRG 1 cut(s) 217
Aor13HI TCCGGA 2 cut(s) 664, 2095
AoxI GGCC 9 cut(s) 3, 92, 132, 1218, 1495, 1956, 2046, 2723, 3155
ApaLI GTGCAC 1 cut(s) 236
ApeKI GCWGC 5 cut(s) 381, 1508, 1855, 2171, 2882
ApoI RAATTY 6 cut(s) 747, 2109, 2494, 2513, 2603, 2647
ArsI GACNNNNNNTTYG 4 cut(s) 1637, 1669, 3080, 3112
AsiGI ACCGGT 2 cut(s) 565, 1892
Asp700I GAANNNNTTC 2 cut(s) 1444, 2709
Asp718I GGTACC 1 cut(s) 2284
AspLEI GCGC 4 cut(s) 85, 146, 262, 2794
AspS9I GGNCC 5 cut(s) 187, 349, 1495, 3155, 3298
AsuC2I CCSGG 3 cut(s) 209, 337, 684
AsuHPI GGTGA 8 cut(s) 325, 541, 903, 1268, 2363, 2606, 2950, 3017
AvaI CYCGRG 1 cut(s) 217
AvaII GGWCC 3 cut(s) 187, 349, 3298
BaeGI GKGCMC 1 cut(s) 240
BanI GGYRCC 2 cut(s) 82, 2284
BanII GRGCYC 2 cut(s) 368, 377
BauI CACGAG 1 cut(s) 2567
BbrPI CACGTG 1 cut(s) 235
BbsI GAAGAC 1 cut(s) 2549
Bbv12I GWGCWC 4 cut(s) 240, 368, 377, 1629
BbvI GCAGC 5 cut(s) 368, 1495, 1867, 2183, 2894
BccI CCATC 7 cut(s) 160, 401, 566, 1340, 1948, 2591, 2941
BceAI ACGGC 5 cut(s) 8, 62, 161, 256, 2144
BcgI CGANNNNNNTGC 2 cut(s) 1197, 1231
BciT130I CCWGG 6 cut(s) 193, 370, 966, 2073, 2825, 3060
BciVI GTATCC 2 cut(s) 1981, 2634
BclI TGATCA 1 cut(s) 931
BcnI CCSGG 3 cut(s) 209, 337, 684
BcoDI GTCTC 7 cut(s) 41, 165, 612, 1360, 1589, 1697, 2457
BcuI ACTAGT 1 cut(s) 764
BfaI CTAG 4 cut(s) 422, 765, 1311, 2561
BfmI CTRYAG 3 cut(s) 2169, 2505, 2910
BfoI RGCGCY 2 cut(s) 86, 263
BfuAI ACCTGC 1 cut(s) 234
BfuI GTATCC 2 cut(s) 1981, 2634
BglI GCCNNNNNGGC 1 cut(s) 91
BmcAI AGTACT 1 cut(s) 2643
Bme1390I CCNGG 9 cut(s) 193, 209, 337, 370, 684, 966, 2073, 2825, 3060
Bme18I GGWCC 3 cut(s) 187, 349, 3298
BmeRI GACNNNNNGTC 1 cut(s) 3235
BmeT110I CYCGRG 1 cut(s) 217
BmgT120I GGNCC 5 cut(s) 187, 349, 1495, 3155, 3298
BmiI GGNNCC 9 cut(s) 84, 189, 350, 504, 791, 1496, 2077, 2286, 3057
BmrFI CCNGG 9 cut(s) 193, 209, 337, 370, 684, 966, 2073, 2825, 3060
BmsI GCATC 7 cut(s) 1245, 1287, 1399, 1464, 1495, 1759, 2233
BoxI GACNNNNGTC 1 cut(s) 33
BpiI GAAGAC 1 cut(s) 2549
BplI GAGNNNNNCTC 7 cut(s) 31, 510, 542, 570, 602, 592, 624
BpmI CTGGAG 4 cut(s) 314, 352, 2055, 3042
Bpu10I CCTNAGC 1 cut(s) 1830
BpuEI CTTGAG 3 cut(s) 1613, 1880, 2396
BpuMI CCSGG 3 cut(s) 209, 337, 684
Bsa29I ATCGAT 1 cut(s) 1752
BsaAI YACGTR 1 cut(s) 235
BsaBI GATNNNNATC 3 cut(s) 392, 930, 1235
BsaHI GRCGYC 4 cut(s) 83, 119, 158, 2586
BsaI GGTCTC 4 cut(s) 41, 612, 1697, 2457
BsaJI CCNNGG 8 cut(s) 6, 78, 89, 191, 508, 918, 1200, 1687
BsaWI WCCGGW 4 cut(s) 565, 664, 1892, 2095
Bsc4I CCNNNNNNNGG 8 cut(s) 327, 342, 572, 703, 1227, 1285, 1311, 2662
Bse118I RCCGGY 4 cut(s) 100, 256, 565, 1892
Bse1I ACTGG 3 cut(s) 842, 2453, 2976
Bse3DI GCAATG 1 cut(s) 352
Bse8I GATNNNNATC 3 cut(s) 392, 930, 1235
BseAI TCCGGA 2 cut(s) 664, 2095
BseBI CCWGG 6 cut(s) 193, 370, 966, 2073, 2825, 3060
BseCI ATCGAT 1 cut(s) 1752
BseDI CCNNGG 8 cut(s) 6, 78, 89, 191, 508, 918, 1200, 1687
BseGI GGATG 7 cut(s) 213, 393, 928, 1414, 1479, 2214, 3130
BseJI GATNNNNATC 3 cut(s) 392, 930, 1235
BseLI CCNNNNNNNGG 8 cut(s) 327, 342, 572, 703, 1227, 1285, 1311, 2662
BseMI GCAATG 1 cut(s) 352
BseNI ACTGG 3 cut(s) 842, 2453, 2976
BseRI GAGGAG 2 cut(s) 23, 3269
BseSI GKGCMC 1 cut(s) 240
BseX3I CGGCCG 1 cut(s) 132
BseXI GCAGC 5 cut(s) 368, 1495, 1867, 2183, 2894
BseYI CCCAGC 1 cut(s) 137
Bsh1285I CGRYCG 1 cut(s) 135
BshFI GGCC 9 cut(s) 5, 94, 134, 1220, 1497, 1958, 2048, 2725, 3157
BshNI GGYRCC 2 cut(s) 82, 2284
BshTI ACCGGT 2 cut(s) 565, 1892
BshVI ATCGAT 1 cut(s) 1752
BsiEI CGRYCG 1 cut(s) 135
BsiHKAI GWGCWC 4 cut(s) 240, 368, 377, 1629
BsiHKCI CYCGRG 1 cut(s) 217
BslFI GGGAC 1 cut(s) 1260
BslI CCNNNNNNNGG 8 cut(s) 327, 342, 572, 703, 1227, 1285, 1311, 2662
BsmAI GTCTC 7 cut(s) 41, 165, 612, 1360, 1589, 1697, 2457
BsmBI CGTCTC 1 cut(s) 165
BsmFI GGGAC 1 cut(s) 1260
BsmI GAATGC 2 cut(s) 1099, 2555
BsnI GGCC 9 cut(s) 5, 94, 134, 1220, 1497, 1958, 2048, 2725, 3157
Bso31I GGTCTC 4 cut(s) 41, 612, 1697, 2457
BsoBI CYCGRG 1 cut(s) 217
Bsp1286I GDGCHC 4 cut(s) 240, 368, 377, 1629
Bsp13I TCCGGA 2 cut(s) 664, 2095
Bsp1407I TGTACA 2 cut(s) 1885, 2524
Bsp143I GATC 9 cut(s) 70, 195, 931, 1123, 1606, 1749, 2231, 2955, 3215
BspANI GGCC 9 cut(s) 5, 94, 134, 1220, 1497, 1958, 2048, 2725, 3157
BspDI ATCGAT 1 cut(s) 1752
BspEI TCCGGA 2 cut(s) 664, 2095
BspHI TCATGA 2 cut(s) 2328, 2701
BspLI GGNNCC 9 cut(s) 84, 189, 350, 504, 791, 1496, 2077, 2286, 3057
BspMAI CTGCAG 2 cut(s) 2173, 2914
BspMI ACCTGC 1 cut(s) 234
BspPI GGATC 4 cut(s) 78, 203, 1118, 2226
BspT107I GGYRCC 2 cut(s) 82, 2284
BspTNI GGTCTC 4 cut(s) 41, 612, 1697, 2457
BsrDI GCAATG 1 cut(s) 352
BsrFI RCCGGY 4 cut(s) 100, 256, 565, 1892
BsrGI TGTACA 2 cut(s) 1885, 2524
BsrI ACTGG 3 cut(s) 842, 2453, 2976
BssAI RCCGGY 4 cut(s) 100, 256, 565, 1892
BssECI CCNNGG 8 cut(s) 6, 78, 89, 191, 508, 918, 1200, 1687
BssMI GATC 9 cut(s) 70, 195, 931, 1123, 1606, 1749, 2231, 2955, 3215
BssNAI GTATAC 1 cut(s) 1911
BssNI GRCGYC 4 cut(s) 83, 119, 158, 2586
BssSI CACGAG 1 cut(s) 2567
BssT1I CCWWGG 3 cut(s) 508, 918, 1200
Bst1107I GTATAC 1 cut(s) 1911
Bst2BI CACGAG 1 cut(s) 2567
Bst2UI CCWGG 6 cut(s) 193, 370, 966, 2073, 2825, 3060
Bst4CI ACNGT 7 cut(s) 35, 654, 898, 1101, 2185, 2548, 3011
Bst6I CTCTTC 3 cut(s) 1791, 2353, 2460
BstACI GRCGYC 4 cut(s) 83, 119, 158, 2586
BstAPI GCANNNNNTGC 1 cut(s) 2888
BstAUI TGTACA 2 cut(s) 1885, 2524
BstBAI YACGTR 1 cut(s) 235
BstC8I GCNNGC 4 cut(s) 258, 1252, 2319, 2730
BstDSI CCRYGG 1 cut(s) 78
BstEII GGTNACC 1 cut(s) 331
BstENI CCTNNNNNAGG 1 cut(s) 701
BstF5I GGATG 7 cut(s) 213, 393, 928, 1414, 1479, 2214, 3130
BstH2I RGCGCY 2 cut(s) 86, 263
BstHHI GCGC 4 cut(s) 85, 146, 262, 2794
BstKTI GATC 9 cut(s) 73, 198, 934, 1126, 1609, 1752, 2234, 2958, 3218
BstMAI GTCTC 7 cut(s) 41, 165, 612, 1360, 1589, 1697, 2457
BstMBI GATC 9 cut(s) 70, 195, 931, 1123, 1606, 1749, 2231, 2955, 3215
BstMCI CGRYCG 1 cut(s) 135
BstNI CCWGG 6 cut(s) 193, 370, 966, 2073, 2825, 3060
BstNSI RCATGY 1 cut(s) 2897
BstPAI GACNNNNGTC 1 cut(s) 33
BstPI GGTNACC 1 cut(s) 331
BstSCI CCNGG 9 cut(s) 191, 207, 335, 368, 682, 964, 2071, 2823, 3058
BstSFI CTRYAG 3 cut(s) 2169, 2505, 2910
BstSLI GKGCMC 1 cut(s) 240
BstV1I GCAGC 5 cut(s) 368, 1495, 1867, 2183, 2894
BstV2I GAAGAC 1 cut(s) 2549
BstX2I RGATCY 1 cut(s) 2231
BstXI CCANNNNNNTGG 4 cut(s) 199, 400, 890, 980
BstYI RGATCY 1 cut(s) 2231
BstZ17I GTATAC 1 cut(s) 1911
BstZI CGGCCG 1 cut(s) 132
Bsu15I ATCGAT 1 cut(s) 1752
BsuI GTATCC 2 cut(s) 1981, 2634
BsuRI GGCC 9 cut(s) 5, 94, 134, 1220, 1497, 1958, 2048, 2725, 3157
BsuTUI ATCGAT 1 cut(s) 1752
BtgI CCRYGG 1 cut(s) 78
BtgZI GCGATG 1 cut(s) 1392
BtsCI GGATG 7 cut(s) 213, 393, 928, 1414, 1479, 2214, 3130
BtsI GCAGTG 1 cut(s) 2994
BtsIMutI CAGTG 5 cut(s) 894, 1097, 2446, 2983, 2994
BveI ACCTGC 1 cut(s) 234
Cac8I GCNNGC 4 cut(s) 258, 1252, 2319, 2730
CciI TCATGA 2 cut(s) 2328, 2701
CciNI GCGGCCGC 1 cut(s) 132
CfoI GCGC 4 cut(s) 85, 146, 262, 2794
Cfr10I RCCGGY 4 cut(s) 100, 256, 565, 1892
Cfr13I GGNCC 5 cut(s) 187, 349, 1495, 3155, 3298
ClaI ATCGAT 1 cut(s) 1752
CseI GACGC 6 cut(s) 108, 346, 1262, 1382, 2017, 2575
Csp6I GTAC 9 cut(s) 172, 694, 785, 1763, 1886, 2285, 2525, 2642, 3015
CspAI ACCGGT 2 cut(s) 565, 1892
CspCI CAANNNNNGTGG 2 cut(s) 2004, 2039
CviQI GTAC 9 cut(s) 172, 694, 785, 1763, 1886, 2285, 2525, 2642, 3015
DinI GGCGCC 1 cut(s) 84
DpnI GATC 9 cut(s) 72, 197, 933, 1125, 1608, 1751, 2233, 2957, 3217
DpnII GATC 9 cut(s) 70, 195, 931, 1123, 1606, 1749, 2231, 2955, 3215
DraI TTTAAA 1 cut(s) 814
DriI GACNNNNNGTC 1 cut(s) 3235
EaeI YGGCCR 4 cut(s) 3, 132, 1218, 2046
EagI CGGCCG 1 cut(s) 132
Eam1104I CTCTTC 3 cut(s) 1791, 2353, 2460
Eam1105I GACNNNNNGTC 1 cut(s) 3235
EarI CTCTTC 3 cut(s) 1791, 2353, 2460
Ecl136II GAGCTC 2 cut(s) 366, 375
EclXI CGGCCG 1 cut(s) 132
Eco130I CCWWGG 3 cut(s) 508, 918, 1200
Eco24I GRGCYC 2 cut(s) 368, 377
Eco31I GGTCTC 4 cut(s) 41, 612, 1697, 2457
Eco32I GATATC 2 cut(s) 183, 1416
Eco47I GGWCC 3 cut(s) 187, 349, 3298
Eco52I CGGCCG 1 cut(s) 132
Eco53kI GAGCTC 2 cut(s) 366, 375
Eco57I CTGAAG 5 cut(s) 1125, 2112, 2232, 2452, 2561
Eco72I CACGTG 1 cut(s) 235
Eco88I CYCGRG 1 cut(s) 217
Eco91I GGTNACC 1 cut(s) 331
EcoICRI GAGCTC 2 cut(s) 366, 375
EcoNI CCTNNNNNAGG 1 cut(s) 701
EcoO109I RGGNCCY 1 cut(s) 3155
EcoO65I GGTNACC 1 cut(s) 331
EcoRII CCWGG 6 cut(s) 191, 368, 964, 2071, 2823, 3058
EcoRV GATATC 2 cut(s) 183, 1416
EcoT14I CCWWGG 3 cut(s) 508, 918, 1200
EcoT38I GRGCYC 2 cut(s) 368, 377
EgeI GGCGCC 1 cut(s) 84
EheI GGCGCC 1 cut(s) 84
ErhI CCWWGG 3 cut(s) 508, 918, 1200
Esp3I CGTCTC 1 cut(s) 165
FalI AAGNNNNNCTT 2 cut(s) 3258, 3290
FaqI GGGAC 1 cut(s) 1260
FauI CCCGC 1 cut(s) 261
FauNDI CATATG 1 cut(s) 865
FbaI TGATCA 1 cut(s) 931
FblI GTMKAC 3 cut(s) 27, 435, 1910
FokI GGATG 7 cut(s) 200, 380, 935, 1421, 1486, 2201, 3137
FriOI GRGCYC 2 cut(s) 368, 377
FspBI CTAG 4 cut(s) 422, 765, 1311, 2561
GlaI GCGC 4 cut(s) 84, 145, 261, 2793
GsaI CCCAGC 1 cut(s) 141
GsuI CTGGAG 4 cut(s) 314, 352, 2055, 3042
HaeII RGCGCY 2 cut(s) 86, 263
HaeIII GGCC 9 cut(s) 5, 94, 134, 1220, 1497, 1958, 2048, 2725, 3157
HgaI GACGC 6 cut(s) 108, 346, 1262, 1382, 2017, 2575
HhaI GCGC 4 cut(s) 85, 146, 262, 2794
Hin1I GRCGYC 4 cut(s) 83, 119, 158, 2586
Hin6I GCGC 4 cut(s) 83, 144, 260, 2792
HinP1I GCGC 4 cut(s) 83, 144, 260, 2792
HincII GTYRAC 5 cut(s) 28, 436, 835, 843, 3135
HindII GTYRAC 5 cut(s) 28, 436, 835, 843, 3135
HindIII AAGCTT 1 cut(s) 2090
HphI GGTGA 8 cut(s) 325, 541, 903, 1268, 2363, 2606, 2950, 3017
Hpy99I CGWCG 8 cut(s) 29, 32, 35, 124, 127, 130, 160, 2033
HpyCH4III ACNGT 7 cut(s) 35, 654, 898, 1101, 2185, 2548, 3011
HpyCH4IV ACGT 3 cut(s) 158, 234, 2043
HpySE526I ACGT 3 cut(s) 158, 234, 2043
Hsp92I GRCGYC 4 cut(s) 83, 119, 158, 2586
HspAI GCGC 4 cut(s) 83, 144, 260, 2792
KasI GGCGCC 1 cut(s) 82
Kpn2I TCCGGA 2 cut(s) 664, 2095
KpnI GGTACC 1 cut(s) 2288
KroI GCCGGC 1 cut(s) 256
KroNI GCCGGC 1 cut(s) 258
Ksp22I TGATCA 1 cut(s) 931
Kzo9I GATC 9 cut(s) 70, 195, 931, 1123, 1606, 1749, 2231, 2955, 3215
LmnI GCTCC 7 cut(s) 371, 372, 789, 1632, 2075, 2400, 3061
Lsp1109I GCAGC 5 cut(s) 368, 1495, 1867, 2183, 2894
LweI GCATC 7 cut(s) 1245, 1287, 1399, 1464, 1495, 1759, 2233
MaeI CTAG 4 cut(s) 422, 765, 1311, 2561
MaeII ACGT 3 cut(s) 158, 234, 2043
MalI GATC 9 cut(s) 72, 197, 933, 1125, 1608, 1751, 2233, 2957, 3217
MboI GATC 9 cut(s) 70, 195, 931, 1123, 1606, 1749, 2231, 2955, 3215
MfeI CAATTG 3 cut(s) 473, 1485, 2877
MflI RGATCY 1 cut(s) 2231
MhlI GDGCHC 4 cut(s) 240, 368, 377, 1629
Mly113I GGCGCC 1 cut(s) 83
MlyI GAGTC 5 cut(s) 588, 1669, 2297, 2692, 3265
MmeI TCCRAC 4 cut(s) 1329, 2553, 2976, 3219
MreI CGCCGGCG 1 cut(s) 256
MroI TCCGGA 2 cut(s) 664, 2095
MroNI GCCGGC 1 cut(s) 256
MroXI GAANNNNTTC 2 cut(s) 1444, 2709
MslI CAYNNNNRTG 4 cut(s) 398, 491, 1893, 2507
MspA1I CMGCKG 3 cut(s) 137, 1730, 2885
MspR9I CCNGG 9 cut(s) 193, 209, 337, 370, 684, 966, 2073, 2825, 3060
MunI CAATTG 3 cut(s) 473, 1485, 2877
Mva1269I GAATGC 2 cut(s) 1099, 2555
MvaI CCWGG 6 cut(s) 193, 370, 966, 2073, 2825, 3060
NaeI GCCGGC 1 cut(s) 258
NarI GGCGCC 1 cut(s) 83
NciI CCSGG 3 cut(s) 209, 337, 684
NdeI CATATG 1 cut(s) 865
NdeII GATC 9 cut(s) 70, 195, 931, 1123, 1606, 1749, 2231, 2955, 3215
NgoMIV GCCGGC 1 cut(s) 256
NlaIV GGNNCC 9 cut(s) 84, 189, 350, 504, 791, 1496, 2077, 2286, 3057
NmeAIII GCCGAG 2 cut(s) 31, 114
NmuCI GTSAC 9 cut(s) 331, 871, 909, 2695, 3005, 3070, 3172, 3184, 3268
NotI GCGGCCGC 1 cut(s) 132
NspI RCATGY 1 cut(s) 2897
OliI CACNNNNGTG 2 cut(s) 1893, 2507
PagI TCATGA 2 cut(s) 2328, 2701
PaqCI CACCTGC 1 cut(s) 234
PciI ACATGT 1 cut(s) 2893
PcsI WCGNNNNNNNCGW 2 cut(s) 122, 246
PctI GAATGC 2 cut(s) 1099, 2555
PdiI GCCGGC 1 cut(s) 258
PdmI GAANNNNTTC 2 cut(s) 1444, 2709
PfeI GAWTC 8 cut(s) 1133, 1214, 1231, 1573, 1683, 1722, 2380, 2536
PflMI CCANNNNNTGG 1 cut(s) 1227
PfoI TCCNGGA 4 cut(s) 368, 964, 2071, 3058
PinAI ACCGGT 2 cut(s) 565, 1892
PleI GAGTC 5 cut(s) 587, 1668, 2297, 2692, 3265
PluTI GGCGCC 1 cut(s) 86
PmaCI CACGTG 1 cut(s) 235
PmlI CACGTG 1 cut(s) 235
PpsI GAGTC 5 cut(s) 587, 1668, 2297, 2692, 3265
Ppu21I YACGTR 1 cut(s) 235
PscI ACATGT 1 cut(s) 2893
PshAI GACNNNNGTC 1 cut(s) 33
PsiI TTATAA 1 cut(s) 2691
Psp124BI GAGCTC 2 cut(s) 368, 377
Psp6I CCWGG 6 cut(s) 191, 368, 964, 2071, 2823, 3058
PspCI CACGTG 1 cut(s) 235
PspEI GGTNACC 1 cut(s) 331
PspFI CCCAGC 1 cut(s) 137
PspGI CCWGG 6 cut(s) 191, 368, 964, 2071, 2823, 3058
PspN4I GGNNCC 9 cut(s) 84, 189, 350, 504, 791, 1496, 2077, 2286, 3057
PspPI GGNCC 5 cut(s) 187, 349, 1495, 3155, 3298
PstI CTGCAG 2 cut(s) 2173, 2914
PsuI RGATCY 1 cut(s) 2231
PvuII CAGCTG 2 cut(s) 1730, 2885
RsaI GTAC 9 cut(s) 173, 695, 786, 1764, 1887, 2286, 2526, 2643, 3016
RsaNI GTAC 9 cut(s) 172, 694, 785, 1763, 1886, 2285, 2525, 2642, 3015
RseI CAYNNNNRTG 4 cut(s) 398, 491, 1893, 2507
SacI GAGCTC 2 cut(s) 368, 377
SalI GTCGAC 2 cut(s) 26, 434
Sau3AI GATC 9 cut(s) 70, 195, 931, 1123, 1606, 1749, 2231, 2955, 3215
Sau96I GGNCC 5 cut(s) 187, 349, 1495, 3155, 3298
ScaI AGTACT 1 cut(s) 2643
SchI GAGTC 5 cut(s) 588, 1669, 2297, 2692, 3265
ScrFI CCNGG 9 cut(s) 193, 209, 337, 370, 684, 966, 2073, 2825, 3060
SduI GDGCHC 4 cut(s) 240, 368, 377, 1629
SfaNI GCATC 7 cut(s) 1245, 1287, 1399, 1464, 1495, 1759, 2233
SfcI CTRYAG 3 cut(s) 2169, 2505, 2910
SfoI GGCGCC 1 cut(s) 84
SgrAI CRCCGGYG 2 cut(s) 256, 1892
SgrDI CGTCGACG 1 cut(s) 26
SinI GGWCC 3 cut(s) 187, 349, 3298
SmiMI CAYNNNNRTG 4 cut(s) 398, 491, 1893, 2507
SmlI CTYRAG 3 cut(s) 1592, 1859, 2375
SmoI CTYRAG 3 cut(s) 1592, 1859, 2375
SpeI ACTAGT 1 cut(s) 764
SspDI GGCGCC 1 cut(s) 82
SspI AATATT 2 cut(s) 1267, 1744
SspMI CTAG 4 cut(s) 422, 765, 1311, 2561
SstI GAGCTC 2 cut(s) 368, 377
StyD4I CCNGG 9 cut(s) 191, 207, 335, 368, 682, 964, 2071, 2823, 3058
StyI CCWWGG 3 cut(s) 508, 918, 1200
TaaI ACNGT 7 cut(s) 35, 654, 898, 1101, 2185, 2548, 3011
TaiI ACGT 3 cut(s) 161, 237, 2046
TaqI TCGA 9 cut(s) 27, 47, 73, 300, 435, 1212, 1537, 1752, 3051
TaqII GACCGA 1 cut(s) 2507
TatI WGTACW 5 cut(s) 1762, 1885, 2524, 2641, 3014
TauI GCSGC 9 cut(s) 88, 97, 100, 134, 137, 232, 2051, 2126, 2728
TfiI GAWTC 8 cut(s) 1133, 1214, 1231, 1573, 1683, 1722, 2380, 2536
TscAI CASTG 5 cut(s) 901, 1104, 2453, 2983, 2994
TseFI GTSAC 9 cut(s) 331, 871, 909, 2695, 3005, 3070, 3172, 3184, 3268
TseI GCWGC 5 cut(s) 381, 1508, 1855, 2171, 2882
Tsp45I GTSAC 9 cut(s) 331, 871, 909, 2695, 3005, 3070, 3172, 3184, 3268
TspGWI ACGGA 2 cut(s) 503, 802
TspRI CASTG 5 cut(s) 901, 1104, 2453, 2983, 2994
Van91I CCANNNNNTGG 1 cut(s) 1227
VneI GTGCAC 1 cut(s) 236
VpaK11BI GGWCC 3 cut(s) 187, 349, 3298
XagI CCTNNNNNAGG 1 cut(s) 701
XapI RAATTY 6 cut(s) 747, 2109, 2494, 2513, 2603, 2647
XceI RCATGY 1 cut(s) 2897
XmiI GTMKAC 3 cut(s) 27, 435, 1910
XmnI GAANNNNTTC 2 cut(s) 1444, 2709
XspI CTAG 4 cut(s) 422, 765, 1311, 2561
ZraI GACGTC 1 cut(s) 159
ZrmI AGTACT 1 cut(s) 2643
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.