Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0009166

Physical Location & Seq

Genomic Coordinates Forward (+)

21204465 .. 21214504

Gene Structure 10040 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1251700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1839 bp

ATGGAGAGGGTGAGAGCCAGTAATCGTCTCCTCAGATACTCCTCCAATAAGTCACTCCTTGATCGTCGATTGAATCCCAGTTCTCAGAATTCAATCCACAACTCCCCTTCTGGTTATTCTCATGGTTTTGTCAACAAGTGTAGAGATTTCCATCTCTACAATGGTAATCACAAGTGGAGTGTCCAGACATGTTTCGGATTCTTAAGATATCGGGGAGAAACTTTTCGGGATTCTGTCGAAAGGGGTTGTCTTGAAAAGGCTTTCCGGTGTCGAGGTTTTGGTTCATTGGCGGCGAAGGTTGAGAGGAACCCGTCTTTTTCGACGTTGAATGCAGATGATATCAGCTATTTTAAGGGAGTACTAGGGGAGAAGACTGTAGTTCAAGAGGAAGAGAGGCTGGCCATTGCAAATACAGACTGGATGCACAAATACAAAGGCTCGAGTAAGCTTCTGCTGCAACCGCAGAGCACTGAGGAGGTGTCACAAATTCTTAAATATTGTAACTCCAGATGCTTGGCTGTTGTTCCCCAAGGCGGAAATACTGGTCTTGTGGGCGGAAGTGTTCCAGTTTTCGATGAAGTGATTATCAATATGAGTTTGATGAATAACATTATATCTTTTGACCAGGTGAGTGGTATACTGGTATGCGAGGCAGGATGCATATTGGAAAATCTGATTACTTTTTTGGACAACCAAGGATTTATTATGCCACTGGACTTAGGTGCAAAAGGAAGCTGCCAGATTGGTGGAAATGTTTCAACCAATGCAGGTGGTTTGCGCCTTGTTCGCTATGGATCACTTCATGGAAACGTACTTGGTATAGAAGCTGTTTTGGCTGATGGTAATGTTATTAACATGCTTGGAACTTTACGCAAAGATAATACTGGGTATGACCTAAAGCATTTGTTCATAGGAAGTGAAGGATCTTTGGGTATTGTGACCAAAGTTTCTATACTTACCCCTCCAAAGTTATTCTCAGTAAATGTAGCTTTCCTTGCATGCCAAGATTACTTTAGCTGTCAAAATCTTCTAGTGGAAGCAAAGAGGAAGCTTGGGGAGATTCTATCGGCTTTTGAATTCTTGGATAGCCAGTCACTGGATTTGGTTCTGAATCACTTGGATGGCGTCCGGAATCCATTACCTCCCACAATGCACAACTTTTATATTTTGATTGAGACAACAGGCAGTGATGAAACTTATGACAGACAGAAGCTTGAAGCCTTCCTACTATATGCAATGGAAGCTGGTTTGGTTTCTGATGGTGCTCTAGCACAAGACATAAACCAAGCATCATCATTCTGGCGTATTCGTGAGGGAGTACCAGAAGCTTTGATGAAAGCAGGAGCTGTTTACAAATATGATCTATCATTACCTGTTGAAAAGATGTATGATCTTGTCGAGGAAATGAGAAAAAGACTTAGTGATTCAGCTAACGTAGTTGGATATGGCCACCTTGGAGATAGTAATTTGCATCTCAATATTTCAGCTCCACAGTACGATGATAGGATTTTGACACAAATTGAACCATTTGTCTACGAGTGGACGTCTAAGCACCGTGGGAGCATTAGTGCAGAGCATGGCCTTGGACTGATGAAAGCTAATGAGATTTTCTACAGCAAGCCAACTGCTACGGTGACACTGATGGCTTCCATCAAGAAAATGCTGGACCCGAAAGGGATACTCAACCCATACAAAGTTCTTCCGCACTCCTTTAATGAAACCGAGGCTTTCATCATCTTTACCCTCGAGTTGTCTCTTGTCCATTGCAACTTTACACAGCTAACTCATGTTTATGTCAACGGTTCAGTCGCTCTGGCACCCTCGAACTCCTGCTGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0004457 GO:0004458 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006082 GO:0006089 GO:0006091 GO:0006807 GO:0008150 GO:0008152 GO:0009853 GO:0009987 GO:0015980 GO:0016491 GO:0016614 GO:0016898 GO:0019516 GO:0019752 GO:0022900 GO:0022904 GO:0032787 GO:0036094 GO:0043094 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0045333 GO:0047545 GO:0048037 GO:0050660 GO:0050662 GO:0051990 GO:0055114 GO:0071704 GO:0071949 GO:0097159 GO:1901265 GO:1901363 GO:1901615

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FAD-oxidase_C FAD_binding_4

Protein Analysis

613

Amino Acids

67.79

Weight (kDa)

6.36

Isoelectric Point (pI)

41.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FAD_binding_4	PF01565	150 - 285	2.1e-34	FAD binding domain
FAD-oxidase_C	PF02913	327 - 566	1e-59	FAD linked oxidases, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009166)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G36400 AT4G36400
fragaria_vesca	FvH4_1g12310 FvH4_1g12310 FvH4_1g12310
malus_domestica	MD15G1251700.v1.1
prunus_persica	Prupe.7G162700_v2.0.a1 Prupe.7G162700_v2.0.a1 Prupe.7G162700_v2.0.a1 Prupe.7G162700_v2.0.a1 Prupe.7G162700_v2.0.a1 Prupe.7G162700_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0100131
rosa_laevigata	RLG00000016939
rosa_multiflora	Rmu_sc0018554.1_g000002
rosa_roxburghii	Rroxscaffold_2G00142540
rosa_rugosa	Rorug02G0085900
rosa_samantha	Rh2AG134600 Rh2BG138300 Rh2CG140100 Rh2DG140300
rosa_wichuraiana	Rw2G010520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	758
AatII	GACGTC	1 cut(s)	1547
Acc36I	ACCTGC	1 cut(s)	758
AccB1I	GGYRCC	1 cut(s)	1816
AccB7I	CCANNNNNTGG	1 cut(s)	1096
AccI	GTMKAC	2 cut(s)	637, 1533
AccIII	TCCGGA	1 cut(s)	1128
AciI	CCGC	5 cut(s)	290, 461, 534, 555, 1703
AclWI	GGATC	2 cut(s)	802, 931
AcoI	YGGCCR	2 cut(s)	399, 1447
AcsI	RAATTY	3 cut(s)	88, 486, 1076
AcyI	GRCGYC	2 cut(s)	1125, 1544
AfaI	GTAC	4 cut(s)	360, 813, 1320, 1496
AfiI	CCNNNNNNNGG	2 cut(s)	533, 1096
AflII	CTTAAG	1 cut(s)	202
AflIII	ACRYGT	1 cut(s)	188
AjnI	CCWGG	1 cut(s)	624
Alw21I	GWGCWC	2 cut(s)	470, 1267
Alw26I	GTCTC	3 cut(s)	32, 1169, 1758
AlwI	GGATC	2 cut(s)	802, 931
AlwNI	CAGNNNCTG	2 cut(s)	1096, 1346
Ama87I	CYCGRG	2 cut(s)	439, 1745
Aor13HI	TCCGGA	1 cut(s)	1128
AoxI	GGCC	3 cut(s)	399, 1447, 1579
ApeKI	GCWGC	3 cut(s)	454, 735, 1833
ApoI	RAATTY	3 cut(s)	88, 486, 1076
Asp700I	GAANNNNTTC	2 cut(s)	222, 754
AspLEI	GCGC	1 cut(s)	780
AspS9I	GGNCC	1 cut(s)	1666
AsuHPI	GGTGA	3 cut(s)	22, 640, 1645
AvaI	CYCGRG	2 cut(s)	439, 1745
AvaII	GGWCC	1 cut(s)	1666
BalI	TGGCCA	2 cut(s)	401, 1449
BanI	GGYRCC	1 cut(s)	1816
BarI	GAAGNNNNNNTAC	2 cut(s)	1209, 1241
BbsI	GAAGAC	1 cut(s)	377
Bbv12I	GWGCWC	2 cut(s)	470, 1267
BbvI	GCAGC	3 cut(s)	441, 722, 1820
BccI	CCATC	6 cut(s)	159, 833, 1115, 1253, 1636, 1658
BciT130I	CCWGG	1 cut(s)	626
BciVI	GTATCC	1 cut(s)	1671
BcoDI	GTCTC	3 cut(s)	32, 1169, 1758
BfaI	CTAG	3 cut(s)	362, 1031, 1268
BfmI	CTRYAG	2 cut(s)	375, 1612
BfrI	CTTAAG	1 cut(s)	202
BfuAI	ACCTGC	1 cut(s)	758
BfuI	GTATCC	1 cut(s)	1671
BisI	GCNGC	4 cut(s)	291, 455, 736, 1834
BlsI	GCNGC	4 cut(s)	292, 456, 737, 1835
BmcAI	AGTACT	1 cut(s)	360
Bme1390I	CCNGG	1 cut(s)	626
Bme18I	GGWCC	1 cut(s)	1666
BmeT110I	CYCGRG	2 cut(s)	439, 1745
BmgT120I	GGNCC	1 cut(s)	1666
BmiI	GGNNCC	3 cut(s)	308, 1668, 1818
BmrFI	CCNGG	1 cut(s)	626
BmrI	ACTGGG	2 cut(s)	72, 894
BmsI	GCATC	5 cut(s)	411, 500, 647, 1298, 1480
BmuI	ACTGGG	2 cut(s)	72, 894
BpiI	GAAGAC	1 cut(s)	377
BpmI	CTGGAG	1 cut(s)	490
BsaBI	GATNNNNATC	1 cut(s)	150
BsaHI	GRCGYC	2 cut(s)	1125, 1544
BsaJI	CCNNGG	6 cut(s)	529, 694, 1453, 1555, 1582, 1722
BsaWI	WCCGGW	2 cut(s)	264, 1128
BsaXI	ACNNNNNCTCC	2 cut(s)	1552, 1582
Bsc4I	CCNNNNNNNGG	2 cut(s)	533, 1096
Bse3DI	GCAATG	3 cut(s)	402, 1242, 1762
Bse8I	GATNNNNATC	1 cut(s)	150
BseAI	TCCGGA	1 cut(s)	1128
BseBI	CCWGG	1 cut(s)	626
BseDI	CCNNGG	6 cut(s)	529, 694, 1453, 1555, 1582, 1722
BseGI	GGATG	3 cut(s)	426, 662, 1126
BseJI	GATNNNNATC	1 cut(s)	150
BseLI	CCNNNNNNNGG	2 cut(s)	533, 1096
BseMI	GCAATG	3 cut(s)	402, 1242, 1762
BseMII	CTCAG	4 cut(s)	46, 98, 462, 990
BseRI	GAGGAG	3 cut(s)	20, 31, 488
BseXI	GCAGC	3 cut(s)	441, 722, 1820
BsgI	GTGCAG	1 cut(s)	1590
BshFI	GGCC	3 cut(s)	401, 1449, 1581
BshNI	GGYRCC	1 cut(s)	1816
BsiHKAI	GWGCWC	2 cut(s)	470, 1267
BsiHKCI	CYCGRG	2 cut(s)	439, 1745
BsiSI	CCGG	2 cut(s)	265, 1129
BslI	CCNNNNNNNGG	2 cut(s)	533, 1096
BsmAI	GTCTC	3 cut(s)	32, 1169, 1758
BsmBI	CGTCTC	1 cut(s)	32
BsmI	GAATGC	1 cut(s)	334
BsnI	GGCC	3 cut(s)	401, 1449, 1581
BsoBI	CYCGRG	2 cut(s)	439, 1745
Bsp1286I	GDGCHC	2 cut(s)	470, 1267
Bsp13I	TCCGGA	1 cut(s)	1128
Bsp143I	GATC	5 cut(s)	61, 794, 923, 1360, 1390
BspACI	CCGC	5 cut(s)	290, 461, 534, 555, 1703
BspANI	GGCC	3 cut(s)	401, 1449, 1581
BspCNI	CTCAG	4 cut(s)	45, 97, 463, 989
BspEI	TCCGGA	1 cut(s)	1128
BspLI	GGNNCC	3 cut(s)	308, 1668, 1818
BspMI	ACCTGC	1 cut(s)	758
BspPI	GGATC	2 cut(s)	802, 931
BspT107I	GGYRCC	1 cut(s)	1816
BspTI	CTTAAG	1 cut(s)	202
BsrDI	GCAATG	3 cut(s)	402, 1242, 1762
BssECI	CCNNGG	6 cut(s)	529, 694, 1453, 1555, 1582, 1722
BssMI	GATC	5 cut(s)	61, 794, 923, 1360, 1390
BssNAI	GTATAC	1 cut(s)	638
BssNI	GRCGYC	2 cut(s)	1125, 1544
BssT1I	CCWWGG	4 cut(s)	529, 694, 1453, 1582
Bst1107I	GTATAC	1 cut(s)	638
Bst2UI	CCWGG	1 cut(s)	626
Bst4CI	ACNGT	5 cut(s)	376, 1494, 1556, 1633, 1802
Bst6I	CTCTTC	1 cut(s)	384
BstACI	GRCGYC	2 cut(s)	1125, 1544
BstAFI	CTTAAG	1 cut(s)	202
BstC8I	GCNNGC	3 cut(s)	399, 1000, 1619
BstDEI	CTNAG	7 cut(s)	32, 84, 471, 718, 976, 1418, 1548
BstDSI	CCRYGG	1 cut(s)	1555
BstF5I	GGATG	3 cut(s)	426, 662, 1126
BstHHI	GCGC	1 cut(s)	780
BstKTI	GATC	5 cut(s)	64, 797, 926, 1363, 1393
BstMAI	GTCTC	3 cut(s)	32, 1169, 1758
BstMBI	GATC	5 cut(s)	61, 794, 923, 1360, 1390
BstMWI	GCNNNNNNNGC	6 cut(s)	454, 460, 786, 833, 995, 1241
BstNI	CCWGG	1 cut(s)	626
BstNSI	RCATGY	3 cut(s)	192, 859, 1002
BstSCI	CCNGG	1 cut(s)	624
BstSFI	CTRYAG	2 cut(s)	375, 1612
BstV1I	GCAGC	3 cut(s)	441, 722, 1820
BstV2I	GAAGAC	1 cut(s)	377
BstX2I	RGATCY	1 cut(s)	923
BstXI	CCANNNNNNTGG	3 cut(s)	514, 632, 746
BstYI	RGATCY	1 cut(s)	923
BstZ17I	GTATAC	1 cut(s)	638
BsuI	GTATCC	1 cut(s)	1671
BsuRI	GGCC	3 cut(s)	401, 1449, 1581
BtgI	CCRYGG	1 cut(s)	1555
BtsCI	GGATG	3 cut(s)	426, 662, 1126
BtsI	GCAGTG	1 cut(s)	1192
BtsIMutI	CAGTG	5 cut(s)	468, 710, 1094, 1192, 1637
BveI	ACCTGC	1 cut(s)	758
Cac8I	GCNNGC	3 cut(s)	399, 1000, 1619
CaiI	CAGNNNCTG	2 cut(s)	1096, 1346
CfoI	GCGC	1 cut(s)	780
Cfr13I	GGNCC	1 cut(s)	1666
CseI	GACGC	1 cut(s)	1114
CsiI	ACCWGGT	1 cut(s)	624
Csp6I	GTAC	4 cut(s)	359, 812, 1319, 1495
CspCI	CAANNNNNGTGG	2 cut(s)	751, 786
CviAII	CATG	7 cut(s)	122, 189, 803, 856, 999, 1577, 1787
CviQI	GTAC	4 cut(s)	359, 812, 1319, 1495
DdeI	CTNAG	7 cut(s)	32, 84, 471, 718, 976, 1418, 1548
DpnI	GATC	5 cut(s)	63, 796, 925, 1362, 1392
DpnII	GATC	5 cut(s)	61, 794, 923, 1360, 1390
EaeI	YGGCCR	2 cut(s)	399, 1447
Eam1104I	CTCTTC	1 cut(s)	384
EarI	CTCTTC	1 cut(s)	384
EciI	GGCGGA	2 cut(s)	549, 570
Eco130I	CCWWGG	4 cut(s)	529, 694, 1453, 1582
Eco32I	GATATC	2 cut(s)	209, 340
Eco47I	GGWCC	1 cut(s)	1666
Eco88I	CYCGRG	2 cut(s)	439, 1745
EcoRI	GAATTC	2 cut(s)	88, 1076
EcoRII	CCWGG	1 cut(s)	624
EcoRV	GATATC	2 cut(s)	209, 340
EcoT14I	CCWWGG	4 cut(s)	529, 694, 1453, 1582
EcoT22I	ATGCAT	1 cut(s)	662
ErhI	CCWWGG	4 cut(s)	529, 694, 1453, 1582
Esp3I	CGTCTC	1 cut(s)	32
FaeI	CATG	7 cut(s)	125, 192, 806, 859, 1002, 1580, 1790
FatI	CATG	7 cut(s)	121, 188, 802, 855, 998, 1576, 1786
FblI	GTMKAC	2 cut(s)	637, 1533
Fnu4HI	GCNGC	4 cut(s)	291, 455, 736, 1834
FokI	GGATG	3 cut(s)	433, 669, 1133
Fsp4HI	GCNGC	4 cut(s)	291, 455, 736, 1834
FspBI	CTAG	3 cut(s)	362, 1031, 1268
GlaI	GCGC	1 cut(s)	779
GluI	GCNGC	4 cut(s)	291, 455, 736, 1834
GsuI	CTGGAG	1 cut(s)	490
HaeIII	GGCC	3 cut(s)	401, 1449, 1581
HapII	CCGG	2 cut(s)	265, 1129
HgaI	GACGC	1 cut(s)	1114
HhaI	GCGC	1 cut(s)	780
Hin1I	GRCGYC	2 cut(s)	1125, 1544
Hin1II	CATG	7 cut(s)	125, 192, 806, 859, 1002, 1580, 1790
Hin6I	GCGC	1 cut(s)	778
HinP1I	GCGC	1 cut(s)	778
HincII	GTYRAC	2 cut(s)	133, 1798
HindII	GTYRAC	2 cut(s)	133, 1798
HindIII	AAGCTT	4 cut(s)	446, 1049, 1211, 1326
HinfI	GANTC	7 cut(s)	73, 198, 230, 1060, 1111, 1132, 1424
HpaII	CCGG	2 cut(s)	265, 1129
HphI	GGTGA	3 cut(s)	22, 640, 1645
Hpy166II	GTNNAC	6 cut(s)	133, 638, 1351, 1534, 1542, 1798
Hpy188I	TCNGA	6 cut(s)	35, 87, 197, 675, 1110, 1258
Hpy188III	TCNNGA	8 cut(s)	184, 227, 251, 383, 507, 1129, 1310, 1654
Hpy8I	GTNNAC	6 cut(s)	133, 638, 1351, 1534, 1542, 1798
Hpy99I	CGWCG	2 cut(s)	69, 325
HpyAV	CCTTC	4 cut(s)	117, 289, 914, 1231
HpyCH4III	ACNGT	5 cut(s)	376, 1494, 1556, 1633, 1802
HpyCH4IV	ACGT	4 cut(s)	323, 810, 1434, 1544
HpyF10VI	GCNNNNNNNGC	6 cut(s)	454, 460, 786, 833, 995, 1241
HpyF3I	CTNAG	7 cut(s)	32, 84, 471, 718, 976, 1418, 1548
HpySE526I	ACGT	4 cut(s)	323, 810, 1434, 1544
Hsp92I	GRCGYC	2 cut(s)	1125, 1544
Hsp92II	CATG	7 cut(s)	125, 192, 806, 859, 1002, 1580, 1790
HspAI	GCGC	1 cut(s)	778
Kpn2I	TCCGGA	1 cut(s)	1128
Kzo9I	GATC	5 cut(s)	61, 794, 923, 1360, 1390
LmnI	GCTCC	3 cut(s)	1343, 1492, 1560
Lsp1109I	GCAGC	3 cut(s)	441, 722, 1820
LweI	GCATC	5 cut(s)	411, 500, 647, 1298, 1480
MabI	ACCWGGT	1 cut(s)	624
MaeI	CTAG	3 cut(s)	362, 1031, 1268
MaeII	ACGT	4 cut(s)	323, 810, 1434, 1544
MaeIII	GTNAC	6 cut(s)	51, 480, 500, 937, 1092, 1633
MalI	GATC	5 cut(s)	63, 796, 925, 1362, 1392
MboI	GATC	5 cut(s)	61, 794, 923, 1360, 1390
MboII	GAAGA	4 cut(s)	382, 401, 1019, 1691
MflI	RGATCY	1 cut(s)	923
MhlI	GDGCHC	2 cut(s)	470, 1267
MlsI	TGGCCA	2 cut(s)	401, 1449
MluCI	AATT	5 cut(s)	88, 486, 1076, 1465, 1518
MluNI	TGGCCA	2 cut(s)	401, 1449
MmeI	TCCRAC	1 cut(s)	1420
Mox20I	TGGCCA	2 cut(s)	401, 1449
Mph1103I	ATGCAT	1 cut(s)	662
MroI	TCCGGA	1 cut(s)	1128
MroXI	GAANNNNTTC	2 cut(s)	222, 754
MscI	TGGCCA	2 cut(s)	401, 1449
MseI	TTAA	5 cut(s)	203, 351, 492, 852, 1713
MslI	CAYNNNNRTG	2 cut(s)	1119, 1791
Msp20I	TGGCCA	2 cut(s)	401, 1449
MspCI	CTTAAG	1 cut(s)	202
MspI	CCGG	2 cut(s)	265, 1129
MspR9I	CCNGG	1 cut(s)	626
Mva1269I	GAATGC	1 cut(s)	334
MvaI	CCWGG	1 cut(s)	626
MwoI	GCNNNNNNNGC	6 cut(s)	454, 460, 786, 833, 995, 1241
NdeII	GATC	5 cut(s)	61, 794, 923, 1360, 1390
NlaIII	CATG	7 cut(s)	125, 192, 806, 859, 1002, 1580, 1790
NlaIV	GGNNCC	3 cut(s)	308, 1668, 1818
NmuCI	GTSAC	5 cut(s)	51, 480, 937, 1092, 1633
NsiI	ATGCAT	1 cut(s)	662
NspI	RCATGY	3 cut(s)	192, 859, 1002
PaeI	GCATGC	1 cut(s)	1002
PaeR7I	CTCGAG	2 cut(s)	439, 1745
PaqCI	CACCTGC	1 cut(s)	758
PciI	ACATGT	1 cut(s)	188
PctI	GAATGC	1 cut(s)	334
PdmI	GAANNNNTTC	2 cut(s)	222, 754
PfeI	GAWTC	7 cut(s)	73, 198, 230, 1060, 1111, 1132, 1424
PflMI	CCANNNNNTGG	1 cut(s)	1096
PkrI	GCNGC	4 cut(s)	292, 456, 737, 1835
PscI	ACATGT	1 cut(s)	188
Psp6I	CCWGG	1 cut(s)	624
PspGI	CCWGG	1 cut(s)	624
PspN4I	GGNNCC	3 cut(s)	308, 1668, 1818
PspPI	GGNCC	1 cut(s)	1666
PspXI	VCTCGAGB	2 cut(s)	439, 1745
PstNI	CAGNNNCTG	2 cut(s)	1096, 1346
PsuI	RGATCY	1 cut(s)	923
RsaI	GTAC	4 cut(s)	360, 813, 1320, 1496
RsaNI	GTAC	4 cut(s)	359, 812, 1319, 1495
RseI	CAYNNNNRTG	2 cut(s)	1119, 1791
SaqAI	TTAA	5 cut(s)	203, 351, 492, 852, 1713
SatI	GCNGC	4 cut(s)	291, 455, 736, 1834
Sau3AI	GATC	5 cut(s)	61, 794, 923, 1360, 1390
Sau96I	GGNCC	1 cut(s)	1666
ScaI	AGTACT	1 cut(s)	360
ScrFI	CCNGG	1 cut(s)	626
SduI	GDGCHC	2 cut(s)	470, 1267
SexAI	ACCWGGT	1 cut(s)	624
SfaNI	GCATC	5 cut(s)	411, 500, 647, 1298, 1480
SfcI	CTRYAG	2 cut(s)	375, 1612
Sfr274I	CTCGAG	2 cut(s)	439, 1745
SinI	GGWCC	1 cut(s)	1666
SlaI	CTCGAG	2 cut(s)	439, 1745
SmiMI	CAYNNNNRTG	2 cut(s)	1119, 1791
SmlI	CTYRAG	3 cut(s)	202, 439, 1745
SmoI	CTYRAG	3 cut(s)	202, 439, 1745
SphI	GCATGC	1 cut(s)	1002
Sse9I	AATT	5 cut(s)	88, 486, 1076, 1465, 1518
SsiI	CCGC	5 cut(s)	290, 461, 534, 555, 1703
SspI	AATATT	2 cut(s)	497, 1480
SspMI	CTAG	3 cut(s)	362, 1031, 1268
StyD4I	CCNGG	1 cut(s)	624
StyI	CCWWGG	4 cut(s)	529, 694, 1453, 1582
TaaI	ACNGT	5 cut(s)	376, 1494, 1556, 1633, 1802
TaiI	ACGT	4 cut(s)	326, 813, 1437, 1547
TaqI	TCGA	9 cut(s)	67, 237, 271, 320, 440, 573, 1398, 1746, 1823
TasI	AATT	5 cut(s)	88, 486, 1076, 1465, 1518
TatI	WGTACW	1 cut(s)	358
TauI	GCSGC	1 cut(s)	293
TfiI	GAWTC	7 cut(s)	73, 198, 230, 1060, 1111, 1132, 1424
Tru1I	TTAA	5 cut(s)	203, 351, 492, 852, 1713
Tru9I	TTAA	5 cut(s)	203, 351, 492, 852, 1713
TscAI	CASTG	5 cut(s)	475, 717, 1101, 1192, 1644
TseFI	GTSAC	5 cut(s)	51, 480, 937, 1092, 1633
TseI	GCWGC	3 cut(s)	454, 735, 1833
Tsp45I	GTSAC	5 cut(s)	51, 480, 937, 1092, 1633
TspRI	CASTG	5 cut(s)	475, 717, 1101, 1192, 1644
Van91I	CCANNNNNTGG	1 cut(s)	1096
Vha464I	CTTAAG	1 cut(s)	202
VpaK11BI	GGWCC	1 cut(s)	1666
XapI	RAATTY	3 cut(s)	88, 486, 1076
XceI	RCATGY	3 cut(s)	192, 859, 1002
XcmI	CCANNNNNNNNNTGG	1 cut(s)	158
XhoI	CTCGAG	2 cut(s)	439, 1745
XmiI	GTMKAC	2 cut(s)	637, 1533
XmnI	GAANNNNTTC	2 cut(s)	222, 754
XspI	CTAG	3 cut(s)	362, 1031, 1268
ZraI	GACGTC	1 cut(s)	1545
ZrmI	AGTACT	1 cut(s)	360
Zsp2I	ATGCAT	1 cut(s)	662

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.