Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0009166

Physical Location & Seq

Genomic Coordinates Forward (+)

80604797 .. 80610146

Gene Structure 5350 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00142540.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1728 bp

ATGAGAATGGAGAAGCTGAGAGCCACCGGTCGTTTTCTCAGATACTCACCTAAAGTTTCAGTCTTTGATCGGCGATTGAGCTCTAATTCCGGCAAGTCAATCCACAGCTCCGCTTCAGGTTATGGGAATGGATTTGGCAAGAAATGTAGAGATGATGTTATCCGCAAGTGGAATGTTAGGACATGTTTTGGCTTGTATGGATATCAGGGGAAAAATTTCAGGGATTGTTTGGAAAGGGGTGTTCTTGAGGATACAAGGAGAATCCAGCATCGAGGTTTTGGTTCGCTGACCGAGAAGGTGCAGAGGAATGCTTCTTTTTCGACGTTGAATTCTGATGATATTAGCTATTTCAAGGAGATATTGGGTGAGAAGAATGTAGTTGAGGATGAACAGAGGCTGGCTATTGCTAATACAGATTGGATGCACAAGTACAGAGGCTCGAGTAAGCTTCTGCTGCAACCCCGGTCCACTGAGGAGGTTTCAGAGATTCTTAAATACTGTAATTCCAGAAACTTGGCTGTTGTTCCTCAAGGTGGAAATACTGGTCTTGTGGGCGGAAGTGTTCCTGTTTTTGATGAAGTGGTTGTCAATATGAGTTTGATGAATAATGTAATATCTTTTGACAAGGTGAGTGGTATACTCGTATGCGAGGCAGGATGCATATTGGAAAATCTGATTACTTTCTTGGATAACCAAGGATTTATTATGCCACTGGACTTAGGTGCGAAAGGAAGCTGCCAGATTGGTGGAAATGTTTCAACCAATGCGGGTGGTTTGCGCCTTGTGCGCTATGGATCACTTCATGGAACCATACTTGGTCTGGAAGTTGTTTTGGCTAATGGTGATGTTCTTGACATGCTTGGGACTTTACGGAAAGATAATACTGGATATGACCTAAAGCATTTGTTCATAGGAAGTGAAGGATCTTTGGGAATTGTGACCAAGGTTTCTATACTTACCCCTCCAAAGTTATTTTCAGTGAATGTAGCTTTCCTCGCATGCCAAGACTACTTTAGCTGCCAGAAACTTCTAGTGGAAGCAAAGAGAAAACTTGGGGAGATTCTATCTGCTTTTGAATTCTTGGATAGCCATGCTCTGGATTTGGTTCTGAATCATTTGGATGGCGTTCGCAATCCATTACCTCCCACAATTCACAACTTTTATGTTTTGATTGAGACAACGGGCAGTGATGAAACTTATGACAGAGAGAAGCTTGAAGCCTTCCTAGTTCATGCCATGGAAGGTGGTTTGATTTCTGATGGAGCTATAGCACAAGACATAAACCAAGCAGCAGCATTCTGGCATATACGCGAGGGAGTCCCAGAAGCATTGATGAAAGCTGGTGCTGTTTACAAATATGATTTATCATTACCTGTTGAAAAGATGTACGATCTTGTAGATGAAATGAGAACACGACTTGGTGATTCAGCTAATGTAGTTGGATATGGCCATCTTGGAGATGGCAATTTGCATCTTAATGTTTCAACTCCACAGTATGATGATAAGACTTTGGCACAAATTGAACCCTTTGTCTACGAGTGGACATCTGCGCATCGTGGAAGCATTAGTGCAGAGCATGGCCTTGGACTGATGAAAGCTAATAAGATTTTCTACAGCAAGCCAACTGCTACTGTGAACCTGATGGCCTCCATCAAGAAGATGCTGGACCCAAAAGGAATACTCAACCCCTATAAAGTTCTTCCGCACTCAATTGATTCTCACAGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0004457 GO:0004458 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006082 GO:0006089 GO:0006091 GO:0006807 GO:0008150 GO:0008152 GO:0009853 GO:0009987 GO:0015980 GO:0016491 GO:0016614 GO:0016898 GO:0019516 GO:0019752 GO:0022900 GO:0022904 GO:0032787 GO:0036094 GO:0043094 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0045333 GO:0047545 GO:0048037 GO:0050660 GO:0050662 GO:0051990 GO:0055114 GO:0071704 GO:0071949 GO:0097159 GO:1901265 GO:1901363 GO:1901615

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FAD-oxidase_C FAD_binding_4

Protein Analysis

575

Amino Acids

63.48

Weight (kDa)

6.77

Isoelectric Point (pI)

33.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FAD_binding_4	PF01565	150 - 285	1.9e-36	FAD binding domain
FAD-oxidase_C	PF02913	327 - 566	1.6e-60	FAD linked oxidases, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009166)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G36400 AT4G36400
fragaria_vesca	FvH4_1g12310 FvH4_1g12310 FvH4_1g12310
malus_domestica	MD15G1251700.v1.1
prunus_persica	Prupe.7G162700_v2.0.a1 Prupe.7G162700_v2.0.a1 Prupe.7G162700_v2.0.a1 Prupe.7G162700_v2.0.a1 Prupe.7G162700_v2.0.a1 Prupe.7G162700_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0100131
rosa_laevigata	RLG00000016939
rosa_multiflora	Rmu_sc0018554.1_g000002
rosa_roxburghii	Rroxscaffold_2G00142540
rosa_rugosa	Rorug02G0085900
rosa_samantha	Rh2AG134600 Rh2BG138300 Rh2CG140100 Rh2DG140300
rosa_wichuraiana	Rw2G010520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1551
AccB7I	CCANNNNNTGG	1 cut(s)	1096
AccI	GTMKAC	2 cut(s)	637, 1533
AccII	CGCG	1 cut(s)	1311
AciI	CCGC	5 cut(s)	111, 163, 555, 767, 1703
AclWI	GGATC	2 cut(s)	802, 931
AcoI	YGGCCR	1 cut(s)	1447
AcsI	RAATTY	3 cut(s)	214, 328, 1076
AcuI	CTGAAG	1 cut(s)	99
AfaI	GTAC	2 cut(s)	431, 1388
AfiI	CCNNNNNNNGG	2 cut(s)	533, 1096
AflIII	ACRYGT	1 cut(s)	182
AgeI	ACCGGT	1 cut(s)	26
AgsI	TTSAA	8 cut(s)	328, 352, 759, 1076, 1217, 1379, 1485, 1523
AjuI	GAANNNNNNNTTGG	2 cut(s)	958, 990
Alw21I	GWGCWC	1 cut(s)	83
Alw26I	GTCTC	1 cut(s)	1169
AlwI	GGATC	2 cut(s)	802, 931
AlwNI	CAGNNNCTG	1 cut(s)	397
Ama87I	CYCGRG	1 cut(s)	439
AoxI	GGCC	3 cut(s)	1447, 1579, 1644
ApeKI	GCWGC	5 cut(s)	454, 735, 1017, 1289, 1292
ApoI	RAATTY	3 cut(s)	214, 328, 1076
AsiGI	ACCGGT	1 cut(s)	26
Asp700I	GAANNNNTTC	2 cut(s)	215, 754
AspLEI	GCGC	3 cut(s)	780, 789, 1552
AspS9I	GGNCC	2 cut(s)	465, 1666
AsuC2I	CCSGG	1 cut(s)	463
AsuHPI	GGTGA	5 cut(s)	39, 377, 640, 854, 1433
AvaI	CYCGRG	1 cut(s)	439
AvaII	GGWCC	2 cut(s)	465, 1666
BalI	TGGCCA	1 cut(s)	1449
BanII	GRGCYC	1 cut(s)	83
Bbv12I	GWGCWC	1 cut(s)	83
BbvI	GCAGC	5 cut(s)	441, 722, 1004, 1301, 1304
BccI	CCATC	6 cut(s)	1115, 1253, 1454, 1458, 1636, 1658
BciVI	GTATCC	1 cut(s)	244
BcnI	CCSGG	1 cut(s)	463
BcoDI	GTCTC	1 cut(s)	1169
BfaI	CTAG	2 cut(s)	1031, 1226
BfmI	CTRYAG	2 cut(s)	1266, 1612
BfuI	GTATCC	1 cut(s)	244
BisI	GCNGC	5 cut(s)	455, 736, 1018, 1290, 1293
BlsI	GCNGC	5 cut(s)	456, 737, 1019, 1291, 1294
Bme1390I	CCNGG	1 cut(s)	463
Bme18I	GGWCC	2 cut(s)	465, 1666
BmeT110I	CYCGRG	1 cut(s)	439
BmgT120I	GGNCC	2 cut(s)	465, 1666
BmiI	GGNNCC	2 cut(s)	808, 1668
BmrFI	CCNGG	1 cut(s)	463
BmsI	GCATC	6 cut(s)	277, 411, 647, 1480, 1561, 1650
BpuEI	CTTGAG	2 cut(s)	266, 513
BpuMI	CCSGG	1 cut(s)	463
BsaJI	CCNNGG	5 cut(s)	461, 694, 942, 1236, 1582
BsaWI	WCCGGW	1 cut(s)	26
Bsc4I	CCNNNNNNNGG	2 cut(s)	533, 1096
Bse118I	RCCGGY	1 cut(s)	26
Bse1I	ACTGG	3 cut(s)	547, 717, 889
BseDI	CCNNGG	5 cut(s)	461, 694, 942, 1236, 1582
BseGI	GGATG	4 cut(s)	391, 426, 662, 1126
BseLI	CCNNNNNNNGG	2 cut(s)	533, 1096
BseMII	CTCAG	3 cut(s)	8, 52, 462
BseNI	ACTGG	3 cut(s)	547, 717, 889
BseRI	GAGGAG	1 cut(s)	488
BseXI	GCAGC	5 cut(s)	441, 722, 1004, 1301, 1304
BsgI	GTGCAG	2 cut(s)	320, 1590
Bsh1236I	CGCG	1 cut(s)	1311
Bsh1285I	CGRYCG	1 cut(s)	31
BshFI	GGCC	3 cut(s)	1449, 1581, 1646
BshTI	ACCGGT	1 cut(s)	26
BsiEI	CGRYCG	1 cut(s)	31
BsiHKAI	GWGCWC	1 cut(s)	83
BsiHKCI	CYCGRG	1 cut(s)	439
BsiSI	CCGG	3 cut(s)	27, 90, 463
BslFI	GGGAC	2 cut(s)	877, 1304
BslI	CCNNNNNNNGG	2 cut(s)	533, 1096
BsmAI	GTCTC	1 cut(s)	1169
BsmFI	GGGAC	2 cut(s)	877, 1304
BsmI	GAATGC	2 cut(s)	313, 1295
BsnI	GGCC	3 cut(s)	1449, 1581, 1646
BsoBI	CYCGRG	1 cut(s)	439
Bsp1286I	GDGCHC	1 cut(s)	83
Bsp143I	GATC	4 cut(s)	67, 794, 923, 1390
Bsp19I	CCATGG	1 cut(s)	1236
BspACI	CCGC	5 cut(s)	111, 163, 555, 767, 1703
BspANI	GGCC	3 cut(s)	1449, 1581, 1646
BspCNI	CTCAG	3 cut(s)	9, 51, 463
BspFNI	CGCG	1 cut(s)	1311
BspLI	GGNNCC	2 cut(s)	808, 1668
BspPI	GGATC	2 cut(s)	802, 931
BsrFI	RCCGGY	1 cut(s)	26
BsrI	ACTGG	3 cut(s)	547, 717, 889
BssAI	RCCGGY	1 cut(s)	26
BssECI	CCNNGG	5 cut(s)	461, 694, 942, 1236, 1582
BssMI	GATC	4 cut(s)	67, 794, 923, 1390
BssNAI	GTATAC	1 cut(s)	638
BssT1I	CCWWGG	4 cut(s)	694, 942, 1236, 1582
Bst1107I	GTATAC	1 cut(s)	638
Bst4CI	ACNGT	3 cut(s)	500, 1494, 1633
BstC8I	GCNNGC	3 cut(s)	399, 1000, 1619
BstDEI	CTNAG	4 cut(s)	17, 38, 471, 718
BstDSI	CCRYGG	1 cut(s)	1236
BstF5I	GGATG	4 cut(s)	391, 426, 662, 1126
BstFNI	CGCG	1 cut(s)	1311
BstHHI	GCGC	3 cut(s)	780, 789, 1552
BstKTI	GATC	4 cut(s)	70, 797, 926, 1393
BstMAI	GTCTC	1 cut(s)	1169
BstMBI	GATC	4 cut(s)	67, 794, 923, 1390
BstMCI	CGRYCG	1 cut(s)	31
BstMWI	GCNNNNNNNGC	4 cut(s)	454, 784, 786, 995
BstNSI	RCATGY	3 cut(s)	186, 859, 1002
BstSCI	CCNGG	1 cut(s)	461
BstSFI	CTRYAG	2 cut(s)	1266, 1612
BstUI	CGCG	1 cut(s)	1311
BstV1I	GCAGC	5 cut(s)	441, 722, 1004, 1301, 1304
BstX2I	RGATCY	1 cut(s)	923
BstXI	CCANNNNNNTGG	2 cut(s)	514, 746
BstYI	RGATCY	1 cut(s)	923
BstZ17I	GTATAC	1 cut(s)	638
BsuI	GTATCC	1 cut(s)	244
BsuRI	GGCC	3 cut(s)	1449, 1581, 1646
BtgI	CCRYGG	1 cut(s)	1236
BtsCI	GGATG	4 cut(s)	391, 426, 662, 1126
BtsI	GCAGTG	1 cut(s)	1192
BtsIMutI	CAGTG	4 cut(s)	468, 710, 984, 1192
Cac8I	GCNNGC	3 cut(s)	399, 1000, 1619
CaiI	CAGNNNCTG	1 cut(s)	397
CfoI	GCGC	3 cut(s)	780, 789, 1552
Cfr10I	RCCGGY	1 cut(s)	26
Cfr13I	GGNCC	2 cut(s)	465, 1666
Csp6I	GTAC	2 cut(s)	430, 1387
CspAI	ACCGGT	1 cut(s)	26
CspCI	CAANNNNNGTGG	4 cut(s)	613, 648, 751, 786
CviAII	CATG	8 cut(s)	183, 803, 856, 999, 1091, 1232, 1237, 1577
CviQI	GTAC	2 cut(s)	430, 1387
DdeI	CTNAG	4 cut(s)	17, 38, 471, 718
DpnI	GATC	4 cut(s)	69, 796, 925, 1392
DpnII	GATC	4 cut(s)	67, 794, 923, 1390
EaeI	YGGCCR	1 cut(s)	1447
EciI	GGCGGA	1 cut(s)	570
Ecl136II	GAGCTC	1 cut(s)	81
Eco130I	CCWWGG	4 cut(s)	694, 942, 1236, 1582
Eco24I	GRGCYC	1 cut(s)	83
Eco32I	GATATC	1 cut(s)	203
Eco47I	GGWCC	2 cut(s)	465, 1666
Eco53kI	GAGCTC	1 cut(s)	81
Eco57I	CTGAAG	1 cut(s)	99
Eco88I	CYCGRG	1 cut(s)	439
EcoICRI	GAGCTC	1 cut(s)	81
EcoRI	GAATTC	2 cut(s)	328, 1076
EcoRV	GATATC	1 cut(s)	203
EcoT14I	CCWWGG	4 cut(s)	694, 942, 1236, 1582
EcoT22I	ATGCAT	1 cut(s)	662
EcoT38I	GRGCYC	1 cut(s)	83
ErhI	CCWWGG	4 cut(s)	694, 942, 1236, 1582
FaeI	CATG	8 cut(s)	186, 806, 859, 1002, 1094, 1235, 1240, 1580
FaqI	GGGAC	2 cut(s)	877, 1304
FatI	CATG	8 cut(s)	182, 802, 855, 998, 1090, 1231, 1236, 1576
FauI	CCCGC	1 cut(s)	760
FblI	GTMKAC	2 cut(s)	637, 1533
Fnu4HI	GCNGC	5 cut(s)	455, 736, 1018, 1290, 1293
FokI	GGATG	4 cut(s)	398, 433, 669, 1133
FriOI	GRGCYC	1 cut(s)	83
Fsp4HI	GCNGC	5 cut(s)	455, 736, 1018, 1290, 1293
FspBI	CTAG	2 cut(s)	1031, 1226
FspI	TGCGCA	1 cut(s)	1551
GlaI	GCGC	3 cut(s)	779, 788, 1551
GluI	GCNGC	5 cut(s)	455, 736, 1018, 1290, 1293
HaeIII	GGCC	3 cut(s)	1449, 1581, 1646
HapII	CCGG	3 cut(s)	27, 90, 463
HhaI	GCGC	3 cut(s)	780, 789, 1552
Hin1II	CATG	8 cut(s)	186, 806, 859, 1002, 1094, 1235, 1240, 1580
Hin6I	GCGC	3 cut(s)	778, 787, 1550
HinP1I	GCGC	3 cut(s)	778, 787, 1550
HindIII	AAGCTT	2 cut(s)	446, 1211
HinfI	GANTC	7 cut(s)	261, 487, 1060, 1111, 1317, 1424, 1715
HpaII	CCGG	3 cut(s)	27, 90, 463
HphI	GGTGA	5 cut(s)	39, 377, 640, 854, 1433
Hpy166II	GTNNAC	6 cut(s)	468, 638, 1351, 1534, 1542, 1636
Hpy188I	TCNGA	6 cut(s)	41, 334, 484, 675, 1110, 1258
Hpy188III	TCNNGA	6 cut(s)	245, 507, 821, 851, 1097, 1654
Hpy8I	GTNNAC	6 cut(s)	468, 638, 1351, 1534, 1542, 1636
Hpy99I	CGWCG	1 cut(s)	325
HpyAV	CCTTC	4 cut(s)	289, 914, 1231, 1235
HpyCH4III	ACNGT	3 cut(s)	500, 1494, 1633
HpyCH4IV	ACGT	1 cut(s)	323
HpyCH4V	TGCA	6 cut(s)	301, 424, 457, 660, 1471, 1571
HpyF10VI	GCNNNNNNNGC	4 cut(s)	454, 784, 786, 995
HpyF3I	CTNAG	4 cut(s)	17, 38, 471, 718
HpySE526I	ACGT	1 cut(s)	323
Hsp92II	CATG	8 cut(s)	186, 806, 859, 1002, 1094, 1235, 1240, 1580
HspAI	GCGC	3 cut(s)	778, 787, 1550
Kzo9I	GATC	4 cut(s)	67, 794, 923, 1390
LmnI	GCTCC	2 cut(s)	113, 1262
Lsp1109I	GCAGC	5 cut(s)	441, 722, 1004, 1301, 1304
LweI	GCATC	6 cut(s)	277, 411, 647, 1480, 1561, 1650
MaeI	CTAG	2 cut(s)	1031, 1226
MaeII	ACGT	1 cut(s)	323
MaeIII	GTNAC	1 cut(s)	937
MalI	GATC	4 cut(s)	69, 796, 925, 1392
MboI	GATC	4 cut(s)	67, 794, 923, 1390
MboII	GAAGA	3 cut(s)	382, 1669, 1691
MfeI	CAATTG	1 cut(s)	1710
MflI	RGATCY	1 cut(s)	923
MhlI	GDGCHC	1 cut(s)	83
MlsI	TGGCCA	1 cut(s)	1449
MluNI	TGGCCA	1 cut(s)	1449
MlyI	GAGTC	1 cut(s)	1326
MmeI	TCCRAC	1 cut(s)	1420
Mox20I	TGGCCA	1 cut(s)	1449
Mph1103I	ATGCAT	1 cut(s)	662
MroXI	GAANNNNTTC	2 cut(s)	215, 754
MscI	TGGCCA	1 cut(s)	1449
MseI	TTAA	2 cut(s)	492, 1476
MslI	CAYNNNNRTG	2 cut(s)	1119, 1476
Msp20I	TGGCCA	1 cut(s)	1449
MspA1I	CMGCKG	1 cut(s)	1725
MspI	CCGG	3 cut(s)	27, 90, 463
MspR9I	CCNGG	1 cut(s)	463
MunI	CAATTG	1 cut(s)	1710
Mva1269I	GAATGC	2 cut(s)	313, 1295
MvnI	CGCG	1 cut(s)	1311
MwoI	GCNNNNNNNGC	4 cut(s)	454, 784, 786, 995
NciI	CCSGG	1 cut(s)	463
NcoI	CCATGG	1 cut(s)	1236
NdeII	GATC	4 cut(s)	67, 794, 923, 1390
NlaIII	CATG	8 cut(s)	186, 806, 859, 1002, 1094, 1235, 1240, 1580
NlaIV	GGNNCC	2 cut(s)	808, 1668
NmuCI	GTSAC	1 cut(s)	937
NsbI	TGCGCA	1 cut(s)	1551
NsiI	ATGCAT	1 cut(s)	662
NspI	RCATGY	3 cut(s)	186, 859, 1002
PaeI	GCATGC	1 cut(s)	1002
PaeR7I	CTCGAG	1 cut(s)	439
PciI	ACATGT	1 cut(s)	182
PctI	GAATGC	2 cut(s)	313, 1295
PdmI	GAANNNNTTC	2 cut(s)	215, 754
PfeI	GAWTC	6 cut(s)	261, 487, 1060, 1111, 1424, 1715
PflMI	CCANNNNNTGG	1 cut(s)	1096
PinAI	ACCGGT	1 cut(s)	26
PkrI	GCNGC	5 cut(s)	456, 737, 1019, 1291, 1294
PleI	GAGTC	1 cut(s)	1325
PpsI	GAGTC	1 cut(s)	1325
PscI	ACATGT	1 cut(s)	182
Psp124BI	GAGCTC	1 cut(s)	83
PspN4I	GGNNCC	2 cut(s)	808, 1668
PspPI	GGNCC	2 cut(s)	465, 1666
PspXI	VCTCGAGB	1 cut(s)	439
PstNI	CAGNNNCTG	1 cut(s)	397
PsuI	RGATCY	1 cut(s)	923
PvuII	CAGCTG	1 cut(s)	1725
RsaI	GTAC	2 cut(s)	431, 1388
RsaNI	GTAC	2 cut(s)	430, 1387
RseI	CAYNNNNRTG	2 cut(s)	1119, 1476
SacI	GAGCTC	1 cut(s)	83
SaqAI	TTAA	2 cut(s)	492, 1476
SatI	GCNGC	5 cut(s)	455, 736, 1018, 1290, 1293
Sau3AI	GATC	4 cut(s)	67, 794, 923, 1390
Sau96I	GGNCC	2 cut(s)	465, 1666
SchI	GAGTC	1 cut(s)	1326
ScrFI	CCNGG	1 cut(s)	463
SduI	GDGCHC	1 cut(s)	83
SfaNI	GCATC	6 cut(s)	277, 411, 647, 1480, 1561, 1650
SfcI	CTRYAG	2 cut(s)	1266, 1612
Sfr274I	CTCGAG	1 cut(s)	439
SinI	GGWCC	2 cut(s)	465, 1666
SlaI	CTCGAG	1 cut(s)	439
SmiMI	CAYNNNNRTG	2 cut(s)	1119, 1476
SmlI	CTYRAG	3 cut(s)	245, 439, 528
SmoI	CTYRAG	3 cut(s)	245, 439, 528
SphI	GCATGC	1 cut(s)	1002
SsiI	CCGC	5 cut(s)	111, 163, 555, 767, 1703
SspMI	CTAG	2 cut(s)	1031, 1226
SstI	GAGCTC	1 cut(s)	83
StyD4I	CCNGG	1 cut(s)	461
StyI	CCWWGG	4 cut(s)	694, 942, 1236, 1582
TaaI	ACNGT	3 cut(s)	500, 1494, 1633
TaiI	ACGT	1 cut(s)	326
TaqI	TCGA	3 cut(s)	271, 320, 440
TaqII	GACCGA	1 cut(s)	305
TatI	WGTACW	1 cut(s)	429
TfiI	GAWTC	6 cut(s)	261, 487, 1060, 1111, 1424, 1715
Tru1I	TTAA	2 cut(s)	492, 1476
Tru9I	TTAA	2 cut(s)	492, 1476
TscAI	CASTG	4 cut(s)	475, 717, 984, 1192
TseFI	GTSAC	1 cut(s)	937
TseI	GCWGC	5 cut(s)	454, 735, 1017, 1289, 1292
Tsp45I	GTSAC	1 cut(s)	937
TspGWI	ACGGA	1 cut(s)	886
TspRI	CASTG	4 cut(s)	475, 717, 984, 1192
Van91I	CCANNNNNTGG	1 cut(s)	1096
VpaK11BI	GGWCC	2 cut(s)	465, 1666
XapI	RAATTY	3 cut(s)	214, 328, 1076
XceI	RCATGY	3 cut(s)	186, 859, 1002
XcmI	CCANNNNNNNNNTGG	2 cut(s)	817, 1457
XhoI	CTCGAG	1 cut(s)	439
XmiI	GTMKAC	2 cut(s)	637, 1533
XmnI	GAANNNNTTC	2 cut(s)	215, 754
XspI	CTAG	2 cut(s)	1031, 1226
Zsp2I	ATGCAT	1 cut(s)	662

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.