MD15G1271400.v1.1

BTB POZ domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0006702
Physical Location & Seq
Reverse (-)
23645716 .. 23649887
4172 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1271400.v1.1.491

Sequence Viewer

Length: 1611 bp
ATGGCTGAAATTAGGCTTACTAGGGTAGAACAAGGCAAAACAAAGATTAGAAATGTCCCAATTGCCGTGACGCCGGAAGGTTTCTGGTGTTGCCCTTCTCCTGTTGTGTTTCAGAAAACCCTTAAAGCTCAAAATTCCATGCACAAACCCAAGCCCTCATCACCACCACCACCCAAGGAAGCAGCTCAGAGAAAACAAACCCCACTGAGTGAAAAAAAGCTAGCCTTTACCCCTTCGAGGTCAGCAAGTATTTCTGATGGTCTACGGGGTGTTGGTCCTGAGGCACCTGATGCACCGGTGGTTAGTGCATCTATAGTTCCGGAGAGAATGCCTAAGCCCAAAGTTGAAAATTTGCCAAGGAAGGTAGCAATTGAGTTTGGTGAGCCTGGAACTAGTGATATGAAGGTGGTTTTACTTGGAAAGAAGGGATTTTGTGTAAAGTTGAGCGTTCACAAGAATGTTCTAGTGGAGCATAGCAACTTTTTTGCTGATAAACTTTCTGAACAACAAACTGGTTCAACTTGTCTTAACATCCAAGATTGCGATGATGTTGAAATATATGTTGAAACCGTTGGATTGATGTATTGCAAGGACATGAGGCAACGGCTGATGAAACAAAGTGTCTCGTGTGTACTCCGCATTCTAAAGATTGCAGAACTGCTTGGCTTCAACTCGTGCATGCAGTCGTGTTTAGAGTACTTGGAAGCAGTCCCGTGGGTTGGGGATGAAGAAGAAGAAAAAGTTGTCTCGTCAGTCTTACGACTCCAAAGTGAGGGTATTGGGGTCACTCCTGTACTGAAACGAGTCTCATCCGGCATTGCTAAACCCCAAAAGGACACACTTTCCCATATAATTGGACTTGTTCTCGCAAGCAATGAGGAGAGAGGCAGGCGTGAAATGAAATCCATAGTGCTGAAGCTTCTTAAGGAGAACAACGGTGCCTCAAGCTCTCGAGGTTCAGCTAACGTCTGCAATGAAACACTATATAACTCCTGCAGAAGCTGTTTGAACTTGCTGCTGTCCCTGTTCAAGCAGGCTGCAGAGCCCGAGTCTTCTGATAAAACTTTCGACAACAAAGAACCTGTGGTGAAGCAAATAGATTTGGCAGCTGATAACCTCTCATGGTTGCTTGAGATTTTGGCTGACAAGCAGGCAGCAGACGAATTTGCAGTAATGTGGGCTAGCCAGCAAGAATTGGCAGCCTTGCATACGAAGCTGCCTATTGTGTCTCGTTACCATGTTAGCCGTATCACGGCAAGGCTGTTTGTTGGCATTGGTAGAGGGGAGCTACTGCCATCTAAGGATACCCGTCAGCTGTTGCTACAAACCTGGCTACAGCCGTTGATCAACGACTACAACTGGCTGCAACATGGGTGCCGGTCGTTTGACCGTAAAGTTGTGGAAGAAGGAATTGGTAGGACAATCCTCACTCTGCCGCTAGAGGACCAGCAAAGTATTATGCTTTCGTGGTTGGGAAGTTTTCTCAAGGCTGGTGATAGCTGCCCGAATCTCCAGAGGGCGTTTGAAGTGTGGTGGCGGAGAACTTTTGTTAGACCATACGTGGAAGGACAAGGTAGCTCCGTCCAGTCAGATAGCTCATTGACCTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB

Protein Analysis

537

Amino Acids

59.59

Weight (kDa)

8.63

Isoelectric Point (pI)

56.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BTB-POZ_ANK-like PF25553 277 - 518 1.3e-64 BTB/POZ Ankyrin-like repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 283, 938, 1374
AccI GTMKAC 1 cut(s) 262
AccIII TCCGGA 1 cut(s) 319
AciI CCGC 3 cut(s) 637, 1436, 1537
AcsI RAATTY 3 cut(s) 133, 349, 1163
AcuI CTGAAG 1 cut(s) 935
AcyI GRCGYC 1 cut(s) 71
AdeI CACNNNGTG 1 cut(s) 209
AfaI GTAC 3 cut(s) 633, 698, 795
AfiI CCNNNNNNNGG 4 cut(s) 237, 719, 772, 1252
AflII CTTAAG 1 cut(s) 923
AgeI ACCGGT 1 cut(s) 295
AgsI TTSAA 8 cut(s) 347, 519, 554, 566, 670, 1009, 1030, 1526
AhlI ACTAGT 1 cut(s) 392
AjnI CCWGG 2 cut(s) 385, 1328
AjuI GAANNNNNNNTTGG 2 cut(s) 1395, 1427
Alw26I GTCTC 4 cut(s) 628, 751, 811, 1233
AlwNI CAGNNNCTG 1 cut(s) 1002
Ama87I CYCGRG 2 cut(s) 951, 1046
Aor13HI TCCGGA 1 cut(s) 319
ApeKI GCWGC 9 cut(s) 182, 1015, 1037, 1106, 1154, 1199, 1216, 1363, 1500
ApoI RAATTY 3 cut(s) 133, 349, 1163
AsiGI ACCGGT 1 cut(s) 295
AspS9I GGNCC 2 cut(s) 275, 1444
AsuHPI GGTGA 4 cut(s) 153, 392, 1099, 1505
AsuNHI GCTAGC 2 cut(s) 220, 1181
AvaI CYCGRG 2 cut(s) 951, 1046
AvaII GGWCC 2 cut(s) 275, 1444
AxyI CCTNAGG 1 cut(s) 279
BanI GGYRCC 3 cut(s) 283, 938, 1374
BanII GRGCYC 1 cut(s) 1047
BauI CACGAG 2 cut(s) 625, 673
BbsI GAAGAC 1 cut(s) 1044
BbvI GCAGC 9 cut(s) 194, 1002, 1024, 1118, 1166, 1203, 1211, 1350, 1487
BccI CCATC 2 cut(s) 251, 1303
BceAI ACGGC 5 cut(s) 50, 620, 1230, 1269, 1324
BciT130I CCWGG 2 cut(s) 387, 1330
BciVI GTATCC 1 cut(s) 1297
BclI TGATCA 1 cut(s) 1344
BcoDI GTCTC 4 cut(s) 628, 751, 811, 1233
BcuI ACTAGT 1 cut(s) 392
BfaI CTAG 6 cut(s) 21, 221, 393, 464, 1182, 1439
BfmI CTRYAG 4 cut(s) 312, 994, 1038, 1334
BfrI CTTAAG 1 cut(s) 923
BfuI GTATCC 1 cut(s) 1297
BmcAI AGTACT 1 cut(s) 698
Bme1390I CCNGG 2 cut(s) 387, 1330
Bme18I GGWCC 2 cut(s) 275, 1444
BmeT110I CYCGRG 2 cut(s) 951, 1046
BmgT120I GGNCC 2 cut(s) 275, 1444
BmiI GGNNCC 3 cut(s) 285, 940, 1376
BmrFI CCNGG 2 cut(s) 387, 1330
BmsI GCATC 2 cut(s) 280, 317
BmtI GCTAGC 2 cut(s) 224, 1185
BpiI GAAGAC 1 cut(s) 1044
BpmI CTGGAG 1 cut(s) 1496
Bpu10I CCTNAGC 1 cut(s) 333
BpuEI CTTGAG 3 cut(s) 928, 1151, 1469
BsaAI YACGTR 1 cut(s) 1561
BsaHI GRCGYC 1 cut(s) 71
BsaJI CCNNGG 3 cut(s) 174, 356, 713
BsaWI WCCGGW 2 cut(s) 295, 319
Bsc4I CCNNNNNNNGG 4 cut(s) 237, 719, 772, 1252
Bse118I RCCGGY 2 cut(s) 295, 1377
Bse1I ACTGG 3 cut(s) 517, 1364, 1585
Bse21I CCTNAGG 1 cut(s) 279
Bse3DI GCAATG 3 cut(s) 816, 880, 979
BseAI TCCGGA 1 cut(s) 319
BseBI CCWGG 2 cut(s) 387, 1330
BseDI CCNNGG 3 cut(s) 174, 356, 713
BseGI GGATG 3 cut(s) 531, 730, 809
BseLI CCNNNNNNNGG 4 cut(s) 237, 719, 772, 1252
BseMI GCAATG 3 cut(s) 816, 880, 979
BseMII CTCAG 3 cut(s) 197, 200, 270
BseNI ACTGG 3 cut(s) 517, 1364, 1585
BseRI GAGGAG 1 cut(s) 893
BseXI GCAGC 9 cut(s) 194, 1002, 1024, 1118, 1166, 1203, 1211, 1350, 1487
Bsh1285I CGRYCG 1 cut(s) 1382
BshNI GGYRCC 3 cut(s) 283, 938, 1374
BshTI ACCGGT 1 cut(s) 295
BsiEI CGRYCG 1 cut(s) 1382
BsiHKCI CYCGRG 2 cut(s) 951, 1046
BsiSI CCGG 5 cut(s) 74, 296, 320, 813, 1378
BslFI GGGAC 3 cut(s) 41, 695, 1006
BslI CCNNNNNNNGG 4 cut(s) 237, 719, 772, 1252
BsmAI GTCTC 4 cut(s) 628, 751, 811, 1233
BsmFI GGGAC 3 cut(s) 41, 695, 1006
BsmI GAATGC 2 cut(s) 333, 639
BsoBI CYCGRG 2 cut(s) 951, 1046
Bsp1286I GDGCHC 1 cut(s) 1047
Bsp13I TCCGGA 1 cut(s) 319
Bsp143I GATC 1 cut(s) 1344
BspACI CCGC 3 cut(s) 637, 1436, 1537
BspCNI CTCAG 3 cut(s) 198, 199, 271
BspEI TCCGGA 1 cut(s) 319
BspLI GGNNCC 3 cut(s) 285, 940, 1376
BspMAI CTGCAG 2 cut(s) 998, 1042
BspOI GCTAGC 2 cut(s) 224, 1185
BspT107I GGYRCC 3 cut(s) 283, 938, 1374
BspTI CTTAAG 1 cut(s) 923
BsrDI GCAATG 3 cut(s) 816, 880, 979
BsrFI RCCGGY 2 cut(s) 295, 1377
BsrI ACTGG 3 cut(s) 517, 1364, 1585
BssAI RCCGGY 2 cut(s) 295, 1377
BssECI CCNNGG 3 cut(s) 174, 356, 713
BssMI GATC 1 cut(s) 1344
BssNI GRCGYC 1 cut(s) 71
BssSI CACGAG 2 cut(s) 625, 673
BssT1I CCWWGG 2 cut(s) 174, 356
Bst2BI CACGAG 2 cut(s) 625, 673
Bst2UI CCWGG 2 cut(s) 387, 1330
Bst4CI ACNGT 3 cut(s) 571, 938, 1391
BstACI GRCGYC 1 cut(s) 71
BstAFI CTTAAG 1 cut(s) 923
BstAPI GCANNNNNTGC 1 cut(s) 290
BstBAI YACGTR 1 cut(s) 1561
BstC8I GCNNGC 8 cut(s) 222, 680, 871, 890, 1035, 1152, 1183, 1187
BstDEI CTNAG 5 cut(s) 186, 206, 279, 333, 1299
BstDSI CCRYGG 1 cut(s) 713
BstF5I GGATG 3 cut(s) 531, 730, 809
BstKTI GATC 1 cut(s) 1347
BstMAI GTCTC 4 cut(s) 628, 751, 811, 1233
BstMBI GATC 1 cut(s) 1344
BstMCI CGRYCG 1 cut(s) 1382
BstMWI GCNNNNNNNGC 2 cut(s) 290, 1213
BstNI CCWGG 2 cut(s) 387, 1330
BstNSI RCATGY 1 cut(s) 682
BstSCI CCNGG 2 cut(s) 385, 1328
BstSFI CTRYAG 4 cut(s) 312, 994, 1038, 1334
BstV1I GCAGC 9 cut(s) 194, 1002, 1024, 1118, 1166, 1203, 1211, 1350, 1487
BstV2I GAAGAC 1 cut(s) 1044
BstXI CCANNNNNNTGG 1 cut(s) 854
Bsu36I CCTNAGG 1 cut(s) 279
BsuI GTATCC 1 cut(s) 1297
BtgI CCRYGG 1 cut(s) 713
BtgZI GCGATG 1 cut(s) 558
BtsCI GGATG 3 cut(s) 531, 730, 809
BtsIMutI CAGTG 1 cut(s) 203
Cac8I GCNNGC 8 cut(s) 222, 680, 871, 890, 1035, 1152, 1183, 1187
CaiI CAGNNNCTG 1 cut(s) 1002
Cfr10I RCCGGY 2 cut(s) 295, 1377
Cfr13I GGNCC 2 cut(s) 275, 1444
CseI GACGC 1 cut(s) 79
Csp6I GTAC 3 cut(s) 632, 697, 794
CspAI ACCGGT 1 cut(s) 295
CviAII CATG 6 cut(s) 139, 595, 679, 1122, 1238, 1370
CviQI GTAC 3 cut(s) 632, 697, 794
DdeI CTNAG 5 cut(s) 186, 206, 279, 333, 1299
DpnI GATC 1 cut(s) 1346
DpnII GATC 1 cut(s) 1344
DraIII CACNNNGTG 1 cut(s) 209
EciI GGCGGA 1 cut(s) 1552
Eco130I CCWWGG 2 cut(s) 174, 356
Eco24I GRGCYC 1 cut(s) 1047
Eco47I GGWCC 2 cut(s) 275, 1444
Eco57I CTGAAG 1 cut(s) 935
Eco81I CCTNAGG 1 cut(s) 279
Eco88I CYCGRG 2 cut(s) 951, 1046
EcoRII CCWGG 2 cut(s) 385, 1328
EcoT14I CCWWGG 2 cut(s) 174, 356
EcoT38I GRGCYC 1 cut(s) 1047
ErhI CCWWGG 2 cut(s) 174, 356
FaeI CATG 6 cut(s) 142, 598, 682, 1125, 1241, 1373
FalI AAGNNNNNCTT 2 cut(s) 209, 241
FaqI GGGAC 3 cut(s) 41, 695, 1006
FatI CATG 6 cut(s) 138, 594, 678, 1121, 1237, 1369
FbaI TGATCA 1 cut(s) 1344
FblI GTMKAC 1 cut(s) 262
FokI GGATG 3 cut(s) 518, 737, 796
FriOI GRGCYC 1 cut(s) 1047
FspBI CTAG 6 cut(s) 21, 221, 393, 464, 1182, 1439
GsuI CTGGAG 1 cut(s) 1496
HapII CCGG 5 cut(s) 74, 296, 320, 813, 1378
HgaI GACGC 1 cut(s) 79
Hin1I GRCGYC 1 cut(s) 71
Hin1II CATG 6 cut(s) 142, 598, 682, 1125, 1241, 1373
HindIII AAGCTT 1 cut(s) 917
HinfI GANTC 4 cut(s) 762, 804, 1049, 1507
HpaII CCGG 5 cut(s) 74, 296, 320, 813, 1378
HphI GGTGA 4 cut(s) 153, 392, 1099, 1505
Hpy166II GTNNAC 3 cut(s) 263, 451, 632
Hpy188I TCNGA 6 cut(s) 114, 189, 256, 502, 1057, 1591
Hpy188III TCNNGA 4 cut(s) 278, 320, 951, 1513
Hpy8I GTNNAC 3 cut(s) 263, 451, 632
HpyAV CCTTC 8 cut(s) 71, 105, 243, 355, 397, 418, 1400, 1559
HpyCH4III ACNGT 3 cut(s) 571, 938, 1391
HpyCH4IV ACGT 2 cut(s) 966, 1560
HpyF10VI GCNNNNNNNGC 2 cut(s) 290, 1213
HpyF3I CTNAG 5 cut(s) 186, 206, 279, 333, 1299
HpySE526I ACGT 2 cut(s) 966, 1560
Hsp92I GRCGYC 1 cut(s) 71
Hsp92II CATG 6 cut(s) 142, 598, 682, 1125, 1241, 1373
Kpn2I TCCGGA 1 cut(s) 319
Ksp22I TGATCA 1 cut(s) 1344
Kzo9I GATC 1 cut(s) 1344
LmnI GCTCC 3 cut(s) 469, 1285, 1583
Lsp1109I GCAGC 9 cut(s) 194, 1002, 1024, 1118, 1166, 1203, 1211, 1350, 1487
LweI GCATC 2 cut(s) 280, 317
MaeI CTAG 6 cut(s) 21, 221, 393, 464, 1182, 1439
MaeII ACGT 2 cut(s) 966, 1560
MaeIII GTNAC 3 cut(s) 67, 784, 1232
MalI GATC 1 cut(s) 1346
MboI GATC 1 cut(s) 1344
MboII GAAGA 5 cut(s) 740, 743, 746, 1044, 1415
MfeI CAATTG 2 cut(s) 60, 369
MhlI GDGCHC 1 cut(s) 1047
MluCI AATT 9 cut(s) 9, 60, 133, 349, 369, 852, 1163, 1193, 1410
MlyI GAGTC 3 cut(s) 756, 813, 1058
MmeI TCCRAC 1 cut(s) 553
MroI TCCGGA 1 cut(s) 319
MseI TTAA 3 cut(s) 123, 528, 924
MslI CAYNNNNRTG 1 cut(s) 456
MspA1I CMGCKG 2 cut(s) 1109, 1315
MspCI CTTAAG 1 cut(s) 923
MspI CCGG 5 cut(s) 74, 296, 320, 813, 1378
MspR9I CCNGG 2 cut(s) 387, 1330
MunI CAATTG 2 cut(s) 60, 369
Mva1269I GAATGC 2 cut(s) 333, 639
MvaI CCWGG 2 cut(s) 387, 1330
MwoI GCNNNNNNNGC 2 cut(s) 290, 1213
NdeII GATC 1 cut(s) 1344
NheI GCTAGC 2 cut(s) 220, 1181
NlaIII CATG 6 cut(s) 142, 598, 682, 1125, 1241, 1373
NlaIV GGNNCC 3 cut(s) 285, 940, 1376
NmuCI GTSAC 2 cut(s) 67, 784
NspI RCATGY 1 cut(s) 682
PaeI GCATGC 1 cut(s) 682
PaeR7I CTCGAG 1 cut(s) 951
PctI GAATGC 2 cut(s) 333, 639
PfeI GAWTC 1 cut(s) 1507
PinAI ACCGGT 1 cut(s) 295
PleI GAGTC 3 cut(s) 756, 812, 1057
PpsI GAGTC 3 cut(s) 756, 812, 1057
Ppu21I YACGTR 1 cut(s) 1561
Psp6I CCWGG 2 cut(s) 385, 1328
PspGI CCWGG 2 cut(s) 385, 1328
PspN4I GGNNCC 3 cut(s) 285, 940, 1376
PspPI GGNCC 2 cut(s) 275, 1444
PstI CTGCAG 2 cut(s) 998, 1042
PstNI CAGNNNCTG 1 cut(s) 1002
PvuII CAGCTG 2 cut(s) 1109, 1315
RsaI GTAC 3 cut(s) 633, 698, 795
RsaNI GTAC 3 cut(s) 632, 697, 794
RseI CAYNNNNRTG 1 cut(s) 456
SaqAI TTAA 3 cut(s) 123, 528, 924
Sau3AI GATC 1 cut(s) 1344
Sau96I GGNCC 2 cut(s) 275, 1444
ScaI AGTACT 1 cut(s) 698
SchI GAGTC 3 cut(s) 756, 813, 1058
ScrFI CCNGG 2 cut(s) 387, 1330
SduI GDGCHC 1 cut(s) 1047
SfaNI GCATC 2 cut(s) 280, 317
SfcI CTRYAG 4 cut(s) 312, 994, 1038, 1334
Sfr274I CTCGAG 1 cut(s) 951
SgrAI CRCCGGYG 1 cut(s) 295
SinI GGWCC 2 cut(s) 275, 1444
SlaI CTCGAG 1 cut(s) 951
SmiMI CAYNNNNRTG 1 cut(s) 456
SmlI CTYRAG 5 cut(s) 923, 943, 951, 1130, 1484
SmoI CTYRAG 5 cut(s) 923, 943, 951, 1130, 1484
SpeI ACTAGT 1 cut(s) 392
SphI GCATGC 1 cut(s) 682
Sse9I AATT 9 cut(s) 9, 60, 133, 349, 369, 852, 1163, 1193, 1410
SsiI CCGC 3 cut(s) 637, 1436, 1537
SspMI CTAG 6 cut(s) 21, 221, 393, 464, 1182, 1439
StyD4I CCNGG 2 cut(s) 385, 1328
StyI CCWWGG 2 cut(s) 174, 356
TaaI ACNGT 3 cut(s) 571, 938, 1391
TaiI ACGT 2 cut(s) 969, 1563
TaqI TCGA 3 cut(s) 236, 952, 1068
TasI AATT 9 cut(s) 9, 60, 133, 349, 369, 852, 1163, 1193, 1410
TatI WGTACW 3 cut(s) 631, 696, 793
TauI GCSGC 1 cut(s) 1438
TfiI GAWTC 1 cut(s) 1507
Tru1I TTAA 3 cut(s) 123, 528, 924
Tru9I TTAA 3 cut(s) 123, 528, 924
TscAI CASTG 1 cut(s) 210
TseFI GTSAC 2 cut(s) 67, 784
TseI GCWGC 9 cut(s) 182, 1015, 1037, 1106, 1154, 1199, 1216, 1363, 1500
Tsp45I GTSAC 2 cut(s) 67, 784
TspDTI ATGAA 5 cut(s) 416, 626, 741, 914, 990
TspGWI ACGGA 1 cut(s) 1570
TspRI CASTG 1 cut(s) 210
Vha464I CTTAAG 1 cut(s) 923
VpaK11BI GGWCC 2 cut(s) 275, 1444
XapI RAATTY 3 cut(s) 133, 349, 1163
XceI RCATGY 1 cut(s) 682
XhoI CTCGAG 1 cut(s) 951
XmiI GTMKAC 1 cut(s) 262
XspI CTAG 6 cut(s) 21, 221, 393, 464, 1182, 1439
ZrmI AGTACT 1 cut(s) 698
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.